Belongs to the heat shock protein 70 family
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 518 |
| AatII | GACGTC | 1 cut(s) | 1251 |
| AccBSI | CCGCTC | 1 cut(s) | 110 |
| AciI | CCGC | 3 cut(s) | 108, 672, 768 |
| AclWI | GGATC | 2 cut(s) | 415, 876 |
| AcoI | YGGCCR | 2 cut(s) | 266, 666 |
| AcsI | RAATTY | 4 cut(s) | 908, 928, 1032, 1478 |
| AcuI | CTGAAG | 3 cut(s) | 369, 414, 1596 |
| AcyI | GRCGYC | 2 cut(s) | 651, 1248 |
| AfaI | GTAC | 3 cut(s) | 369, 616, 1385 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 1626 |
| AjuI | GAANNNNNNNTTGG | 4 cut(s) | 387, 419, 740, 772 |
| AloI | GAACNNNNNNTCC | 2 cut(s) | 1113, 1145 |
| AluBI | AGCT | 7 cut(s) | 262, 782, 1051, 1127, 1136, 1486, 1505 |
| AluI | AGCT | 7 cut(s) | 262, 782, 1051, 1127, 1136, 1486, 1505 |
| Alw21I | GWGCWC | 2 cut(s) | 784, 1488 |
| Alw26I | GTCTC | 3 cut(s) | 672, 817, 1573 |
| AlwI | GGATC | 2 cut(s) | 415, 876 |
| Ama87I | CYCGRG | 2 cut(s) | 775, 900 |
| AoxI | GGCC | 5 cut(s) | 266, 325, 496, 550, 666 |
| ApeKI | GCWGC | 8 cut(s) | 61, 158, 344, 482, 533, 536, 1124, 1136 |
| ApoI | RAATTY | 4 cut(s) | 908, 928, 1032, 1478 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 932 |
| AspS9I | GGNCC | 2 cut(s) | 325, 1629 |
| AsuHPI | GGTGA | 6 cut(s) | 656, 677, 707, 730, 1451, 1487 |
| AvaI | CYCGRG | 2 cut(s) | 775, 900 |
| AvaII | GGWCC | 1 cut(s) | 1629 |
| BalI | TGGCCA | 2 cut(s) | 268, 668 |
| BanII | GRGCYC | 2 cut(s) | 784, 1488 |
| BbsI | GAAGAC | 1 cut(s) | 705 |
| Bbv12I | GWGCWC | 2 cut(s) | 784, 1488 |
| BbvI | GCAGC | 8 cut(s) | 73, 170, 331, 469, 520, 548, 1123, 1136 |
| BccI | CCATC | 3 cut(s) | 121, 641, 658 |
| BceAI | ACGGC | 1 cut(s) | 565 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 50, 84 |
| BciVI | GTATCC | 1 cut(s) | 1568 |
| BclI | TGATCA | 3 cut(s) | 1294, 1300, 1422 |
| BcoDI | GTCTC | 3 cut(s) | 672, 817, 1573 |
| BfmI | CTRYAG | 3 cut(s) | 534, 618, 1137 |
| BfuI | GTATCC | 1 cut(s) | 1568 |
| BisI | GCNGC | 8 cut(s) | 62, 159, 345, 483, 534, 537, 1125, 1137 |
| BlsI | GCNGC | 8 cut(s) | 63, 160, 346, 484, 535, 538, 1126, 1138 |
| BmcAI | AGTACT | 1 cut(s) | 1385 |
| Bme18I | GGWCC | 1 cut(s) | 1629 |
| BmeT110I | CYCGRG | 2 cut(s) | 775, 900 |
| BmgT120I | GGNCC | 2 cut(s) | 325, 1629 |
