Heat shock cognate 70 kDa
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AarI | CACCTGC | 1 cut(s) | 970 |
| Acc36I | ACCTGC | 1 cut(s) | 970 |
| AccII | CGCG | 1 cut(s) | 1056 |
| AciI | CCGC | 5 cut(s) | 79, 824, 1054, 1351, 1566 |
| AclWI | GGATC | 1 cut(s) | 937 |
| AcsI | RAATTY | 1 cut(s) | 1076 |
| AcuI | CTGAAG | 1 cut(s) | 66 |
| AfaI | GTAC | 3 cut(s) | 1116, 1260, 1535 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 1592, 1595 |
| AjnI | CCWGG | 2 cut(s) | 1009, 1536 |
| Alw21I | GWGCWC | 3 cut(s) | 481, 1086, 1299 |
| Alw26I | GTCTC | 4 cut(s) | 254, 328, 908, 1171 |
| AlwI | GGATC | 1 cut(s) | 937 |
| AoxI | GGCC | 1 cut(s) | 93 |
| ApeKI | GCWGC | 9 cut(s) | 41, 157, 230, 233, 751, 821, 833, 1394, 1547 |
| ApoI | RAATTY | 1 cut(s) | 1076 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 1581 |
| AspS9I | GGNCC | 2 cut(s) | 871, 1610 |
| AsuC2I | CCSGG | 1 cut(s) | 797 |
| AsuHPI | GGTGA | 8 cut(s) | 35, 124, 386, 427, 586, 860, 1049, 1501 |
| AvaII | GGWCC | 2 cut(s) | 871, 1610 |
| BanII | GRGCYC | 3 cut(s) | 225, 481, 1086 |
| BbsI | GAAGAC | 2 cut(s) | 1234, 1584 |
| Bbv12I | GWGCWC | 3 cut(s) | 481, 1086, 1299 |
| BbvCI | CCTCAGC | 1 cut(s) | 1173 |
| BbvI | GCAGC | 9 cut(s) | 28, 169, 217, 245, 738, 808, 820, 1406, 1534 |
| BccI | CCATC | 5 cut(s) | 406, 673, 1217, 1249, 1442 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 1127, 1161 |
| BciT130I | CCWGG | 2 cut(s) | 1011, 1538 |
| BcnI | CCSGG | 1 cut(s) | 797 |
| BcoDI | GTCTC | 4 cut(s) | 254, 328, 908, 1171 |
| BfaI | CTAG | 2 cut(s) | 726, 884 |
| BfmI | CTRYAG | 1 cut(s) | 231 |
| BfuAI | ACCTGC | 1 cut(s) | 970 |
| BglII | AGATCT | 1 cut(s) | 51 |
| Bme1390I | CCNGG | 3 cut(s) | 797, 1011, 1538 |
| Bme18I | GGWCC | 2 cut(s) | 871, 1610 |
| BmgT120I | GGNCC | 2 cut(s) | 871, 1610 |
| BmiI | GGNNCC | 2 cut(s) | 648, 1612 |
| BmrFI | CCNGG | 3 cut(s) | 797, 1011, 1538 |
| BmsI | GCATC | 9 cut(s) | 66, 166, 195, 661, 798, 1135, 1164, 1381, 1408 |
| BpiI | GAAGAC | 2 cut(s) | 1234, 1584 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 542, 574 |
| BpmI | CTGGAG | 4 cut(s) | 201, 942, 1083, 1349 |
| Bpu10I | CCTNAGC | 1 cut(s) | 1173 |
| BpuMI | CCSGG | 1 cut(s) | 797 |
| BsaBI | GATNNNNATC | 1 cut(s) | 1208 |
| BsaI | GGTCTC | 1 cut(s) | 254 |
| BsaJI | CCNNGG | 5 cut(s) | 903, 947, 1106, 1236, 1537 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 968, 998 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 1592, 1595 |
| Bse1I | ACTGG | 3 cut(s) | 267, 650, 1192 |
| Bse3DI | GCAATG | 3 cut(s) | 186, 862, 1521 |
