Belongs to the heat shock protein 70 family
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AatII | GACGTC | 1 cut(s) | 456 |
| AccB1I | GGYRCC | 1 cut(s) | 812 |
| AccI | GTMKAC | 1 cut(s) | 530 |
| AciI | CCGC | 2 cut(s) | 816, 1131 |
| AclWI | GGATC | 1 cut(s) | 160 |
| AcoI | YGGCCR | 2 cut(s) | 11, 1210 |
| AcsI | RAATTY | 2 cut(s) | 599, 981 |
| AcuI | CTGAAG | 2 cut(s) | 114, 159 |
| AcyI | GRCGYC | 1 cut(s) | 453 |
| AdeI | CACNNNGTG | 1 cut(s) | 812 |
| AfaI | GTAC | 2 cut(s) | 324, 852 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 211, 796 |
| AflII | CTTAAG | 1 cut(s) | 1100 |
| AflIII | ACRYGT | 1 cut(s) | 1200 |
| AgsI | TTSAA | 8 cut(s) | 19, 196, 350, 433, 545, 605, 838, 866 |
| AjnI | CCWGG | 1 cut(s) | 853 |
| AjuI | GAANNNNNNNTTGG | 4 cut(s) | 132, 164, 431, 463 |
| AluBI | AGCT | 8 cut(s) | 7, 143, 239, 772, 1041, 1105, 1124, 1193 |
| AluI | AGCT | 8 cut(s) | 7, 143, 239, 772, 1041, 1105, 1124, 1193 |
| AlwI | GGATC | 1 cut(s) | 160 |
| Ama87I | CYCGRG | 1 cut(s) | 976 |
| AoxI | GGCC | 4 cut(s) | 11, 241, 295, 1210 |
| ApeKI | GCWGC | 4 cut(s) | 89, 278, 281, 827 |
| ApoI | RAATTY | 2 cut(s) | 599, 981 |
| Asp700I | GAANNNNTTC | 2 cut(s) | 517, 755 |
| AspS9I | GGNCC | 1 cut(s) | 32 |
| AsuHPI | GGTGA | 4 cut(s) | 262, 398, 880, 1070 |
| AvaI | CYCGRG | 1 cut(s) | 976 |
| AvaII | GGWCC | 1 cut(s) | 32 |
| AxyI | CCTNAGG | 1 cut(s) | 989 |
| BalI | TGGCCA | 2 cut(s) | 13, 1212 |
| BanI | GGYRCC | 1 cut(s) | 812 |
| BbsI | GAAGAC | 3 cut(s) | 396, 520, 708 |
| BbvCI | CCTCAGC | 1 cut(s) | 1194 |
| BbvI | GCAGC | 4 cut(s) | 76, 265, 293, 814 |
| BccI | CCATC | 3 cut(s) | 332, 926, 995 |
| BceAI | ACGGC | 1 cut(s) | 931 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 257, 291 |
| BciT130I | CCWGG | 1 cut(s) | 855 |
| BciVI | GTATCC | 1 cut(s) | 1187 |
| BfaI | CTAG | 1 cut(s) | 36 |
| BfmI | CTRYAG | 3 cut(s) | 279, 801, 828 |
| BfrI | CTTAAG | 1 cut(s) | 1100 |
| BfuI | GTATCC | 1 cut(s) | 1187 |
| BisI | GCNGC | 5 cut(s) | 90, 279, 282, 816, 828 |
| BlsI | GCNGC | 5 cut(s) | 91, 280, 283, 817, 829 |
| BmcAI | AGTACT | 1 cut(s) | 324 |
| Bme1390I | CCNGG | 1 cut(s) | 855 |
| Bme18I | GGWCC | 1 cut(s) | 32 |
