Belongs to the heat shock protein 70 family
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AatII | GACGTC | 1 cut(s) | 1008 |
| AccIII | TCCGGA | 1 cut(s) | 1117 |
| AciI | CCGC | 8 cut(s) | 5, 71, 443, 627, 630, 666, 971, 1220 |
| AclWI | GGATC | 1 cut(s) | 47 |
| AcoI | YGGCCR | 1 cut(s) | 1516 |
| AcsI | RAATTY | 3 cut(s) | 926, 1050, 1448 |
| AcuI | CTGAAG | 2 cut(s) | 42, 1521 |
| AcyI | GRCGYC | 1 cut(s) | 1005 |
| AfaI | GTAC | 4 cut(s) | 593, 634, 1062, 1372 |
| AfiI | CCNNNNNNNGG | 3 cut(s) | 693, 1299, 1525 |
| AflII | CTTAAG | 1 cut(s) | 1072 |
| AflIII | ACRYGT | 1 cut(s) | 1506 |
| AhlI | ACTAGT | 1 cut(s) | 1553 |
| AjiI | CACGTC | 1 cut(s) | 781 |
| AjnI | CCWGG | 1 cut(s) | 45 |
| AjuI | GAANNNNNNNTTGG | 4 cut(s) | 150, 182, 968, 1000 |
| AluBI | AGCT | 6 cut(s) | 20, 190, 503, 1099, 1189, 1430 |
| AluI | AGCT | 6 cut(s) | 20, 190, 503, 1099, 1189, 1430 |
| Alw26I | GTCTC | 2 cut(s) | 835, 1232 |
| AlwI | GGATC | 1 cut(s) | 47 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 458 |
| Ama87I | CYCGRG | 2 cut(s) | 918, 1229 |
| Aor13HI | TCCGGA | 1 cut(s) | 1117 |
| AoxI | GGCC | 7 cut(s) | 284, 343, 414, 514, 568, 921, 1516 |
| ApeKI | GCWGC | 8 cut(s) | 176, 362, 500, 551, 554, 1011, 1142, 1154 |
| ApoI | RAATTY | 3 cut(s) | 926, 1050, 1448 |
| AseI | ATTAAT | 2 cut(s) | 233, 1113 |
| Asp700I | GAANNNNTTC | 2 cut(s) | 202, 1188 |
| AspS9I | GGNCC | 3 cut(s) | 305, 343, 921 |
| AsuHPI | GGTGA | 6 cut(s) | 139, 182, 695, 725, 748, 1412 |
| AvaI | CYCGRG | 2 cut(s) | 918, 1229 |
| AvaII | GGWCC | 1 cut(s) | 305 |
| AxyI | CCTNAGG | 1 cut(s) | 1056 |
| BalI | TGGCCA | 1 cut(s) | 1518 |
| BauI | CACGAG | 1 cut(s) | 61 |
| BbsI | GAAGAC | 4 cut(s) | 723, 849, 1075, 1509 |
| BbvCI | CCTCAGC | 1 cut(s) | 411 |
| BbvI | GCAGC | 8 cut(s) | 163, 349, 512, 538, 566, 1023, 1129, 1141 |
| BccI | CCATC | 2 cut(s) | 139, 221 |
| BceAI | ACGGC | 1 cut(s) | 1337 |
| BciT130I | CCWGG | 1 cut(s) | 47 |
| BciVI | GTATCC | 1 cut(s) | 691 |
| BclI | TGATCA | 2 cut(s) | 85, 1164 |
| BcoDI | GTCTC | 2 cut(s) | 835, 1232 |
| BcuI | ACTAGT | 1 cut(s) | 1553 |
| BfaI | CTAG | 3 cut(s) | 650, 722, 1554 |
| BfmI | CTRYAG | 3 cut(s) | 363, 552, 1155 |
| BfrI | CTTAAG | 1 cut(s) | 1072 |
| BfuI | GTATCC | 1 cut(s) | 691 |
| Bme1390I | CCNGG | 1 cut(s) | 47 |
| Bme18I | GGWCC | 1 cut(s) | 305 |