| Bse8I | GATNNNNATC | 1 cut(s) | 1208 |
| BseBI | CCWGG | 2 cut(s) | 1011, 1538 |
| BseDI | CCNNGG | 5 cut(s) | 903, 947, 1106, 1236, 1537 |
| BseGI | GGATG | 7 cut(s) | 181, 417, 527, 805, 1155, 1423, 1579 |
| BseJI | GATNNNNATC | 1 cut(s) | 1208 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 1592, 1595 |
| BseMI | GCAATG | 3 cut(s) | 186, 862, 1521 |
| BseMII | CTCAG | 3 cut(s) | 551, 1164, 1260 |
| BseNI | ACTGG | 3 cut(s) | 267, 650, 1192 |
| BseRI | GAGGAG | 4 cut(s) | 405, 885, 1307, 1487 |
| BseXI | GCAGC | 9 cut(s) | 28, 169, 217, 245, 738, 808, 820, 1406, 1534 |
| BsgI | GTGCAG | 1 cut(s) | 887 |
| Bsh1236I | CGCG | 1 cut(s) | 1056 |
| BshFI | GGCC | 1 cut(s) | 95 |
| BsiHKAI | GWGCWC | 3 cut(s) | 481, 1086, 1299 |
| BsiSI | CCGG | 1 cut(s) | 797 |
| BslFI | GGGAC | 1 cut(s) | 1596 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 1592, 1595 |
| BsmAI | GTCTC | 4 cut(s) | 254, 328, 908, 1171 |
| BsmFI | GGGAC | 1 cut(s) | 1596 |
| BsmI | GAATGC | 2 cut(s) | 1092, 1327 |
| BsnI | GGCC | 1 cut(s) | 95 |
| Bso31I | GGTCTC | 1 cut(s) | 254 |
| Bsp1286I | GDGCHC | 4 cut(s) | 225, 481, 1086, 1299 |
| Bsp143I | GATC | 4 cut(s) | 51, 298, 942, 1518 |
| BspACI | CCGC | 5 cut(s) | 79, 824, 1054, 1351, 1566 |
| BspANI | GGCC | 1 cut(s) | 95 |
| BspCNI | CTCAG | 3 cut(s) | 550, 1165, 1261 |
| BspFNI | CGCG | 1 cut(s) | 1056 |
| BspLI | GGNNCC | 2 cut(s) | 648, 1612 |
| BspMAI | CTGCAG | 1 cut(s) | 235 |
| BspMI | ACCTGC | 1 cut(s) | 970 |
| BspPI | GGATC | 1 cut(s) | 937 |
| BspQI | GCTCTTC | 1 cut(s) | 606 |
| BspTNI | GGTCTC | 1 cut(s) | 254 |
| BsrDI | GCAATG | 3 cut(s) | 186, 862, 1521 |
| BsrI | ACTGG | 3 cut(s) | 267, 650, 1192 |
| BssECI | CCNNGG | 5 cut(s) | 903, 947, 1106, 1236, 1537 |
| BssMI | GATC | 4 cut(s) | 51, 298, 942, 1518 |
| BssT1I | CCWWGG | 4 cut(s) | 903, 947, 1106, 1236 |
| Bst2UI | CCWGG | 2 cut(s) | 1011, 1538 |
| Bst4CI | ACNGT | 5 cut(s) | 112, 133, 937, 961, 1129 |
| Bst6I | CTCTTC | 5 cut(s) | 21, 42, 336, 509, 606 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 748 |
| BstC8I | GCNNGC | 3 cut(s) | 138, 831, 1564 |
| BstDEI | CTNAG | 4 cut(s) | 482, 537, 1173, 1269 |
| BstF5I | GGATG | 7 cut(s) | 181, 417, 527, 805, 1155, 1423, 1579 |
| BstFNI | CGCG | 1 cut(s) | 1056 |
| BstKTI | GATC | 4 cut(s) | 54, 301, 945, 1521 |
| BstMAI | GTCTC | 4 cut(s) | 254, 328, 908, 1171 |
| BstMBI | GATC | 4 cut(s) | 51, 298, 942, 1518 |
| BstMWI | GCNNNNNNNGC | 4 cut(s) | 239, 748, 818, 830 |
| BstNI | CCWGG | 2 cut(s) | 1011, 1538 |
| BstNSI | RCATGY | 1 cut(s) | 1351 |
