Heat shock cognate 70 kDa
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 2 cut(s) | 443, 902 |
| Acc16I | TGCGCA | 1 cut(s) | 524 |
| Acc36I | ACCTGC | 2 cut(s) | 497, 1219 |
| AccB1I | GGYRCC | 1 cut(s) | 299 |
| AccI | GTMKAC | 1 cut(s) | 1499 |
| AccIII | TCCGGA | 2 cut(s) | 5, 1111 |
| AciI | CCGC | 8 cut(s) | 65, 339, 394, 437, 453, 548, 1121, 1485 |
| AclWI | GGATC | 1 cut(s) | 888 |
| AcoI | YGGCCR | 2 cut(s) | 278, 549 |
| AcsI | RAATTY | 3 cut(s) | 920, 1044, 1391 |
| AcuI | CTGAAG | 1 cut(s) | 381 |
| AdeI | CACNNNGTG | 1 cut(s) | 476 |
| AfaI | GTAC | 1 cut(s) | 889 |
| AfeI | AGCGCT | 1 cut(s) | 224 |
| AflIII | ACRYGT | 1 cut(s) | 82 |
| AgeI | ACCGGT | 1 cut(s) | 1502 |
| AjnI | CCWGG | 2 cut(s) | 39, 333 |
| AloI | GAACNNNNNNTCC | 2 cut(s) | 1125, 1157 |
| AluBI | AGCT | 8 cut(s) | 274, 359, 404, 1093, 1136, 1335, 1418, 1569 |
| AluI | AGCT | 8 cut(s) | 274, 359, 404, 1093, 1136, 1335, 1418, 1569 |
| Alw26I | GTCTC | 3 cut(s) | 684, 829, 1219 |
| AlwI | GGATC | 1 cut(s) | 888 |
| Aor13HI | TCCGGA | 2 cut(s) | 5, 1111 |
| Aor51HI | AGCGCT | 1 cut(s) | 224 |
| AoxI | GGCC | 5 cut(s) | 278, 549, 562, 576, 678 |
| ApeKI | GCWGC | 6 cut(s) | 73, 170, 356, 545, 1136, 1148 |
| ApoI | RAATTY | 3 cut(s) | 920, 1044, 1391 |
| AseI | ATTAAT | 1 cut(s) | 1107 |
| AsiGI | ACCGGT | 1 cut(s) | 1502 |
| Asp700I | GAANNNNTTC | 2 cut(s) | 18, 1076 |
| AspLEI | GCGC | 2 cut(s) | 225, 525 |
| AspS9I | GGNCC | 1 cut(s) | 117 |
| AsuHPI | GGTGA | 4 cut(s) | 529, 719, 1364, 1400 |
| AvaII | GGWCC | 1 cut(s) | 117 |
| AxyI | CCTNAGG | 1 cut(s) | 1422 |
| BaeI | ACNNNNGTAYC | 4 cut(s) | 240, 273, 1453, 1486 |
| BalI | TGGCCA | 1 cut(s) | 280 |
| BanI | GGYRCC | 1 cut(s) | 299 |
| BauI | CACGAG | 2 cut(s) | 55, 912 |
| BbsI | GAAGAC | 2 cut(s) | 717, 1069 |
| BbvCI | CCTCAGC | 1 cut(s) | 1488 |
| BbvI | GCAGC | 6 cut(s) | 85, 182, 368, 557, 1123, 1135 |
| BccI | CCATC | 3 cut(s) | 133, 653, 1166 |
| BciT130I | CCWGG | 2 cut(s) | 41, 335 |
| BclI | TGATCA | 2 cut(s) | 103, 318 |
| BcoDI | GTCTC | 3 cut(s) | 684, 829, 1219 |
| BfaI | CTAG | 1 cut(s) | 1041 |
| BfmI | CTRYAG | 2 cut(s) | 1281, 1294 |
| BfoI | RGCGCY | 1 cut(s) | 226 |
| BfuAI | ACCTGC | 2 cut(s) | 497, 1219 |