| BmeT110I | CYCGRG | 1 cut(s) | 976 |
| BmgT120I | GGNCC | 1 cut(s) | 32 |
| BmiI | GGNNCC | 3 cut(s) | 642, 814, 945 |
| BmrFI | CCNGG | 1 cut(s) | 855 |
| BmsI | GCATC | 5 cut(s) | 114, 243, 568, 655, 1156 |
| BpiI | GAAGAC | 3 cut(s) | 396, 520, 708 |
| BpmI | CTGGAG | 2 cut(s) | 387, 1104 |
| Bpu10I | CCTNAGC | 1 cut(s) | 1194 |
| BpuEI | CTTGAG | 1 cut(s) | 982 |
| BsaAI | YACGTR | 1 cut(s) | 528 |
| BsaBI | GATNNNNATC | 1 cut(s) | 1229 |
| BsaHI | GRCGYC | 1 cut(s) | 453 |
| BsaJI | CCNNGG | 4 cut(s) | 148, 379, 729, 854 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 211, 796 |
| Bse118I | RCCGGY | 1 cut(s) | 707 |
| Bse1I | ACTGG | 3 cut(s) | 1213, 1251, 1268 |
| Bse21I | CCTNAGG | 1 cut(s) | 989 |
| Bse3DI | GCAATG | 2 cut(s) | 285, 1113 |
| Bse8I | GATNNNNATC | 1 cut(s) | 1229 |
| BseBI | CCWGG | 1 cut(s) | 855 |
| BseDI | CCNNGG | 4 cut(s) | 148, 379, 729, 854 |
| BseGI | GGATG | 4 cut(s) | 343, 670, 697, 889 |
| BseJI | GATNNNNATC | 1 cut(s) | 1229 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 211, 796 |
| BseMI | GCAATG | 2 cut(s) | 285, 1113 |
| BseMII | CTCAG | 3 cut(s) | 980, 1172, 1185 |
| BseNI | ACTGG | 3 cut(s) | 1213, 1251, 1268 |
| BseXI | GCAGC | 4 cut(s) | 76, 265, 293, 814 |
| BseYI | CCCAGC | 1 cut(s) | 1127 |
| BshFI | GGCC | 4 cut(s) | 13, 243, 297, 1212 |
| BshNI | GGYRCC | 1 cut(s) | 812 |
| BsiHKCI | CYCGRG | 1 cut(s) | 976 |
| BsiSI | CCGG | 1 cut(s) | 708 |
| BslFI | GGGAC | 1 cut(s) | 167 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 211, 796 |
| BsmFI | GGGAC | 1 cut(s) | 167 |
| BsnI | GGCC | 4 cut(s) | 13, 243, 297, 1212 |
| BsoBI | CYCGRG | 1 cut(s) | 976 |
| Bsp143I | GATC | 2 cut(s) | 152, 1230 |
| BspACI | CCGC | 2 cut(s) | 816, 1131 |
| BspANI | GGCC | 4 cut(s) | 13, 243, 297, 1212 |
| BspCNI | CTCAG | 3 cut(s) | 981, 1173, 1186 |
| BspHI | TCATGA | 1 cut(s) | 951 |
| BspLI | GGNNCC | 3 cut(s) | 642, 814, 945 |
| BspMAI | CTGCAG | 2 cut(s) | 283, 832 |
| BspPI | GGATC | 1 cut(s) | 160 |
| BspT107I | GGYRCC | 1 cut(s) | 812 |
| BspTI | CTTAAG | 1 cut(s) | 1100 |
| BsrDI | GCAATG | 2 cut(s) | 285, 1113 |
| BsrFI | RCCGGY | 1 cut(s) | 707 |
| BsrI | ACTGG | 3 cut(s) | 1213, 1251, 1268 |
