Belongs to the heat shock protein 70 family
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc16I | TGCGCA | 2 cut(s) | 314, 425 |
| AccIII | TCCGGA | 1 cut(s) | 223 |
| AciI | CCGC | 1 cut(s) | 565 |
| AcoI | YGGCCR | 1 cut(s) | 566 |
| AcuI | CTGAAG | 2 cut(s) | 635, 671 |
| AfaI | GTAC | 1 cut(s) | 536 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 609, 616 |
| AflII | CTTAAG | 1 cut(s) | 89 |
| AflIII | ACRYGT | 1 cut(s) | 253 |
| AgsI | TTSAA | 4 cut(s) | 598, 625, 661, 709 |
| AjnI | CCWGG | 1 cut(s) | 575 |
| AleI | CACNNNNGTG | 1 cut(s) | 590 |
| AloI | GAACNNNNNNTCC | 2 cut(s) | 200, 232 |
| AluBI | AGCT | 1 cut(s) | 104 |
| AluI | AGCT | 1 cut(s) | 104 |
| Alw26I | GTCTC | 1 cut(s) | 73 |
| Aor13HI | TCCGGA | 1 cut(s) | 223 |
| AoxI | GGCC | 4 cut(s) | 9, 387, 566, 600 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 620 |
| AspLEI | GCGC | 2 cut(s) | 315, 426 |
| AspS9I | GGNCC | 2 cut(s) | 579, 722 |
| AsuC2I | CCSGG | 1 cut(s) | 610 |
| AsuHPI | GGTGA | 3 cut(s) | 112, 334, 533 |
| AvaII | GGWCC | 2 cut(s) | 579, 722 |
| BbsI | GAAGAC | 2 cut(s) | 292, 328 |
| BccI | CCATC | 2 cut(s) | 199, 544 |
| BceAI | ACGGC | 1 cut(s) | 575 |
| BciT130I | CCWGG | 1 cut(s) | 577 |
| BclI | TGATCA | 2 cut(s) | 108, 351 |
| BcnI | CCSGG | 1 cut(s) | 610 |
| BcoDI | GTCTC | 1 cut(s) | 73 |
| BfaI | CTAG | 1 cut(s) | 542 |
| BfmI | CTRYAG | 2 cut(s) | 193, 726 |
| BfrI | CTTAAG | 1 cut(s) | 89 |
| BisI | GCNGC | 1 cut(s) | 566 |
| BlsI | GCNGC | 1 cut(s) | 567 |
| Bme1390I | CCNGG | 2 cut(s) | 577, 610 |
| Bme18I | GGWCC | 2 cut(s) | 579, 722 |
| BmgT120I | GGNCC | 2 cut(s) | 579, 722 |
| BmiI | GGNNCC | 2 cut(s) | 218, 723 |
| BmrFI | CCNGG | 2 cut(s) | 577, 610 |
| BmsI | GCATC | 2 cut(s) | 126, 300 |
| BpiI | GAAGAC | 2 cut(s) | 292, 328 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 205, 237 |
| BpuMI | CCSGG | 1 cut(s) | 610 |
| BsaBI | GATNNNNATC | 1 cut(s) | 353 |
| BsaJI | CCNNGG | 3 cut(s) | 39, 384, 517 |
| BsaWI | WCCGGW | 1 cut(s) | 223 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 609, 616 |
| Bse1I | ACTGG | 1 cut(s) | 87 |
| Bse3DI | GCAATG | 1 cut(s) | 72 |
| Bse8I | GATNNNNATC | 1 cut(s) | 353 |
| BseAI | TCCGGA | 1 cut(s) | 223 |
| BseBI | CCWGG | 1 cut(s) | 577 |
| BseDI | CCNNGG | 3 cut(s) | 39, 384, 517 |
| BseGI | GGATG | 1 cut(s) | 271 |
| BseJI | GATNNNNATC | 1 cut(s) | 353 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 609, 616 |
| BseMI | GCAATG | 1 cut(s) | 72 |
| BseMII | CTCAG | 2 cut(s) | 150, 171 |
| BseNI | ACTGG | 1 cut(s) | 87 |
| BseRI | GAGGAG | 1 cut(s) | 20 |
| BseX3I | CGGCCG | 1 cut(s) | 566 |
| Bsh1285I | CGRYCG | 1 cut(s) | 569 |
