Heat shock cognate 70 kDa
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 155 |
| Acc36I | ACCTGC | 1 cut(s) | 449 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 712 |
| AciI | CCGC | 2 cut(s) | 148, 171 |
| AcsI | RAATTY | 2 cut(s) | 545, 669 |
| AfaI | GTAC | 3 cut(s) | 253, 636, 681 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 712 |
| AgsI | TTSAA | 5 cut(s) | 88, 491, 551, 574, 770 |
| AhlI | ACTAGT | 1 cut(s) | 527 |
| AjnI | CCWGG | 1 cut(s) | 705 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 377, 409 |
| AluBI | AGCT | 4 cut(s) | 29, 419, 589, 773 |
| AluI | AGCT | 4 cut(s) | 29, 419, 589, 773 |
| Alw21I | GWGCWC | 1 cut(s) | 421 |
| Alw26I | GTCTC | 3 cut(s) | 309, 454, 473 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 482 |
| Ama87I | CYCGRG | 1 cut(s) | 537 |
| AoxI | GGCC | 5 cut(s) | 33, 133, 187, 201, 303 |
| ApeKI | GCWGC | 3 cut(s) | 173, 761, 773 |
| ApoI | RAATTY | 2 cut(s) | 545, 669 |
| AseI | ATTAAT | 1 cut(s) | 732 |
| AspS9I | GGNCC | 1 cut(s) | 397 |
| AsuHPI | GGTGA | 3 cut(s) | 314, 344, 367 |
| AvaI | CYCGRG | 1 cut(s) | 537 |
| AvaII | GGWCC | 1 cut(s) | 397 |
| BanII | GRGCYC | 1 cut(s) | 421 |
| BbsI | GAAGAC | 1 cut(s) | 342 |
| Bbv12I | GWGCWC | 1 cut(s) | 421 |
| BbvCI | CCTCAGC | 1 cut(s) | 30 |
| BbvI | GCAGC | 3 cut(s) | 185, 748, 760 |
| BccI | CCATC | 1 cut(s) | 706 |
| BciT130I | CCWGG | 1 cut(s) | 707 |
| BcoDI | GTCTC | 3 cut(s) | 309, 454, 473 |
| BcuI | ACTAGT | 1 cut(s) | 527 |
| BfaI | CTAG | 3 cut(s) | 414, 528, 666 |
| BfuAI | ACCTGC | 1 cut(s) | 449 |
| BisI | GCNGC | 4 cut(s) | 171, 174, 762, 774 |
| BlsI | GCNGC | 4 cut(s) | 172, 175, 763, 775 |
| Bme1390I | CCNGG | 1 cut(s) | 707 |
| Bme18I | GGWCC | 1 cut(s) | 397 |
| BmeT110I | CYCGRG | 1 cut(s) | 537 |
| BmgT120I | GGNCC | 1 cut(s) | 397 |
| BmrFI | CCNGG | 1 cut(s) | 707 |
| BmsI | GCATC | 5 cut(s) | 6, 106, 135, 514, 601 |
| BpiI | GAAGAC | 1 cut(s) | 342 |
| BpmI | CTGGAG | 2 cut(s) | 333, 626 |
| Bpu10I | CCTNAGC | 1 cut(s) | 30 |
| BsaJI | CCNNGG | 3 cut(s) | 40, 325, 706 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 712 |
| Bse1I | ACTGG | 2 cut(s) | 204, 286 |
| Bse3DI | GCAATG | 2 cut(s) | 126, 177 |
| BseBI | CCWGG | 1 cut(s) | 707 |
| BseDI | CCNNGG | 3 cut(s) | 40, 325, 706 |
