Rmu_sc0018644.1_g000003

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0018644.1
OG0000633
Physical Location & Seq
Forward (+)
10461 .. 11972
1512 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0018644.1_g000003.1.cds

Sequence Viewer

Length: 981 bp
atggcggagccgaaagaaatggcaggcaagggcagaaatgttgcccgagcgttaatattcactcctggcacttcatcaggcactcctggcgttaatattcacccctggcacgccaagtgtcgagatagaaacacacgaggacccattggccccgatttcagagctggaagccatgagttggaggatgagaacactgcacttgcttcggcactggaccaacgacaccagcacaatgaagcgctcactcaagccttcgctagagcctcgcatgtaacgatgataaaattaaacgctgccccagttacaggtgagaccggtatcagaccaaccacatccgtcggtaccggccaacgggtcatccacatacaggtagatttgtaccctgagagcctaaggcatatgccgccactgcctttgcccagaccccctcaaattgagccactggtaactgacccatacacgacactgctcttacaactgagcaactctctgaatgcccttcaagcaagggtggaggcagcggagaaccgggacacctggcgtcccctgacaacattcgaggaccgtccgggtcctttcaccaaagaggttagaggtgtaatacgctcaaatgcatcaaagccgctgaagatccactatacgggaattggggacccatatcaacatctgcaagcttttcgctcacaaacgagcgccaagggatacatggatgagggggataccgtcagacgagaaagaaaggccgctaacccgacgtccgtggtcagggatgagccaagaaagtgggaatgggcccaggatggaggacgatcaccccacaattccattaaggggaatagggagtcctcgtccagatctggtgggtactttggcactcagtttcaccaggaagaccgaactcaacagaaccgtaaaacaccacctccctcagtacgaggtattcactctcctaaacgcttcgtacgaaactatttggaatga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

326

Amino Acids

36.39

Weight (kDa)

9.9

Isoelectric Point (pI)

44.5

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000633)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g22376 FvH4_4g15485
rosa_laevigata RLG00000001772
rosa_multiflora Rmu_co7980664.1_g000001 Rmu_co7991850.1_g000001 Rmu_co8069086.1_g000001 Rmu_co8203420.1_g000001 Rmu_co8213206.1_g000001 Rmu_co8240317.1_g000001 Rmu_co8382323.1_g000001 Rmu_co8439419.1_g000001 Rmu_co8463919.1_g000001 Rmu_co8496399.1_g000001 Rmu_co8505885.1_g000001 Rmu_sc0000194.1_g000007 Rmu_sc0000194.1_g000008 Rmu_sc0000279.1_g000005 Rmu_sc0000367.1_g000177 Rmu_sc0000385.1_g000009 Rmu_sc0000423.1_g000016 Rmu_sc0000539.1_g000053 Rmu_sc0000779.1_g000017 Rmu_sc0000940.1_g000038 Rmu_sc0000942.1_g000025 Rmu_sc0001436.1_g000019 Rmu_sc0001477.1_g000004 Rmu_sc0001483.1_g000028 Rmu_sc0001518.1_g000005 Rmu_sc0001565.1_g000021 Rmu_sc0001655.1_g000010 Rmu_sc0001773.1_g000009 Rmu_sc0001782.1_g000037 Rmu_sc0001866.1_g000009 Rmu_sc0002054.1_g000022 Rmu_sc0002075.1_g000001 Rmu_sc0002264.1_g000016 Rmu_sc0002868.1_g000020 Rmu_sc0003033.1_g000062 Rmu_sc0003276.1_g000007 Rmu_sc0003486.1_g000003 Rmu_sc0003760.1_g000026 Rmu_sc0004391.1_g000020 Rmu_sc0004549.1_g000021 Rmu_sc0004835.1_g000002 Rmu_sc0005137.1_g000028 Rmu_sc0005178.1_g000022 Rmu_sc0005406.1_g000004 Rmu_sc0006059.1_g000064 Rmu_sc0006822.1_g000011 Rmu_sc0006997.1_g000014 Rmu_sc0007144.1_g000004 Rmu_sc0007321.1_g000001 Rmu_sc0007677.1_g000001 Rmu_sc0009120.1_g000003 Rmu_sc0009255.1_g000002 Rmu_sc0009427.1_g000001 Rmu_sc0009806.1_g000012 Rmu_sc0010048.1_g000010 Rmu_sc0011416.1_g000001 Rmu_sc0014844.1_g000011 Rmu_sc0018644.1_g000003 Rmu_sc0019345.1_g000006 Rmu_sc0025211.1_g000005 Rmu_sc0029888.1_g000001 Rmu_sc0035796.1_g000001 Rmu_ssc0000381.1_g000017
rosa_roxburghii Rroxscaffold_1G00018250 Rroxscaffold_2G00093100 Rroxscaffold_2G00101670 Rroxscaffold_3G00249740 Rroxscaffold_4G00310550 Rroxscaffold_4G00331340 Rroxscaffold_5G00337050 Rroxscaffold_5G00378590 Rroxscaffold_5G00378600 Rroxscaffold_6G00389000 Rroxscaffold_6G00401050
rosa_rugosa Rorug02G0247700