| BssAI | RCCGGY | 1 cut(s) | 707 |
| BssECI | CCNNGG | 4 cut(s) | 148, 379, 729, 854 |
| BssMI | GATC | 2 cut(s) | 152, 1230 |
| BssNAI | GTATAC | 1 cut(s) | 531 |
| BssNI | GRCGYC | 1 cut(s) | 453 |
| BssT1I | CCWWGG | 3 cut(s) | 148, 379, 729 |
| Bst1107I | GTATAC | 1 cut(s) | 531 |
| Bst2UI | CCWGG | 1 cut(s) | 855 |
| Bst4CI | ACNGT | 4 cut(s) | 160, 181, 1015, 1238 |
| Bst6I | CTCTTC | 1 cut(s) | 989 |
| BstACI | GRCGYC | 1 cut(s) | 453 |
| BstAFI | CTTAAG | 1 cut(s) | 1100 |
| BstAPI | GCANNNNNTGC | 2 cut(s) | 287, 812 |
| BstBAI | YACGTR | 1 cut(s) | 528 |
| BstC8I | GCNNGC | 2 cut(s) | 229, 241 |
| BstDEI | CTNAG | 4 cut(s) | 476, 989, 1181, 1194 |
| BstEII | GGTNACC | 1 cut(s) | 58 |
| BstF5I | GGATG | 4 cut(s) | 343, 670, 697, 889 |
| BstKTI | GATC | 2 cut(s) | 155, 1233 |
| BstMBI | GATC | 2 cut(s) | 152, 1230 |
| BstMWI | GCNNNNNNNGC | 3 cut(s) | 287, 812, 824 |
| BstNI | CCWGG | 1 cut(s) | 855 |
| BstNSI | RCATGY | 2 cut(s) | 227, 1204 |
| BstPI | GGTNACC | 1 cut(s) | 58 |
| BstSCI | CCNGG | 1 cut(s) | 853 |
| BstSFI | CTRYAG | 3 cut(s) | 279, 801, 828 |
| BstSNI | TACGTA | 1 cut(s) | 528 |
| BstV1I | GCAGC | 4 cut(s) | 76, 265, 293, 814 |
| BstV2I | GAAGAC | 3 cut(s) | 396, 520, 708 |
| BstZ17I | GTATAC | 1 cut(s) | 531 |
| Bsu36I | CCTNAGG | 1 cut(s) | 989 |
| BsuI | GTATCC | 1 cut(s) | 1187 |
| BsuRI | GGCC | 4 cut(s) | 13, 243, 297, 1212 |
| BtsCI | GGATG | 4 cut(s) | 343, 670, 697, 889 |
| BtsI | GCAGTG | 2 cut(s) | 360, 691 |
| BtsIMutI | CAGTG | 8 cut(s) | 177, 360, 463, 691, 1206, 1243, 1258, 1275 |
| Cac8I | GCNNGC | 2 cut(s) | 229, 241 |
| CciI | TCATGA | 1 cut(s) | 951 |
| Cfr10I | RCCGGY | 1 cut(s) | 707 |
| Cfr13I | GGNCC | 1 cut(s) | 32 |
| CseI | GACGC | 1 cut(s) | 409 |
| Csp6I | GTAC | 2 cut(s) | 323, 851 |
| CviAII | CATG | 6 cut(s) | 224, 443, 760, 809, 952, 1201 |
| CviQI | GTAC | 2 cut(s) | 323, 851 |
| DdeI | CTNAG | 4 cut(s) | 476, 989, 1181, 1194 |
| DpnI | GATC | 2 cut(s) | 154, 1232 |
| DpnII | GATC | 2 cut(s) | 152, 1230 |
| DraIII | CACNNNGTG | 1 cut(s) | 812 |
| EaeI | YGGCCR | 2 cut(s) | 11, 1210 |
| Eam1104I | CTCTTC | 1 cut(s) | 989 |
| EarI | CTCTTC | 1 cut(s) | 989 |
| Eco105I | TACGTA | 1 cut(s) | 528 |
