Heat shock cognate 70 kDa
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AasI | GACNNNNNNGTC | 1 cut(s) | 121 |
| Acc65I | GGTACC | 1 cut(s) | 887 |
| AccB1I | GGYRCC | 1 cut(s) | 887 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 331 |
| AccI | GTMKAC | 1 cut(s) | 408 |
| AclWI | GGATC | 4 cut(s) | 38, 780, 1080, 1255 |
| AcoI | YGGCCR | 2 cut(s) | 183, 275 |
| AcsI | RAATTY | 5 cut(s) | 749, 920, 1044, 1278, 1559 |
| AcuI | CTGAAG | 2 cut(s) | 378, 423 |
| AdeI | CACNNNGTG | 1 cut(s) | 473 |
| AfaI | GTAC | 4 cut(s) | 50, 889, 1316, 1525 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 331, 1161 |
| AjnI | CCWGG | 2 cut(s) | 36, 177 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 396, 428 |
| AleI | CACNNNNGTG | 1 cut(s) | 78 |
| AluBI | AGCT | 9 cut(s) | 153, 170, 271, 479, 1063, 1148, 1391, 1421, 1597 |
| AluI | AGCT | 9 cut(s) | 153, 170, 271, 479, 1063, 1148, 1391, 1421, 1597 |
| Alw26I | GTCTC | 6 cut(s) | 389, 642, 684, 705, 1223, 1649 |
| AlwI | GGATC | 4 cut(s) | 38, 780, 1080, 1255 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 1075 |
| AoxI | GGCC | 2 cut(s) | 183, 275 |
| ApoI | RAATTY | 5 cut(s) | 749, 920, 1044, 1278, 1559 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 1499 |
| Asp718I | GGTACC | 1 cut(s) | 887 |
| AspS9I | GGNCC | 1 cut(s) | 322 |
| AsuHPI | GGTGA | 5 cut(s) | 130, 689, 1198, 1520, 1696 |
| AsuII | TTCGAA | 1 cut(s) | 924 |
| AvaII | GGWCC | 1 cut(s) | 322 |
| BalI | TGGCCA | 2 cut(s) | 185, 277 |
| BanI | GGYRCC | 1 cut(s) | 887 |
| BauI | CACGAG | 1 cut(s) | 52 |
| BbsI | GAAGAC | 1 cut(s) | 366 |
| BccI | CCATC | 4 cut(s) | 158, 367, 557, 988 |
| BceAI | ACGGC | 1 cut(s) | 1486 |
| BcgI | CGANNNNNNTGC | 4 cut(s) | 59, 93, 521, 555 |
| BciT130I | CCWGG | 2 cut(s) | 38, 179 |
| BclI | TGATCA | 2 cut(s) | 87, 525 |
| BcoDI | GTCTC | 6 cut(s) | 389, 642, 684, 705, 1223, 1649 |
| BfaI | CTAG | 2 cut(s) | 1041, 1275 |
| BfmI | CTRYAG | 2 cut(s) | 543, 1149 |
| BlpI | GCTNAGC | 2 cut(s) | 644, 1598 |
| Bme1390I | CCNGG | 2 cut(s) | 38, 179 |
| Bme18I | GGWCC | 1 cut(s) | 322 |
| BmgT120I | GGNCC | 1 cut(s) | 322 |
| BmiI | GGNNCC | 2 cut(s) | 43, 889 |
| BmrFI | CCNGG | 2 cut(s) | 38, 179 |
| BmsI | GCATC | 6 cut(s) | 202, 378, 889, 976, 1167, 1453 |
| BpiI | GAAGAC | 1 cut(s) | 366 |
| BpmI | CTGGAG | 1 cut(s) | 1401 |
| Bpu1102I | GCTNAGC | 2 cut(s) | 644, 1598 |
| Bpu14I | TTCGAA | 1 cut(s) | 924 |
| BpuEI | CTTGAG | 2 cut(s) | 1177, 1218 |
| Bsa29I | ATCGAT | 1 cut(s) | 371 |
| BsaAI | YACGTR | 1 cut(s) | 48 |
| BsaBI | GATNNNNATC | 1 cut(s) | 998 |
| BsaJI | CCNNGG | 5 cut(s) | 37, 700, 789, 1050, 1425 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 331, 1161 |
