Belongs to the heat shock protein 70 family
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 342 |
| AccB1I | GGYRCC | 2 cut(s) | 587, 1142 |
| AccII | CGCG | 1 cut(s) | 1516 |
| AciI | CCGC | 5 cut(s) | 17, 183, 366, 1328, 1516 |
| AclI | AACGTT | 1 cut(s) | 609 |
| AclWI | GGATC | 4 cut(s) | 436, 957, 1051, 1626 |
| AcoI | YGGCCR | 1 cut(s) | 1429 |
| AcsI | RAATTY | 3 cut(s) | 929, 1053, 1705 |
| AfaI | GTAC | 5 cut(s) | 637, 753, 1065, 1358, 1607 |
| AfeI | AGCGCT | 1 cut(s) | 236 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 41 |
| AflII | CTTAAG | 1 cut(s) | 656 |
| AjnI | CCWGG | 1 cut(s) | 1426 |
| AleI | CACNNNNGTG | 1 cut(s) | 93 |
| AloI | GAACNNNNNNTCC | 2 cut(s) | 594, 626 |
| AluBI | AGCT | 9 cut(s) | 554, 689, 695, 1072, 1102, 1157, 1379, 1411, 1651 |
| AluI | AGCT | 9 cut(s) | 554, 689, 695, 1072, 1102, 1157, 1379, 1411, 1651 |
| Alw21I | GWGCWC | 1 cut(s) | 1413 |
| Alw26I | GTCTC | 1 cut(s) | 1498 |
| AlwI | GGATC | 4 cut(s) | 436, 957, 1051, 1626 |
| Aor51HI | AGCGCT | 1 cut(s) | 236 |
| AoxI | GGCC | 4 cut(s) | 290, 417, 1429, 1850 |
| ApeKI | GCWGC | 2 cut(s) | 554, 557 |
| ApoI | RAATTY | 3 cut(s) | 929, 1053, 1705 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 872, 904 |
| Asp700I | GAANNNNTTC | 2 cut(s) | 209, 847 |
| AspLEI | GCGC | 1 cut(s) | 237 |
| AspS9I | GGNCC | 1 cut(s) | 32 |
| AsuHPI | GGTGA | 9 cut(s) | 188, 694, 710, 728, 751, 1184, 1376, 1444, 1531 |
| AsuII | TTCGAA | 2 cut(s) | 763, 891 |
| AvaII | GGWCC | 1 cut(s) | 32 |
| BalI | TGGCCA | 1 cut(s) | 1431 |
| BanI | GGYRCC | 2 cut(s) | 587, 1142 |
| BanII | GRGCYC | 1 cut(s) | 1413 |
| Bbv12I | GWGCWC | 1 cut(s) | 1413 |
| BbvCI | CCTCAGC | 1 cut(s) | 369 |
| BbvI | GCAGC | 2 cut(s) | 541, 569 |
| BccI | CCATC | 5 cut(s) | 1085, 1244, 1295, 1675, 1755 |
| BciT130I | CCWGG | 1 cut(s) | 1428 |
| BclI | TGATCA | 2 cut(s) | 115, 333 |
| BcoDI | GTCTC | 1 cut(s) | 1498 |
| BfaI | CTAG | 1 cut(s) | 1864 |
| BfmI | CTRYAG | 2 cut(s) | 555, 690 |
| BfoI | RGCGCY | 1 cut(s) | 238 |
| BfrI | CTTAAG | 1 cut(s) | 656 |
| BisI | GCNGC | 5 cut(s) | 18, 183, 366, 555, 558 |
| BlpI | GCTNAGC | 1 cut(s) | 1647 |
| BlsI | GCNGC | 5 cut(s) | 19, 184, 367, 556, 559 |
| Bme1390I | CCNGG | 1 cut(s) | 1428 |
| Bme18I | GGWCC | 1 cut(s) | 32 |
| BmgT120I | GGNCC | 1 cut(s) | 32 |
| BmiI | GGNNCC | 4 cut(s) | 589, 972, 1144, 1526 |
