heat shock
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc36I | ACCTGC | 1 cut(s) | 310 |
| Acc65I | GGTACC | 1 cut(s) | 44 |
| AccB1I | GGYRCC | 2 cut(s) | 44, 1883 |
| AccI | GTMKAC | 1 cut(s) | 1950 |
| AccIII | TCCGGA | 1 cut(s) | 1403 |
| AciI | CCGC | 4 cut(s) | 546, 549, 1161, 1923 |
| AclI | AACGTT | 1 cut(s) | 516 |
| AclWI | GGATC | 3 cut(s) | 948, 1252, 1330 |
| AcoI | YGGCCR | 1 cut(s) | 278 |
| AcsI | RAATTY | 2 cut(s) | 1391, 1407 |
| AcuI | CTGAAG | 2 cut(s) | 931, 1902 |
| AfaI | GTAC | 4 cut(s) | 46, 1346, 1592, 1850 |
| AfiI | CCNNNNNNNGG | 4 cut(s) | 29, 1117, 1225, 1919 |
| AgeI | ACCGGT | 1 cut(s) | 779 |
| AjnI | CCWGG | 4 cut(s) | 1068, 1143, 1338, 1879 |
| AleI | CACNNNNGTG | 1 cut(s) | 81 |
| Alw21I | GWGCWC | 5 cut(s) | 796, 933, 1007, 1401, 1614 |
| Alw26I | GTCTC | 3 cut(s) | 643, 1486, 1550 |
| AlwI | GGATC | 3 cut(s) | 948, 1252, 1330 |
| Ama87I | CYCGRG | 2 cut(s) | 1643, 1760 |
| Aor13HI | TCCGGA | 1 cut(s) | 1403 |
| AoxI | GGCC | 1 cut(s) | 278 |
| ApeKI | GCWGC | 4 cut(s) | 170, 356, 1136, 1709 |
| ApoI | RAATTY | 2 cut(s) | 1391, 1407 |
| AsiGI | ACCGGT | 1 cut(s) | 779 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 350 |
| Asp718I | GGTACC | 1 cut(s) | 44 |
| AspS9I | GGNCC | 4 cut(s) | 20, 299, 1877, 1931 |
| AsuC2I | CCSGG | 2 cut(s) | 1326, 1706 |
| AsuII | TTCGAA | 1 cut(s) | 1794 |
| AvaI | CYCGRG | 2 cut(s) | 1643, 1760 |
| AvaII | GGWCC | 4 cut(s) | 20, 299, 1877, 1931 |
| AxyI | CCTNAGG | 1 cut(s) | 1050 |
| BaeI | ACNNNNGTAYC | 2 cut(s) | 871, 904 |
| BalI | TGGCCA | 1 cut(s) | 280 |
| BanI | GGYRCC | 2 cut(s) | 44, 1883 |
| BanII | GRGCYC | 3 cut(s) | 796, 933, 1401 |
| BauI | CACGAG | 1 cut(s) | 1422 |
| BbsI | GAAGAC | 4 cut(s) | 971, 1291, 1608, 1803 |
| Bbv12I | GWGCWC | 5 cut(s) | 796, 933, 1007, 1401, 1614 |
| BbvCI | CCTCAGC | 4 cut(s) | 360, 405, 1488, 1779 |
| BbvI | GCAGC | 4 cut(s) | 182, 368, 1123, 1696 |
| BccI | CCATC | 9 cut(s) | 133, 705, 869, 914, 988, 1283, 1532, 1564, 1682 |
| BceAI | ACGGC | 2 cut(s) | 422, 1099 |
| BciT130I | CCWGG | 4 cut(s) | 1070, 1145, 1340, 1881 |
| BciVI | GTATCC | 2 cut(s) | 1055, 1481 |
| BclI | TGATCA | 1 cut(s) | 103 |
| BcnI | CCSGG | 2 cut(s) | 1326, 1706 |
| BcoDI | GTCTC | 3 cut(s) | 643, 1486, 1550 |
| BfaI | CTAG | 1 cut(s) | 918 |
| BfmI | CTRYAG | 2 cut(s) | 540, 1710 |