| Bse1I | ACTGG | 1 cut(s) | 1510 |
| Bse8I | GATNNNNATC | 1 cut(s) | 998 |
| BseBI | CCWGG | 2 cut(s) | 38, 179 |
| BseCI | ATCGAT | 1 cut(s) | 371 |
| BseDI | CCNNGG | 5 cut(s) | 37, 700, 789, 1050, 1425 |
| BseGI | GGATG | 3 cut(s) | 991, 1182, 1207 |
| BseJI | GATNNNNATC | 1 cut(s) | 998 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 331, 1161 |
| BseMII | CTCAG | 1 cut(s) | 1482 |
| BseNI | ACTGG | 1 cut(s) | 1510 |
| BseRI | GAGGAG | 1 cut(s) | 847 |
| BsgI | GTGCAG | 1 cut(s) | 1076 |
| Bsh1285I | CGRYCG | 1 cut(s) | 79 |
| BshFI | GGCC | 2 cut(s) | 185, 277 |
| BshNI | GGYRCC | 1 cut(s) | 887 |
| BshVI | ATCGAT | 1 cut(s) | 371 |
| BsiEI | CGRYCG | 1 cut(s) | 79 |
| BsiSI | CCGG | 1 cut(s) | 504 |
| BslFI | GGGAC | 2 cut(s) | 431, 1347 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 331, 1161 |
| BsmAI | GTCTC | 6 cut(s) | 389, 642, 684, 705, 1223, 1649 |
| BsmFI | GGGAC | 2 cut(s) | 431, 1347 |
| BsmI | GAATGC | 2 cut(s) | 436, 1473 |
| BsnI | GGCC | 2 cut(s) | 185, 277 |
| Bsp119I | TTCGAA | 1 cut(s) | 924 |
| Bsp1407I | TGTACA | 1 cut(s) | 1523 |
| Bsp143I | GATC | 7 cut(s) | 30, 87, 525, 772, 1072, 1260, 1635 |
| Bsp1720I | GCTNAGC | 2 cut(s) | 644, 1598 |
| BspANI | GGCC | 2 cut(s) | 185, 277 |
| BspCNI | CTCAG | 1 cut(s) | 1483 |
| BspDI | ATCGAT | 1 cut(s) | 371 |
| BspLI | GGNNCC | 2 cut(s) | 43, 889 |
| BspMAI | CTGCAG | 2 cut(s) | 547, 1153 |
| BspPI | GGATC | 4 cut(s) | 38, 780, 1080, 1255 |
| BspT104I | TTCGAA | 1 cut(s) | 924 |
| BspT107I | GGYRCC | 1 cut(s) | 887 |
| BsrGI | TGTACA | 1 cut(s) | 1523 |
| BsrI | ACTGG | 1 cut(s) | 1510 |
| BssECI | CCNNGG | 5 cut(s) | 37, 700, 789, 1050, 1425 |
| BssMI | GATC | 7 cut(s) | 30, 87, 525, 772, 1072, 1260, 1635 |
| BssSI | CACGAG | 1 cut(s) | 52 |
| BssT1I | CCWWGG | 4 cut(s) | 700, 789, 1050, 1425 |
| Bst2BI | CACGAG | 1 cut(s) | 52 |
| Bst2UI | CCWGG | 2 cut(s) | 38, 179 |
| Bst4CI | ACNGT | 7 cut(s) | 77, 205, 348, 424, 445, 685, 853 |
| Bst6I | CTCTTC | 1 cut(s) | 212 |
| BstAPI | GCANNNNNTGC | 2 cut(s) | 1133, 1479 |
| BstAUI | TGTACA | 1 cut(s) | 1523 |
| BstBAI | YACGTR | 1 cut(s) | 48 |
| BstBI | TTCGAA | 1 cut(s) | 924 |
| BstC8I | GCNNGC | 1 cut(s) | 817 |
| BstDEI | CTNAG | 7 cut(s) | 644, 776, 797, 990, 1387, 1491, 1598 |
| BstF5I | GGATG | 3 cut(s) | 991, 1182, 1207 |
| BstKTI | GATC | 7 cut(s) | 33, 90, 528, 775, 1075, 1263, 1638 |
| BstMAI | GTCTC | 6 cut(s) | 389, 642, 684, 705, 1223, 1649 |
| BstMBI | GATC | 7 cut(s) | 30, 87, 525, 772, 1072, 1260, 1635 |
| BstMCI | CGRYCG | 1 cut(s) | 79 |
| BstMWI | GCNNNNNNNGC | 5 cut(s) | 159, 551, 1133, 1145, 1479 |
| BstNI | CCWGG | 2 cut(s) | 38, 179 |
