Belongs to the heat shock protein 70 family
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 2 cut(s) | 1236, 1617 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 29 |
| AccBSI | CCGCTC | 1 cut(s) | 848 |
| AciI | CCGC | 7 cut(s) | 540, 546, 588, 846, 1118, 1485, 1730 |
| AclI | AACGTT | 1 cut(s) | 597 |
| AclWI | GGATC | 3 cut(s) | 424, 945, 1039 |
| AcsI | RAATTY | 4 cut(s) | 239, 347, 660, 917 |
| AcuI | CTGAAG | 1 cut(s) | 378 |
| AfaI | GTAC | 5 cut(s) | 625, 741, 1140, 1390, 1595 |
| AfeI | AGCGCT | 1 cut(s) | 224 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 29, 258 |
| AflIII | ACRYGT | 1 cut(s) | 1596 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 1367, 1399 |
| AleI | CACNNNNGTG | 1 cut(s) | 81 |
| AloI | GAACNNNNNNTCC | 2 cut(s) | 582, 614 |
| AluBI | AGCT | 8 cut(s) | 98, 677, 683, 1090, 1145, 1399, 1804, 1813 |
| AluI | AGCT | 8 cut(s) | 98, 677, 683, 1090, 1145, 1399, 1804, 1813 |
| Alw26I | GTCTC | 1 cut(s) | 1486 |
| AlwI | GGATC | 3 cut(s) | 424, 945, 1039 |
| Aor51HI | AGCGCT | 1 cut(s) | 224 |
| AoxI | GGCC | 2 cut(s) | 278, 477 |
| ApeKI | GCWGC | 4 cut(s) | 170, 353, 542, 1145 |
| ApoI | RAATTY | 4 cut(s) | 239, 347, 660, 917 |
| Asp700I | GAANNNNTTC | 2 cut(s) | 734, 835 |
| AspLEI | GCGC | 1 cut(s) | 225 |
| AspS9I | GGNCC | 1 cut(s) | 20 |
| AsuHPI | GGTGA | 5 cut(s) | 176, 682, 716, 739, 1519 |
| AvaII | GGWCC | 1 cut(s) | 20 |
| BbsI | GAAGAC | 1 cut(s) | 1713 |
| BbvI | GCAGC | 4 cut(s) | 182, 340, 529, 1132 |
| BccI | CCATC | 3 cut(s) | 1283, 1567, 1663 |
| BcgI | CGANNNNNNTGC | 4 cut(s) | 22, 56, 62, 96 |
| BciVI | GTATCC | 2 cut(s) | 196, 1340 |
| BclI | TGATCA | 1 cut(s) | 103 |
| BcoDI | GTCTC | 1 cut(s) | 1486 |
| BfaI | CTAG | 1 cut(s) | 1038 |
| BfmI | CTRYAG | 1 cut(s) | 678 |
| BfoI | RGCGCY | 1 cut(s) | 226 |
| BfuI | GTATCC | 2 cut(s) | 196, 1340 |
| BglII | AGATCT | 1 cut(s) | 1069 |
| BisI | GCNGC | 5 cut(s) | 171, 354, 543, 546, 1146 |
| BlsI | GCNGC | 5 cut(s) | 172, 355, 544, 547, 1147 |
| Bme18I | GGWCC | 1 cut(s) | 20 |
| BmgT120I | GGNCC | 1 cut(s) | 20 |
| BmiI | GGNNCC | 2 cut(s) | 21, 960 |
| BpiI | GAAGAC | 1 cut(s) | 1713 |
| BpmI | CTGGAG | 1 cut(s) | 705 |
| BpuEI | CTTGAG | 1 cut(s) | 629 |
| BsaBI | GATNNNNATC | 3 cut(s) | 32, 693, 1523 |
| BsaJI | CCNNGG | 2 cut(s) | 40, 1488 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 29, 258 |
| Bse118I | RCCGGY | 1 cut(s) | 491 |
