Belongs to the heat shock protein 70 family
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 2 cut(s) | 14, 1467 |
| AasI | GACNNNNNNGTC | 2 cut(s) | 1021, 1418 |
| Acc36I | ACCTGC | 2 cut(s) | 268, 1093 |
| AccB1I | GGYRCC | 1 cut(s) | 941 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 1724 |
| AccIII | TCCGGA | 1 cut(s) | 1211 |
| AciI | CCGC | 4 cut(s) | 31, 167, 945, 1391 |
| AclWI | GGATC | 3 cut(s) | 756, 850, 1138 |
| AcsI | RAATTY | 1 cut(s) | 1444 |
| AcuI | CTGAAG | 1 cut(s) | 1614 |
| AcyI | GRCGYC | 1 cut(s) | 942 |
| AfaI | GTAC | 4 cut(s) | 258, 951, 1239, 1400 |
| AfiI | CCNNNNNNNGG | 3 cut(s) | 1682, 1721, 1724 |
| AflIII | ACRYGT | 1 cut(s) | 1287 |
| AjnI | CCWGG | 3 cut(s) | 109, 1146, 1449 |
| AluBI | AGCT | 8 cut(s) | 82, 602, 623, 739, 1207, 1510, 1582, 1621 |
| AluI | AGCT | 8 cut(s) | 82, 602, 623, 739, 1207, 1510, 1582, 1621 |
| Alw21I | GWGCWC | 5 cut(s) | 330, 604, 815, 1065, 1623 |
| Alw26I | GTCTC | 1 cut(s) | 451 |
| AlwI | GGATC | 3 cut(s) | 756, 850, 1138 |
| Aor13HI | TCCGGA | 1 cut(s) | 1211 |
| AoxI | GGCC | 4 cut(s) | 86, 106, 316, 1312 |
| ApeKI | GCWGC | 7 cut(s) | 164, 353, 356, 865, 956, 1517, 1670 |
| ApoI | RAATTY | 1 cut(s) | 1444 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 671, 703 |
| AspLEI | GCGC | 2 cut(s) | 944, 1670 |
| AspS9I | GGNCC | 3 cut(s) | 107, 994, 1739 |
| AsuC2I | CCSGG | 1 cut(s) | 1134 |
| AsuHPI | GGTGA | 7 cut(s) | 125, 493, 527, 550, 709, 983, 1172 |
| AvaII | GGWCC | 2 cut(s) | 994, 1739 |
| BanI | GGYRCC | 1 cut(s) | 941 |
| BanII | GRGCYC | 2 cut(s) | 604, 1623 |
| BbsI | GAAGAC | 2 cut(s) | 1357, 1416 |
| Bbv12I | GWGCWC | 5 cut(s) | 330, 604, 815, 1065, 1623 |
| BbvCI | CCTCAGC | 2 cut(s) | 1296, 1587 |
| BbvI | GCAGC | 7 cut(s) | 151, 340, 343, 877, 943, 1529, 1657 |
| BccI | CCATC | 7 cut(s) | 529, 677, 722, 796, 1340, 1372, 1649 |
| BceAI | ACGGC | 2 cut(s) | 914, 1588 |
| BciT130I | CCWGG | 3 cut(s) | 111, 1148, 1451 |
| BcnI | CCSGG | 1 cut(s) | 1134 |
| BcoDI | GTCTC | 1 cut(s) | 451 |
| BfaI | CTAG | 2 cut(s) | 849, 1568 |
| BfmI | CTRYAG | 1 cut(s) | 1308 |
| BfoI | RGCGCY | 2 cut(s) | 945, 1671 |
| BfuAI | ACCTGC | 2 cut(s) | 268, 1093 |
| BisI | GCNGC | 8 cut(s) | 165, 354, 357, 866, 945, 957, 1518, 1671 |
| BlsI | GCNGC | 8 cut(s) | 166, 355, 358, 867, 946, 958, 1519, 1672 |
| Bme1390I | CCNGG | 4 cut(s) | 111, 1134, 1148, 1451 |
| Bme18I | GGWCC | 2 cut(s) | 994, 1739 |
| BmgT120I | GGNCC | 3 cut(s) | 107, 994, 1739 |
| BmiI | GGNNCC | 4 cut(s) | 436, 771, 943, 1741 |
| BmrFI | CCNGG | 4 cut(s) | 111, 1134, 1148, 1451 |