| BstNSI | RCATGY | 4 cut(s) | 23, 491, 815, 1694 |
| BstSCI | CCNGG | 2 cut(s) | 36, 177 |
| BstSFI | CTRYAG | 2 cut(s) | 543, 1149 |
| BstV2I | GAAGAC | 1 cut(s) | 366 |
| BstX2I | RGATCY | 2 cut(s) | 772, 1072 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 319 |
| BstYI | RGATCY | 2 cut(s) | 772, 1072 |
| Bsu15I | ATCGAT | 1 cut(s) | 371 |
| BsuRI | GGCC | 2 cut(s) | 185, 277 |
| BsuTUI | ATCGAT | 1 cut(s) | 371 |
| BtgZI | GCGATG | 2 cut(s) | 177, 535 |
| BtsCI | GGATG | 3 cut(s) | 991, 1182, 1207 |
| BtsI | GCAGTG | 3 cut(s) | 69, 498, 1128 |
| BtsIMutI | CAGTG | 5 cut(s) | 69, 498, 849, 1128, 1503 |
| Cac8I | GCNNGC | 1 cut(s) | 817 |
| CaiI | CAGNNNCTG | 1 cut(s) | 1075 |
| Cfr13I | GGNCC | 1 cut(s) | 322 |
| ClaI | ATCGAT | 1 cut(s) | 371 |
| CseI | GACGC | 1 cut(s) | 997 |
| CsiI | ACCWGGT | 1 cut(s) | 177 |
| Csp6I | GTAC | 4 cut(s) | 49, 888, 1315, 1524 |
| CviAII | CATG | 8 cut(s) | 20, 313, 331, 488, 812, 1013, 1249, 1691 |
| CviQI | GTAC | 4 cut(s) | 49, 888, 1315, 1524 |
| DdeI | CTNAG | 7 cut(s) | 644, 776, 797, 990, 1387, 1491, 1598 |
| DpnI | GATC | 7 cut(s) | 32, 89, 527, 774, 1074, 1262, 1637 |
| DpnII | GATC | 7 cut(s) | 30, 87, 525, 772, 1072, 1260, 1635 |
| DraIII | CACNNNGTG | 1 cut(s) | 473 |
| DrdI | GACNNNNNNGTC | 1 cut(s) | 121 |
| DseDI | GACNNNNNNGTC | 1 cut(s) | 121 |
| EaeI | YGGCCR | 2 cut(s) | 183, 275 |
| Eam1104I | CTCTTC | 1 cut(s) | 212 |
| EarI | CTCTTC | 1 cut(s) | 212 |
| Eco130I | CCWWGG | 4 cut(s) | 700, 789, 1050, 1425 |
| Eco32I | GATATC | 1 cut(s) | 1000 |
| Eco47I | GGWCC | 1 cut(s) | 322 |
| Eco57I | CTGAAG | 2 cut(s) | 378, 423 |
| EcoRI | GAATTC | 4 cut(s) | 749, 1044, 1278, 1559 |
| EcoRII | CCWGG | 2 cut(s) | 36, 177 |
| EcoRV | GATATC | 1 cut(s) | 1000 |
| EcoT14I | CCWWGG | 4 cut(s) | 700, 789, 1050, 1425 |
| ErhI | CCWWGG | 4 cut(s) | 700, 789, 1050, 1425 |
| FaeI | CATG | 8 cut(s) | 23, 316, 334, 491, 815, 1016, 1252, 1694 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 929, 961 |
| FaqI | GGGAC | 2 cut(s) | 431, 1347 |
| FatI | CATG | 8 cut(s) | 19, 312, 330, 487, 811, 1012, 1248, 1690 |
| FauNDI | CATATG | 3 cut(s) | 522, 844, 1129 |
| FbaI | TGATCA | 2 cut(s) | 87, 525 |
| FblI | GTMKAC | 1 cut(s) | 408 |
| FokI | GGATG | 3 cut(s) | 998, 1189, 1214 |
| FspBI | CTAG | 2 cut(s) | 1041, 1275 |
| GsuI | CTGGAG | 1 cut(s) | 1401 |
| HaeIII | GGCC | 2 cut(s) | 185, 277 |
| HapII | CCGG | 1 cut(s) | 504 |
| HgaI | GACGC | 1 cut(s) | 997 |
| Hin1II | CATG | 8 cut(s) | 23, 316, 334, 491, 815, 1016, 1252, 1694 |
| HincII | GTYRAC | 1 cut(s) | 733 |
| HindII | GTYRAC | 1 cut(s) | 733 |
| HindIII | AAGCTT | 1 cut(s) | 269 |
| HinfI | GANTC | 6 cut(s) | 118, 143, 507, 517, 1231, 1360 |