| Bse1I | ACTGG | 1 cut(s) | 1333 |
| Bse3DI | GCAATG | 1 cut(s) | 549 |
| Bse8I | GATNNNNATC | 3 cut(s) | 32, 693, 1523 |
| BseDI | CCNNGG | 2 cut(s) | 40, 1488 |
| BseGI | GGATG | 6 cut(s) | 493, 513, 1207, 1275, 1407, 1732 |
| BseJI | GATNNNNATC | 3 cut(s) | 32, 693, 1523 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 29, 258 |
| BseMI | GCAATG | 1 cut(s) | 549 |
| BseMII | CTCAG | 1 cut(s) | 1796 |
| BseNI | ACTGG | 1 cut(s) | 1333 |
| BseXI | GCAGC | 4 cut(s) | 182, 340, 529, 1132 |
| BsgI | GTGCAG | 2 cut(s) | 1302, 1846 |
| Bsh1285I | CGRYCG | 2 cut(s) | 82, 1137 |
| BshFI | GGCC | 2 cut(s) | 280, 479 |
| BsiEI | CGRYCG | 2 cut(s) | 82, 1137 |
| BsiSI | CCGG | 1 cut(s) | 492 |
| BslFI | GGGAC | 2 cut(s) | 1244, 1815 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 29, 258 |
| BsmAI | GTCTC | 1 cut(s) | 1486 |
| BsmFI | GGGAC | 2 cut(s) | 1244, 1815 |
| BsnI | GGCC | 2 cut(s) | 280, 479 |
| Bsp143I | GATC | 9 cut(s) | 103, 228, 416, 610, 937, 1031, 1069, 1295, 1518 |
| BspACI | CCGC | 7 cut(s) | 540, 546, 588, 846, 1118, 1485, 1730 |
| BspANI | GGCC | 2 cut(s) | 280, 479 |
| BspCNI | CTCAG | 1 cut(s) | 1797 |
| BspHI | TCATGA | 1 cut(s) | 1009 |
| BspLI | GGNNCC | 2 cut(s) | 21, 960 |
| BspPI | GGATC | 3 cut(s) | 424, 945, 1039 |
| BsrBI | CCGCTC | 1 cut(s) | 848 |
| BsrDI | GCAATG | 1 cut(s) | 549 |
| BsrFI | RCCGGY | 1 cut(s) | 491 |
| BsrI | ACTGG | 1 cut(s) | 1333 |
| BssAI | RCCGGY | 1 cut(s) | 491 |
| BssECI | CCNNGG | 2 cut(s) | 40, 1488 |
| BssMI | GATC | 9 cut(s) | 103, 228, 416, 610, 937, 1031, 1069, 1295, 1518 |
| Bst4CI | ACNGT | 9 cut(s) | 80, 126, 208, 424, 445, 583, 1138, 1309, 1678 |
| Bst6I | CTCTTC | 2 cut(s) | 354, 648 |
| BstAPI | GCANNNNNTGC | 2 cut(s) | 1130, 1551 |
| BstDEI | CTNAG | 2 cut(s) | 794, 1805 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 256 |
| BstF5I | GGATG | 6 cut(s) | 493, 513, 1207, 1275, 1407, 1732 |
| BstH2I | RGCGCY | 1 cut(s) | 226 |
| BstHHI | GCGC | 1 cut(s) | 225 |
| BstKTI | GATC | 9 cut(s) | 106, 231, 419, 613, 940, 1034, 1072, 1298, 1521 |
| BstMAI | GTCTC | 1 cut(s) | 1486 |
| BstMBI | GATC | 9 cut(s) | 103, 228, 416, 610, 937, 1031, 1069, 1295, 1518 |
| BstMCI | CGRYCG | 2 cut(s) | 82, 1137 |
| BstMWI | GCNNNNNNNGC | 5 cut(s) | 551, 1130, 1551, 1640, 1810 |
| BstNSI | RCATGY | 1 cut(s) | 1600 |
| BstSFI | CTRYAG | 1 cut(s) | 678 |
| BstV1I | GCAGC | 4 cut(s) | 182, 340, 529, 1132 |
