mediator of RNA polymerase II transcription subunit
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 443 |
| Acc65I | GGTACC | 1 cut(s) | 18 |
| AccB1I | GGYRCC | 1 cut(s) | 18 |
| AccI | GTMKAC | 1 cut(s) | 771 |
| AccII | CGCG | 1 cut(s) | 915 |
| AciI | CCGC | 4 cut(s) | 198, 453, 546, 913 |
| AclWI | GGATC | 1 cut(s) | 427 |
| AcoI | YGGCCR | 2 cut(s) | 3, 278 |
| AcsI | RAATTY | 1 cut(s) | 920 |
| AcuI | CTGAAG | 3 cut(s) | 381, 931, 1177 |
| AfaI | GTAC | 3 cut(s) | 20, 628, 1274 |
| AflIII | ACRYGT | 1 cut(s) | 1524 |
| AjnI | CCWGG | 2 cut(s) | 882, 1200 |
| AluBI | AGCT | 6 cut(s) | 494, 551, 656, 1093, 1139, 1148 |
| AluI | AGCT | 6 cut(s) | 494, 551, 656, 1093, 1139, 1148 |
| Alw26I | GTCTC | 3 cut(s) | 684, 1202, 1516 |
| AlwI | GGATC | 1 cut(s) | 427 |
| AoxI | GGCC | 4 cut(s) | 3, 278, 508, 678 |
| ApeKI | GCWGC | 6 cut(s) | 170, 472, 503, 548, 1136, 1148 |
| ApoI | RAATTY | 1 cut(s) | 920 |
| AseI | ATTAAT | 1 cut(s) | 1107 |
| Asp718I | GGTACC | 1 cut(s) | 18 |
| AspLEI | GCGC | 2 cut(s) | 359, 917 |
| AspS9I | GGNCC | 1 cut(s) | 379 |
| AsuHPI | GGTGA | 5 cut(s) | 317, 319, 719, 1201, 1394 |
| AvaII | GGWCC | 1 cut(s) | 379 |
| BalI | TGGCCA | 1 cut(s) | 280 |
| BanI | GGYRCC | 1 cut(s) | 18 |
| BauI | CACGAG | 1 cut(s) | 55 |
| BbsI | GAAGAC | 1 cut(s) | 717 |
| BbvCI | CCTCAGC | 1 cut(s) | 1518 |
| BbvI | GCAGC | 6 cut(s) | 182, 484, 490, 560, 1135, 1148 |
| BccI | CCATC | 2 cut(s) | 133, 1319 |
| BceAI | ACGGC | 1 cut(s) | 11 |
| BciT130I | CCWGG | 2 cut(s) | 884, 1202 |
| BclI | TGATCA | 1 cut(s) | 103 |
| BcoDI | GTCTC | 3 cut(s) | 684, 1202, 1516 |
| BfaI | CTAG | 4 cut(s) | 605, 1041, 1187, 1600 |
| BfoI | RGCGCY | 1 cut(s) | 360 |
| BglII | AGATCT | 1 cut(s) | 940 |
| BisI | GCNGC | 7 cut(s) | 171, 473, 504, 546, 549, 1137, 1149 |
| BlpI | GCTNAGC | 1 cut(s) | 150 |
| BlsI | GCNGC | 7 cut(s) | 172, 474, 505, 547, 550, 1138, 1150 |
| Bme1390I | CCNGG | 2 cut(s) | 884, 1202 |
| Bme18I | GGWCC | 1 cut(s) | 379 |
| BmgT120I | GGNCC | 1 cut(s) | 379 |
| BmiI | GGNNCC | 3 cut(s) | 20, 963, 1290 |
| BmrFI | CCNGG | 2 cut(s) | 884, 1202 |
| BmsI | GCATC | 6 cut(s) | 136, 157, 510, 825, 976, 1480 |
| BoxI | GACNNNNGTC | 1 cut(s) | 1194 |
