Belongs to the heat shock protein 70 family
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 1203 |
| Acc36I | ACCTGC | 1 cut(s) | 460 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 1460 |
| AccII | CGCG | 1 cut(s) | 84 |
| AciI | CCGC | 5 cut(s) | 82, 170, 223, 780, 1127 |
| AclWI | GGATC | 1 cut(s) | 948 |
| AcoI | YGGCCR | 1 cut(s) | 171 |
| AcsI | RAATTY | 1 cut(s) | 1180 |
| AcuI | CTGAAG | 1 cut(s) | 1350 |
| AcyI | GRCGYC | 1 cut(s) | 122 |
| AfaI | GTAC | 2 cut(s) | 450, 1136 |
| AfiI | CCNNNNNNNGG | 4 cut(s) | 29, 1418, 1457, 1460 |
| AgsI | TTSAA | 9 cut(s) | 286, 463, 656, 754, 903, 998, 1274, 1298, 1487 |
| AjnI | CCWGG | 3 cut(s) | 180, 301, 1185 |
| AluBI | AGCT | 8 cut(s) | 274, 794, 815, 931, 1156, 1246, 1318, 1357 |
| AluI | AGCT | 8 cut(s) | 274, 794, 815, 931, 1156, 1246, 1318, 1357 |
| Alw21I | GWGCWC | 4 cut(s) | 522, 796, 1158, 1359 |
| AlwI | GGATC | 1 cut(s) | 948 |
| Ama87I | CYCGRG | 1 cut(s) | 416 |
| AoxI | GGCC | 5 cut(s) | 171, 278, 298, 508, 1048 |
| ApeKI | GCWGC | 6 cut(s) | 73, 356, 545, 548, 1253, 1406 |
| ApoI | RAATTY | 1 cut(s) | 1180 |
| ArsI | GACNNNNNNTTYG | 4 cut(s) | 863, 895, 1009, 1041 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 1446 |
| AspLEI | GCGC | 1 cut(s) | 1406 |
| AspS9I | GGNCC | 3 cut(s) | 20, 299, 1475 |
| AsuHPI | GGTGA | 7 cut(s) | 257, 317, 685, 719, 742, 901, 1360 |
| AsuII | TTCGAA | 1 cut(s) | 924 |
| AvaI | CYCGRG | 1 cut(s) | 416 |
| AvaII | GGWCC | 2 cut(s) | 20, 1475 |
| BanII | GRGCYC | 3 cut(s) | 796, 1158, 1359 |
| BbsI | GAAGAC | 2 cut(s) | 1093, 1152 |
| Bbv12I | GWGCWC | 4 cut(s) | 522, 796, 1158, 1359 |
| BbvCI | CCTCAGC | 1 cut(s) | 1323 |
| BbvI | GCAGC | 6 cut(s) | 85, 343, 532, 535, 1265, 1393 |
| BccI | CCATC | 6 cut(s) | 721, 869, 914, 1076, 1108, 1385 |
| BceAI | ACGGC | 2 cut(s) | 109, 1324 |
| BciT130I | CCWGG | 3 cut(s) | 182, 303, 1187 |
| BfaI | CTAG | 1 cut(s) | 1304 |
| BfmI | CTRYAG | 3 cut(s) | 52, 357, 1044 |
| BfoI | RGCGCY | 1 cut(s) | 1407 |
| BfuAI | ACCTGC | 1 cut(s) | 460 |
| BisI | GCNGC | 7 cut(s) | 74, 171, 357, 546, 549, 1254, 1407 |
| BlsI | GCNGC | 7 cut(s) | 75, 172, 358, 547, 550, 1255, 1408 |
| Bme1390I | CCNGG | 3 cut(s) | 182, 303, 1187 |
| Bme18I | GGWCC | 2 cut(s) | 20, 1475 |
| BmeT110I | CYCGRG | 1 cut(s) | 416 |
| BmgT120I | GGNCC | 3 cut(s) | 20, 299, 1475 |
| BmiI | GGNNCC | 4 cut(s) | 21, 628, 963, 1477 |
| BmrFI | CCNGG | 3 cut(s) | 182, 303, 1187 |
| BmsI | GCATC | 5 cut(s) | 157, 205, 381, 481, 1267 |
| BpiI | GAAGAC | 2 cut(s) | 1093, 1152 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 857, 889 |