| BseGI | GGATG | 2 cut(s) | 616, 717 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 712 |
| BseMI | GCAATG | 2 cut(s) | 126, 177 |
| BseMII | CTCAG | 1 cut(s) | 21 |
| BseNI | ACTGG | 2 cut(s) | 204, 286 |
| BseRI | GAGGAG | 1 cut(s) | 472 |
| BseXI | GCAGC | 3 cut(s) | 185, 748, 760 |
| BshFI | GGCC | 5 cut(s) | 35, 135, 189, 203, 305 |
| BsiHKAI | GWGCWC | 1 cut(s) | 421 |
| BsiHKCI | CYCGRG | 1 cut(s) | 537 |
| BslFI | GGGAC | 1 cut(s) | 59 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 712 |
| BsmAI | GTCTC | 3 cut(s) | 309, 454, 473 |
| BsmFI | GGGAC | 1 cut(s) | 59 |
| BsnI | GGCC | 5 cut(s) | 35, 135, 189, 203, 305 |
| BsoBI | CYCGRG | 1 cut(s) | 537 |
| Bsp1286I | GDGCHC | 1 cut(s) | 421 |
| Bsp1407I | TGTACA | 1 cut(s) | 634 |
| BspACI | CCGC | 2 cut(s) | 148, 171 |
| BspANI | GGCC | 5 cut(s) | 35, 135, 189, 203, 305 |
| BspCNI | CTCAG | 1 cut(s) | 22 |
| BspMI | ACCTGC | 1 cut(s) | 449 |
| BspQI | GCTCTTC | 1 cut(s) | 667 |
| BsrDI | GCAATG | 2 cut(s) | 126, 177 |
| BsrGI | TGTACA | 1 cut(s) | 634 |
| BsrI | ACTGG | 2 cut(s) | 204, 286 |
| BssECI | CCNNGG | 3 cut(s) | 40, 325, 706 |
| BssT1I | CCWWGG | 2 cut(s) | 40, 325 |
| Bst2UI | CCWGG | 1 cut(s) | 707 |
| Bst4CI | ACNGT | 4 cut(s) | 52, 73, 365, 500 |
| Bst6I | CTCTTC | 1 cut(s) | 667 |
| BstAPI | GCANNNNNTGC | 2 cut(s) | 179, 758 |
| BstAUI | TGTACA | 1 cut(s) | 634 |
| BstC8I | GCNNGC | 1 cut(s) | 133 |
| BstDEI | CTNAG | 3 cut(s) | 30, 401, 422 |
| BstF5I | GGATG | 2 cut(s) | 616, 717 |
| BstMAI | GTCTC | 3 cut(s) | 309, 454, 473 |
| BstMWI | GCNNNNNNNGC | 4 cut(s) | 132, 179, 758, 770 |
| BstNI | CCWGG | 1 cut(s) | 707 |
| BstNSI | RCATGY | 1 cut(s) | 756 |
| BstSCI | CCNGG | 1 cut(s) | 705 |
| BstV1I | GCAGC | 3 cut(s) | 185, 748, 760 |
| BstV2I | GAAGAC | 1 cut(s) | 342 |
| BsuRI | GGCC | 5 cut(s) | 35, 135, 189, 203, 305 |
| BtsCI | GGATG | 2 cut(s) | 616, 717 |
| BtsI | GCAGTG | 1 cut(s) | 637 |
| BtsIMutI | CAGTG | 2 cut(s) | 211, 637 |
| BveI | ACCTGC | 1 cut(s) | 449 |
| Cac8I | GCNNGC | 1 cut(s) | 133 |
| CaiI | CAGNNNCTG | 1 cut(s) | 482 |
| Cfr13I | GGNCC | 1 cut(s) | 397 |
| CseI | GACGC | 1 cut(s) | 89 |
| Csp6I | GTAC | 3 cut(s) | 252, 635, 680 |
| CviAII | CATG | 3 cut(s) | 562, 638, 753 |
| CviQI | GTAC | 3 cut(s) | 252, 635, 680 |
| DdeI | CTNAG | 3 cut(s) | 30, 401, 422 |
| DraI | TTTAAA | 2 cut(s) | 295, 379 |