| BfuAI | ACCTGC | 1 cut(s) | 310 |
| BfuI | GTATCC | 2 cut(s) | 1055, 1481 |
| BglII | AGATCT | 1 cut(s) | 1186 |
| BisI | GCNGC | 6 cut(s) | 171, 357, 546, 549, 1137, 1710 |
| BlsI | GCNGC | 6 cut(s) | 172, 358, 547, 550, 1138, 1711 |
| Bme1390I | CCNGG | 6 cut(s) | 1070, 1145, 1326, 1340, 1706, 1881 |
| Bme18I | GGWCC | 4 cut(s) | 20, 299, 1877, 1931 |
| BmeT110I | CYCGRG | 2 cut(s) | 1643, 1760 |
| BmgT120I | GGNCC | 4 cut(s) | 20, 299, 1877, 1931 |
| BmiI | GGNNCC | 3 cut(s) | 46, 963, 1885 |
| BmrFI | CCNGG | 6 cut(s) | 1070, 1145, 1326, 1340, 1706, 1881 |
| BmsI | GCATC | 6 cut(s) | 381, 481, 976, 1450, 1830, 1894 |
| BoxI | GACNNNNGTC | 1 cut(s) | 1539 |
| BpiI | GAAGAC | 4 cut(s) | 971, 1291, 1608, 1803 |
| BplI | GAGNNNNNCTC | 4 cut(s) | 857, 889, 1140, 1172 |
| BpmI | CTGGAG | 2 cut(s) | 1052, 1398 |
| Bpu10I | CCTNAGC | 4 cut(s) | 360, 405, 1488, 1779 |
| Bpu14I | TTCGAA | 1 cut(s) | 1794 |
| BpuMI | CCSGG | 2 cut(s) | 1326, 1706 |
| Bsa29I | ATCGAT | 1 cut(s) | 36 |
| BsaBI | GATNNNNATC | 1 cut(s) | 1523 |
| BsaJI | CCNNGG | 8 cut(s) | 375, 415, 1144, 1219, 1262, 1368, 1551, 1852 |
| BsaWI | WCCGGW | 3 cut(s) | 779, 1403, 1502 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 884, 914 |
| Bsc4I | CCNNNNNNNGG | 4 cut(s) | 29, 1117, 1225, 1919 |
| Bse118I | RCCGGY | 3 cut(s) | 779, 1235, 1871 |
| Bse1I | ACTGG | 2 cut(s) | 965, 1771 |
| Bse21I | CCTNAGG | 1 cut(s) | 1050 |
| Bse3DI | GCAATG | 2 cut(s) | 93, 1836 |
| Bse8I | GATNNNNATC | 1 cut(s) | 1523 |
| BseAI | TCCGGA | 1 cut(s) | 1403 |
| BseBI | CCWGG | 4 cut(s) | 1070, 1145, 1340, 1881 |
| BseCI | ATCGAT | 1 cut(s) | 36 |
| BseDI | CCNNGG | 8 cut(s) | 375, 415, 1144, 1219, 1262, 1368, 1551, 1852 |
| BseGI | GGATG | 4 cut(s) | 496, 1275, 1561, 1674 |
| BseJI | GATNNNNATC | 1 cut(s) | 1523 |
| BseLI | CCNNNNNNNGG | 4 cut(s) | 29, 1117, 1225, 1919 |
| BseMI | GCAATG | 2 cut(s) | 93, 1836 |
| BseMII | CTCAG | 9 cut(s) | 351, 396, 485, 866, 1171, 1326, 1479, 1575, 1770 |
| BseNI | ACTGG | 2 cut(s) | 965, 1771 |
| BseRI | GAGGAG | 5 cut(s) | 377, 941, 1200, 1410, 1622 |
| BseXI | GCAGC | 4 cut(s) | 182, 368, 1123, 1696 |
| Bsh1285I | CGRYCG | 2 cut(s) | 82, 235 |
| BshFI | GGCC | 1 cut(s) | 280 |
| BshNI | GGYRCC | 2 cut(s) | 44, 1883 |
| BshTI | ACCGGT | 1 cut(s) | 779 |
| BshVI | ATCGAT | 1 cut(s) | 36 |
| BsiEI | CGRYCG | 2 cut(s) | 82, 235 |
