Belongs to the heat shock protein 70 family
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 1731 |
| AatII | GACGTC | 1 cut(s) | 672 |
| AccII | CGCG | 1 cut(s) | 405 |
| AciI | CCGC | 2 cut(s) | 82, 1215 |
| AclWI | GGATC | 5 cut(s) | 50, 436, 885, 953, 966 |
| AcsI | RAATTY | 4 cut(s) | 917, 937, 1041, 1490 |
| AcuI | CTGAAG | 2 cut(s) | 435, 1608 |
| AcyI | GRCGYC | 1 cut(s) | 669 |
| AfaI | GTAC | 4 cut(s) | 625, 966, 1001, 1333 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 1830 |
| AflIII | ACRYGT | 1 cut(s) | 433 |
| AjnI | CCWGG | 1 cut(s) | 189 |
| AjuI | GAANNNNNNNTTGG | 4 cut(s) | 408, 440, 749, 781 |
| AleI | CACNNNNGTG | 1 cut(s) | 699 |
| AloI | GAACNNNNNNTCC | 2 cut(s) | 1122, 1154 |
| Alw21I | GWGCWC | 3 cut(s) | 793, 1500, 1758 |
| Alw26I | GTCTC | 8 cut(s) | 265, 401, 648, 681, 826, 1585, 1810, 1844 |
| AlwI | GGATC | 5 cut(s) | 50, 436, 885, 953, 966 |
| Ama87I | CYCGRG | 1 cut(s) | 786 |
| AoxI | GGCC | 4 cut(s) | 192, 571, 585, 675 |
| ApeKI | GCWGC | 6 cut(s) | 179, 554, 557, 1133, 1145, 1425 |
| ApoI | RAATTY | 4 cut(s) | 917, 937, 1041, 1490 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 647, 679 |
| AseI | ATTAAT | 1 cut(s) | 236 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 941 |
| AspA2I | CCTAGG | 1 cut(s) | 649 |
| AspLEI | GCGC | 1 cut(s) | 405 |
| AsuC2I | CCSGG | 1 cut(s) | 1648 |
| AsuHPI | GGTGA | 8 cut(s) | 185, 686, 716, 739, 1463, 1499, 1721, 1757 |
| AvaI | CYCGRG | 1 cut(s) | 786 |
| AvrII | CCTAGG | 1 cut(s) | 649 |
| BamHI | GGATCC | 1 cut(s) | 958 |
| BanII | GRGCYC | 2 cut(s) | 793, 1500 |
| BauI | CACGAG | 1 cut(s) | 64 |
| BbsI | GAAGAC | 1 cut(s) | 1596 |
| Bbv12I | GWGCWC | 3 cut(s) | 793, 1500, 1758 |
| BbvI | GCAGC | 6 cut(s) | 191, 541, 569, 1132, 1145, 1437 |
| BccI | CCATC | 3 cut(s) | 142, 203, 1388 |
| BceAI | ACGGC | 1 cut(s) | 1582 |
| BciT130I | CCWGG | 1 cut(s) | 191 |
| BciVI | GTATCC | 1 cut(s) | 1212 |
| BclI | TGATCA | 3 cut(s) | 1300, 1312, 1339 |
| BcnI | CCSGG | 1 cut(s) | 1648 |
| BcoDI | GTCTC | 8 cut(s) | 265, 401, 648, 681, 826, 1585, 1810, 1844 |
| BfaI | CTAG | 3 cut(s) | 650, 1038, 1407 |
| BfmI | CTRYAG | 2 cut(s) | 555, 1146 |
| BfuI | GTATCC | 1 cut(s) | 1212 |
| BisI | GCNGC | 6 cut(s) | 180, 555, 558, 1134, 1146, 1426 |
| BlnI | CCTAGG | 1 cut(s) | 649 |
| BlsI | GCNGC | 6 cut(s) | 181, 556, 559, 1135, 1147, 1427 |
