Heat shock cognate 70 kDa
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 446 |
| AarI | CACCTGC | 1 cut(s) | 488 |
| Acc36I | ACCTGC | 2 cut(s) | 488, 1429 |
| AccB1I | GGYRCC | 1 cut(s) | 1136 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 35 |
| AccBSI | CCGCTC | 2 cut(s) | 1164, 1576 |
| AccI | GTMKAC | 1 cut(s) | 1656 |
| AccIII | TCCGGA | 1 cut(s) | 1114 |
| AciI | CCGC | 5 cut(s) | 177, 1035, 1164, 1576, 1742 |
| AclI | AACGTT | 1 cut(s) | 603 |
| AclWI | GGATC | 5 cut(s) | 951, 1258, 1408, 1421, 1788 |
| AcoI | YGGCCR | 1 cut(s) | 85 |
| AcsI | RAATTY | 3 cut(s) | 923, 1047, 1476 |
| AcuI | CTGAAG | 1 cut(s) | 458 |
| AfaI | GTAC | 9 cut(s) | 52, 631, 1059, 1146, 1188, 1231, 1279, 1352, 1601 |
| AfeI | AGCGCT | 1 cut(s) | 230 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 35, 1561 |
| AflII | CTTAAG | 1 cut(s) | 263 |
| AflIII | ACRYGT | 1 cut(s) | 1331 |
| AjnI | CCWGG | 2 cut(s) | 111, 939 |
| AleI | CACNNNNGTG | 1 cut(s) | 87 |
| AloI | GAACNNNNNNTCC | 2 cut(s) | 588, 620 |
| AluBI | AGCT | 8 cut(s) | 548, 683, 1151, 1304, 1439, 1699, 1739, 1825 |
| AluI | AGCT | 8 cut(s) | 548, 683, 1151, 1304, 1439, 1699, 1739, 1825 |
| Alw21I | GWGCWC | 1 cut(s) | 1701 |
| Alw26I | GTCTC | 1 cut(s) | 1371 |
| AlwI | GGATC | 5 cut(s) | 951, 1258, 1408, 1421, 1788 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 551 |
| Ama87I | CYCGRG | 1 cut(s) | 1374 |
| Aor13HI | TCCGGA | 1 cut(s) | 1114 |
| Aor51HI | AGCGCT | 1 cut(s) | 230 |
| AoxI | GGCC | 3 cut(s) | 85, 284, 1638 |
| ApeKI | GCWGC | 5 cut(s) | 548, 551, 1151, 1424, 1739 |
| ApoI | RAATTY | 3 cut(s) | 923, 1047, 1476 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 841 |
| AspLEI | GCGC | 1 cut(s) | 231 |
| AspS9I | GGNCC | 1 cut(s) | 26 |
| AsuHPI | GGTGA | 6 cut(s) | 182, 688, 722, 745, 1178, 1370 |
| AvaI | CYCGRG | 1 cut(s) | 1374 |
| AvaII | GGWCC | 1 cut(s) | 26 |
| BaeGI | GKGCMC | 1 cut(s) | 1722 |
| BamHI | GGATCC | 1 cut(s) | 1413 |
| BanI | GGYRCC | 1 cut(s) | 1136 |
| BanII | GRGCYC | 2 cut(s) | 1375, 1701 |
| BauI | CACGAG | 1 cut(s) | 386 |
| Bbv12I | GWGCWC | 1 cut(s) | 1701 |
| BbvCI | CCTCAGC | 1 cut(s) | 1494 |
| BbvI | GCAGC | 5 cut(s) | 535, 538, 1138, 1411, 1726 |
| BccI | CCATC | 4 cut(s) | 139, 587, 1316, 1466 |
| BceAI | ACGGC | 2 cut(s) | 72, 100 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 1444, 1478 |
| BciT130I | CCWGG | 2 cut(s) | 113, 941 |
| BciVI | GTATCC | 2 cut(s) | 539, 1387 |
| BcoDI | GTCTC | 1 cut(s) | 1371 |