| BmrFI | CCNGG | 1 cut(s) | 1428 |
| BmrI | ACTGGG | 1 cut(s) | 171 |
| BmsI | GCATC | 9 cut(s) | 169, 217, 390, 490, 985, 1428, 1462, 1679, 1729 |
| BmuI | ACTGGG | 1 cut(s) | 171 |
| BoxI | GACNNNNGTC | 1 cut(s) | 1319 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 866, 898 |
| Bpu10I | CCTNAGC | 1 cut(s) | 369 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 1647 |
| Bpu14I | TTCGAA | 2 cut(s) | 763, 891 |
| Bsa29I | ATCGAT | 1 cut(s) | 48 |
| BsaBI | GATNNNNATC | 3 cut(s) | 44, 332, 705 |
| BsaJI | CCNNGG | 3 cut(s) | 1059, 1231, 1500 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 41 |
| Bse118I | RCCGGY | 1 cut(s) | 503 |
| Bse1I | ACTGG | 1 cut(s) | 166 |
| Bse3DI | GCAATG | 2 cut(s) | 390, 561 |
| Bse8I | GATNNNNATC | 3 cut(s) | 44, 332, 705 |
| BseBI | CCWGG | 1 cut(s) | 1428 |
| BseCI | ATCGAT | 1 cut(s) | 48 |
| BseDI | CCNNGG | 3 cut(s) | 1059, 1231, 1500 |
| BseGI | GGATG | 4 cut(s) | 505, 1219, 1287, 1419 |
| BseJI | GATNNNNATC | 3 cut(s) | 44, 332, 705 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 41 |
| BseMI | GCAATG | 2 cut(s) | 390, 561 |
| BseMII | CTCAG | 1 cut(s) | 360 |
| BseNI | ACTGG | 1 cut(s) | 166 |
| BseRI | GAGGAG | 2 cut(s) | 386, 1537 |
| BseXI | GCAGC | 2 cut(s) | 541, 569 |
| BseYI | CCCAGC | 1 cut(s) | 460 |
| BsgI | GTGCAG | 1 cut(s) | 1257 |
| Bsh1236I | CGCG | 1 cut(s) | 1516 |
| Bsh1285I | CGRYCG | 1 cut(s) | 42 |
| BshFI | GGCC | 4 cut(s) | 292, 419, 1431, 1852 |
| BshNI | GGYRCC | 2 cut(s) | 587, 1142 |
| BshVI | ATCGAT | 1 cut(s) | 48 |
| BsiEI | CGRYCG | 1 cut(s) | 42 |
| BsiHKAI | GWGCWC | 1 cut(s) | 1413 |
| BsiSI | CCGG | 1 cut(s) | 504 |
| BslFI | GGGAC | 4 cut(s) | 329, 443, 1256, 1344 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 41 |
| BsmAI | GTCTC | 1 cut(s) | 1498 |
| BsmFI | GGGAC | 4 cut(s) | 329, 443, 1256, 1344 |
| BsnI | GGCC | 4 cut(s) | 292, 419, 1431, 1852 |
| Bsp119I | TTCGAA | 2 cut(s) | 763, 891 |
| Bsp1286I | GDGCHC | 1 cut(s) | 1413 |
| Bsp1407I | TGTACA | 1 cut(s) | 751 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 1647 |
| BspACI | CCGC | 5 cut(s) | 17, 183, 366, 1328, 1516 |
| BspANI | GGCC | 4 cut(s) | 292, 419, 1431, 1852 |
| BspCNI | CTCAG | 1 cut(s) | 361 |
| BspDI | ATCGAT | 1 cut(s) | 48 |
| BspFNI | CGCG | 1 cut(s) | 1516 |
| BspLI | GGNNCC | 4 cut(s) | 589, 972, 1144, 1526 |
