Heat shock cognate 70 kDa
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccII | CGCG | 3 cut(s) | 480, 918, 920 |
| AciI | CCGC | 2 cut(s) | 397, 1454 |
| AclI | AACGTT | 1 cut(s) | 1354 |
| AclWI | GGATC | 1 cut(s) | 430 |
| AcoI | YGGCCR | 1 cut(s) | 281 |
| AcsI | RAATTY | 4 cut(s) | 242, 736, 1047, 1466 |
| AcuI | CTGAAG | 2 cut(s) | 384, 1159 |
| AfaI | GTAC | 2 cut(s) | 606, 1240 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 1543 |
| AflIII | ACRYGT | 1 cut(s) | 1524 |
| AhlI | ACTAGT | 1 cut(s) | 1247 |
| AjnI | CCWGG | 1 cut(s) | 159 |
| AjuI | GAANNNNNNNTTGG | 4 cut(s) | 402, 434, 755, 787 |
| AleI | CACNNNNGTG | 1 cut(s) | 705 |
| AluBI | AGCT | 8 cut(s) | 157, 277, 497, 934, 1096, 1142, 1151, 1463 |
| AluI | AGCT | 8 cut(s) | 157, 277, 497, 934, 1096, 1142, 1151, 1463 |
| Alw21I | GWGCWC | 2 cut(s) | 159, 361 |
| Alw26I | GTCTC | 2 cut(s) | 687, 832 |
| AlwI | GGATC | 1 cut(s) | 430 |
| AoxI | GGCC | 5 cut(s) | 281, 483, 511, 565, 1534 |
| ApeKI | GCWGC | 7 cut(s) | 114, 173, 274, 548, 551, 1139, 1151 |
| ApoI | RAATTY | 4 cut(s) | 242, 736, 1047, 1466 |
| AseI | ATTAAT | 1 cut(s) | 534 |
| AspLEI | GCGC | 3 cut(s) | 480, 920, 1511 |
| AspS9I | GGNCC | 3 cut(s) | 120, 302, 451 |
| AsuHPI | GGTGA | 9 cut(s) | 671, 692, 722, 745, 985, 1204, 1297, 1394, 1469 |
| AvaII | GGWCC | 3 cut(s) | 120, 302, 451 |
| BaeGI | GKGCMC | 1 cut(s) | 44 |
| BaeI | ACNNNNGTAYC | 2 cut(s) | 243, 276 |
| BalI | TGGCCA | 1 cut(s) | 283 |
| BanII | GRGCYC | 1 cut(s) | 159 |
| Bbv12I | GWGCWC | 2 cut(s) | 159, 361 |
| BbvCI | CCTCAGC | 1 cut(s) | 1518 |
| BbvI | GCAGC | 7 cut(s) | 126, 185, 286, 560, 563, 1138, 1151 |
| BccI | CCATC | 3 cut(s) | 136, 656, 1559 |
| BciT130I | CCWGG | 1 cut(s) | 161 |
| BclI | TGATCA | 1 cut(s) | 444 |
| BcoDI | GTCTC | 2 cut(s) | 687, 832 |
| BcuI | ACTAGT | 1 cut(s) | 1247 |
| BfaI | CTAG | 2 cut(s) | 1044, 1248 |
| BfmI | CTRYAG | 4 cut(s) | 507, 546, 1070, 1152 |
| BfoI | RGCGCY | 1 cut(s) | 1512 |
| BglII | AGATCT | 2 cut(s) | 369, 1326 |
| BisI | GCNGC | 7 cut(s) | 115, 174, 275, 549, 552, 1140, 1152 |
| BlsI | GCNGC | 7 cut(s) | 116, 175, 276, 550, 553, 1141, 1153 |
| BmcAI | AGTACT | 1 cut(s) | 1240 |
| Bme1390I | CCNGG | 1 cut(s) | 161 |
| Bme18I | GGWCC | 3 cut(s) | 120, 302, 451 |
| BmgT120I | GGNCC | 3 cut(s) | 120, 302, 451 |
| BmiI | GGNNCC | 3 cut(s) | 453, 966, 1449 |
