Heat shock cognate 70 kDa
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 467 |
| AccB1I | GGYRCC | 1 cut(s) | 1160 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 1024 |
| AciI | CCGC | 4 cut(s) | 160, 260, 460, 483 |
| AclI | AACGTT | 1 cut(s) | 1097 |
| AclWI | GGATC | 1 cut(s) | 95 |
| AcoI | YGGCCR | 1 cut(s) | 215 |
| AcsI | RAATTY | 4 cut(s) | 209, 857, 981, 1135 |
| AfaI | GTAC | 5 cut(s) | 565, 948, 993, 1057, 1076 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 1024 |
| AhdI | GACNNNNNGTC | 1 cut(s) | 49 |
| AhlI | ACTAGT | 1 cut(s) | 839 |
| AjnI | CCWGG | 2 cut(s) | 238, 1017 |
| AjuI | GAANNNNNNNTTGG | 4 cut(s) | 689, 721, 1097, 1129 |
| AleI | CACNNNNGTG | 1 cut(s) | 1069 |
| AluBI | AGCT | 2 cut(s) | 341, 731 |
| AluI | AGCT | 2 cut(s) | 341, 731 |
| Alw21I | GWGCWC | 1 cut(s) | 733 |
| Alw26I | GTCTC | 4 cut(s) | 63, 621, 766, 785 |
| AlwI | GGATC | 1 cut(s) | 95 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 794 |
| Ama87I | CYCGRG | 1 cut(s) | 849 |
| AoxI | GGCC | 8 cut(s) | 110, 215, 248, 345, 445, 499, 513, 615 |
| ApeKI | GCWGC | 2 cut(s) | 293, 485 |
| ApoI | RAATTY | 4 cut(s) | 209, 857, 981, 1135 |
| AseI | ATTAAT | 1 cut(s) | 1044 |
| AspA2I | CCTAGG | 1 cut(s) | 148 |
| AspS9I | GGNCC | 4 cut(s) | 110, 236, 249, 709 |
| AsuHPI | GGTGA | 6 cut(s) | 254, 287, 626, 656, 679, 1056 |
| AsuII | TTCGAA | 1 cut(s) | 1139 |
| AvaI | CYCGRG | 1 cut(s) | 849 |
| AvaII | GGWCC | 2 cut(s) | 236, 709 |
| AvrII | CCTAGG | 1 cut(s) | 148 |
| BalI | TGGCCA | 1 cut(s) | 217 |
| BanI | GGYRCC | 1 cut(s) | 1160 |
| BanII | GRGCYC | 1 cut(s) | 733 |
| BbsI | GAAGAC | 1 cut(s) | 654 |
| Bbv12I | GWGCWC | 1 cut(s) | 733 |
| BbvCI | CCTCAGC | 1 cut(s) | 342 |
| BbvI | GCAGC | 2 cut(s) | 280, 497 |
| BccI | CCATC | 1 cut(s) | 1018 |
| BciT130I | CCWGG | 2 cut(s) | 240, 1019 |
| BcoDI | GTCTC | 4 cut(s) | 63, 621, 766, 785 |
| BcuI | ACTAGT | 1 cut(s) | 839 |
| BfaI | CTAG | 4 cut(s) | 66, 149, 840, 978 |
| BfmI | CTRYAG | 1 cut(s) | 294 |
| BisI | GCNGC | 3 cut(s) | 294, 483, 486 |
| BlnI | CCTAGG | 1 cut(s) | 148 |
| BlsI | GCNGC | 3 cut(s) | 295, 484, 487 |
| Bme1390I | CCNGG | 2 cut(s) | 240, 1019 |
| Bme18I | GGWCC | 2 cut(s) | 236, 709 |
| BmeRI | GACNNNNNGTC | 1 cut(s) | 49 |
| BmeT110I | CYCGRG | 1 cut(s) | 849 |
| BmgT120I | GGNCC | 4 cut(s) | 110, 236, 249, 709 |
| BmiI | GGNNCC | 3 cut(s) | 111, 900, 1162 |
| BmrFI | CCNGG | 2 cut(s) | 240, 1019 |
| BmsI | GCATC | 6 cut(s) | 69, 318, 418, 447, 826, 913 |
| BpiI | GAAGAC | 1 cut(s) | 654 |
| BpmI | CTGGAG | 3 cut(s) | 645, 709, 938 |
