Heat shock cognate 70 kDa
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AarI | CACCTGC | 1 cut(s) | 346 |
| Acc36I | ACCTGC | 1 cut(s) | 346 |
| AccB1I | GGYRCC | 1 cut(s) | 751 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 341 |
| AccI | GTMKAC | 1 cut(s) | 173 |
| AciI | CCGC | 1 cut(s) | 755 |
| AclWI | GGATC | 1 cut(s) | 592 |
| AcuI | CTGAAG | 2 cut(s) | 369, 549 |
| AfiI | CCNNNNNNNGG | 3 cut(s) | 224, 341, 581 |
| AgsI | TTSAA | 5 cut(s) | 47, 71, 515, 876, 918 |
| AjnI | CCWGG | 4 cut(s) | 81, 319, 438, 802 |
| AluBI | AGCT | 5 cut(s) | 224, 366, 458, 761, 810 |
| AluI | AGCT | 5 cut(s) | 224, 366, 458, 761, 810 |
| AlwI | GGATC | 1 cut(s) | 592 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 122 |
| Ama87I | CYCGRG | 1 cut(s) | 811 |
| AoxI | GGCC | 3 cut(s) | 254, 293, 936 |
| ApeKI | GCWGC | 2 cut(s) | 122, 761 |
| AspA2I | CCTAGG | 1 cut(s) | 851 |
| AspS9I | GGNCC | 3 cut(s) | 79, 85, 309 |
| AsuC2I | CCSGG | 2 cut(s) | 288, 912 |
| AsuHPI | GGTGA | 5 cut(s) | 122, 335, 658, 719, 852 |
| AvaI | CYCGRG | 1 cut(s) | 811 |
| AvaII | GGWCC | 3 cut(s) | 79, 85, 309 |
| AvrII | CCTAGG | 1 cut(s) | 851 |
| BaeGI | GKGCMC | 1 cut(s) | 411 |
| BanI | GGYRCC | 1 cut(s) | 751 |
| BanII | GRGCYC | 1 cut(s) | 149 |
| BbsI | GAAGAC | 1 cut(s) | 54 |
| BbvI | GCAGC | 2 cut(s) | 109, 748 |
| BccI | CCATC | 4 cut(s) | 101, 135, 517, 707 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 879, 913 |
| BciT130I | CCWGG | 4 cut(s) | 83, 321, 440, 804 |
| BcnI | CCSGG | 2 cut(s) | 288, 912 |
| BfaI | CTAG | 1 cut(s) | 852 |
| BfuAI | ACCTGC | 1 cut(s) | 346 |
| BglII | AGATCT | 1 cut(s) | 49 |
| BisI | GCNGC | 3 cut(s) | 123, 755, 762 |
| BlnI | CCTAGG | 1 cut(s) | 851 |
| BlsI | GCNGC | 3 cut(s) | 124, 756, 763 |
| Bme1390I | CCNGG | 6 cut(s) | 83, 288, 321, 440, 804, 912 |
| Bme18I | GGWCC | 3 cut(s) | 79, 85, 309 |
| BmeT110I | CYCGRG | 1 cut(s) | 811 |
| BmgT120I | GGNCC | 3 cut(s) | 79, 85, 309 |
| BmiI | GGNNCC | 4 cut(s) | 87, 146, 330, 753 |
| BmrFI | CCNGG | 6 cut(s) | 83, 288, 321, 440, 804, 912 |
| BmrI | ACTGGG | 1 cut(s) | 83 |
| BmsI | GCATC | 4 cut(s) | 103, 632, 899, 905 |
| BmuI | ACTGGG | 1 cut(s) | 83 |
| BpiI | GAAGAC | 1 cut(s) | 54 |
| BpuEI | CTTGAG | 2 cut(s) | 212, 603 |
| BpuMI | CCSGG | 2 cut(s) | 288, 912 |
| BsaJI | CCNNGG | 2 cut(s) | 776, 851 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 912, 942 |
| Bsc4I | CCNNNNNNNGG | 3 cut(s) | 224, 341, 581 |
| Bse1I | ACTGG | 2 cut(s) | 89, 257 |
| Bse3DI | GCAATG | 1 cut(s) | 751 |
| BseBI | CCWGG | 4 cut(s) | 83, 321, 440, 804 |
| BseDI | CCNNGG | 2 cut(s) | 776, 851 |
