Heat shock cognate 70 kDa
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AasI | GACNNNNNNGTC | 1 cut(s) | 121 |
| AclWI | GGATC | 1 cut(s) | 1024 |
| AcoI | YGGCCR | 2 cut(s) | 183, 275 |
| AcsI | RAATTY | 5 cut(s) | 363, 662, 833, 1047, 1163 |
| AcuI | CTGAAG | 1 cut(s) | 378 |
| AfaI | GTAC | 5 cut(s) | 50, 538, 568, 1085, 1297 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 1431 |
| AflIII | ACRYGT | 1 cut(s) | 248 |
| AjnI | CCWGG | 3 cut(s) | 36, 177, 701 |
| AleI | CACNNNNGTG | 2 cut(s) | 78, 615 |
| AluBI | AGCT | 9 cut(s) | 153, 170, 271, 488, 917, 923, 1190, 1202, 1369 |
| AluI | AGCT | 9 cut(s) | 153, 170, 271, 488, 917, 923, 1190, 1202, 1369 |
| Alw21I | GWGCWC | 1 cut(s) | 1204 |
| Alw26I | GTCTC | 5 cut(s) | 80, 992, 999, 1261, 1421 |
| AlwI | GGATC | 1 cut(s) | 1024 |
| Ama87I | CYCGRG | 1 cut(s) | 1002 |
| AoxI | GGCC | 3 cut(s) | 183, 275, 334 |
| ApeKI | GCWGC | 7 cut(s) | 70, 353, 404, 407, 905, 917, 920 |
| ApoI | RAATTY | 5 cut(s) | 363, 662, 833, 1047, 1163 |
| AspS9I | GGNCC | 1 cut(s) | 874 |
| AsuHPI | GGTGA | 7 cut(s) | 130, 187, 602, 632, 655, 967, 1292 |
| AsuII | TTCGAA | 1 cut(s) | 837 |
| AvaI | CYCGRG | 1 cut(s) | 1002 |
| AvaII | GGWCC | 1 cut(s) | 874 |
| BalI | TGGCCA | 2 cut(s) | 185, 277 |
| BanII | GRGCYC | 1 cut(s) | 1204 |
| BauI | CACGAG | 1 cut(s) | 52 |
| BbsI | GAAGAC | 1 cut(s) | 630 |
| Bbv12I | GWGCWC | 1 cut(s) | 1204 |
| BbvI | GCAGC | 7 cut(s) | 82, 340, 391, 394, 892, 904, 932 |
| BccI | CCATC | 3 cut(s) | 158, 1178, 1232 |
| BceAI | ACGGC | 1 cut(s) | 810 |
| BcgI | CGANNNNNNTGC | 4 cut(s) | 59, 93, 434, 468 |
| BciT130I | CCWGG | 3 cut(s) | 38, 179, 703 |
| BciVI | GTATCC | 1 cut(s) | 1253 |
| BcoDI | GTCTC | 5 cut(s) | 80, 992, 999, 1261, 1421 |
| BfaI | CTAG | 2 cut(s) | 1044, 1401 |
| BfmI | CTRYAG | 1 cut(s) | 918 |
| BfuI | GTATCC | 1 cut(s) | 1253 |
| BisI | GCNGC | 7 cut(s) | 71, 354, 405, 408, 906, 918, 921 |
| BlpI | GCTNAGC | 2 cut(s) | 557, 1370 |
| BlsI | GCNGC | 7 cut(s) | 72, 355, 406, 409, 907, 919, 922 |
| BmcAI | AGTACT | 1 cut(s) | 568 |
| Bme1390I | CCNGG | 3 cut(s) | 38, 179, 703 |
| Bme18I | GGWCC | 1 cut(s) | 874 |
| BmeT110I | CYCGRG | 1 cut(s) | 1002 |
| BmgT120I | GGNCC | 1 cut(s) | 874 |
| BmiI | GGNNCC | 2 cut(s) | 43, 876 |
| BmrFI | CCNGG | 3 cut(s) | 38, 179, 703 |
| BmsI | GCATC | 6 cut(s) | 39, 202, 378, 802, 936, 1222 |
| BpiI | GAAGAC | 1 cut(s) | 630 |
| BpmI | CTGGAG | 3 cut(s) | 621, 685, 1170 |
| Bpu1102I | GCTNAGC | 2 cut(s) | 557, 1370 |
| Bpu14I | TTCGAA | 1 cut(s) | 837 |
| Bsa29I | ATCGAT | 1 cut(s) | 852 |