| Bme1390I | CCNGG | 2 cut(s) | 191, 1648 |
| BmeT110I | CYCGRG | 1 cut(s) | 786 |
| BmiI | GGNNCC | 1 cut(s) | 960 |
| BmrFI | CCNGG | 2 cut(s) | 191, 1648 |
| BmsI | GCATC | 7 cut(s) | 166, 214, 390, 519, 973, 1183, 1549 |
| BpiI | GAAGAC | 1 cut(s) | 1596 |
| BpmI | CTGGAG | 4 cut(s) | 351, 705, 1497, 1755 |
| Bpu10I | CCTNAGC | 1 cut(s) | 1533 |
| BpuMI | CCSGG | 1 cut(s) | 1648 |
| BsaBI | GATNNNNATC | 2 cut(s) | 1311, 1838 |
| BsaHI | GRCGYC | 1 cut(s) | 669 |
| BsaJI | CCNNGG | 4 cut(s) | 424, 649, 697, 1740 |
| BsaXI | ACNNNNNCTCC | 4 cut(s) | 238, 268, 1122, 1152 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 1830 |
| Bse1I | ACTGG | 2 cut(s) | 171, 1606 |
| Bse8I | GATNNNNATC | 2 cut(s) | 1311, 1838 |
| BseBI | CCWGG | 1 cut(s) | 191 |
| BseDI | CCNNGG | 4 cut(s) | 424, 649, 697, 1740 |
| BseGI | GGATG | 4 cut(s) | 380, 1015, 1075, 1264 |
| BseJI | GATNNNNATC | 2 cut(s) | 1311, 1838 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 1830 |
| BseMII | CTCAG | 3 cut(s) | 1665, 1689, 1839 |
| BseNI | ACTGG | 2 cut(s) | 171, 1606 |
| BseRI | GAGGAG | 1 cut(s) | 844 |
| BseXI | GCAGC | 6 cut(s) | 191, 541, 569, 1132, 1145, 1437 |
| Bsh1236I | CGCG | 1 cut(s) | 405 |
| BshFI | GGCC | 4 cut(s) | 194, 573, 587, 677 |
| BsiHKAI | GWGCWC | 3 cut(s) | 793, 1500, 1758 |
| BsiHKCI | CYCGRG | 1 cut(s) | 786 |
| BsiSI | CCGG | 1 cut(s) | 1647 |
| BslFI | GGGAC | 1 cut(s) | 443 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 1830 |
| BsmAI | GTCTC | 8 cut(s) | 265, 401, 648, 681, 826, 1585, 1810, 1844 |
| BsmBI | CGTCTC | 1 cut(s) | 648 |
| BsmFI | GGGAC | 1 cut(s) | 443 |
| BsmI | GAATGC | 3 cut(s) | 448, 505, 1569 |
| BsnI | GGCC | 4 cut(s) | 194, 573, 587, 677 |
| BsoBI | CYCGRG | 1 cut(s) | 786 |
| Bsp1286I | GDGCHC | 3 cut(s) | 793, 1500, 1758 |
| Bsp1407I | TGTACA | 2 cut(s) | 964, 999 |
| Bsp143I | GATC | 8 cut(s) | 42, 112, 428, 877, 958, 1300, 1312, 1339 |
| Bsp19I | CCATGG | 1 cut(s) | 1740 |
| BspACI | CCGC | 2 cut(s) | 82, 1215 |
| BspANI | GGCC | 4 cut(s) | 194, 573, 587, 677 |
| BspCNI | CTCAG | 3 cut(s) | 1666, 1690, 1840 |
| BspFNI | CGCG | 1 cut(s) | 405 |
| BspHI | TCATGA | 2 cut(s) | 1303, 1336 |
| BspLI | GGNNCC | 1 cut(s) | 960 |
| BspMAI | CTGCAG | 2 cut(s) | 559, 1150 |
| BspPI | GGATC | 5 cut(s) | 50, 436, 885, 953, 966 |
| BspQI | GCTCTTC | 1 cut(s) | 1039 |
| BsrGI | TGTACA | 2 cut(s) | 964, 999 |