| BmeT110I | CYCGRG | 2 cut(s) | 918, 1229 |
| BmgBI | CACGTC | 1 cut(s) | 781 |
| BmgT120I | GGNCC | 3 cut(s) | 305, 343, 921 |
| BmiI | GGNNCC | 1 cut(s) | 969 |
| BmrFI | CCNGG | 1 cut(s) | 47 |
| BmrI | ACTGGG | 1 cut(s) | 1251 |
| BmsI | GCATC | 5 cut(s) | 142, 163, 211, 387, 1023 |
| BmuI | ACTGGG | 1 cut(s) | 1251 |
| BpiI | GAAGAC | 4 cut(s) | 723, 849, 1075, 1509 |
| BpmI | CTGGAG | 1 cut(s) | 1410 |
| Bpu10I | CCTNAGC | 1 cut(s) | 411 |
| BsaHI | GRCGYC | 1 cut(s) | 1005 |
| BsaJI | CCNNGG | 4 cut(s) | 46, 421, 706, 1433 |
| BsaWI | WCCGGW | 1 cut(s) | 1117 |
| Bsc4I | CCNNNNNNNGG | 3 cut(s) | 693, 1299, 1525 |
| Bse118I | RCCGGY | 1 cut(s) | 512 |
| Bse1I | ACTGG | 4 cut(s) | 168, 790, 1246, 1519 |
| Bse21I | CCTNAGG | 1 cut(s) | 1056 |
| Bse3DI | GCAATG | 1 cut(s) | 558 |
| BseAI | TCCGGA | 1 cut(s) | 1117 |
| BseBI | CCWGG | 1 cut(s) | 47 |
| BseDI | CCNNGG | 4 cut(s) | 46, 421, 706, 1433 |
| BseGI | GGATG | 3 cut(s) | 1014, 1126, 1216 |
| BseLI | CCNNNNNNNGG | 3 cut(s) | 693, 1299, 1525 |
| BseMI | GCAATG | 1 cut(s) | 558 |
| BseMII | CTCAG | 1 cut(s) | 402 |
| BseNI | ACTGG | 4 cut(s) | 168, 790, 1246, 1519 |
| BseRI | GAGGAG | 1 cut(s) | 853 |
| BseXI | GCAGC | 8 cut(s) | 163, 349, 512, 538, 566, 1023, 1129, 1141 |
| BseYI | CCCAGC | 1 cut(s) | 692 |
| Bsh1285I | CGRYCG | 1 cut(s) | 447 |
| BshFI | GGCC | 7 cut(s) | 286, 345, 416, 516, 570, 923, 1518 |
| BsiEI | CGRYCG | 1 cut(s) | 447 |
| BsiHKCI | CYCGRG | 2 cut(s) | 918, 1229 |
| BsiSI | CCGG | 2 cut(s) | 513, 1118 |
| BslFI | GGGAC | 3 cut(s) | 64, 440, 1268 |
| BslI | CCNNNNNNNGG | 3 cut(s) | 693, 1299, 1525 |
| BsmAI | GTCTC | 2 cut(s) | 835, 1232 |
| BsmFI | GGGAC | 3 cut(s) | 64, 440, 1268 |
| BsmI | GAATGC | 1 cut(s) | 143 |
| BsnI | GGCC | 7 cut(s) | 286, 345, 416, 516, 570, 923, 1518 |
| BsoBI | CYCGRG | 2 cut(s) | 918, 1229 |
| Bsp13I | TCCGGA | 1 cut(s) | 1117 |
| Bsp143I | GATC | 4 cut(s) | 39, 85, 109, 1164 |
| BspACI | CCGC | 8 cut(s) | 5, 71, 443, 627, 630, 666, 971, 1220 |
| BspANI | GGCC | 7 cut(s) | 286, 345, 416, 516, 570, 923, 1518 |
| BspCNI | CTCAG | 1 cut(s) | 403 |
| BspEI | TCCGGA | 1 cut(s) | 1117 |
| BspHI | TCATGA | 1 cut(s) | 310 |
| BspLI | GGNNCC | 1 cut(s) | 969 |
| BspMAI | CTGCAG | 3 cut(s) | 367, 556, 1159 |
| BspPI | GGATC | 1 cut(s) | 47 |