| BsaAI | YACGTR | 1 cut(s) | 48 |
| BsaI | GGTCTC | 1 cut(s) | 80 |
| BsaJI | CCNNGG | 4 cut(s) | 37, 372, 613, 702 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 1431 |
| Bse1I | ACTGG | 2 cut(s) | 1282, 1341 |
| BseBI | CCWGG | 3 cut(s) | 38, 179, 703 |
| BseCI | ATCGAT | 1 cut(s) | 852 |
| BseDI | CCNNGG | 4 cut(s) | 37, 372, 613, 702 |
| BseGI | GGATG | 3 cut(s) | 368, 691, 976 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 1431 |
| BseMII | CTCAG | 1 cut(s) | 1254 |
| BseNI | ACTGG | 2 cut(s) | 1282, 1341 |
| BseRI | GAGGAG | 1 cut(s) | 760 |
| BseXI | GCAGC | 7 cut(s) | 82, 340, 391, 394, 892, 904, 932 |
| Bsh1285I | CGRYCG | 1 cut(s) | 79 |
| BshFI | GGCC | 3 cut(s) | 185, 277, 336 |
| BshVI | ATCGAT | 1 cut(s) | 852 |
| BsiEI | CGRYCG | 1 cut(s) | 79 |
| BsiHKAI | GWGCWC | 1 cut(s) | 1204 |
| BsiHKCI | CYCGRG | 1 cut(s) | 1002 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 1431 |
| BsmAI | GTCTC | 5 cut(s) | 80, 992, 999, 1261, 1421 |
| BsnI | GGCC | 3 cut(s) | 185, 277, 336 |
| Bso31I | GGTCTC | 1 cut(s) | 80 |
| BsoBI | CYCGRG | 1 cut(s) | 1002 |
| Bsp119I | TTCGAA | 1 cut(s) | 837 |
| Bsp1286I | GDGCHC | 1 cut(s) | 1204 |
| Bsp1407I | TGTACA | 1 cut(s) | 1295 |
| Bsp143I | GATC | 2 cut(s) | 1029, 1407 |
| Bsp1720I | GCTNAGC | 2 cut(s) | 557, 1370 |
| Bsp19I | CCATGG | 1 cut(s) | 372 |
| BspANI | GGCC | 3 cut(s) | 185, 277, 336 |
| BspCNI | CTCAG | 1 cut(s) | 1255 |
| BspDI | ATCGAT | 1 cut(s) | 852 |
| BspHI | TCATGA | 1 cut(s) | 241 |
| BspLI | GGNNCC | 2 cut(s) | 43, 876 |
| BspMAI | CTGCAG | 1 cut(s) | 922 |
| BspPI | GGATC | 1 cut(s) | 1024 |
| BspT104I | TTCGAA | 1 cut(s) | 837 |
| BspTNI | GGTCTC | 1 cut(s) | 80 |
| BsrGI | TGTACA | 1 cut(s) | 1295 |
| BsrI | ACTGG | 2 cut(s) | 1282, 1341 |
| BssECI | CCNNGG | 4 cut(s) | 37, 372, 613, 702 |
| BssMI | GATC | 2 cut(s) | 1029, 1407 |
| BssSI | CACGAG | 1 cut(s) | 52 |
| BssT1I | CCWWGG | 2 cut(s) | 372, 613 |
| Bst2BI | CACGAG | 1 cut(s) | 52 |
| Bst2UI | CCWGG | 3 cut(s) | 38, 179, 703 |
| Bst4CI | ACNGT | 2 cut(s) | 77, 205 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 902 |
| BstAUI | TGTACA | 1 cut(s) | 1295 |
| BstBAI | YACGTR | 1 cut(s) | 48 |
| BstBI | TTCGAA | 1 cut(s) | 837 |
| BstC8I | GCNNGC | 1 cut(s) | 418 |
| BstDEI | CTNAG | 3 cut(s) | 557, 1263, 1370 |
| BstDSI | CCRYGG | 1 cut(s) | 372 |
| BstF5I | GGATG | 3 cut(s) | 368, 691, 976 |
| BstKTI | GATC | 2 cut(s) | 1032, 1410 |
| BstMAI | GTCTC | 5 cut(s) | 80, 992, 999, 1261, 1421 |
| BstMBI | GATC | 2 cut(s) | 1029, 1407 |
| BstMCI | CGRYCG | 1 cut(s) | 79 |