| BsiHKAI | GWGCWC | 5 cut(s) | 796, 933, 1007, 1401, 1614 |
| BsiHKCI | CYCGRG | 2 cut(s) | 1643, 1760 |
| BsiSI | CCGG | 8 cut(s) | 297, 780, 1236, 1326, 1404, 1503, 1706, 1872 |
| BslFI | GGGAC | 1 cut(s) | 1415 |
| BslI | CCNNNNNNNGG | 4 cut(s) | 29, 1117, 1225, 1919 |
| BsmAI | GTCTC | 3 cut(s) | 643, 1486, 1550 |
| BsmBI | CGTCTC | 1 cut(s) | 1550 |
| BsmFI | GGGAC | 1 cut(s) | 1415 |
| BsnI | GGCC | 1 cut(s) | 280 |
| BsoBI | CYCGRG | 2 cut(s) | 1643, 1760 |
| Bsp119I | TTCGAA | 1 cut(s) | 1794 |
| Bsp1286I | GDGCHC | 5 cut(s) | 796, 933, 1007, 1401, 1614 |
| Bsp13I | TCCGGA | 1 cut(s) | 1403 |
| Bsp19I | CCATGG | 1 cut(s) | 375 |
| BspACI | CCGC | 4 cut(s) | 546, 549, 1161, 1923 |
| BspANI | GGCC | 1 cut(s) | 280 |
| BspCNI | CTCAG | 9 cut(s) | 352, 397, 484, 865, 1170, 1325, 1480, 1576, 1771 |
| BspDI | ATCGAT | 1 cut(s) | 36 |
| BspEI | TCCGGA | 1 cut(s) | 1403 |
| BspHI | TCATGA | 2 cut(s) | 1012, 1245 |
| BspLI | GGNNCC | 3 cut(s) | 46, 963, 1885 |
| BspMAI | CTGCAG | 1 cut(s) | 1714 |
| BspMI | ACCTGC | 1 cut(s) | 310 |
| BspPI | GGATC | 3 cut(s) | 948, 1252, 1330 |
| BspT104I | TTCGAA | 1 cut(s) | 1794 |
| BspT107I | GGYRCC | 2 cut(s) | 44, 1883 |
| BsrDI | GCAATG | 2 cut(s) | 93, 1836 |
| BsrFI | RCCGGY | 3 cut(s) | 779, 1235, 1871 |
| BsrI | ACTGG | 2 cut(s) | 965, 1771 |
| BssAI | RCCGGY | 3 cut(s) | 779, 1235, 1871 |
| BssECI | CCNNGG | 8 cut(s) | 375, 415, 1144, 1219, 1262, 1368, 1551, 1852 |
| BssSI | CACGAG | 1 cut(s) | 1422 |
| BssT1I | CCWWGG | 7 cut(s) | 375, 415, 1219, 1262, 1368, 1551, 1852 |
| Bst2BI | CACGAG | 1 cut(s) | 1422 |
| Bst2UI | CCWGG | 4 cut(s) | 1070, 1145, 1340, 1881 |
| Bst4CI | ACNGT | 7 cut(s) | 83, 208, 448, 563, 1009, 1133, 1444 |
| Bst6I | CTCTTC | 6 cut(s) | 336, 648, 651, 755, 824, 950 |
| BstBI | TTCGAA | 1 cut(s) | 1794 |
| BstC8I | GCNNGC | 1 cut(s) | 1746 |
| BstDSI | CCRYGG | 1 cut(s) | 375 |
| BstEII | GGTNACC | 2 cut(s) | 782, 1766 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 1917 |
| BstF5I | GGATG | 4 cut(s) | 496, 1275, 1561, 1674 |
| BstMAI | GTCTC | 3 cut(s) | 643, 1486, 1550 |
| BstMCI | CGRYCG | 2 cut(s) | 82, 235 |
| BstMWI | GCNNNNNNNGC | 5 cut(s) | 302, 554, 1133, 1637, 1913 |
| BstNI | CCWGG | 4 cut(s) | 1070, 1145, 1340, 1881 |
| BstPAI | GACNNNNGTC | 1 cut(s) | 1539 |
| BstPI | GGTNACC | 2 cut(s) | 782, 1766 |