| BisI | GCNGC | 7 cut(s) | 74, 171, 357, 546, 549, 1137, 1149 |
| BlsI | GCNGC | 7 cut(s) | 75, 172, 358, 547, 550, 1138, 1150 |
| Bme1390I | CCNGG | 2 cut(s) | 41, 335 |
| Bme18I | GGWCC | 1 cut(s) | 117 |
| BmgT120I | GGNCC | 1 cut(s) | 117 |
| BmiI | GGNNCC | 2 cut(s) | 301, 963 |
| BmrFI | CCNGG | 2 cut(s) | 41, 335 |
| BmsI | GCATC | 7 cut(s) | 157, 205, 381, 510, 706, 976, 1192 |
| BpiI | GAAGAC | 2 cut(s) | 717, 1069 |
| BpmI | CTGGAG | 3 cut(s) | 516, 708, 1398 |
| Bpu10I | CCTNAGC | 1 cut(s) | 1488 |
| BpuEI | CTTGAG | 2 cut(s) | 587, 1495 |
| Bsa29I | ATCGAT | 2 cut(s) | 717, 1458 |
| BsaJI | CCNNGG | 4 cut(s) | 40, 334, 789, 1050 |
| BsaWI | WCCGGW | 3 cut(s) | 5, 1111, 1502 |
| BsaXI | ACNNNNNCTCC | 6 cut(s) | 229, 259, 1125, 1155, 1215, 1245 |
| Bse118I | RCCGGY | 3 cut(s) | 578, 964, 1502 |
| Bse21I | CCTNAGG | 1 cut(s) | 1422 |
| Bse3DI | GCAATG | 1 cut(s) | 552 |
| BseAI | TCCGGA | 2 cut(s) | 5, 1111 |
| BseBI | CCWGG | 2 cut(s) | 41, 335 |
| BseCI | ATCGAT | 2 cut(s) | 717, 1458 |
| BseDI | CCNNGG | 4 cut(s) | 40, 334, 789, 1050 |
| BseGI | GGATG | 4 cut(s) | 664, 778, 991, 1120 |
| BseMI | GCAATG | 1 cut(s) | 552 |
| BseMII | CTCAG | 3 cut(s) | 141, 1148, 1479 |
| BseXI | GCAGC | 6 cut(s) | 85, 182, 368, 557, 1123, 1135 |
| BsgI | GTGCAG | 3 cut(s) | 1076, 1134, 1247 |
| BshFI | GGCC | 5 cut(s) | 280, 551, 564, 578, 680 |
| BshNI | GGYRCC | 1 cut(s) | 299 |
| BshTI | ACCGGT | 1 cut(s) | 1502 |
| BshVI | ATCGAT | 2 cut(s) | 717, 1458 |
| BsiSI | CCGG | 5 cut(s) | 6, 579, 965, 1112, 1503 |
| BslFI | GGGAC | 1 cut(s) | 58 |
| BsmAI | GTCTC | 3 cut(s) | 684, 829, 1219 |
| BsmFI | GGGAC | 1 cut(s) | 58 |
| BsnI | GGCC | 5 cut(s) | 280, 551, 564, 578, 680 |
| Bsp13I | TCCGGA | 2 cut(s) | 5, 1111 |
| Bsp143I | GATC | 6 cut(s) | 103, 159, 183, 318, 745, 880 |
| BspACI | CCGC | 8 cut(s) | 65, 339, 394, 437, 453, 548, 1121, 1485 |
| BspANI | GGCC | 5 cut(s) | 280, 551, 564, 578, 680 |
| BspCNI | CTCAG | 3 cut(s) | 142, 1149, 1480 |
| BspDI | ATCGAT | 2 cut(s) | 717, 1458 |
| BspEI | TCCGGA | 2 cut(s) | 5, 1111 |
| BspHI | TCATGA | 1 cut(s) | 1012 |
| BspLI | GGNNCC | 2 cut(s) | 301, 963 |
| BspMAI | CTGCAG | 1 cut(s) | 1298 |
| BspMI | ACCTGC | 2 cut(s) | 497, 1219 |
| BspPI | GGATC | 1 cut(s) | 888 |
