Belongs to the heat shock protein 70 family
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 1304 |
| AciI | CCGC | 7 cut(s) | 6, 394, 495, 579, 621, 1016, 1127 |
| AcoI | YGGCCR | 1 cut(s) | 278 |
| AcsI | RAATTY | 3 cut(s) | 749, 1032, 1546 |
| AfaI | GTAC | 1 cut(s) | 628 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 1330 |
| AloI | GAACNNNNNNTCC | 2 cut(s) | 1113, 1145 |
| AluBI | AGCT | 8 cut(s) | 154, 551, 686, 735, 794, 1081, 1124, 1136 |
| AluI | AGCT | 8 cut(s) | 154, 551, 686, 735, 794, 1081, 1124, 1136 |
| Alw21I | GWGCWC | 2 cut(s) | 78, 796 |
| Alw26I | GTCTC | 3 cut(s) | 684, 829, 1489 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 503 |
| Ama87I | CYCGRG | 1 cut(s) | 1208 |
| AoxI | GGCC | 4 cut(s) | 278, 408, 508, 1150 |
| ApeKI | GCWGC | 6 cut(s) | 170, 356, 545, 548, 1124, 1136 |
| ApoI | RAATTY | 3 cut(s) | 749, 1032, 1546 |
| AspS9I | GGNCC | 2 cut(s) | 299, 949 |
| AsuHPI | GGTGA | 8 cut(s) | 414, 436, 668, 689, 719, 742, 1186, 1367 |
| AsuII | TTCGAA | 1 cut(s) | 669 |
| AvaI | CYCGRG | 1 cut(s) | 1208 |
| AvaII | GGWCC | 2 cut(s) | 299, 949 |
| BalI | TGGCCA | 1 cut(s) | 280 |
| BanII | GRGCYC | 1 cut(s) | 796 |
| BauI | CACGAG | 1 cut(s) | 77 |
| BbsI | GAAGAC | 1 cut(s) | 1057 |
| Bbv12I | GWGCWC | 2 cut(s) | 78, 796 |
| BbvCI | CCTCAGC | 1 cut(s) | 405 |
| BbvI | GCAGC | 6 cut(s) | 157, 343, 557, 560, 1111, 1123 |
| BccI | CCATC | 3 cut(s) | 133, 653, 1161 |
| BcgI | CGANNNNNNTGC | 4 cut(s) | 524, 558, 1553, 1587 |
| BciVI | GTATCC | 1 cut(s) | 1484 |
| BclI | TGATCA | 1 cut(s) | 103 |
| BcoDI | GTCTC | 3 cut(s) | 684, 829, 1489 |
| BfaI | CTAG | 6 cut(s) | 632, 770, 900, 1029, 1275, 1585 |
| BfmI | CTRYAG | 3 cut(s) | 543, 681, 1137 |
| BfuI | GTATCC | 1 cut(s) | 1484 |
| BglII | AGATCT | 1 cut(s) | 712 |
| BisI | GCNGC | 8 cut(s) | 171, 357, 495, 546, 549, 580, 1125, 1137 |
| BlsI | GCNGC | 8 cut(s) | 172, 358, 496, 547, 550, 581, 1126, 1138 |
| Bme18I | GGWCC | 2 cut(s) | 299, 949 |
| BmeT110I | CYCGRG | 1 cut(s) | 1208 |
| BmgT120I | GGNCC | 2 cut(s) | 299, 949 |
| BmiI | GGNNCC | 2 cut(s) | 951, 1422 |
| BmrI | ACTGGG | 1 cut(s) | 1515 |
| BmsI | GCATC | 7 cut(s) | 205, 238, 381, 481, 510, 964, 1453 |
| BmuI | ACTGGG | 1 cut(s) | 1515 |
| BpiI | GAAGAC | 1 cut(s) | 1057 |
| BpmI | CTGGAG | 1 cut(s) | 708 |
| Bpu10I | CCTNAGC | 1 cut(s) | 405 |
| Bpu14I | TTCGAA | 1 cut(s) | 669 |
| BpuEI | CTTGAG | 1 cut(s) | 1259 |
| BsaBI | GATNNNNATC | 2 cut(s) | 524, 1526 |
| BsaJI | CCNNGG | 1 cut(s) | 700 |
