Heat shock cognate 70 kDa
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AasI | GACNNNNNNGTC | 1 cut(s) | 127 |
| AciI | CCGC | 2 cut(s) | 226, 397 |
| AclI | AACGTT | 1 cut(s) | 1354 |
| AclWI | GGATC | 2 cut(s) | 417, 430 |
| AcoI | YGGCCR | 1 cut(s) | 281 |
| AcsI | RAATTY | 5 cut(s) | 242, 752, 923, 1047, 1336 |
| AcuI | CTGAAG | 2 cut(s) | 384, 429 |
| AdeI | CACNNNGTG | 1 cut(s) | 479 |
| AfaI | GTAC | 4 cut(s) | 50, 606, 631, 1240 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 1543 |
| AflIII | ACRYGT | 1 cut(s) | 1524 |
| AhdI | GACNNNNNGTC | 1 cut(s) | 577 |
| AjnI | CCWGG | 1 cut(s) | 567 |
| AjuI | GAANNNNNNNTTGG | 6 cut(s) | 402, 434, 755, 787, 1344, 1376 |
| AluBI | AGCT | 4 cut(s) | 157, 277, 497, 1151 |
| AluI | AGCT | 4 cut(s) | 157, 277, 497, 1151 |
| Alw26I | GTCTC | 2 cut(s) | 832, 1516 |
| AlwI | GGATC | 2 cut(s) | 417, 430 |
| AoxI | GGCC | 4 cut(s) | 281, 511, 565, 1534 |
| ApeKI | GCWGC | 6 cut(s) | 173, 359, 548, 551, 1139, 1151 |
| ApoI | RAATTY | 5 cut(s) | 242, 752, 923, 1047, 1336 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 1079 |
| AspS9I | GGNCC | 2 cut(s) | 120, 302 |
| AsuHPI | GGTGA | 4 cut(s) | 671, 722, 1204, 1394 |
| AvaII | GGWCC | 2 cut(s) | 120, 302 |
| BalI | TGGCCA | 1 cut(s) | 283 |
| BamHI | GGATCC | 1 cut(s) | 422 |
| BauI | CACGAG | 1 cut(s) | 915 |
| BbvCI | CCTCAGC | 1 cut(s) | 1518 |
| BbvI | GCAGC | 6 cut(s) | 185, 346, 560, 563, 1138, 1151 |
| BccI | CCATC | 4 cut(s) | 218, 656, 1319, 1562 |
| BceAI | ACGGC | 1 cut(s) | 1127 |
| BciT130I | CCWGG | 1 cut(s) | 569 |
| BclI | TGATCA | 1 cut(s) | 444 |
| BcoDI | GTCTC | 2 cut(s) | 832, 1516 |
| BfaI | CTAG | 2 cut(s) | 1044, 1415 |
| BfmI | CTRYAG | 3 cut(s) | 507, 1070, 1152 |
| BglII | AGATCT | 1 cut(s) | 369 |
| BisI | GCNGC | 6 cut(s) | 174, 360, 549, 552, 1140, 1152 |
| BlsI | GCNGC | 6 cut(s) | 175, 361, 550, 553, 1141, 1153 |
| BmcAI | AGTACT | 1 cut(s) | 50 |
| Bme1390I | CCNGG | 1 cut(s) | 569 |
| Bme18I | GGWCC | 2 cut(s) | 120, 302 |
| BmeRI | GACNNNNNGTC | 1 cut(s) | 577 |
| BmgT120I | GGNCC | 2 cut(s) | 120, 302 |
| BmiI | GGNNCC | 3 cut(s) | 424, 453, 966 |
| BmrFI | CCNGG | 1 cut(s) | 569 |
| BmsI | GCATC | 7 cut(s) | 208, 241, 263, 513, 892, 979, 1480 |
| BpmI | CTGGAG | 2 cut(s) | 711, 1428 |
| Bpu10I | CCTNAGC | 1 cut(s) | 1518 |
| BpuEI | CTTGAG | 2 cut(s) | 358, 1525 |