| Eco130I | CCWWGG | 3 cut(s) | 148, 379, 729 |
| Eco47I | GGWCC | 1 cut(s) | 32 |
| Eco57I | CTGAAG | 2 cut(s) | 114, 159 |
| Eco81I | CCTNAGG | 1 cut(s) | 989 |
| Eco88I | CYCGRG | 1 cut(s) | 976 |
| Eco91I | GGTNACC | 1 cut(s) | 58 |
| EcoO109I | RGGNCCY | 1 cut(s) | 32 |
| EcoO65I | GGTNACC | 1 cut(s) | 58 |
| EcoRI | GAATTC | 1 cut(s) | 981 |
| EcoRII | CCWGG | 1 cut(s) | 853 |
| EcoT14I | CCWWGG | 3 cut(s) | 148, 379, 729 |
| ErhI | CCWWGG | 3 cut(s) | 148, 379, 729 |
| FaeI | CATG | 6 cut(s) | 227, 446, 763, 812, 955, 1204 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 132, 164 |
| FaqI | GGGAC | 1 cut(s) | 167 |
| FatI | CATG | 6 cut(s) | 223, 442, 759, 808, 951, 1200 |
| FblI | GTMKAC | 1 cut(s) | 530 |
| Fnu4HI | GCNGC | 5 cut(s) | 90, 279, 282, 816, 828 |
| FokI | GGATG | 4 cut(s) | 350, 677, 704, 896 |
| Fsp4HI | GCNGC | 5 cut(s) | 90, 279, 282, 816, 828 |
| FspBI | CTAG | 1 cut(s) | 36 |
| GluI | GCNGC | 5 cut(s) | 90, 279, 282, 816, 828 |
| GsaI | CCCAGC | 1 cut(s) | 1131 |
| GsuI | CTGGAG | 2 cut(s) | 387, 1104 |
| HaeIII | GGCC | 4 cut(s) | 13, 243, 297, 1212 |
| HapII | CCGG | 1 cut(s) | 708 |
| HgaI | GACGC | 1 cut(s) | 409 |
| Hin1I | GRCGYC | 1 cut(s) | 453 |
| Hin1II | CATG | 6 cut(s) | 227, 446, 763, 812, 955, 1204 |
| HincII | GTYRAC | 1 cut(s) | 412 |
| HindII | GTYRAC | 1 cut(s) | 412 |
| HindIII | AAGCTT | 2 cut(s) | 141, 1103 |
| HinfI | GANTC | 3 cut(s) | 723, 964, 1143 |
| HpaI | GTTAAC | 1 cut(s) | 412 |
| HpaII | CCGG | 1 cut(s) | 708 |
| HphI | GGTGA | 4 cut(s) | 262, 398, 880, 1070 |
| Hpy166II | GTNNAC | 4 cut(s) | 412, 525, 531, 1206 |
| Hpy188I | TCNGA | 1 cut(s) | 51 |
| Hpy188III | TCNNGA | 7 cut(s) | 433, 720, 866, 952, 961, 978, 1180 |
| Hpy8I | GTNNAC | 4 cut(s) | 412, 525, 531, 1206 |
| Hpy99I | CGWCG | 1 cut(s) | 425 |
| HpyAV | CCTTC | 2 cut(s) | 760, 1250 |
| HpyCH4III | ACNGT | 4 cut(s) | 160, 181, 1015, 1238 |
| HpyCH4IV | ACGT | 3 cut(s) | 208, 453, 527 |
| HpyCH4V | TGCA | 6 cut(s) | 119, 281, 559, 736, 777, 830 |
| HpyF10VI | GCNNNNNNNGC | 3 cut(s) | 287, 812, 824 |
| HpyF3I | CTNAG | 4 cut(s) | 476, 989, 1181, 1194 |
| HpySE526I | ACGT | 3 cut(s) | 208, 453, 527 |
| Hsp92I | GRCGYC | 1 cut(s) | 453 |