| BstMWI | GCNNNNNNNGC | 4 cut(s) | 159, 413, 902, 914 |
| BstNI | CCWGG | 3 cut(s) | 38, 179, 703 |
| BstNSI | RCATGY | 2 cut(s) | 23, 252 |
| BstSCI | CCNGG | 3 cut(s) | 36, 177, 701 |
| BstSFI | CTRYAG | 1 cut(s) | 918 |
| BstV1I | GCAGC | 7 cut(s) | 82, 340, 391, 394, 892, 904, 932 |
| BstV2I | GAAGAC | 1 cut(s) | 630 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 379 |
| Bsu15I | ATCGAT | 1 cut(s) | 852 |
| BsuI | GTATCC | 1 cut(s) | 1253 |
| BsuRI | GGCC | 3 cut(s) | 185, 277, 336 |
| BsuTUI | ATCGAT | 1 cut(s) | 852 |
| BtgI | CCRYGG | 1 cut(s) | 372 |
| BtgZI | GCGATG | 1 cut(s) | 177 |
| BtsCI | GGATG | 3 cut(s) | 368, 691, 976 |
| BtsI | GCAGTG | 2 cut(s) | 69, 411 |
| BtsIMutI | CAGTG | 4 cut(s) | 69, 411, 897, 1275 |
| Cac8I | GCNNGC | 1 cut(s) | 418 |
| CciI | TCATGA | 1 cut(s) | 241 |
| Cfr13I | GGNCC | 1 cut(s) | 874 |
| ClaI | ATCGAT | 1 cut(s) | 852 |
| CsiI | ACCWGGT | 1 cut(s) | 177 |
| Csp6I | GTAC | 5 cut(s) | 49, 537, 567, 1084, 1296 |
| CviAII | CATG | 8 cut(s) | 20, 242, 249, 373, 871, 1018, 1251, 1385 |
| CviQI | GTAC | 5 cut(s) | 49, 537, 567, 1084, 1296 |
| DdeI | CTNAG | 3 cut(s) | 557, 1263, 1370 |
| DpnI | GATC | 2 cut(s) | 1031, 1409 |
| DpnII | GATC | 2 cut(s) | 1029, 1407 |
| DrdI | GACNNNNNNGTC | 1 cut(s) | 121 |
| DseDI | GACNNNNNNGTC | 1 cut(s) | 121 |
| EaeI | YGGCCR | 2 cut(s) | 183, 275 |
| Ecl136II | GAGCTC | 1 cut(s) | 1202 |
| Eco130I | CCWWGG | 2 cut(s) | 372, 613 |
| Eco24I | GRGCYC | 1 cut(s) | 1204 |
| Eco31I | GGTCTC | 1 cut(s) | 80 |
| Eco47I | GGWCC | 1 cut(s) | 874 |
| Eco53kI | GAGCTC | 1 cut(s) | 1202 |
| Eco57I | CTGAAG | 1 cut(s) | 378 |
| Eco88I | CYCGRG | 1 cut(s) | 1002 |
| EcoICRI | GAGCTC | 1 cut(s) | 1202 |
| EcoRI | GAATTC | 2 cut(s) | 662, 1047 |
| EcoRII | CCWGG | 3 cut(s) | 36, 177, 701 |
| EcoT14I | CCWWGG | 2 cut(s) | 372, 613 |
| EcoT38I | GRGCYC | 1 cut(s) | 1204 |
| ErhI | CCWWGG | 2 cut(s) | 372, 613 |
| FaeI | CATG | 8 cut(s) | 23, 245, 252, 376, 874, 1021, 1254, 1388 |
| FatI | CATG | 8 cut(s) | 19, 241, 248, 372, 870, 1017, 1250, 1384 |
| FauNDI | CATATG | 1 cut(s) | 898 |
| Fnu4HI | GCNGC | 7 cut(s) | 71, 354, 405, 408, 906, 918, 921 |
| FokI | GGATG | 3 cut(s) | 355, 698, 983 |
| FriOI | GRGCYC | 1 cut(s) | 1204 |
| Fsp4HI | GCNGC | 7 cut(s) | 71, 354, 405, 408, 906, 918, 921 |
| FspBI | CTAG | 2 cut(s) | 1044, 1401 |
| GluI | GCNGC | 7 cut(s) | 71, 354, 405, 408, 906, 918, 921 |
| GsuI | CTGGAG | 3 cut(s) | 621, 685, 1170 |
| HaeIII | GGCC | 3 cut(s) | 185, 277, 336 |
| Hin1II | CATG | 8 cut(s) | 23, 245, 252, 376, 874, 1021, 1254, 1388 |