| BmrFI | CCNGG | 1 cut(s) | 161 |
| BmsI | GCATC | 8 cut(s) | 208, 510, 513, 892, 979, 1384, 1480, 1593 |
| BpmI | CTGGAG | 1 cut(s) | 711 |
| Bpu10I | CCTNAGC | 1 cut(s) | 1518 |
| BpuEI | CTTGAG | 2 cut(s) | 1067, 1525 |
| Bsa29I | ATCGAT | 1 cut(s) | 33 |
| BsaBI | GATNNNNATC | 1 cut(s) | 1553 |
| BsaJI | CCNNGG | 2 cut(s) | 378, 703 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 1543 |
| Bse1I | ACTGG | 1 cut(s) | 968 |
| Bse3DI | GCAATG | 1 cut(s) | 555 |
| Bse8I | GATNNNNATC | 1 cut(s) | 1553 |
| BseBI | CCWGG | 1 cut(s) | 161 |
| BseCI | ATCGAT | 1 cut(s) | 33 |
| BseDI | CCNNGG | 2 cut(s) | 378, 703 |
| BseGI | GGATG | 5 cut(s) | 374, 643, 781, 994, 1213 |
| BseJI | GATNNNNATC | 1 cut(s) | 1553 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 1543 |
| BseMI | GCAATG | 1 cut(s) | 555 |
| BseMII | CTCAG | 2 cut(s) | 144, 1509 |
| BseNI | ACTGG | 1 cut(s) | 968 |
| BseRI | GAGGAG | 2 cut(s) | 764, 850 |
| BseSI | GKGCMC | 1 cut(s) | 44 |
| BseXI | GCAGC | 7 cut(s) | 126, 185, 286, 560, 563, 1138, 1151 |
| BsgI | GTGCAG | 1 cut(s) | 1158 |
| Bsh1236I | CGCG | 3 cut(s) | 480, 918, 920 |
| BshFI | GGCC | 5 cut(s) | 283, 485, 513, 567, 1536 |
| BshVI | ATCGAT | 1 cut(s) | 33 |
| BsiHKAI | GWGCWC | 2 cut(s) | 159, 361 |
| BsiSI | CCGG | 1 cut(s) | 1445 |
| BslFI | GGGAC | 1 cut(s) | 437 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 1543 |
| BsmAI | GTCTC | 2 cut(s) | 687, 832 |
| BsmFI | GGGAC | 1 cut(s) | 437 |
| BsmI | GAATGC | 2 cut(s) | 409, 442 |
| BsnI | GGCC | 5 cut(s) | 283, 485, 513, 567, 1536 |
| Bsp1286I | GDGCHC | 3 cut(s) | 44, 159, 361 |
| Bsp143I | GATC | 6 cut(s) | 34, 231, 369, 422, 444, 1326 |
| Bsp19I | CCATGG | 1 cut(s) | 378 |
| BspACI | CCGC | 2 cut(s) | 397, 1454 |
| BspANI | GGCC | 5 cut(s) | 283, 485, 513, 567, 1536 |
| BspCNI | CTCAG | 2 cut(s) | 145, 1510 |
| BspDI | ATCGAT | 1 cut(s) | 33 |
| BspFNI | CGCG | 3 cut(s) | 480, 918, 920 |
| BspHI | TCATGA | 2 cut(s) | 189, 1432 |
| BspLI | GGNNCC | 3 cut(s) | 453, 966, 1449 |
| BspMAI | CTGCAG | 3 cut(s) | 511, 550, 1156 |
| BspPI | GGATC | 1 cut(s) | 430 |
| BspQI | GCTCTTC | 1 cut(s) | 1045 |
| BsrDI | GCAATG | 1 cut(s) | 555 |
| BsrI | ACTGG | 1 cut(s) | 968 |
| BssECI | CCNNGG | 2 cut(s) | 378, 703 |
| BssMI | GATC | 6 cut(s) | 34, 231, 369, 422, 444, 1326 |
| BssT1I | CCWWGG | 2 cut(s) | 378, 703 |
| Bst2UI | CCWGG | 1 cut(s) | 161 |
