Heat shock cognate 70 kDa
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AasI | GACNNNNNNGTC | 2 cut(s) | 124, 254 |
| AccB1I | GGYRCC | 1 cut(s) | 1133 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 1755 |
| AccBSI | CCGCTC | 1 cut(s) | 1161 |
| AccI | GTMKAC | 2 cut(s) | 891, 1344 |
| AccII | CGCG | 2 cut(s) | 84, 188 |
| AclWI | GGATC | 4 cut(s) | 41, 948, 1147, 1330 |
| AcoI | YGGCCR | 1 cut(s) | 171 |
| AcsI | RAATTY | 2 cut(s) | 366, 1391 |
| AcuI | CTGAAG | 2 cut(s) | 381, 931 |
| AcyI | GRCGYC | 2 cut(s) | 122, 1629 |
| AfaI | GTAC | 3 cut(s) | 881, 1592, 1850 |
| AfiI | CCNNNNNNNGG | 7 cut(s) | 29, 302, 918, 1117, 1755, 1874, 1910 |
| AgsI | TTSAA | 9 cut(s) | 286, 463, 671, 746, 754, 1081, 1477, 1577, 1937 |
| AjnI | CCWGG | 4 cut(s) | 180, 295, 1324, 1743 |
| Alw21I | GWGCWC | 3 cut(s) | 796, 1007, 1095 |
| Alw26I | GTCTC | 6 cut(s) | 643, 684, 978, 1221, 1223, 1486 |
| AlwI | GGATC | 4 cut(s) | 41, 948, 1147, 1330 |
| AoxI | GGCC | 8 cut(s) | 171, 278, 408, 508, 825, 1119, 1746, 1782 |
| ApeKI | GCWGC | 7 cut(s) | 73, 356, 545, 1057, 1148, 1709, 1914 |
| ApoI | RAATTY | 2 cut(s) | 366, 1391 |
| AspS9I | GGNCC | 5 cut(s) | 20, 299, 825, 1865, 1925 |
| AsuC2I | CCSGG | 2 cut(s) | 303, 496 |
| AsuHPI | GGTGA | 7 cut(s) | 317, 602, 742, 1175, 1364, 1432, 1519 |
| AsuII | TTCGAA | 1 cut(s) | 924 |
| AvaII | GGWCC | 4 cut(s) | 20, 299, 1865, 1925 |
| BanI | GGYRCC | 1 cut(s) | 1133 |
| BanII | GRGCYC | 4 cut(s) | 540, 796, 1007, 1095 |
| BauI | CACGAG | 1 cut(s) | 55 |
| BbsI | GAAGAC | 1 cut(s) | 202 |
| Bbv12I | GWGCWC | 3 cut(s) | 796, 1007, 1095 |
| BbvCI | CCTCAGC | 1 cut(s) | 1779 |
| BbvI | GCAGC | 7 cut(s) | 85, 343, 532, 1069, 1135, 1721, 1926 |
| BccI | CCATC | 3 cut(s) | 988, 1532, 1564 |
| BceAI | ACGGC | 2 cut(s) | 109, 1106 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 62, 96 |
| BciT130I | CCWGG | 4 cut(s) | 182, 297, 1326, 1745 |
| BciVI | GTATCC | 1 cut(s) | 1726 |
| BcnI | CCSGG | 2 cut(s) | 303, 496 |
| BcoDI | GTCTC | 6 cut(s) | 643, 684, 978, 1221, 1223, 1486 |
| BfaI | CTAG | 3 cut(s) | 918, 1041, 1908 |
| BfmI | CTRYAG | 1 cut(s) | 1500 |
| BfuI | GTATCC | 1 cut(s) | 1726 |
| Bme1390I | CCNGG | 6 cut(s) | 182, 297, 303, 496, 1326, 1745 |