| BmsI | GCATC | 7 cut(s) | 13, 189, 289, 784, 1258, 1504, 1531 |
| BoxI | GACNNNNGTC | 1 cut(s) | 392 |
| BpiI | GAAGAC | 2 cut(s) | 1357, 1416 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 665, 697 |
| BpmI | CTGGAG | 3 cut(s) | 516, 1065, 1472 |
| Bpu10I | CCTNAGC | 2 cut(s) | 1296, 1587 |
| BpuMI | CCSGG | 1 cut(s) | 1134 |
| Bsa29I | ATCGAT | 1 cut(s) | 1266 |
| BsaBI | GATNNNNATC | 1 cut(s) | 1331 |
| BsaHI | GRCGYC | 1 cut(s) | 942 |
| BsaJI | CCNNGG | 6 cut(s) | 109, 1026, 1070, 1229, 1359, 1449 |
| BsaWI | WCCGGW | 1 cut(s) | 1211 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 37, 67 |
| Bsc4I | CCNNNNNNNGG | 3 cut(s) | 1682, 1721, 1724 |
| Bse118I | RCCGGY | 1 cut(s) | 104 |
| Bse1I | ACTGG | 3 cut(s) | 390, 773, 1726 |
| Bse3DI | GCAATG | 4 cut(s) | 309, 360, 985, 1644 |
| Bse8I | GATNNNNATC | 1 cut(s) | 1331 |
| BseAI | TCCGGA | 1 cut(s) | 1211 |
| BseBI | CCWGG | 3 cut(s) | 111, 1148, 1451 |
| BseCI | ATCGAT | 1 cut(s) | 1266 |
| BseDI | CCNNGG | 6 cut(s) | 109, 1026, 1070, 1229, 1359, 1449 |
| BseGI | GGATG | 5 cut(s) | 304, 540, 650, 1495, 1714 |
| BseJI | GATNNNNATC | 1 cut(s) | 1331 |
| BseLI | CCNNNNNNNGG | 3 cut(s) | 1682, 1721, 1724 |
| BseMI | GCAATG | 4 cut(s) | 309, 360, 985, 1644 |
| BseMII | CTCAG | 5 cut(s) | 293, 674, 1256, 1287, 1578 |
| BseNI | ACTGG | 3 cut(s) | 390, 773, 1726 |
| BseRI | GAGGAG | 6 cut(s) | 49, 164, 444, 1008, 1379, 1430 |
| BseXI | GCAGC | 7 cut(s) | 151, 340, 343, 877, 943, 1529, 1657 |
| BseYI | CCCAGC | 1 cut(s) | 302 |
| BsgI | GTGCAG | 3 cut(s) | 884, 1010, 1209 |
| BshFI | GGCC | 4 cut(s) | 88, 108, 318, 1314 |
| BshNI | GGYRCC | 1 cut(s) | 941 |
| BshVI | ATCGAT | 1 cut(s) | 1266 |
| BsiHKAI | GWGCWC | 5 cut(s) | 330, 604, 815, 1065, 1623 |
| BsiSI | CCGG | 3 cut(s) | 105, 1134, 1212 |
| BslFI | GGGAC | 2 cut(s) | 241, 1725 |
| BslI | CCNNNNNNNGG | 3 cut(s) | 1682, 1721, 1724 |
| BsmAI | GTCTC | 1 cut(s) | 451 |
| BsmFI | GGGAC | 2 cut(s) | 241, 1725 |
| BsmI | GAATGC | 2 cut(s) | 247, 1278 |
| BsnI | GGCC | 4 cut(s) | 88, 108, 318, 1314 |
| Bsp1286I | GDGCHC | 5 cut(s) | 330, 604, 815, 1065, 1623 |
| Bsp13I | TCCGGA | 1 cut(s) | 1211 |
| Bsp1407I | TGTACA | 1 cut(s) | 949 |
| Bsp143I | GATC | 5 cut(s) | 421, 748, 842, 1143, 1245 |
| BspACI | CCGC | 4 cut(s) | 31, 167, 945, 1391 |
| BspANI | GGCC | 4 cut(s) | 88, 108, 318, 1314 |
| BspCNI | CTCAG | 5 cut(s) | 292, 673, 1255, 1288, 1579 |
| BspDI | ATCGAT | 1 cut(s) | 1266 |
| BspEI | TCCGGA | 1 cut(s) | 1211 |
| BspLI | GGNNCC | 4 cut(s) | 436, 771, 943, 1741 |
