Heat shock cognate 70 kDa
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AciI | CCGC | 3 cut(s) | 36, 972, 1029 |
| AclWI | GGATC | 2 cut(s) | 438, 532 |
| AcoI | YGGCCR | 1 cut(s) | 786 |
| AcsI | RAATTY | 2 cut(s) | 410, 534 |
| AcuI | CTGAAG | 1 cut(s) | 1059 |
| AfaI | GTAC | 5 cut(s) | 118, 546, 633, 801, 1079 |
| AflII | CTTAAG | 1 cut(s) | 995 |
| AgsI | TTSAA | 4 cut(s) | 146, 236, 336, 416 |
| AjnI | CCWGG | 2 cut(s) | 901, 1092 |
| AluBI | AGCT | 7 cut(s) | 41, 170, 421, 553, 583, 638, 1118 |
| AluI | AGCT | 7 cut(s) | 41, 170, 421, 553, 583, 638, 1118 |
| Alw21I | GWGCWC | 3 cut(s) | 225, 423, 888 |
| Alw26I | GTCTC | 3 cut(s) | 446, 973, 1127 |
| Alw44I | GTGCAC | 1 cut(s) | 221 |
| AlwI | GGATC | 2 cut(s) | 438, 532 |
| AoxI | GGCC | 3 cut(s) | 66, 786, 1090 |
| ApaLI | GTGCAC | 1 cut(s) | 221 |
| ApeKI | GCWGC | 3 cut(s) | 38, 626, 638 |
| ApoI | RAATTY | 2 cut(s) | 410, 534 |
| AspS9I | GGNCC | 2 cut(s) | 905, 1090 |
| AsuHPI | GGTGA | 1 cut(s) | 209 |
| AvaII | GGWCC | 1 cut(s) | 905 |
| BaeGI | GKGCMC | 1 cut(s) | 225 |
| BalI | TGGCCA | 1 cut(s) | 788 |
| BanII | GRGCYC | 1 cut(s) | 423 |
| Bbv12I | GWGCWC | 3 cut(s) | 225, 423, 888 |
| BbvCI | CCTCAGC | 1 cut(s) | 975 |
| BbvI | GCAGC | 3 cut(s) | 50, 613, 625 |
| BccI | CCATC | 1 cut(s) | 999 |
| BceAI | ACGGC | 2 cut(s) | 87, 614 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 929, 963 |
| BciT130I | CCWGG | 2 cut(s) | 903, 1094 |
| BclI | TGATCA | 1 cut(s) | 1059 |
| BcoDI | GTCTC | 3 cut(s) | 446, 973, 1127 |
| BfaI | CTAG | 1 cut(s) | 531 |
| BfmI | CTRYAG | 1 cut(s) | 639 |
| BfrI | CTTAAG | 1 cut(s) | 995 |
| BglII | AGATCT | 1 cut(s) | 562 |
| BisI | GCNGC | 4 cut(s) | 36, 39, 627, 639 |
| BlsI | GCNGC | 4 cut(s) | 37, 40, 628, 640 |
| Bme1390I | CCNGG | 2 cut(s) | 903, 1094 |
| Bme18I | GGWCC | 1 cut(s) | 905 |
| BmgT120I | GGNCC | 2 cut(s) | 905, 1090 |
| BmrFI | CCNGG | 2 cut(s) | 903, 1094 |
| BmsI | GCATC | 3 cut(s) | 466, 603, 937 |
| BpmI | CTGGAG | 1 cut(s) | 198 |
| Bpu10I | CCTNAGC | 1 cut(s) | 975 |
| Bsa29I | ATCGAT | 1 cut(s) | 945 |
| BsaBI | GATNNNNATC | 1 cut(s) | 186 |
| BsaJI | CCNNGG | 3 cut(s) | 69, 540, 706 |
| Bse1I | ACTGG | 1 cut(s) | 260 |
| Bse3DI | GCAATG | 1 cut(s) | 277 |
| Bse8I | GATNNNNATC | 1 cut(s) | 186 |
| BseBI | CCWGG | 2 cut(s) | 903, 1094 |
| BseCI | ATCGAT | 1 cut(s) | 945 |
| BseDI | CCNNGG | 3 cut(s) | 69, 540, 706 |
| BseGI | GGATG | 1 cut(s) | 481 |
| BseJI | GATNNNNATC | 1 cut(s) | 186 |
| BseMI | GCAATG | 1 cut(s) | 277 |
| BseMII | CTCAG | 2 cut(s) | 278, 966 |
| BseNI | ACTGG | 1 cut(s) | 260 |
| BseSI | GKGCMC | 1 cut(s) | 225 |