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 758
Acc65I GGTACC 1 cut(s) 341
AccB1I GGYRCC 1 cut(s) 341
AccB7I CCANNNNNTGG 1 cut(s) 178
AciI CCGC 5 cut(s) 5, 404, 521, 623, 744
AclWI GGATC 1 cut(s) 625
AcoI YGGCCR 1 cut(s) 346
AcuI CTGAAG 1 cut(s) 647
AcyI GRCGYC 2 cut(s) 541, 755
AfaI GTAC 5 cut(s) 343, 380, 866, 933, 963
AfeI AGCGCT 1 cut(s) 240
AfiI CCNNNNNNNGG 7 cut(s) 178, 305, 351, 367, 640, 765, 831
AgeI ACCGGT 1 cut(s) 314
AgsI TTSAA 1 cut(s) 503
AjnI CCWGG 6 cut(s) 64, 85, 104, 536, 795, 885
AluBI AGCT 2 cut(s) 164, 674
AluI AGCT 2 cut(s) 164, 674
Alw26I GTCTC 1 cut(s) 305
AlwI GGATC 1 cut(s) 625
Ama87I CYCGRG 1 cut(s) 45
Aor51HI AGCGCT 1 cut(s) 240
AoxI GGCC 4 cut(s) 148, 346, 741, 792
ApaI GGGCCC 1 cut(s) 796
ApeKI GCWGC 2 cut(s) 293, 518
AsiGI ACCGGT 1 cut(s) 314
Asp718I GGTACC 1 cut(s) 341
AspLEI GCGC 2 cut(s) 241, 695
AspS9I GGNCC 8 cut(s) 140, 149, 214, 562, 572, 652, 792, 793
AsuC2I CCSGG 2 cut(s) 530, 570
AsuHPI GGTGA 5 cut(s) 92, 320, 571, 804, 875
AvaI CYCGRG 1 cut(s) 45
AvaII GGWCC 5 cut(s) 140, 214, 562, 572, 652
AxyI CCTNAGG 1 cut(s) 392
BaeGI GKGCMC 1 cut(s) 796
BanI GGYRCC 1 cut(s) 341
BanII GRGCYC 1 cut(s) 796
BauI CACGAG 1 cut(s) 135
BbsI GAAGAC 1 cut(s) 897
BbvI GCAGC 2 cut(s) 280, 530
BccI CCATC 1 cut(s) 794
BcgI CGANNNNNNTGC 4 cut(s) 186, 220, 659, 693
BciT130I CCWGG 6 cut(s) 66, 87, 106, 538, 797, 887
BciVI GTATCC 2 cut(s) 695, 712
BcnI CCSGG 2 cut(s) 530, 570
BcoDI GTCTC 1 cut(s) 305
BfaI CTAG 1 cut(s) 258
BfoI RGCGCY 2 cut(s) 242, 696
BfuI GTATCC 2 cut(s) 695, 712
BglII AGATCT 1 cut(s) 854
BisI GCNGC 5 cut(s) 294, 404, 519, 623, 744
BlsI GCNGC 5 cut(s) 295, 405, 520, 624, 745
Bme1390I CCNGG 8 cut(s) 66, 87, 106, 530, 538, 570, 797, 887
Bme18I GGWCC 5 cut(s) 140, 214, 562, 572, 652
BmeT110I CYCGRG 1 cut(s) 45
BmgT120I GGNCC 8 cut(s) 140, 149, 214, 562, 572, 652, 792, 793
BmiI GGNNCC 8 cut(s) 9, 142, 151, 343, 573, 653, 654, 794
BmrFI CCNGG 8 cut(s) 66, 87, 106, 530, 538, 570, 797, 887
BmrI ACTGGG 1 cut(s) 293
BmsI GCATC 1 cut(s) 623
BmuI ACTGGG 1 cut(s) 293
BpiI GAAGAC 1 cut(s) 897