| BspMI | ACCTGC | 2 cut(s) | 268, 1093 |
| BspPI | GGATC | 3 cut(s) | 756, 850, 1138 |
| BspQI | GCTCTTC | 1 cut(s) | 729 |
| BspT107I | GGYRCC | 1 cut(s) | 941 |
| BsrDI | GCAATG | 4 cut(s) | 309, 360, 985, 1644 |
| BsrFI | RCCGGY | 1 cut(s) | 104 |
| BsrGI | TGTACA | 1 cut(s) | 949 |
| BsrI | ACTGG | 3 cut(s) | 390, 773, 1726 |
| BssAI | RCCGGY | 1 cut(s) | 104 |
| BssECI | CCNNGG | 6 cut(s) | 109, 1026, 1070, 1229, 1359, 1449 |
| BssMI | GATC | 5 cut(s) | 421, 748, 842, 1143, 1245 |
| BssNI | GRCGYC | 1 cut(s) | 942 |
| BssT1I | CCWWGG | 4 cut(s) | 1026, 1070, 1229, 1359 |
| Bst2UI | CCWGG | 3 cut(s) | 111, 1148, 1451 |
| Bst4CI | ACNGT | 6 cut(s) | 235, 256, 817, 1060, 1252, 1356 |
| Bst6I | CTCTTC | 3 cut(s) | 456, 729, 1590 |
| BstACI | GRCGYC | 1 cut(s) | 942 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 874 |
| BstAUI | TGTACA | 1 cut(s) | 949 |
| BstC8I | GCNNGC | 5 cut(s) | 106, 288, 316, 1554, 1584 |
| BstDEI | CTNAG | 6 cut(s) | 279, 605, 660, 1242, 1296, 1587 |
| BstF5I | GGATG | 5 cut(s) | 304, 540, 650, 1495, 1714 |
| BstH2I | RGCGCY | 2 cut(s) | 945, 1671 |
| BstHHI | GCGC | 2 cut(s) | 944, 1670 |
| BstKTI | GATC | 5 cut(s) | 424, 751, 845, 1146, 1248 |
| BstMAI | GTCTC | 1 cut(s) | 451 |
| BstMBI | GATC | 5 cut(s) | 421, 748, 842, 1143, 1245 |
| BstMWI | GCNNNNNNNGC | 4 cut(s) | 362, 874, 941, 953 |
| BstNI | CCWGG | 3 cut(s) | 111, 1148, 1451 |
| BstPAI | GACNNNNGTC | 1 cut(s) | 392 |
| BstSCI | CCNGG | 4 cut(s) | 109, 1132, 1146, 1449 |
| BstSFI | CTRYAG | 1 cut(s) | 1308 |
| BstV1I | GCAGC | 7 cut(s) | 151, 340, 343, 877, 943, 1529, 1657 |
| BstV2I | GAAGAC | 2 cut(s) | 1357, 1416 |
| BstX2I | RGATCY | 2 cut(s) | 748, 842 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 512 |
| BstYI | RGATCY | 2 cut(s) | 748, 842 |
| Bsu15I | ATCGAT | 1 cut(s) | 1266 |
| BsuRI | GGCC | 4 cut(s) | 88, 108, 318, 1314 |
| BsuTUI | ATCGAT | 1 cut(s) | 1266 |
| BtsCI | GGATG | 5 cut(s) | 304, 540, 650, 1495, 1714 |
| BtsI | GCAGTG | 2 cut(s) | 654, 1735 |
| BtsIMutI | CAGTG | 6 cut(s) | 240, 397, 592, 654, 813, 1735 |
| BveI | ACCTGC | 2 cut(s) | 268, 1093 |
| Cac8I | GCNNGC | 5 cut(s) | 106, 288, 316, 1554, 1584 |
| CfoI | GCGC | 2 cut(s) | 944, 1670 |
| Cfr10I | RCCGGY | 1 cut(s) | 104 |
| Cfr13I | GGNCC | 3 cut(s) | 107, 994, 1739 |
| ClaI | ATCGAT | 1 cut(s) | 1266 |
| Csp6I | GTAC | 4 cut(s) | 257, 950, 1238, 1399 |
| CviAII | CATG | 7 cut(s) | 76, 124, 745, 821, 1471, 1681, 1699 |
| CviQI | GTAC | 4 cut(s) | 257, 950, 1238, 1399 |
| DdeI | CTNAG | 6 cut(s) | 279, 605, 660, 1242, 1296, 1587 |