| Hsp92II | CATG | 6 cut(s) | 227, 446, 763, 812, 955, 1204 |
| KspAI | GTTAAC | 1 cut(s) | 412 |
| Kzo9I | GATC | 2 cut(s) | 152, 1230 |
| LmnI | GCTCC | 5 cut(s) | 640, 769, 943, 1123, 1129 |
| Lsp1109I | GCAGC | 4 cut(s) | 76, 265, 293, 814 |
| LweI | GCATC | 5 cut(s) | 114, 243, 568, 655, 1156 |
| MaeI | CTAG | 1 cut(s) | 36 |
| MaeII | ACGT | 3 cut(s) | 208, 453, 527 |
| MaeIII | GTNAC | 6 cut(s) | 58, 580, 886, 897, 1006, 1238 |
| MalI | GATC | 2 cut(s) | 154, 1232 |
| MboI | GATC | 2 cut(s) | 152, 1230 |
| MboII | GAAGA | 7 cut(s) | 107, 401, 525, 616, 708, 1006, 1257 |
| MlsI | TGGCCA | 2 cut(s) | 13, 1212 |
| MluCI | AATT | 8 cut(s) | 24, 132, 428, 546, 599, 648, 981, 1260 |
| MluNI | TGGCCA | 2 cut(s) | 13, 1212 |
| MlyI | GAGTC | 1 cut(s) | 973 |
| Mox20I | TGGCCA | 2 cut(s) | 13, 1212 |
| MroXI | GAANNNNTTC | 2 cut(s) | 517, 755 |
| MscI | TGGCCA | 2 cut(s) | 13, 1212 |
| MseI | TTAA | 6 cut(s) | 264, 411, 485, 630, 651, 1101 |
| Msp20I | TGGCCA | 2 cut(s) | 13, 1212 |
| MspA1I | CMGCKG | 3 cut(s) | 818, 1131, 1193 |
| MspCI | CTTAAG | 1 cut(s) | 1100 |
| MspI | CCGG | 1 cut(s) | 708 |
| MspR9I | CCNGG | 1 cut(s) | 855 |
| MvaI | CCWGG | 1 cut(s) | 855 |
| MwoI | GCNNNNNNNGC | 3 cut(s) | 287, 812, 824 |
| NdeII | GATC | 2 cut(s) | 152, 1230 |
| NlaIII | CATG | 6 cut(s) | 227, 446, 763, 812, 955, 1204 |
| NlaIV | GGNNCC | 3 cut(s) | 642, 814, 945 |
| NmuCI | GTSAC | 3 cut(s) | 886, 897, 1238 |
| NspI | RCATGY | 2 cut(s) | 227, 1204 |
| PaeR7I | CTCGAG | 1 cut(s) | 976 |
| PagI | TCATGA | 1 cut(s) | 951 |
| PciI | ACATGT | 1 cut(s) | 1200 |
| PdmI | GAANNNNTTC | 2 cut(s) | 517, 755 |
| PfeI | GAWTC | 2 cut(s) | 723, 1143 |
| PkrI | GCNGC | 5 cut(s) | 91, 280, 283, 817, 829 |
| PleI | GAGTC | 1 cut(s) | 972 |
| PpsI | GAGTC | 1 cut(s) | 972 |
| Ppu21I | YACGTR | 1 cut(s) | 528 |
| PpuMI | RGGWCCY | 1 cut(s) | 32 |
| PscI | ACATGT | 1 cut(s) | 1200 |
| Psp5II | RGGWCCY | 1 cut(s) | 32 |
| Psp6I | CCWGG | 1 cut(s) | 853 |
| PspEI | GGTNACC | 1 cut(s) | 58 |
| PspFI | CCCAGC | 1 cut(s) | 1127 |
| PspGI | CCWGG | 1 cut(s) | 853 |
| PspN4I | GGNNCC | 3 cut(s) | 642, 814, 945 |
| PspPI | GGNCC | 1 cut(s) | 32 |
| PspPPI | RGGWCCY | 1 cut(s) | 32 |