| Bpu10I | CCTNAGC | 1 cut(s) | 342 |
| Bpu14I | TTCGAA | 1 cut(s) | 1139 |
| BsaJI | CCNNGG | 6 cut(s) | 113, 148, 312, 352, 637, 1018 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 166, 196 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 1024 |
| Bse1I | ACTGG | 4 cut(s) | 135, 516, 598, 1164 |
| Bse3DI | GCAATG | 1 cut(s) | 438 |
| BseBI | CCWGG | 2 cut(s) | 240, 1019 |
| BseDI | CCNNGG | 6 cut(s) | 113, 148, 312, 352, 637, 1018 |
| BseGI | GGATG | 4 cut(s) | 60, 308, 928, 1029 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 1024 |
| BseMI | GCAATG | 1 cut(s) | 438 |
| BseMII | CTCAG | 1 cut(s) | 333 |
| BseNI | ACTGG | 4 cut(s) | 135, 516, 598, 1164 |
| BseRI | GAGGAG | 2 cut(s) | 784, 1073 |
| BseXI | GCAGC | 2 cut(s) | 280, 497 |
| BshFI | GGCC | 8 cut(s) | 112, 217, 250, 347, 447, 501, 515, 617 |
| BshNI | GGYRCC | 1 cut(s) | 1160 |
| BsiHKAI | GWGCWC | 1 cut(s) | 733 |
| BsiHKCI | CYCGRG | 1 cut(s) | 849 |
| BslFI | GGGAC | 1 cut(s) | 371 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 1024 |
| BsmAI | GTCTC | 4 cut(s) | 63, 621, 766, 785 |
| BsmFI | GGGAC | 1 cut(s) | 371 |
| BsnI | GGCC | 8 cut(s) | 112, 217, 250, 347, 447, 501, 515, 617 |
| BsoBI | CYCGRG | 1 cut(s) | 849 |
| Bsp119I | TTCGAA | 1 cut(s) | 1139 |
| Bsp1286I | GDGCHC | 1 cut(s) | 733 |
| Bsp1407I | TGTACA | 1 cut(s) | 946 |
| Bsp143I | GATC | 1 cut(s) | 100 |
| Bsp19I | CCATGG | 2 cut(s) | 113, 312 |
| BspACI | CCGC | 4 cut(s) | 160, 260, 460, 483 |
| BspANI | GGCC | 8 cut(s) | 112, 217, 250, 347, 447, 501, 515, 617 |
| BspCNI | CTCAG | 1 cut(s) | 334 |
| BspLI | GGNNCC | 3 cut(s) | 111, 900, 1162 |
| BspMAI | CTGCAG | 1 cut(s) | 298 |
| BspPI | GGATC | 1 cut(s) | 95 |
| BspQI | GCTCTTC | 1 cut(s) | 979 |
| BspT104I | TTCGAA | 1 cut(s) | 1139 |
| BspT107I | GGYRCC | 1 cut(s) | 1160 |
| BsrDI | GCAATG | 1 cut(s) | 438 |
| BsrGI | TGTACA | 1 cut(s) | 946 |
| BsrI | ACTGG | 4 cut(s) | 135, 516, 598, 1164 |
| BssECI | CCNNGG | 6 cut(s) | 113, 148, 312, 352, 637, 1018 |
| BssMI | GATC | 1 cut(s) | 100 |
| BssT1I | CCWWGG | 5 cut(s) | 113, 148, 312, 352, 637 |
| Bst2UI | CCWGG | 2 cut(s) | 240, 1019 |
| Bst4CI | ACNGT | 4 cut(s) | 364, 385, 677, 812 |
| Bst6I | CTCTTC | 1 cut(s) | 979 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 491 |
| BstAUI | TGTACA | 1 cut(s) | 946 |
| BstBI | TTCGAA | 1 cut(s) | 1139 |
| BstC8I | GCNNGC | 2 cut(s) | 445, 754 |
| BstDEI | CTNAG | 3 cut(s) | 342, 713, 734 |
| BstDSI | CCRYGG | 2 cut(s) | 113, 312 |
| BstEII | GGTNACC | 1 cut(s) | 262 |
| BstF5I | GGATG | 4 cut(s) | 60, 308, 928, 1029 |