| BsrI | ACTGG | 2 cut(s) | 171, 1606 |
| BssECI | CCNNGG | 4 cut(s) | 424, 649, 697, 1740 |
| BssMI | GATC | 8 cut(s) | 42, 112, 428, 877, 958, 1300, 1312, 1339 |
| BssNI | GRCGYC | 1 cut(s) | 669 |
| BssSI | CACGAG | 1 cut(s) | 64 |
| BssT1I | CCWWGG | 4 cut(s) | 424, 649, 697, 1740 |
| Bst2BI | CACGAG | 1 cut(s) | 64 |
| Bst2UI | CCWGG | 1 cut(s) | 191 |
| Bst4CI | ACNGT | 4 cut(s) | 457, 659, 872, 1004 |
| Bst6I | CTCTTC | 2 cut(s) | 18, 1039 |
| BstACI | GRCGYC | 1 cut(s) | 669 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 563 |
| BstAUI | TGTACA | 2 cut(s) | 964, 999 |
| BstC8I | GCNNGC | 1 cut(s) | 988 |
| BstDEI | CTNAG | 5 cut(s) | 1533, 1632, 1674, 1698, 1848 |
| BstDSI | CCRYGG | 1 cut(s) | 1740 |
| BstF5I | GGATG | 4 cut(s) | 380, 1015, 1075, 1264 |
| BstFNI | CGCG | 1 cut(s) | 405 |
| BstHHI | GCGC | 1 cut(s) | 405 |
| BstKTI | GATC | 8 cut(s) | 45, 115, 431, 880, 961, 1303, 1315, 1342 |
| BstMAI | GTCTC | 8 cut(s) | 265, 401, 648, 681, 826, 1585, 1810, 1844 |
| BstMBI | GATC | 8 cut(s) | 42, 112, 428, 877, 958, 1300, 1312, 1339 |
| BstMWI | GCNNNNNNNGC | 4 cut(s) | 563, 987, 1130, 1142 |
| BstNI | CCWGG | 1 cut(s) | 191 |
| BstNSI | RCATGY | 2 cut(s) | 437, 1787 |
| BstSCI | CCNGG | 2 cut(s) | 189, 1646 |
| BstSFI | CTRYAG | 2 cut(s) | 555, 1146 |
| BstUI | CGCG | 1 cut(s) | 405 |
| BstV1I | GCAGC | 6 cut(s) | 191, 541, 569, 1132, 1145, 1437 |
| BstV2I | GAAGAC | 1 cut(s) | 1596 |
| BstX2I | RGATCY | 1 cut(s) | 958 |
| BstYI | RGATCY | 1 cut(s) | 958 |
| BsuI | GTATCC | 1 cut(s) | 1212 |
| BsuRI | GGCC | 4 cut(s) | 194, 573, 587, 677 |
| BtgI | CCRYGG | 1 cut(s) | 1740 |
| BtgZI | GCGATG | 1 cut(s) | 996 |
| BtsCI | GGATG | 4 cut(s) | 380, 1015, 1075, 1264 |
| BtsIMutI | CAGTG | 5 cut(s) | 150, 453, 1009, 1599, 1845 |
| Cac8I | GCNNGC | 1 cut(s) | 988 |
| CciI | TCATGA | 2 cut(s) | 1303, 1336 |
| CfoI | GCGC | 1 cut(s) | 405 |
| Csp6I | GTAC | 4 cut(s) | 624, 965, 1000, 1332 |
| CviAII | CATG | 8 cut(s) | 385, 434, 991, 1304, 1337, 1741, 1784, 1861 |
| CviQI | GTAC | 4 cut(s) | 624, 965, 1000, 1332 |
| DdeI | CTNAG | 5 cut(s) | 1533, 1632, 1674, 1698, 1848 |
| DpnI | GATC | 8 cut(s) | 44, 114, 430, 879, 960, 1302, 1314, 1341 |
| DpnII | GATC | 8 cut(s) | 42, 112, 428, 877, 958, 1300, 1312, 1339 |
| Eam1104I | CTCTTC | 2 cut(s) | 18, 1039 |
| EarI | CTCTTC | 2 cut(s) | 18, 1039 |
| EciI | GGCGGA | 1 cut(s) | 97 |