| BspQI | GCTCTTC | 1 cut(s) | 1042 |
| BspT107I | GGYRCC | 1 cut(s) | 299 |
| BsrDI | GCAATG | 1 cut(s) | 552 |
| BsrFI | RCCGGY | 3 cut(s) | 578, 964, 1502 |
| BssAI | RCCGGY | 3 cut(s) | 578, 964, 1502 |
| BssECI | CCNNGG | 4 cut(s) | 40, 334, 789, 1050 |
| BssMI | GATC | 6 cut(s) | 103, 159, 183, 318, 745, 880 |
| BssSI | CACGAG | 2 cut(s) | 55, 912 |
| BssT1I | CCWWGG | 2 cut(s) | 789, 1050 |
| Bst2BI | CACGAG | 2 cut(s) | 55, 912 |
| Bst2UI | CCWGG | 2 cut(s) | 41, 335 |
| Bst4CI | ACNGT | 5 cut(s) | 427, 448, 725, 1469, 1532 |
| Bst6I | CTCTTC | 1 cut(s) | 1042 |
| BstDEI | CTNAG | 5 cut(s) | 150, 797, 1157, 1422, 1488 |
| BstF5I | GGATG | 4 cut(s) | 664, 778, 991, 1120 |
| BstH2I | RGCGCY | 1 cut(s) | 226 |
| BstHHI | GCGC | 2 cut(s) | 225, 525 |
| BstKTI | GATC | 6 cut(s) | 106, 162, 186, 321, 748, 883 |
| BstMAI | GTCTC | 3 cut(s) | 684, 829, 1219 |
| BstMBI | GATC | 6 cut(s) | 103, 159, 183, 318, 745, 880 |
| BstMWI | GCNNNNNNNGC | 3 cut(s) | 554, 1133, 1145 |
| BstNI | CCWGG | 2 cut(s) | 41, 335 |
| BstNSI | RCATGY | 1 cut(s) | 86 |
| BstSCI | CCNGG | 2 cut(s) | 39, 333 |
| BstSFI | CTRYAG | 2 cut(s) | 1281, 1294 |
| BstV1I | GCAGC | 6 cut(s) | 85, 182, 368, 557, 1123, 1135 |
| BstV2I | GAAGAC | 2 cut(s) | 717, 1069 |
| Bsu15I | ATCGAT | 2 cut(s) | 717, 1458 |
| Bsu36I | CCTNAGG | 1 cut(s) | 1422 |
| BsuRI | GGCC | 5 cut(s) | 280, 551, 564, 578, 680 |
| BsuTUI | ATCGAT | 2 cut(s) | 717, 1458 |
| BtgZI | GCGATG | 1 cut(s) | 180 |
| BtsCI | GGATG | 4 cut(s) | 664, 778, 991, 1120 |
| BtsI | GCAGTG | 1 cut(s) | 681 |
| BtsIMutI | CAGTG | 4 cut(s) | 141, 250, 681, 784 |
| BveI | ACCTGC | 2 cut(s) | 497, 1219 |
| CciI | TCATGA | 1 cut(s) | 1012 |
| CfoI | GCGC | 2 cut(s) | 225, 525 |
| Cfr10I | RCCGGY | 3 cut(s) | 578, 964, 1502 |
| Cfr13I | GGNCC | 1 cut(s) | 117 |
| ClaI | ATCGAT | 2 cut(s) | 717, 1458 |
| Csp6I | GTAC | 1 cut(s) | 888 |
| CspAI | ACCGGT | 1 cut(s) | 1502 |
| CviAII | CATG | 7 cut(s) | 23, 83, 1013, 1130, 1145, 1246, 1313 |
| CviQI | GTAC | 1 cut(s) | 888 |
| DdeI | CTNAG | 5 cut(s) | 150, 797, 1157, 1422, 1488 |
| DpnI | GATC | 6 cut(s) | 105, 161, 185, 320, 747, 882 |
| DpnII | GATC | 6 cut(s) | 103, 159, 183, 318, 745, 880 |
| DraIII | CACNNNGTG | 1 cut(s) | 476 |