| BseGI | GGATG | 2 cut(s) | 10, 146 |
| BseLI | CCNNNNNNNGG | 3 cut(s) | 224, 341, 581 |
| BseMI | GCAATG | 1 cut(s) | 751 |
| BseMII | CTCAG | 1 cut(s) | 480 |
| BseNI | ACTGG | 2 cut(s) | 89, 257 |
| BseSI | GKGCMC | 1 cut(s) | 411 |
| BseXI | GCAGC | 2 cut(s) | 109, 748 |
| BseYI | CCCAGC | 1 cut(s) | 341 |
| BsgI | GTGCAG | 1 cut(s) | 747 |
| BshFI | GGCC | 3 cut(s) | 256, 295, 938 |
| BshNI | GGYRCC | 1 cut(s) | 751 |
| BsiHKCI | CYCGRG | 1 cut(s) | 811 |
| BsiSI | CCGG | 2 cut(s) | 287, 911 |
| BslFI | GGGAC | 1 cut(s) | 358 |
| BslI | CCNNNNNNNGG | 3 cut(s) | 224, 341, 581 |
| BsmFI | GGGAC | 1 cut(s) | 358 |
| BsnI | GGCC | 3 cut(s) | 256, 295, 938 |
| BsoBI | CYCGRG | 1 cut(s) | 811 |
| Bsp1286I | GDGCHC | 2 cut(s) | 149, 411 |
| Bsp143I | GATC | 4 cut(s) | 16, 49, 584, 788 |
| BspACI | CCGC | 1 cut(s) | 755 |
| BspANI | GGCC | 3 cut(s) | 256, 295, 938 |
| BspCNI | CTCAG | 1 cut(s) | 481 |
| BspLI | GGNNCC | 4 cut(s) | 87, 146, 330, 753 |
| BspMI | ACCTGC | 1 cut(s) | 346 |
| BspPI | GGATC | 1 cut(s) | 592 |
| BspQI | GCTCTTC | 1 cut(s) | 356 |
| BspT107I | GGYRCC | 1 cut(s) | 751 |
| BsrDI | GCAATG | 1 cut(s) | 751 |
| BsrI | ACTGG | 2 cut(s) | 89, 257 |
| BssECI | CCNNGG | 2 cut(s) | 776, 851 |
| BssMI | GATC | 4 cut(s) | 16, 49, 584, 788 |
| BssNAI | GTATAC | 1 cut(s) | 174 |
| BssT1I | CCWWGG | 2 cut(s) | 776, 851 |
| Bst1107I | GTATAC | 1 cut(s) | 174 |
| Bst2UI | CCWGG | 4 cut(s) | 83, 321, 440, 804 |
| Bst4CI | ACNGT | 3 cut(s) | 273, 694, 904 |
| Bst6I | CTCTTC | 1 cut(s) | 356 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 122 |
| BstC8I | GCNNGC | 1 cut(s) | 759 |
| BstDEI | CTNAG | 1 cut(s) | 489 |
| BstF5I | GGATG | 2 cut(s) | 10, 146 |
| BstKTI | GATC | 4 cut(s) | 19, 52, 587, 791 |
| BstMBI | GATC | 4 cut(s) | 16, 49, 584, 788 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 122, 230 |
| BstNI | CCWGG | 4 cut(s) | 83, 321, 440, 804 |
| BstNSI | RCATGY | 1 cut(s) | 611 |
| BstSCI | CCNGG | 6 cut(s) | 81, 286, 319, 438, 802, 910 |
| BstSLI | GKGCMC | 1 cut(s) | 411 |
| BstV1I | GCAGC | 2 cut(s) | 109, 748 |
| BstV2I | GAAGAC | 1 cut(s) | 54 |
| BstX2I | RGATCY | 1 cut(s) | 49 |
| BstYI | RGATCY | 1 cut(s) | 49 |
| BstZ17I | GTATAC | 1 cut(s) | 174 |
| BsuRI | GGCC | 3 cut(s) | 256, 295, 938 |
| BtsCI | GGATG | 2 cut(s) | 10, 146 |
| BtsIMutI | CAGTG | 1 cut(s) | 269 |
| BveI | ACCTGC | 1 cut(s) | 346 |
| Cac8I | GCNNGC | 1 cut(s) | 759 |
| CaiI | CAGNNNCTG | 1 cut(s) | 122 |
| Cfr13I | GGNCC | 3 cut(s) | 79, 85, 309 |
| CsiI | ACCWGGT | 1 cut(s) | 319 |
| CviAII | CATG | 3 cut(s) | 608, 643, 799 |
