Heat shock cognate 70 kDa
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AasI | GACNNNNNNGTC | 1 cut(s) | 958 |
| Acc36I | ACCTGC | 1 cut(s) | 43 |
| AccB1I | GGYRCC | 1 cut(s) | 875 |
| AccII | CGCG | 1 cut(s) | 281 |
| AciI | CCGC | 7 cut(s) | 64, 69, 279, 281, 360, 428, 879 |
| AclWI | GGATC | 1 cut(s) | 1283 |
| AcoI | YGGCCR | 2 cut(s) | 11, 1261 |
| AcsI | RAATTY | 5 cut(s) | 526, 602, 662, 1148, 1193 |
| AcuI | CTGAAG | 2 cut(s) | 159, 1192 |
| AdeI | CACNNNGTG | 1 cut(s) | 968 |
| AfaI | GTAC | 2 cut(s) | 367, 631 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 1263 |
| AflIII | ACRYGT | 1 cut(s) | 1234 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 132, 164 |
| AluBI | AGCT | 6 cut(s) | 215, 805, 833, 925, 1175, 1301 |
| AluI | AGCT | 6 cut(s) | 215, 805, 833, 925, 1175, 1301 |
| Alw26I | GTCTC | 2 cut(s) | 426, 1065 |
| AlwI | GGATC | 1 cut(s) | 1283 |
| AlwNI | CAGNNNCTG | 2 cut(s) | 185, 1178 |
| Ama87I | CYCGRG | 2 cut(s) | 654, 917 |
| AoxI | GGCC | 5 cut(s) | 11, 241, 420, 657, 1261 |
| ApeKI | GCWGC | 5 cut(s) | 66, 253, 799, 802, 890 |
| ApoI | RAATTY | 5 cut(s) | 526, 602, 662, 1148, 1193 |
| AseI | ATTAAT | 2 cut(s) | 393, 849 |
| AspS9I | GGNCC | 2 cut(s) | 32, 657 |
| AsuC2I | CCSGG | 2 cut(s) | 918, 919 |
| AsuHPI | GGTGA | 4 cut(s) | 379, 461, 1157, 1196 |
| AsuII | TTCGAA | 1 cut(s) | 666 |
| AvaI | CYCGRG | 2 cut(s) | 654, 917 |
| AvaII | GGWCC | 1 cut(s) | 32 |
| AxyI | CCTNAGG | 1 cut(s) | 1179 |
| BalI | TGGCCA | 1 cut(s) | 13 |
| BanI | GGYRCC | 1 cut(s) | 875 |
| BbsI | GAAGAC | 3 cut(s) | 459, 583, 980 |
| BbvCI | CCTCAGC | 1 cut(s) | 1245 |
| BbvI | GCAGC | 5 cut(s) | 78, 240, 811, 814, 877 |
| BccI | CCATC | 4 cut(s) | 337, 395, 1046, 1154 |
| BceAI | ACGGC | 2 cut(s) | 478, 878 |
| BcgI | CGANNNNNNTGC | 4 cut(s) | 17, 51, 257, 291 |
| BcnI | CCSGG | 2 cut(s) | 918, 919 |
| BcoDI | GTCTC | 2 cut(s) | 426, 1065 |
| BfaI | CTAG | 2 cut(s) | 312, 783 |
| BfmI | CTRYAG | 2 cut(s) | 36, 864 |
| BfuAI | ACCTGC | 1 cut(s) | 43 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 620 |
| BisI | GCNGC | 8 cut(s) | 67, 254, 279, 282, 800, 803, 879, 891 |
| BlsI | GCNGC | 8 cut(s) | 68, 255, 280, 283, 801, 804, 880, 892 |
| Bme1390I | CCNGG | 2 cut(s) | 918, 919 |
| Bme18I | GGWCC | 1 cut(s) | 32 |
| BmeT110I | CYCGRG | 2 cut(s) | 654, 917 |
| BmgT120I | GGNCC | 2 cut(s) | 32, 657 |
| BmiI | GGNNCC | 2 cut(s) | 705, 877 |
| BmrFI | CCNGG | 2 cut(s) | 918, 919 |
| BmsI | GCATC | 4 cut(s) | 114, 718, 1107, 1207 |