BplI GAGNNNNNCTC 2 cut(s) 472, 504
BpuEI CTTGAG 1 cut(s) 231
BpuMI CCSGG 2 cut(s) 530, 570
BsaHI GRCGYC 2 cut(s) 541, 755
BsaI GGTCTC 1 cut(s) 305
BsaJI CCNNGG 4 cut(s) 104, 696, 759, 795
BsaWI WCCGGW 1 cut(s) 314
BsaXI ACNNNNNCTCC 4 cut(s) 833, 863, 907, 937
Bsc4I CCNNNNNNNGG 7 cut(s) 178, 305, 351, 367, 640, 765, 831
Bse118I RCCGGY 2 cut(s) 314, 344
Bse1I ACTGG 3 cut(s) 216, 299, 447
Bse21I CCTNAGG 1 cut(s) 392
BseBI CCWGG 6 cut(s) 66, 87, 106, 538, 797, 887
BseDI CCNNGG 4 cut(s) 104, 696, 759, 795
BseGI GGATG 6 cut(s) 190, 332, 357, 715, 775, 805
BseLI CCNNNNNNNGG 7 cut(s) 178, 305, 351, 367, 640, 765, 831
BseMII CTCAG 4 cut(s) 375, 470, 890, 942
BseNI ACTGG 3 cut(s) 216, 299, 447
BseSI GKGCMC 1 cut(s) 796
BseXI GCAGC 2 cut(s) 280, 530
BsgI GTGCAG 1 cut(s) 180
BshFI GGCC 4 cut(s) 150, 348, 743, 794
BshNI GGYRCC 1 cut(s) 341
BshTI ACCGGT 1 cut(s) 314
BsiHKCI CYCGRG 1 cut(s) 45
BsiSI CCGG 4 cut(s) 315, 345, 529, 569
BsiWI CGTACG 1 cut(s) 961
BslFI GGGAC 3 cut(s) 528, 545, 665
BslI CCNNNNNNNGG 7 cut(s) 178, 305, 351, 367, 640, 765, 831
BsmAI GTCTC 1 cut(s) 305
BsmFI GGGAC 3 cut(s) 528, 545, 665
BsmI GAATGC 1 cut(s) 499
BsnI GGCC 4 cut(s) 150, 348, 743, 794
Bso31I GGTCTC 1 cut(s) 305
BsoBI CYCGRG 1 cut(s) 45
Bsp120I GGGCCC 1 cut(s) 792
Bsp1286I GDGCHC 1 cut(s) 796
Bsp143I GATC 3 cut(s) 630, 809, 854
BspACI CCGC 5 cut(s) 5, 404, 521, 623, 744
BspANI GGCC 4 cut(s) 150, 348, 743, 794
BspCNI CTCAG 4 cut(s) 376, 471, 889, 941
BspLI GGNNCC 8 cut(s) 9, 142, 151, 343, 573, 653, 654, 794
BspPI GGATC 1 cut(s) 625
BspT107I GGYRCC 1 cut(s) 341
BspTNI GGTCTC 1 cut(s) 305
BsrFI RCCGGY 2 cut(s) 314, 344
BsrI ACTGG 3 cut(s) 216, 299, 447
BssAI RCCGGY 2 cut(s) 314, 344
BssECI CCNNGG 4 cut(s) 104, 696, 759, 795
BssMI GATC 3 cut(s) 630, 809, 854
BssNI GRCGYC 2 cut(s) 541, 755
BssSI CACGAG 1 cut(s) 135
BssT1I CCWWGG 1 cut(s) 696
Bst2BI CACGAG 1 cut(s) 135
Bst2UI CCWGG 6 cut(s) 66, 87, 106, 538, 797, 887
Bst4CI ACNGT 3 cut(s) 566, 724, 911
BstACI GRCGYC 2 cut(s) 541, 755
BstC8I GCNNGC 3 cut(s) 25, 111, 672
BstDEI CTNAG 5 cut(s) 384, 392, 479, 876, 928
BstDSI CCRYGG 1 cut(s) 759