| BfaI | CTAG | 3 cut(s) | 156, 1044, 1428 |
| BfoI | RGCGCY | 1 cut(s) | 232 |
| BfrI | CTTAAG | 1 cut(s) | 263 |
| BfuAI | ACCTGC | 2 cut(s) | 488, 1429 |
| BfuI | GTATCC | 2 cut(s) | 539, 1387 |
| BglII | AGATCT | 1 cut(s) | 1705 |
| BisI | GCNGC | 7 cut(s) | 177, 549, 552, 1036, 1152, 1425, 1740 |
| BlsI | GCNGC | 7 cut(s) | 178, 550, 553, 1037, 1153, 1426, 1741 |
| Bme1390I | CCNGG | 2 cut(s) | 113, 941 |
| Bme18I | GGWCC | 1 cut(s) | 26 |
| BmeT110I | CYCGRG | 1 cut(s) | 1374 |
| BmgT120I | GGNCC | 1 cut(s) | 26 |
| BmiI | GGNNCC | 4 cut(s) | 27, 966, 1138, 1415 |
| BmrFI | CCNGG | 2 cut(s) | 113, 941 |
| BmsI | GCATC | 8 cut(s) | 163, 211, 244, 384, 484, 979, 1116, 1572 |
| Bpu10I | CCTNAGC | 1 cut(s) | 1494 |
| Bsa29I | ATCGAT | 1 cut(s) | 1464 |
| BsaBI | GATNNNNATC | 2 cut(s) | 38, 699 |
| BsaJI | CCNNGG | 4 cut(s) | 46, 112, 363, 1650 |
| BsaWI | WCCGGW | 1 cut(s) | 1114 |
| BsaXI | ACNNNNNCTCC | 4 cut(s) | 176, 206, 1628, 1658 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 35, 1561 |
| Bse3DI | GCAATG | 2 cut(s) | 555, 585 |
| Bse8I | GATNNNNATC | 2 cut(s) | 38, 699 |
| BseAI | TCCGGA | 1 cut(s) | 1114 |
| BseBI | CCWGG | 2 cut(s) | 113, 941 |
| BseCI | ATCGAT | 1 cut(s) | 1464 |
| BseDI | CCNNGG | 4 cut(s) | 46, 112, 363, 1650 |
| BseGI | GGATG | 5 cut(s) | 499, 994, 1123, 1213, 1584 |
| BseJI | GATNNNNATC | 2 cut(s) | 38, 699 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 35, 1561 |
| BseMI | GCAATG | 2 cut(s) | 555, 585 |
| BseMII | CTCAG | 4 cut(s) | 664, 1454, 1485, 1723 |
| BseRI | GAGGAG | 5 cut(s) | 380, 817, 944, 1580, 1631 |
| BseSI | GKGCMC | 1 cut(s) | 1722 |
| BseX3I | CGGCCG | 1 cut(s) | 85 |
| BseXI | GCAGC | 5 cut(s) | 535, 538, 1138, 1411, 1726 |
| BseYI | CCCAGC | 1 cut(s) | 454 |
| Bsh1285I | CGRYCG | 1 cut(s) | 88 |
| BshFI | GGCC | 3 cut(s) | 87, 286, 1640 |
| BshNI | GGYRCC | 1 cut(s) | 1136 |
| BshVI | ATCGAT | 1 cut(s) | 1464 |
| BsiEI | CGRYCG | 1 cut(s) | 88 |
| BsiHKAI | GWGCWC | 1 cut(s) | 1701 |
| BsiHKCI | CYCGRG | 1 cut(s) | 1374 |
| BsiSI | CCGG | 2 cut(s) | 690, 1115 |
| BslFI | GGGAC | 1 cut(s) | 437 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 35, 1561 |
| BsmAI | GTCTC | 1 cut(s) | 1371 |
| BsmFI | GGGAC | 1 cut(s) | 437 |
| BsnI | GGCC | 3 cut(s) | 87, 286, 1640 |
| BsoBI | CYCGRG | 1 cut(s) | 1374 |
| Bsp1286I | GDGCHC | 3 cut(s) | 1375, 1701, 1722 |
| Bsp13I | TCCGGA | 1 cut(s) | 1114 |
| Bsp1407I | TGTACA | 1 cut(s) | 1144 |