| HincII | GTYRAC | 1 cut(s) | 325 |
| HindII | GTYRAC | 1 cut(s) | 325 |
| HinfI | GANTC | 6 cut(s) | 118, 143, 430, 1000, 1129, 1337 |
| HphI | GGTGA | 7 cut(s) | 130, 187, 602, 632, 655, 967, 1292 |
| Hpy166II | GTNNAC | 4 cut(s) | 325, 646, 1275, 1296 |
| Hpy188I | TCNGA | 2 cut(s) | 258, 1194 |
| Hpy188III | TCNNGA | 2 cut(s) | 242, 1004 |
| Hpy8I | GTNNAC | 4 cut(s) | 325, 646, 1275, 1296 |
| HpyAV | CCTTC | 7 cut(s) | 145, 287, 583, 730, 1189, 1322, 1382 |
| HpyCH4III | ACNGT | 2 cut(s) | 77, 205 |
| HpyCH4IV | ACGT | 2 cut(s) | 47, 1414 |
| HpyCH4V | TGCA | 8 cut(s) | 23, 62, 215, 461, 793, 870, 920, 1011 |
| HpyF10VI | GCNNNNNNNGC | 4 cut(s) | 159, 413, 902, 914 |
| HpyF3I | CTNAG | 3 cut(s) | 557, 1263, 1370 |
| HpySE526I | ACGT | 2 cut(s) | 47, 1414 |
| Hsp92II | CATG | 8 cut(s) | 23, 245, 252, 376, 874, 1021, 1254, 1388 |
| Kzo9I | GATC | 2 cut(s) | 1029, 1407 |
| LmnI | GCTCC | 3 cut(s) | 167, 1199, 1207 |
| Lsp1109I | GCAGC | 7 cut(s) | 82, 340, 391, 394, 892, 904, 932 |
| LweI | GCATC | 6 cut(s) | 39, 202, 378, 802, 936, 1222 |
| MabI | ACCWGGT | 1 cut(s) | 177 |
| MaeI | CTAG | 2 cut(s) | 1044, 1401 |
| MaeII | ACGT | 2 cut(s) | 47, 1414 |
| MaeIII | GTNAC | 1 cut(s) | 973 |
| MalI | GATC | 2 cut(s) | 1031, 1409 |
| MboI | GATC | 2 cut(s) | 1029, 1407 |
| MboII | GAAGA | 5 cut(s) | 26, 246, 371, 635, 850 |
| MfeI | CAATTG | 1 cut(s) | 462 |
| MhlI | GDGCHC | 1 cut(s) | 1204 |
| MlsI | TGGCCA | 2 cut(s) | 185, 277 |
| MluNI | TGGCCA | 2 cut(s) | 185, 277 |
| MlyI | GAGTC | 5 cut(s) | 112, 137, 994, 1138, 1346 |
| MmeI | TCCRAC | 1 cut(s) | 1157 |
| MnlI | CCTC | 8 cut(s) | 203, 213, 652, 708, 738, 741, 1114, 1194 |
| Mox20I | TGGCCA | 2 cut(s) | 185, 277 |
| MscI | TGGCCA | 2 cut(s) | 185, 277 |
| MseI | TTAA | 3 cut(s) | 174, 719, 815 |
| MslI | CAYNNNNRTG | 3 cut(s) | 78, 615, 1058 |
| Msp20I | TGGCCA | 2 cut(s) | 185, 277 |
| MspR9I | CCNGG | 3 cut(s) | 38, 179, 703 |
| MunI | CAATTG | 1 cut(s) | 462 |
| MvaI | CCWGG | 3 cut(s) | 38, 179, 703 |
| MwoI | GCNNNNNNNGC | 4 cut(s) | 159, 413, 902, 914 |
| NcoI | CCATGG | 1 cut(s) | 372 |
| NdeI | CATATG | 1 cut(s) | 898 |
| NdeII | GATC | 2 cut(s) | 1029, 1407 |
| NlaIII | CATG | 8 cut(s) | 23, 245, 252, 376, 874, 1021, 1254, 1388 |
| NlaIV | GGNNCC | 2 cut(s) | 43, 876 |
| NmuCI | GTSAC | 1 cut(s) | 973 |
| NspI | RCATGY | 2 cut(s) | 23, 252 |
| NspV | TTCGAA | 1 cut(s) | 837 |
| OliI | CACNNNNGTG | 2 cut(s) | 78, 615 |
| PaeR7I | CTCGAG | 1 cut(s) | 1002 |
| PagI | TCATGA | 1 cut(s) | 241 |
| PciI | ACATGT | 1 cut(s) | 248 |