BstF5I GGATG 6 cut(s) 190, 332, 357, 715, 775, 805
BstH2I RGCGCY 2 cut(s) 242, 696
BstHHI GCGC 2 cut(s) 241, 695
BstKTI GATC 3 cut(s) 633, 812, 857
BstMAI GTCTC 1 cut(s) 305
BstMBI GATC 3 cut(s) 630, 809, 854
BstMWI GCNNNNNNNGC 4 cut(s) 87, 403, 409, 503
BstNI CCWGG 6 cut(s) 66, 87, 106, 538, 797, 887
BstNSI RCATGY 1 cut(s) 272
BstSCI CCNGG 8 cut(s) 64, 85, 104, 528, 536, 568, 795, 885
BstSLI GKGCMC 1 cut(s) 796
BstV1I GCAGC 2 cut(s) 280, 530
BstV2I GAAGAC 1 cut(s) 897
BstX2I RGATCY 2 cut(s) 630, 854
BstXI CCANNNNNNTGG 1 cut(s) 783
BstYI RGATCY 2 cut(s) 630, 854
Bsu36I CCTNAGG 1 cut(s) 392
BsuI GTATCC 2 cut(s) 695, 712
BsuRI GGCC 4 cut(s) 150, 348, 743, 794
BtgI CCRYGG 1 cut(s) 759
BtsCI GGATG 6 cut(s) 190, 332, 357, 715, 775, 805
BtsI GCAGTG 3 cut(s) 192, 407, 464
BtsIMutI CAGTG 5 cut(s) 192, 209, 407, 440, 464
Cac8I GCNNGC 3 cut(s) 25, 111, 672
CfoI GCGC 2 cut(s) 241, 695
Cfr10I RCCGGY 2 cut(s) 314, 344
Cfr13I GGNCC 8 cut(s) 140, 149, 214, 562, 572, 652, 792, 793
CseI GACGC 1 cut(s) 530
Csp6I GTAC 5 cut(s) 342, 379, 865, 932, 962
CspAI ACCGGT 1 cut(s) 314
CspCI CAANNNNNGTGG 2 cut(s) 396, 431
CviAII CATG 3 cut(s) 173, 269, 706
CviQI GTAC 5 cut(s) 342, 379, 865, 932, 962
DdeI CTNAG 5 cut(s) 384, 392, 479, 876, 928
DpnI GATC 3 cut(s) 632, 811, 856
DpnII GATC 3 cut(s) 630, 809, 854
EaeI YGGCCR 1 cut(s) 346
EciI GGCGGA 1 cut(s) 20
Eco130I CCWWGG 1 cut(s) 696
Eco24I GRGCYC 1 cut(s) 796
Eco31I GGTCTC 1 cut(s) 305
Eco47I GGWCC 5 cut(s) 140, 214, 562, 572, 652
Eco47III AGCGCT 1 cut(s) 240
Eco57I CTGAAG 1 cut(s) 647
Eco81I CCTNAGG 1 cut(s) 392
Eco88I CYCGRG 1 cut(s) 45
EcoO109I RGGNCCY 3 cut(s) 140, 572, 652
EcoRII CCWGG 6 cut(s) 64, 85, 104, 536, 795, 885
EcoT14I CCWWGG 1 cut(s) 696
EcoT22I ATGCAT 1 cut(s) 616
EcoT38I GRGCYC 1 cut(s) 796
ErhI CCWWGG 1 cut(s) 696
FaeI CATG 3 cut(s) 176, 272, 709
FaiI YATR 9 cut(s) 174, 270, 365, 399, 401, 457, 639, 658, 707
FaqI GGGAC 3 cut(s) 528, 545, 665
FatI CATG 3 cut(s) 172, 268, 705
FauNDI CATATG 1 cut(s) 399
Fnu4HI GCNGC 5 cut(s) 294, 404, 519, 623, 744
FokI GGATG 6 cut(s) 197, 319, 344, 722, 782, 812