| Bme18I | GGWCC | 4 cut(s) | 20, 299, 1865, 1925 |
| BmgT120I | GGNCC | 5 cut(s) | 20, 299, 825, 1865, 1925 |
| BmiI | GGNNCC | 4 cut(s) | 301, 963, 1135, 1927 |
| BmrFI | CCNGG | 6 cut(s) | 182, 297, 303, 496, 1326, 1745 |
| BmsI | GCATC | 8 cut(s) | 157, 205, 381, 481, 510, 1450, 1696, 1830 |
| BpiI | GAAGAC | 1 cut(s) | 202 |
| BpmI | CTGGAG | 3 cut(s) | 708, 1257, 1664 |
| Bpu10I | CCTNAGC | 2 cut(s) | 1157, 1779 |
| Bpu14I | TTCGAA | 1 cut(s) | 924 |
| BpuEI | CTTGAG | 1 cut(s) | 1835 |
| BpuMI | CCSGG | 2 cut(s) | 303, 496 |
| Bsa29I | ATCGAT | 2 cut(s) | 36, 158 |
| BsaBI | GATNNNNATC | 2 cut(s) | 32, 1523 |
| BsaHI | GRCGYC | 2 cut(s) | 122, 1629 |
| BsaI | GGTCTC | 1 cut(s) | 1223 |
| BsaJI | CCNNGG | 8 cut(s) | 375, 486, 495, 1262, 1421, 1488, 1551, 1852 |
| Bsc4I | CCNNNNNNNGG | 7 cut(s) | 29, 302, 918, 1117, 1755, 1874, 1910 |
| Bse118I | RCCGGY | 1 cut(s) | 506 |
| Bse1I | ACTGG | 3 cut(s) | 582, 784, 965 |
| Bse3DI | GCAATG | 2 cut(s) | 501, 1836 |
| Bse8I | GATNNNNATC | 2 cut(s) | 32, 1523 |
| BseBI | CCWGG | 4 cut(s) | 182, 297, 1326, 1745 |
| BseCI | ATCGAT | 2 cut(s) | 36, 158 |
| BseDI | CCNNGG | 8 cut(s) | 375, 486, 495, 1262, 1421, 1488, 1551, 1852 |
| BseGI | GGATG | 4 cut(s) | 496, 842, 1687, 1894 |
| BseJI | GATNNNNATC | 2 cut(s) | 32, 1523 |
| BseLI | CCNNNNNNNGG | 7 cut(s) | 29, 302, 918, 1117, 1755, 1874, 1910 |
| BseMI | GCAATG | 2 cut(s) | 501, 1836 |
| BseMII | CTCAG | 5 cut(s) | 485, 866, 1148, 1575, 1770 |
| BseNI | ACTGG | 3 cut(s) | 582, 784, 965 |
| BseRI | GAGGAG | 4 cut(s) | 671, 720, 1200, 1622 |
| BseXI | GCAGC | 7 cut(s) | 85, 343, 532, 1069, 1135, 1721, 1926 |
| BsgI | GTGCAG | 2 cut(s) | 1076, 1163 |
| Bsh1236I | CGCG | 2 cut(s) | 84, 188 |
| Bsh1285I | CGRYCG | 3 cut(s) | 30, 82, 162 |
| BshFI | GGCC | 8 cut(s) | 173, 280, 410, 510, 827, 1121, 1748, 1784 |
| BshNI | GGYRCC | 1 cut(s) | 1133 |
| BshVI | ATCGAT | 2 cut(s) | 36, 158 |
| BsiEI | CGRYCG | 3 cut(s) | 30, 82, 162 |
| BsiHKAI | GWGCWC | 3 cut(s) | 796, 1007, 1095 |
| BsiSI | CCGG | 6 cut(s) | 23, 303, 452, 495, 507, 1404 |
| BslFI | GGGAC | 1 cut(s) | 1911 |
| BslI | CCNNNNNNNGG | 7 cut(s) | 29, 302, 918, 1117, 1755, 1874, 1910 |
| BsmAI | GTCTC | 6 cut(s) | 643, 684, 978, 1221, 1223, 1486 |
| BsmBI | CGTCTC | 1 cut(s) | 978 |