| BstSCI | CCNGG | 6 cut(s) | 1068, 1143, 1324, 1338, 1704, 1879 |
| BstSFI | CTRYAG | 2 cut(s) | 540, 1710 |
| BstV1I | GCAGC | 4 cut(s) | 182, 368, 1123, 1696 |
| BstV2I | GAAGAC | 4 cut(s) | 971, 1291, 1608, 1803 |
| BstX2I | RGATCY | 2 cut(s) | 940, 1186 |
| BstXI | CCANNNNNNTGG | 2 cut(s) | 704, 1859 |
| BstYI | RGATCY | 2 cut(s) | 940, 1186 |
| Bsu15I | ATCGAT | 1 cut(s) | 36 |
| Bsu36I | CCTNAGG | 1 cut(s) | 1050 |
| BsuI | GTATCC | 2 cut(s) | 1055, 1481 |
| BsuRI | GGCC | 1 cut(s) | 280 |
| BsuTUI | ATCGAT | 1 cut(s) | 36 |
| BtgI | CCRYGG | 1 cut(s) | 375 |
| BtgZI | GCGATG | 1 cut(s) | 487 |
| BtsCI | GGATG | 4 cut(s) | 496, 1275, 1561, 1674 |
| BtsI | GCAGTG | 1 cut(s) | 846 |
| BtsIMutI | CAGTG | 2 cut(s) | 846, 1005 |
| BveI | ACCTGC | 1 cut(s) | 310 |
| Cac8I | GCNNGC | 1 cut(s) | 1746 |
| CciI | TCATGA | 2 cut(s) | 1012, 1245 |
| Cfr10I | RCCGGY | 3 cut(s) | 779, 1235, 1871 |
| Cfr13I | GGNCC | 4 cut(s) | 20, 299, 1877, 1931 |
| ClaI | ATCGAT | 1 cut(s) | 36 |
| CseI | GACGC | 4 cut(s) | 49, 176, 224, 464 |
| Csp6I | GTAC | 4 cut(s) | 45, 1345, 1591, 1849 |
| CspAI | ACCGGT | 1 cut(s) | 779 |
| CviAII | CATG | 7 cut(s) | 190, 268, 376, 937, 1013, 1246, 1663 |
| CviQI | GTAC | 4 cut(s) | 45, 1345, 1591, 1849 |
| EaeI | YGGCCR | 1 cut(s) | 278 |
| Eam1104I | CTCTTC | 6 cut(s) | 336, 648, 651, 755, 824, 950 |
| EarI | CTCTTC | 6 cut(s) | 336, 648, 651, 755, 824, 950 |
| Ecl136II | GAGCTC | 3 cut(s) | 794, 931, 1399 |
| Eco130I | CCWWGG | 7 cut(s) | 375, 415, 1219, 1262, 1368, 1551, 1852 |
| Eco24I | GRGCYC | 3 cut(s) | 796, 933, 1401 |
| Eco32I | GATATC | 1 cut(s) | 775 |
| Eco47I | GGWCC | 4 cut(s) | 20, 299, 1877, 1931 |
| Eco53kI | GAGCTC | 3 cut(s) | 794, 931, 1399 |
| Eco57I | CTGAAG | 2 cut(s) | 931, 1902 |
| Eco81I | CCTNAGG | 1 cut(s) | 1050 |
| Eco88I | CYCGRG | 2 cut(s) | 1643, 1760 |
| Eco91I | GGTNACC | 2 cut(s) | 782, 1766 |
| EcoICRI | GAGCTC | 3 cut(s) | 794, 931, 1399 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 1917 |
| EcoO65I | GGTNACC | 2 cut(s) | 782, 1766 |
| EcoRI | GAATTC | 1 cut(s) | 1407 |
| EcoRII | CCWGG | 4 cut(s) | 1068, 1143, 1338, 1879 |
| EcoRV | GATATC | 1 cut(s) | 775 |
| EcoT14I | CCWWGG | 7 cut(s) | 375, 415, 1219, 1262, 1368, 1551, 1852 |
| EcoT38I | GRGCYC | 3 cut(s) | 796, 933, 1401 |