FriOI GRGCYC 1 cut(s) 796
Fsp4HI GCNGC 5 cut(s) 294, 404, 519, 623, 744
FspBI CTAG 1 cut(s) 258
GlaI GCGC 2 cut(s) 240, 694
GluI GCNGC 5 cut(s) 294, 404, 519, 623, 744
HaeII RGCGCY 2 cut(s) 242, 696
HaeIII GGCC 4 cut(s) 150, 348, 743, 794
HapII CCGG 4 cut(s) 315, 345, 529, 569
HgaI GACGC 1 cut(s) 530
HhaI GCGC 2 cut(s) 241, 695
Hin1I GRCGYC 2 cut(s) 541, 755
Hin1II CATG 3 cut(s) 176, 272, 709
Hin6I GCGC 2 cut(s) 239, 693
HinP1I GCGC 2 cut(s) 239, 693
HindIII AAGCTT 1 cut(s) 672
HinfI GANTC 1 cut(s) 842
HpaII CCGG 4 cut(s) 315, 345, 529, 569
HphI GGTGA 5 cut(s) 92, 320, 571, 804, 875
Hpy188I TCNGA 4 cut(s) 161, 323, 492, 728
Hpy188III TCNNGA 2 cut(s) 122, 852
Hpy99I CGWCG 2 cut(s) 341, 757
HpyAV CCTTC 2 cut(s) 262, 509
HpyCH4III ACNGT 3 cut(s) 566, 724, 911
HpyCH4IV ACGT 1 cut(s) 755
HpyCH4V TGCA 3 cut(s) 197, 614, 670
HpyF10VI GCNNNNNNNGC 4 cut(s) 87, 403, 409, 503
HpyF3I CTNAG 5 cut(s) 384, 392, 479, 876, 928
HpySE526I ACGT 1 cut(s) 755
Hsp92I GRCGYC 2 cut(s) 541, 755
Hsp92II CATG 3 cut(s) 176, 272, 709
HspAI GCGC 2 cut(s) 239, 693
KflI GGGWCCC 1 cut(s) 652
KpnI GGTACC 1 cut(s) 345
Kzo9I GATC 3 cut(s) 630, 809, 854
LmnI GCTCC 1 cut(s) 7
Lsp1109I GCAGC 2 cut(s) 280, 530
LweI GCATC 1 cut(s) 623
MaeI CTAG 1 cut(s) 258
MaeII ACGT 1 cut(s) 755
MaeIII GTNAC 3 cut(s) 271, 301, 445
MalI GATC 3 cut(s) 632, 811, 856
MboI GATC 3 cut(s) 630, 809, 854
MboII GAAGA 2 cut(s) 640, 902
MflI RGATCY 2 cut(s) 630, 854
MhlI GDGCHC 1 cut(s) 796
MluCI AATT 4 cut(s) 284, 432, 645, 820
MlyI GAGTC 1 cut(s) 851
MmeI TCCRAC 1 cut(s) 159
Mph1103I ATGCAT 1 cut(s) 616
MseI TTAA 4 cut(s) 53, 93, 287, 828
MspA1I CMGCKG 2 cut(s) 521, 625
MspI CCGG 4 cut(s) 315, 345, 529, 569
MspR9I CCNGG 8 cut(s) 66, 87, 106, 530, 538, 570, 797, 887
Mva1269I GAATGC 1 cut(s) 499
MvaI CCWGG 6 cut(s) 66, 87, 106, 538, 797, 887
MwoI GCNNNNNNNGC 4 cut(s) 87, 403, 409, 503
NciI CCSGG 2 cut(s) 530, 570
NdeI CATATG 1 cut(s) 399
NdeII GATC 3 cut(s) 630, 809, 854
NlaIII CATG 3 cut(s) 176, 272, 709
NlaIV GGNNCC 8 cut(s) 9, 142, 151, 343, 573, 653, 654, 794
NsiI ATGCAT 1 cut(s) 616
NspI RCATGY 1 cut(s) 272