| BmiI | GGNNCC | 1 cut(s) | 951 |
| BmsI | GCATC | 8 cut(s) | 145, 193, 226, 369, 964, 1101, 1174, 1537 |
| BpiI | GAAGAC | 1 cut(s) | 705 |
| BpmI | CTGGAG | 2 cut(s) | 696, 1485 |
| Bsa29I | ATCGAT | 1 cut(s) | 858 |
| BsaBI | GATNNNNATC | 2 cut(s) | 1299, 1305 |
| BsaHI | GRCGYC | 2 cut(s) | 651, 1248 |
| BsaJI | CCNNGG | 2 cut(s) | 363, 688 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 1113, 1143 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 1626 |
| Bse118I | RCCGGY | 1 cut(s) | 566 |
| Bse1I | ACTGG | 2 cut(s) | 639, 1594 |
| Bse3DI | GCAATG | 1 cut(s) | 540 |
| Bse8I | GATNNNNATC | 2 cut(s) | 1299, 1305 |
| BseCI | ATCGAT | 1 cut(s) | 858 |
| BseDI | CCNNGG | 2 cut(s) | 363, 688 |
| BseGI | GGATG | 3 cut(s) | 359, 1006, 1066 |
| BseJI | GATNNNNATC | 2 cut(s) | 1299, 1305 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 1626 |
| BseMI | GCAATG | 1 cut(s) | 540 |
| BseNI | ACTGG | 2 cut(s) | 639, 1594 |
| BseRI | GAGGAG | 1 cut(s) | 835 |
| BseXI | GCAGC | 8 cut(s) | 73, 170, 331, 469, 520, 548, 1123, 1136 |
| BseYI | CCCAGC | 1 cut(s) | 989 |
| BshFI | GGCC | 5 cut(s) | 268, 327, 498, 552, 668 |
| BshVI | ATCGAT | 1 cut(s) | 858 |
| BsiHKAI | GWGCWC | 2 cut(s) | 784, 1488 |
| BsiHKCI | CYCGRG | 2 cut(s) | 775, 900 |
| BsiSI | CCGG | 1 cut(s) | 567 |
| BslFI | GGGAC | 2 cut(s) | 46, 422 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 1626 |
| BsmAI | GTCTC | 3 cut(s) | 672, 817, 1573 |
| BsmBI | CGTCTC | 1 cut(s) | 1573 |
| BsmFI | GGGAC | 2 cut(s) | 46, 422 |
| BsnI | GGCC | 5 cut(s) | 268, 327, 498, 552, 668 |
| BsoBI | CYCGRG | 2 cut(s) | 775, 900 |
| Bsp1286I | GDGCHC | 2 cut(s) | 784, 1488 |
| Bsp143I | GATC | 9 cut(s) | 67, 91, 171, 407, 868, 1294, 1300, 1312, 1422 |
| Bsp19I | CCATGG | 1 cut(s) | 363 |
| BspACI | CCGC | 3 cut(s) | 108, 672, 768 |
| BspANI | GGCC | 5 cut(s) | 268, 327, 498, 552, 668 |
| BspDI | ATCGAT | 1 cut(s) | 858 |
| BspHI | TCATGA | 2 cut(s) | 1297, 1309 |
| BspLI | GGNNCC | 1 cut(s) | 951 |
| BspMAI | CTGCAG | 2 cut(s) | 538, 1141 |
| BspPI | GGATC | 2 cut(s) | 415, 876 |
| BspQI | GCTCTTC | 1 cut(s) | 1030 |
| BsrBI | CCGCTC | 1 cut(s) | 110 |
| BsrDI | GCAATG | 1 cut(s) | 540 |
| BsrFI | RCCGGY | 1 cut(s) | 566 |
| BsrI | ACTGG | 2 cut(s) | 639, 1594 |