| BpiI | GAAGAC | 3 cut(s) | 459, 583, 980 |
| BpmI | CTGGAG | 1 cut(s) | 1155 |
| Bpu10I | CCTNAGC | 1 cut(s) | 1245 |
| Bpu14I | TTCGAA | 1 cut(s) | 666 |
| BpuMI | CCSGG | 2 cut(s) | 918, 919 |
| BsaBI | GATNNNNATC | 1 cut(s) | 1280 |
| BsaJI | CCNNGG | 4 cut(s) | 148, 279, 792, 917 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 1263 |
| Bse1I | ACTGG | 2 cut(s) | 707, 1264 |
| Bse21I | CCTNAGG | 1 cut(s) | 1179 |
| Bse3DI | GCAATG | 1 cut(s) | 380 |
| Bse8I | GATNNNNATC | 1 cut(s) | 1280 |
| BseDI | CCNNGG | 4 cut(s) | 148, 279, 792, 917 |
| BseGI | GGATG | 6 cut(s) | 60, 104, 520, 745, 760, 952 |
| BseJI | GATNNNNATC | 1 cut(s) | 1280 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 1263 |
| BseMI | GCAATG | 1 cut(s) | 380 |
| BseMII | CTCAG | 3 cut(s) | 130, 1170, 1236 |
| BseNI | ACTGG | 2 cut(s) | 707, 1264 |
| BseXI | GCAGC | 5 cut(s) | 78, 240, 811, 814, 877 |
| BsgI | GTGCAG | 1 cut(s) | 818 |
| Bsh1236I | CGCG | 1 cut(s) | 281 |
| BshFI | GGCC | 5 cut(s) | 13, 243, 422, 659, 1263 |
| BshNI | GGYRCC | 1 cut(s) | 875 |
| BsiHKCI | CYCGRG | 2 cut(s) | 654, 917 |
| BsiSI | CCGG | 2 cut(s) | 918, 1264 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 1263 |
| BsmAI | GTCTC | 2 cut(s) | 426, 1065 |
| BsmI | GAATGC | 3 cut(s) | 172, 563, 1164 |
| BsnI | GGCC | 5 cut(s) | 13, 243, 422, 659, 1263 |
| BsoBI | CYCGRG | 2 cut(s) | 654, 917 |
| Bsp119I | TTCGAA | 1 cut(s) | 666 |
| Bsp143I | GATC | 1 cut(s) | 1275 |
| BspACI | CCGC | 7 cut(s) | 64, 69, 279, 281, 360, 428, 879 |
| BspANI | GGCC | 5 cut(s) | 13, 243, 422, 659, 1263 |
| BspCNI | CTCAG | 3 cut(s) | 129, 1171, 1237 |
| BspFNI | CGCG | 1 cut(s) | 281 |
| BspLI | GGNNCC | 2 cut(s) | 705, 877 |
| BspMAI | CTGCAG | 1 cut(s) | 40 |
| BspMI | ACCTGC | 1 cut(s) | 43 |
| BspPI | GGATC | 1 cut(s) | 1283 |
| BspT104I | TTCGAA | 1 cut(s) | 666 |
| BspT107I | GGYRCC | 1 cut(s) | 875 |
| BsrDI | GCAATG | 1 cut(s) | 380 |
| BsrI | ACTGG | 2 cut(s) | 707, 1264 |
| BssECI | CCNNGG | 4 cut(s) | 148, 279, 792, 917 |
| BssMI | GATC | 1 cut(s) | 1275 |
| BssT1I | CCWWGG | 2 cut(s) | 148, 792 |
| Bst4CI | ACNGT | 7 cut(s) | 84, 160, 181, 340, 482, 979, 1289 |
| Bst6I | CTCTTC | 1 cut(s) | 494 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 875 |
| BstBI | TTCGAA | 1 cut(s) | 666 |
| BstC8I | GCNNGC | 2 cut(s) | 186, 241 |
| BstDEI | CTNAG | 3 cut(s) | 116, 1179, 1245 |
| BstDSI | CCRYGG | 1 cut(s) | 279 |
| BstF5I | GGATG | 6 cut(s) | 60, 104, 520, 745, 760, 952 |
| BstFNI | CGCG | 1 cut(s) | 281 |