| BstV2I | GAAGAC | 1 cut(s) | 1713 |
| BstX2I | RGATCY | 3 cut(s) | 937, 1031, 1069 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 1669 |
| BstYI | RGATCY | 3 cut(s) | 937, 1031, 1069 |
| BsuI | GTATCC | 2 cut(s) | 196, 1340 |
| BsuRI | GGCC | 2 cut(s) | 280, 479 |
| BtgZI | GCGATG | 1 cut(s) | 261 |
| BtsCI | GGATG | 6 cut(s) | 493, 513, 1207, 1275, 1407, 1732 |
| BtsIMutI | CAGTG | 1 cut(s) | 204 |
| CciI | TCATGA | 1 cut(s) | 1009 |
| CfoI | GCGC | 1 cut(s) | 225 |
| Cfr10I | RCCGGY | 1 cut(s) | 491 |
| Cfr13I | GGNCC | 1 cut(s) | 20 |
| Csp6I | GTAC | 5 cut(s) | 624, 740, 1139, 1389, 1594 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 274, 309 |
| CviAII | CATG | 9 cut(s) | 57, 268, 313, 373, 761, 934, 1010, 1326, 1597 |
| CviQI | GTAC | 5 cut(s) | 624, 740, 1139, 1389, 1594 |
| DdeI | CTNAG | 2 cut(s) | 794, 1805 |
| DpnI | GATC | 9 cut(s) | 105, 230, 418, 612, 939, 1033, 1071, 1297, 1520 |
| DpnII | GATC | 9 cut(s) | 103, 228, 416, 610, 937, 1031, 1069, 1295, 1518 |
| Eam1104I | CTCTTC | 2 cut(s) | 354, 648 |
| EarI | CTCTTC | 2 cut(s) | 354, 648 |
| EciI | GGCGGA | 2 cut(s) | 603, 1474 |
| Eco32I | GATATC | 4 cut(s) | 366, 772, 928, 1438 |
| Eco47I | GGWCC | 1 cut(s) | 20 |
| Eco47III | AGCGCT | 1 cut(s) | 224 |
| Eco57I | CTGAAG | 1 cut(s) | 378 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 256 |
| EcoRV | GATATC | 4 cut(s) | 366, 772, 928, 1438 |
| FaeI | CATG | 9 cut(s) | 60, 271, 316, 376, 764, 937, 1013, 1329, 1600 |
| FaqI | GGGAC | 2 cut(s) | 1244, 1815 |
| FatI | CATG | 9 cut(s) | 56, 267, 312, 372, 760, 933, 1009, 1325, 1596 |
| FauNDI | CATATG | 2 cut(s) | 1126, 1245 |
| FbaI | TGATCA | 1 cut(s) | 103 |
| Fnu4HI | GCNGC | 5 cut(s) | 171, 354, 543, 546, 1146 |
| FokI | GGATG | 6 cut(s) | 500, 520, 1214, 1262, 1394, 1739 |
| Fsp4HI | GCNGC | 5 cut(s) | 171, 354, 543, 546, 1146 |
| FspBI | CTAG | 1 cut(s) | 1038 |
| GlaI | GCGC | 1 cut(s) | 224 |
| GluI | GCNGC | 5 cut(s) | 171, 354, 543, 546, 1146 |
| GsuI | CTGGAG | 1 cut(s) | 705 |
| HaeII | RGCGCY | 1 cut(s) | 226 |
| HaeIII | GGCC | 2 cut(s) | 280, 479 |
| HapII | CCGG | 1 cut(s) | 492 |
| HhaI | GCGC | 1 cut(s) | 225 |
| Hin1II | CATG | 9 cut(s) | 60, 271, 316, 376, 764, 937, 1013, 1329, 1600 |
| Hin6I | GCGC | 1 cut(s) | 223 |
| HinP1I | GCGC | 1 cut(s) | 223 |
| HincII | GTYRAC | 1 cut(s) | 325 |
| HindII | GTYRAC | 1 cut(s) | 325 |