| BspMAI | CTGCAG | 1 cut(s) | 559 |
| BspPI | GGATC | 4 cut(s) | 436, 957, 1051, 1626 |
| BspT104I | TTCGAA | 2 cut(s) | 763, 891 |
| BspT107I | GGYRCC | 2 cut(s) | 587, 1142 |
| BspTI | CTTAAG | 1 cut(s) | 656 |
| BsrDI | GCAATG | 2 cut(s) | 390, 561 |
| BsrFI | RCCGGY | 1 cut(s) | 503 |
| BsrGI | TGTACA | 1 cut(s) | 751 |
| BsrI | ACTGG | 1 cut(s) | 166 |
| BssAI | RCCGGY | 1 cut(s) | 503 |
| BssECI | CCNNGG | 3 cut(s) | 1059, 1231, 1500 |
| BssT1I | CCWWGG | 3 cut(s) | 1059, 1231, 1500 |
| Bst2UI | CCWGG | 1 cut(s) | 1428 |
| Bst4CI | ACNGT | 9 cut(s) | 138, 220, 436, 457, 572, 1172, 1481, 1690, 1753 |
| Bst6I | CTCTTC | 3 cut(s) | 660, 930, 1514 |
| BstAFI | CTTAAG | 1 cut(s) | 656 |
| BstAPI | GCANNNNNTGC | 2 cut(s) | 563, 1142 |
| BstAUI | TGTACA | 1 cut(s) | 751 |
| BstBI | TTCGAA | 2 cut(s) | 763, 891 |
| BstC8I | GCNNGC | 2 cut(s) | 1559, 1653 |
| BstDEI | CTNAG | 5 cut(s) | 369, 806, 1296, 1647, 1854 |
| BstEII | GGTNACC | 1 cut(s) | 1450 |
| BstF5I | GGATG | 4 cut(s) | 505, 1219, 1287, 1419 |
| BstFNI | CGCG | 1 cut(s) | 1516 |
| BstH2I | RGCGCY | 1 cut(s) | 238 |
| BstHHI | GCGC | 1 cut(s) | 237 |
| BstMAI | GTCTC | 1 cut(s) | 1498 |
| BstMCI | CGRYCG | 1 cut(s) | 42 |
| BstMWI | GCNNNNNNNGC | 5 cut(s) | 17, 91, 563, 1142, 1652 |
| BstNI | CCWGG | 1 cut(s) | 1428 |
| BstPAI | GACNNNNGTC | 1 cut(s) | 1319 |
| BstPI | GGTNACC | 1 cut(s) | 1450 |
| BstSCI | CCNGG | 1 cut(s) | 1426 |
| BstSFI | CTRYAG | 2 cut(s) | 555, 690 |
| BstUI | CGCG | 1 cut(s) | 1516 |
| BstV1I | GCAGC | 2 cut(s) | 541, 569 |
| BstX2I | RGATCY | 2 cut(s) | 949, 1043 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 967 |
| BstYI | RGATCY | 2 cut(s) | 949, 1043 |
| Bsu15I | ATCGAT | 1 cut(s) | 48 |
| BsuRI | GGCC | 4 cut(s) | 292, 419, 1431, 1852 |
| BsuTUI | ATCGAT | 1 cut(s) | 48 |
| BtgZI | GCGATG | 1 cut(s) | 1774 |
| BtsCI | GGATG | 4 cut(s) | 505, 1219, 1287, 1419 |
| BtsIMutI | CAGTG | 3 cut(s) | 159, 216, 453 |
| Cac8I | GCNNGC | 2 cut(s) | 1559, 1653 |
| CfoI | GCGC | 1 cut(s) | 237 |
| Cfr10I | RCCGGY | 1 cut(s) | 503 |
| Cfr13I | GGNCC | 1 cut(s) | 32 |
| ClaI | ATCGAT | 1 cut(s) | 48 |
| Csp6I | GTAC | 5 cut(s) | 636, 752, 1064, 1357, 1606 |
| CspCI | CAANNNNNGTGG | 1 cut(s) | 29 |
| CviAII | CATG | 4 cut(s) | 69, 280, 1342, 1846 |
| CviQI | GTAC | 5 cut(s) | 636, 752, 1064, 1357, 1606 |
| DdeI | CTNAG | 5 cut(s) | 369, 806, 1296, 1647, 1854 |