| BspQI | GCTCTTC | 1 cut(s) | 1048 |
| BspTI | CTTAAG | 1 cut(s) | 1072 |
| BsrDI | GCAATG | 1 cut(s) | 558 |
| BsrFI | RCCGGY | 1 cut(s) | 512 |
| BsrI | ACTGG | 4 cut(s) | 168, 790, 1246, 1519 |
| BssAI | RCCGGY | 1 cut(s) | 512 |
| BssECI | CCNNGG | 4 cut(s) | 46, 421, 706, 1433 |
| BssMI | GATC | 4 cut(s) | 39, 85, 109, 1164 |
| BssNI | GRCGYC | 1 cut(s) | 1005 |
| BssSI | CACGAG | 1 cut(s) | 61 |
| BssT1I | CCWWGG | 2 cut(s) | 421, 706 |
| Bst2BI | CACGAG | 1 cut(s) | 61 |
| Bst2UI | CCWGG | 1 cut(s) | 47 |
| Bst4CI | ACNGT | 8 cut(s) | 433, 454, 746, 881, 1139, 1264, 1312, 1381 |
| Bst6I | CTCTTC | 1 cut(s) | 1048 |
| BstACI | GRCGYC | 1 cut(s) | 1005 |
| BstAFI | CTTAAG | 1 cut(s) | 1072 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 560 |
| BstC8I | GCNNGC | 2 cut(s) | 514, 586 |
| BstDEI | CTNAG | 4 cut(s) | 411, 775, 803, 1056 |
| BstF5I | GGATG | 3 cut(s) | 1014, 1126, 1216 |
| BstKTI | GATC | 4 cut(s) | 42, 88, 112, 1167 |
| BstMAI | GTCTC | 2 cut(s) | 835, 1232 |
| BstMBI | GATC | 4 cut(s) | 39, 85, 109, 1164 |
| BstMCI | CGRYCG | 1 cut(s) | 447 |
| BstMWI | GCNNNNNNNGC | 5 cut(s) | 509, 560, 594, 1139, 1151 |
| BstNI | CCWGG | 1 cut(s) | 47 |
| BstNSI | RCATGY | 2 cut(s) | 261, 1510 |
| BstSCI | CCNGG | 1 cut(s) | 45 |
| BstSFI | CTRYAG | 3 cut(s) | 363, 552, 1155 |
| BstV1I | GCAGC | 8 cut(s) | 163, 349, 512, 538, 566, 1023, 1129, 1141 |
| BstV2I | GAAGAC | 4 cut(s) | 723, 849, 1075, 1509 |
| Bsu36I | CCTNAGG | 1 cut(s) | 1056 |
| BsuI | GTATCC | 1 cut(s) | 691 |
| BsuRI | GGCC | 7 cut(s) | 286, 345, 416, 516, 570, 923, 1518 |
| BtrI | CACGTC | 1 cut(s) | 781 |
| BtsCI | GGATG | 3 cut(s) | 1014, 1126, 1216 |
| BtsI | GCAGTG | 2 cut(s) | 687, 1134 |
| BtsIMutI | CAGTG | 5 cut(s) | 475, 514, 687, 1134, 1386 |
| Cac8I | GCNNGC | 2 cut(s) | 514, 586 |
| CaiI | CAGNNNCTG | 1 cut(s) | 458 |
| CciI | TCATGA | 1 cut(s) | 310 |
| Cfr10I | RCCGGY | 1 cut(s) | 512 |
| Cfr13I | GGNCC | 3 cut(s) | 305, 343, 921 |
| CseI | GACGC | 2 cut(s) | 263, 470 |
| Csp6I | GTAC | 4 cut(s) | 592, 633, 1061, 1371 |
| CviAII | CATG | 8 cut(s) | 29, 89, 258, 311, 887, 1258, 1325, 1507 |
| CviQI | GTAC | 4 cut(s) | 592, 633, 1061, 1371 |
| DdeI | CTNAG | 4 cut(s) | 411, 775, 803, 1056 |
| DpnI | GATC | 4 cut(s) | 41, 87, 111, 1166 |
| DpnII | GATC | 4 cut(s) | 39, 85, 109, 1164 |