PcsI WCGNNNNNNNCGW 2 cut(s) 272, 961
PctI GAATGC 1 cut(s) 499
Pfl23II CGTACG 1 cut(s) 961
PflMI CCANNNNNTGG 1 cut(s) 178
PinAI ACCGGT 1 cut(s) 314
PkrI GCNGC 5 cut(s) 295, 405, 520, 624, 745
PleI GAGTC 1 cut(s) 850
PpsI GAGTC 1 cut(s) 850
PpuMI RGGWCCY 3 cut(s) 140, 572, 652
Psp5II RGGWCCY 3 cut(s) 140, 572, 652
Psp6I CCWGG 6 cut(s) 64, 85, 104, 536, 795, 885
PspGI CCWGG 6 cut(s) 64, 85, 104, 536, 795, 885
PspLI CGTACG 1 cut(s) 961
PspN4I GGNNCC 8 cut(s) 9, 142, 151, 343, 573, 653, 654, 794
PspOMI GGGCCC 1 cut(s) 792
PspPI GGNCC 8 cut(s) 140, 149, 214, 562, 572, 652, 792, 793
PspPPI RGGWCCY 3 cut(s) 140, 572, 652
PsuI RGATCY 2 cut(s) 630, 854
RsaI GTAC 5 cut(s) 343, 380, 866, 933, 963
RsaNI GTAC 5 cut(s) 342, 379, 865, 932, 962
SaqAI TTAA 4 cut(s) 53, 93, 287, 828
SatI GCNGC 5 cut(s) 294, 404, 519, 623, 744
Sau3AI GATC 3 cut(s) 630, 809, 854
Sau96I GGNCC 8 cut(s) 140, 149, 214, 562, 572, 652, 792, 793
SchI GAGTC 1 cut(s) 851
ScrFI CCNGG 8 cut(s) 66, 87, 106, 530, 538, 570, 797, 887
SduI GDGCHC 1 cut(s) 796
SfaNI GCATC 1 cut(s) 623
SinI GGWCC 5 cut(s) 140, 214, 562, 572, 652
SmlI CTYRAG 1 cut(s) 246
SmoI CTYRAG 1 cut(s) 246
Sse9I AATT 4 cut(s) 284, 432, 645, 820
SsiI CCGC 5 cut(s) 5, 404, 521, 623, 744
SspI AATATT 2 cut(s) 57, 97
SspMI CTAG 1 cut(s) 258
StyD4I CCNGG 8 cut(s) 64, 85, 104, 528, 536, 568, 795, 885
StyI CCWWGG 1 cut(s) 696
TaaI ACNGT 3 cut(s) 566, 724, 911
TaiI ACGT 1 cut(s) 758
TaqI TCGA 2 cut(s) 121, 558
TaqII GACCGA 1 cut(s) 909
TasI AATT 4 cut(s) 284, 432, 645, 820
TauI GCSGC 3 cut(s) 406, 625, 746
Tru1I TTAA 4 cut(s) 53, 93, 287, 828
Tru9I TTAA 4 cut(s) 53, 93, 287, 828
TscAI CASTG 5 cut(s) 199, 216, 414, 447, 471
TseI GCWGC 2 cut(s) 293, 518
TspDTI ATGAA 2 cut(s) 63, 249
TspGWI ACGGA 2 cut(s) 325, 748
TspRI CASTG 5 cut(s) 199, 216, 414, 447, 471
Van91I CCANNNNNTGG 1 cut(s) 178
VpaK11BI GGWCC 5 cut(s) 140, 214, 562, 572, 652
XceI RCATGY 1 cut(s) 272
XcmI CCANNNNNNNNNTGG 1 cut(s) 703
XspI CTAG 1 cut(s) 258
ZraI GACGTC 1 cut(s) 756
Zsp2I ATGCAT 1 cut(s) 616
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.