| BspACI | CCGC | 5 cut(s) | 177, 1035, 1164, 1576, 1742 |
| BspANI | GGCC | 3 cut(s) | 87, 286, 1640 |
| BspCNI | CTCAG | 4 cut(s) | 663, 1453, 1486, 1722 |
| BspDI | ATCGAT | 1 cut(s) | 1464 |
| BspEI | TCCGGA | 1 cut(s) | 1114 |
| BspLI | GGNNCC | 4 cut(s) | 27, 966, 1138, 1415 |
| BspMI | ACCTGC | 2 cut(s) | 488, 1429 |
| BspPI | GGATC | 5 cut(s) | 951, 1258, 1408, 1421, 1788 |
| BspT107I | GGYRCC | 1 cut(s) | 1136 |
| BspTI | CTTAAG | 1 cut(s) | 263 |
| BsrBI | CCGCTC | 2 cut(s) | 1164, 1576 |
| BsrDI | GCAATG | 2 cut(s) | 555, 585 |
| BsrGI | TGTACA | 1 cut(s) | 1144 |
| BssECI | CCNNGG | 4 cut(s) | 46, 112, 363, 1650 |
| BssSI | CACGAG | 1 cut(s) | 386 |
| BssT1I | CCWWGG | 2 cut(s) | 46, 1650 |
| Bst2BI | CACGAG | 1 cut(s) | 386 |
| Bst2UI | CCWGG | 2 cut(s) | 113, 941 |
| Bst4CI | ACNGT | 6 cut(s) | 214, 451, 853, 1277, 1315, 1450 |
| Bst6I | CTCTTC | 3 cut(s) | 339, 654, 1764 |
| BstAFI | CTTAAG | 1 cut(s) | 263 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 1136 |
| BstAUI | TGTACA | 1 cut(s) | 1144 |
| BstC8I | GCNNGC | 1 cut(s) | 511 |
| BstDEI | CTNAG | 5 cut(s) | 650, 1440, 1494, 1560, 1709 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 1559 |
| BstF5I | GGATG | 5 cut(s) | 499, 994, 1123, 1213, 1584 |
| BstH2I | RGCGCY | 1 cut(s) | 232 |
| BstHHI | GCGC | 1 cut(s) | 231 |
| BstMAI | GTCTC | 1 cut(s) | 1371 |
| BstMCI | CGRYCG | 1 cut(s) | 88 |
| BstMWI | GCNNNNNNNGC | 6 cut(s) | 11, 557, 1136, 1170, 1582, 1646 |
| BstNI | CCWGG | 2 cut(s) | 113, 941 |
| BstNSI | RCATGY | 2 cut(s) | 1335, 1684 |
| BstSCI | CCNGG | 2 cut(s) | 111, 939 |
| BstSLI | GKGCMC | 1 cut(s) | 1722 |
| BstV1I | GCAGC | 5 cut(s) | 535, 538, 1138, 1411, 1726 |
| BstX2I | RGATCY | 3 cut(s) | 943, 1413, 1705 |
| BstYI | RGATCY | 3 cut(s) | 943, 1413, 1705 |
| BstZI | CGGCCG | 1 cut(s) | 85 |
| Bsu15I | ATCGAT | 1 cut(s) | 1464 |
| BsuI | GTATCC | 2 cut(s) | 539, 1387 |
| BsuRI | GGCC | 3 cut(s) | 87, 286, 1640 |
| BsuTUI | ATCGAT | 1 cut(s) | 1464 |
| BtsCI | GGATG | 5 cut(s) | 499, 994, 1123, 1213, 1584 |
| BtsI | GCAGTG | 1 cut(s) | 414 |
| BtsIMutI | CAGTG | 2 cut(s) | 210, 414 |
| BveI | ACCTGC | 2 cut(s) | 488, 1429 |
| Cac8I | GCNNGC | 1 cut(s) | 511 |
| CaiI | CAGNNNCTG | 1 cut(s) | 551 |
| CfoI | GCGC | 1 cut(s) | 231 |
| Cfr13I | GGNCC | 1 cut(s) | 26 |
| ClaI | ATCGAT | 1 cut(s) | 1464 |
| Csp6I | GTAC | 9 cut(s) | 51, 630, 1058, 1145, 1187, 1230, 1278, 1351, 1600 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 306, 341 |