| BstKTI | GATC | 1 cut(s) | 103 |
| BstMAI | GTCTC | 4 cut(s) | 63, 621, 766, 785 |
| BstMBI | GATC | 1 cut(s) | 100 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 444, 491 |
| BstNI | CCWGG | 2 cut(s) | 240, 1019 |
| BstPI | GGTNACC | 1 cut(s) | 262 |
| BstSCI | CCNGG | 2 cut(s) | 238, 1017 |
| BstSFI | CTRYAG | 1 cut(s) | 294 |
| BstV1I | GCAGC | 2 cut(s) | 280, 497 |
| BstV2I | GAAGAC | 1 cut(s) | 654 |
| BstX2I | RGATCY | 1 cut(s) | 100 |
| BstYI | RGATCY | 1 cut(s) | 100 |
| BsuRI | GGCC | 8 cut(s) | 112, 217, 250, 347, 447, 501, 515, 617 |
| BtgI | CCRYGG | 2 cut(s) | 113, 312 |
| BtsCI | GGATG | 4 cut(s) | 60, 308, 928, 1029 |
| BtsI | GCAGTG | 1 cut(s) | 949 |
| BtsIMutI | CAGTG | 4 cut(s) | 187, 523, 949, 1171 |
| Cac8I | GCNNGC | 2 cut(s) | 445, 754 |
| CaiI | CAGNNNCTG | 1 cut(s) | 794 |
| Cfr13I | GGNCC | 4 cut(s) | 110, 236, 249, 709 |
| CseI | GACGC | 1 cut(s) | 401 |
| Csp6I | GTAC | 5 cut(s) | 564, 947, 992, 1056, 1075 |
| CviAII | CATG | 4 cut(s) | 114, 313, 874, 950 |
| CviQI | GTAC | 5 cut(s) | 564, 947, 992, 1056, 1075 |
| DdeI | CTNAG | 3 cut(s) | 342, 713, 734 |
| DpnI | GATC | 1 cut(s) | 102 |
| DpnII | GATC | 1 cut(s) | 100 |
| DriI | GACNNNNNGTC | 1 cut(s) | 49 |
| EaeI | YGGCCR | 1 cut(s) | 215 |
| Eam1104I | CTCTTC | 1 cut(s) | 979 |
| Eam1105I | GACNNNNNGTC | 1 cut(s) | 49 |
| EarI | CTCTTC | 1 cut(s) | 979 |
| Ecl136II | GAGCTC | 1 cut(s) | 731 |
| Eco130I | CCWWGG | 5 cut(s) | 113, 148, 312, 352, 637 |
| Eco147I | AGGCCT | 1 cut(s) | 347 |
| Eco24I | GRGCYC | 1 cut(s) | 733 |
| Eco47I | GGWCC | 2 cut(s) | 236, 709 |
| Eco53kI | GAGCTC | 1 cut(s) | 731 |
| Eco88I | CYCGRG | 1 cut(s) | 849 |
| Eco91I | GGTNACC | 1 cut(s) | 262 |
| EcoICRI | GAGCTC | 1 cut(s) | 731 |
| EcoO109I | RGGNCCY | 2 cut(s) | 236, 249 |
| EcoO65I | GGTNACC | 1 cut(s) | 262 |
| EcoRI | GAATTC | 1 cut(s) | 981 |
| EcoRII | CCWGG | 2 cut(s) | 238, 1017 |
| EcoT14I | CCWWGG | 5 cut(s) | 113, 148, 312, 352, 637 |
| EcoT38I | GRGCYC | 1 cut(s) | 733 |
| ErhI | CCWWGG | 5 cut(s) | 113, 148, 312, 352, 637 |
| FaeI | CATG | 4 cut(s) | 117, 316, 877, 953 |
| FaqI | GGGAC | 1 cut(s) | 371 |
| FatI | CATG | 4 cut(s) | 113, 312, 873, 949 |
| Fnu4HI | GCNGC | 3 cut(s) | 294, 483, 486 |
| FokI | GGATG | 4 cut(s) | 47, 295, 935, 1036 |
| FriOI | GRGCYC | 1 cut(s) | 733 |
| Fsp4HI | GCNGC | 3 cut(s) | 294, 483, 486 |
| FspBI | CTAG | 4 cut(s) | 66, 149, 840, 978 |
| GluI | GCNGC | 3 cut(s) | 294, 483, 486 |
| GsuI | CTGGAG | 3 cut(s) | 645, 709, 938 |
| HaeIII | GGCC | 8 cut(s) | 112, 217, 250, 347, 447, 501, 515, 617 |
| HgaI | GACGC | 1 cut(s) | 401 |