| BstSCI | CCNGG | 3 cut(s) | 795, 1009, 1536 |
| BstSFI | CTRYAG | 1 cut(s) | 231 |
| BstUI | CGCG | 1 cut(s) | 1056 |
| BstV1I | GCAGC | 9 cut(s) | 28, 169, 217, 245, 738, 808, 820, 1406, 1534 |
| BstV2I | GAAGAC | 2 cut(s) | 1234, 1584 |
| BstX2I | RGATCY | 1 cut(s) | 51 |
| BstYI | RGATCY | 1 cut(s) | 51 |
| BsuRI | GGCC | 1 cut(s) | 95 |
| BtsCI | GGATG | 7 cut(s) | 181, 417, 527, 805, 1155, 1423, 1579 |
| BtsI | GCAGTG | 4 cut(s) | 225, 531, 697, 1606 |
| BtsIMutI | CAGTG | 6 cut(s) | 225, 274, 531, 697, 1185, 1606 |
| BveI | ACCTGC | 1 cut(s) | 970 |
| Cac8I | GCNNGC | 3 cut(s) | 138, 831, 1564 |
| Cfr13I | GGNCC | 2 cut(s) | 871, 1610 |
| CseI | GACGC | 1 cut(s) | 149 |
| CsiI | ACCWGGT | 1 cut(s) | 1009 |
| Csp6I | GTAC | 3 cut(s) | 1115, 1259, 1534 |
| CviAII | CATG | 6 cut(s) | 622, 643, 698, 1348, 1558, 1570 |
| CviQI | GTAC | 3 cut(s) | 1115, 1259, 1534 |
| DdeI | CTNAG | 4 cut(s) | 482, 537, 1173, 1269 |
| DpnI | GATC | 4 cut(s) | 53, 300, 944, 1520 |
| DpnII | GATC | 4 cut(s) | 51, 298, 942, 1518 |
| Eam1104I | CTCTTC | 5 cut(s) | 21, 42, 336, 509, 606 |
| EarI | CTCTTC | 5 cut(s) | 21, 42, 336, 509, 606 |
| Ecl136II | GAGCTC | 2 cut(s) | 479, 1084 |
| Eco130I | CCWWGG | 4 cut(s) | 903, 947, 1106, 1236 |
| Eco147I | AGGCCT | 1 cut(s) | 95 |
| Eco24I | GRGCYC | 3 cut(s) | 225, 481, 1086 |
| Eco31I | GGTCTC | 1 cut(s) | 254 |
| Eco32I | GATATC | 1 cut(s) | 1246 |
| Eco47I | GGWCC | 2 cut(s) | 871, 1610 |
| Eco53kI | GAGCTC | 2 cut(s) | 479, 1084 |
| Eco57I | CTGAAG | 1 cut(s) | 66 |
| EcoICRI | GAGCTC | 2 cut(s) | 479, 1084 |
| EcoO109I | RGGNCCY | 1 cut(s) | 871 |
| EcoRII | CCWGG | 2 cut(s) | 1009, 1536 |
| EcoRV | GATATC | 1 cut(s) | 1246 |
| EcoT14I | CCWWGG | 4 cut(s) | 903, 947, 1106, 1236 |
| EcoT38I | GRGCYC | 3 cut(s) | 225, 481, 1086 |
| ErhI | CCWWGG | 4 cut(s) | 903, 947, 1106, 1236 |
| FaeI | CATG | 6 cut(s) | 625, 646, 701, 1351, 1561, 1573 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 272, 304 |
| FaqI | GGGAC | 1 cut(s) | 1596 |
| FatI | CATG | 6 cut(s) | 621, 642, 697, 1347, 1557, 1569 |
| FauI | CCCGC | 2 cut(s) | 72, 1061 |
| FokI | GGATG | 7 cut(s) | 188, 424, 514, 812, 1142, 1430, 1586 |
| FriOI | GRGCYC | 3 cut(s) | 225, 481, 1086 |
| FspBI | CTAG | 2 cut(s) | 726, 884 |
| GsuI | CTGGAG | 4 cut(s) | 201, 942, 1083, 1349 |
| HaeIII | GGCC | 1 cut(s) | 95 |
| HapII | CCGG | 1 cut(s) | 797 |
| HgaI | GACGC | 1 cut(s) | 149 |
| Hin1II | CATG | 6 cut(s) | 625, 646, 701, 1351, 1561, 1573 |
| HindIII | AAGCTT | 1 cut(s) | 1385 |
| HinfI | GANTC | 8 cut(s) | 152, 183, 418, 520, 557, 730, 1021, 1313 |