| BsmFI | GGGAC | 1 cut(s) | 1911 |
| BsmI | GAATGC | 3 cut(s) | 439, 1407, 1642 |
| BsnI | GGCC | 8 cut(s) | 173, 280, 410, 510, 827, 1121, 1748, 1784 |
| Bso31I | GGTCTC | 1 cut(s) | 1223 |
| Bsp119I | TTCGAA | 1 cut(s) | 924 |
| Bsp1286I | GDGCHC | 4 cut(s) | 540, 796, 1007, 1095 |
| Bsp19I | CCATGG | 1 cut(s) | 375 |
| Bsp68I | TCGCGA | 1 cut(s) | 188 |
| BspANI | GGCC | 8 cut(s) | 173, 280, 410, 510, 827, 1121, 1748, 1784 |
| BspCNI | CTCAG | 5 cut(s) | 484, 865, 1149, 1576, 1771 |
| BspDI | ATCGAT | 2 cut(s) | 36, 158 |
| BspFNI | CGCG | 2 cut(s) | 84, 188 |
| BspLI | GGNNCC | 4 cut(s) | 301, 963, 1135, 1927 |
| BspPI | GGATC | 4 cut(s) | 41, 948, 1147, 1330 |
| BspQI | GCTCTTC | 1 cut(s) | 921 |
| BspT104I | TTCGAA | 1 cut(s) | 924 |
| BspT107I | GGYRCC | 1 cut(s) | 1133 |
| BspTNI | GGTCTC | 1 cut(s) | 1223 |
| BsrBI | CCGCTC | 1 cut(s) | 1161 |
| BsrDI | GCAATG | 2 cut(s) | 501, 1836 |
| BsrFI | RCCGGY | 1 cut(s) | 506 |
| BsrI | ACTGG | 3 cut(s) | 582, 784, 965 |
| BssAI | RCCGGY | 1 cut(s) | 506 |
| BssECI | CCNNGG | 8 cut(s) | 375, 486, 495, 1262, 1421, 1488, 1551, 1852 |
| BssNAI | GTATAC | 1 cut(s) | 1345 |
| BssNI | GRCGYC | 2 cut(s) | 122, 1629 |
| BssSI | CACGAG | 1 cut(s) | 55 |
| BssT1I | CCWWGG | 5 cut(s) | 375, 486, 1421, 1551, 1852 |
| Bst1107I | GTATAC | 1 cut(s) | 1345 |
| Bst2BI | CACGAG | 1 cut(s) | 55 |
| Bst2UI | CCWGG | 4 cut(s) | 182, 297, 1326, 1745 |
| Bst4CI | ACNGT | 4 cut(s) | 208, 448, 1252, 1444 |
| Bst6I | CTCTTC | 3 cut(s) | 648, 921, 950 |
| BstACI | GRCGYC | 2 cut(s) | 122, 1629 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 1066 |
| BstBI | TTCGAA | 1 cut(s) | 924 |
| BstC8I | GCNNGC | 3 cut(s) | 508, 1146, 1776 |
| BstDEI | CTNAG | 6 cut(s) | 471, 797, 852, 1157, 1584, 1779 |
| BstDSI | CCRYGG | 1 cut(s) | 375 |
| BstF5I | GGATG | 4 cut(s) | 496, 842, 1687, 1894 |
| BstFNI | CGCG | 2 cut(s) | 84, 188 |
| BstMAI | GTCTC | 6 cut(s) | 643, 684, 978, 1221, 1223, 1486 |
| BstMCI | CGRYCG | 3 cut(s) | 30, 82, 162 |
| BstMWI | GCNNNNNNNGC | 4 cut(s) | 554, 1066, 1133, 1637 |
| BstNI | CCWGG | 4 cut(s) | 182, 297, 1326, 1745 |
| BstSCI | CCNGG | 6 cut(s) | 180, 295, 301, 494, 1324, 1743 |
| BstSFI | CTRYAG | 1 cut(s) | 1500 |
| BstUI | CGCG | 2 cut(s) | 84, 188 |