| Hin1II | CATG | 4 cut(s) | 117, 316, 877, 953 |
| HinfI | GANTC | 4 cut(s) | 83, 177, 185, 404 |
| HphI | GGTGA | 6 cut(s) | 254, 287, 626, 656, 679, 1056 |
| Hpy166II | GTNNAC | 2 cut(s) | 947, 1058 |
| Hpy188I | TCNGA | 2 cut(s) | 198, 323 |
| Hpy188III | TCNNGA | 4 cut(s) | 624, 726, 917, 978 |
| Hpy8I | GTNNAC | 2 cut(s) | 947, 1058 |
| HpyAV | CCTTC | 4 cut(s) | 227, 505, 607, 754 |
| HpyCH4III | ACNGT | 4 cut(s) | 364, 385, 677, 812 |
| HpyCH4IV | ACGT | 1 cut(s) | 1097 |
| HpyCH4V | TGCA | 7 cut(s) | 155, 296, 374, 817, 926, 1006, 1035 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 444, 491 |
| HpyF3I | CTNAG | 3 cut(s) | 342, 713, 734 |
| HpySE526I | ACGT | 1 cut(s) | 1097 |
| Hsp92II | CATG | 4 cut(s) | 117, 316, 877, 953 |
| Kzo9I | GATC | 1 cut(s) | 100 |
| LguI | GCTCTTC | 1 cut(s) | 979 |
| LmnI | GCTCC | 1 cut(s) | 898 |
| Lsp1109I | GCAGC | 2 cut(s) | 280, 497 |
| LweI | GCATC | 6 cut(s) | 69, 318, 418, 447, 826, 913 |
| MaeI | CTAG | 4 cut(s) | 66, 149, 840, 978 |
| MaeII | ACGT | 1 cut(s) | 1097 |
| MaeIII | GTNAC | 2 cut(s) | 242, 262 |
| MalI | GATC | 1 cut(s) | 102 |
| MboI | GATC | 1 cut(s) | 100 |
| MboII | GAAGA | 6 cut(s) | 290, 311, 659, 874, 966, 1108 |
| MflI | RGATCY | 1 cut(s) | 100 |
| MhlI | GDGCHC | 1 cut(s) | 733 |
| MlsI | TGGCCA | 1 cut(s) | 217 |
| MluNI | TGGCCA | 1 cut(s) | 217 |
| MlyI | GAGTC | 1 cut(s) | 92 |
| Mox20I | TGGCCA | 1 cut(s) | 217 |
| MscI | TGGCCA | 1 cut(s) | 217 |
| MseI | TTAA | 5 cut(s) | 164, 690, 743, 1044, 1173 |
| MslI | CAYNNNNRTG | 1 cut(s) | 1069 |
| Msp20I | TGGCCA | 1 cut(s) | 217 |
| MspR9I | CCNGG | 2 cut(s) | 240, 1019 |
| MvaI | CCWGG | 2 cut(s) | 240, 1019 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 444, 491 |
| NcoI | CCATGG | 2 cut(s) | 113, 312 |
| NdeII | GATC | 1 cut(s) | 100 |
| NlaIII | CATG | 4 cut(s) | 117, 316, 877, 953 |
| NlaIV | GGNNCC | 3 cut(s) | 111, 900, 1162 |
| NmuCI | GTSAC | 1 cut(s) | 242 |
| NspV | TTCGAA | 1 cut(s) | 1139 |
| OliI | CACNNNNGTG | 1 cut(s) | 1069 |
| PaeR7I | CTCGAG | 1 cut(s) | 849 |
| PceI | AGGCCT | 1 cut(s) | 347 |
| PciSI | GCTCTTC | 1 cut(s) | 979 |
| PfeI | GAWTC | 3 cut(s) | 177, 185, 404 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 1024 |
| PkrI | GCNGC | 3 cut(s) | 295, 484, 487 |
| PleI | GAGTC | 1 cut(s) | 91 |
| PpsI | GAGTC | 1 cut(s) | 91 |
| PpuMI | RGGWCCY | 1 cut(s) | 236 |
| PshBI | ATTAAT | 1 cut(s) | 1044 |
| PsiI | TTATAA | 1 cut(s) | 467 |
| Psp124BI | GAGCTC | 1 cut(s) | 733 |
| Psp1406I | AACGTT | 1 cut(s) | 1097 |
| Psp5II | RGGWCCY | 1 cut(s) | 236 |