| BsaBI | GATNNNNATC | 3 cut(s) | 699, 900, 1553 |
| BsaJI | CCNNGG | 2 cut(s) | 1053, 1177 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 1543 |
| Bse1I | ACTGG | 1 cut(s) | 1445 |
| Bse3DI | GCAATG | 1 cut(s) | 555 |
| Bse8I | GATNNNNATC | 3 cut(s) | 699, 900, 1553 |
| BseBI | CCWGG | 1 cut(s) | 569 |
| BseDI | CCNNGG | 2 cut(s) | 1053, 1177 |
| BseGI | GGATG | 4 cut(s) | 643, 781, 994, 1213 |
| BseJI | GATNNNNATC | 3 cut(s) | 699, 900, 1553 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 1543 |
| BseMI | GCAATG | 1 cut(s) | 555 |
| BseMII | CTCAG | 2 cut(s) | 144, 1509 |
| BseNI | ACTGG | 1 cut(s) | 1445 |
| BseRI | GAGGAG | 1 cut(s) | 850 |
| BseXI | GCAGC | 6 cut(s) | 185, 346, 560, 563, 1138, 1151 |
| BsgI | GTGCAG | 1 cut(s) | 1158 |
| BshFI | GGCC | 4 cut(s) | 283, 513, 567, 1536 |
| BslFI | GGGAC | 1 cut(s) | 1433 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 1543 |
| BsmAI | GTCTC | 2 cut(s) | 832, 1516 |
| BsmFI | GGGAC | 1 cut(s) | 1433 |
| BsmI | GAATGC | 3 cut(s) | 442, 1305, 1597 |
| BsnI | GGCC | 4 cut(s) | 283, 513, 567, 1536 |
| Bsp143I | GATC | 6 cut(s) | 34, 106, 369, 422, 444, 1326 |
| BspACI | CCGC | 2 cut(s) | 226, 397 |
| BspANI | GGCC | 4 cut(s) | 283, 513, 567, 1536 |
| BspCNI | CTCAG | 2 cut(s) | 145, 1510 |
| BspLI | GGNNCC | 3 cut(s) | 424, 453, 966 |
| BspMAI | CTGCAG | 2 cut(s) | 511, 1156 |
| BspPI | GGATC | 2 cut(s) | 417, 430 |
| BspQI | GCTCTTC | 1 cut(s) | 1045 |
| BsrDI | GCAATG | 1 cut(s) | 555 |
| BsrI | ACTGG | 1 cut(s) | 1445 |
| BssECI | CCNNGG | 2 cut(s) | 1053, 1177 |
| BssMI | GATC | 6 cut(s) | 34, 106, 369, 422, 444, 1326 |
| BssSI | CACGAG | 1 cut(s) | 915 |
| BssT1I | CCWWGG | 1 cut(s) | 1053 |
| Bst2BI | CACGAG | 1 cut(s) | 915 |
| Bst2UI | CCWGG | 1 cut(s) | 569 |
| Bst4CI | ACNGT | 5 cut(s) | 354, 430, 451, 743, 1562 |
| Bst6I | CTCTTC | 2 cut(s) | 360, 1045 |
| BstAPI | GCANNNNNTGC | 3 cut(s) | 557, 1066, 1136 |
| BstC8I | GCNNGC | 1 cut(s) | 511 |
| BstDEI | CTNAG | 3 cut(s) | 153, 800, 1518 |
| BstDSI | CCRYGG | 1 cut(s) | 1177 |
| BstF5I | GGATG | 4 cut(s) | 643, 781, 994, 1213 |
| BstKTI | GATC | 6 cut(s) | 37, 109, 372, 425, 447, 1329 |
| BstMAI | GTCTC | 2 cut(s) | 832, 1516 |
| BstMBI | GATC | 6 cut(s) | 34, 106, 369, 422, 444, 1326 |
| BstMWI | GCNNNNNNNGC | 4 cut(s) | 557, 1066, 1136, 1148 |
| BstNI | CCWGG | 1 cut(s) | 569 |