| BpmI | CTGGAG | 2 cut(s) | 708, 1208 |
| Bpu10I | CCTNAGC | 1 cut(s) | 1323 |
| Bpu14I | TTCGAA | 1 cut(s) | 924 |
| BpuEI | CTTGAG | 1 cut(s) | 1142 |
| Bsa29I | ATCGAT | 1 cut(s) | 36 |
| BsaBI | GATNNNNATC | 1 cut(s) | 32 |
| BsaHI | GRCGYC | 1 cut(s) | 122 |
| BsaJI | CCNNGG | 3 cut(s) | 301, 1095, 1185 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 229, 259 |
| Bsc4I | CCNNNNNNNGG | 4 cut(s) | 29, 1418, 1457, 1460 |
| Bse118I | RCCGGY | 2 cut(s) | 22, 296 |
| Bse1I | ACTGG | 3 cut(s) | 582, 965, 1462 |
| Bse3DI | GCAATG | 3 cut(s) | 501, 552, 1380 |
| Bse8I | GATNNNNATC | 1 cut(s) | 32 |
| BseBI | CCWGG | 3 cut(s) | 182, 303, 1187 |
| BseCI | ATCGAT | 1 cut(s) | 36 |
| BseDI | CCNNGG | 3 cut(s) | 301, 1095, 1185 |
| BseGI | GGATG | 5 cut(s) | 496, 732, 842, 1231, 1444 |
| BseJI | GATNNNNATC | 1 cut(s) | 32 |
| BseLI | CCNNNNNNNGG | 4 cut(s) | 29, 1418, 1457, 1460 |
| BseMI | GCAATG | 3 cut(s) | 501, 552, 1380 |
| BseMII | CTCAG | 4 cut(s) | 141, 485, 866, 1314 |
| BseNI | ACTGG | 3 cut(s) | 582, 965, 1462 |
| BseRI | GAGGAG | 5 cut(s) | 241, 356, 636, 1115, 1166 |
| BseXI | GCAGC | 6 cut(s) | 85, 343, 532, 535, 1265, 1393 |
| BseYI | CCCAGC | 1 cut(s) | 494 |
| BsgI | GTGCAG | 1 cut(s) | 1272 |
| Bsh1236I | CGCG | 1 cut(s) | 84 |
| Bsh1285I | CGRYCG | 2 cut(s) | 30, 82 |
| BshFI | GGCC | 5 cut(s) | 173, 280, 300, 510, 1050 |
| BshVI | ATCGAT | 1 cut(s) | 36 |
| BsiEI | CGRYCG | 2 cut(s) | 30, 82 |
| BsiHKAI | GWGCWC | 4 cut(s) | 522, 796, 1158, 1359 |
| BsiHKCI | CYCGRG | 1 cut(s) | 416 |
| BsiSI | CCGG | 2 cut(s) | 23, 297 |
| BslFI | GGGAC | 3 cut(s) | 33, 433, 1461 |
| BslI | CCNNNNNNNGG | 4 cut(s) | 29, 1418, 1457, 1460 |
| BsmFI | GGGAC | 3 cut(s) | 33, 433, 1461 |
| BsmI | GAATGC | 1 cut(s) | 439 |
| BsnI | GGCC | 5 cut(s) | 173, 280, 300, 510, 1050 |
| BsoBI | CYCGRG | 1 cut(s) | 416 |
| Bsp119I | TTCGAA | 1 cut(s) | 924 |
| Bsp1286I | GDGCHC | 4 cut(s) | 522, 796, 1158, 1359 |
| Bsp143I | GATC | 7 cut(s) | 27, 37, 90, 103, 159, 613, 940 |
| BspACI | CCGC | 5 cut(s) | 82, 170, 223, 780, 1127 |
| BspANI | GGCC | 5 cut(s) | 173, 280, 300, 510, 1050 |
| BspCNI | CTCAG | 4 cut(s) | 142, 484, 865, 1315 |
| BspDI | ATCGAT | 1 cut(s) | 36 |
| BspFNI | CGCG | 1 cut(s) | 84 |
| BspHI | TCATGA | 1 cut(s) | 1030 |
| BspLI | GGNNCC | 4 cut(s) | 21, 628, 963, 1477 |
| BspMAI | CTGCAG | 1 cut(s) | 361 |
| BspMI | ACCTGC | 1 cut(s) | 460 |
| BspPI | GGATC | 1 cut(s) | 948 |
| BspQI | GCTCTTC | 1 cut(s) | 921 |
| BspT104I | TTCGAA | 1 cut(s) | 924 |