| DraI | TTTAAA | 1 cut(s) | 1351 |
| EaeI | YGGCCR | 1 cut(s) | 1429 |
| Eam1104I | CTCTTC | 3 cut(s) | 660, 930, 1514 |
| EarI | CTCTTC | 3 cut(s) | 660, 930, 1514 |
| Ecl136II | GAGCTC | 1 cut(s) | 1411 |
| Eco130I | CCWWGG | 3 cut(s) | 1059, 1231, 1500 |
| Eco147I | AGGCCT | 1 cut(s) | 419 |
| Eco24I | GRGCYC | 1 cut(s) | 1413 |
| Eco32I | GATATC | 1 cut(s) | 378 |
| Eco47I | GGWCC | 1 cut(s) | 32 |
| Eco47III | AGCGCT | 1 cut(s) | 236 |
| Eco53kI | GAGCTC | 1 cut(s) | 1411 |
| Eco91I | GGTNACC | 1 cut(s) | 1450 |
| EcoICRI | GAGCTC | 1 cut(s) | 1411 |
| EcoO65I | GGTNACC | 1 cut(s) | 1450 |
| EcoRI | GAATTC | 1 cut(s) | 1053 |
| EcoRII | CCWGG | 1 cut(s) | 1426 |
| EcoRV | GATATC | 1 cut(s) | 378 |
| EcoT14I | CCWWGG | 3 cut(s) | 1059, 1231, 1500 |
| EcoT22I | ATGCAT | 2 cut(s) | 1343, 1672 |
| EcoT38I | GRGCYC | 1 cut(s) | 1413 |
| ErhI | CCWWGG | 3 cut(s) | 1059, 1231, 1500 |
| FaeI | CATG | 4 cut(s) | 72, 283, 1345, 1849 |
| FaqI | GGGAC | 4 cut(s) | 329, 443, 1256, 1344 |
| FatI | CATG | 4 cut(s) | 68, 279, 1341, 1845 |
| FauI | CCCGC | 1 cut(s) | 1321 |
| FauNDI | CATATG | 2 cut(s) | 142, 1138 |
| FbaI | TGATCA | 2 cut(s) | 115, 333 |
| Fnu4HI | GCNGC | 5 cut(s) | 18, 183, 366, 555, 558 |
| FokI | GGATG | 4 cut(s) | 512, 1226, 1274, 1406 |
| FriOI | GRGCYC | 1 cut(s) | 1413 |
| Fsp4HI | GCNGC | 5 cut(s) | 18, 183, 366, 555, 558 |
| FspBI | CTAG | 1 cut(s) | 1864 |
| GlaI | GCGC | 1 cut(s) | 236 |
| GluI | GCNGC | 5 cut(s) | 18, 183, 366, 555, 558 |
| GsaI | CCCAGC | 1 cut(s) | 464 |
| HaeII | RGCGCY | 1 cut(s) | 238 |
| HaeIII | GGCC | 4 cut(s) | 292, 419, 1431, 1852 |
| HapII | CCGG | 1 cut(s) | 504 |
| HhaI | GCGC | 1 cut(s) | 237 |
| Hin1II | CATG | 4 cut(s) | 72, 283, 1345, 1849 |
| Hin6I | GCGC | 1 cut(s) | 235 |
| HinP1I | GCGC | 1 cut(s) | 235 |
| HindIII | AAGCTT | 1 cut(s) | 1649 |
| HinfI | GANTC | 3 cut(s) | 45, 309, 881 |
| HpaII | CCGG | 1 cut(s) | 504 |
| HphI | GGTGA | 9 cut(s) | 188, 694, 710, 728, 751, 1184, 1376, 1444, 1531 |
| Hpy166II | GTNNAC | 3 cut(s) | 742, 1357, 1720 |
| Hpy188I | TCNGA | 2 cut(s) | 44, 428 |
| Hpy188III | TCNNGA | 5 cut(s) | 575, 1121, 1186, 1196, 1622 |
| Hpy8I | GTNNAC | 3 cut(s) | 742, 1357, 1720 |
| HpyAV | CCTTC | 7 cut(s) | 23, 220, 578, 662, 887, 1183, 1255 |
| HpyCH4III | ACNGT | 9 cut(s) | 138, 220, 436, 457, 572, 1172, 1481, 1690, 1753 |