| DinI | GGCGCC | 1 cut(s) | 943 |
| DpnI | GATC | 5 cut(s) | 423, 750, 844, 1145, 1247 |
| DpnII | GATC | 5 cut(s) | 421, 748, 842, 1143, 1245 |
| DrdI | GACNNNNNNGTC | 2 cut(s) | 1021, 1418 |
| DseDI | GACNNNNNNGTC | 2 cut(s) | 1021, 1418 |
| Eam1104I | CTCTTC | 3 cut(s) | 456, 729, 1590 |
| EarI | CTCTTC | 3 cut(s) | 456, 729, 1590 |
| Ecl136II | GAGCTC | 2 cut(s) | 602, 1621 |
| Eco130I | CCWWGG | 4 cut(s) | 1026, 1070, 1229, 1359 |
| Eco24I | GRGCYC | 2 cut(s) | 604, 1623 |
| Eco47I | GGWCC | 2 cut(s) | 994, 1739 |
| Eco53kI | GAGCTC | 2 cut(s) | 602, 1621 |
| Eco57I | CTGAAG | 1 cut(s) | 1614 |
| EcoICRI | GAGCTC | 2 cut(s) | 602, 1621 |
| EcoO109I | RGGNCCY | 1 cut(s) | 994 |
| EcoRII | CCWGG | 3 cut(s) | 109, 1146, 1449 |
| EcoT14I | CCWWGG | 4 cut(s) | 1026, 1070, 1229, 1359 |
| EcoT38I | GRGCYC | 2 cut(s) | 604, 1623 |
| EgeI | GGCGCC | 1 cut(s) | 943 |
| EheI | GGCGCC | 1 cut(s) | 943 |
| ErhI | CCWWGG | 4 cut(s) | 1026, 1070, 1229, 1359 |
| FaeI | CATG | 7 cut(s) | 79, 127, 748, 824, 1474, 1684, 1702 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 607, 639 |
| FaqI | GGGAC | 2 cut(s) | 241, 1725 |
| FatI | CATG | 7 cut(s) | 75, 123, 744, 820, 1470, 1680, 1698 |
| Fnu4HI | GCNGC | 8 cut(s) | 165, 354, 357, 866, 945, 957, 1518, 1671 |
| FokI | GGATG | 5 cut(s) | 311, 547, 637, 1502, 1721 |
| FriOI | GRGCYC | 2 cut(s) | 604, 1623 |
| Fsp4HI | GCNGC | 8 cut(s) | 165, 354, 357, 866, 945, 957, 1518, 1671 |
| FspBI | CTAG | 2 cut(s) | 849, 1568 |
| GlaI | GCGC | 2 cut(s) | 943, 1669 |
| GluI | GCNGC | 8 cut(s) | 165, 354, 357, 866, 945, 957, 1518, 1671 |
| GsaI | CCCAGC | 1 cut(s) | 306 |
| GsuI | CTGGAG | 3 cut(s) | 516, 1065, 1472 |
| HaeII | RGCGCY | 2 cut(s) | 945, 1671 |
| HaeIII | GGCC | 4 cut(s) | 88, 108, 318, 1314 |
| HapII | CCGG | 3 cut(s) | 105, 1134, 1212 |
| HhaI | GCGC | 2 cut(s) | 944, 1670 |
| Hin1I | GRCGYC | 1 cut(s) | 942 |
| Hin1II | CATG | 7 cut(s) | 79, 127, 748, 824, 1474, 1684, 1702 |
| Hin6I | GCGC | 2 cut(s) | 942, 1668 |
| HinP1I | GCGC | 2 cut(s) | 942, 1668 |
| HindIII | AAGCTT | 1 cut(s) | 1508 |
| HinfI | GANTC | 8 cut(s) | 56, 275, 541, 643, 680, 853, 1015, 1436 |
| HpaII | CCGG | 3 cut(s) | 105, 1134, 1212 |
| HphI | GGTGA | 7 cut(s) | 125, 493, 527, 550, 709, 983, 1172 |
| Hpy188I | TCNGA | 5 cut(s) | 282, 663, 1123, 1245, 1408 |
| HpyAV | CCTTC | 8 cut(s) | 376, 461, 478, 979, 1090, 1423, 1609, 1619 |
| HpyCH4III | ACNGT | 6 cut(s) | 235, 256, 817, 1060, 1252, 1356 |
| HpyCH4IV | ACGT | 1 cut(s) | 1287 |
| HpyF10VI | GCNNNNNNNGC | 4 cut(s) | 362, 874, 941, 953 |