| HpaII | CCGG | 1 cut(s) | 797 |
| HphI | GGTGA | 8 cut(s) | 35, 124, 386, 427, 586, 860, 1049, 1501 |
| Hpy166II | GTNNAC | 1 cut(s) | 1115 |
| Hpy188I | TCNGA | 4 cut(s) | 540, 966, 1000, 1285 |
| Hpy188III | TCNNGA | 9 cut(s) | 73, 251, 289, 431, 439, 1018, 1249, 1328, 1553 |
| Hpy8I | GTNNAC | 1 cut(s) | 1115 |
| HpyAV | CCTTC | 7 cut(s) | 166, 253, 448, 856, 967, 1300, 1486 |
| HpyCH4III | ACNGT | 5 cut(s) | 112, 133, 937, 961, 1129 |
| HpyCH4IV | ACGT | 1 cut(s) | 1581 |
| HpyF10VI | GCNNNNNNNGC | 4 cut(s) | 239, 748, 818, 830 |
| HpyF3I | CTNAG | 4 cut(s) | 482, 537, 1173, 1269 |
| HpySE526I | ACGT | 1 cut(s) | 1581 |
| Hsp92II | CATG | 6 cut(s) | 625, 646, 701, 1351, 1561, 1573 |
| Kzo9I | GATC | 4 cut(s) | 51, 298, 942, 1518 |
| LguI | GCTCTTC | 1 cut(s) | 606 |
| LmnI | GCTCC | 7 cut(s) | 646, 885, 976, 1108, 1294, 1495, 1555 |
| Lsp1109I | GCAGC | 9 cut(s) | 28, 169, 217, 245, 738, 808, 820, 1406, 1534 |
| LweI | GCATC | 9 cut(s) | 66, 166, 195, 661, 798, 1135, 1164, 1381, 1408 |
| MabI | ACCWGGT | 1 cut(s) | 1009 |
| MaeI | CTAG | 2 cut(s) | 726, 884 |
| MaeII | ACGT | 1 cut(s) | 1581 |
| MaeIII | GTNAC | 2 cut(s) | 127, 889 |
| MalI | GATC | 4 cut(s) | 53, 300, 944, 1520 |
| MboI | GATC | 4 cut(s) | 51, 298, 942, 1518 |
| MfeI | CAATTG | 3 cut(s) | 336, 746, 1422 |
| MflI | RGATCY | 1 cut(s) | 51 |
| MhlI | GDGCHC | 4 cut(s) | 225, 481, 1086, 1299 |
| MluCI | AATT | 7 cut(s) | 35, 336, 746, 1076, 1333, 1422, 1434 |
| MlyI | GAGTC | 5 cut(s) | 146, 192, 514, 551, 1322 |
| MmeI | TCCRAC | 3 cut(s) | 253, 989, 1263 |
| MroXI | GAANNNNTTC | 1 cut(s) | 1581 |
| MseI | TTAA | 3 cut(s) | 198, 1362, 1633 |
| MslI | CAYNNNNRTG | 1 cut(s) | 74 |
| MspI | CCGG | 1 cut(s) | 797 |
| MspR9I | CCNGG | 3 cut(s) | 797, 1011, 1538 |
| MunI | CAATTG | 3 cut(s) | 336, 746, 1422 |
| Mva1269I | GAATGC | 2 cut(s) | 1092, 1327 |
| MvaI | CCWGG | 2 cut(s) | 1011, 1538 |
| MvnI | CGCG | 1 cut(s) | 1056 |
| MwoI | GCNNNNNNNGC | 4 cut(s) | 239, 748, 818, 830 |
| NciI | CCSGG | 1 cut(s) | 797 |
| NdeII | GATC | 4 cut(s) | 51, 298, 942, 1518 |
| NlaIII | CATG | 6 cut(s) | 625, 646, 701, 1351, 1561, 1573 |
| NlaIV | GGNNCC | 2 cut(s) | 648, 1612 |
| NmeAIII | GCCGAG | 1 cut(s) | 81 |
| NmuCI | GTSAC | 1 cut(s) | 889 |
| NspI | RCATGY | 1 cut(s) | 1351 |
| PaqCI | CACCTGC | 1 cut(s) | 970 |
| PceI | AGGCCT | 1 cut(s) | 95 |
| PciSI | GCTCTTC | 1 cut(s) | 606 |
| PctI | GAATGC | 2 cut(s) | 1092, 1327 |
| PdmI | GAANNNNTTC | 1 cut(s) | 1581 |
| PfeI | GAWTC | 3 cut(s) | 418, 730, 1021 |