| BstNSI | RCATGY | 2 cut(s) | 822, 1528 |
| BstSCI | CCNGG | 1 cut(s) | 567 |
| BstSFI | CTRYAG | 3 cut(s) | 507, 1070, 1152 |
| BstV1I | GCAGC | 6 cut(s) | 185, 346, 560, 563, 1138, 1151 |
| BstX2I | RGATCY | 2 cut(s) | 369, 422 |
| BstYI | RGATCY | 2 cut(s) | 369, 422 |
| BsuRI | GGCC | 4 cut(s) | 283, 513, 567, 1536 |
| BtgI | CCRYGG | 1 cut(s) | 1177 |
| BtsCI | GGATG | 4 cut(s) | 643, 781, 994, 1213 |
| BtsI | GCAGTG | 1 cut(s) | 684 |
| BtsIMutI | CAGTG | 7 cut(s) | 144, 253, 359, 426, 684, 787, 1567 |
| Cac8I | GCNNGC | 1 cut(s) | 511 |
| Cfr13I | GGNCC | 2 cut(s) | 120, 302 |
| Csp6I | GTAC | 4 cut(s) | 49, 605, 630, 1239 |
| CviAII | CATG | 8 cut(s) | 20, 54, 74, 132, 335, 819, 1016, 1525 |
| CviQI | GTAC | 4 cut(s) | 49, 605, 630, 1239 |
| DdeI | CTNAG | 3 cut(s) | 153, 800, 1518 |
| DpnI | GATC | 6 cut(s) | 36, 108, 371, 424, 446, 1328 |
| DpnII | GATC | 6 cut(s) | 34, 106, 369, 422, 444, 1326 |
| DraIII | CACNNNGTG | 1 cut(s) | 479 |
| DrdI | GACNNNNNNGTC | 1 cut(s) | 127 |
| DriI | GACNNNNNGTC | 1 cut(s) | 577 |
| DseDI | GACNNNNNNGTC | 1 cut(s) | 127 |
| EaeI | YGGCCR | 1 cut(s) | 281 |
| Eam1104I | CTCTTC | 2 cut(s) | 360, 1045 |
| Eam1105I | GACNNNNNGTC | 1 cut(s) | 577 |
| EarI | CTCTTC | 2 cut(s) | 360, 1045 |
| Eco130I | CCWWGG | 1 cut(s) | 1053 |
| Eco147I | AGGCCT | 1 cut(s) | 513 |
| Eco32I | GATATC | 1 cut(s) | 94 |
| Eco47I | GGWCC | 2 cut(s) | 120, 302 |
| Eco57I | CTGAAG | 2 cut(s) | 384, 429 |
| EcoO109I | RGGNCCY | 1 cut(s) | 302 |
| EcoRI | GAATTC | 1 cut(s) | 1047 |
| EcoRII | CCWGG | 1 cut(s) | 567 |
| EcoRV | GATATC | 1 cut(s) | 94 |
| EcoT14I | CCWWGG | 1 cut(s) | 1053 |
| EcoT22I | ATGCAT | 3 cut(s) | 256, 336, 820 |
| ErhI | CCWWGG | 1 cut(s) | 1053 |
| FaeI | CATG | 8 cut(s) | 23, 57, 77, 135, 338, 822, 1019, 1528 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 402, 434 |
| FaqI | GGGAC | 1 cut(s) | 1433 |
| FatI | CATG | 8 cut(s) | 19, 53, 73, 131, 334, 818, 1015, 1524 |
| FauI | CCCGC | 1 cut(s) | 390 |
| FauNDI | CATATG | 1 cut(s) | 1132 |
| FbaI | TGATCA | 1 cut(s) | 444 |
| Fnu4HI | GCNGC | 6 cut(s) | 174, 360, 549, 552, 1140, 1152 |
| FokI | GGATG | 4 cut(s) | 650, 788, 1001, 1220 |
| Fsp4HI | GCNGC | 6 cut(s) | 174, 360, 549, 552, 1140, 1152 |
| FspBI | CTAG | 2 cut(s) | 1044, 1415 |
| GluI | GCNGC | 6 cut(s) | 174, 360, 549, 552, 1140, 1152 |
| GsuI | CTGGAG | 2 cut(s) | 711, 1428 |