| BstKTI | GATC | 1 cut(s) | 1278 |
| BstMAI | GTCTC | 2 cut(s) | 426, 1065 |
| BstMBI | GATC | 1 cut(s) | 1275 |
| BstMWI | GCNNNNNNNGC | 5 cut(s) | 287, 620, 875, 887, 922 |
| BstNSI | RCATGY | 1 cut(s) | 1238 |
| BstSCI | CCNGG | 2 cut(s) | 916, 917 |
| BstSFI | CTRYAG | 2 cut(s) | 36, 864 |
| BstUI | CGCG | 1 cut(s) | 281 |
| BstV1I | GCAGC | 5 cut(s) | 78, 240, 811, 814, 877 |
| BstV2I | GAAGAC | 3 cut(s) | 459, 583, 980 |
| Bsu36I | CCTNAGG | 1 cut(s) | 1179 |
| BsuRI | GGCC | 5 cut(s) | 13, 243, 422, 659, 1263 |
| BtgI | CCRYGG | 1 cut(s) | 279 |
| BtsCI | GGATG | 6 cut(s) | 60, 104, 520, 745, 760, 952 |
| BtsIMutI | CAGTG | 3 cut(s) | 186, 345, 1257 |
| BveI | ACCTGC | 1 cut(s) | 43 |
| Cac8I | GCNNGC | 2 cut(s) | 186, 241 |
| CaiI | CAGNNNCTG | 2 cut(s) | 185, 1178 |
| Cfr13I | GGNCC | 2 cut(s) | 32, 657 |
| Cfr42I | CCGCGG | 1 cut(s) | 282 |
| Cfr9I | CCCGGG | 1 cut(s) | 917 |
| Csp6I | GTAC | 2 cut(s) | 366, 630 |
| CviAII | CATG | 4 cut(s) | 469, 506, 679, 1235 |
| CviQI | GTAC | 2 cut(s) | 366, 630 |
| DdeI | CTNAG | 3 cut(s) | 116, 1179, 1245 |
| DpnI | GATC | 1 cut(s) | 1277 |
| DpnII | GATC | 1 cut(s) | 1275 |
| DraIII | CACNNNGTG | 1 cut(s) | 968 |
| DrdI | GACNNNNNNGTC | 1 cut(s) | 958 |
| DseDI | GACNNNNNNGTC | 1 cut(s) | 958 |
| EaeI | YGGCCR | 2 cut(s) | 11, 1261 |
| Eam1104I | CTCTTC | 1 cut(s) | 494 |
| EarI | CTCTTC | 1 cut(s) | 494 |
| Eco130I | CCWWGG | 2 cut(s) | 148, 792 |
| Eco47I | GGWCC | 1 cut(s) | 32 |
| Eco57I | CTGAAG | 2 cut(s) | 159, 1192 |
| Eco81I | CCTNAGG | 1 cut(s) | 1179 |
| Eco88I | CYCGRG | 2 cut(s) | 654, 917 |
| EcoO109I | RGGNCCY | 1 cut(s) | 32 |
| EcoT14I | CCWWGG | 2 cut(s) | 148, 792 |
| ErhI | CCWWGG | 2 cut(s) | 148, 792 |
| FaeI | CATG | 4 cut(s) | 472, 509, 682, 1238 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 132, 164 |
| FatI | CATG | 4 cut(s) | 468, 505, 678, 1234 |
| FauI | CCCGC | 1 cut(s) | 421 |
| FauNDI | CATATG | 1 cut(s) | 871 |
| Fnu4HI | GCNGC | 8 cut(s) | 67, 254, 279, 282, 800, 803, 879, 891 |
| FokI | GGATG | 6 cut(s) | 47, 91, 527, 752, 767, 959 |
| Fsp4HI | GCNGC | 8 cut(s) | 67, 254, 279, 282, 800, 803, 879, 891 |
| FspBI | CTAG | 2 cut(s) | 312, 783 |
| GluI | GCNGC | 8 cut(s) | 67, 254, 279, 282, 800, 803, 879, 891 |
| GsuI | CTGGAG | 1 cut(s) | 1155 |
| HaeIII | GGCC | 5 cut(s) | 13, 243, 422, 659, 1263 |
| HapII | CCGG | 2 cut(s) | 918, 1264 |
| Hin1II | CATG | 4 cut(s) | 472, 509, 682, 1238 |
| HincII | GTYRAC | 1 cut(s) | 475 |
| HindII | GTYRAC | 1 cut(s) | 475 |