| CviAII | CATG | 7 cut(s) | 63, 196, 274, 514, 1332, 1456, 1681 |
| CviQI | GTAC | 9 cut(s) | 51, 630, 1058, 1145, 1187, 1230, 1278, 1351, 1600 |
| DdeI | CTNAG | 5 cut(s) | 650, 1440, 1494, 1560, 1709 |
| DraI | TTTAAA | 1 cut(s) | 952 |
| EaeI | YGGCCR | 1 cut(s) | 85 |
| EagI | CGGCCG | 1 cut(s) | 85 |
| Eam1104I | CTCTTC | 3 cut(s) | 339, 654, 1764 |
| EarI | CTCTTC | 3 cut(s) | 339, 654, 1764 |
| Ecl136II | GAGCTC | 1 cut(s) | 1699 |
| EclXI | CGGCCG | 1 cut(s) | 85 |
| Eco130I | CCWWGG | 2 cut(s) | 46, 1650 |
| Eco24I | GRGCYC | 2 cut(s) | 1375, 1701 |
| Eco32I | GATATC | 1 cut(s) | 372 |
| Eco47I | GGWCC | 1 cut(s) | 26 |
| Eco47III | AGCGCT | 1 cut(s) | 230 |
| Eco52I | CGGCCG | 1 cut(s) | 85 |
| Eco53kI | GAGCTC | 1 cut(s) | 1699 |
| Eco57I | CTGAAG | 1 cut(s) | 458 |
| Eco88I | CYCGRG | 1 cut(s) | 1374 |
| EcoICRI | GAGCTC | 1 cut(s) | 1699 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 1559 |
| EcoRI | GAATTC | 2 cut(s) | 1047, 1476 |
| EcoRII | CCWGG | 2 cut(s) | 111, 939 |
| EcoRV | GATATC | 1 cut(s) | 372 |
| EcoT14I | CCWWGG | 2 cut(s) | 46, 1650 |
| EcoT38I | GRGCYC | 2 cut(s) | 1375, 1701 |
| ErhI | CCWWGG | 2 cut(s) | 46, 1650 |
| FaeI | CATG | 7 cut(s) | 66, 199, 277, 517, 1335, 1459, 1684 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 1634, 1666 |
| FaqI | GGGAC | 1 cut(s) | 437 |
| FatI | CATG | 7 cut(s) | 62, 195, 273, 513, 1331, 1455, 1680 |
| FauNDI | CATATG | 1 cut(s) | 1132 |
| FblI | GTMKAC | 1 cut(s) | 1656 |
| Fnu4HI | GCNGC | 7 cut(s) | 177, 549, 552, 1036, 1152, 1425, 1740 |
| FokI | GGATG | 5 cut(s) | 506, 1001, 1130, 1220, 1591 |
| FriOI | GRGCYC | 2 cut(s) | 1375, 1701 |
| Fsp4HI | GCNGC | 7 cut(s) | 177, 549, 552, 1036, 1152, 1425, 1740 |
| FspBI | CTAG | 3 cut(s) | 156, 1044, 1428 |
| GlaI | GCGC | 1 cut(s) | 230 |
| GluI | GCNGC | 7 cut(s) | 177, 549, 552, 1036, 1152, 1425, 1740 |
| GsaI | CCCAGC | 1 cut(s) | 458 |
| HaeII | RGCGCY | 1 cut(s) | 232 |
| HaeIII | GGCC | 3 cut(s) | 87, 286, 1640 |
| HapII | CCGG | 2 cut(s) | 690, 1115 |
| HhaI | GCGC | 1 cut(s) | 231 |
| Hin1II | CATG | 7 cut(s) | 66, 199, 277, 517, 1335, 1459, 1684 |
| Hin6I | GCGC | 1 cut(s) | 229 |
| HinP1I | GCGC | 1 cut(s) | 229 |
| HincII | GTYRAC | 2 cut(s) | 331, 1657 |
| HindII | GTYRAC | 2 cut(s) | 331, 1657 |
| HindIII | AAGCTT | 1 cut(s) | 1823 |
| HinfI | GANTC | 5 cut(s) | 246, 581, 875, 1466, 1774 |
| HpaII | CCGG | 2 cut(s) | 690, 1115 |
| HphI | GGTGA | 6 cut(s) | 182, 688, 722, 745, 1178, 1370 |
| Hpy166II | GTNNAC | 5 cut(s) | 331, 357, 736, 1351, 1657 |