| BstV1I | GCAGC | 7 cut(s) | 85, 343, 532, 1069, 1135, 1721, 1926 |
| BstV2I | GAAGAC | 1 cut(s) | 202 |
| BstX2I | RGATCY | 1 cut(s) | 940 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 1859 |
| BstYI | RGATCY | 1 cut(s) | 940 |
| BstZ17I | GTATAC | 1 cut(s) | 1345 |
| Bsu15I | ATCGAT | 2 cut(s) | 36, 158 |
| BsuI | GTATCC | 1 cut(s) | 1726 |
| BsuRI | GGCC | 8 cut(s) | 173, 280, 410, 510, 827, 1121, 1748, 1784 |
| BsuTUI | ATCGAT | 2 cut(s) | 36, 158 |
| BtgI | CCRYGG | 1 cut(s) | 375 |
| BtgZI | GCGATG | 3 cut(s) | 79, 180, 203 |
| BtsCI | GGATG | 4 cut(s) | 496, 842, 1687, 1894 |
| BtsI | GCAGTG | 2 cut(s) | 540, 846 |
| BtsIMutI | CAGTG | 5 cut(s) | 540, 589, 777, 846, 1760 |
| BtuMI | TCGCGA | 1 cut(s) | 188 |
| Cac8I | GCNNGC | 3 cut(s) | 508, 1146, 1776 |
| Cfr10I | RCCGGY | 1 cut(s) | 506 |
| Cfr13I | GGNCC | 5 cut(s) | 20, 299, 825, 1865, 1925 |
| ClaI | ATCGAT | 2 cut(s) | 36, 158 |
| CseI | GACGC | 5 cut(s) | 49, 130, 464, 995, 1637 |
| CsiI | ACCWGGT | 1 cut(s) | 180 |
| Csp6I | GTAC | 3 cut(s) | 880, 1591, 1849 |
| CviAII | CATG | 7 cut(s) | 268, 316, 376, 937, 1013, 1663, 1885 |
| CviQI | GTAC | 3 cut(s) | 880, 1591, 1849 |
| DdeI | CTNAG | 6 cut(s) | 471, 797, 852, 1157, 1584, 1779 |
| DrdI | GACNNNNNNGTC | 2 cut(s) | 124, 254 |
| DseDI | GACNNNNNNGTC | 2 cut(s) | 124, 254 |
| EaeI | YGGCCR | 1 cut(s) | 171 |
| Eam1104I | CTCTTC | 3 cut(s) | 648, 921, 950 |
| EarI | CTCTTC | 3 cut(s) | 648, 921, 950 |
| Ecl136II | GAGCTC | 3 cut(s) | 794, 1005, 1093 |
| Eco130I | CCWWGG | 5 cut(s) | 375, 486, 1421, 1551, 1852 |
| Eco147I | AGGCCT | 1 cut(s) | 410 |
| Eco24I | GRGCYC | 4 cut(s) | 540, 796, 1007, 1095 |
| Eco31I | GGTCTC | 1 cut(s) | 1223 |
| Eco47I | GGWCC | 4 cut(s) | 20, 299, 1865, 1925 |
| Eco53kI | GAGCTC | 3 cut(s) | 794, 1005, 1093 |
| Eco57I | CTGAAG | 2 cut(s) | 381, 931 |
| EcoICRI | GAGCTC | 3 cut(s) | 794, 1005, 1093 |
| EcoO109I | RGGNCCY | 1 cut(s) | 299 |
| EcoRII | CCWGG | 4 cut(s) | 180, 295, 1324, 1743 |
| EcoT14I | CCWWGG | 5 cut(s) | 375, 486, 1421, 1551, 1852 |
| EcoT38I | GRGCYC | 4 cut(s) | 540, 796, 1007, 1095 |
| ErhI | CCWWGG | 5 cut(s) | 375, 486, 1421, 1551, 1852 |
| Esp3I | CGTCTC | 1 cut(s) | 978 |
| FaeI | CATG | 7 cut(s) | 271, 319, 379, 940, 1016, 1666, 1888 |