| BsrDI | GCAATG | 3 cut(s) | 501, 552, 1380 |
| BsrFI | RCCGGY | 2 cut(s) | 22, 296 |
| BsrI | ACTGG | 3 cut(s) | 582, 965, 1462 |
| BssAI | RCCGGY | 2 cut(s) | 22, 296 |
| BssECI | CCNNGG | 3 cut(s) | 301, 1095, 1185 |
| BssMI | GATC | 7 cut(s) | 27, 37, 90, 103, 159, 613, 940 |
| BssNI | GRCGYC | 1 cut(s) | 122 |
| BssT1I | CCWWGG | 1 cut(s) | 1095 |
| Bst2UI | CCWGG | 3 cut(s) | 182, 303, 1187 |
| Bst4CI | ACNGT | 4 cut(s) | 56, 208, 427, 448 |
| Bst6I | CTCTTC | 3 cut(s) | 648, 921, 1326 |
| BstACI | GRCGYC | 1 cut(s) | 122 |
| BstBI | TTCGAA | 1 cut(s) | 924 |
| BstC8I | GCNNGC | 5 cut(s) | 298, 480, 508, 1290, 1320 |
| BstDEI | CTNAG | 5 cut(s) | 150, 471, 797, 852, 1323 |
| BstF5I | GGATG | 5 cut(s) | 496, 732, 842, 1231, 1444 |
| BstFNI | CGCG | 1 cut(s) | 84 |
| BstH2I | RGCGCY | 1 cut(s) | 1407 |
| BstHHI | GCGC | 1 cut(s) | 1406 |
| BstKTI | GATC | 7 cut(s) | 30, 40, 93, 106, 162, 616, 943 |
| BstMBI | GATC | 7 cut(s) | 27, 37, 90, 103, 159, 613, 940 |
| BstMCI | CGRYCG | 2 cut(s) | 30, 82 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 554 |
| BstNI | CCWGG | 3 cut(s) | 182, 303, 1187 |
| BstSCI | CCNGG | 3 cut(s) | 180, 301, 1185 |
| BstSFI | CTRYAG | 3 cut(s) | 52, 357, 1044 |
| BstUI | CGCG | 1 cut(s) | 84 |
| BstV1I | GCAGC | 6 cut(s) | 85, 343, 532, 535, 1265, 1393 |
| BstV2I | GAAGAC | 2 cut(s) | 1093, 1152 |
| BstX2I | RGATCY | 1 cut(s) | 940 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 704 |
| BstYI | RGATCY | 1 cut(s) | 940 |
| Bsu15I | ATCGAT | 1 cut(s) | 36 |
| BsuRI | GGCC | 5 cut(s) | 173, 280, 300, 510, 1050 |
| BsuTUI | ATCGAT | 1 cut(s) | 36 |
| BtgZI | GCGATG | 2 cut(s) | 79, 180 |
| BtsCI | GGATG | 5 cut(s) | 496, 732, 842, 1231, 1444 |
| BtsI | GCAGTG | 2 cut(s) | 846, 1471 |
| BtsIMutI | CAGTG | 5 cut(s) | 141, 432, 589, 846, 1471 |
| BveI | ACCTGC | 1 cut(s) | 460 |
| Cac8I | GCNNGC | 5 cut(s) | 298, 480, 508, 1290, 1320 |
| CciI | TCATGA | 1 cut(s) | 1030 |
| CfoI | GCGC | 1 cut(s) | 1406 |
| Cfr10I | RCCGGY | 2 cut(s) | 22, 296 |
| Cfr13I | GGNCC | 3 cut(s) | 20, 299, 1475 |
| ClaI | ATCGAT | 1 cut(s) | 36 |
| CseI | GACGC | 3 cut(s) | 49, 73, 130 |
| CsiI | ACCWGGT | 1 cut(s) | 180 |
| Csp6I | GTAC | 2 cut(s) | 449, 1135 |
| CviAII | CATG | 8 cut(s) | 190, 268, 316, 937, 1031, 1207, 1417, 1435 |
| CviQI | GTAC | 2 cut(s) | 449, 1135 |
| DdeI | CTNAG | 5 cut(s) | 150, 471, 797, 852, 1323 |
| DpnI | GATC | 7 cut(s) | 29, 39, 92, 105, 161, 615, 942 |
| DpnII | GATC | 7 cut(s) | 27, 37, 90, 103, 159, 613, 940 |
| EaeI | YGGCCR | 1 cut(s) | 171 |