| DdeI | CTNAG | 1 cut(s) | 489 |
| DpnI | GATC | 4 cut(s) | 18, 51, 586, 790 |
| DpnII | GATC | 4 cut(s) | 16, 49, 584, 788 |
| DraI | TTTAAA | 1 cut(s) | 213 |
| Eam1104I | CTCTTC | 1 cut(s) | 356 |
| EarI | CTCTTC | 1 cut(s) | 356 |
| Eco130I | CCWWGG | 2 cut(s) | 776, 851 |
| Eco24I | GRGCYC | 1 cut(s) | 149 |
| Eco47I | GGWCC | 3 cut(s) | 79, 85, 309 |
| Eco57I | CTGAAG | 2 cut(s) | 369, 549 |
| Eco88I | CYCGRG | 1 cut(s) | 811 |
| EcoO109I | RGGNCCY | 1 cut(s) | 79 |
| EcoRII | CCWGG | 4 cut(s) | 81, 319, 438, 802 |
| EcoT14I | CCWWGG | 2 cut(s) | 776, 851 |
| EcoT38I | GRGCYC | 1 cut(s) | 149 |
| ErhI | CCWWGG | 2 cut(s) | 776, 851 |
| FaeI | CATG | 3 cut(s) | 611, 646, 802 |
| FaqI | GGGAC | 1 cut(s) | 358 |
| FatI | CATG | 3 cut(s) | 607, 642, 798 |
| FauNDI | CATATG | 1 cut(s) | 199 |
| FblI | GTMKAC | 1 cut(s) | 173 |
| Fnu4HI | GCNGC | 3 cut(s) | 123, 755, 762 |
| FokI | GGATG | 2 cut(s) | 17, 153 |
| FriOI | GRGCYC | 1 cut(s) | 149 |
| Fsp4HI | GCNGC | 3 cut(s) | 123, 755, 762 |
| FspBI | CTAG | 1 cut(s) | 852 |
| GluI | GCNGC | 3 cut(s) | 123, 755, 762 |
| GsaI | CCCAGC | 1 cut(s) | 345 |
| HaeIII | GGCC | 3 cut(s) | 256, 295, 938 |
| HapII | CCGG | 2 cut(s) | 287, 911 |
| Hin1II | CATG | 3 cut(s) | 611, 646, 802 |
| HinfI | GANTC | 1 cut(s) | 822 |
| HpaII | CCGG | 2 cut(s) | 287, 911 |
| HphI | GGTGA | 5 cut(s) | 122, 335, 658, 719, 852 |
| Hpy166II | GTNNAC | 2 cut(s) | 174, 448 |
| Hpy188I | TCNGA | 2 cut(s) | 490, 660 |
| Hpy188III | TCNNGA | 4 cut(s) | 191, 557, 813, 826 |
| Hpy8I | GTNNAC | 2 cut(s) | 174, 448 |
| HpyAV | CCTTC | 2 cut(s) | 285, 575 |
| HpyCH4III | ACNGT | 3 cut(s) | 273, 694, 904 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 122, 230 |
| HpyF3I | CTNAG | 1 cut(s) | 489 |
| Hsp92II | CATG | 3 cut(s) | 611, 646, 802 |
| Kzo9I | GATC | 4 cut(s) | 16, 49, 584, 788 |
| LguI | GCTCTTC | 1 cut(s) | 356 |
| LmnI | GCTCC | 1 cut(s) | 144 |
| Lsp1109I | GCAGC | 2 cut(s) | 109, 748 |
| LweI | GCATC | 4 cut(s) | 103, 632, 899, 905 |
| MabI | ACCWGGT | 1 cut(s) | 319 |
| MaeI | CTAG | 1 cut(s) | 852 |
| MaeIII | GTNAC | 1 cut(s) | 688 |
| MalI | GATC | 4 cut(s) | 18, 51, 586, 790 |
| MboI | GATC | 4 cut(s) | 16, 49, 584, 788 |
| MboII | GAAGA | 7 cut(s) | 20, 54, 59, 373, 402, 444, 542 |
| MflI | RGATCY | 1 cut(s) | 49 |
| MhlI | GDGCHC | 2 cut(s) | 149, 411 |
| MluCI | AATT | 3 cut(s) | 378, 468, 589 |
| MmeI | TCCRAC | 1 cut(s) | 73 |
| MseI | TTAA | 3 cut(s) | 212, 381, 428 |
| MspA1I | CMGCKG | 1 cut(s) | 761 |
| MspI | CCGG | 2 cut(s) | 287, 911 |
| MspR9I | CCNGG | 6 cut(s) | 83, 288, 321, 440, 804, 912 |