| Bst4CI | ACNGT | 5 cut(s) | 259, 430, 451, 1339, 1562 |
| Bst6I | CTCTTC | 2 cut(s) | 360, 1045 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 557 |
| BstC8I | GCNNGC | 2 cut(s) | 499, 511 |
| BstDEI | CTNAG | 4 cut(s) | 153, 800, 1330, 1518 |
| BstDSI | CCRYGG | 1 cut(s) | 378 |
| BstF5I | GGATG | 5 cut(s) | 374, 643, 781, 994, 1213 |
| BstFNI | CGCG | 3 cut(s) | 480, 918, 920 |
| BstH2I | RGCGCY | 1 cut(s) | 1512 |
| BstHHI | GCGC | 3 cut(s) | 480, 920, 1511 |
| BstKTI | GATC | 6 cut(s) | 37, 234, 372, 425, 447, 1329 |
| BstMAI | GTCTC | 2 cut(s) | 687, 832 |
| BstMBI | GATC | 6 cut(s) | 34, 231, 369, 422, 444, 1326 |
| BstMWI | GCNNNNNNNGC | 5 cut(s) | 280, 557, 1136, 1148, 1460 |
| BstNI | CCWGG | 1 cut(s) | 161 |
| BstNSI | RCATGY | 3 cut(s) | 23, 822, 1528 |
| BstSCI | CCNGG | 1 cut(s) | 159 |
| BstSFI | CTRYAG | 4 cut(s) | 507, 546, 1070, 1152 |
| BstSLI | GKGCMC | 1 cut(s) | 44 |
| BstUI | CGCG | 3 cut(s) | 480, 918, 920 |
| BstV1I | GCAGC | 7 cut(s) | 126, 185, 286, 560, 563, 1138, 1151 |
| BstX2I | RGATCY | 2 cut(s) | 369, 1326 |
| BstYI | RGATCY | 2 cut(s) | 369, 1326 |
| Bsu15I | ATCGAT | 1 cut(s) | 33 |
| BsuRI | GGCC | 5 cut(s) | 283, 485, 513, 567, 1536 |
| BsuTUI | ATCGAT | 1 cut(s) | 33 |
| BtgI | CCRYGG | 1 cut(s) | 378 |
| BtgZI | GCGATG | 1 cut(s) | 1407 |
| BtsCI | GGATG | 5 cut(s) | 374, 643, 781, 994, 1213 |
| BtsI | GCAGTG | 3 cut(s) | 447, 504, 684 |
| BtsIMutI | CAGTG | 9 cut(s) | 144, 253, 447, 504, 684, 787, 975, 1183, 1567 |
| Cac8I | GCNNGC | 2 cut(s) | 499, 511 |
| CciI | TCATGA | 2 cut(s) | 189, 1432 |
| CfoI | GCGC | 3 cut(s) | 480, 920, 1511 |
| Cfr13I | GGNCC | 3 cut(s) | 120, 302, 451 |
| ClaI | ATCGAT | 1 cut(s) | 33 |
| Csp6I | GTAC | 2 cut(s) | 605, 1239 |
| CviQI | GTAC | 2 cut(s) | 605, 1239 |
| DdeI | CTNAG | 4 cut(s) | 153, 800, 1330, 1518 |
| DpnI | GATC | 6 cut(s) | 36, 233, 371, 424, 446, 1328 |
| DpnII | GATC | 6 cut(s) | 34, 231, 369, 422, 444, 1326 |
| EaeI | YGGCCR | 1 cut(s) | 281 |
| Eam1104I | CTCTTC | 2 cut(s) | 360, 1045 |
| EarI | CTCTTC | 2 cut(s) | 360, 1045 |
| Ecl136II | GAGCTC | 1 cut(s) | 157 |
| Eco130I | CCWWGG | 2 cut(s) | 378, 703 |
| Eco147I | AGGCCT | 1 cut(s) | 513 |
| Eco24I | GRGCYC | 1 cut(s) | 159 |
| Eco47I | GGWCC | 3 cut(s) | 120, 302, 451 |
| Eco53kI | GAGCTC | 1 cut(s) | 157 |
| Eco57I | CTGAAG | 2 cut(s) | 384, 1159 |