| HaeIII | GGCC | 4 cut(s) | 283, 513, 567, 1536 |
| Hin1II | CATG | 8 cut(s) | 23, 57, 77, 135, 338, 822, 1019, 1528 |
| HincII | GTYRAC | 1 cut(s) | 1345 |
| HindII | GTYRAC | 1 cut(s) | 1345 |
| HinfI | GANTC | 1 cut(s) | 1288 |
| HphI | GGTGA | 4 cut(s) | 671, 722, 1204, 1394 |
| Hpy166II | GTNNAC | 4 cut(s) | 103, 1239, 1345, 1530 |
| Hpy188I | TCNGA | 2 cut(s) | 794, 906 |
| Hpy188III | TCNNGA | 8 cut(s) | 305, 1044, 1115, 1159, 1190, 1267, 1315, 1504 |
| Hpy8I | GTNNAC | 4 cut(s) | 103, 1239, 1345, 1530 |
| HpyAV | CCTTC | 8 cut(s) | 8, 151, 293, 332, 1084, 1375, 1537, 1574 |
| HpyCH4III | ACNGT | 5 cut(s) | 354, 430, 451, 743, 1562 |
| HpyCH4IV | ACGT | 2 cut(s) | 1002, 1354 |
| HpyF10VI | GCNNNNNNNGC | 4 cut(s) | 557, 1066, 1136, 1148 |
| HpyF3I | CTNAG | 3 cut(s) | 153, 800, 1518 |
| HpySE526I | ACGT | 2 cut(s) | 1002, 1354 |
| Hsp92II | CATG | 8 cut(s) | 23, 57, 77, 135, 338, 822, 1019, 1528 |
| Ksp22I | TGATCA | 1 cut(s) | 444 |
| Kzo9I | GATC | 6 cut(s) | 34, 106, 369, 422, 444, 1326 |
| LguI | GCTCTTC | 1 cut(s) | 1045 |
| LmnI | GCTCC | 1 cut(s) | 964 |
| Lsp1109I | GCAGC | 6 cut(s) | 185, 346, 560, 563, 1138, 1151 |
| LweI | GCATC | 7 cut(s) | 208, 241, 263, 513, 892, 979, 1480 |
| MaeI | CTAG | 2 cut(s) | 1044, 1415 |
| MaeII | ACGT | 2 cut(s) | 1002, 1354 |
| MaeIII | GTNAC | 3 cut(s) | 266, 642, 1210 |
| MalI | GATC | 6 cut(s) | 36, 108, 371, 424, 446, 1328 |
| MboI | GATC | 6 cut(s) | 34, 106, 369, 422, 444, 1326 |
| MboII | GAAGA | 6 cut(s) | 205, 377, 445, 1032, 1330, 1581 |
| MfeI | CAATTG | 1 cut(s) | 24 |
| MflI | RGATCY | 2 cut(s) | 369, 422 |
| MlsI | TGGCCA | 1 cut(s) | 283 |
| MluNI | TGGCCA | 1 cut(s) | 283 |
| Mox20I | TGGCCA | 1 cut(s) | 283 |
| Mph1103I | ATGCAT | 3 cut(s) | 256, 336, 820 |
| MroXI | GAANNNNTTC | 1 cut(s) | 1079 |
| MscI | TGGCCA | 1 cut(s) | 283 |
| MseI | TTAA | 5 cut(s) | 180, 230, 465, 756, 809 |
| MslI | CAYNNNNRTG | 1 cut(s) | 1280 |
| Msp20I | TGGCCA | 1 cut(s) | 283 |
| MspR9I | CCNGG | 1 cut(s) | 569 |
| MunI | CAATTG | 1 cut(s) | 24 |
| Mva1269I | GAATGC | 3 cut(s) | 442, 1305, 1597 |
| MvaI | CCWGG | 1 cut(s) | 569 |
| MwoI | GCNNNNNNNGC | 4 cut(s) | 557, 1066, 1136, 1148 |
| NdeI | CATATG | 1 cut(s) | 1132 |
| NdeII | GATC | 6 cut(s) | 34, 106, 369, 422, 444, 1326 |
| NlaIII | CATG | 8 cut(s) | 23, 57, 77, 135, 338, 822, 1019, 1528 |