| BssAI | RCCGGY | 1 cut(s) | 566 |
| BssECI | CCNNGG | 2 cut(s) | 363, 688 |
| BssMI | GATC | 9 cut(s) | 67, 91, 171, 407, 868, 1294, 1300, 1312, 1422 |
| BssNI | GRCGYC | 2 cut(s) | 651, 1248 |
| BssT1I | CCWWGG | 2 cut(s) | 363, 688 |
| Bst4CI | ACNGT | 6 cut(s) | 196, 436, 589, 728, 828, 1556 |
| Bst6I | CTCTTC | 1 cut(s) | 1030 |
| BstACI | GRCGYC | 2 cut(s) | 651, 1248 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 542 |
| BstC8I | GCNNGC | 2 cut(s) | 496, 979 |
| BstDEI | CTNAG | 1 cut(s) | 138 |
| BstDSI | CCRYGG | 1 cut(s) | 363 |
| BstF5I | GGATG | 3 cut(s) | 359, 1006, 1066 |
| BstKTI | GATC | 9 cut(s) | 70, 94, 174, 410, 871, 1297, 1303, 1315, 1425 |
| BstMAI | GTCTC | 3 cut(s) | 672, 817, 1573 |
| BstMBI | GATC | 9 cut(s) | 67, 91, 171, 407, 868, 1294, 1300, 1312, 1422 |
| BstMWI | GCNNNNNNNGC | 4 cut(s) | 491, 542, 1121, 1133 |
| BstNSI | RCATGY | 3 cut(s) | 243, 807, 1420 |
| BstSFI | CTRYAG | 3 cut(s) | 534, 618, 1137 |
| BstV1I | GCAGC | 8 cut(s) | 73, 170, 331, 469, 520, 548, 1123, 1136 |
| BstV2I | GAAGAC | 1 cut(s) | 705 |
| Bsu15I | ATCGAT | 1 cut(s) | 858 |
| BsuI | GTATCC | 1 cut(s) | 1568 |
| BsuRI | GGCC | 5 cut(s) | 268, 327, 498, 552, 668 |
| BsuTUI | ATCGAT | 1 cut(s) | 858 |
| BtgI | CCRYGG | 1 cut(s) | 363 |
| BtsCI | GGATG | 3 cut(s) | 359, 1006, 1066 |
| BtsI | GCAGTG | 1 cut(s) | 60 |
| BtsIMutI | CAGTG | 4 cut(s) | 60, 129, 1432, 1587 |
| Cac8I | GCNNGC | 2 cut(s) | 496, 979 |
| CciI | TCATGA | 2 cut(s) | 1297, 1309 |
| Cfr10I | RCCGGY | 1 cut(s) | 566 |
| Cfr13I | GGNCC | 2 cut(s) | 325, 1629 |
| ClaI | ATCGAT | 1 cut(s) | 858 |
| CseI | GACGC | 2 cut(s) | 640, 995 |
| Csp6I | GTAC | 3 cut(s) | 368, 615, 1384 |
| CviQI | GTAC | 3 cut(s) | 368, 615, 1384 |
| DdeI | CTNAG | 1 cut(s) | 138 |
| DpnI | GATC | 9 cut(s) | 69, 93, 173, 409, 870, 1296, 1302, 1314, 1424 |
| DpnII | GATC | 9 cut(s) | 67, 91, 171, 407, 868, 1294, 1300, 1312, 1422 |
| EaeI | YGGCCR | 2 cut(s) | 266, 666 |
| Eam1104I | CTCTTC | 1 cut(s) | 1030 |
| EarI | CTCTTC | 1 cut(s) | 1030 |
| Ecl136II | GAGCTC | 2 cut(s) | 782, 1486 |
| Eco130I | CCWWGG | 2 cut(s) | 363, 688 |
| Eco24I | GRGCYC | 2 cut(s) | 784, 1488 |
| Eco47I | GGWCC | 1 cut(s) | 1629 |
| Eco53kI | GAGCTC | 2 cut(s) | 782, 1486 |
| Eco57I | CTGAAG | 3 cut(s) | 369, 414, 1596 |