| BseXI | GCAGC | 3 cut(s) | 50, 613, 625 |
| BsgI | GTGCAG | 1 cut(s) | 689 |
| BshFI | GGCC | 3 cut(s) | 68, 788, 1092 |
| BshVI | ATCGAT | 1 cut(s) | 945 |
| BsiHKAI | GWGCWC | 3 cut(s) | 225, 423, 888 |
| BsmAI | GTCTC | 3 cut(s) | 446, 973, 1127 |
| BsmBI | CGTCTC | 1 cut(s) | 1127 |
| BsnI | GGCC | 3 cut(s) | 68, 788, 1092 |
| Bsp1286I | GDGCHC | 3 cut(s) | 225, 423, 888 |
| Bsp143I | GATC | 6 cut(s) | 103, 430, 524, 562, 942, 1059 |
| BspACI | CCGC | 3 cut(s) | 36, 972, 1029 |
| BspANI | GGCC | 3 cut(s) | 68, 788, 1092 |
| BspCNI | CTCAG | 2 cut(s) | 279, 967 |
| BspDI | ATCGAT | 1 cut(s) | 945 |
| BspMAI | CTGCAG | 1 cut(s) | 643 |
| BspPI | GGATC | 2 cut(s) | 438, 532 |
| BspQI | GCTCTTC | 1 cut(s) | 411 |
| BspTI | CTTAAG | 1 cut(s) | 995 |
| BsrDI | GCAATG | 1 cut(s) | 277 |
| BsrI | ACTGG | 1 cut(s) | 260 |
| BssECI | CCNNGG | 3 cut(s) | 69, 540, 706 |
| BssMI | GATC | 6 cut(s) | 103, 430, 524, 562, 942, 1059 |
| BssT1I | CCWWGG | 2 cut(s) | 540, 706 |
| Bst2UI | CCWGG | 2 cut(s) | 903, 1094 |
| Bst4CI | ACNGT | 1 cut(s) | 457 |
| Bst6I | CTCTTC | 1 cut(s) | 411 |
| BstAFI | CTTAAG | 1 cut(s) | 995 |
| BstAPI | GCANNNNNTGC | 3 cut(s) | 44, 623, 883 |
| BstDEI | CTNAG | 3 cut(s) | 287, 975, 1119 |
| BstDSI | CCRYGG | 1 cut(s) | 69 |
| BstF5I | GGATG | 1 cut(s) | 481 |
| BstKTI | GATC | 6 cut(s) | 106, 433, 527, 565, 945, 1062 |
| BstMAI | GTCTC | 3 cut(s) | 446, 973, 1127 |
| BstMBI | GATC | 6 cut(s) | 103, 430, 524, 562, 942, 1059 |
| BstMWI | GCNNNNNNNGC | 5 cut(s) | 44, 623, 635, 883, 1124 |
| BstNI | CCWGG | 2 cut(s) | 903, 1094 |
| BstSCI | CCNGG | 2 cut(s) | 901, 1092 |
| BstSFI | CTRYAG | 1 cut(s) | 639 |
| BstSLI | GKGCMC | 1 cut(s) | 225 |
| BstV1I | GCAGC | 3 cut(s) | 50, 613, 625 |
| BstX2I | RGATCY | 3 cut(s) | 430, 524, 562 |
| BstYI | RGATCY | 3 cut(s) | 430, 524, 562 |
| Bsu15I | ATCGAT | 1 cut(s) | 945 |
| BsuRI | GGCC | 3 cut(s) | 68, 788, 1092 |
| BsuTUI | ATCGAT | 1 cut(s) | 945 |
| BtgI | CCRYGG | 1 cut(s) | 69 |
| BtgZI | GCGATG | 1 cut(s) | 1130 |
| BtsCI | GGATG | 1 cut(s) | 481 |
| BtsIMutI | CAGTG | 2 cut(s) | 253, 284 |
| Cfr13I | GGNCC | 2 cut(s) | 905, 1090 |
| ClaI | ATCGAT | 1 cut(s) | 945 |
| Csp6I | GTAC | 5 cut(s) | 117, 545, 632, 800, 1078 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 58, 93 |
| CviAII | CATG | 1 cut(s) | 427 |
| CviQI | GTAC | 5 cut(s) | 117, 545, 632, 800, 1078 |
| DdeI | CTNAG | 3 cut(s) | 287, 975, 1119 |
| DpnI | GATC | 6 cut(s) | 105, 432, 526, 564, 944, 1061 |
| DpnII | GATC | 6 cut(s) | 103, 430, 524, 562, 942, 1059 |
| EaeI | YGGCCR | 1 cut(s) | 786 |
| Eam1104I | CTCTTC | 1 cut(s) | 411 |