| PfeI | GAWTC | 1 cut(s) | 430 |
| PflFI | GACNNNGTC | 1 cut(s) | 121 |
| PkrI | GCNGC | 7 cut(s) | 72, 355, 406, 409, 907, 919, 922 |
| PleI | GAGTC | 5 cut(s) | 112, 137, 994, 1137, 1345 |
| PpsI | GAGTC | 5 cut(s) | 112, 137, 994, 1137, 1345 |
| Ppu21I | YACGTR | 1 cut(s) | 48 |
| PscI | ACATGT | 1 cut(s) | 248 |
| Psp124BI | GAGCTC | 1 cut(s) | 1204 |
| Psp6I | CCWGG | 3 cut(s) | 36, 177, 701 |
| PspGI | CCWGG | 3 cut(s) | 36, 177, 701 |
| PspN4I | GGNNCC | 2 cut(s) | 43, 876 |
| PspPI | GGNCC | 1 cut(s) | 874 |
| PstI | CTGCAG | 1 cut(s) | 922 |
| PsyI | GACNNNGTC | 1 cut(s) | 121 |
| RsaI | GTAC | 5 cut(s) | 50, 538, 568, 1085, 1297 |
| RsaNI | GTAC | 5 cut(s) | 49, 537, 567, 1084, 1296 |
| RseI | CAYNNNNRTG | 3 cut(s) | 78, 615, 1058 |
| SacI | GAGCTC | 1 cut(s) | 1204 |
| SaqAI | TTAA | 3 cut(s) | 174, 719, 815 |
| SatI | GCNGC | 7 cut(s) | 71, 354, 405, 408, 906, 918, 921 |
| Sau3AI | GATC | 2 cut(s) | 1029, 1407 |
| Sau96I | GGNCC | 1 cut(s) | 874 |
| ScaI | AGTACT | 1 cut(s) | 568 |
| SchI | GAGTC | 5 cut(s) | 112, 137, 994, 1138, 1346 |
| ScrFI | CCNGG | 3 cut(s) | 38, 179, 703 |
| SduI | GDGCHC | 1 cut(s) | 1204 |
| SexAI | ACCWGGT | 1 cut(s) | 177 |
| SfaNI | GCATC | 6 cut(s) | 39, 202, 378, 802, 936, 1222 |
| SfcI | CTRYAG | 1 cut(s) | 918 |
| Sfr274I | CTCGAG | 1 cut(s) | 1002 |
| SfuI | TTCGAA | 1 cut(s) | 837 |
| SinI | GGWCC | 1 cut(s) | 874 |
| SlaI | CTCGAG | 1 cut(s) | 1002 |
| SmiMI | CAYNNNNRTG | 3 cut(s) | 78, 615, 1058 |
| SmlI | CTYRAG | 1 cut(s) | 1002 |
| SmoI | CTYRAG | 1 cut(s) | 1002 |
| SspMI | CTAG | 2 cut(s) | 1044, 1401 |
| SstI | GAGCTC | 1 cut(s) | 1204 |
| StyD4I | CCNGG | 3 cut(s) | 36, 177, 701 |
| StyI | CCWWGG | 2 cut(s) | 372, 613 |
| TaaI | ACNGT | 2 cut(s) | 77, 205 |
| TaiI | ACGT | 2 cut(s) | 50, 1417 |
| TaqI | TCGA | 6 cut(s) | 33, 630, 837, 852, 1003, 1119 |
| TatI | WGTACW | 2 cut(s) | 566, 1295 |
| TfiI | GAWTC | 1 cut(s) | 430 |
| Tru1I | TTAA | 3 cut(s) | 174, 719, 815 |
| Tru9I | TTAA | 3 cut(s) | 174, 719, 815 |
| TscAI | CASTG | 4 cut(s) | 69, 418, 897, 1282 |
| TseFI | GTSAC | 1 cut(s) | 973 |
| TseI | GCWGC | 7 cut(s) | 70, 353, 404, 407, 905, 917, 920 |
| Tsp45I | GTSAC | 1 cut(s) | 973 |
| TspGWI | ACGGA | 2 cut(s) | 111, 136 |
| TspRI | CASTG | 4 cut(s) | 69, 418, 897, 1282 |
| Tth111I | GACNNNGTC | 1 cut(s) | 121 |
| VpaK11BI | GGWCC | 1 cut(s) | 874 |
| XapI | RAATTY | 5 cut(s) | 363, 662, 833, 1047, 1163 |
| XceI | RCATGY | 2 cut(s) | 23, 252 |
| XhoI | CTCGAG | 1 cut(s) | 1002 |
| XspI | CTAG | 2 cut(s) | 1044, 1401 |
| ZrmI | AGTACT | 1 cut(s) | 568 |