| EaeI | YGGCCR | 1 cut(s) | 1516 |
| Eam1104I | CTCTTC | 1 cut(s) | 1048 |
| EarI | CTCTTC | 1 cut(s) | 1048 |
| Eco130I | CCWWGG | 2 cut(s) | 421, 706 |
| Eco147I | AGGCCT | 1 cut(s) | 416 |
| Eco47I | GGWCC | 1 cut(s) | 305 |
| Eco57I | CTGAAG | 2 cut(s) | 42, 1521 |
| Eco81I | CCTNAGG | 1 cut(s) | 1056 |
| Eco88I | CYCGRG | 2 cut(s) | 918, 1229 |
| EcoO109I | RGGNCCY | 1 cut(s) | 305 |
| EcoRI | GAATTC | 1 cut(s) | 1050 |
| EcoRII | CCWGG | 1 cut(s) | 45 |
| EcoT14I | CCWWGG | 2 cut(s) | 421, 706 |
| ErhI | CCWWGG | 2 cut(s) | 421, 706 |
| FaeI | CATG | 8 cut(s) | 32, 92, 261, 314, 890, 1261, 1328, 1510 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 758, 790 |
| FaqI | GGGAC | 3 cut(s) | 64, 440, 1268 |
| FatI | CATG | 8 cut(s) | 28, 88, 257, 310, 886, 1257, 1324, 1506 |
| FbaI | TGATCA | 2 cut(s) | 85, 1164 |
| FokI | GGATG | 3 cut(s) | 1001, 1133, 1223 |
| FspBI | CTAG | 3 cut(s) | 650, 722, 1554 |
| GsaI | CCCAGC | 1 cut(s) | 696 |
| GsuI | CTGGAG | 1 cut(s) | 1410 |
| HaeIII | GGCC | 7 cut(s) | 286, 345, 416, 516, 570, 923, 1518 |
| HapII | CCGG | 2 cut(s) | 513, 1118 |
| HgaI | GACGC | 2 cut(s) | 263, 470 |
| Hin1I | GRCGYC | 1 cut(s) | 1005 |
| Hin1II | CATG | 8 cut(s) | 32, 92, 261, 314, 890, 1261, 1328, 1510 |
| HincII | GTYRAC | 1 cut(s) | 1512 |
| HindII | GTYRAC | 1 cut(s) | 1512 |
| HindIII | AAGCTT | 1 cut(s) | 1187 |
| HinfI | GANTC | 4 cut(s) | 473, 992, 1472, 1483 |
| HpaII | CCGG | 2 cut(s) | 513, 1118 |
| HphI | GGTGA | 6 cut(s) | 139, 182, 695, 725, 748, 1412 |
| Hpy166II | GTNNAC | 3 cut(s) | 106, 1250, 1512 |
| Hpy188I | TCNGA | 3 cut(s) | 197, 1434, 1488 |
| Hpy188III | TCNNGA | 9 cut(s) | 37, 266, 311, 760, 1047, 1118, 1162, 1193, 1297 |
| Hpy8I | GTNNAC | 3 cut(s) | 106, 1250, 1512 |
| HpyCH4III | ACNGT | 8 cut(s) | 433, 454, 746, 881, 1139, 1264, 1312, 1381 |
| HpyCH4IV | ACGT | 4 cut(s) | 138, 780, 854, 1005 |
| HpyF10VI | GCNNNNNNNGC | 5 cut(s) | 509, 560, 594, 1139, 1151 |
| HpyF3I | CTNAG | 4 cut(s) | 411, 775, 803, 1056 |
| HpySE526I | ACGT | 4 cut(s) | 138, 780, 854, 1005 |
| Hsp92I | GRCGYC | 1 cut(s) | 1005 |
| Hsp92II | CATG | 8 cut(s) | 32, 92, 261, 314, 890, 1261, 1328, 1510 |
| Kpn2I | TCCGGA | 1 cut(s) | 1117 |
| KroI | GCCGGC | 1 cut(s) | 512 |
| KroNI | GCCGGC | 1 cut(s) | 514 |
| Ksp22I | TGATCA | 2 cut(s) | 85, 1164 |