| Eam1104I | CTCTTC | 3 cut(s) | 648, 921, 1326 |
| EarI | CTCTTC | 3 cut(s) | 648, 921, 1326 |
| Ecl136II | GAGCTC | 3 cut(s) | 794, 1156, 1357 |
| Eco130I | CCWWGG | 1 cut(s) | 1095 |
| Eco24I | GRGCYC | 3 cut(s) | 796, 1158, 1359 |
| Eco47I | GGWCC | 2 cut(s) | 20, 1475 |
| Eco53kI | GAGCTC | 3 cut(s) | 794, 1156, 1357 |
| Eco57I | CTGAAG | 1 cut(s) | 1350 |
| Eco88I | CYCGRG | 1 cut(s) | 416 |
| EcoICRI | GAGCTC | 3 cut(s) | 794, 1156, 1357 |
| EcoRII | CCWGG | 3 cut(s) | 180, 301, 1185 |
| EcoT14I | CCWWGG | 1 cut(s) | 1095 |
| EcoT38I | GRGCYC | 3 cut(s) | 796, 1158, 1359 |
| ErhI | CCWWGG | 1 cut(s) | 1095 |
| FaeI | CATG | 8 cut(s) | 193, 271, 319, 940, 1034, 1210, 1420, 1438 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 587, 619 |
| FaqI | GGGAC | 3 cut(s) | 33, 433, 1461 |
| FatI | CATG | 8 cut(s) | 189, 267, 315, 936, 1030, 1206, 1416, 1434 |
| Fnu4HI | GCNGC | 7 cut(s) | 74, 171, 357, 546, 549, 1254, 1407 |
| FokI | GGATG | 5 cut(s) | 503, 739, 829, 1238, 1451 |
| FriOI | GRGCYC | 3 cut(s) | 796, 1158, 1359 |
| Fsp4HI | GCNGC | 7 cut(s) | 74, 171, 357, 546, 549, 1254, 1407 |
| FspBI | CTAG | 1 cut(s) | 1304 |
| GlaI | GCGC | 1 cut(s) | 1405 |
| GluI | GCNGC | 7 cut(s) | 74, 171, 357, 546, 549, 1254, 1407 |
| GsaI | CCCAGC | 1 cut(s) | 498 |
| GsuI | CTGGAG | 2 cut(s) | 708, 1208 |
| HaeII | RGCGCY | 1 cut(s) | 1407 |
| HaeIII | GGCC | 5 cut(s) | 173, 280, 300, 510, 1050 |
| HapII | CCGG | 2 cut(s) | 23, 297 |
| HgaI | GACGC | 3 cut(s) | 49, 73, 130 |
| HhaI | GCGC | 1 cut(s) | 1406 |
| Hin1I | GRCGYC | 1 cut(s) | 122 |
| Hin1II | CATG | 8 cut(s) | 193, 271, 319, 940, 1034, 1210, 1420, 1438 |
| Hin6I | GCGC | 1 cut(s) | 1404 |
| HinP1I | GCGC | 1 cut(s) | 1404 |
| HincII | GTYRAC | 1 cut(s) | 202 |
| HindII | GTYRAC | 1 cut(s) | 202 |
| HindIII | AAGCTT | 1 cut(s) | 1244 |
| HinfI | GANTC | 8 cut(s) | 33, 248, 467, 733, 835, 872, 921, 1172 |
| HpaII | CCGG | 2 cut(s) | 23, 297 |
| HphI | GGTGA | 7 cut(s) | 257, 317, 685, 719, 742, 901, 1360 |
| Hpy166II | GTNNAC | 2 cut(s) | 141, 202 |
| Hpy188I | TCNGA | 7 cut(s) | 8, 32, 44, 65, 474, 855, 1144 |
| Hpy8I | GTNNAC | 2 cut(s) | 141, 202 |
| Hpy99I | CGWCG | 3 cut(s) | 65, 89, 124 |
| HpyAV | CCTTC | 6 cut(s) | 568, 653, 670, 1159, 1345, 1355 |
| HpyCH4III | ACNGT | 4 cut(s) | 56, 208, 427, 448 |
| HpyCH4IV | ACGT | 1 cut(s) | 132 |
| HpyCH4V | TGCA | 8 cut(s) | 359, 404, 986, 1015, 1253, 1280, 1371, 1385 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 554 |
| HpyF3I | CTNAG | 5 cut(s) | 150, 471, 797, 852, 1323 |