| BpiI | GAAGAC | 1 cut(s) | 717 |
| BpmI | CTGGAG | 2 cut(s) | 708, 1428 |
| Bpu10I | CCTNAGC | 1 cut(s) | 1518 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 150 |
| BpuEI | CTTGAG | 1 cut(s) | 1525 |
| BsaBI | GATNNNNATC | 1 cut(s) | 1553 |
| BsaJI | CCNNGG | 7 cut(s) | 375, 415, 700, 789, 883, 1099, 1530 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 229, 259 |
| Bse118I | RCCGGY | 1 cut(s) | 506 |
| Bse1I | ACTGG | 2 cut(s) | 784, 1445 |
| Bse8I | GATNNNNATC | 1 cut(s) | 1553 |
| BseBI | CCWGG | 2 cut(s) | 884, 1202 |
| BseDI | CCNNGG | 7 cut(s) | 375, 415, 700, 789, 883, 1099, 1530 |
| BseGI | GGATG | 6 cut(s) | 371, 640, 840, 1078, 1210, 1597 |
| BseJI | GATNNNNATC | 1 cut(s) | 1553 |
| BseMII | CTCAG | 3 cut(s) | 141, 396, 1509 |
| BseNI | ACTGG | 2 cut(s) | 784, 1445 |
| BseXI | GCAGC | 6 cut(s) | 182, 484, 490, 560, 1135, 1148 |
| BsgI | GTGCAG | 3 cut(s) | 449, 863, 1155 |
| Bsh1236I | CGCG | 1 cut(s) | 915 |
| BshFI | GGCC | 4 cut(s) | 5, 280, 510, 680 |
| BshNI | GGYRCC | 1 cut(s) | 18 |
| BsiSI | CCGG | 1 cut(s) | 507 |
| BslFI | GGGAC | 2 cut(s) | 58, 434 |
| BsmAI | GTCTC | 3 cut(s) | 684, 1202, 1516 |
| BsmFI | GGGAC | 2 cut(s) | 58, 434 |
| BsmI | GAATGC | 2 cut(s) | 439, 1354 |
| BsnI | GGCC | 4 cut(s) | 5, 280, 510, 680 |
| Bsp143I | GATC | 4 cut(s) | 90, 103, 419, 940 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 150 |
| Bsp19I | CCATGG | 1 cut(s) | 375 |
| BspACI | CCGC | 4 cut(s) | 198, 453, 546, 913 |
| BspANI | GGCC | 4 cut(s) | 5, 280, 510, 680 |
| BspCNI | CTCAG | 3 cut(s) | 142, 397, 1510 |
| BspFNI | CGCG | 1 cut(s) | 915 |
| BspLI | GGNNCC | 3 cut(s) | 20, 963, 1290 |
| BspPI | GGATC | 1 cut(s) | 427 |
| BspQI | GCTCTTC | 1 cut(s) | 1042 |
| BspT107I | GGYRCC | 1 cut(s) | 18 |
| BsrFI | RCCGGY | 1 cut(s) | 506 |
| BsrI | ACTGG | 2 cut(s) | 784, 1445 |
| BssAI | RCCGGY | 1 cut(s) | 506 |
| BssECI | CCNNGG | 7 cut(s) | 375, 415, 700, 789, 883, 1099, 1530 |
| BssMI | GATC | 4 cut(s) | 90, 103, 419, 940 |
| BssSI | CACGAG | 1 cut(s) | 55 |
| BssT1I | CCWWGG | 5 cut(s) | 375, 415, 700, 789, 1099 |
| Bst2BI | CACGAG | 1 cut(s) | 55 |
| Bst2UI | CCWGG | 2 cut(s) | 884, 1202 |