| Ecl136II | GAGCTC | 2 cut(s) | 791, 1498 |
| Eco130I | CCWWGG | 4 cut(s) | 424, 649, 697, 1740 |
| Eco24I | GRGCYC | 2 cut(s) | 793, 1500 |
| Eco53kI | GAGCTC | 2 cut(s) | 791, 1498 |
| Eco57I | CTGAAG | 2 cut(s) | 435, 1608 |
| Eco88I | CYCGRG | 1 cut(s) | 786 |
| EcoICRI | GAGCTC | 2 cut(s) | 791, 1498 |
| EcoRI | GAATTC | 1 cut(s) | 1041 |
| EcoRII | CCWGG | 1 cut(s) | 189 |
| EcoT14I | CCWWGG | 4 cut(s) | 424, 649, 697, 1740 |
| EcoT38I | GRGCYC | 2 cut(s) | 793, 1500 |
| ErhI | CCWWGG | 4 cut(s) | 424, 649, 697, 1740 |
| Esp3I | CGTCTC | 1 cut(s) | 648 |
| FaeI | CATG | 8 cut(s) | 388, 437, 994, 1307, 1340, 1744, 1787, 1864 |
| FalI | AAGNNNNNCTT | 4 cut(s) | 408, 440, 796, 828 |
| FaqI | GGGAC | 1 cut(s) | 443 |
| FatI | CATG | 8 cut(s) | 384, 433, 990, 1303, 1336, 1740, 1783, 1860 |
| FauI | CCCGC | 1 cut(s) | 1208 |
| FauNDI | CATATG | 1 cut(s) | 1126 |
| FbaI | TGATCA | 3 cut(s) | 1300, 1312, 1339 |
| Fnu4HI | GCNGC | 6 cut(s) | 180, 555, 558, 1134, 1146, 1426 |
| FokI | GGATG | 4 cut(s) | 367, 1022, 1082, 1271 |
| FriOI | GRGCYC | 2 cut(s) | 793, 1500 |
| Fsp4HI | GCNGC | 6 cut(s) | 180, 555, 558, 1134, 1146, 1426 |
| FspBI | CTAG | 3 cut(s) | 650, 1038, 1407 |
| GlaI | GCGC | 1 cut(s) | 404 |
| GluI | GCNGC | 6 cut(s) | 180, 555, 558, 1134, 1146, 1426 |
| GsuI | CTGGAG | 4 cut(s) | 351, 705, 1497, 1755 |
| HaeIII | GGCC | 4 cut(s) | 194, 573, 587, 677 |
| HapII | CCGG | 1 cut(s) | 1647 |
| HhaI | GCGC | 1 cut(s) | 405 |
| Hin1I | GRCGYC | 1 cut(s) | 669 |
| Hin1II | CATG | 8 cut(s) | 388, 437, 994, 1307, 1340, 1744, 1787, 1864 |
| Hin6I | GCGC | 1 cut(s) | 403 |
| HinP1I | GCGC | 1 cut(s) | 403 |
| HindIII | AAGCTT | 1 cut(s) | 1535 |
| HinfI | GANTC | 4 cut(s) | 328, 388, 1357, 1820 |
| HpaII | CCGG | 1 cut(s) | 1647 |
| HphI | GGTGA | 8 cut(s) | 185, 686, 716, 739, 1463, 1499, 1721, 1757 |
| Hpy166II | GTNNAC | 3 cut(s) | 1000, 1599, 1824 |
| Hpy188I | TCNGA | 4 cut(s) | 200, 333, 1047, 1344 |
| Hpy8I | GTNNAC | 3 cut(s) | 1000, 1599, 1824 |
| HpyAV | CCTTC | 7 cut(s) | 157, 299, 577, 1042, 1078, 1442, 1700 |
| HpyCH4III | ACNGT | 4 cut(s) | 457, 659, 872, 1004 |
| HpyCH4IV | ACGT | 6 cut(s) | 141, 484, 525, 669, 771, 933 |
| HpyF10VI | GCNNNNNNNGC | 4 cut(s) | 563, 987, 1130, 1142 |
| HpyF3I | CTNAG | 5 cut(s) | 1533, 1632, 1674, 1698, 1848 |
| HpySE526I | ACGT | 6 cut(s) | 141, 484, 525, 669, 771, 933 |