| ErhI | CCWWGG | 7 cut(s) | 375, 415, 1219, 1262, 1368, 1551, 1852 |
| Esp3I | CGTCTC | 1 cut(s) | 1550 |
| FaeI | CATG | 7 cut(s) | 193, 271, 379, 940, 1016, 1249, 1666 |
| FaqI | GGGAC | 1 cut(s) | 1415 |
| FatI | CATG | 7 cut(s) | 189, 267, 375, 936, 1012, 1245, 1662 |
| FbaI | TGATCA | 1 cut(s) | 103 |
| FblI | GTMKAC | 1 cut(s) | 1950 |
| Fnu4HI | GCNGC | 6 cut(s) | 171, 357, 546, 549, 1137, 1710 |
| FokI | GGATG | 4 cut(s) | 503, 1262, 1568, 1661 |
| FriOI | GRGCYC | 3 cut(s) | 796, 933, 1401 |
| Fsp4HI | GCNGC | 6 cut(s) | 171, 357, 546, 549, 1137, 1710 |
| FspBI | CTAG | 1 cut(s) | 918 |
| GluI | GCNGC | 6 cut(s) | 171, 357, 546, 549, 1137, 1710 |
| GsuI | CTGGAG | 2 cut(s) | 1052, 1398 |
| HaeIII | GGCC | 1 cut(s) | 280 |
| HapII | CCGG | 8 cut(s) | 297, 780, 1236, 1326, 1404, 1503, 1706, 1872 |
| HgaI | GACGC | 4 cut(s) | 49, 176, 224, 464 |
| Hin1II | CATG | 7 cut(s) | 193, 271, 379, 940, 1016, 1249, 1666 |
| HincII | GTYRAC | 3 cut(s) | 202, 733, 1951 |
| HindII | GTYRAC | 3 cut(s) | 202, 733, 1951 |
| HinfI | GANTC | 9 cut(s) | 146, 178, 289, 467, 525, 835, 872, 1733, 1750 |
| HpaI | GTTAAC | 2 cut(s) | 202, 733 |
| HpaII | CCGG | 8 cut(s) | 297, 780, 1236, 1326, 1404, 1503, 1706, 1872 |
| Hpy166II | GTNNAC | 4 cut(s) | 202, 733, 1345, 1951 |
| Hpy188I | TCNGA | 7 cut(s) | 65, 151, 247, 261, 855, 1315, 1600 |
| Hpy8I | GTNNAC | 4 cut(s) | 202, 733, 1345, 1951 |
| Hpy99I | CGWCG | 4 cut(s) | 65, 218, 240, 329 |
| HpyCH4III | ACNGT | 7 cut(s) | 83, 208, 448, 563, 1009, 1133, 1444 |
| HpyCH4IV | ACGT | 3 cut(s) | 132, 324, 516 |
| HpyCH4V | TGCA | 3 cut(s) | 1098, 1712, 1827 |
| HpyF10VI | GCNNNNNNNGC | 5 cut(s) | 302, 554, 1133, 1637, 1913 |
| HpySE526I | ACGT | 3 cut(s) | 132, 324, 516 |
| Hsp92II | CATG | 7 cut(s) | 193, 271, 379, 940, 1016, 1249, 1666 |
| Kpn2I | TCCGGA | 1 cut(s) | 1403 |
| KpnI | GGTACC | 1 cut(s) | 48 |
| Ksp22I | TGATCA | 1 cut(s) | 103 |
| KspAI | GTTAAC | 2 cut(s) | 202, 733 |
| LmnI | GCTCC | 9 cut(s) | 928, 961, 1071, 1148, 1423, 1609, 1707, 1856, 1912 |
| Lsp1109I | GCAGC | 4 cut(s) | 182, 368, 1123, 1696 |
| LweI | GCATC | 6 cut(s) | 381, 481, 976, 1450, 1830, 1894 |
| MaeI | CTAG | 1 cut(s) | 918 |
| MaeII | ACGT | 3 cut(s) | 132, 324, 516 |
| MaeIII | GTNAC | 8 cut(s) | 164, 263, 421, 442, 689, 782, 1204, 1766 |
| MfeI | CAATTG | 1 cut(s) | 1061 |
| MflI | RGATCY | 2 cut(s) | 940, 1186 |