| EaeI | YGGCCR | 2 cut(s) | 278, 549 |
| Eam1104I | CTCTTC | 1 cut(s) | 1042 |
| EarI | CTCTTC | 1 cut(s) | 1042 |
| EciI | GGCGGA | 1 cut(s) | 354 |
| Eco130I | CCWWGG | 2 cut(s) | 789, 1050 |
| Eco47I | GGWCC | 1 cut(s) | 117 |
| Eco47III | AGCGCT | 1 cut(s) | 224 |
| Eco57I | CTGAAG | 1 cut(s) | 381 |
| Eco81I | CCTNAGG | 1 cut(s) | 1422 |
| EcoRI | GAATTC | 1 cut(s) | 1044 |
| EcoRII | CCWGG | 2 cut(s) | 39, 333 |
| EcoT14I | CCWWGG | 2 cut(s) | 789, 1050 |
| ErhI | CCWWGG | 2 cut(s) | 789, 1050 |
| FaeI | CATG | 7 cut(s) | 26, 86, 1016, 1133, 1148, 1249, 1316 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 799, 831 |
| FaqI | GGGAC | 1 cut(s) | 58 |
| FatI | CATG | 7 cut(s) | 22, 82, 1012, 1129, 1144, 1245, 1312 |
| FauI | CCCGC | 2 cut(s) | 387, 460 |
| FbaI | TGATCA | 2 cut(s) | 103, 318 |
| FblI | GTMKAC | 1 cut(s) | 1499 |
| Fnu4HI | GCNGC | 7 cut(s) | 74, 171, 357, 546, 549, 1137, 1149 |
| FokI | GGATG | 4 cut(s) | 671, 785, 998, 1127 |
| Fsp4HI | GCNGC | 7 cut(s) | 74, 171, 357, 546, 549, 1137, 1149 |
| FspAI | RTGCGCAY | 1 cut(s) | 524 |
| FspBI | CTAG | 1 cut(s) | 1041 |
| FspI | TGCGCA | 1 cut(s) | 524 |
| GlaI | GCGC | 2 cut(s) | 224, 524 |
| GluI | GCNGC | 7 cut(s) | 74, 171, 357, 546, 549, 1137, 1149 |
| GsuI | CTGGAG | 3 cut(s) | 516, 708, 1398 |
| HaeII | RGCGCY | 1 cut(s) | 226 |
| HaeIII | GGCC | 5 cut(s) | 280, 551, 564, 578, 680 |
| HapII | CCGG | 5 cut(s) | 6, 579, 965, 1112, 1503 |
| HhaI | GCGC | 2 cut(s) | 225, 525 |
| Hin1II | CATG | 7 cut(s) | 26, 86, 1016, 1133, 1148, 1249, 1316 |
| Hin6I | GCGC | 2 cut(s) | 223, 523 |
| HinP1I | GCGC | 2 cut(s) | 223, 523 |
| HincII | GTYRAC | 1 cut(s) | 871 |
| HindII | GTYRAC | 1 cut(s) | 871 |
| HinfI | GANTC | 4 cut(s) | 240, 419, 498, 1258 |
| HpaII | CCGG | 5 cut(s) | 6, 579, 965, 1112, 1503 |
| HphI | GGTGA | 4 cut(s) | 529, 719, 1364, 1400 |
| Hpy166II | GTNNAC | 6 cut(s) | 733, 852, 871, 970, 1178, 1500 |
| Hpy188I | TCNGA | 2 cut(s) | 704, 1509 |
| Hpy188III | TCNNGA | 7 cut(s) | 6, 416, 1013, 1041, 1112, 1156, 1474 |
| Hpy8I | GTNNAC | 6 cut(s) | 733, 852, 871, 970, 1178, 1500 |
| HpyAV | CCTTC | 4 cut(s) | 148, 290, 670, 817 |
| HpyCH4III | ACNGT | 5 cut(s) | 427, 448, 725, 1469, 1532 |
| HpyCH4IV | ACGT | 1 cut(s) | 848 |