| EcoICRI | GAGCTC | 1 cut(s) | 157 |
| EcoO109I | RGGNCCY | 1 cut(s) | 302 |
| EcoRI | GAATTC | 1 cut(s) | 1047 |
| EcoRII | CCWGG | 1 cut(s) | 159 |
| EcoT14I | CCWWGG | 2 cut(s) | 378, 703 |
| EcoT22I | ATGCAT | 1 cut(s) | 820 |
| EcoT38I | GRGCYC | 1 cut(s) | 159 |
| ErhI | CCWWGG | 2 cut(s) | 378, 703 |
| FaqI | GGGAC | 1 cut(s) | 437 |
| FauI | CCCGC | 2 cut(s) | 390, 1447 |
| FbaI | TGATCA | 1 cut(s) | 444 |
| Fnu4HI | GCNGC | 7 cut(s) | 115, 174, 275, 549, 552, 1140, 1152 |
| FokI | GGATG | 5 cut(s) | 361, 650, 788, 1001, 1220 |
| FriOI | GRGCYC | 1 cut(s) | 159 |
| Fsp4HI | GCNGC | 7 cut(s) | 115, 174, 275, 549, 552, 1140, 1152 |
| FspBI | CTAG | 2 cut(s) | 1044, 1248 |
| GlaI | GCGC | 3 cut(s) | 479, 919, 1510 |
| GluI | GCNGC | 7 cut(s) | 115, 174, 275, 549, 552, 1140, 1152 |
| GsuI | CTGGAG | 1 cut(s) | 711 |
| HaeII | RGCGCY | 1 cut(s) | 1512 |
| HaeIII | GGCC | 5 cut(s) | 283, 485, 513, 567, 1536 |
| HapII | CCGG | 1 cut(s) | 1445 |
| HhaI | GCGC | 3 cut(s) | 480, 920, 1511 |
| Hin6I | GCGC | 3 cut(s) | 478, 918, 1509 |
| HinP1I | GCGC | 3 cut(s) | 478, 918, 1509 |
| HincII | GTYRAC | 1 cut(s) | 810 |
| HindII | GTYRAC | 1 cut(s) | 810 |
| HinfI | GANTC | 2 cut(s) | 30, 1288 |
| HpaI | GTTAAC | 1 cut(s) | 810 |
| HpaII | CCGG | 1 cut(s) | 1445 |
| HphI | GGTGA | 9 cut(s) | 671, 692, 722, 745, 985, 1204, 1297, 1394, 1469 |
| Hpy166II | GTNNAC | 3 cut(s) | 103, 810, 1530 |
| Hpy188I | TCNGA | 2 cut(s) | 29, 236 |
| Hpy188III | TCNNGA | 8 cut(s) | 190, 362, 757, 1044, 1115, 1433, 1504, 1588 |
| Hpy8I | GTNNAC | 3 cut(s) | 103, 810, 1530 |
| HpyAV | CCTTC | 9 cut(s) | 8, 151, 231, 293, 571, 1084, 1375, 1537, 1574 |
| HpyCH4III | ACNGT | 5 cut(s) | 259, 430, 451, 1339, 1562 |
| HpyCH4IV | ACGT | 4 cut(s) | 265, 1002, 1263, 1354 |
| HpyF10VI | GCNNNNNNNGC | 5 cut(s) | 280, 557, 1136, 1148, 1460 |
| HpyF3I | CTNAG | 4 cut(s) | 153, 800, 1330, 1518 |
| HpySE526I | ACGT | 4 cut(s) | 265, 1002, 1263, 1354 |
| HspAI | GCGC | 3 cut(s) | 478, 918, 1509 |
| Ksp22I | TGATCA | 1 cut(s) | 444 |
| KspAI | GTTAAC | 1 cut(s) | 810 |
| Kzo9I | GATC | 6 cut(s) | 34, 231, 369, 422, 444, 1326 |
| LguI | GCTCTTC | 1 cut(s) | 1045 |
| LmnI | GCTCC | 5 cut(s) | 162, 364, 964, 1093, 1453 |
| Lsp1109I | GCAGC | 7 cut(s) | 126, 185, 286, 560, 563, 1138, 1151 |
| LweI | GCATC | 8 cut(s) | 208, 510, 513, 892, 979, 1384, 1480, 1593 |