| Bst4CI | ACNGT | 3 cut(s) | 427, 448, 1562 |
| Bst6I | CTCTTC | 2 cut(s) | 950, 1042 |
| BstAPI | GCANNNNNTGC | 2 cut(s) | 554, 1133 |
| BstC8I | GCNNGC | 1 cut(s) | 508 |
| BstDEI | CTNAG | 4 cut(s) | 150, 405, 797, 1518 |
| BstDSI | CCRYGG | 2 cut(s) | 375, 1530 |
| BstEII | GGTNACC | 1 cut(s) | 325 |
| BstF5I | GGATG | 6 cut(s) | 371, 640, 840, 1078, 1210, 1597 |
| BstFNI | CGCG | 1 cut(s) | 915 |
| BstH2I | RGCGCY | 1 cut(s) | 360 |
| BstHHI | GCGC | 2 cut(s) | 359, 917 |
| BstKTI | GATC | 4 cut(s) | 93, 106, 422, 943 |
| BstMAI | GTCTC | 3 cut(s) | 684, 1202, 1516 |
| BstMBI | GATC | 4 cut(s) | 90, 103, 419, 940 |
| BstMWI | GCNNNNNNNGC | 4 cut(s) | 500, 554, 1133, 1145 |
| BstNI | CCWGG | 2 cut(s) | 884, 1202 |
| BstNSI | RCATGY | 2 cut(s) | 819, 1528 |
| BstPAI | GACNNNNGTC | 1 cut(s) | 1194 |
| BstPI | GGTNACC | 1 cut(s) | 325 |
| BstSCI | CCNGG | 2 cut(s) | 882, 1200 |
| BstUI | CGCG | 1 cut(s) | 915 |
| BstV1I | GCAGC | 6 cut(s) | 182, 484, 490, 560, 1135, 1148 |
| BstV2I | GAAGAC | 1 cut(s) | 717 |
| BstX2I | RGATCY | 1 cut(s) | 940 |
| BstYI | RGATCY | 1 cut(s) | 940 |
| BsuRI | GGCC | 4 cut(s) | 5, 280, 510, 680 |
| BtgI | CCRYGG | 2 cut(s) | 375, 1530 |
| BtsCI | GGATG | 6 cut(s) | 371, 640, 840, 1078, 1210, 1597 |
| BtsI | GCAGTG | 2 cut(s) | 681, 1012 |
| BtsIMutI | CAGTG | 5 cut(s) | 250, 681, 777, 1012, 1567 |
| Cac8I | GCNNGC | 1 cut(s) | 508 |
| CfoI | GCGC | 2 cut(s) | 359, 917 |
| Cfr10I | RCCGGY | 1 cut(s) | 506 |
| Cfr13I | GGNCC | 1 cut(s) | 379 |
| Csp6I | GTAC | 3 cut(s) | 19, 627, 1273 |
| CviAII | CATG | 8 cut(s) | 23, 83, 376, 816, 958, 1013, 1276, 1525 |
| CviQI | GTAC | 3 cut(s) | 19, 627, 1273 |
| DdeI | CTNAG | 4 cut(s) | 150, 405, 797, 1518 |
| DpnI | GATC | 4 cut(s) | 92, 105, 421, 942 |
| DpnII | GATC | 4 cut(s) | 90, 103, 419, 940 |
| EaeI | YGGCCR | 2 cut(s) | 3, 278 |
| Eam1104I | CTCTTC | 2 cut(s) | 950, 1042 |
| EarI | CTCTTC | 2 cut(s) | 950, 1042 |
| Eco130I | CCWWGG | 5 cut(s) | 375, 415, 700, 789, 1099 |
| Eco47I | GGWCC | 1 cut(s) | 379 |
| Eco57I | CTGAAG | 3 cut(s) | 381, 931, 1177 |
| Eco91I | GGTNACC | 1 cut(s) | 325 |
| EcoO65I | GGTNACC | 1 cut(s) | 325 |
| EcoRII | CCWGG | 2 cut(s) | 882, 1200 |