| HpyF10VI | GCNNNNNNNGC | 3 cut(s) | 554, 1133, 1145 |
| HpyF3I | CTNAG | 5 cut(s) | 150, 797, 1157, 1422, 1488 |
| HpySE526I | ACGT | 1 cut(s) | 848 |
| Hsp92II | CATG | 7 cut(s) | 26, 86, 1016, 1133, 1148, 1249, 1316 |
| HspAI | GCGC | 2 cut(s) | 223, 523 |
| Kpn2I | TCCGGA | 2 cut(s) | 5, 1111 |
| Ksp22I | TGATCA | 2 cut(s) | 103, 318 |
| Kzo9I | GATC | 6 cut(s) | 103, 159, 183, 318, 745, 880 |
| LguI | GCTCTTC | 1 cut(s) | 1042 |
| LmnI | GCTCC | 3 cut(s) | 961, 1133, 1423 |
| Lsp1109I | GCAGC | 6 cut(s) | 85, 182, 368, 557, 1123, 1135 |
| LweI | GCATC | 7 cut(s) | 157, 205, 381, 510, 706, 976, 1192 |
| MaeI | CTAG | 1 cut(s) | 1041 |
| MaeII | ACGT | 1 cut(s) | 848 |
| MaeIII | GTNAC | 3 cut(s) | 325, 1519, 1532 |
| MalI | GATC | 6 cut(s) | 105, 161, 185, 320, 747, 882 |
| MboI | GATC | 6 cut(s) | 103, 159, 183, 318, 745, 880 |
| MboII | GAAGA | 7 cut(s) | 374, 722, 937, 1029, 1069, 1099, 1522 |
| MlsI | TGGCCA | 1 cut(s) | 280 |
| MluNI | TGGCCA | 1 cut(s) | 280 |
| MlyI | GAGTC | 2 cut(s) | 413, 507 |
| Mox20I | TGGCCA | 1 cut(s) | 280 |
| MroI | TCCGGA | 2 cut(s) | 5, 1111 |
| MroXI | GAANNNNTTC | 2 cut(s) | 18, 1076 |
| MscI | TGGCCA | 1 cut(s) | 280 |
| MseI | TTAA | 3 cut(s) | 177, 806, 1107 |
| MslI | CAYNNNNRTG | 2 cut(s) | 81, 389 |
| Msp20I | TGGCCA | 1 cut(s) | 280 |
| MspA1I | CMGCKG | 3 cut(s) | 359, 548, 1487 |
| MspI | CCGG | 5 cut(s) | 6, 579, 965, 1112, 1503 |
| MspR9I | CCNGG | 2 cut(s) | 41, 335 |
| MvaI | CCWGG | 2 cut(s) | 41, 335 |
| MwoI | GCNNNNNNNGC | 3 cut(s) | 554, 1133, 1145 |
| NdeII | GATC | 6 cut(s) | 103, 159, 183, 318, 745, 880 |
| NlaIII | CATG | 7 cut(s) | 26, 86, 1016, 1133, 1148, 1249, 1316 |
| NlaIV | GGNNCC | 2 cut(s) | 301, 963 |
| NmeAIII | GCCGAG | 1 cut(s) | 1288 |
| NmuCI | GTSAC | 1 cut(s) | 1519 |
| NsbI | TGCGCA | 1 cut(s) | 524 |
| NspI | RCATGY | 1 cut(s) | 86 |
| PagI | TCATGA | 1 cut(s) | 1012 |
| PciI | ACATGT | 1 cut(s) | 82 |
| PciSI | GCTCTTC | 1 cut(s) | 1042 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 53 |
| PdmI | GAANNNNTTC | 2 cut(s) | 18, 1076 |
| PfeI | GAWTC | 2 cut(s) | 240, 1258 |
| PinAI | ACCGGT | 1 cut(s) | 1502 |
| PkrI | GCNGC | 7 cut(s) | 75, 172, 358, 547, 550, 1138, 1150 |