| Eco88I | CYCGRG | 2 cut(s) | 775, 900 |
| EcoICRI | GAGCTC | 2 cut(s) | 782, 1486 |
| EcoO109I | RGGNCCY | 1 cut(s) | 1629 |
| EcoRI | GAATTC | 1 cut(s) | 1032 |
| EcoT14I | CCWWGG | 2 cut(s) | 363, 688 |
| EcoT22I | ATGCAT | 3 cut(s) | 241, 384, 805 |
| EcoT38I | GRGCYC | 2 cut(s) | 784, 1488 |
| ErhI | CCWWGG | 2 cut(s) | 363, 688 |
| Esp3I | CGTCTC | 1 cut(s) | 1573 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 1439, 1471 |
| FaqI | GGGAC | 2 cut(s) | 46, 422 |
| FauI | CCCGC | 1 cut(s) | 761 |
| FauNDI | CATATG | 1 cut(s) | 1117 |
| FbaI | TGATCA | 3 cut(s) | 1294, 1300, 1422 |
| Fnu4HI | GCNGC | 8 cut(s) | 62, 159, 345, 483, 534, 537, 1125, 1137 |
| FokI | GGATG | 3 cut(s) | 346, 1013, 1073 |
| FriOI | GRGCYC | 2 cut(s) | 784, 1488 |
| Fsp4HI | GCNGC | 8 cut(s) | 62, 159, 345, 483, 534, 537, 1125, 1137 |
| GluI | GCNGC | 8 cut(s) | 62, 159, 345, 483, 534, 537, 1125, 1137 |
| GsaI | CCCAGC | 1 cut(s) | 993 |
| GsuI | CTGGAG | 2 cut(s) | 696, 1485 |
| HaeIII | GGCC | 5 cut(s) | 268, 327, 498, 552, 668 |
| HapII | CCGG | 1 cut(s) | 567 |
| HgaI | GACGC | 2 cut(s) | 640, 995 |
| Hin1I | GRCGYC | 2 cut(s) | 651, 1248 |
| HincII | GTYRAC | 1 cut(s) | 847 |
| HindII | GTYRAC | 1 cut(s) | 847 |
| HindIII | AAGCTT | 1 cut(s) | 260 |
| HinfI | GANTC | 2 cut(s) | 508, 1345 |
| HpaII | CCGG | 1 cut(s) | 567 |
| HphI | GGTGA | 6 cut(s) | 656, 677, 707, 730, 1451, 1487 |
| Hpy166II | GTNNAC | 5 cut(s) | 88, 458, 721, 847, 1587 |
| Hpy188I | TCNGA | 4 cut(s) | 574, 1038, 1246, 1402 |
| Hpy8I | GTNNAC | 5 cut(s) | 88, 458, 721, 847, 1587 |
| Hpy99I | CGWCG | 1 cut(s) | 1556 |
| HpyAV | CCTTC | 7 cut(s) | 136, 278, 805, 1033, 1069, 1378, 1430 |
| HpyCH4III | ACNGT | 6 cut(s) | 196, 436, 589, 728, 828, 1556 |
| HpyCH4IV | ACGT | 4 cut(s) | 463, 762, 1248, 1566 |
| HpyF10VI | GCNNNNNNNGC | 4 cut(s) | 491, 542, 1121, 1133 |
| HpyF3I | CTNAG | 1 cut(s) | 138 |
| HpySE526I | ACGT | 4 cut(s) | 463, 762, 1248, 1566 |
| Hsp92I | GRCGYC | 2 cut(s) | 651, 1248 |
| Ksp22I | TGATCA | 3 cut(s) | 1294, 1300, 1422 |
| Kzo9I | GATC | 9 cut(s) | 67, 91, 171, 407, 868, 1294, 1300, 1312, 1422 |
| LguI | GCTCTTC | 1 cut(s) | 1030 |
| LmnI | GCTCC | 4 cut(s) | 115, 949, 1121, 1510 |
| Lsp1109I | GCAGC | 8 cut(s) | 73, 170, 331, 469, 520, 548, 1123, 1136 |