| HpaII | CCGG | 1 cut(s) | 504 |
| HphI | GGTGA | 5 cut(s) | 130, 689, 1198, 1520, 1696 |
| Hpy166II | GTNNAC | 5 cut(s) | 325, 409, 733, 1503, 1524 |
| Hpy188I | TCNGA | 1 cut(s) | 148 |
| Hpy188III | TCNNGA | 5 cut(s) | 338, 754, 1041, 1112, 1564 |
| Hpy8I | GTNNAC | 5 cut(s) | 325, 409, 733, 1503, 1524 |
| HpyAV | CCTTC | 9 cut(s) | 145, 287, 565, 670, 817, 976, 1584, 1670, 1674 |
| HpyCH4III | ACNGT | 7 cut(s) | 77, 205, 348, 424, 445, 685, 853 |
| HpyCH4IV | ACGT | 4 cut(s) | 47, 129, 768, 1245 |
| HpyF10VI | GCNNNNNNNGC | 5 cut(s) | 159, 551, 1133, 1145, 1479 |
| HpyF3I | CTNAG | 7 cut(s) | 644, 776, 797, 990, 1387, 1491, 1598 |
| HpySE526I | ACGT | 4 cut(s) | 47, 129, 768, 1245 |
| Hsp92II | CATG | 8 cut(s) | 23, 316, 334, 491, 815, 1016, 1252, 1694 |
| KpnI | GGTACC | 1 cut(s) | 891 |
| Ksp22I | TGATCA | 2 cut(s) | 87, 525 |
| Kzo9I | GATC | 7 cut(s) | 30, 87, 525, 772, 1072, 1260, 1635 |
| LmnI | GCTCC | 3 cut(s) | 167, 1426, 1594 |
| LweI | GCATC | 6 cut(s) | 202, 378, 889, 976, 1167, 1453 |
| MabI | ACCWGGT | 1 cut(s) | 177 |
| MaeI | CTAG | 2 cut(s) | 1041, 1275 |
| MaeII | ACGT | 4 cut(s) | 47, 129, 768, 1245 |
| MaeIII | GTNAC | 6 cut(s) | 242, 439, 853, 1204, 1406, 1624 |
| MalI | GATC | 7 cut(s) | 32, 89, 527, 774, 1074, 1262, 1637 |
| MboI | GATC | 7 cut(s) | 30, 87, 525, 772, 1072, 1260, 1635 |
| MboII | GAAGA | 9 cut(s) | 26, 229, 246, 371, 439, 937, 938, 1568, 1628 |
| MflI | RGATCY | 2 cut(s) | 772, 1072 |
| MlsI | TGGCCA | 2 cut(s) | 185, 277 |
| MluCI | AATT | 7 cut(s) | 749, 867, 920, 1044, 1278, 1381, 1559 |
| MluNI | TGGCCA | 2 cut(s) | 185, 277 |
| MlyI | GAGTC | 4 cut(s) | 112, 501, 1225, 1369 |
| MmeI | TCCRAC | 2 cut(s) | 222, 667 |
| Mox20I | TGGCCA | 2 cut(s) | 185, 277 |
| MroXI | GAANNNNTTC | 1 cut(s) | 1499 |
| MscI | TGGCCA | 2 cut(s) | 185, 277 |
| MseI | TTAA | 4 cut(s) | 174, 806, 972, 1107 |
| MslI | CAYNNNNRTG | 3 cut(s) | 78, 317, 1289 |
| Msp20I | TGGCCA | 2 cut(s) | 185, 277 |
| MspI | CCGG | 1 cut(s) | 504 |
| MspR9I | CCNGG | 2 cut(s) | 38, 179 |
| Mva1269I | GAATGC | 2 cut(s) | 436, 1473 |
| MvaI | CCWGG | 2 cut(s) | 38, 179 |
| MwoI | GCNNNNNNNGC | 5 cut(s) | 159, 551, 1133, 1145, 1479 |
| NdeI | CATATG | 3 cut(s) | 522, 844, 1129 |
| NdeII | GATC | 7 cut(s) | 30, 87, 525, 772, 1072, 1260, 1635 |
| NlaIII | CATG | 8 cut(s) | 23, 316, 334, 491, 815, 1016, 1252, 1694 |
| NlaIV | GGNNCC | 2 cut(s) | 43, 889 |
| NmuCI | GTSAC | 4 cut(s) | 242, 853, 1204, 1624 |
| NspI | RCATGY | 4 cut(s) | 23, 491, 815, 1694 |
| NspV | TTCGAA | 1 cut(s) | 924 |
| OliI | CACNNNNGTG | 1 cut(s) | 78 |
| PctI | GAATGC | 2 cut(s) | 436, 1473 |
| PdmI | GAANNNNTTC | 1 cut(s) | 1499 |