| FaqI | GGGAC | 1 cut(s) | 1911 |
| FatI | CATG | 7 cut(s) | 267, 315, 375, 936, 1012, 1662, 1884 |
| FauNDI | CATATG | 1 cut(s) | 559 |
| FblI | GTMKAC | 2 cut(s) | 891, 1344 |
| FokI | GGATG | 4 cut(s) | 503, 829, 1694, 1901 |
| FriOI | GRGCYC | 4 cut(s) | 540, 796, 1007, 1095 |
| FspBI | CTAG | 3 cut(s) | 918, 1041, 1908 |
| GsuI | CTGGAG | 3 cut(s) | 708, 1257, 1664 |
| HaeIII | GGCC | 8 cut(s) | 173, 280, 410, 510, 827, 1121, 1748, 1784 |
| HapII | CCGG | 6 cut(s) | 23, 303, 452, 495, 507, 1404 |
| HgaI | GACGC | 5 cut(s) | 49, 130, 464, 995, 1637 |
| Hin1I | GRCGYC | 2 cut(s) | 122, 1629 |
| Hin1II | CATG | 7 cut(s) | 271, 319, 379, 940, 1016, 1666, 1888 |
| HincII | GTYRAC | 2 cut(s) | 202, 892 |
| HindII | GTYRAC | 2 cut(s) | 202, 892 |
| HinfI | GANTC | 6 cut(s) | 240, 467, 835, 872, 1045, 1258 |
| HpaII | CCGG | 6 cut(s) | 23, 303, 452, 495, 507, 1404 |
| HphI | GGTGA | 7 cut(s) | 317, 602, 742, 1175, 1364, 1432, 1519 |
| Hpy166II | GTNNAC | 5 cut(s) | 202, 733, 892, 1345, 1627 |
| Hpy188I | TCNGA | 7 cut(s) | 44, 247, 855, 1050, 1263, 1315, 1600 |
| Hpy8I | GTNNAC | 5 cut(s) | 202, 733, 892, 1345, 1627 |
| Hpy99I | CGWCG | 3 cut(s) | 65, 124, 1790 |
| HpyCH4III | ACNGT | 4 cut(s) | 208, 448, 1252, 1444 |
| HpyCH4IV | ACGT | 1 cut(s) | 516 |
| HpyCH4V | TGCA | 7 cut(s) | 557, 1057, 1069, 1098, 1144, 1709, 1827 |
| HpyF10VI | GCNNNNNNNGC | 4 cut(s) | 554, 1066, 1133, 1637 |
| HpyF3I | CTNAG | 6 cut(s) | 471, 797, 852, 1157, 1584, 1779 |
| HpySE526I | ACGT | 1 cut(s) | 516 |
| Hsp92I | GRCGYC | 2 cut(s) | 122, 1629 |
| Hsp92II | CATG | 7 cut(s) | 271, 319, 379, 940, 1016, 1666, 1888 |
| KroI | GCCGGC | 1 cut(s) | 506 |
| KroNI | GCCGGC | 1 cut(s) | 508 |
| LguI | GCTCTTC | 1 cut(s) | 921 |
| LmnI | GCTCC | 6 cut(s) | 961, 1090, 1291, 1423, 1609, 1810 |
| Lsp1109I | GCAGC | 7 cut(s) | 85, 343, 532, 1069, 1135, 1721, 1926 |
| LweI | GCATC | 8 cut(s) | 157, 205, 381, 481, 510, 1450, 1696, 1830 |
| MabI | ACCWGGT | 1 cut(s) | 180 |
| MaeI | CTAG | 3 cut(s) | 918, 1041, 1908 |
| MaeII | ACGT | 1 cut(s) | 516 |
| MaeIII | GTNAC | 6 cut(s) | 263, 305, 325, 442, 1169, 1204 |
| MbiI | CCGCTC | 1 cut(s) | 1161 |
| MflI | RGATCY | 1 cut(s) | 940 |
| MhlI | GDGCHC | 4 cut(s) | 540, 796, 1007, 1095 |