| Eam1104I | CTCTTC | 1 cut(s) | 667 |
| EarI | CTCTTC | 1 cut(s) | 667 |
| Ecl136II | GAGCTC | 1 cut(s) | 419 |
| Eco130I | CCWWGG | 2 cut(s) | 40, 325 |
| Eco24I | GRGCYC | 1 cut(s) | 421 |
| Eco47I | GGWCC | 1 cut(s) | 397 |
| Eco53kI | GAGCTC | 1 cut(s) | 419 |
| Eco88I | CYCGRG | 1 cut(s) | 537 |
| EcoICRI | GAGCTC | 1 cut(s) | 419 |
| EcoRI | GAATTC | 1 cut(s) | 669 |
| EcoRII | CCWGG | 1 cut(s) | 705 |
| EcoT14I | CCWWGG | 2 cut(s) | 40, 325 |
| EcoT38I | GRGCYC | 1 cut(s) | 421 |
| ErhI | CCWWGG | 2 cut(s) | 40, 325 |
| FaeI | CATG | 3 cut(s) | 565, 641, 756 |
| FaqI | GGGAC | 1 cut(s) | 59 |
| FatI | CATG | 3 cut(s) | 561, 637, 752 |
| Fnu4HI | GCNGC | 4 cut(s) | 171, 174, 762, 774 |
| FokI | GGATG | 2 cut(s) | 623, 724 |
| FriOI | GRGCYC | 1 cut(s) | 421 |
| Fsp4HI | GCNGC | 4 cut(s) | 171, 174, 762, 774 |
| FspBI | CTAG | 3 cut(s) | 414, 528, 666 |
| GluI | GCNGC | 4 cut(s) | 171, 174, 762, 774 |
| GsuI | CTGGAG | 2 cut(s) | 333, 626 |
| HaeIII | GGCC | 5 cut(s) | 35, 135, 189, 203, 305 |
| HgaI | GACGC | 1 cut(s) | 89 |
| Hin1II | CATG | 3 cut(s) | 565, 641, 756 |
| HinfI | GANTC | 1 cut(s) | 92 |
| HphI | GGTGA | 3 cut(s) | 314, 344, 367 |
| Hpy166II | GTNNAC | 1 cut(s) | 635 |
| Hpy188I | TCNGA | 1 cut(s) | 11 |
| Hpy188III | TCNNGA | 5 cut(s) | 312, 414, 605, 666, 737 |
| Hpy8I | GTNNAC | 1 cut(s) | 635 |
| HpyAV | CCTTC | 3 cut(s) | 193, 295, 442 |
| HpyCH4III | ACNGT | 4 cut(s) | 52, 73, 365, 500 |
| HpyCH4V | TGCA | 5 cut(s) | 62, 505, 614, 694, 723 |
| HpyF10VI | GCNNNNNNNGC | 4 cut(s) | 132, 179, 758, 770 |
| HpyF3I | CTNAG | 3 cut(s) | 30, 401, 422 |
| Hsp92II | CATG | 3 cut(s) | 565, 641, 756 |
| LguI | GCTCTTC | 1 cut(s) | 667 |
| LmnI | GCTCC | 1 cut(s) | 586 |
| Lsp1109I | GCAGC | 3 cut(s) | 185, 748, 760 |
| LweI | GCATC | 5 cut(s) | 6, 106, 135, 514, 601 |
| MaeI | CTAG | 3 cut(s) | 414, 528, 666 |
| MaeIII | GTNAC | 1 cut(s) | 407 |
| MboII | GAAGA | 3 cut(s) | 347, 562, 654 |
| MhlI | GDGCHC | 1 cut(s) | 421 |
| MluCI | AATT | 7 cut(s) | 270, 358, 374, 391, 492, 545, 669 |
| MnlI | CCTC | 6 cut(s) | 25, 323, 450, 533, 601, 736 |
| MseI | TTAA | 5 cut(s) | 294, 369, 378, 431, 732 |
| MspR9I | CCNGG | 1 cut(s) | 707 |
| MvaI | CCWGG | 1 cut(s) | 707 |
| MwoI | GCNNNNNNNGC | 4 cut(s) | 132, 179, 758, 770 |