| Hsp92I | GRCGYC | 1 cut(s) | 669 |
| Hsp92II | CATG | 8 cut(s) | 388, 437, 994, 1307, 1340, 1744, 1787, 1864 |
| HspAI | GCGC | 1 cut(s) | 403 |
| Ksp22I | TGATCA | 3 cut(s) | 1300, 1312, 1339 |
| Kzo9I | GATC | 8 cut(s) | 42, 112, 428, 877, 958, 1300, 1312, 1339 |
| LguI | GCTCTTC | 1 cut(s) | 1039 |
| LmnI | GCTCC | 4 cut(s) | 370, 1130, 1522, 1780 |
| Lsp1109I | GCAGC | 6 cut(s) | 191, 541, 569, 1132, 1145, 1437 |
| LweI | GCATC | 7 cut(s) | 166, 214, 390, 519, 973, 1183, 1549 |
| MaeI | CTAG | 3 cut(s) | 650, 1038, 1407 |
| MaeII | ACGT | 6 cut(s) | 141, 484, 525, 669, 771, 933 |
| MaeIII | GTNAC | 3 cut(s) | 636, 1261, 1693 |
| MalI | GATC | 8 cut(s) | 44, 114, 430, 879, 960, 1302, 1314, 1341 |
| MboI | GATC | 8 cut(s) | 42, 112, 428, 877, 958, 1300, 1312, 1339 |
| MboII | GAAGA | 9 cut(s) | 29, 32, 35, 383, 508, 719, 840, 1026, 1601 |
| MfeI | CAATTG | 2 cut(s) | 561, 852 |
| MflI | RGATCY | 1 cut(s) | 958 |
| MhlI | GDGCHC | 3 cut(s) | 793, 1500, 1758 |
| MlyI | GAGTC | 1 cut(s) | 1829 |
| MroXI | GAANNNNTTC | 1 cut(s) | 941 |
| MseI | TTAA | 9 cut(s) | 186, 236, 279, 521, 540, 803, 1154, 1202, 1575 |
| MslI | CAYNNNNRTG | 5 cut(s) | 699, 995, 1308, 1320, 1385 |
| MspA1I | CMGCKG | 3 cut(s) | 554, 683, 1136 |
| MspI | CCGG | 1 cut(s) | 1647 |
| MspR9I | CCNGG | 2 cut(s) | 191, 1648 |
| MunI | CAATTG | 2 cut(s) | 561, 852 |
| Mva1269I | GAATGC | 3 cut(s) | 448, 505, 1569 |
| MvaI | CCWGG | 1 cut(s) | 191 |
| MvnI | CGCG | 1 cut(s) | 405 |
| MwoI | GCNNNNNNNGC | 4 cut(s) | 563, 987, 1130, 1142 |
| NciI | CCSGG | 1 cut(s) | 1648 |
| NcoI | CCATGG | 1 cut(s) | 1740 |
| NdeI | CATATG | 1 cut(s) | 1126 |
| NdeII | GATC | 8 cut(s) | 42, 112, 428, 877, 958, 1300, 1312, 1339 |
| NlaIII | CATG | 8 cut(s) | 388, 437, 994, 1307, 1340, 1744, 1787, 1864 |
| NlaIV | GGNNCC | 1 cut(s) | 960 |
| NmuCI | GTSAC | 3 cut(s) | 636, 1261, 1693 |
| NspI | RCATGY | 2 cut(s) | 437, 1787 |
| OliI | CACNNNNGTG | 1 cut(s) | 699 |
| PagI | TCATGA | 2 cut(s) | 1303, 1336 |
| PciI | ACATGT | 1 cut(s) | 433 |
| PciSI | GCTCTTC | 1 cut(s) | 1039 |
| PctI | GAATGC | 3 cut(s) | 448, 505, 1569 |
| PdmI | GAANNNNTTC | 1 cut(s) | 941 |
| PfeI | GAWTC | 3 cut(s) | 328, 388, 1357 |
| PkrI | GCNGC | 6 cut(s) | 181, 556, 559, 1135, 1147, 1427 |
| PleI | GAGTC | 1 cut(s) | 1828 |
| PpsI | GAGTC | 1 cut(s) | 1828 |
| PscI | ACATGT | 1 cut(s) | 433 |