| PfeI | GAWTC | 2 cut(s) | 143, 517 |
| PflFI | GACNNNGTC | 2 cut(s) | 121, 1397 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 331 |
| PleI | GAGTC | 4 cut(s) | 112, 501, 1225, 1368 |
| PpsI | GAGTC | 4 cut(s) | 112, 501, 1225, 1368 |
| Ppu21I | YACGTR | 1 cut(s) | 48 |
| Psp6I | CCWGG | 2 cut(s) | 36, 177 |
| PspGI | CCWGG | 2 cut(s) | 36, 177 |
| PspN4I | GGNNCC | 2 cut(s) | 43, 889 |
| PspPI | GGNCC | 1 cut(s) | 322 |
| PstI | CTGCAG | 2 cut(s) | 547, 1153 |
| PstNI | CAGNNNCTG | 1 cut(s) | 1075 |
| PsuI | RGATCY | 2 cut(s) | 772, 1072 |
| PsyI | GACNNNGTC | 2 cut(s) | 121, 1397 |
| RsaI | GTAC | 4 cut(s) | 50, 889, 1316, 1525 |
| RsaNI | GTAC | 4 cut(s) | 49, 888, 1315, 1524 |
| RseI | CAYNNNNRTG | 3 cut(s) | 78, 317, 1289 |
| SaqAI | TTAA | 4 cut(s) | 174, 806, 972, 1107 |
| Sau3AI | GATC | 7 cut(s) | 30, 87, 525, 772, 1072, 1260, 1635 |
| Sau96I | GGNCC | 1 cut(s) | 322 |
| SchI | GAGTC | 4 cut(s) | 112, 501, 1225, 1369 |
| ScrFI | CCNGG | 2 cut(s) | 38, 179 |
| SexAI | ACCWGGT | 1 cut(s) | 177 |
| SfaNI | GCATC | 6 cut(s) | 202, 378, 889, 976, 1167, 1453 |
| SfcI | CTRYAG | 2 cut(s) | 543, 1149 |
| SfuI | TTCGAA | 1 cut(s) | 924 |
| SinI | GGWCC | 1 cut(s) | 322 |
| SmiMI | CAYNNNNRTG | 3 cut(s) | 78, 317, 1289 |
| SmlI | CTYRAG | 2 cut(s) | 1156, 1233 |
| SmoI | CTYRAG | 2 cut(s) | 1156, 1233 |
| Sse9I | AATT | 7 cut(s) | 749, 867, 920, 1044, 1278, 1381, 1559 |
| SspMI | CTAG | 2 cut(s) | 1041, 1275 |
| StyD4I | CCNGG | 2 cut(s) | 36, 177 |
| StyI | CCWWGG | 4 cut(s) | 700, 789, 1050, 1425 |
| TaaI | ACNGT | 7 cut(s) | 77, 205, 348, 424, 445, 685, 853 |
| TaiI | ACGT | 4 cut(s) | 50, 132, 771, 1248 |
| TaqI | TCGA | 3 cut(s) | 33, 371, 924 |
| TasI | AATT | 7 cut(s) | 749, 867, 920, 1044, 1278, 1381, 1559 |
| TatI | WGTACW | 1 cut(s) | 1523 |
| TfiI | GAWTC | 2 cut(s) | 143, 517 |
| Tru1I | TTAA | 4 cut(s) | 174, 806, 972, 1107 |
| Tru9I | TTAA | 4 cut(s) | 174, 806, 972, 1107 |
| TscAI | CASTG | 5 cut(s) | 69, 505, 856, 1128, 1510 |
| TseFI | GTSAC | 4 cut(s) | 242, 853, 1204, 1624 |
| Tsp45I | GTSAC | 4 cut(s) | 242, 853, 1204, 1624 |
| TspDTI | ATGAA | 8 cut(s) | 281, 357, 509, 831, 937, 1026, 1182, 1307 |
| TspGWI | ACGGA | 1 cut(s) | 111 |
| TspRI | CASTG | 5 cut(s) | 69, 505, 856, 1128, 1510 |
| Tth111I | GACNNNGTC | 2 cut(s) | 121, 1397 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 331 |
| VpaK11BI | GGWCC | 1 cut(s) | 322 |
| XapI | RAATTY | 5 cut(s) | 749, 920, 1044, 1278, 1559 |
| XbaI | TCTAGA | 1 cut(s) | 1040 |
| XceI | RCATGY | 4 cut(s) | 23, 491, 815, 1694 |
| XmiI | GTMKAC | 1 cut(s) | 408 |
| XmnI | GAANNNNTTC | 1 cut(s) | 1499 |
| XspI | CTAG | 2 cut(s) | 1041, 1275 |