| EarI | CTCTTC | 1 cut(s) | 411 |
| Ecl136II | GAGCTC | 1 cut(s) | 421 |
| Eco130I | CCWWGG | 2 cut(s) | 540, 706 |
| Eco24I | GRGCYC | 1 cut(s) | 423 |
| Eco47I | GGWCC | 1 cut(s) | 905 |
| Eco53kI | GAGCTC | 1 cut(s) | 421 |
| Eco57I | CTGAAG | 1 cut(s) | 1059 |
| EcoICRI | GAGCTC | 1 cut(s) | 421 |
| EcoRI | GAATTC | 1 cut(s) | 534 |
| EcoRII | CCWGG | 2 cut(s) | 901, 1092 |
| EcoT14I | CCWWGG | 2 cut(s) | 540, 706 |
| EcoT38I | GRGCYC | 1 cut(s) | 423 |
| ErhI | CCWWGG | 2 cut(s) | 540, 706 |
| Esp3I | CGTCTC | 1 cut(s) | 1127 |
| FaeI | CATG | 1 cut(s) | 430 |
| FatI | CATG | 1 cut(s) | 426 |
| FauI | CCCGC | 1 cut(s) | 1022 |
| FauNDI | CATATG | 2 cut(s) | 49, 619 |
| FbaI | TGATCA | 1 cut(s) | 1059 |
| Fnu4HI | GCNGC | 4 cut(s) | 36, 39, 627, 639 |
| FokI | GGATG | 1 cut(s) | 488 |
| FriOI | GRGCYC | 1 cut(s) | 423 |
| Fsp4HI | GCNGC | 4 cut(s) | 36, 39, 627, 639 |
| FspBI | CTAG | 1 cut(s) | 531 |
| GluI | GCNGC | 4 cut(s) | 36, 39, 627, 639 |
| GsuI | CTGGAG | 1 cut(s) | 198 |
| HaeIII | GGCC | 3 cut(s) | 68, 788, 1092 |
| Hin1II | CATG | 1 cut(s) | 430 |
| HinfI | GANTC | 4 cut(s) | 325, 362, 894, 987 |
| HphI | GGTGA | 1 cut(s) | 209 |
| Hpy166II | GTNNAC | 1 cut(s) | 223 |
| Hpy188I | TCNGA | 1 cut(s) | 1039 |
| Hpy188III | TCNNGA | 5 cut(s) | 56, 236, 244, 602, 940 |
| Hpy8I | GTNNAC | 1 cut(s) | 223 |
| HpyAV | CCTTC | 4 cut(s) | 143, 253, 884, 997 |
| HpyCH4III | ACNGT | 1 cut(s) | 457 |
| HpyCH4V | TGCA | 8 cut(s) | 47, 223, 559, 588, 617, 641, 670, 1127 |
| HpyF10VI | GCNNNNNNNGC | 5 cut(s) | 44, 623, 635, 883, 1124 |
| HpyF3I | CTNAG | 3 cut(s) | 287, 975, 1119 |
| Hsp92II | CATG | 1 cut(s) | 430 |
| Ksp22I | TGATCA | 1 cut(s) | 1059 |
| Kzo9I | GATC | 6 cut(s) | 103, 430, 524, 562, 942, 1059 |
| LguI | GCTCTTC | 1 cut(s) | 411 |
| LmnI | GCTCC | 1 cut(s) | 580 |
| LpnPI | CCDG | 9 cut(s) | 162, 241, 615, 656, 711, 888, 915, 1079, 1106 |
| Lsp1109I | GCAGC | 3 cut(s) | 50, 613, 625 |
| LweI | GCATC | 3 cut(s) | 466, 603, 937 |
| MaeI | CTAG | 1 cut(s) | 531 |
| MaeIII | GTNAC | 2 cut(s) | 691, 1006 |
| MalI | GATC | 6 cut(s) | 105, 432, 526, 564, 944, 1061 |
| MboI | GATC | 6 cut(s) | 103, 430, 524, 562, 942, 1059 |
| MflI | RGATCY | 3 cut(s) | 430, 524, 562 |
| MhlI | GDGCHC | 3 cut(s) | 225, 423, 888 |
| MlsI | TGGCCA | 1 cut(s) | 788 |
| MluNI | TGGCCA | 1 cut(s) | 788 |
| MlyI | GAGTC | 2 cut(s) | 319, 981 |
| MnlI | CCTC | 9 cut(s) | 154, 367, 465, 829, 909, 930, 970, 1019, 1081 |
| Mox20I | TGGCCA | 1 cut(s) | 788 |
| MscI | TGGCCA | 1 cut(s) | 788 |
| MseI | TTAA | 4 cut(s) | 21, 138, 570, 996 |
| MslI | CAYNNNNRTG | 1 cut(s) | 192 |