| PleI | GAGTC | 5 cut(s) | 146, 191, 514, 551, 1321 |
| PpsI | GAGTC | 5 cut(s) | 146, 191, 514, 551, 1321 |
| PpuMI | RGGWCCY | 1 cut(s) | 871 |
| Psp124BI | GAGCTC | 2 cut(s) | 481, 1086 |
| Psp5II | RGGWCCY | 1 cut(s) | 871 |
| Psp6I | CCWGG | 2 cut(s) | 1009, 1536 |
| PspGI | CCWGG | 2 cut(s) | 1009, 1536 |
| PspN4I | GGNNCC | 2 cut(s) | 648, 1612 |
| PspPI | GGNCC | 2 cut(s) | 871, 1610 |
| PspPPI | RGGWCCY | 1 cut(s) | 871 |
| PstI | CTGCAG | 1 cut(s) | 235 |
| PsuI | RGATCY | 1 cut(s) | 51 |
| RsaI | GTAC | 3 cut(s) | 1116, 1260, 1535 |
| RsaNI | GTAC | 3 cut(s) | 1115, 1259, 1534 |
| RseI | CAYNNNNRTG | 1 cut(s) | 74 |
| SacI | GAGCTC | 2 cut(s) | 481, 1086 |
| SapI | GCTCTTC | 1 cut(s) | 606 |
| SaqAI | TTAA | 3 cut(s) | 198, 1362, 1633 |
| Sau3AI | GATC | 4 cut(s) | 51, 298, 942, 1518 |
| Sau96I | GGNCC | 2 cut(s) | 871, 1610 |
| SchI | GAGTC | 5 cut(s) | 146, 192, 514, 551, 1322 |
| ScrFI | CCNGG | 3 cut(s) | 797, 1011, 1538 |
| SduI | GDGCHC | 4 cut(s) | 225, 481, 1086, 1299 |
| SexAI | ACCWGGT | 1 cut(s) | 1009 |
| SfaNI | GCATC | 9 cut(s) | 66, 166, 195, 661, 798, 1135, 1164, 1381, 1408 |
| SfcI | CTRYAG | 1 cut(s) | 231 |
| SinI | GGWCC | 2 cut(s) | 871, 1610 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 74 |
| Sse9I | AATT | 7 cut(s) | 35, 336, 746, 1076, 1333, 1422, 1434 |
| SseBI | AGGCCT | 1 cut(s) | 95 |
| SsiI | CCGC | 5 cut(s) | 79, 824, 1054, 1351, 1566 |
| SspMI | CTAG | 2 cut(s) | 726, 884 |
| SstI | GAGCTC | 2 cut(s) | 481, 1086 |
| StuI | AGGCCT | 1 cut(s) | 95 |
| StyD4I | CCNGG | 3 cut(s) | 795, 1009, 1536 |
| StyI | CCWWGG | 4 cut(s) | 903, 947, 1106, 1236 |
| TaaI | ACNGT | 5 cut(s) | 112, 133, 937, 961, 1129 |
| TaiI | ACGT | 1 cut(s) | 1584 |
| TaqI | TCGA | 4 cut(s) | 252, 1137, 1248, 1629 |
| TasI | AATT | 7 cut(s) | 35, 336, 746, 1076, 1333, 1422, 1434 |
| TauI | GCSGC | 1 cut(s) | 1569 |
| TfiI | GAWTC | 3 cut(s) | 418, 730, 1021 |
| Tru1I | TTAA | 3 cut(s) | 198, 1362, 1633 |
| Tru9I | TTAA | 3 cut(s) | 198, 1362, 1633 |
| TscAI | CASTG | 6 cut(s) | 232, 274, 538, 697, 1192, 1606 |
| TseFI | GTSAC | 1 cut(s) | 889 |
| TseI | GCWGC | 9 cut(s) | 41, 157, 230, 233, 751, 821, 833, 1394, 1547 |
| Tsp45I | GTSAC | 1 cut(s) | 889 |
| TspDTI | ATGAA | 3 cut(s) | 576, 816, 1590 |
| TspRI | CASTG | 6 cut(s) | 232, 274, 538, 697, 1192, 1606 |
| VpaK11BI | GGWCC | 2 cut(s) | 871, 1610 |
| XapI | RAATTY | 1 cut(s) | 1076 |
| XceI | RCATGY | 1 cut(s) | 1351 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 1324 |
| XmnI | GAANNNNTTC | 1 cut(s) | 1581 |
| XspI | CTAG | 2 cut(s) | 726, 884 |