| HpySE526I | ACGT | 1 cut(s) | 132 |
| Hsp92I | GRCGYC | 1 cut(s) | 122 |
| Hsp92II | CATG | 8 cut(s) | 193, 271, 319, 940, 1034, 1210, 1420, 1438 |
| HspAI | GCGC | 1 cut(s) | 1404 |
| KroI | GCCGGC | 1 cut(s) | 296 |
| KroNI | GCCGGC | 1 cut(s) | 298 |
| Kzo9I | GATC | 7 cut(s) | 27, 37, 90, 103, 159, 613, 940 |
| LguI | GCTCTTC | 1 cut(s) | 921 |
| LmnI | GCTCC | 5 cut(s) | 525, 961, 1153, 1354, 1414 |
| Lsp1109I | GCAGC | 6 cut(s) | 85, 343, 532, 535, 1265, 1393 |
| LweI | GCATC | 5 cut(s) | 157, 205, 381, 481, 1267 |
| MabI | ACCWGGT | 1 cut(s) | 180 |
| MaeI | CTAG | 1 cut(s) | 1304 |
| MaeII | ACGT | 1 cut(s) | 132 |
| MaeIII | GTNAC | 4 cut(s) | 245, 305, 325, 442 |
| MalI | GATC | 7 cut(s) | 29, 39, 92, 105, 161, 615, 942 |
| MboI | GATC | 7 cut(s) | 27, 37, 90, 103, 159, 613, 940 |
| MfeI | CAATTG | 3 cut(s) | 651, 978, 1281 |
| MflI | RGATCY | 1 cut(s) | 940 |
| MhlI | GDGCHC | 4 cut(s) | 522, 796, 1158, 1359 |
| MlyI | GAGTC | 5 cut(s) | 242, 461, 829, 866, 1181 |
| MmeI | TCCRAC | 3 cut(s) | 43, 1122, 1478 |
| MroNI | GCCGGC | 1 cut(s) | 296 |
| MroXI | GAANNNNTTC | 1 cut(s) | 1446 |
| MseI | TTAA | 3 cut(s) | 227, 1221, 1498 |
| MslI | CAYNNNNRTG | 2 cut(s) | 702, 1421 |
| MspA1I | CMGCKG | 1 cut(s) | 780 |
| MspI | CCGG | 2 cut(s) | 23, 297 |
| MspR9I | CCNGG | 3 cut(s) | 182, 303, 1187 |
| MunI | CAATTG | 3 cut(s) | 651, 978, 1281 |
| Mva1269I | GAATGC | 1 cut(s) | 439 |
| MvaI | CCWGG | 3 cut(s) | 182, 303, 1187 |
| MvnI | CGCG | 1 cut(s) | 84 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 554 |
| NaeI | GCCGGC | 1 cut(s) | 298 |
| NdeII | GATC | 7 cut(s) | 27, 37, 90, 103, 159, 613, 940 |
| NgoMIV | GCCGGC | 1 cut(s) | 296 |
| NlaIII | CATG | 8 cut(s) | 193, 271, 319, 940, 1034, 1210, 1420, 1438 |
| NlaIV | GGNNCC | 4 cut(s) | 21, 628, 963, 1477 |
| NmuCI | GTSAC | 2 cut(s) | 245, 305 |
| NspV | TTCGAA | 1 cut(s) | 924 |
| PagI | TCATGA | 1 cut(s) | 1030 |
| PciSI | GCTCTTC | 1 cut(s) | 921 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 84 |
| PctI | GAATGC | 1 cut(s) | 439 |
| PdiI | GCCGGC | 1 cut(s) | 298 |
| PdmI | GAANNNNTTC | 1 cut(s) | 1446 |
| PfeI | GAWTC | 3 cut(s) | 33, 733, 921 |
| PflFI | GACNNNGTC | 2 cut(s) | 83, 124 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 1460 |
| PkrI | GCNGC | 7 cut(s) | 75, 172, 358, 547, 550, 1255, 1408 |
| Ple19I | CGATCG | 1 cut(s) | 30 |
| PleI | GAGTC | 5 cut(s) | 242, 461, 829, 866, 1180 |
| PpsI | GAGTC | 5 cut(s) | 242, 461, 829, 866, 1180 |
| PsiI | TTATAA | 1 cut(s) | 1203 |
| Psp124BI | GAGCTC | 3 cut(s) | 796, 1158, 1359 |