| Msp20I | TGGCCA | 1 cut(s) | 788 |
| MspA1I | CMGCKG | 1 cut(s) | 974 |
| MspCI | CTTAAG | 1 cut(s) | 995 |
| MspR9I | CCNGG | 2 cut(s) | 903, 1094 |
| MvaI | CCWGG | 2 cut(s) | 903, 1094 |
| MwoI | GCNNNNNNNGC | 5 cut(s) | 44, 623, 635, 883, 1124 |
| NdeI | CATATG | 2 cut(s) | 49, 619 |
| NdeII | GATC | 6 cut(s) | 103, 430, 524, 562, 942, 1059 |
| NlaIII | CATG | 1 cut(s) | 430 |
| NmeAIII | GCCGAG | 1 cut(s) | 880 |
| PciSI | GCTCTTC | 1 cut(s) | 411 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 378 |
| PfeI | GAWTC | 2 cut(s) | 362, 894 |
| PkrI | GCNGC | 4 cut(s) | 37, 40, 628, 640 |
| PleI | GAGTC | 2 cut(s) | 319, 981 |
| PpsI | GAGTC | 2 cut(s) | 319, 981 |
| Psp124BI | GAGCTC | 1 cut(s) | 423 |
| Psp6I | CCWGG | 2 cut(s) | 901, 1092 |
| PspGI | CCWGG | 2 cut(s) | 901, 1092 |
| PspPI | GGNCC | 2 cut(s) | 905, 1090 |
| PstI | CTGCAG | 1 cut(s) | 643 |
| PsuI | RGATCY | 3 cut(s) | 430, 524, 562 |
| RsaI | GTAC | 5 cut(s) | 118, 546, 633, 801, 1079 |
| RsaNI | GTAC | 5 cut(s) | 117, 545, 632, 800, 1078 |
| RseI | CAYNNNNRTG | 1 cut(s) | 192 |
| SacI | GAGCTC | 1 cut(s) | 423 |
| SapI | GCTCTTC | 1 cut(s) | 411 |
| SaqAI | TTAA | 4 cut(s) | 21, 138, 570, 996 |
| SatI | GCNGC | 4 cut(s) | 36, 39, 627, 639 |
| Sau3AI | GATC | 6 cut(s) | 103, 430, 524, 562, 942, 1059 |
| Sau96I | GGNCC | 2 cut(s) | 905, 1090 |
| SchI | GAGTC | 2 cut(s) | 319, 981 |
| ScrFI | CCNGG | 2 cut(s) | 903, 1094 |
| SduI | GDGCHC | 3 cut(s) | 225, 423, 888 |
| SfaNI | GCATC | 3 cut(s) | 466, 603, 937 |
| SfcI | CTRYAG | 1 cut(s) | 639 |
| SinI | GGWCC | 1 cut(s) | 905 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 192 |
| SmlI | CTYRAG | 1 cut(s) | 995 |
| SmoI | CTYRAG | 1 cut(s) | 995 |
| SsiI | CCGC | 3 cut(s) | 36, 972, 1029 |
| SspMI | CTAG | 1 cut(s) | 531 |
| SstI | GAGCTC | 1 cut(s) | 423 |
| StyD4I | CCNGG | 2 cut(s) | 901, 1092 |
| StyI | CCWWGG | 2 cut(s) | 540, 706 |
| TaaI | ACNGT | 1 cut(s) | 457 |
| TaqI | TCGA | 7 cut(s) | 102, 243, 372, 381, 888, 939, 945 |
| TatI | WGTACW | 1 cut(s) | 1077 |
| TauI | GCSGC | 1 cut(s) | 38 |
| TfiI | GAWTC | 2 cut(s) | 362, 894 |
| Tru1I | TTAA | 4 cut(s) | 21, 138, 570, 996 |
| Tru9I | TTAA | 4 cut(s) | 21, 138, 570, 996 |
| TscAI | CASTG | 2 cut(s) | 260, 291 |
| TseI | GCWGC | 3 cut(s) | 38, 626, 638 |
| TspDTI | ATGAA | 3 cut(s) | 17, 621, 710 |
| TspGWI | ACGGA | 1 cut(s) | 1151 |
| TspRI | CASTG | 2 cut(s) | 260, 291 |
| Vha464I | CTTAAG | 1 cut(s) | 995 |
| VneI | GTGCAC | 1 cut(s) | 221 |
| VpaK11BI | GGWCC | 1 cut(s) | 905 |
| XapI | RAATTY | 2 cut(s) | 410, 534 |
| XspI | CTAG | 1 cut(s) | 531 |