| HpyCH4IV | ACGT | 2 cut(s) | 484, 609 |
| HpyF10VI | GCNNNNNNNGC | 5 cut(s) | 17, 91, 563, 1142, 1652 |
| HpyF3I | CTNAG | 5 cut(s) | 369, 806, 1296, 1647, 1854 |
| HpySE526I | ACGT | 2 cut(s) | 484, 609 |
| Hsp92II | CATG | 4 cut(s) | 72, 283, 1345, 1849 |
| HspAI | GCGC | 1 cut(s) | 235 |
| Ksp22I | TGATCA | 2 cut(s) | 115, 333 |
| LmnI | GCTCC | 3 cut(s) | 970, 1099, 1524 |
| Lsp1109I | GCAGC | 2 cut(s) | 541, 569 |
| LweI | GCATC | 9 cut(s) | 169, 217, 390, 490, 985, 1428, 1462, 1679, 1729 |
| MaeI | CTAG | 1 cut(s) | 1864 |
| MaeII | ACGT | 2 cut(s) | 484, 609 |
| MaeIII | GTNAC | 3 cut(s) | 1216, 1312, 1450 |
| MflI | RGATCY | 2 cut(s) | 949, 1043 |
| MhlI | GDGCHC | 1 cut(s) | 1413 |
| MlsI | TGGCCA | 1 cut(s) | 1431 |
| MluNI | TGGCCA | 1 cut(s) | 1431 |
| MlyI | GAGTC | 2 cut(s) | 318, 875 |
| MmeI | TCCRAC | 1 cut(s) | 1191 |
| Mox20I | TGGCCA | 1 cut(s) | 1431 |
| Mph1103I | ATGCAT | 2 cut(s) | 1343, 1672 |
| MroXI | GAANNNNTTC | 2 cut(s) | 209, 847 |
| MscI | TGGCCA | 1 cut(s) | 1431 |
| MseI | TTAA | 4 cut(s) | 170, 471, 657, 1350 |
| MslI | CAYNNNNRTG | 1 cut(s) | 93 |
| Msp20I | TGGCCA | 1 cut(s) | 1431 |
| MspA1I | CMGCKG | 2 cut(s) | 368, 554 |
| MspCI | CTTAAG | 1 cut(s) | 656 |
| MspI | CCGG | 1 cut(s) | 504 |
| MspR9I | CCNGG | 1 cut(s) | 1428 |
| MvaI | CCWGG | 1 cut(s) | 1428 |
| MvnI | CGCG | 1 cut(s) | 1516 |
| MwoI | GCNNNNNNNGC | 5 cut(s) | 17, 91, 563, 1142, 1652 |
| NdeI | CATATG | 2 cut(s) | 142, 1138 |
| NlaIII | CATG | 4 cut(s) | 72, 283, 1345, 1849 |
| NlaIV | GGNNCC | 4 cut(s) | 589, 972, 1144, 1526 |
| NmuCI | GTSAC | 3 cut(s) | 1216, 1312, 1450 |
| NsiI | ATGCAT | 2 cut(s) | 1343, 1672 |
| NspV | TTCGAA | 2 cut(s) | 763, 891 |
| OliI | CACNNNNGTG | 1 cut(s) | 93 |
| PceI | AGGCCT | 1 cut(s) | 419 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 917 |
| PdmI | GAANNNNTTC | 2 cut(s) | 209, 847 |
| PfeI | GAWTC | 1 cut(s) | 45 |
| PkrI | GCNGC | 5 cut(s) | 19, 184, 367, 556, 559 |
| Ple19I | CGATCG | 1 cut(s) | 42 |
| PleI | GAGTC | 2 cut(s) | 317, 875 |
| PpsI | GAGTC | 2 cut(s) | 317, 875 |
| PshAI | GACNNNNGTC | 1 cut(s) | 1319 |
| PsiI | TTATAA | 1 cut(s) | 342 |
| Psp124BI | GAGCTC | 1 cut(s) | 1413 |
| Psp1406I | AACGTT | 1 cut(s) | 609 |
| Psp6I | CCWGG | 1 cut(s) | 1426 |
| PspEI | GGTNACC | 1 cut(s) | 1450 |
| PspFI | CCCAGC | 1 cut(s) | 460 |