| EcoT14I | CCWWGG | 5 cut(s) | 375, 415, 700, 789, 1099 |
| EcoT22I | ATGCAT | 1 cut(s) | 817 |
| ErhI | CCWWGG | 5 cut(s) | 375, 415, 700, 789, 1099 |
| FaeI | CATG | 8 cut(s) | 26, 86, 379, 819, 961, 1016, 1279, 1528 |
| FaqI | GGGAC | 2 cut(s) | 58, 434 |
| FatI | CATG | 8 cut(s) | 22, 82, 375, 815, 957, 1012, 1275, 1524 |
| FauI | CCCGC | 3 cut(s) | 205, 460, 920 |
| FauNDI | CATATG | 1 cut(s) | 1129 |
| FbaI | TGATCA | 1 cut(s) | 103 |
| FblI | GTMKAC | 1 cut(s) | 771 |
| Fnu4HI | GCNGC | 7 cut(s) | 171, 473, 504, 546, 549, 1137, 1149 |
| FokI | GGATG | 5 cut(s) | 358, 647, 847, 1085, 1217 |
| Fsp4HI | GCNGC | 7 cut(s) | 171, 473, 504, 546, 549, 1137, 1149 |
| FspBI | CTAG | 4 cut(s) | 605, 1041, 1187, 1600 |
| GlaI | GCGC | 2 cut(s) | 358, 916 |
| GluI | GCNGC | 7 cut(s) | 171, 473, 504, 546, 549, 1137, 1149 |
| GsuI | CTGGAG | 2 cut(s) | 708, 1428 |
| HaeII | RGCGCY | 1 cut(s) | 360 |
| HaeIII | GGCC | 4 cut(s) | 5, 280, 510, 680 |
| HapII | CCGG | 1 cut(s) | 507 |
| HhaI | GCGC | 2 cut(s) | 359, 917 |
| Hin1II | CATG | 8 cut(s) | 26, 86, 379, 819, 961, 1016, 1279, 1528 |
| Hin6I | GCGC | 2 cut(s) | 357, 915 |
| HinP1I | GCGC | 2 cut(s) | 357, 915 |
| HincII | GTYRAC | 1 cut(s) | 772 |
| HindII | GTYRAC | 1 cut(s) | 772 |
| HinfI | GANTC | 4 cut(s) | 240, 1045, 1234, 1336 |
| HpaII | CCGG | 1 cut(s) | 507 |
| HphI | GGTGA | 5 cut(s) | 317, 319, 719, 1201, 1394 |
| Hpy166II | GTNNAC | 2 cut(s) | 772, 1530 |
| Hpy188I | TCNGA | 3 cut(s) | 32, 65, 406 |
| Hpy188III | TCNNGA | 7 cut(s) | 754, 1112, 1156, 1245, 1267, 1315, 1504 |
| Hpy8I | GTNNAC | 2 cut(s) | 772, 1530 |
| Hpy99I | CGWCG | 1 cut(s) | 32 |
| HpyAV | CCTTC | 6 cut(s) | 8, 568, 670, 1081, 1375, 1574 |
| HpyCH4III | ACNGT | 3 cut(s) | 427, 448, 1562 |
| HpyF10VI | GCNNNNNNNGC | 4 cut(s) | 500, 554, 1133, 1145 |
| HpyF3I | CTNAG | 4 cut(s) | 150, 405, 797, 1518 |
| Hsp92II | CATG | 8 cut(s) | 26, 86, 379, 819, 961, 1016, 1279, 1528 |
| HspAI | GCGC | 2 cut(s) | 357, 915 |
| KpnI | GGTACC | 1 cut(s) | 22 |
| KroI | GCCGGC | 1 cut(s) | 506 |
| KroNI | GCCGGC | 1 cut(s) | 508 |
| Ksp22I | TGATCA | 1 cut(s) | 103 |
| Kzo9I | GATC | 4 cut(s) | 90, 103, 419, 940 |
| LguI | GCTCTTC | 1 cut(s) | 1042 |