| HinfI | GANTC | 8 cut(s) | 178, 376, 730, 869, 1041, 1470, 1719, 1768 |
| HpaI | GTTAAC | 1 cut(s) | 325 |
| HpaII | CCGG | 1 cut(s) | 492 |
| HphI | GGTGA | 5 cut(s) | 176, 682, 716, 739, 1519 |
| Hpy166II | GTNNAC | 2 cut(s) | 325, 967 |
| Hpy188I | TCNGA | 3 cut(s) | 44, 416, 1603 |
| Hpy188III | TCNNGA | 8 cut(s) | 563, 646, 751, 1010, 1109, 1184, 1433, 1474 |
| Hpy8I | GTNNAC | 2 cut(s) | 325, 967 |
| Hpy99I | CGWCG | 2 cut(s) | 635, 1313 |
| HpyAV | CCTTC | 1 cut(s) | 650 |
| HpyCH4III | ACNGT | 9 cut(s) | 80, 126, 208, 424, 445, 583, 1138, 1309, 1678 |
| HpyCH4IV | ACGT | 4 cut(s) | 132, 472, 597, 633 |
| HpyF10VI | GCNNNNNNNGC | 5 cut(s) | 551, 1130, 1551, 1640, 1810 |
| HpyF3I | CTNAG | 2 cut(s) | 794, 1805 |
| HpySE526I | ACGT | 4 cut(s) | 132, 472, 597, 633 |
| Hsp92II | CATG | 9 cut(s) | 60, 271, 316, 376, 764, 937, 1013, 1329, 1600 |
| HspAI | GCGC | 1 cut(s) | 223 |
| Ksp22I | TGATCA | 1 cut(s) | 103 |
| KspAI | GTTAAC | 1 cut(s) | 325 |
| Kzo9I | GATC | 9 cut(s) | 103, 228, 416, 610, 937, 1031, 1069, 1295, 1518 |
| LmnI | GCTCC | 2 cut(s) | 958, 1087 |
| Lsp1109I | GCAGC | 4 cut(s) | 182, 340, 529, 1132 |
| MaeI | CTAG | 1 cut(s) | 1038 |
| MaeII | ACGT | 4 cut(s) | 132, 472, 597, 633 |
| MaeIII | GTNAC | 5 cut(s) | 263, 439, 1204, 1300, 1312 |
| MalI | GATC | 9 cut(s) | 105, 230, 418, 612, 939, 1033, 1071, 1297, 1520 |
| MbiI | CCGCTC | 1 cut(s) | 848 |
| MboI | GATC | 9 cut(s) | 103, 228, 416, 610, 937, 1031, 1069, 1295, 1518 |
| MflI | RGATCY | 3 cut(s) | 937, 1031, 1069 |
| MluCI | AATT | 8 cut(s) | 159, 239, 347, 498, 660, 917, 1149, 1563 |
| MmeI | TCCRAC | 2 cut(s) | 47, 1179 |
| MroXI | GAANNNNTTC | 2 cut(s) | 734, 835 |
| MseI | TTAA | 4 cut(s) | 158, 324, 384, 1077 |
| MslI | CAYNNNNRTG | 2 cut(s) | 81, 1324 |
| MspA1I | CMGCKG | 2 cut(s) | 542, 683 |
| MspI | CCGG | 1 cut(s) | 492 |
| MwoI | GCNNNNNNNGC | 5 cut(s) | 551, 1130, 1551, 1640, 1810 |
| NdeI | CATATG | 2 cut(s) | 1126, 1245 |
| NdeII | GATC | 9 cut(s) | 103, 228, 416, 610, 937, 1031, 1069, 1295, 1518 |
| NlaIII | CATG | 9 cut(s) | 60, 271, 316, 376, 764, 937, 1013, 1329, 1600 |
| NlaIV | GGNNCC | 2 cut(s) | 21, 960 |
| NmeAIII | GCCGAG | 1 cut(s) | 1513 |
| NmuCI | GTSAC | 3 cut(s) | 263, 1204, 1300 |
| NspI | RCATGY | 1 cut(s) | 1600 |
| OliI | CACNNNNGTG | 1 cut(s) | 81 |
| PagI | TCATGA | 1 cut(s) | 1009 |
| PciI | ACATGT | 1 cut(s) | 1596 |
| PdmI | GAANNNNTTC | 2 cut(s) | 734, 835 |