| MaeI | CTAG | 2 cut(s) | 1044, 1248 |
| MaeII | ACGT | 4 cut(s) | 265, 1002, 1263, 1354 |
| MaeIII | GTNAC | 8 cut(s) | 266, 328, 642, 904, 1210, 1250, 1333, 1406 |
| MalI | GATC | 6 cut(s) | 36, 233, 371, 424, 446, 1328 |
| MboI | GATC | 6 cut(s) | 34, 231, 369, 422, 444, 1326 |
| MboII | GAAGA | 7 cut(s) | 377, 445, 502, 837, 940, 1032, 1581 |
| MfeI | CAATTG | 1 cut(s) | 858 |
| MflI | RGATCY | 2 cut(s) | 369, 1326 |
| MhlI | GDGCHC | 3 cut(s) | 44, 159, 361 |
| MlsI | TGGCCA | 1 cut(s) | 283 |
| MluCI | AATT | 8 cut(s) | 242, 466, 736, 769, 858, 870, 1047, 1466 |
| MluNI | TGGCCA | 1 cut(s) | 283 |
| Mox20I | TGGCCA | 1 cut(s) | 283 |
| Mph1103I | ATGCAT | 1 cut(s) | 820 |
| MscI | TGGCCA | 1 cut(s) | 283 |
| MseI | TTAA | 5 cut(s) | 180, 465, 534, 809, 1160 |
| MslI | CAYNNNNRTG | 2 cut(s) | 705, 1280 |
| Msp20I | TGGCCA | 1 cut(s) | 283 |
| MspA1I | CMGCKG | 1 cut(s) | 1142 |
| MspI | CCGG | 1 cut(s) | 1445 |
| MspR9I | CCNGG | 1 cut(s) | 161 |
| MunI | CAATTG | 1 cut(s) | 858 |
| Mva1269I | GAATGC | 2 cut(s) | 409, 442 |
| MvaI | CCWGG | 1 cut(s) | 161 |
| MvnI | CGCG | 3 cut(s) | 480, 918, 920 |
| MwoI | GCNNNNNNNGC | 5 cut(s) | 280, 557, 1136, 1148, 1460 |
| NcoI | CCATGG | 1 cut(s) | 378 |
| NdeII | GATC | 6 cut(s) | 34, 231, 369, 422, 444, 1326 |
| NlaIV | GGNNCC | 3 cut(s) | 453, 966, 1449 |
| NmuCI | GTSAC | 6 cut(s) | 266, 328, 642, 904, 1210, 1250 |
| NsiI | ATGCAT | 1 cut(s) | 820 |
| NspI | RCATGY | 3 cut(s) | 23, 822, 1528 |
| OliI | CACNNNNGTG | 1 cut(s) | 705 |
| PagI | TCATGA | 2 cut(s) | 189, 1432 |
| PceI | AGGCCT | 1 cut(s) | 513 |
| PciI | ACATGT | 1 cut(s) | 1524 |
| PciSI | GCTCTTC | 1 cut(s) | 1045 |
| PctI | GAATGC | 2 cut(s) | 409, 442 |
| PfeI | GAWTC | 2 cut(s) | 30, 1288 |
| PkrI | GCNGC | 7 cut(s) | 116, 175, 276, 550, 553, 1141, 1153 |
| PpuMI | RGGWCCY | 1 cut(s) | 302 |
| PscI | ACATGT | 1 cut(s) | 1524 |
| PshBI | ATTAAT | 1 cut(s) | 534 |
| Psp124BI | GAGCTC | 1 cut(s) | 159 |
| Psp1406I | AACGTT | 1 cut(s) | 1354 |
| Psp5II | RGGWCCY | 1 cut(s) | 302 |
| Psp6I | CCWGG | 1 cut(s) | 159 |
| PspGI | CCWGG | 1 cut(s) | 159 |
| PspN4I | GGNNCC | 3 cut(s) | 453, 966, 1449 |
| PspPI | GGNCC | 3 cut(s) | 120, 302, 451 |
| PspPPI | RGGWCCY | 1 cut(s) | 302 |
| PstI | CTGCAG | 3 cut(s) | 511, 550, 1156 |
| PsuI | RGATCY | 2 cut(s) | 369, 1326 |
| PvuII | CAGCTG | 1 cut(s) | 1142 |