| PshBI | ATTAAT | 1 cut(s) | 236 |
| PsiI | TTATAA | 1 cut(s) | 1731 |
| Psp124BI | GAGCTC | 2 cut(s) | 793, 1500 |
| Psp6I | CCWGG | 1 cut(s) | 189 |
| PspGI | CCWGG | 1 cut(s) | 189 |
| PspN4I | GGNNCC | 1 cut(s) | 960 |
| PstI | CTGCAG | 2 cut(s) | 559, 1150 |
| PsuI | RGATCY | 1 cut(s) | 958 |
| PvuII | CAGCTG | 3 cut(s) | 554, 683, 1136 |
| RsaI | GTAC | 4 cut(s) | 625, 966, 1001, 1333 |
| RsaNI | GTAC | 4 cut(s) | 624, 965, 1000, 1332 |
| RseI | CAYNNNNRTG | 5 cut(s) | 699, 995, 1308, 1320, 1385 |
| SacI | GAGCTC | 2 cut(s) | 793, 1500 |
| SapI | GCTCTTC | 1 cut(s) | 1039 |
| SaqAI | TTAA | 9 cut(s) | 186, 236, 279, 521, 540, 803, 1154, 1202, 1575 |
| SatI | GCNGC | 6 cut(s) | 180, 555, 558, 1134, 1146, 1426 |
| Sau3AI | GATC | 8 cut(s) | 42, 112, 428, 877, 958, 1300, 1312, 1339 |
| SchI | GAGTC | 1 cut(s) | 1829 |
| ScrFI | CCNGG | 2 cut(s) | 191, 1648 |
| SduI | GDGCHC | 3 cut(s) | 793, 1500, 1758 |
| SfaNI | GCATC | 7 cut(s) | 166, 214, 390, 519, 973, 1183, 1549 |
| SfcI | CTRYAG | 2 cut(s) | 555, 1146 |
| SmiMI | CAYNNNNRTG | 5 cut(s) | 699, 995, 1308, 1320, 1385 |
| SsiI | CCGC | 2 cut(s) | 82, 1215 |
| SspI | AATATT | 1 cut(s) | 1765 |
| SspMI | CTAG | 3 cut(s) | 650, 1038, 1407 |
| SstI | GAGCTC | 2 cut(s) | 793, 1500 |
| StyD4I | CCNGG | 2 cut(s) | 189, 1646 |
| StyI | CCWWGG | 4 cut(s) | 424, 649, 697, 1740 |
| TaaI | ACNGT | 4 cut(s) | 457, 659, 872, 1004 |
| TaiI | ACGT | 6 cut(s) | 144, 487, 528, 672, 774, 936 |
| TaqI | TCGA | 2 cut(s) | 45, 303 |
| TatI | WGTACW | 3 cut(s) | 964, 999, 1331 |
| TfiI | GAWTC | 3 cut(s) | 328, 388, 1357 |
| Tru1I | TTAA | 9 cut(s) | 186, 236, 279, 521, 540, 803, 1154, 1202, 1575 |
| Tru9I | TTAA | 9 cut(s) | 186, 236, 279, 521, 540, 803, 1154, 1202, 1575 |
| TscAI | CASTG | 5 cut(s) | 157, 460, 1009, 1606, 1852 |
| TseFI | GTSAC | 3 cut(s) | 636, 1261, 1693 |
| TseI | GCWGC | 6 cut(s) | 179, 554, 557, 1133, 1145, 1425 |
| Tsp45I | GTSAC | 3 cut(s) | 636, 1261, 1693 |
| TspDTI | ATGAA | 4 cut(s) | 828, 1403, 1461, 1719 |
| TspRI | CASTG | 5 cut(s) | 157, 460, 1009, 1606, 1852 |
| VspI | ATTAAT | 1 cut(s) | 236 |
| XapI | RAATTY | 4 cut(s) | 917, 937, 1041, 1490 |
| XbaI | TCTAGA | 1 cut(s) | 1037 |
| XceI | RCATGY | 2 cut(s) | 437, 1787 |
| XmaJI | CCTAGG | 1 cut(s) | 649 |
| XmnI | GAANNNNTTC | 1 cut(s) | 941 |
| XspI | CTAG | 3 cut(s) | 650, 1038, 1407 |
| ZraI | GACGTC | 1 cut(s) | 670 |