| HpyF3I | CTNAG | 6 cut(s) | 279, 605, 660, 1242, 1296, 1587 |
| HpySE526I | ACGT | 1 cut(s) | 1287 |
| Hsp92I | GRCGYC | 1 cut(s) | 942 |
| Hsp92II | CATG | 7 cut(s) | 79, 127, 748, 824, 1474, 1684, 1702 |
| HspAI | GCGC | 2 cut(s) | 942, 1668 |
| KasI | GGCGCC | 1 cut(s) | 941 |
| Kpn2I | TCCGGA | 1 cut(s) | 1211 |
| KroI | GCCGGC | 1 cut(s) | 104 |
| KroNI | GCCGGC | 1 cut(s) | 106 |
| Kzo9I | GATC | 5 cut(s) | 421, 748, 842, 1143, 1245 |
| LguI | GCTCTTC | 1 cut(s) | 729 |
| LmnI | GCTCC | 5 cut(s) | 333, 769, 1099, 1618, 1678 |
| Lsp1109I | GCAGC | 7 cut(s) | 151, 340, 343, 877, 943, 1529, 1657 |
| LweI | GCATC | 7 cut(s) | 13, 189, 289, 784, 1258, 1504, 1531 |
| MaeI | CTAG | 2 cut(s) | 849, 1568 |
| MaeII | ACGT | 1 cut(s) | 1287 |
| MaeIII | GTNAC | 5 cut(s) | 53, 113, 133, 250, 1012 |
| MalI | GATC | 5 cut(s) | 423, 750, 844, 1145, 1247 |
| MboI | GATC | 5 cut(s) | 421, 748, 842, 1143, 1245 |
| MfeI | CAATTG | 3 cut(s) | 459, 869, 1545 |
| MflI | RGATCY | 2 cut(s) | 748, 842 |
| MhlI | GDGCHC | 5 cut(s) | 330, 604, 815, 1065, 1623 |
| Mly113I | GGCGCC | 1 cut(s) | 942 |
| MlyI | GAGTC | 6 cut(s) | 50, 269, 637, 674, 1009, 1445 |
| MmeI | TCCRAC | 3 cut(s) | 1112, 1386, 1742 |
| MroI | TCCGGA | 1 cut(s) | 1211 |
| MroNI | GCCGGC | 1 cut(s) | 104 |
| MseI | TTAA | 5 cut(s) | 35, 614, 915, 1485, 1762 |
| MslI | CAYNNNNRTG | 2 cut(s) | 510, 1685 |
| MspA1I | CMGCKG | 1 cut(s) | 947 |
| MspI | CCGG | 3 cut(s) | 105, 1134, 1212 |
| MspR9I | CCNGG | 4 cut(s) | 111, 1134, 1148, 1451 |
| MunI | CAATTG | 3 cut(s) | 459, 869, 1545 |
| Mva1269I | GAATGC | 2 cut(s) | 247, 1278 |
| MvaI | CCWGG | 3 cut(s) | 111, 1148, 1451 |
| MwoI | GCNNNNNNNGC | 4 cut(s) | 362, 874, 941, 953 |
| NaeI | GCCGGC | 1 cut(s) | 106 |
| NarI | GGCGCC | 1 cut(s) | 942 |
| NciI | CCSGG | 1 cut(s) | 1134 |
| NdeII | GATC | 5 cut(s) | 421, 748, 842, 1143, 1245 |
| NgoMIV | GCCGGC | 1 cut(s) | 104 |
| NlaIII | CATG | 7 cut(s) | 79, 127, 748, 824, 1474, 1684, 1702 |
| NlaIV | GGNNCC | 4 cut(s) | 436, 771, 943, 1741 |
| NmuCI | GTSAC | 3 cut(s) | 53, 113, 1012 |
| PciSI | GCTCTTC | 1 cut(s) | 729 |
| PctI | GAATGC | 2 cut(s) | 247, 1278 |
| PdiI | GCCGGC | 1 cut(s) | 106 |
| PfeI | GAWTC | 2 cut(s) | 541, 853 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 1724 |
| PkrI | GCNGC | 8 cut(s) | 166, 355, 358, 867, 946, 958, 1519, 1672 |
| PleI | GAGTC | 6 cut(s) | 50, 269, 637, 674, 1009, 1444 |
| PluTI | GGCGCC | 1 cut(s) | 945 |
| PpsI | GAGTC | 6 cut(s) | 50, 269, 637, 674, 1009, 1444 |
| PpuMI | RGGWCCY | 1 cut(s) | 994 |
| PshAI | GACNNNNGTC | 1 cut(s) | 392 |
