Belongs to the heat shock protein 70 family
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AatII | GACGTC | 1 cut(s) | 594 |
| AccB1I | GGYRCC | 1 cut(s) | 950 |
| AccI | GTMKAC | 1 cut(s) | 668 |
| AciI | CCGC | 3 cut(s) | 40, 954, 1268 |
| AclWI | GGATC | 1 cut(s) | 244 |
| AcoI | YGGCCR | 1 cut(s) | 95 |
| AcsI | RAATTY | 2 cut(s) | 737, 1119 |
| AcuI | CTGAAG | 2 cut(s) | 198, 243 |
| AcyI | GRCGYC | 1 cut(s) | 591 |
| AdeI | CACNNNGTG | 1 cut(s) | 950 |
| AfaI | GTAC | 3 cut(s) | 462, 873, 990 |
| AflII | CTTAAG | 2 cut(s) | 26, 1237 |
| AjnI | CCWGG | 1 cut(s) | 991 |
| AjuI | GAANNNNNNNTTGG | 4 cut(s) | 216, 248, 569, 601 |
| AluBI | AGCT | 8 cut(s) | 91, 227, 611, 910, 1000, 1179, 1236, 1261 |
| AluI | AGCT | 8 cut(s) | 91, 227, 611, 910, 1000, 1179, 1236, 1261 |
| Alw21I | GWGCWC | 1 cut(s) | 423 |
| AlwI | GGATC | 1 cut(s) | 244 |
| Ama87I | CYCGRG | 1 cut(s) | 1114 |
| AoxI | GGCC | 3 cut(s) | 95, 325, 379 |
| ApeKI | GCWGC | 4 cut(s) | 173, 362, 365, 965 |
| ApoI | RAATTY | 2 cut(s) | 737, 1119 |
| Asp700I | GAANNNNTTC | 2 cut(s) | 655, 893 |
| AspS9I | GGNCC | 1 cut(s) | 116 |
| AsuHPI | GGTGA | 5 cut(s) | 346, 536, 913, 1018, 1208 |
| AvaI | CYCGRG | 1 cut(s) | 1114 |
| AvaII | GGWCC | 1 cut(s) | 116 |
| AxyI | CCTNAGG | 1 cut(s) | 1127 |
| BalI | TGGCCA | 1 cut(s) | 97 |
| BanI | GGYRCC | 1 cut(s) | 950 |
| BbsI | GAAGAC | 3 cut(s) | 534, 658, 846 |
| Bbv12I | GWGCWC | 1 cut(s) | 423 |
| BbvI | GCAGC | 4 cut(s) | 160, 349, 377, 952 |
| BccI | CCATC | 3 cut(s) | 470, 1064, 1133 |
| BceAI | ACGGC | 1 cut(s) | 1069 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 341, 375 |
| BciT130I | CCWGG | 1 cut(s) | 993 |
| BfaI | CTAG | 2 cut(s) | 120, 1265 |
| BfmI | CTRYAG | 4 cut(s) | 363, 501, 939, 966 |
| BfrI | CTTAAG | 2 cut(s) | 26, 1237 |
| BisI | GCNGC | 5 cut(s) | 174, 363, 366, 954, 966 |
| BlsI | GCNGC | 5 cut(s) | 175, 364, 367, 955, 967 |
| BmcAI | AGTACT | 1 cut(s) | 462 |
| Bme1390I | CCNGG | 1 cut(s) | 993 |
| Bme18I | GGWCC | 1 cut(s) | 116 |
| BmeT110I | CYCGRG | 1 cut(s) | 1114 |
| BmgT120I | GGNCC | 1 cut(s) | 116 |
| BmiI | GGNNCC | 3 cut(s) | 780, 952, 1083 |
| BmrFI | CCNGG | 1 cut(s) | 993 |
| BmsI | GCATC | 5 cut(s) | 198, 327, 706, 793, 1293 |
| BpiI | GAAGAC | 3 cut(s) | 534, 658, 846 |
| BpmI | CTGGAG | 1 cut(s) | 1241 |
| BsaHI | GRCGYC | 1 cut(s) | 591 |