| BsaXI | ACNNNNNCTCC | 4 cut(s) | 229, 259, 1113, 1143 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 1330 |
| Bse1I | ACTGG | 2 cut(s) | 784, 1510 |
| Bse3DI | GCAATG | 2 cut(s) | 641, 1000 |
| Bse8I | GATNNNNATC | 2 cut(s) | 524, 1526 |
| BseDI | CCNNGG | 1 cut(s) | 700 |
| BseGI | GGATG | 1 cut(s) | 1195 |
| BseJI | GATNNNNATC | 2 cut(s) | 524, 1526 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 1330 |
| BseMI | GCAATG | 2 cut(s) | 641, 1000 |
| BseMII | CTCAG | 4 cut(s) | 267, 297, 396, 1500 |
| BseNI | ACTGG | 2 cut(s) | 784, 1510 |
| BseRI | GAGGAG | 3 cut(s) | 24, 27, 847 |
| BseXI | GCAGC | 6 cut(s) | 157, 343, 557, 560, 1111, 1123 |
| Bsh1285I | CGRYCG | 1 cut(s) | 1335 |
| BshFI | GGCC | 4 cut(s) | 280, 410, 510, 1152 |
| BsiEI | CGRYCG | 1 cut(s) | 1335 |
| BsiHKAI | GWGCWC | 2 cut(s) | 78, 796 |
| BsiHKCI | CYCGRG | 1 cut(s) | 1208 |
| BslFI | GGGAC | 2 cut(s) | 58, 434 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 1330 |
| BsmAI | GTCTC | 3 cut(s) | 684, 829, 1489 |
| BsmFI | GGGAC | 2 cut(s) | 58, 434 |
| BsnI | GGCC | 4 cut(s) | 280, 410, 510, 1152 |
| BsoBI | CYCGRG | 1 cut(s) | 1208 |
| Bsp119I | TTCGAA | 1 cut(s) | 669 |
| Bsp1286I | GDGCHC | 2 cut(s) | 78, 796 |
| Bsp143I | GATC | 5 cut(s) | 103, 183, 712, 1159, 1527 |
| BspACI | CCGC | 7 cut(s) | 6, 394, 495, 579, 621, 1016, 1127 |
| BspANI | GGCC | 4 cut(s) | 280, 410, 510, 1152 |
| BspCNI | CTCAG | 4 cut(s) | 266, 298, 397, 1499 |
| BspLI | GGNNCC | 2 cut(s) | 951, 1422 |
| BspMAI | CTGCAG | 2 cut(s) | 547, 1141 |
| BspQI | GCTCTTC | 2 cut(s) | 1030, 1286 |
| BspT104I | TTCGAA | 1 cut(s) | 669 |
| BsrDI | GCAATG | 2 cut(s) | 641, 1000 |
| BsrI | ACTGG | 2 cut(s) | 784, 1510 |
| BssECI | CCNNGG | 1 cut(s) | 700 |
| BssMI | GATC | 5 cut(s) | 103, 183, 712, 1159, 1527 |
| BssSI | CACGAG | 1 cut(s) | 77 |
| BssT1I | CCWWGG | 1 cut(s) | 700 |
| Bst2BI | CACGAG | 1 cut(s) | 77 |
| Bst4CI | ACNGT | 2 cut(s) | 448, 1535 |
| Bst6I | CTCTTC | 3 cut(s) | 336, 1030, 1286 |
| BstAPI | GCANNNNNTGC | 2 cut(s) | 503, 554 |
| BstBI | TTCGAA | 1 cut(s) | 669 |
| BstC8I | GCNNGC | 1 cut(s) | 508 |
| BstDEI | CTNAG | 6 cut(s) | 253, 306, 405, 797, 1437, 1486 |
| BstF5I | GGATG | 1 cut(s) | 1195 |
| BstKTI | GATC | 5 cut(s) | 106, 186, 715, 1162, 1530 |
| BstMAI | GTCTC | 3 cut(s) | 684, 829, 1489 |
| BstMBI | GATC | 5 cut(s) | 103, 183, 712, 1159, 1527 |
| BstMCI | CGRYCG | 1 cut(s) | 1335 |
| BstMWI | GCNNNNNNNGC | 6 cut(s) | 503, 554, 588, 1121, 1133, 1398 |
| BstNSI | RCATGY | 2 cut(s) | 86, 819 |
| BstSFI | CTRYAG | 3 cut(s) | 543, 681, 1137 |