| LmnI | GCTCC | 2 cut(s) | 961, 1090 |
| Lsp1109I | GCAGC | 6 cut(s) | 182, 484, 490, 560, 1135, 1148 |
| LweI | GCATC | 6 cut(s) | 136, 157, 510, 825, 976, 1480 |
| MaeI | CTAG | 4 cut(s) | 605, 1041, 1187, 1600 |
| MaeIII | GTNAC | 3 cut(s) | 305, 325, 1207 |
| MalI | GATC | 4 cut(s) | 92, 105, 421, 942 |
| MboI | GATC | 4 cut(s) | 90, 103, 419, 940 |
| MboII | GAAGA | 8 cut(s) | 374, 722, 937, 995, 1009, 1029, 1330, 1581 |
| MflI | RGATCY | 1 cut(s) | 940 |
| MlsI | TGGCCA | 1 cut(s) | 280 |
| MluCI | AATT | 7 cut(s) | 33, 250, 350, 749, 920, 1240, 1248 |
| MluNI | TGGCCA | 1 cut(s) | 280 |
| MlyI | GAGTC | 2 cut(s) | 1243, 1330 |
| MmeI | TCCRAC | 2 cut(s) | 10, 43 |
| Mox20I | TGGCCA | 1 cut(s) | 280 |
| Mph1103I | ATGCAT | 1 cut(s) | 817 |
| MroNI | GCCGGC | 1 cut(s) | 506 |
| MscI | TGGCCA | 1 cut(s) | 280 |
| MseI | TTAA | 5 cut(s) | 177, 294, 744, 902, 1107 |
| MslI | CAYNNNNRTG | 2 cut(s) | 55, 1280 |
| Msp20I | TGGCCA | 1 cut(s) | 280 |
| MspI | CCGG | 1 cut(s) | 507 |
| MspR9I | CCNGG | 2 cut(s) | 884, 1202 |
| Mva1269I | GAATGC | 2 cut(s) | 439, 1354 |
| MvaI | CCWGG | 2 cut(s) | 884, 1202 |
| MvnI | CGCG | 1 cut(s) | 915 |
| MwoI | GCNNNNNNNGC | 4 cut(s) | 500, 554, 1133, 1145 |
| NaeI | GCCGGC | 1 cut(s) | 508 |
| NcoI | CCATGG | 1 cut(s) | 375 |
| NdeI | CATATG | 1 cut(s) | 1129 |
| NdeII | GATC | 4 cut(s) | 90, 103, 419, 940 |
| NgoMIV | GCCGGC | 1 cut(s) | 506 |
| NlaIII | CATG | 8 cut(s) | 26, 86, 379, 819, 961, 1016, 1279, 1528 |
| NlaIV | GGNNCC | 3 cut(s) | 20, 963, 1290 |
| NmuCI | GTSAC | 3 cut(s) | 305, 325, 1207 |
| NsiI | ATGCAT | 1 cut(s) | 817 |
| NspI | RCATGY | 2 cut(s) | 819, 1528 |
| PciI | ACATGT | 1 cut(s) | 1524 |
| PciSI | GCTCTTC | 1 cut(s) | 1042 |
| PctI | GAATGC | 2 cut(s) | 439, 1354 |
| PdiI | GCCGGC | 1 cut(s) | 508 |
| PfeI | GAWTC | 2 cut(s) | 240, 1045 |
| PfoI | TCCNGGA | 1 cut(s) | 1200 |
| PkrI | GCNGC | 7 cut(s) | 172, 474, 505, 547, 550, 1138, 1150 |
| PleI | GAGTC | 2 cut(s) | 1242, 1330 |
| PpsI | GAGTC | 2 cut(s) | 1242, 1330 |
| PscI | ACATGT | 1 cut(s) | 1524 |
| PshAI | GACNNNNGTC | 1 cut(s) | 1194 |
| PshBI | ATTAAT | 1 cut(s) | 1107 |
| PsiI | TTATAA | 1 cut(s) | 443 |