| BsaJI | CCNNGG | 5 cut(s) | 69, 232, 517, 867, 992 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 46, 76 |
| Bse21I | CCTNAGG | 1 cut(s) | 1127 |
| Bse3DI | GCAATG | 3 cut(s) | 369, 829, 1250 |
| BseBI | CCWGG | 1 cut(s) | 993 |
| BseDI | CCNNGG | 5 cut(s) | 69, 232, 517, 867, 992 |
| BseGI | GGATG | 4 cut(s) | 481, 808, 835, 1027 |
| BseMI | GCAATG | 3 cut(s) | 369, 829, 1250 |
| BseXI | GCAGC | 4 cut(s) | 160, 349, 377, 952 |
| BshFI | GGCC | 3 cut(s) | 97, 327, 381 |
| BshNI | GGYRCC | 1 cut(s) | 950 |
| BsiHKAI | GWGCWC | 1 cut(s) | 423 |
| BsiHKCI | CYCGRG | 1 cut(s) | 1114 |
| BslFI | GGGAC | 2 cut(s) | 251, 457 |
| BsmFI | GGGAC | 2 cut(s) | 251, 457 |
| BsnI | GGCC | 3 cut(s) | 97, 327, 381 |
| BsoBI | CYCGRG | 1 cut(s) | 1114 |
| Bsp1286I | GDGCHC | 1 cut(s) | 423 |
| Bsp143I | GATC | 1 cut(s) | 236 |
| BspACI | CCGC | 3 cut(s) | 40, 954, 1268 |
| BspANI | GGCC | 3 cut(s) | 97, 327, 381 |
| BspHI | TCATGA | 2 cut(s) | 765, 1089 |
| BspLI | GGNNCC | 3 cut(s) | 780, 952, 1083 |
| BspMAI | CTGCAG | 3 cut(s) | 367, 505, 970 |
| BspPI | GGATC | 1 cut(s) | 244 |
| BspT107I | GGYRCC | 1 cut(s) | 950 |
| BspTI | CTTAAG | 2 cut(s) | 26, 1237 |
| BsrDI | GCAATG | 3 cut(s) | 369, 829, 1250 |
| BssECI | CCNNGG | 5 cut(s) | 69, 232, 517, 867, 992 |
| BssMI | GATC | 1 cut(s) | 236 |
| BssNI | GRCGYC | 1 cut(s) | 591 |
| BssT1I | CCWWGG | 4 cut(s) | 69, 232, 517, 867 |
| Bst2UI | CCWGG | 1 cut(s) | 993 |
| Bst4CI | ACNGT | 3 cut(s) | 244, 265, 1153 |
| Bst6I | CTCTTC | 1 cut(s) | 1127 |
| BstACI | GRCGYC | 1 cut(s) | 591 |
| BstAFI | CTTAAG | 2 cut(s) | 26, 1237 |
| BstAPI | GCANNNNNTGC | 2 cut(s) | 371, 950 |
| BstC8I | GCNNGC | 3 cut(s) | 313, 325, 397 |
| BstDEI | CTNAG | 2 cut(s) | 614, 1127 |
| BstEII | GGTNACC | 1 cut(s) | 142 |
| BstF5I | GGATG | 4 cut(s) | 481, 808, 835, 1027 |
| BstKTI | GATC | 1 cut(s) | 239 |
| BstMBI | GATC | 1 cut(s) | 236 |
| BstMWI | GCNNNNNNNGC | 4 cut(s) | 371, 405, 950, 962 |
| BstNI | CCWGG | 1 cut(s) | 993 |
| BstNSI | RCATGY | 1 cut(s) | 311 |
| BstPI | GGTNACC | 1 cut(s) | 142 |
| BstSCI | CCNGG | 1 cut(s) | 991 |
| BstSFI | CTRYAG | 4 cut(s) | 363, 501, 939, 966 |
| BstV1I | GCAGC | 4 cut(s) | 160, 349, 377, 952 |
| BstV2I | GAAGAC | 3 cut(s) | 534, 658, 846 |
| Bsu36I | CCTNAGG | 1 cut(s) | 1127 |
| BsuRI | GGCC | 3 cut(s) | 97, 327, 381 |