| BstV1I | GCAGC | 6 cut(s) | 157, 343, 557, 560, 1111, 1123 |
| BstV2I | GAAGAC | 1 cut(s) | 1057 |
| BstX2I | RGATCY | 1 cut(s) | 712 |
| BstYI | RGATCY | 1 cut(s) | 712 |
| BsuI | GTATCC | 1 cut(s) | 1484 |
| BsuRI | GGCC | 4 cut(s) | 280, 410, 510, 1152 |
| BtgZI | GCGATG | 1 cut(s) | 987 |
| BtsCI | GGATG | 1 cut(s) | 1195 |
| BtsI | GCAGTG | 2 cut(s) | 72, 501 |
| BtsIMutI | CAGTG | 4 cut(s) | 72, 501, 777, 1540 |
| Cac8I | GCNNGC | 1 cut(s) | 508 |
| CaiI | CAGNNNCTG | 1 cut(s) | 503 |
| Cfr13I | GGNCC | 2 cut(s) | 299, 949 |
| CseI | GACGC | 2 cut(s) | 176, 464 |
| Csp6I | GTAC | 1 cut(s) | 627 |
| CviAII | CATG | 9 cut(s) | 23, 57, 83, 316, 816, 946, 1001, 1249, 1498 |
| CviQI | GTAC | 1 cut(s) | 627 |
| DdeI | CTNAG | 6 cut(s) | 253, 306, 405, 797, 1437, 1486 |
| DpnI | GATC | 5 cut(s) | 105, 185, 714, 1161, 1529 |
| DpnII | GATC | 5 cut(s) | 103, 183, 712, 1159, 1527 |
| EaeI | YGGCCR | 1 cut(s) | 278 |
| Eam1104I | CTCTTC | 3 cut(s) | 336, 1030, 1286 |
| EarI | CTCTTC | 3 cut(s) | 336, 1030, 1286 |
| EciI | GGCGGA | 1 cut(s) | 21 |
| Ecl136II | GAGCTC | 1 cut(s) | 794 |
| Eco130I | CCWWGG | 1 cut(s) | 700 |
| Eco147I | AGGCCT | 1 cut(s) | 410 |
| Eco24I | GRGCYC | 1 cut(s) | 796 |
| Eco47I | GGWCC | 2 cut(s) | 299, 949 |
| Eco53kI | GAGCTC | 1 cut(s) | 794 |
| Eco88I | CYCGRG | 1 cut(s) | 1208 |
| EcoICRI | GAGCTC | 1 cut(s) | 794 |
| EcoO109I | RGGNCCY | 1 cut(s) | 299 |
| EcoRI | GAATTC | 2 cut(s) | 749, 1032 |
| EcoT14I | CCWWGG | 1 cut(s) | 700 |
| EcoT22I | ATGCAT | 1 cut(s) | 817 |
| EcoT38I | GRGCYC | 1 cut(s) | 796 |
| ErhI | CCWWGG | 1 cut(s) | 700 |
| FaeI | CATG | 9 cut(s) | 26, 60, 86, 319, 819, 949, 1004, 1252, 1501 |
| FaqI | GGGAC | 2 cut(s) | 58, 434 |
| FatI | CATG | 9 cut(s) | 22, 56, 82, 315, 815, 945, 1000, 1248, 1497 |
| FauI | CCCGC | 2 cut(s) | 387, 1009 |
| FbaI | TGATCA | 1 cut(s) | 103 |
| Fnu4HI | GCNGC | 8 cut(s) | 171, 357, 495, 546, 549, 580, 1125, 1137 |
| FokI | GGATG | 1 cut(s) | 1202 |
| FriOI | GRGCYC | 1 cut(s) | 796 |
| Fsp4HI | GCNGC | 8 cut(s) | 171, 357, 495, 546, 549, 580, 1125, 1137 |
| FspBI | CTAG | 6 cut(s) | 632, 770, 900, 1029, 1275, 1585 |
| GluI | GCNGC | 8 cut(s) | 171, 357, 495, 546, 549, 580, 1125, 1137 |
| GsuI | CTGGAG | 1 cut(s) | 708 |
| HaeIII | GGCC | 4 cut(s) | 280, 410, 510, 1152 |
| HgaI | GACGC | 2 cut(s) | 176, 464 |
| Hin1II | CATG | 9 cut(s) | 26, 60, 86, 319, 819, 949, 1004, 1252, 1501 |
| HincII | GTYRAC | 1 cut(s) | 859 |
| HindII | GTYRAC | 1 cut(s) | 859 |
| HinfI | GANTC | 4 cut(s) | 146, 1219, 1261, 1328 |