| PstI | CTGCAG | 2 cut(s) | 283, 832 |
| PvuII | CAGCTG | 1 cut(s) | 1193 |
| RsaI | GTAC | 2 cut(s) | 324, 852 |
| RsaNI | GTAC | 2 cut(s) | 323, 851 |
| SaqAI | TTAA | 6 cut(s) | 264, 411, 485, 630, 651, 1101 |
| SatI | GCNGC | 5 cut(s) | 90, 279, 282, 816, 828 |
| Sau3AI | GATC | 2 cut(s) | 152, 1230 |
| Sau96I | GGNCC | 1 cut(s) | 32 |
| ScaI | AGTACT | 1 cut(s) | 324 |
| SchI | GAGTC | 1 cut(s) | 973 |
| ScrFI | CCNGG | 1 cut(s) | 855 |
| SfaNI | GCATC | 5 cut(s) | 114, 243, 568, 655, 1156 |
| SfcI | CTRYAG | 3 cut(s) | 279, 801, 828 |
| Sfr274I | CTCGAG | 1 cut(s) | 976 |
| SinI | GGWCC | 1 cut(s) | 32 |
| SlaI | CTCGAG | 1 cut(s) | 976 |
| SmlI | CTYRAG | 3 cut(s) | 961, 976, 1100 |
| SmoI | CTYRAG | 3 cut(s) | 961, 976, 1100 |
| SnaBI | TACGTA | 1 cut(s) | 528 |
| Sse9I | AATT | 8 cut(s) | 24, 132, 428, 546, 599, 648, 981, 1260 |
| SsiI | CCGC | 2 cut(s) | 816, 1131 |
| SspI | AATATT | 1 cut(s) | 83 |
| SspMI | CTAG | 1 cut(s) | 36 |
| StyD4I | CCNGG | 1 cut(s) | 853 |
| StyI | CCWWGG | 3 cut(s) | 148, 379, 729 |
| TaaI | ACNGT | 4 cut(s) | 160, 181, 1015, 1238 |
| TaiI | ACGT | 3 cut(s) | 211, 456, 530 |
| TaqI | TCGA | 4 cut(s) | 396, 423, 721, 977 |
| TasI | AATT | 8 cut(s) | 24, 132, 428, 546, 599, 648, 981, 1260 |
| TatI | WGTACW | 1 cut(s) | 322 |
| TauI | GCSGC | 1 cut(s) | 818 |
| TfiI | GAWTC | 2 cut(s) | 723, 1143 |
| Tru1I | TTAA | 6 cut(s) | 264, 411, 485, 630, 651, 1101 |
| Tru9I | TTAA | 6 cut(s) | 264, 411, 485, 630, 651, 1101 |
| TscAI | CASTG | 8 cut(s) | 184, 367, 463, 691, 1213, 1243, 1258, 1275 |
| TseFI | GTSAC | 3 cut(s) | 886, 897, 1238 |
| TseI | GCWGC | 4 cut(s) | 89, 278, 281, 827 |
| Tsp45I | GTSAC | 3 cut(s) | 886, 897, 1238 |
| TspDTI | ATGAA | 4 cut(s) | 17, 748, 940, 968 |
| TspRI | CASTG | 8 cut(s) | 184, 367, 463, 691, 1213, 1243, 1258, 1275 |
| Vha464I | CTTAAG | 1 cut(s) | 1100 |
| VpaK11BI | GGWCC | 1 cut(s) | 32 |
| XapI | RAATTY | 2 cut(s) | 599, 981 |
| XceI | RCATGY | 2 cut(s) | 227, 1204 |
| XhoI | CTCGAG | 1 cut(s) | 976 |
| XmiI | GTMKAC | 1 cut(s) | 530 |
| XmnI | GAANNNNTTC | 2 cut(s) | 517, 755 |
| XspI | CTAG | 1 cut(s) | 36 |
| ZraI | GACGTC | 1 cut(s) | 454 |
| ZrmI | AGTACT | 1 cut(s) | 324 |