| Kzo9I | GATC | 4 cut(s) | 39, 85, 109, 1164 |
| LguI | GCTCTTC | 1 cut(s) | 1048 |
| LmnI | GCTCC | 4 cut(s) | 751, 967, 1096, 1435 |
| Lsp1109I | GCAGC | 8 cut(s) | 163, 349, 512, 538, 566, 1023, 1129, 1141 |
| LweI | GCATC | 5 cut(s) | 142, 163, 211, 387, 1023 |
| MaeI | CTAG | 3 cut(s) | 650, 722, 1554 |
| MaeII | ACGT | 4 cut(s) | 138, 780, 854, 1005 |
| MaeIII | GTNAC | 9 cut(s) | 116, 251, 331, 448, 470, 652, 781, 1213, 1312 |
| MalI | GATC | 4 cut(s) | 41, 87, 111, 1166 |
| MboI | GATC | 4 cut(s) | 39, 85, 109, 1164 |
| MboII | GAAGA | 9 cut(s) | 255, 380, 728, 849, 944, 1035, 1075, 1514, 1534 |
| MlsI | TGGCCA | 1 cut(s) | 1518 |
| MluNI | TGGCCA | 1 cut(s) | 1518 |
| MlyI | GAGTC | 1 cut(s) | 467 |
| MmeI | TCCRAC | 2 cut(s) | 231, 1188 |
| Mox20I | TGGCCA | 1 cut(s) | 1518 |
| MroI | TCCGGA | 1 cut(s) | 1117 |
| MroNI | GCCGGC | 1 cut(s) | 512 |
| MroXI | GAANNNNTTC | 2 cut(s) | 202, 1188 |
| MscI | TGGCCA | 1 cut(s) | 1518 |
| MseI | TTAA | 7 cut(s) | 183, 233, 276, 435, 812, 1073, 1113 |
| MslI | CAYNNNNRTG | 1 cut(s) | 1286 |
| Msp20I | TGGCCA | 1 cut(s) | 1518 |
| MspA1I | CMGCKG | 1 cut(s) | 973 |
| MspCI | CTTAAG | 1 cut(s) | 1072 |
| MspI | CCGG | 2 cut(s) | 513, 1118 |
| MspR9I | CCNGG | 1 cut(s) | 47 |
| Mva1269I | GAATGC | 1 cut(s) | 143 |
| MvaI | CCWGG | 1 cut(s) | 47 |
| MwoI | GCNNNNNNNGC | 5 cut(s) | 509, 560, 594, 1139, 1151 |
| NaeI | GCCGGC | 1 cut(s) | 514 |
| NdeII | GATC | 4 cut(s) | 39, 85, 109, 1164 |
| NgoMIV | GCCGGC | 1 cut(s) | 512 |
| NlaIII | CATG | 8 cut(s) | 32, 92, 261, 314, 890, 1261, 1328, 1510 |
| NlaIV | GGNNCC | 1 cut(s) | 969 |
| NmeAIII | GCCGAG | 1 cut(s) | 1300 |
| NmuCI | GTSAC | 6 cut(s) | 251, 331, 448, 470, 781, 1213 |
| NspI | RCATGY | 2 cut(s) | 261, 1510 |
| PagI | TCATGA | 1 cut(s) | 310 |
| PceI | AGGCCT | 1 cut(s) | 416 |
| PciI | ACATGT | 1 cut(s) | 1506 |
| PciSI | GCTCTTC | 1 cut(s) | 1048 |
| PctI | GAATGC | 1 cut(s) | 143 |
| PdiI | GCCGGC | 1 cut(s) | 514 |
| PdmI | GAANNNNTTC | 2 cut(s) | 202, 1188 |
| PfeI | GAWTC | 3 cut(s) | 992, 1472, 1483 |
| PleI | GAGTC | 1 cut(s) | 467 |
| PpsI | GAGTC | 1 cut(s) | 467 |
| PpuMI | RGGWCCY | 1 cut(s) | 305 |
| PscI | ACATGT | 1 cut(s) | 1506 |
| PshBI | ATTAAT | 2 cut(s) | 233, 1113 |
| Psp5II | RGGWCCY | 1 cut(s) | 305 |
| Psp6I | CCWGG | 1 cut(s) | 45 |
| PspFI | CCCAGC | 1 cut(s) | 692 |