| PfeI | GAWTC | 8 cut(s) | 178, 376, 730, 869, 1041, 1470, 1719, 1768 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 29 |
| PkrI | GCNGC | 5 cut(s) | 172, 355, 544, 547, 1147 |
| PscI | ACATGT | 1 cut(s) | 1596 |
| PsiI | TTATAA | 2 cut(s) | 1236, 1617 |
| Psp1406I | AACGTT | 1 cut(s) | 597 |
| PspN4I | GGNNCC | 2 cut(s) | 21, 960 |
| PspPI | GGNCC | 1 cut(s) | 20 |
| PsuI | RGATCY | 3 cut(s) | 937, 1031, 1069 |
| PvuII | CAGCTG | 1 cut(s) | 683 |
| RsaI | GTAC | 5 cut(s) | 625, 741, 1140, 1390, 1595 |
| RsaNI | GTAC | 5 cut(s) | 624, 740, 1139, 1389, 1594 |
| RseI | CAYNNNNRTG | 2 cut(s) | 81, 1324 |
| SaqAI | TTAA | 4 cut(s) | 158, 324, 384, 1077 |
| SatI | GCNGC | 5 cut(s) | 171, 354, 543, 546, 1146 |
| Sau3AI | GATC | 9 cut(s) | 103, 228, 416, 610, 937, 1031, 1069, 1295, 1518 |
| Sau96I | GGNCC | 1 cut(s) | 20 |
| SfcI | CTRYAG | 1 cut(s) | 678 |
| SinI | GGWCC | 1 cut(s) | 20 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 81, 1324 |
| SmlI | CTYRAG | 1 cut(s) | 644 |
| SmoI | CTYRAG | 1 cut(s) | 644 |
| Sse9I | AATT | 8 cut(s) | 159, 239, 347, 498, 660, 917, 1149, 1563 |
| SsiI | CCGC | 7 cut(s) | 540, 546, 588, 846, 1118, 1485, 1730 |
| SspI | AATATT | 1 cut(s) | 514 |
| SspMI | CTAG | 1 cut(s) | 1038 |
| TaaI | ACNGT | 9 cut(s) | 80, 126, 208, 424, 445, 583, 1138, 1309, 1678 |
| TaiI | ACGT | 4 cut(s) | 135, 475, 600, 636 |
| TaqI | TCGA | 5 cut(s) | 36, 419, 630, 1578, 1815 |
| TasI | AATT | 8 cut(s) | 159, 239, 347, 498, 660, 917, 1149, 1563 |
| TatI | WGTACW | 1 cut(s) | 1593 |
| TauI | GCSGC | 1 cut(s) | 548 |
| TfiI | GAWTC | 8 cut(s) | 178, 376, 730, 869, 1041, 1470, 1719, 1768 |
| Tru1I | TTAA | 4 cut(s) | 158, 324, 384, 1077 |
| Tru9I | TTAA | 4 cut(s) | 158, 324, 384, 1077 |
| TscAI | CASTG | 1 cut(s) | 211 |
| TseFI | GTSAC | 3 cut(s) | 263, 1204, 1300 |
| TseI | GCWGC | 4 cut(s) | 170, 353, 542, 1145 |
| Tsp45I | GTSAC | 3 cut(s) | 263, 1204, 1300 |
| TspDTI | ATGAA | 6 cut(s) | 284, 524, 777, 998, 1128, 1670 |
| TspGWI | ACGGA | 3 cut(s) | 509, 1400, 1643 |
| TspRI | CASTG | 1 cut(s) | 211 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 29 |
| VpaK11BI | GGWCC | 1 cut(s) | 20 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 256 |
| XapI | RAATTY | 4 cut(s) | 239, 347, 660, 917 |
| XceI | RCATGY | 1 cut(s) | 1600 |
| XmnI | GAANNNNTTC | 2 cut(s) | 734, 835 |
| XspI | CTAG | 1 cut(s) | 1038 |