| LweI | GCATC | 8 cut(s) | 145, 193, 226, 369, 964, 1101, 1174, 1537 |
| MaeII | ACGT | 4 cut(s) | 463, 762, 1248, 1566 |
| MaeIII | GTNAC | 1 cut(s) | 1249 |
| MalI | GATC | 9 cut(s) | 69, 93, 173, 409, 870, 1296, 1302, 1314, 1424 |
| MbiI | CCGCTC | 1 cut(s) | 110 |
| MboI | GATC | 9 cut(s) | 67, 91, 171, 407, 868, 1294, 1300, 1312, 1422 |
| MboII | GAAGA | 6 cut(s) | 237, 362, 710, 925, 1017, 1307 |
| MfeI | CAATTG | 1 cut(s) | 917 |
| MhlI | GDGCHC | 2 cut(s) | 784, 1488 |
| MlsI | TGGCCA | 2 cut(s) | 268, 668 |
| MluCI | AATT | 7 cut(s) | 737, 908, 917, 928, 1032, 1079, 1478 |
| MluNI | TGGCCA | 2 cut(s) | 268, 668 |
| MnlI | CCTC | 9 cut(s) | 563, 686, 738, 783, 813, 1099, 1509, 1571, 1595 |
| Mox20I | TGGCCA | 2 cut(s) | 268, 668 |
| Mph1103I | ATGCAT | 3 cut(s) | 241, 384, 805 |
| MroXI | GAANNNNTTC | 1 cut(s) | 932 |
| MscI | TGGCCA | 2 cut(s) | 268, 668 |
| MseI | TTAA | 6 cut(s) | 165, 450, 1145, 1193, 1227, 1539 |
| MslI | CAYNNNNRTG | 1 cut(s) | 1308 |
| Msp20I | TGGCCA | 2 cut(s) | 268, 668 |
| MspA1I | CMGCKG | 2 cut(s) | 674, 1127 |
| MspI | CCGG | 1 cut(s) | 567 |
| MunI | CAATTG | 1 cut(s) | 917 |
| MwoI | GCNNNNNNNGC | 4 cut(s) | 491, 542, 1121, 1133 |
| NcoI | CCATGG | 1 cut(s) | 363 |
| NdeI | CATATG | 1 cut(s) | 1117 |
| NdeII | GATC | 9 cut(s) | 67, 91, 171, 407, 868, 1294, 1300, 1312, 1422 |
| NlaIV | GGNNCC | 1 cut(s) | 951 |
| NmeAIII | GCCGAG | 1 cut(s) | 757 |
| NmuCI | GTSAC | 1 cut(s) | 1249 |
| NsiI | ATGCAT | 3 cut(s) | 241, 384, 805 |
| NspI | RCATGY | 3 cut(s) | 243, 807, 1420 |
| PaeR7I | CTCGAG | 2 cut(s) | 775, 900 |
| PagI | TCATGA | 2 cut(s) | 1297, 1309 |
| PciSI | GCTCTTC | 1 cut(s) | 1030 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 759 |
| PdmI | GAANNNNTTC | 1 cut(s) | 932 |
| PfeI | GAWTC | 2 cut(s) | 508, 1345 |
| PkrI | GCNGC | 8 cut(s) | 63, 160, 346, 484, 535, 538, 1126, 1138 |
| PpuMI | RGGWCCY | 1 cut(s) | 1629 |
| PsiI | TTATAA | 1 cut(s) | 518 |
| Psp124BI | GAGCTC | 2 cut(s) | 784, 1488 |
| Psp5II | RGGWCCY | 1 cut(s) | 1629 |
| PspFI | CCCAGC | 1 cut(s) | 989 |
| PspN4I | GGNNCC | 1 cut(s) | 951 |
| PspPI | GGNCC | 2 cut(s) | 325, 1629 |
| PspPPI | RGGWCCY | 1 cut(s) | 1629 |
| PspXI | VCTCGAGB | 1 cut(s) | 900 |
| PstI | CTGCAG | 2 cut(s) | 538, 1141 |