| MhlI | GDGCHC | 5 cut(s) | 796, 933, 1007, 1401, 1614 |
| MlsI | TGGCCA | 1 cut(s) | 280 |
| MluCI | AATT | 5 cut(s) | 272, 1061, 1391, 1407, 1811 |
| MluNI | TGGCCA | 1 cut(s) | 280 |
| MlyI | GAGTC | 4 cut(s) | 140, 461, 829, 866 |
| MmeI | TCCRAC | 2 cut(s) | 43, 568 |
| Mox20I | TGGCCA | 1 cut(s) | 280 |
| MroI | TCCGGA | 1 cut(s) | 1403 |
| MroXI | GAANNNNTTC | 1 cut(s) | 350 |
| MscI | TGGCCA | 1 cut(s) | 280 |
| MseI | TTAA | 4 cut(s) | 201, 387, 732, 1107 |
| MslI | CAYNNNNRTG | 2 cut(s) | 81, 702 |
| Msp20I | TGGCCA | 1 cut(s) | 280 |
| MspA1I | CMGCKG | 2 cut(s) | 359, 1161 |
| MspI | CCGG | 8 cut(s) | 297, 780, 1236, 1326, 1404, 1503, 1706, 1872 |
| MspR9I | CCNGG | 6 cut(s) | 1070, 1145, 1326, 1340, 1706, 1881 |
| MunI | CAATTG | 1 cut(s) | 1061 |
| MvaI | CCWGG | 4 cut(s) | 1070, 1145, 1340, 1881 |
| MwoI | GCNNNNNNNGC | 5 cut(s) | 302, 554, 1133, 1637, 1913 |
| NciI | CCSGG | 2 cut(s) | 1326, 1706 |
| NcoI | CCATGG | 1 cut(s) | 375 |
| NlaIII | CATG | 7 cut(s) | 193, 271, 379, 940, 1016, 1249, 1666 |
| NlaIV | GGNNCC | 3 cut(s) | 46, 963, 1885 |
| NmuCI | GTSAC | 5 cut(s) | 164, 263, 421, 689, 1204 |
| NspV | TTCGAA | 1 cut(s) | 1794 |
| OliI | CACNNNNGTG | 1 cut(s) | 81 |
| PaeR7I | CTCGAG | 2 cut(s) | 1643, 1760 |
| PagI | TCATGA | 2 cut(s) | 1012, 1245 |
| PcsI | WCGNNNNNNNCGW | 2 cut(s) | 888, 1947 |
| PdmI | GAANNNNTTC | 1 cut(s) | 350 |
| PfeI | GAWTC | 5 cut(s) | 178, 289, 525, 1733, 1750 |
| PfoI | TCCNGGA | 1 cut(s) | 1068 |
| PinAI | ACCGGT | 1 cut(s) | 779 |
| PkrI | GCNGC | 6 cut(s) | 172, 358, 547, 550, 1138, 1711 |
| PleI | GAGTC | 4 cut(s) | 140, 461, 829, 866 |
| PpsI | GAGTC | 4 cut(s) | 140, 461, 829, 866 |
| PshAI | GACNNNNGTC | 1 cut(s) | 1539 |
| Psp124BI | GAGCTC | 3 cut(s) | 796, 933, 1401 |
| Psp1406I | AACGTT | 1 cut(s) | 516 |
| Psp6I | CCWGG | 4 cut(s) | 1068, 1143, 1338, 1879 |
| PspEI | GGTNACC | 2 cut(s) | 782, 1766 |
| PspGI | CCWGG | 4 cut(s) | 1068, 1143, 1338, 1879 |
| PspN4I | GGNNCC | 3 cut(s) | 46, 963, 1885 |
| PspPI | GGNCC | 4 cut(s) | 20, 299, 1877, 1931 |
| PspXI | VCTCGAGB | 1 cut(s) | 1760 |
| PsrI | GAACNNNNNNTAC | 2 cut(s) | 1640, 1672 |
| PstI | CTGCAG | 1 cut(s) | 1714 |
| PsuI | RGATCY | 2 cut(s) | 940, 1186 |
| PvuII | CAGCTG | 1 cut(s) | 359 |
| RsaI | GTAC | 4 cut(s) | 46, 1346, 1592, 1850 |