| Psp6I | CCWGG | 2 cut(s) | 882, 1200 |
| PspEI | GGTNACC | 1 cut(s) | 325 |
| PspGI | CCWGG | 2 cut(s) | 882, 1200 |
| PspN4I | GGNNCC | 3 cut(s) | 20, 963, 1290 |
| PspPI | GGNCC | 1 cut(s) | 379 |
| PsuI | RGATCY | 1 cut(s) | 940 |
| RsaI | GTAC | 3 cut(s) | 20, 628, 1274 |
| RsaNI | GTAC | 3 cut(s) | 19, 627, 1273 |
| RseI | CAYNNNNRTG | 2 cut(s) | 55, 1280 |
| SalI | GTCGAC | 1 cut(s) | 770 |
| SapI | GCTCTTC | 1 cut(s) | 1042 |
| SaqAI | TTAA | 5 cut(s) | 177, 294, 744, 902, 1107 |
| SatI | GCNGC | 7 cut(s) | 171, 473, 504, 546, 549, 1137, 1149 |
| Sau3AI | GATC | 4 cut(s) | 90, 103, 419, 940 |
| Sau96I | GGNCC | 1 cut(s) | 379 |
| SchI | GAGTC | 2 cut(s) | 1243, 1330 |
| ScrFI | CCNGG | 2 cut(s) | 884, 1202 |
| SfaNI | GCATC | 6 cut(s) | 136, 157, 510, 825, 976, 1480 |
| SinI | GGWCC | 1 cut(s) | 379 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 55, 1280 |
| SmlI | CTYRAG | 1 cut(s) | 1504 |
| SmoI | CTYRAG | 1 cut(s) | 1504 |
| Sse9I | AATT | 7 cut(s) | 33, 250, 350, 749, 920, 1240, 1248 |
| SsiI | CCGC | 4 cut(s) | 198, 453, 546, 913 |
| SspMI | CTAG | 4 cut(s) | 605, 1041, 1187, 1600 |
| StyD4I | CCNGG | 2 cut(s) | 882, 1200 |
| StyI | CCWWGG | 5 cut(s) | 375, 415, 700, 789, 1099 |
| TaaI | ACNGT | 3 cut(s) | 427, 448, 1562 |
| TaqI | TCGA | 3 cut(s) | 612, 771, 924 |
| TasI | AATT | 7 cut(s) | 33, 250, 350, 749, 920, 1240, 1248 |
| TatI | WGTACW | 1 cut(s) | 1272 |
| TauI | GCSGC | 1 cut(s) | 548 |
| TfiI | GAWTC | 2 cut(s) | 240, 1045 |
| Tru1I | TTAA | 5 cut(s) | 177, 294, 744, 902, 1107 |
| Tru9I | TTAA | 5 cut(s) | 177, 294, 744, 902, 1107 |
| TscAI | CASTG | 5 cut(s) | 250, 688, 784, 1012, 1567 |
| TseFI | GTSAC | 3 cut(s) | 305, 325, 1207 |
| TseI | GCWGC | 6 cut(s) | 170, 472, 503, 548, 1136, 1148 |
| Tsp45I | GTSAC | 3 cut(s) | 305, 325, 1207 |
| TspDTI | ATGAA | 3 cut(s) | 549, 1010, 1292 |
| TspRI | CASTG | 5 cut(s) | 250, 688, 784, 1012, 1567 |
| VpaK11BI | GGWCC | 1 cut(s) | 379 |
| VspI | ATTAAT | 1 cut(s) | 1107 |
| XapI | RAATTY | 1 cut(s) | 920 |
| XceI | RCATGY | 2 cut(s) | 819, 1528 |
| XmiI | GTMKAC | 1 cut(s) | 771 |
| XspI | CTAG | 4 cut(s) | 605, 1041, 1187, 1600 |
| Zsp2I | ATGCAT | 1 cut(s) | 817 |