| PvuII | CAGCTG | 1 cut(s) | 1127 |
| RsaI | GTAC | 3 cut(s) | 369, 616, 1385 |
| RsaNI | GTAC | 3 cut(s) | 368, 615, 1384 |
| RseI | CAYNNNNRTG | 1 cut(s) | 1308 |
| SacI | GAGCTC | 2 cut(s) | 784, 1488 |
| SapI | GCTCTTC | 1 cut(s) | 1030 |
| SaqAI | TTAA | 6 cut(s) | 165, 450, 1145, 1193, 1227, 1539 |
| SatI | GCNGC | 8 cut(s) | 62, 159, 345, 483, 534, 537, 1125, 1137 |
| Sau3AI | GATC | 9 cut(s) | 67, 91, 171, 407, 868, 1294, 1300, 1312, 1422 |
| Sau96I | GGNCC | 2 cut(s) | 325, 1629 |
| ScaI | AGTACT | 1 cut(s) | 1385 |
| SduI | GDGCHC | 2 cut(s) | 784, 1488 |
| SfaNI | GCATC | 8 cut(s) | 145, 193, 226, 369, 964, 1101, 1174, 1537 |
| SfcI | CTRYAG | 3 cut(s) | 534, 618, 1137 |
| Sfr274I | CTCGAG | 2 cut(s) | 775, 900 |
| SinI | GGWCC | 1 cut(s) | 1629 |
| SlaI | CTCGAG | 2 cut(s) | 775, 900 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 1308 |
| SmlI | CTYRAG | 2 cut(s) | 775, 900 |
| SmoI | CTYRAG | 2 cut(s) | 775, 900 |
| Sse9I | AATT | 7 cut(s) | 737, 908, 917, 928, 1032, 1079, 1478 |
| SsiI | CCGC | 3 cut(s) | 108, 672, 768 |
| SspI | AATATT | 1 cut(s) | 1543 |
| SstI | GAGCTC | 2 cut(s) | 784, 1488 |
| StyI | CCWWGG | 2 cut(s) | 363, 688 |
| TaaI | ACNGT | 6 cut(s) | 196, 436, 589, 728, 828, 1556 |
| TaiI | ACGT | 4 cut(s) | 466, 765, 1251, 1569 |
| TaqI | TCGA | 6 cut(s) | 282, 555, 776, 852, 858, 901 |
| TasI | AATT | 7 cut(s) | 737, 908, 917, 928, 1032, 1079, 1478 |
| TatI | WGTACW | 1 cut(s) | 1383 |
| TfiI | GAWTC | 2 cut(s) | 508, 1345 |
| Tru1I | TTAA | 6 cut(s) | 165, 450, 1145, 1193, 1227, 1539 |
| Tru9I | TTAA | 6 cut(s) | 165, 450, 1145, 1193, 1227, 1539 |
| TscAI | CASTG | 4 cut(s) | 60, 136, 1432, 1594 |
| TseFI | GTSAC | 1 cut(s) | 1249 |
| TseI | GCWGC | 8 cut(s) | 61, 158, 344, 482, 533, 536, 1124, 1136 |
| Tsp45I | GTSAC | 1 cut(s) | 1249 |
| TspDTI | ATGAA | 4 cut(s) | 537, 941, 1277, 1391 |
| TspRI | CASTG | 4 cut(s) | 60, 136, 1432, 1594 |
| VpaK11BI | GGWCC | 1 cut(s) | 1629 |
| XapI | RAATTY | 4 cut(s) | 908, 928, 1032, 1478 |
| XceI | RCATGY | 3 cut(s) | 243, 807, 1420 |
| XhoI | CTCGAG | 2 cut(s) | 775, 900 |
| XmnI | GAANNNNTTC | 1 cut(s) | 932 |
| ZraI | GACGTC | 1 cut(s) | 1249 |
| ZrmI | AGTACT | 1 cut(s) | 1385 |
| Zsp2I | ATGCAT | 3 cut(s) | 241, 384, 805 |