| HindIII | AAGCTT | 2 cut(s) | 923, 1299 |
| HinfI | GANTC | 3 cut(s) | 200, 787, 1227 |
| HpaI | GTTAAC | 1 cut(s) | 475 |
| HpaII | CCGG | 2 cut(s) | 918, 1264 |
| HphI | GGTGA | 4 cut(s) | 379, 461, 1157, 1196 |
| Hpy166II | GTNNAC | 2 cut(s) | 87, 475 |
| Hpy188I | TCNGA | 2 cut(s) | 119, 946 |
| Hpy188III | TCNNGA | 8 cut(s) | 74, 92, 488, 496, 854, 898, 929, 1178 |
| Hpy8I | GTNNAC | 2 cut(s) | 87, 475 |
| Hpy99I | CGWCG | 1 cut(s) | 464 |
| HpyAV | CCTTC | 5 cut(s) | 23, 301, 412, 559, 1100 |
| HpyCH4III | ACNGT | 7 cut(s) | 84, 160, 181, 340, 482, 979, 1289 |
| HpyCH4IV | ACGT | 2 cut(s) | 208, 759 |
| HpyCH4V | TGCA | 5 cut(s) | 38, 799, 840, 1085, 1220 |
| HpyF10VI | GCNNNNNNNGC | 5 cut(s) | 287, 620, 875, 887, 922 |
| HpyF3I | CTNAG | 3 cut(s) | 116, 1179, 1245 |
| HpySE526I | ACGT | 2 cut(s) | 208, 759 |
| Hsp92II | CATG | 4 cut(s) | 472, 509, 682, 1238 |
| KspAI | GTTAAC | 1 cut(s) | 475 |
| KspI | CCGCGG | 1 cut(s) | 282 |
| Kzo9I | GATC | 1 cut(s) | 1275 |
| LmnI | GCTCC | 2 cut(s) | 703, 1180 |
| Lsp1109I | GCAGC | 5 cut(s) | 78, 240, 811, 814, 877 |
| LweI | GCATC | 4 cut(s) | 114, 718, 1107, 1207 |
| MaeI | CTAG | 2 cut(s) | 312, 783 |
| MaeII | ACGT | 2 cut(s) | 208, 759 |
| MaeIII | GTNAC | 5 cut(s) | 232, 949, 960, 1237, 1289 |
| MalI | GATC | 1 cut(s) | 1277 |
| MboI | GATC | 1 cut(s) | 1275 |
| MboII | GAAGA | 9 cut(s) | 107, 464, 511, 579, 588, 679, 737, 771, 985 |
| MfeI | CAATTG | 1 cut(s) | 285 |
| MlsI | TGGCCA | 1 cut(s) | 13 |
| MluCI | AATT | 8 cut(s) | 285, 526, 602, 662, 1032, 1148, 1155, 1193 |
| MluNI | TGGCCA | 1 cut(s) | 13 |
| MlyI | GAGTC | 1 cut(s) | 194 |
| Mox20I | TGGCCA | 1 cut(s) | 13 |
| MscI | TGGCCA | 1 cut(s) | 13 |
| MseI | TTAA | 3 cut(s) | 393, 474, 849 |
| MslI | CAYNNNNRTG | 1 cut(s) | 179 |
| Msp20I | TGGCCA | 1 cut(s) | 13 |
| MspA1I | CMGCKG | 4 cut(s) | 69, 281, 428, 881 |
| MspI | CCGG | 2 cut(s) | 918, 1264 |
| MspR9I | CCNGG | 2 cut(s) | 918, 919 |
| MunI | CAATTG | 1 cut(s) | 285 |
| Mva1269I | GAATGC | 3 cut(s) | 172, 563, 1164 |
| MvnI | CGCG | 1 cut(s) | 281 |
| MwoI | GCNNNNNNNGC | 5 cut(s) | 287, 620, 875, 887, 922 |
| NciI | CCSGG | 2 cut(s) | 918, 919 |
| NdeI | CATATG | 1 cut(s) | 871 |
| NdeII | GATC | 1 cut(s) | 1275 |
| NlaIII | CATG | 4 cut(s) | 472, 509, 682, 1238 |
| NlaIV | GGNNCC | 2 cut(s) | 705, 877 |
| NmuCI | GTSAC | 3 cut(s) | 949, 960, 1289 |
| NspI | RCATGY | 1 cut(s) | 1238 |
| NspV | TTCGAA | 1 cut(s) | 666 |
| PciI | ACATGT | 1 cut(s) | 1234 |
| PctI | GAATGC | 3 cut(s) | 172, 563, 1164 |