| RsaI | GTAC | 2 cut(s) | 606, 1240 |
| RsaNI | GTAC | 2 cut(s) | 605, 1239 |
| RseI | CAYNNNNRTG | 2 cut(s) | 705, 1280 |
| SacI | GAGCTC | 1 cut(s) | 159 |
| SapI | GCTCTTC | 1 cut(s) | 1045 |
| SaqAI | TTAA | 5 cut(s) | 180, 465, 534, 809, 1160 |
| SatI | GCNGC | 7 cut(s) | 115, 174, 275, 549, 552, 1140, 1152 |
| Sau3AI | GATC | 6 cut(s) | 34, 231, 369, 422, 444, 1326 |
| Sau96I | GGNCC | 3 cut(s) | 120, 302, 451 |
| ScaI | AGTACT | 1 cut(s) | 1240 |
| ScrFI | CCNGG | 1 cut(s) | 161 |
| SduI | GDGCHC | 3 cut(s) | 44, 159, 361 |
| SfaNI | GCATC | 8 cut(s) | 208, 510, 513, 892, 979, 1384, 1480, 1593 |
| SfcI | CTRYAG | 4 cut(s) | 507, 546, 1070, 1152 |
| SinI | GGWCC | 3 cut(s) | 120, 302, 451 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 705, 1280 |
| SmlI | CTYRAG | 2 cut(s) | 1082, 1504 |
| SmoI | CTYRAG | 2 cut(s) | 1082, 1504 |
| SpeI | ACTAGT | 1 cut(s) | 1247 |
| Sse9I | AATT | 8 cut(s) | 242, 466, 736, 769, 858, 870, 1047, 1466 |
| SseBI | AGGCCT | 1 cut(s) | 513 |
| SsiI | CCGC | 2 cut(s) | 397, 1454 |
| SspMI | CTAG | 2 cut(s) | 1044, 1248 |
| SstI | GAGCTC | 1 cut(s) | 159 |
| StuI | AGGCCT | 1 cut(s) | 513 |
| StyD4I | CCNGG | 1 cut(s) | 159 |
| StyI | CCWWGG | 2 cut(s) | 378, 703 |
| TaaI | ACNGT | 5 cut(s) | 259, 430, 451, 1339, 1562 |
| TaiI | ACGT | 4 cut(s) | 268, 1005, 1266, 1357 |
| TaqI | TCGA | 2 cut(s) | 33, 720 |
| TasI | AATT | 8 cut(s) | 242, 466, 736, 769, 858, 870, 1047, 1466 |
| TatI | WGTACW | 2 cut(s) | 604, 1238 |
| TfiI | GAWTC | 2 cut(s) | 30, 1288 |
| Tru1I | TTAA | 5 cut(s) | 180, 465, 534, 809, 1160 |
| Tru9I | TTAA | 5 cut(s) | 180, 465, 534, 809, 1160 |
| TscAI | CASTG | 9 cut(s) | 151, 253, 447, 511, 691, 787, 975, 1183, 1567 |
| TseFI | GTSAC | 6 cut(s) | 266, 328, 642, 904, 1210, 1250 |
| TseI | GCWGC | 7 cut(s) | 114, 173, 274, 548, 551, 1139, 1151 |
| Tsp45I | GTSAC | 6 cut(s) | 266, 328, 642, 904, 1210, 1250 |
| TspDTI | ATGAA | 4 cut(s) | 42, 552, 1292, 1421 |
| TspRI | CASTG | 9 cut(s) | 151, 253, 447, 511, 691, 787, 975, 1183, 1567 |
| VpaK11BI | GGWCC | 3 cut(s) | 120, 302, 451 |
| VspI | ATTAAT | 1 cut(s) | 534 |
| XapI | RAATTY | 4 cut(s) | 242, 736, 1047, 1466 |
| XbaI | TCTAGA | 1 cut(s) | 1043 |
| XceI | RCATGY | 3 cut(s) | 23, 822, 1528 |
| XspI | CTAG | 2 cut(s) | 1044, 1248 |
| ZrmI | AGTACT | 1 cut(s) | 1240 |
| Zsp2I | ATGCAT | 1 cut(s) | 820 |