| PspGI | CCWGG | 1 cut(s) | 45 |
| PspN4I | GGNNCC | 1 cut(s) | 969 |
| PspPI | GGNCC | 3 cut(s) | 305, 343, 921 |
| PspPPI | RGGWCCY | 1 cut(s) | 305 |
| PstI | CTGCAG | 3 cut(s) | 367, 556, 1159 |
| PstNI | CAGNNNCTG | 1 cut(s) | 458 |
| RsaI | GTAC | 4 cut(s) | 593, 634, 1062, 1372 |
| RsaNI | GTAC | 4 cut(s) | 592, 633, 1061, 1371 |
| RseI | CAYNNNNRTG | 1 cut(s) | 1286 |
| SapI | GCTCTTC | 1 cut(s) | 1048 |
| SaqAI | TTAA | 7 cut(s) | 183, 233, 276, 435, 812, 1073, 1113 |
| Sau3AI | GATC | 4 cut(s) | 39, 85, 109, 1164 |
| Sau96I | GGNCC | 3 cut(s) | 305, 343, 921 |
| SchI | GAGTC | 1 cut(s) | 467 |
| ScrFI | CCNGG | 1 cut(s) | 47 |
| SfaNI | GCATC | 5 cut(s) | 142, 163, 211, 387, 1023 |
| SfcI | CTRYAG | 3 cut(s) | 363, 552, 1155 |
| SinI | GGWCC | 1 cut(s) | 305 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 1286 |
| SmlI | CTYRAG | 1 cut(s) | 1072 |
| SmoI | CTYRAG | 1 cut(s) | 1072 |
| SpeI | ACTAGT | 1 cut(s) | 1553 |
| SseBI | AGGCCT | 1 cut(s) | 416 |
| SsiI | CCGC | 8 cut(s) | 5, 71, 443, 627, 630, 666, 971, 1220 |
| SspMI | CTAG | 3 cut(s) | 650, 722, 1554 |
| StuI | AGGCCT | 1 cut(s) | 416 |
| StyD4I | CCNGG | 1 cut(s) | 45 |
| StyI | CCWWGG | 2 cut(s) | 421, 706 |
| TaaI | ACNGT | 8 cut(s) | 433, 454, 746, 881, 1139, 1264, 1312, 1381 |
| TaiI | ACGT | 4 cut(s) | 141, 783, 857, 1008 |
| TaqI | TCGA | 1 cut(s) | 42 |
| TatI | WGTACW | 1 cut(s) | 1370 |
| TauI | GCSGC | 3 cut(s) | 630, 669, 973 |
| TfiI | GAWTC | 3 cut(s) | 992, 1472, 1483 |
| Tru1I | TTAA | 7 cut(s) | 183, 233, 276, 435, 812, 1073, 1113 |
| Tru9I | TTAA | 7 cut(s) | 183, 233, 276, 435, 812, 1073, 1113 |
| TscAI | CASTG | 5 cut(s) | 475, 514, 694, 1134, 1386 |
| TseFI | GTSAC | 6 cut(s) | 251, 331, 448, 470, 781, 1213 |
| TseI | GCWGC | 8 cut(s) | 176, 362, 500, 551, 554, 1011, 1142, 1154 |
| Tsp45I | GTSAC | 6 cut(s) | 251, 331, 448, 470, 781, 1213 |
| TspDTI | ATGAA | 4 cut(s) | 555, 837, 1197, 1374 |
| TspRI | CASTG | 5 cut(s) | 475, 514, 694, 1134, 1386 |
| Vha464I | CTTAAG | 1 cut(s) | 1072 |
| VpaK11BI | GGWCC | 1 cut(s) | 305 |
| VspI | ATTAAT | 2 cut(s) | 233, 1113 |
| XapI | RAATTY | 3 cut(s) | 926, 1050, 1448 |
| XceI | RCATGY | 2 cut(s) | 261, 1510 |
| XmnI | GAANNNNTTC | 2 cut(s) | 202, 1188 |
| XspI | CTAG | 3 cut(s) | 650, 722, 1554 |
| ZraI | GACGTC | 1 cut(s) | 1006 |