| HphI | GGTGA | 8 cut(s) | 414, 436, 668, 689, 719, 742, 1186, 1367 |
| Hpy166II | GTNNAC | 3 cut(s) | 422, 629, 859 |
| Hpy188I | TCNGA | 2 cut(s) | 151, 1164 |
| Hpy188III | TCNNGA | 8 cut(s) | 383, 716, 754, 770, 1029, 1100, 1157, 1172 |
| Hpy8I | GTNNAC | 3 cut(s) | 422, 629, 859 |
| Hpy99I | CGWCG | 1 cut(s) | 170 |
| HpyAV | CCTTC | 4 cut(s) | 290, 670, 817, 1069 |
| HpyCH4III | ACNGT | 2 cut(s) | 448, 1535 |
| HpyCH4IV | ACGT | 1 cut(s) | 516 |
| HpyF10VI | GCNNNNNNNGC | 6 cut(s) | 503, 554, 588, 1121, 1133, 1398 |
| HpyF3I | CTNAG | 6 cut(s) | 253, 306, 405, 797, 1437, 1486 |
| HpySE526I | ACGT | 1 cut(s) | 516 |
| Hsp92II | CATG | 9 cut(s) | 26, 60, 86, 319, 819, 949, 1004, 1252, 1501 |
| Ksp22I | TGATCA | 1 cut(s) | 103 |
| Kzo9I | GATC | 5 cut(s) | 103, 183, 712, 1159, 1527 |
| LguI | GCTCTTC | 2 cut(s) | 1030, 1286 |
| LmnI | GCTCC | 5 cut(s) | 73, 151, 1078, 1121, 1426 |
| Lsp1109I | GCAGC | 6 cut(s) | 157, 343, 557, 560, 1111, 1123 |
| LweI | GCATC | 7 cut(s) | 205, 238, 381, 481, 510, 964, 1453 |
| MaeI | CTAG | 6 cut(s) | 632, 770, 900, 1029, 1275, 1585 |
| MaeII | ACGT | 1 cut(s) | 516 |
| MaeIII | GTNAC | 5 cut(s) | 325, 499, 1192, 1203, 1535 |
| MalI | GATC | 5 cut(s) | 105, 185, 714, 1161, 1529 |
| MboI | GATC | 5 cut(s) | 103, 183, 712, 1159, 1527 |
| MboII | GAAGA | 8 cut(s) | 202, 353, 722, 997, 1017, 1057, 1303, 1554 |
| MflI | RGATCY | 1 cut(s) | 712 |
| MhlI | GDGCHC | 2 cut(s) | 78, 796 |
| MlsI | TGGCCA | 1 cut(s) | 280 |
| MluNI | TGGCCA | 1 cut(s) | 280 |
| MlyI | GAGTC | 3 cut(s) | 140, 1228, 1337 |
| MmeI | TCCRAC | 1 cut(s) | 1194 |
| Mox20I | TGGCCA | 1 cut(s) | 280 |
| Mph1103I | ATGCAT | 1 cut(s) | 817 |
| MscI | TGGCCA | 1 cut(s) | 280 |
| MseI | TTAA | 9 cut(s) | 177, 189, 227, 270, 806, 968, 1055, 1095, 1455 |
| MslI | CAYNNNNRTG | 1 cut(s) | 81 |
| Msp20I | TGGCCA | 1 cut(s) | 280 |
| MspA1I | CMGCKG | 1 cut(s) | 686 |
| MwoI | GCNNNNNNNGC | 6 cut(s) | 503, 554, 588, 1121, 1133, 1398 |
| NdeII | GATC | 5 cut(s) | 103, 183, 712, 1159, 1527 |
| NlaIII | CATG | 9 cut(s) | 26, 60, 86, 319, 819, 949, 1004, 1252, 1501 |
| NlaIV | GGNNCC | 2 cut(s) | 951, 1422 |
| NmuCI | GTSAC | 4 cut(s) | 499, 1192, 1203, 1535 |
| NsiI | ATGCAT | 1 cut(s) | 817 |
| NspI | RCATGY | 2 cut(s) | 86, 819 |
| NspV | TTCGAA | 1 cut(s) | 669 |
| PceI | AGGCCT | 1 cut(s) | 410 |
| PciSI | GCTCTTC | 2 cut(s) | 1030, 1286 |
| PfeI | GAWTC | 1 cut(s) | 1261 |
| PkrI | GCNGC | 8 cut(s) | 172, 358, 496, 547, 550, 581, 1126, 1138 |
| PleI | GAGTC | 3 cut(s) | 140, 1227, 1336 |
| PpsI | GAGTC | 3 cut(s) | 140, 1227, 1336 |