| NlaIII | CATG | 3 cut(s) | 565, 641, 756 |
| NspI | RCATGY | 1 cut(s) | 756 |
| PaeR7I | CTCGAG | 1 cut(s) | 537 |
| PciSI | GCTCTTC | 1 cut(s) | 667 |
| PfeI | GAWTC | 1 cut(s) | 92 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 712 |
| PkrI | GCNGC | 4 cut(s) | 172, 175, 763, 775 |
| PshBI | ATTAAT | 1 cut(s) | 732 |
| PsiI | TTATAA | 1 cut(s) | 155 |
| Psp124BI | GAGCTC | 1 cut(s) | 421 |
| Psp6I | CCWGG | 1 cut(s) | 705 |
| PspGI | CCWGG | 1 cut(s) | 705 |
| PspPI | GGNCC | 1 cut(s) | 397 |
| PspXI | VCTCGAGB | 1 cut(s) | 537 |
| PstNI | CAGNNNCTG | 1 cut(s) | 482 |
| RsaI | GTAC | 3 cut(s) | 253, 636, 681 |
| RsaNI | GTAC | 3 cut(s) | 252, 635, 680 |
| SacI | GAGCTC | 1 cut(s) | 421 |
| SapI | GCTCTTC | 1 cut(s) | 667 |
| SaqAI | TTAA | 5 cut(s) | 294, 369, 378, 431, 732 |
| SatI | GCNGC | 4 cut(s) | 171, 174, 762, 774 |
| Sau96I | GGNCC | 1 cut(s) | 397 |
| ScrFI | CCNGG | 1 cut(s) | 707 |
| SduI | GDGCHC | 1 cut(s) | 421 |
| SfaNI | GCATC | 5 cut(s) | 6, 106, 135, 514, 601 |
| Sfr274I | CTCGAG | 1 cut(s) | 537 |
| SinI | GGWCC | 1 cut(s) | 397 |
| SlaI | CTCGAG | 1 cut(s) | 537 |
| SmlI | CTYRAG | 1 cut(s) | 537 |
| SmoI | CTYRAG | 1 cut(s) | 537 |
| SpeI | ACTAGT | 1 cut(s) | 527 |
| Sse9I | AATT | 7 cut(s) | 270, 358, 374, 391, 492, 545, 669 |
| SsiI | CCGC | 2 cut(s) | 148, 171 |
| SspMI | CTAG | 3 cut(s) | 414, 528, 666 |
| SstI | GAGCTC | 1 cut(s) | 421 |
| StyD4I | CCNGG | 1 cut(s) | 705 |
| StyI | CCWWGG | 2 cut(s) | 40, 325 |
| TaaI | ACNGT | 4 cut(s) | 52, 73, 365, 500 |
| TaqI | TCGA | 2 cut(s) | 342, 538 |
| TasI | AATT | 7 cut(s) | 270, 358, 374, 391, 492, 545, 669 |
| TatI | WGTACW | 2 cut(s) | 634, 679 |
| TauI | GCSGC | 1 cut(s) | 173 |
| TfiI | GAWTC | 1 cut(s) | 92 |
| Tru1I | TTAA | 5 cut(s) | 294, 369, 378, 431, 732 |
| Tru9I | TTAA | 5 cut(s) | 294, 369, 378, 431, 732 |
| TscAI | CASTG | 2 cut(s) | 211, 637 |
| TseI | GCWGC | 3 cut(s) | 173, 761, 773 |
| TspDTI | ATGAA | 1 cut(s) | 174 |
| TspRI | CASTG | 2 cut(s) | 211, 637 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 712 |
| VpaK11BI | GGWCC | 1 cut(s) | 397 |
| VspI | ATTAAT | 1 cut(s) | 732 |
| XapI | RAATTY | 2 cut(s) | 545, 669 |
| XbaI | TCTAGA | 2 cut(s) | 413, 665 |
| XceI | RCATGY | 1 cut(s) | 756 |
| XhoI | CTCGAG | 1 cut(s) | 537 |
| XspI | CTAG | 3 cut(s) | 414, 528, 666 |