| BshFI | GGCC | 4 cut(s) | 11, 389, 568, 602 |
| BsiEI | CGRYCG | 1 cut(s) | 569 |
| BsiSI | CCGG | 2 cut(s) | 224, 610 |
| BslFI | GGGAC | 1 cut(s) | 735 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 609, 616 |
| BsmAI | GTCTC | 1 cut(s) | 73 |
| BsmFI | GGGAC | 1 cut(s) | 735 |
| BsmI | GAATGC | 2 cut(s) | 567, 577 |
| BsnI | GGCC | 4 cut(s) | 11, 389, 568, 602 |
| Bsp13I | TCCGGA | 1 cut(s) | 223 |
| Bsp1407I | TGTACA | 1 cut(s) | 534 |
| Bsp143I | GATC | 3 cut(s) | 108, 351, 473 |
| BspACI | CCGC | 1 cut(s) | 565 |
| BspANI | GGCC | 4 cut(s) | 11, 389, 568, 602 |
| BspCNI | CTCAG | 2 cut(s) | 149, 170 |
| BspEI | TCCGGA | 1 cut(s) | 223 |
| BspLI | GGNNCC | 2 cut(s) | 218, 723 |
| BspTI | CTTAAG | 1 cut(s) | 89 |
| BsrDI | GCAATG | 1 cut(s) | 72 |
| BsrGI | TGTACA | 1 cut(s) | 534 |
| BsrI | ACTGG | 1 cut(s) | 87 |
| BssECI | CCNNGG | 3 cut(s) | 39, 384, 517 |
| BssMI | GATC | 3 cut(s) | 108, 351, 473 |
| BssT1I | CCWWGG | 3 cut(s) | 39, 384, 517 |
| Bst2UI | CCWGG | 1 cut(s) | 577 |
| Bst4CI | ACNGT | 2 cut(s) | 467, 739 |
| Bst6I | CTCTTC | 2 cut(s) | 137, 195 |
| BstAFI | CTTAAG | 1 cut(s) | 89 |
| BstAUI | TGTACA | 1 cut(s) | 534 |
| BstC8I | GCNNGC | 1 cut(s) | 428 |
| BstDEI | CTNAG | 2 cut(s) | 136, 157 |
| BstF5I | GGATG | 1 cut(s) | 271 |
| BstHHI | GCGC | 2 cut(s) | 315, 426 |
| BstKTI | GATC | 3 cut(s) | 111, 354, 476 |
| BstMAI | GTCTC | 1 cut(s) | 73 |
| BstMBI | GATC | 3 cut(s) | 108, 351, 473 |
| BstMCI | CGRYCG | 1 cut(s) | 569 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 71, 452 |
| BstNI | CCWGG | 1 cut(s) | 577 |
| BstNSI | RCATGY | 2 cut(s) | 257, 430 |
| BstSCI | CCNGG | 2 cut(s) | 575, 608 |
| BstSFI | CTRYAG | 2 cut(s) | 193, 726 |
| BstV2I | GAAGAC | 2 cut(s) | 292, 328 |
| BstZI | CGGCCG | 1 cut(s) | 566 |
| BsuRI | GGCC | 4 cut(s) | 11, 389, 568, 602 |
| BtsCI | GGATG | 1 cut(s) | 271 |
| BtsI | GCAGTG | 1 cut(s) | 585 |
| BtsIMutI | CAGTG | 2 cut(s) | 472, 585 |
| Cac8I | GCNNGC | 1 cut(s) | 428 |
| CfoI | GCGC | 2 cut(s) | 315, 426 |
| Cfr13I | GGNCC | 2 cut(s) | 579, 722 |
| Csp6I | GTAC | 1 cut(s) | 535 |
| CviAII | CATG | 5 cut(s) | 254, 358, 421, 427, 666 |
| CviJI | RGCY | 9 cut(s) | 11, 53, 74, 104, 389, 568, 602, 613, 692 |
| CviKI_1 | RGCY | 9 cut(s) | 11, 53, 74, 104, 389, 568, 602, 613, 692 |
| CviQI | GTAC | 1 cut(s) | 535 |
| DdeI | CTNAG | 2 cut(s) | 136, 157 |
| DpnI | GATC | 3 cut(s) | 110, 353, 475 |
| DpnII | GATC | 3 cut(s) | 108, 351, 473 |
| EaeI | YGGCCR | 1 cut(s) | 566 |
| EagI | CGGCCG | 1 cut(s) | 566 |
| Eam1104I | CTCTTC | 2 cut(s) | 137, 195 |
| EarI | CTCTTC | 2 cut(s) | 137, 195 |
| EclXI | CGGCCG | 1 cut(s) | 566 |
| Eco130I | CCWWGG | 3 cut(s) | 39, 384, 517 |