| Psp6I | CCWGG | 3 cut(s) | 180, 301, 1185 |
| PspFI | CCCAGC | 1 cut(s) | 494 |
| PspGI | CCWGG | 3 cut(s) | 180, 301, 1185 |
| PspN4I | GGNNCC | 4 cut(s) | 21, 628, 963, 1477 |
| PspPI | GGNCC | 3 cut(s) | 20, 299, 1475 |
| PstI | CTGCAG | 1 cut(s) | 361 |
| PsuI | RGATCY | 1 cut(s) | 940 |
| PsyI | GACNNNGTC | 2 cut(s) | 83, 124 |
| PvuI | CGATCG | 1 cut(s) | 30 |
| RsaI | GTAC | 2 cut(s) | 450, 1136 |
| RsaNI | GTAC | 2 cut(s) | 449, 1135 |
| RseI | CAYNNNNRTG | 2 cut(s) | 702, 1421 |
| SacI | GAGCTC | 3 cut(s) | 796, 1158, 1359 |
| SapI | GCTCTTC | 1 cut(s) | 921 |
| SaqAI | TTAA | 3 cut(s) | 227, 1221, 1498 |
| SatI | GCNGC | 7 cut(s) | 74, 171, 357, 546, 549, 1254, 1407 |
| Sau3AI | GATC | 7 cut(s) | 27, 37, 90, 103, 159, 613, 940 |
| Sau96I | GGNCC | 3 cut(s) | 20, 299, 1475 |
| SchI | GAGTC | 5 cut(s) | 242, 461, 829, 866, 1181 |
| ScrFI | CCNGG | 3 cut(s) | 182, 303, 1187 |
| SduI | GDGCHC | 4 cut(s) | 522, 796, 1158, 1359 |
| SexAI | ACCWGGT | 1 cut(s) | 180 |
| SfaNI | GCATC | 5 cut(s) | 157, 205, 381, 481, 1267 |
| SfcI | CTRYAG | 3 cut(s) | 52, 357, 1044 |
| SfuI | TTCGAA | 1 cut(s) | 924 |
| SinI | GGWCC | 2 cut(s) | 20, 1475 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 702, 1421 |
| SmlI | CTYRAG | 1 cut(s) | 1157 |
| SmoI | CTYRAG | 1 cut(s) | 1157 |
| SsiI | CCGC | 5 cut(s) | 82, 170, 223, 780, 1127 |
| SspMI | CTAG | 1 cut(s) | 1304 |
| SstI | GAGCTC | 3 cut(s) | 796, 1158, 1359 |
| StyD4I | CCNGG | 3 cut(s) | 180, 301, 1185 |
| StyI | CCWWGG | 1 cut(s) | 1095 |
| TaaI | ACNGT | 4 cut(s) | 56, 208, 427, 448 |
| TaiI | ACGT | 1 cut(s) | 135 |
| TaqI | TCGA | 9 cut(s) | 36, 87, 213, 669, 864, 891, 924, 1359, 1494 |
| TatI | WGTACW | 1 cut(s) | 1134 |
| TauI | GCSGC | 1 cut(s) | 173 |
| TfiI | GAWTC | 3 cut(s) | 33, 733, 921 |
| Tru1I | TTAA | 3 cut(s) | 227, 1221, 1498 |
| Tru9I | TTAA | 3 cut(s) | 227, 1221, 1498 |
| TscAI | CASTG | 5 cut(s) | 148, 432, 589, 853, 1471 |
| TseFI | GTSAC | 2 cut(s) | 245, 305 |
| TseI | GCWGC | 6 cut(s) | 73, 356, 545, 548, 1253, 1406 |
| Tsp45I | GTSAC | 2 cut(s) | 245, 305 |
| TspDTI | ATGAA | 3 cut(s) | 206, 284, 1455 |
| TspGWI | ACGGA | 1 cut(s) | 512 |
| TspRI | CASTG | 5 cut(s) | 148, 432, 589, 853, 1471 |
| Tth111I | GACNNNGTC | 2 cut(s) | 83, 124 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 1460 |
| VpaK11BI | GGWCC | 2 cut(s) | 20, 1475 |
| XapI | RAATTY | 1 cut(s) | 1180 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 1183 |
| XmnI | GAANNNNTTC | 1 cut(s) | 1446 |
| XspI | CTAG | 1 cut(s) | 1304 |