| BtsCI | GGATG | 4 cut(s) | 481, 808, 835, 1027 |
| BtsI | GCAGTG | 1 cut(s) | 498 |
| BtsIMutI | CAGTG | 4 cut(s) | 67, 261, 498, 601 |
| Cac8I | GCNNGC | 3 cut(s) | 313, 325, 397 |
| CciI | TCATGA | 2 cut(s) | 765, 1089 |
| Cfr13I | GGNCC | 1 cut(s) | 116 |
| CseI | GACGC | 1 cut(s) | 547 |
| Csp6I | GTAC | 3 cut(s) | 461, 872, 989 |
| CviAII | CATG | 7 cut(s) | 85, 308, 581, 766, 898, 947, 1090 |
| CviQI | GTAC | 3 cut(s) | 461, 872, 989 |
| DdeI | CTNAG | 2 cut(s) | 614, 1127 |
| DpnI | GATC | 1 cut(s) | 238 |
| DpnII | GATC | 1 cut(s) | 236 |
| DraI | TTTAAA | 1 cut(s) | 742 |
| DraIII | CACNNNGTG | 1 cut(s) | 950 |
| EaeI | YGGCCR | 1 cut(s) | 95 |
| Eam1104I | CTCTTC | 1 cut(s) | 1127 |
| EarI | CTCTTC | 1 cut(s) | 1127 |
| Eco130I | CCWWGG | 4 cut(s) | 69, 232, 517, 867 |
| Eco47I | GGWCC | 1 cut(s) | 116 |
| Eco57I | CTGAAG | 2 cut(s) | 198, 243 |
| Eco81I | CCTNAGG | 1 cut(s) | 1127 |
| Eco88I | CYCGRG | 1 cut(s) | 1114 |
| Eco91I | GGTNACC | 1 cut(s) | 142 |
| EcoO109I | RGGNCCY | 1 cut(s) | 116 |
| EcoO65I | GGTNACC | 1 cut(s) | 142 |
| EcoRI | GAATTC | 1 cut(s) | 1119 |
| EcoRII | CCWGG | 1 cut(s) | 991 |
| EcoT14I | CCWWGG | 4 cut(s) | 69, 232, 517, 867 |
| EcoT22I | ATGCAT | 1 cut(s) | 342 |
| ErhI | CCWWGG | 4 cut(s) | 69, 232, 517, 867 |
| FaeI | CATG | 7 cut(s) | 88, 311, 584, 769, 901, 950, 1093 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 216, 248 |
| FaqI | GGGAC | 2 cut(s) | 251, 457 |
| FatI | CATG | 7 cut(s) | 84, 307, 580, 765, 897, 946, 1089 |
| FblI | GTMKAC | 1 cut(s) | 668 |
| Fnu4HI | GCNGC | 5 cut(s) | 174, 363, 366, 954, 966 |
| FokI | GGATG | 4 cut(s) | 488, 815, 842, 1034 |
| Fsp4HI | GCNGC | 5 cut(s) | 174, 363, 366, 954, 966 |
| FspBI | CTAG | 2 cut(s) | 120, 1265 |
| GluI | GCNGC | 5 cut(s) | 174, 363, 366, 954, 966 |
| GsuI | CTGGAG | 1 cut(s) | 1241 |
| HaeIII | GGCC | 3 cut(s) | 97, 327, 381 |
| HgaI | GACGC | 1 cut(s) | 547 |
| Hin1I | GRCGYC | 1 cut(s) | 591 |
| Hin1II | CATG | 7 cut(s) | 88, 311, 584, 769, 901, 950, 1093 |
| HincII | GTYRAC | 1 cut(s) | 550 |
| HindII | GTYRAC | 1 cut(s) | 550 |
| HindIII | AAGCTT | 3 cut(s) | 225, 998, 1234 |
| HinfI | GANTC | 5 cut(s) | 32, 57, 861, 1102, 1280 |
| HpaI | GTTAAC | 1 cut(s) | 550 |
| HphI | GGTGA | 5 cut(s) | 346, 536, 913, 1018, 1208 |
| Hpy166II | GTNNAC | 2 cut(s) | 550, 669 |
| Hpy188I | TCNGA | 1 cut(s) | 135 |