| Eco147I | AGGCCT | 1 cut(s) | 602 |
| Eco47I | GGWCC | 2 cut(s) | 579, 722 |
| Eco52I | CGGCCG | 1 cut(s) | 566 |
| Eco57I | CTGAAG | 2 cut(s) | 635, 671 |
| EcoO109I | RGGNCCY | 2 cut(s) | 579, 722 |
| EcoRII | CCWGG | 1 cut(s) | 575 |
| EcoT14I | CCWWGG | 3 cut(s) | 39, 384, 517 |
| ErhI | CCWWGG | 3 cut(s) | 39, 384, 517 |
| FaeI | CATG | 5 cut(s) | 257, 361, 424, 430, 669 |
| FaqI | GGGAC | 1 cut(s) | 735 |
| FatI | CATG | 5 cut(s) | 253, 357, 420, 426, 665 |
| FbaI | TGATCA | 2 cut(s) | 108, 351 |
| Fnu4HI | GCNGC | 1 cut(s) | 566 |
| FokI | GGATG | 1 cut(s) | 278 |
| Fsp4HI | GCNGC | 1 cut(s) | 566 |
| FspAI | RTGCGCAY | 1 cut(s) | 425 |
| FspBI | CTAG | 1 cut(s) | 542 |
| FspI | TGCGCA | 2 cut(s) | 314, 425 |
| GlaI | GCGC | 2 cut(s) | 314, 425 |
| GluI | GCNGC | 1 cut(s) | 566 |
| HaeIII | GGCC | 4 cut(s) | 11, 389, 568, 602 |
| HapII | CCGG | 2 cut(s) | 224, 610 |
| HhaI | GCGC | 2 cut(s) | 315, 426 |
| Hin1II | CATG | 5 cut(s) | 257, 361, 424, 430, 669 |
| Hin6I | GCGC | 2 cut(s) | 313, 424 |
| HinP1I | GCGC | 2 cut(s) | 313, 424 |
| HinfI | GANTC | 3 cut(s) | 164, 209, 441 |
| HpaII | CCGG | 2 cut(s) | 224, 610 |
| HphI | GGTGA | 3 cut(s) | 112, 334, 533 |
| Hpy166II | GTNNAC | 1 cut(s) | 537 |
| Hpy188I | TCNGA | 4 cut(s) | 139, 160, 169, 651 |
| Hpy188III | TCNNGA | 3 cut(s) | 224, 529, 542 |
| Hpy8I | GTNNAC | 1 cut(s) | 537 |
| HpyAV | CCTTC | 5 cut(s) | 610, 628, 646, 682, 703 |
| HpyCH4III | ACNGT | 2 cut(s) | 467, 739 |
| HpyCH4V | TGCA | 2 cut(s) | 292, 500 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 71, 452 |
| HpyF3I | CTNAG | 2 cut(s) | 136, 157 |
| Hsp92II | CATG | 5 cut(s) | 257, 361, 424, 430, 669 |
| HspAI | GCGC | 2 cut(s) | 313, 424 |
| Kpn2I | TCCGGA | 1 cut(s) | 223 |
| Ksp22I | TGATCA | 2 cut(s) | 108, 351 |
| Kzo9I | GATC | 3 cut(s) | 108, 351, 473 |
| LmnI | GCTCC | 2 cut(s) | 101, 451 |
| LpnPI | CCDG | 8 cut(s) | 68, 237, 285, 339, 562, 589, 623, 738 |
| LweI | GCATC | 2 cut(s) | 126, 300 |
| MaeI | CTAG | 1 cut(s) | 542 |
| MalI | GATC | 3 cut(s) | 110, 353, 475 |
| MboI | GATC | 3 cut(s) | 108, 351, 473 |
| MboII | GAAGA | 4 cut(s) | 124, 212, 292, 333 |
| MluCI | AATT | 1 cut(s) | 511 |
| MlyI | GAGTC | 1 cut(s) | 450 |
| MnlI | CCTC | 7 cut(s) | 63, 207, 231, 256, 388, 397, 694 |
| MroI | TCCGGA | 1 cut(s) | 223 |
| MroXI | GAANNNNTTC | 1 cut(s) | 620 |
| MseI | TTAA | 3 cut(s) | 90, 489, 732 |
| MslI | CAYNNNNRTG | 4 cut(s) | 347, 378, 425, 590 |
| MspCI | CTTAAG | 1 cut(s) | 89 |
| MspI | CCGG | 2 cut(s) | 224, 610 |
| MspR9I | CCNGG | 2 cut(s) | 577, 610 |
| Mva1269I | GAATGC | 2 cut(s) | 567, 577 |
| MvaI | CCWGG | 1 cut(s) | 577 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 71, 452 |