| PfeI | GAWTC | 2 cut(s) | 787, 1227 |
| PkrI | GCNGC | 8 cut(s) | 68, 255, 280, 283, 801, 804, 880, 892 |
| PleI | GAGTC | 1 cut(s) | 194 |
| PpsI | GAGTC | 1 cut(s) | 194 |
| PpuMI | RGGWCCY | 1 cut(s) | 32 |
| PscI | ACATGT | 1 cut(s) | 1234 |
| PshBI | ATTAAT | 2 cut(s) | 393, 849 |
| Psp5II | RGGWCCY | 1 cut(s) | 32 |
| PspN4I | GGNNCC | 2 cut(s) | 705, 877 |
| PspPI | GGNCC | 2 cut(s) | 32, 657 |
| PspPPI | RGGWCCY | 1 cut(s) | 32 |
| PstI | CTGCAG | 1 cut(s) | 40 |
| PstNI | CAGNNNCTG | 2 cut(s) | 185, 1178 |
| RsaI | GTAC | 2 cut(s) | 367, 631 |
| RsaNI | GTAC | 2 cut(s) | 366, 630 |
| RseI | CAYNNNNRTG | 1 cut(s) | 179 |
| SacII | CCGCGG | 1 cut(s) | 282 |
| SaqAI | TTAA | 3 cut(s) | 393, 474, 849 |
| SatI | GCNGC | 8 cut(s) | 67, 254, 279, 282, 800, 803, 879, 891 |
| Sau3AI | GATC | 1 cut(s) | 1275 |
| Sau96I | GGNCC | 2 cut(s) | 32, 657 |
| SchI | GAGTC | 1 cut(s) | 194 |
| ScrFI | CCNGG | 2 cut(s) | 918, 919 |
| SfaNI | GCATC | 4 cut(s) | 114, 718, 1107, 1207 |
| SfcI | CTRYAG | 2 cut(s) | 36, 864 |
| Sfr303I | CCGCGG | 1 cut(s) | 282 |
| SfuI | TTCGAA | 1 cut(s) | 666 |
| SgrBI | CCGCGG | 1 cut(s) | 282 |
| SinI | GGWCC | 1 cut(s) | 32 |
| SmaI | CCCGGG | 1 cut(s) | 919 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 179 |
| Sse9I | AATT | 8 cut(s) | 285, 526, 602, 662, 1032, 1148, 1155, 1193 |
| SsiI | CCGC | 7 cut(s) | 64, 69, 279, 281, 360, 428, 879 |
| SspMI | CTAG | 2 cut(s) | 312, 783 |
| StyD4I | CCNGG | 2 cut(s) | 916, 917 |
| StyI | CCWWGG | 2 cut(s) | 148, 792 |
| TaaI | ACNGT | 7 cut(s) | 84, 160, 181, 340, 482, 979, 1289 |
| TaiI | ACGT | 2 cut(s) | 211, 762 |
| TaqI | TCGA | 4 cut(s) | 27, 459, 666, 1028 |
| TasI | AATT | 8 cut(s) | 285, 526, 602, 662, 1032, 1148, 1155, 1193 |
| TauI | GCSGC | 3 cut(s) | 281, 284, 881 |
| TfiI | GAWTC | 2 cut(s) | 787, 1227 |
| Tru1I | TTAA | 3 cut(s) | 393, 474, 849 |
| Tru9I | TTAA | 3 cut(s) | 393, 474, 849 |
| TscAI | CASTG | 3 cut(s) | 186, 345, 1264 |
| TseFI | GTSAC | 3 cut(s) | 949, 960, 1289 |
| TseI | GCWGC | 5 cut(s) | 66, 253, 799, 802, 890 |
| Tsp45I | GTSAC | 3 cut(s) | 949, 960, 1289 |
| TspDTI | ATGAA | 4 cut(s) | 17, 570, 925, 1003 |
| TspMI | CCCGGG | 1 cut(s) | 917 |
| TspRI | CASTG | 3 cut(s) | 186, 345, 1264 |
| VpaK11BI | GGWCC | 1 cut(s) | 32 |
| VspI | ATTAAT | 2 cut(s) | 393, 849 |
| XapI | RAATTY | 5 cut(s) | 526, 602, 662, 1148, 1193 |
| XceI | RCATGY | 1 cut(s) | 1238 |
| XmaI | CCCGGG | 1 cut(s) | 917 |
| XspI | CTAG | 2 cut(s) | 312, 783 |