| MvaI | CCWGG | 4 cut(s) | 83, 321, 440, 804 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 122, 230 |
| NciI | CCSGG | 2 cut(s) | 288, 912 |
| NdeI | CATATG | 1 cut(s) | 199 |
| NdeII | GATC | 4 cut(s) | 16, 49, 584, 788 |
| NlaIII | CATG | 3 cut(s) | 611, 646, 802 |
| NlaIV | GGNNCC | 4 cut(s) | 87, 146, 330, 753 |
| NmuCI | GTSAC | 1 cut(s) | 688 |
| NspI | RCATGY | 1 cut(s) | 611 |
| PaqCI | CACCTGC | 1 cut(s) | 346 |
| PciSI | GCTCTTC | 1 cut(s) | 356 |
| PfeI | GAWTC | 1 cut(s) | 822 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 341 |
| PkrI | GCNGC | 3 cut(s) | 124, 756, 763 |
| PpuMI | RGGWCCY | 1 cut(s) | 79 |
| Psp5II | RGGWCCY | 1 cut(s) | 79 |
| Psp6I | CCWGG | 4 cut(s) | 81, 319, 438, 802 |
| PspFI | CCCAGC | 1 cut(s) | 341 |
| PspGI | CCWGG | 4 cut(s) | 81, 319, 438, 802 |
| PspN4I | GGNNCC | 4 cut(s) | 87, 146, 330, 753 |
| PspPI | GGNCC | 3 cut(s) | 79, 85, 309 |
| PspPPI | RGGWCCY | 1 cut(s) | 79 |
| PstNI | CAGNNNCTG | 1 cut(s) | 122 |
| PsuI | RGATCY | 1 cut(s) | 49 |
| PvuII | CAGCTG | 1 cut(s) | 761 |
| SapI | GCTCTTC | 1 cut(s) | 356 |
| SaqAI | TTAA | 3 cut(s) | 212, 381, 428 |
| SatI | GCNGC | 3 cut(s) | 123, 755, 762 |
| Sau3AI | GATC | 4 cut(s) | 16, 49, 584, 788 |
| Sau96I | GGNCC | 3 cut(s) | 79, 85, 309 |
| ScrFI | CCNGG | 6 cut(s) | 83, 288, 321, 440, 804, 912 |
| SduI | GDGCHC | 2 cut(s) | 149, 411 |
| SexAI | ACCWGGT | 1 cut(s) | 319 |
| SfaNI | GCATC | 4 cut(s) | 103, 632, 899, 905 |
| SinI | GGWCC | 3 cut(s) | 79, 85, 309 |
| SmlI | CTYRAG | 2 cut(s) | 191, 618 |
| SmoI | CTYRAG | 2 cut(s) | 191, 618 |
| Sse9I | AATT | 3 cut(s) | 378, 468, 589 |
| SsiI | CCGC | 1 cut(s) | 755 |
| SspMI | CTAG | 1 cut(s) | 852 |
| StyD4I | CCNGG | 6 cut(s) | 81, 286, 319, 438, 802, 910 |
| StyI | CCWWGG | 2 cut(s) | 776, 851 |
| TaaI | ACNGT | 3 cut(s) | 273, 694, 904 |
| TaqI | TCGA | 2 cut(s) | 15, 899 |
| TasI | AATT | 3 cut(s) | 378, 468, 589 |
| TauI | GCSGC | 1 cut(s) | 757 |
| TfiI | GAWTC | 1 cut(s) | 822 |
| Tru1I | TTAA | 3 cut(s) | 212, 381, 428 |
| Tru9I | TTAA | 3 cut(s) | 212, 381, 428 |
| TscAI | CASTG | 1 cut(s) | 276 |
| TseFI | GTSAC | 1 cut(s) | 688 |
| TseI | GCWGC | 2 cut(s) | 122, 761 |
| Tsp45I | GTSAC | 1 cut(s) | 688 |
| TspDTI | ATGAA | 3 cut(s) | 21, 31, 594 |
| TspRI | CASTG | 1 cut(s) | 276 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 341 |
| VpaK11BI | GGWCC | 3 cut(s) | 79, 85, 309 |
| XceI | RCATGY | 1 cut(s) | 611 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 931 |
| XmaJI | CCTAGG | 1 cut(s) | 851 |
| XmiI | GTMKAC | 1 cut(s) | 173 |
| XspI | CTAG | 1 cut(s) | 852 |