| NciI | CCSGG | 1 cut(s) | 610 |
| NdeII | GATC | 3 cut(s) | 108, 351, 473 |
| NlaIII | CATG | 5 cut(s) | 257, 361, 424, 430, 669 |
| NlaIV | GGNNCC | 2 cut(s) | 218, 723 |
| NsbI | TGCGCA | 2 cut(s) | 314, 425 |
| NspI | RCATGY | 2 cut(s) | 257, 430 |
| OliI | CACNNNNGTG | 1 cut(s) | 590 |
| PaeI | GCATGC | 1 cut(s) | 430 |
| PceI | AGGCCT | 1 cut(s) | 602 |
| PciI | ACATGT | 1 cut(s) | 253 |
| PctI | GAATGC | 2 cut(s) | 567, 577 |
| PdmI | GAANNNNTTC | 1 cut(s) | 620 |
| PfeI | GAWTC | 2 cut(s) | 164, 209 |
| PkrI | GCNGC | 1 cut(s) | 567 |
| PleI | GAGTC | 1 cut(s) | 449 |
| PpsI | GAGTC | 1 cut(s) | 449 |
| PpuMI | RGGWCCY | 2 cut(s) | 579, 722 |
| PscI | ACATGT | 1 cut(s) | 253 |
| Psp5II | RGGWCCY | 2 cut(s) | 579, 722 |
| Psp6I | CCWGG | 1 cut(s) | 575 |
| PspGI | CCWGG | 1 cut(s) | 575 |
| PspN4I | GGNNCC | 2 cut(s) | 218, 723 |
| PspPI | GGNCC | 2 cut(s) | 579, 722 |
| PspPPI | RGGWCCY | 2 cut(s) | 579, 722 |
| RsaI | GTAC | 1 cut(s) | 536 |
| RsaNI | GTAC | 1 cut(s) | 535 |
| RseI | CAYNNNNRTG | 4 cut(s) | 347, 378, 425, 590 |
| SaqAI | TTAA | 3 cut(s) | 90, 489, 732 |
| SatI | GCNGC | 1 cut(s) | 566 |
| Sau3AI | GATC | 3 cut(s) | 108, 351, 473 |
| Sau96I | GGNCC | 2 cut(s) | 579, 722 |
| SchI | GAGTC | 1 cut(s) | 450 |
| ScrFI | CCNGG | 2 cut(s) | 577, 610 |
| SetI | ASST | 9 cut(s) | 106, 218, 328, 399, 408, 584, 630, 705, 727 |
| SfaNI | GCATC | 2 cut(s) | 126, 300 |
| SfcI | CTRYAG | 2 cut(s) | 193, 726 |
| SinI | GGWCC | 2 cut(s) | 579, 722 |
| SmiMI | CAYNNNNRTG | 4 cut(s) | 347, 378, 425, 590 |
| SmlI | CTYRAG | 1 cut(s) | 89 |
| SmoI | CTYRAG | 1 cut(s) | 89 |
| SphI | GCATGC | 1 cut(s) | 430 |
| Sse9I | AATT | 1 cut(s) | 511 |
| SseBI | AGGCCT | 1 cut(s) | 602 |
| SsiI | CCGC | 1 cut(s) | 565 |
| SspI | AATATT | 1 cut(s) | 337 |
| SspMI | CTAG | 1 cut(s) | 542 |
| StuI | AGGCCT | 1 cut(s) | 602 |
| StyD4I | CCNGG | 2 cut(s) | 575, 608 |
| StyI | CCWWGG | 3 cut(s) | 39, 384, 517 |
| TaaI | ACNGT | 2 cut(s) | 467, 739 |
| TaqI | TCGA | 3 cut(s) | 212, 261, 472 |
| TasI | AATT | 1 cut(s) | 511 |
| TatI | WGTACW | 1 cut(s) | 534 |
| TauI | GCSGC | 1 cut(s) | 568 |
| TfiI | GAWTC | 2 cut(s) | 164, 209 |
| Tru1I | TTAA | 3 cut(s) | 90, 489, 732 |
| Tru9I | TTAA | 3 cut(s) | 90, 489, 732 |
| TscAI | CASTG | 2 cut(s) | 472, 592 |
| TspDTI | ATGAA | 3 cut(s) | 47, 195, 409 |
| TspRI | CASTG | 2 cut(s) | 472, 592 |
| Vha464I | CTTAAG | 1 cut(s) | 89 |
| VpaK11BI | GGWCC | 2 cut(s) | 579, 722 |
| XbaI | TCTAGA | 1 cut(s) | 541 |
| XceI | RCATGY | 2 cut(s) | 257, 430 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 46 |
| XmnI | GAANNNNTTC | 1 cut(s) | 620 |
| XspI | CTAG | 1 cut(s) | 542 |