| PsiI | TTATAA | 2 cut(s) | 14, 1467 |
| Psp124BI | GAGCTC | 2 cut(s) | 604, 1623 |
| Psp5II | RGGWCCY | 1 cut(s) | 994 |
| Psp6I | CCWGG | 3 cut(s) | 109, 1146, 1449 |
| PspFI | CCCAGC | 1 cut(s) | 302 |
| PspGI | CCWGG | 3 cut(s) | 109, 1146, 1449 |
| PspN4I | GGNNCC | 4 cut(s) | 436, 771, 943, 1741 |
| PspPI | GGNCC | 3 cut(s) | 107, 994, 1739 |
| PspPPI | RGGWCCY | 1 cut(s) | 994 |
| PsuI | RGATCY | 2 cut(s) | 748, 842 |
| RsaI | GTAC | 4 cut(s) | 258, 951, 1239, 1400 |
| RsaNI | GTAC | 4 cut(s) | 257, 950, 1238, 1399 |
| RseI | CAYNNNNRTG | 2 cut(s) | 510, 1685 |
| SacI | GAGCTC | 2 cut(s) | 604, 1623 |
| SapI | GCTCTTC | 1 cut(s) | 729 |
| SaqAI | TTAA | 5 cut(s) | 35, 614, 915, 1485, 1762 |
| SatI | GCNGC | 8 cut(s) | 165, 354, 357, 866, 945, 957, 1518, 1671 |
| Sau3AI | GATC | 5 cut(s) | 421, 748, 842, 1143, 1245 |
| Sau96I | GGNCC | 3 cut(s) | 107, 994, 1739 |
| SchI | GAGTC | 6 cut(s) | 50, 269, 637, 674, 1009, 1445 |
| ScrFI | CCNGG | 4 cut(s) | 111, 1134, 1148, 1451 |
| SduI | GDGCHC | 5 cut(s) | 330, 604, 815, 1065, 1623 |
| SfaNI | GCATC | 7 cut(s) | 13, 189, 289, 784, 1258, 1504, 1531 |
| SfcI | CTRYAG | 1 cut(s) | 1308 |
| SfoI | GGCGCC | 1 cut(s) | 943 |
| SinI | GGWCC | 2 cut(s) | 994, 1739 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 510, 1685 |
| SsiI | CCGC | 4 cut(s) | 31, 167, 945, 1391 |
| SspDI | GGCGCC | 1 cut(s) | 941 |
| SspMI | CTAG | 2 cut(s) | 849, 1568 |
| SstI | GAGCTC | 2 cut(s) | 604, 1623 |
| StyD4I | CCNGG | 4 cut(s) | 109, 1132, 1146, 1449 |
| StyI | CCWWGG | 4 cut(s) | 1026, 1070, 1229, 1359 |
| TaaI | ACNGT | 6 cut(s) | 235, 256, 817, 1060, 1252, 1356 |
| TaiI | ACGT | 1 cut(s) | 1290 |
| TaqI | TCGA | 4 cut(s) | 672, 699, 1266, 1623 |
| TatI | WGTACW | 2 cut(s) | 949, 1398 |
| TauI | GCSGC | 1 cut(s) | 947 |
| TfiI | GAWTC | 2 cut(s) | 541, 853 |
| Tru1I | TTAA | 5 cut(s) | 35, 614, 915, 1485, 1762 |
| Tru9I | TTAA | 5 cut(s) | 35, 614, 915, 1485, 1762 |
| TscAI | CASTG | 6 cut(s) | 240, 397, 592, 661, 820, 1735 |
| TseFI | GTSAC | 3 cut(s) | 53, 113, 1012 |
| TseI | GCWGC | 7 cut(s) | 164, 353, 356, 865, 956, 1517, 1670 |
| Tsp45I | GTSAC | 3 cut(s) | 53, 113, 1012 |
| TspDTI | ATGAA | 2 cut(s) | 92, 939 |
| TspGWI | ACGGA | 2 cut(s) | 320, 906 |
| TspRI | CASTG | 6 cut(s) | 240, 397, 592, 661, 820, 1735 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 1724 |
| VpaK11BI | GGWCC | 2 cut(s) | 994, 1739 |
| XapI | RAATTY | 1 cut(s) | 1444 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 1447 |
| XspI | CTAG | 2 cut(s) | 849, 1568 |