| Hpy188I | TCNGA | 5 cut(s) | 422, 653, 1443, 1471, 1693 |
| Hpy188III | TCNNGA | 4 cut(s) | 388, 569, 1115, 1778 |
| Hpy8I | GTNNAC | 5 cut(s) | 331, 357, 736, 1351, 1657 |
| HpyAV | CCTTC | 7 cut(s) | 482, 656, 979, 1163, 1177, 1249, 1403 |
| HpyCH4III | ACNGT | 6 cut(s) | 214, 451, 853, 1277, 1315, 1450 |
| HpyCH4IV | ACGT | 3 cut(s) | 160, 603, 815 |
| HpyF10VI | GCNNNNNNNGC | 6 cut(s) | 11, 557, 1136, 1170, 1582, 1646 |
| HpyF3I | CTNAG | 5 cut(s) | 650, 1440, 1494, 1560, 1709 |
| HpySE526I | ACGT | 3 cut(s) | 160, 603, 815 |
| Hsp92II | CATG | 7 cut(s) | 66, 199, 277, 517, 1335, 1459, 1684 |
| HspAI | GCGC | 1 cut(s) | 229 |
| Kpn2I | TCCGGA | 1 cut(s) | 1114 |
| LmnI | GCTCC | 1 cut(s) | 1704 |
| Lsp1109I | GCAGC | 5 cut(s) | 535, 538, 1138, 1411, 1726 |
| LweI | GCATC | 8 cut(s) | 163, 211, 244, 384, 484, 979, 1116, 1572 |
| MaeI | CTAG | 3 cut(s) | 156, 1044, 1428 |
| MaeII | ACGT | 3 cut(s) | 160, 603, 815 |
| MaeIII | GTNAC | 2 cut(s) | 269, 1210 |
| MbiI | CCGCTC | 2 cut(s) | 1164, 1576 |
| MflI | RGATCY | 3 cut(s) | 943, 1413, 1705 |
| MhlI | GDGCHC | 3 cut(s) | 1375, 1701, 1722 |
| MmeI | TCCRAC | 3 cut(s) | 1185, 1371, 1441 |
| MroI | TCCGGA | 1 cut(s) | 1114 |
| MroXI | GAANNNNTTC | 1 cut(s) | 841 |
| MseI | TTAA | 6 cut(s) | 164, 264, 465, 951, 1083, 1674 |
| MslI | CAYNNNNRTG | 1 cut(s) | 87 |
| MspA1I | CMGCKG | 1 cut(s) | 548 |
| MspCI | CTTAAG | 1 cut(s) | 263 |
| MspI | CCGG | 2 cut(s) | 690, 1115 |
| MspR9I | CCNGG | 2 cut(s) | 113, 941 |
| MvaI | CCWGG | 2 cut(s) | 113, 941 |
| MwoI | GCNNNNNNNGC | 6 cut(s) | 11, 557, 1136, 1170, 1582, 1646 |
| NdeI | CATATG | 1 cut(s) | 1132 |
| NlaIII | CATG | 7 cut(s) | 66, 199, 277, 517, 1335, 1459, 1684 |
| NlaIV | GGNNCC | 4 cut(s) | 27, 966, 1138, 1415 |
| NmeAIII | GCCGAG | 1 cut(s) | 388 |
| NmuCI | GTSAC | 2 cut(s) | 269, 1210 |
| NspI | RCATGY | 2 cut(s) | 1335, 1684 |
| OliI | CACNNNNGTG | 1 cut(s) | 87 |
| PaqCI | CACCTGC | 1 cut(s) | 488 |
| PciI | ACATGT | 1 cut(s) | 1331 |
| PdmI | GAANNNNTTC | 1 cut(s) | 841 |
| PfeI | GAWTC | 5 cut(s) | 246, 581, 875, 1466, 1774 |
| PflFI | GACNNNGTC | 1 cut(s) | 1196 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 35 |
| PkrI | GCNGC | 7 cut(s) | 178, 550, 553, 1037, 1153, 1426, 1741 |
| PscI | ACATGT | 1 cut(s) | 1331 |
| PsiI | TTATAA | 1 cut(s) | 446 |
| Psp124BI | GAGCTC | 1 cut(s) | 1701 |
| Psp1406I | AACGTT | 1 cut(s) | 603 |
| Psp6I | CCWGG | 2 cut(s) | 111, 939 |