| Psp6I | CCWGG | 2 cut(s) | 238, 1017 |
| PspEI | GGTNACC | 1 cut(s) | 262 |
| PspGI | CCWGG | 2 cut(s) | 238, 1017 |
| PspN4I | GGNNCC | 3 cut(s) | 111, 900, 1162 |
| PspPI | GGNCC | 4 cut(s) | 110, 236, 249, 709 |
| PspPPI | RGGWCCY | 1 cut(s) | 236 |
| PspXI | VCTCGAGB | 1 cut(s) | 849 |
| PstI | CTGCAG | 1 cut(s) | 298 |
| PstNI | CAGNNNCTG | 1 cut(s) | 794 |
| PsuI | RGATCY | 1 cut(s) | 100 |
| RsaI | GTAC | 5 cut(s) | 565, 948, 993, 1057, 1076 |
| RsaNI | GTAC | 5 cut(s) | 564, 947, 992, 1056, 1075 |
| RseI | CAYNNNNRTG | 1 cut(s) | 1069 |
| SacI | GAGCTC | 1 cut(s) | 733 |
| SapI | GCTCTTC | 1 cut(s) | 979 |
| SaqAI | TTAA | 5 cut(s) | 164, 690, 743, 1044, 1173 |
| SatI | GCNGC | 3 cut(s) | 294, 483, 486 |
| Sau3AI | GATC | 1 cut(s) | 100 |
| Sau96I | GGNCC | 4 cut(s) | 110, 236, 249, 709 |
| SchI | GAGTC | 1 cut(s) | 92 |
| ScrFI | CCNGG | 2 cut(s) | 240, 1019 |
| SduI | GDGCHC | 1 cut(s) | 733 |
| SfaNI | GCATC | 6 cut(s) | 69, 318, 418, 447, 826, 913 |
| SfcI | CTRYAG | 1 cut(s) | 294 |
| Sfr274I | CTCGAG | 1 cut(s) | 849 |
| SfuI | TTCGAA | 1 cut(s) | 1139 |
| SinI | GGWCC | 2 cut(s) | 236, 709 |
| SlaI | CTCGAG | 1 cut(s) | 849 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 1069 |
| SmlI | CTYRAG | 1 cut(s) | 849 |
| SmoI | CTYRAG | 1 cut(s) | 849 |
| SpeI | ACTAGT | 1 cut(s) | 839 |
| SseBI | AGGCCT | 1 cut(s) | 347 |
| SsiI | CCGC | 4 cut(s) | 160, 260, 460, 483 |
| SspMI | CTAG | 4 cut(s) | 66, 149, 840, 978 |
| SstI | GAGCTC | 1 cut(s) | 733 |
| StuI | AGGCCT | 1 cut(s) | 347 |
| StyD4I | CCNGG | 2 cut(s) | 238, 1017 |
| StyI | CCWWGG | 5 cut(s) | 113, 148, 312, 352, 637 |
| TaaI | ACNGT | 4 cut(s) | 364, 385, 677, 812 |
| TaiI | ACGT | 1 cut(s) | 1100 |
| TaqI | TCGA | 3 cut(s) | 654, 850, 1139 |
| TatI | WGTACW | 2 cut(s) | 946, 991 |
| TauI | GCSGC | 1 cut(s) | 485 |
| TfiI | GAWTC | 3 cut(s) | 177, 185, 404 |
| Tru1I | TTAA | 5 cut(s) | 164, 690, 743, 1044, 1173 |
| Tru9I | TTAA | 5 cut(s) | 164, 690, 743, 1044, 1173 |
| TscAI | CASTG | 4 cut(s) | 187, 523, 949, 1171 |
| TseFI | GTSAC | 1 cut(s) | 242 |
| TseI | GCWGC | 2 cut(s) | 293, 485 |
| Tsp45I | GTSAC | 1 cut(s) | 242 |
| TspDTI | ATGAA | 2 cut(s) | 137, 486 |
| TspRI | CASTG | 4 cut(s) | 187, 523, 949, 1171 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 1024 |
| VpaK11BI | GGWCC | 2 cut(s) | 236, 709 |
| VspI | ATTAAT | 1 cut(s) | 1044 |
| XapI | RAATTY | 4 cut(s) | 209, 857, 981, 1135 |
| XbaI | TCTAGA | 1 cut(s) | 977 |
| XhoI | CTCGAG | 1 cut(s) | 849 |
| XmaJI | CCTAGG | 1 cut(s) | 148 |
| XspI | CTAG | 4 cut(s) | 66, 149, 840, 978 |