| MluCI | AATT | 2 cut(s) | 366, 1391 |
| MlyI | GAGTC | 2 cut(s) | 461, 829 |
| MmeI | TCCRAC | 2 cut(s) | 1304, 1578 |
| MroNI | GCCGGC | 1 cut(s) | 506 |
| MseI | TTAA | 6 cut(s) | 387, 513, 933, 980, 1677, 1948 |
| MslI | CAYNNNNRTG | 1 cut(s) | 389 |
| MspA1I | CMGCKG | 3 cut(s) | 1235, 1712, 1917 |
| MspI | CCGG | 6 cut(s) | 23, 303, 452, 495, 507, 1404 |
| MspR9I | CCNGG | 6 cut(s) | 182, 297, 303, 496, 1326, 1745 |
| Mva1269I | GAATGC | 3 cut(s) | 439, 1407, 1642 |
| MvaI | CCWGG | 4 cut(s) | 182, 297, 1326, 1745 |
| MvnI | CGCG | 2 cut(s) | 84, 188 |
| MwoI | GCNNNNNNNGC | 4 cut(s) | 554, 1066, 1133, 1637 |
| NaeI | GCCGGC | 1 cut(s) | 508 |
| NciI | CCSGG | 2 cut(s) | 303, 496 |
| NcoI | CCATGG | 1 cut(s) | 375 |
| NdeI | CATATG | 1 cut(s) | 559 |
| NgoMIV | GCCGGC | 1 cut(s) | 506 |
| NlaIII | CATG | 7 cut(s) | 271, 319, 379, 940, 1016, 1666, 1888 |
| NlaIV | GGNNCC | 4 cut(s) | 301, 963, 1135, 1927 |
| NmeAIII | GCCGAG | 1 cut(s) | 1513 |
| NmuCI | GTSAC | 4 cut(s) | 263, 305, 325, 1204 |
| NruI | TCGCGA | 1 cut(s) | 188 |
| NspV | TTCGAA | 1 cut(s) | 924 |
| PceI | AGGCCT | 1 cut(s) | 410 |
| PciSI | GCTCTTC | 1 cut(s) | 921 |
| PcsI | WCGNNNNNNNCGW | 2 cut(s) | 53, 888 |
| PctI | GAATGC | 3 cut(s) | 439, 1407, 1642 |
| PdiI | GCCGGC | 1 cut(s) | 508 |
| PfeI | GAWTC | 4 cut(s) | 240, 872, 1045, 1258 |
| PflFI | GACNNNGTC | 1 cut(s) | 124 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 1755 |
| PfoI | TCCNGGA | 1 cut(s) | 295 |
| Ple19I | CGATCG | 3 cut(s) | 30, 82, 162 |
| PleI | GAGTC | 2 cut(s) | 461, 829 |
| PpsI | GAGTC | 2 cut(s) | 461, 829 |
| PpuMI | RGGWCCY | 1 cut(s) | 299 |
| Psp124BI | GAGCTC | 3 cut(s) | 796, 1007, 1095 |
| Psp5II | RGGWCCY | 1 cut(s) | 299 |
| Psp6I | CCWGG | 4 cut(s) | 180, 295, 1324, 1743 |
| PspGI | CCWGG | 4 cut(s) | 180, 295, 1324, 1743 |
| PspN4I | GGNNCC | 4 cut(s) | 301, 963, 1135, 1927 |
| PspPI | GGNCC | 5 cut(s) | 20, 299, 825, 1865, 1925 |
| PspPPI | RGGWCCY | 1 cut(s) | 299 |
| PsrI | GAACNNNNNNTAC | 2 cut(s) | 1640, 1672 |
| PsuI | RGATCY | 1 cut(s) | 940 |
| PsyI | GACNNNGTC | 1 cut(s) | 124 |
| PvuI | CGATCG | 3 cut(s) | 30, 82, 162 |
| PvuII | CAGCTG | 1 cut(s) | 1712 |
| RruI | TCGCGA | 1 cut(s) | 188 |
| RsaI | GTAC | 3 cut(s) | 881, 1592, 1850 |