| PpuMI | RGGWCCY | 1 cut(s) | 299 |
| PsiI | TTATAA | 1 cut(s) | 1304 |
| Psp124BI | GAGCTC | 1 cut(s) | 796 |
| Psp5II | RGGWCCY | 1 cut(s) | 299 |
| PspN4I | GGNNCC | 2 cut(s) | 951, 1422 |
| PspPI | GGNCC | 2 cut(s) | 299, 949 |
| PspPPI | RGGWCCY | 1 cut(s) | 299 |
| PstI | CTGCAG | 2 cut(s) | 547, 1141 |
| PstNI | CAGNNNCTG | 1 cut(s) | 503 |
| PsuI | RGATCY | 1 cut(s) | 712 |
| PvuII | CAGCTG | 1 cut(s) | 686 |
| RsaI | GTAC | 1 cut(s) | 628 |
| RsaNI | GTAC | 1 cut(s) | 627 |
| RseI | CAYNNNNRTG | 1 cut(s) | 81 |
| SacI | GAGCTC | 1 cut(s) | 796 |
| SapI | GCTCTTC | 2 cut(s) | 1030, 1286 |
| SaqAI | TTAA | 9 cut(s) | 177, 189, 227, 270, 806, 968, 1055, 1095, 1455 |
| SatI | GCNGC | 8 cut(s) | 171, 357, 495, 546, 549, 580, 1125, 1137 |
| Sau3AI | GATC | 5 cut(s) | 103, 183, 712, 1159, 1527 |
| Sau96I | GGNCC | 2 cut(s) | 299, 949 |
| SchI | GAGTC | 3 cut(s) | 140, 1228, 1337 |
| SduI | GDGCHC | 2 cut(s) | 78, 796 |
| SfaNI | GCATC | 7 cut(s) | 205, 238, 381, 481, 510, 964, 1453 |
| SfcI | CTRYAG | 3 cut(s) | 543, 681, 1137 |
| SfuI | TTCGAA | 1 cut(s) | 669 |
| SinI | GGWCC | 2 cut(s) | 299, 949 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 81 |
| SmlI | CTYRAG | 1 cut(s) | 1238 |
| SmoI | CTYRAG | 1 cut(s) | 1238 |
| SseBI | AGGCCT | 1 cut(s) | 410 |
| SsiI | CCGC | 7 cut(s) | 6, 394, 495, 579, 621, 1016, 1127 |
| SspI | AATATT | 1 cut(s) | 1459 |
| SspMI | CTAG | 6 cut(s) | 632, 770, 900, 1029, 1275, 1585 |
| SstI | GAGCTC | 1 cut(s) | 796 |
| StuI | AGGCCT | 1 cut(s) | 410 |
| StyI | CCWWGG | 1 cut(s) | 700 |
| TaaI | ACNGT | 2 cut(s) | 448, 1535 |
| TaiI | ACGT | 1 cut(s) | 519 |
| TaqI | TCGA | 3 cut(s) | 213, 669, 1581 |
| TaqII | GACCGA | 1 cut(s) | 1321 |
| TauI | GCSGC | 2 cut(s) | 497, 582 |
| TfiI | GAWTC | 1 cut(s) | 1261 |
| Tru1I | TTAA | 9 cut(s) | 177, 189, 227, 270, 806, 968, 1055, 1095, 1455 |
| Tru9I | TTAA | 9 cut(s) | 177, 189, 227, 270, 806, 968, 1055, 1095, 1455 |
| TscAI | CASTG | 4 cut(s) | 72, 508, 784, 1540 |
| TseFI | GTSAC | 4 cut(s) | 499, 1192, 1203, 1535 |
| TseI | GCWGC | 6 cut(s) | 170, 356, 545, 548, 1124, 1136 |
| Tsp45I | GTSAC | 4 cut(s) | 499, 1192, 1203, 1535 |
| TspDTI | ATGAA | 5 cut(s) | 45, 831, 998, 1265, 1304 |
| TspRI | CASTG | 4 cut(s) | 72, 508, 784, 1540 |
| VpaK11BI | GGWCC | 2 cut(s) | 299, 949 |
| XapI | RAATTY | 3 cut(s) | 749, 1032, 1546 |
| XbaI | TCTAGA | 2 cut(s) | 769, 1028 |
| XceI | RCATGY | 2 cut(s) | 86, 819 |
| XspI | CTAG | 6 cut(s) | 632, 770, 900, 1029, 1275, 1585 |
| Zsp2I | ATGCAT | 1 cut(s) | 817 |