| RsaNI | GTAC | 4 cut(s) | 45, 1345, 1591, 1849 |
| RseI | CAYNNNNRTG | 2 cut(s) | 81, 702 |
| SacI | GAGCTC | 3 cut(s) | 796, 933, 1401 |
| SalI | GTCGAC | 1 cut(s) | 1949 |
| SaqAI | TTAA | 4 cut(s) | 201, 387, 732, 1107 |
| SatI | GCNGC | 6 cut(s) | 171, 357, 546, 549, 1137, 1710 |
| Sau96I | GGNCC | 4 cut(s) | 20, 299, 1877, 1931 |
| SchI | GAGTC | 4 cut(s) | 140, 461, 829, 866 |
| ScrFI | CCNGG | 6 cut(s) | 1070, 1145, 1326, 1340, 1706, 1881 |
| SduI | GDGCHC | 5 cut(s) | 796, 933, 1007, 1401, 1614 |
| SfaNI | GCATC | 6 cut(s) | 381, 481, 976, 1450, 1830, 1894 |
| SfcI | CTRYAG | 2 cut(s) | 540, 1710 |
| Sfr274I | CTCGAG | 2 cut(s) | 1643, 1760 |
| SfuI | TTCGAA | 1 cut(s) | 1794 |
| SinI | GGWCC | 4 cut(s) | 20, 299, 1877, 1931 |
| SlaI | CTCGAG | 2 cut(s) | 1643, 1760 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 81, 702 |
| SmlI | CTYRAG | 2 cut(s) | 1643, 1760 |
| SmoI | CTYRAG | 2 cut(s) | 1643, 1760 |
| Sse9I | AATT | 5 cut(s) | 272, 1061, 1391, 1407, 1811 |
| SsiI | CCGC | 4 cut(s) | 546, 549, 1161, 1923 |
| SspMI | CTAG | 1 cut(s) | 918 |
| SstI | GAGCTC | 3 cut(s) | 796, 933, 1401 |
| StyD4I | CCNGG | 6 cut(s) | 1068, 1143, 1324, 1338, 1704, 1879 |
| StyI | CCWWGG | 7 cut(s) | 375, 415, 1219, 1262, 1368, 1551, 1852 |
| TaaI | ACNGT | 7 cut(s) | 83, 208, 448, 563, 1009, 1133, 1444 |
| TaiI | ACGT | 3 cut(s) | 135, 327, 519 |
| TaqII | GACCGA | 1 cut(s) | 221 |
| TasI | AATT | 5 cut(s) | 272, 1061, 1391, 1407, 1811 |
| TatI | WGTACW | 1 cut(s) | 1590 |
| TauI | GCSGC | 2 cut(s) | 548, 551 |
| TfiI | GAWTC | 5 cut(s) | 178, 289, 525, 1733, 1750 |
| Tru1I | TTAA | 4 cut(s) | 201, 387, 732, 1107 |
| Tru9I | TTAA | 4 cut(s) | 201, 387, 732, 1107 |
| TscAI | CASTG | 2 cut(s) | 853, 1012 |
| TseFI | GTSAC | 5 cut(s) | 164, 263, 421, 689, 1204 |
| TseI | GCWGC | 4 cut(s) | 170, 356, 1136, 1709 |
| Tsp45I | GTSAC | 5 cut(s) | 164, 263, 421, 689, 1204 |
| TspDTI | ATGAA | 4 cut(s) | 206, 284, 1001, 1820 |
| TspRI | CASTG | 2 cut(s) | 853, 1012 |
| VpaK11BI | GGWCC | 4 cut(s) | 20, 299, 1877, 1931 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 1917 |
| XapI | RAATTY | 2 cut(s) | 1391, 1407 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 1859 |
| XhoI | CTCGAG | 2 cut(s) | 1643, 1760 |
| XmiI | GTMKAC | 1 cut(s) | 1950 |
| XmnI | GAANNNNTTC | 1 cut(s) | 350 |
| XspI | CTAG | 1 cut(s) | 918 |