| Hpy188III | TCNNGA | 9 cut(s) | 571, 766, 858, 929, 1004, 1090, 1099, 1116, 1317 |
| Hpy8I | GTNNAC | 2 cut(s) | 550, 669 |
| Hpy99I | CGWCG | 1 cut(s) | 563 |
| HpyCH4III | ACNGT | 3 cut(s) | 244, 265, 1153 |
| HpyCH4IV | ACGT | 4 cut(s) | 292, 591, 665, 678 |
| HpyCH4V | TGCA | 7 cut(s) | 203, 340, 365, 503, 697, 915, 968 |
| HpyF10VI | GCNNNNNNNGC | 4 cut(s) | 371, 405, 950, 962 |
| HpyF3I | CTNAG | 2 cut(s) | 614, 1127 |
| HpySE526I | ACGT | 4 cut(s) | 292, 591, 665, 678 |
| Hsp92I | GRCGYC | 1 cut(s) | 591 |
| Hsp92II | CATG | 7 cut(s) | 88, 311, 584, 769, 901, 950, 1093 |
| KspAI | GTTAAC | 1 cut(s) | 550 |
| Kzo9I | GATC | 1 cut(s) | 236 |
| LmnI | GCTCC | 4 cut(s) | 778, 1081, 1260, 1266 |
| Lsp1109I | GCAGC | 4 cut(s) | 160, 349, 377, 952 |
| LweI | GCATC | 5 cut(s) | 198, 327, 706, 793, 1293 |
| MaeI | CTAG | 2 cut(s) | 120, 1265 |
| MaeII | ACGT | 4 cut(s) | 292, 591, 665, 678 |
| MaeIII | GTNAC | 8 cut(s) | 62, 80, 142, 718, 1024, 1035, 1144, 1157 |
| MalI | GATC | 1 cut(s) | 238 |
| MboI | GATC | 1 cut(s) | 236 |
| MboII | GAAGA | 6 cut(s) | 191, 539, 663, 754, 846, 1144 |
| MhlI | GDGCHC | 1 cut(s) | 423 |
| MlsI | TGGCCA | 1 cut(s) | 97 |
| MluCI | AATT | 7 cut(s) | 45, 108, 216, 566, 684, 737, 1119 |
| MluNI | TGGCCA | 1 cut(s) | 97 |
| Mox20I | TGGCCA | 1 cut(s) | 97 |
| Mph1103I | ATGCAT | 1 cut(s) | 342 |
| MroXI | GAANNNNTTC | 2 cut(s) | 655, 893 |
| MscI | TGGCCA | 1 cut(s) | 97 |
| MseI | TTAA | 7 cut(s) | 27, 44, 348, 549, 623, 741, 1238 |
| Msp20I | TGGCCA | 1 cut(s) | 97 |
| MspA1I | CMGCKG | 1 cut(s) | 956 |
| MspCI | CTTAAG | 2 cut(s) | 26, 1237 |
| MspR9I | CCNGG | 1 cut(s) | 993 |
| MvaI | CCWGG | 1 cut(s) | 993 |
| MwoI | GCNNNNNNNGC | 4 cut(s) | 371, 405, 950, 962 |
| NdeII | GATC | 1 cut(s) | 236 |
| NlaIII | CATG | 7 cut(s) | 88, 311, 584, 769, 901, 950, 1093 |
| NlaIV | GGNNCC | 3 cut(s) | 780, 952, 1083 |
| NmuCI | GTSAC | 5 cut(s) | 62, 80, 1024, 1035, 1157 |
| NsiI | ATGCAT | 1 cut(s) | 342 |
| NspI | RCATGY | 1 cut(s) | 311 |
| PaeR7I | CTCGAG | 1 cut(s) | 1114 |
| PagI | TCATGA | 2 cut(s) | 765, 1089 |
| PdmI | GAANNNNTTC | 2 cut(s) | 655, 893 |
| PfeI | GAWTC | 5 cut(s) | 32, 57, 861, 1102, 1280 |
| PkrI | GCNGC | 5 cut(s) | 175, 364, 367, 955, 967 |
| PpuMI | RGGWCCY | 1 cut(s) | 116 |
| Psp5II | RGGWCCY | 1 cut(s) | 116 |
| Psp6I | CCWGG | 1 cut(s) | 991 |