| PspGI | CCWGG | 1 cut(s) | 1426 |
| PspN4I | GGNNCC | 4 cut(s) | 589, 972, 1144, 1526 |
| PspPI | GGNCC | 1 cut(s) | 32 |
| PstI | CTGCAG | 1 cut(s) | 559 |
| PsuI | RGATCY | 2 cut(s) | 949, 1043 |
| PvuI | CGATCG | 1 cut(s) | 42 |
| PvuII | CAGCTG | 1 cut(s) | 554 |
| RsaI | GTAC | 5 cut(s) | 637, 753, 1065, 1358, 1607 |
| RsaNI | GTAC | 5 cut(s) | 636, 752, 1064, 1357, 1606 |
| RseI | CAYNNNNRTG | 1 cut(s) | 93 |
| SacI | GAGCTC | 1 cut(s) | 1413 |
| SaqAI | TTAA | 4 cut(s) | 170, 471, 657, 1350 |
| SatI | GCNGC | 5 cut(s) | 18, 183, 366, 555, 558 |
| Sau96I | GGNCC | 1 cut(s) | 32 |
| SchI | GAGTC | 2 cut(s) | 318, 875 |
| ScrFI | CCNGG | 1 cut(s) | 1428 |
| SduI | GDGCHC | 1 cut(s) | 1413 |
| SfaNI | GCATC | 9 cut(s) | 169, 217, 390, 490, 985, 1428, 1462, 1679, 1729 |
| SfcI | CTRYAG | 2 cut(s) | 555, 690 |
| SfuI | TTCGAA | 2 cut(s) | 763, 891 |
| SinI | GGWCC | 1 cut(s) | 32 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 93 |
| SmlI | CTYRAG | 1 cut(s) | 656 |
| SmoI | CTYRAG | 1 cut(s) | 656 |
| SseBI | AGGCCT | 1 cut(s) | 419 |
| SsiI | CCGC | 5 cut(s) | 17, 183, 366, 1328, 1516 |
| SspI | AATATT | 1 cut(s) | 595 |
| SspMI | CTAG | 1 cut(s) | 1864 |
| SstI | GAGCTC | 1 cut(s) | 1413 |
| StuI | AGGCCT | 1 cut(s) | 419 |
| StyD4I | CCNGG | 1 cut(s) | 1426 |
| StyI | CCWWGG | 3 cut(s) | 1059, 1231, 1500 |
| TaaI | ACNGT | 9 cut(s) | 138, 220, 436, 457, 572, 1172, 1481, 1690, 1753 |
| TaiI | ACGT | 2 cut(s) | 487, 612 |
| TaqI | TCGA | 6 cut(s) | 48, 763, 891, 911, 1185, 1323 |
| TatI | WGTACW | 1 cut(s) | 751 |
| TauI | GCSGC | 3 cut(s) | 20, 185, 368 |
| TfiI | GAWTC | 1 cut(s) | 45 |
| Tru1I | TTAA | 4 cut(s) | 170, 471, 657, 1350 |
| Tru9I | TTAA | 4 cut(s) | 170, 471, 657, 1350 |
| TscAI | CASTG | 3 cut(s) | 166, 223, 460 |
| TseFI | GTSAC | 3 cut(s) | 1216, 1312, 1450 |
| TseI | GCWGC | 2 cut(s) | 554, 557 |
| Tsp45I | GTSAC | 3 cut(s) | 1216, 1312, 1450 |
| TspDTI | ATGAA | 5 cut(s) | 296, 363, 536, 1140, 1274 |
| TspGWI | ACGGA | 1 cut(s) | 1412 |
| TspRI | CASTG | 3 cut(s) | 166, 223, 460 |
| Vha464I | CTTAAG | 1 cut(s) | 656 |
| VpaK11BI | GGWCC | 1 cut(s) | 32 |
| XapI | RAATTY | 3 cut(s) | 929, 1053, 1705 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 1483 |
| XmnI | GAANNNNTTC | 2 cut(s) | 209, 847 |
| XspI | CTAG | 1 cut(s) | 1864 |
| Zsp2I | ATGCAT | 2 cut(s) | 1343, 1672 |