| PleI | GAGTC | 2 cut(s) | 413, 506 |
| PpsI | GAGTC | 2 cut(s) | 413, 506 |
| PscI | ACATGT | 1 cut(s) | 82 |
| PshBI | ATTAAT | 1 cut(s) | 1107 |
| PsiI | TTATAA | 2 cut(s) | 443, 902 |
| Psp6I | CCWGG | 2 cut(s) | 39, 333 |
| PspGI | CCWGG | 2 cut(s) | 39, 333 |
| PspN4I | GGNNCC | 2 cut(s) | 301, 963 |
| PspPI | GGNCC | 1 cut(s) | 117 |
| PstI | CTGCAG | 1 cut(s) | 1298 |
| PvuII | CAGCTG | 1 cut(s) | 359 |
| RsaI | GTAC | 1 cut(s) | 889 |
| RsaNI | GTAC | 1 cut(s) | 888 |
| RseI | CAYNNNNRTG | 2 cut(s) | 81, 389 |
| SapI | GCTCTTC | 1 cut(s) | 1042 |
| SaqAI | TTAA | 3 cut(s) | 177, 806, 1107 |
| SatI | GCNGC | 7 cut(s) | 74, 171, 357, 546, 549, 1137, 1149 |
| Sau3AI | GATC | 6 cut(s) | 103, 159, 183, 318, 745, 880 |
| Sau96I | GGNCC | 1 cut(s) | 117 |
| SchI | GAGTC | 2 cut(s) | 413, 507 |
| ScrFI | CCNGG | 2 cut(s) | 41, 335 |
| SfaNI | GCATC | 7 cut(s) | 157, 205, 381, 510, 706, 976, 1192 |
| SfcI | CTRYAG | 2 cut(s) | 1281, 1294 |
| SinI | GGWCC | 1 cut(s) | 117 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 81, 389 |
| SmlI | CTYRAG | 2 cut(s) | 566, 1474 |
| SmoI | CTYRAG | 2 cut(s) | 566, 1474 |
| SsiI | CCGC | 8 cut(s) | 65, 339, 394, 437, 453, 548, 1121, 1485 |
| SspI | AATATT | 1 cut(s) | 1408 |
| SspMI | CTAG | 1 cut(s) | 1041 |
| StyD4I | CCNGG | 2 cut(s) | 39, 333 |
| StyI | CCWWGG | 2 cut(s) | 789, 1050 |
| TaaI | ACNGT | 5 cut(s) | 427, 448, 725, 1469, 1532 |
| TaiI | ACGT | 1 cut(s) | 851 |
| TaqI | TCGA | 6 cut(s) | 612, 717, 744, 883, 1440, 1458 |
| TauI | GCSGC | 1 cut(s) | 551 |
| TfiI | GAWTC | 2 cut(s) | 240, 1258 |
| Tru1I | TTAA | 3 cut(s) | 177, 806, 1107 |
| Tru9I | TTAA | 3 cut(s) | 177, 806, 1107 |
| TscAI | CASTG | 4 cut(s) | 148, 250, 688, 784 |
| TseFI | GTSAC | 1 cut(s) | 1519 |
| TseI | GCWGC | 6 cut(s) | 73, 170, 356, 545, 1136, 1148 |
| Tsp45I | GTSAC | 1 cut(s) | 1519 |
| TspGWI | ACGGA | 2 cut(s) | 196, 1084 |
| TspRI | CASTG | 4 cut(s) | 148, 250, 688, 784 |
| VpaK11BI | GGWCC | 1 cut(s) | 117 |
| VspI | ATTAAT | 1 cut(s) | 1107 |
| XapI | RAATTY | 3 cut(s) | 920, 1044, 1391 |
| XbaI | TCTAGA | 1 cut(s) | 1040 |
| XceI | RCATGY | 1 cut(s) | 86 |
| XmiI | GTMKAC | 1 cut(s) | 1499 |
| XmnI | GAANNNNTTC | 2 cut(s) | 18, 1076 |
| XspI | CTAG | 1 cut(s) | 1041 |