| PspFI | CCCAGC | 1 cut(s) | 454 |
| PspGI | CCWGG | 2 cut(s) | 111, 939 |
| PspN4I | GGNNCC | 4 cut(s) | 27, 966, 1138, 1415 |
| PspPI | GGNCC | 1 cut(s) | 26 |
| PstNI | CAGNNNCTG | 1 cut(s) | 551 |
| PsuI | RGATCY | 3 cut(s) | 943, 1413, 1705 |
| PsyI | GACNNNGTC | 1 cut(s) | 1196 |
| PvuII | CAGCTG | 1 cut(s) | 548 |
| RsaI | GTAC | 9 cut(s) | 52, 631, 1059, 1146, 1188, 1231, 1279, 1352, 1601 |
| RsaNI | GTAC | 9 cut(s) | 51, 630, 1058, 1145, 1187, 1230, 1278, 1351, 1600 |
| RseI | CAYNNNNRTG | 1 cut(s) | 87 |
| SacI | GAGCTC | 1 cut(s) | 1701 |
| SalI | GTCGAC | 1 cut(s) | 1655 |
| SaqAI | TTAA | 6 cut(s) | 164, 264, 465, 951, 1083, 1674 |
| SatI | GCNGC | 7 cut(s) | 177, 549, 552, 1036, 1152, 1425, 1740 |
| Sau96I | GGNCC | 1 cut(s) | 26 |
| ScrFI | CCNGG | 2 cut(s) | 113, 941 |
| SduI | GDGCHC | 3 cut(s) | 1375, 1701, 1722 |
| SfaNI | GCATC | 8 cut(s) | 163, 211, 244, 384, 484, 979, 1116, 1572 |
| SinI | GGWCC | 1 cut(s) | 26 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 87 |
| SmlI | CTYRAG | 1 cut(s) | 263 |
| SmoI | CTYRAG | 1 cut(s) | 263 |
| SsiI | CCGC | 5 cut(s) | 177, 1035, 1164, 1576, 1742 |
| SspI | AATATT | 1 cut(s) | 1177 |
| SspMI | CTAG | 3 cut(s) | 156, 1044, 1428 |
| SstI | GAGCTC | 1 cut(s) | 1701 |
| StyD4I | CCNGG | 2 cut(s) | 111, 939 |
| StyI | CCWWGG | 2 cut(s) | 46, 1650 |
| TaaI | ACNGT | 6 cut(s) | 214, 451, 853, 1277, 1315, 1450 |
| TaiI | ACGT | 3 cut(s) | 163, 606, 818 |
| TatI | WGTACW | 2 cut(s) | 1144, 1599 |
| TauI | GCSGC | 2 cut(s) | 179, 1038 |
| TfiI | GAWTC | 5 cut(s) | 246, 581, 875, 1466, 1774 |
| Tru1I | TTAA | 6 cut(s) | 164, 264, 465, 951, 1083, 1674 |
| Tru9I | TTAA | 6 cut(s) | 164, 264, 465, 951, 1083, 1674 |
| TscAI | CASTG | 2 cut(s) | 217, 414 |
| TseFI | GTSAC | 2 cut(s) | 269, 1210 |
| TseI | GCWGC | 5 cut(s) | 548, 551, 1151, 1424, 1739 |
| Tsp45I | GTSAC | 2 cut(s) | 269, 1210 |
| TspDTI | ATGAA | 8 cut(s) | 51, 212, 357, 530, 890, 1134, 1268, 1679 |
| TspRI | CASTG | 2 cut(s) | 217, 414 |
| Tth111I | GACNNNGTC | 1 cut(s) | 1196 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 35 |
| Vha464I | CTTAAG | 1 cut(s) | 263 |
| VpaK11BI | GGWCC | 1 cut(s) | 26 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 1559 |
| XapI | RAATTY | 3 cut(s) | 923, 1047, 1476 |
| XceI | RCATGY | 2 cut(s) | 1335, 1684 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 1648 |
| XmiI | GTMKAC | 1 cut(s) | 1656 |
| XmnI | GAANNNNTTC | 1 cut(s) | 841 |
| XspI | CTAG | 3 cut(s) | 156, 1044, 1428 |