| PspEI | GGTNACC | 1 cut(s) | 142 |
| PspGI | CCWGG | 1 cut(s) | 991 |
| PspN4I | GGNNCC | 3 cut(s) | 780, 952, 1083 |
| PspPI | GGNCC | 1 cut(s) | 116 |
| PspPPI | RGGWCCY | 1 cut(s) | 116 |
| PstI | CTGCAG | 3 cut(s) | 367, 505, 970 |
| RsaI | GTAC | 3 cut(s) | 462, 873, 990 |
| RsaNI | GTAC | 3 cut(s) | 461, 872, 989 |
| SaqAI | TTAA | 7 cut(s) | 27, 44, 348, 549, 623, 741, 1238 |
| SatI | GCNGC | 5 cut(s) | 174, 363, 366, 954, 966 |
| Sau3AI | GATC | 1 cut(s) | 236 |
| Sau96I | GGNCC | 1 cut(s) | 116 |
| ScaI | AGTACT | 1 cut(s) | 462 |
| ScrFI | CCNGG | 1 cut(s) | 993 |
| SduI | GDGCHC | 1 cut(s) | 423 |
| SfaNI | GCATC | 5 cut(s) | 198, 327, 706, 793, 1293 |
| SfcI | CTRYAG | 4 cut(s) | 363, 501, 939, 966 |
| Sfr274I | CTCGAG | 1 cut(s) | 1114 |
| SinI | GGWCC | 1 cut(s) | 116 |
| SlaI | CTCGAG | 1 cut(s) | 1114 |
| SmlI | CTYRAG | 3 cut(s) | 26, 1114, 1237 |
| SmoI | CTYRAG | 3 cut(s) | 26, 1114, 1237 |
| Sse9I | AATT | 7 cut(s) | 45, 108, 216, 566, 684, 737, 1119 |
| SsiI | CCGC | 3 cut(s) | 40, 954, 1268 |
| SspMI | CTAG | 2 cut(s) | 120, 1265 |
| StyD4I | CCNGG | 1 cut(s) | 991 |
| StyI | CCWWGG | 4 cut(s) | 69, 232, 517, 867 |
| TaaI | ACNGT | 3 cut(s) | 244, 265, 1153 |
| TaiI | ACGT | 4 cut(s) | 295, 594, 668, 681 |
| TaqI | TCGA | 3 cut(s) | 561, 859, 1115 |
| TasI | AATT | 7 cut(s) | 45, 108, 216, 566, 684, 737, 1119 |
| TatI | WGTACW | 1 cut(s) | 460 |
| TauI | GCSGC | 1 cut(s) | 956 |
| TfiI | GAWTC | 5 cut(s) | 32, 57, 861, 1102, 1280 |
| Tru1I | TTAA | 7 cut(s) | 27, 44, 348, 549, 623, 741, 1238 |
| Tru9I | TTAA | 7 cut(s) | 27, 44, 348, 549, 623, 741, 1238 |
| TscAI | CASTG | 4 cut(s) | 67, 268, 505, 601 |
| TseFI | GTSAC | 5 cut(s) | 62, 80, 1024, 1035, 1157 |
| TseI | GCWGC | 4 cut(s) | 173, 362, 365, 965 |
| Tsp45I | GTSAC | 5 cut(s) | 62, 80, 1024, 1035, 1157 |
| TspDTI | ATGAA | 8 cut(s) | 33, 101, 648, 754, 782, 886, 1078, 1106 |
| TspRI | CASTG | 4 cut(s) | 67, 268, 505, 601 |
| Vha464I | CTTAAG | 2 cut(s) | 26, 1237 |
| VpaK11BI | GGWCC | 1 cut(s) | 116 |
| XapI | RAATTY | 2 cut(s) | 737, 1119 |
| XceI | RCATGY | 1 cut(s) | 311 |
| XhoI | CTCGAG | 1 cut(s) | 1114 |
| XmiI | GTMKAC | 1 cut(s) | 668 |
| XmnI | GAANNNNTTC | 2 cut(s) | 655, 893 |
| XspI | CTAG | 2 cut(s) | 120, 1265 |
| ZraI | GACGTC | 1 cut(s) | 592 |
| ZrmI | AGTACT | 1 cut(s) | 462 |
| Zsp2I | ATGCAT | 1 cut(s) | 342 |