| RsaNI | GTAC | 3 cut(s) | 880, 1591, 1849 |
| RseI | CAYNNNNRTG | 1 cut(s) | 389 |
| SacI | GAGCTC | 3 cut(s) | 796, 1007, 1095 |
| SalI | GTCGAC | 1 cut(s) | 890 |
| SapI | GCTCTTC | 1 cut(s) | 921 |
| SaqAI | TTAA | 6 cut(s) | 387, 513, 933, 980, 1677, 1948 |
| Sau96I | GGNCC | 5 cut(s) | 20, 299, 825, 1865, 1925 |
| SchI | GAGTC | 2 cut(s) | 461, 829 |
| ScrFI | CCNGG | 6 cut(s) | 182, 297, 303, 496, 1326, 1745 |
| SduI | GDGCHC | 4 cut(s) | 540, 796, 1007, 1095 |
| SexAI | ACCWGGT | 1 cut(s) | 180 |
| SfaNI | GCATC | 8 cut(s) | 157, 205, 381, 481, 510, 1450, 1696, 1830 |
| SfcI | CTRYAG | 1 cut(s) | 1500 |
| SfuI | TTCGAA | 1 cut(s) | 924 |
| SinI | GGWCC | 4 cut(s) | 20, 299, 1865, 1925 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 389 |
| SmlI | CTYRAG | 1 cut(s) | 1814 |
| SmoI | CTYRAG | 1 cut(s) | 1814 |
| Sse9I | AATT | 2 cut(s) | 366, 1391 |
| SseBI | AGGCCT | 1 cut(s) | 410 |
| SspMI | CTAG | 3 cut(s) | 918, 1041, 1908 |
| SstI | GAGCTC | 3 cut(s) | 796, 1007, 1095 |
| StuI | AGGCCT | 1 cut(s) | 410 |
| StyD4I | CCNGG | 6 cut(s) | 180, 295, 301, 494, 1324, 1743 |
| StyI | CCWWGG | 5 cut(s) | 375, 486, 1421, 1551, 1852 |
| TaaI | ACNGT | 4 cut(s) | 208, 448, 1252, 1444 |
| TaiI | ACGT | 1 cut(s) | 519 |
| TasI | AATT | 2 cut(s) | 366, 1391 |
| TatI | WGTACW | 1 cut(s) | 1590 |
| TauI | GCSGC | 3 cut(s) | 173, 551, 1884 |
| TfiI | GAWTC | 4 cut(s) | 240, 872, 1045, 1258 |
| Tru1I | TTAA | 6 cut(s) | 387, 513, 933, 980, 1677, 1948 |
| Tru9I | TTAA | 6 cut(s) | 387, 513, 933, 980, 1677, 1948 |
| TscAI | CASTG | 5 cut(s) | 547, 589, 784, 853, 1760 |
| TseFI | GTSAC | 4 cut(s) | 263, 305, 325, 1204 |
| TseI | GCWGC | 7 cut(s) | 73, 356, 545, 1057, 1148, 1709, 1914 |
| Tsp45I | GTSAC | 4 cut(s) | 263, 305, 325, 1204 |
| TspDTI | ATGAA | 4 cut(s) | 206, 284, 411, 1820 |
| TspGWI | ACGGA | 1 cut(s) | 1779 |
| TspRI | CASTG | 5 cut(s) | 547, 589, 784, 853, 1760 |
| Tth111I | GACNNNGTC | 1 cut(s) | 124 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 1755 |
| VpaK11BI | GGWCC | 4 cut(s) | 20, 299, 1865, 1925 |
| XapI | RAATTY | 2 cut(s) | 366, 1391 |
| XcmI | CCANNNNNNNNNTGG | 2 cut(s) | 1639, 1859 |
| XmiI | GTMKAC | 2 cut(s) | 891, 1344 |
| XspI | CTAG | 3 cut(s) | 918, 1041, 1908 |