| NlaIV | GGNNCC | 3 cut(s) | 424, 453, 966 |
| NmeAIII | GCCGAG | 1 cut(s) | 18 |
| NmuCI | GTSAC | 3 cut(s) | 266, 642, 1210 |
| NsiI | ATGCAT | 3 cut(s) | 256, 336, 820 |
| NspI | RCATGY | 2 cut(s) | 822, 1528 |
| PceI | AGGCCT | 1 cut(s) | 513 |
| PciI | ACATGT | 1 cut(s) | 1524 |
| PciSI | GCTCTTC | 1 cut(s) | 1045 |
| PctI | GAATGC | 3 cut(s) | 442, 1305, 1597 |
| PdmI | GAANNNNTTC | 1 cut(s) | 1079 |
| PfeI | GAWTC | 1 cut(s) | 1288 |
| PkrI | GCNGC | 6 cut(s) | 175, 361, 550, 553, 1141, 1153 |
| PpuMI | RGGWCCY | 1 cut(s) | 302 |
| PscI | ACATGT | 1 cut(s) | 1524 |
| Psp1406I | AACGTT | 1 cut(s) | 1354 |
| Psp5II | RGGWCCY | 1 cut(s) | 302 |
| Psp6I | CCWGG | 1 cut(s) | 567 |
| PspGI | CCWGG | 1 cut(s) | 567 |
| PspN4I | GGNNCC | 3 cut(s) | 424, 453, 966 |
| PspPI | GGNCC | 2 cut(s) | 120, 302 |
| PspPPI | RGGWCCY | 1 cut(s) | 302 |
| PstI | CTGCAG | 2 cut(s) | 511, 1156 |
| PsuI | RGATCY | 2 cut(s) | 369, 422 |
| RsaI | GTAC | 4 cut(s) | 50, 606, 631, 1240 |
| RsaNI | GTAC | 4 cut(s) | 49, 605, 630, 1239 |
| RseI | CAYNNNNRTG | 1 cut(s) | 1280 |
| SapI | GCTCTTC | 1 cut(s) | 1045 |
| SaqAI | TTAA | 5 cut(s) | 180, 230, 465, 756, 809 |
| SatI | GCNGC | 6 cut(s) | 174, 360, 549, 552, 1140, 1152 |
| Sau3AI | GATC | 6 cut(s) | 34, 106, 369, 422, 444, 1326 |
| Sau96I | GGNCC | 2 cut(s) | 120, 302 |
| ScaI | AGTACT | 1 cut(s) | 50 |
| ScrFI | CCNGG | 1 cut(s) | 569 |
| SfaNI | GCATC | 7 cut(s) | 208, 241, 263, 513, 892, 979, 1480 |
| SfcI | CTRYAG | 3 cut(s) | 507, 1070, 1152 |
| SinI | GGWCC | 2 cut(s) | 120, 302 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 1280 |
| SmlI | CTYRAG | 2 cut(s) | 373, 1504 |
| SmoI | CTYRAG | 2 cut(s) | 373, 1504 |
| SseBI | AGGCCT | 1 cut(s) | 513 |
| SsiI | CCGC | 2 cut(s) | 226, 397 |
| SspMI | CTAG | 2 cut(s) | 1044, 1415 |
| StuI | AGGCCT | 1 cut(s) | 513 |
| StyD4I | CCNGG | 1 cut(s) | 567 |
| StyI | CCWWGG | 1 cut(s) | 1053 |
| TaaI | ACNGT | 5 cut(s) | 354, 430, 451, 743, 1562 |
| TaiI | ACGT | 2 cut(s) | 1005, 1357 |
| TaqI | TCGA | 2 cut(s) | 216, 720 |
| TatI | WGTACW | 3 cut(s) | 48, 604, 1238 |
| TfiI | GAWTC | 1 cut(s) | 1288 |
| Tru1I | TTAA | 5 cut(s) | 180, 230, 465, 756, 809 |
| Tru9I | TTAA | 5 cut(s) | 180, 230, 465, 756, 809 |
| TscAI | CASTG | 7 cut(s) | 151, 253, 359, 433, 691, 787, 1567 |
| TseFI | GTSAC | 3 cut(s) | 266, 642, 1210 |
| TseI | GCWGC | 6 cut(s) | 173, 359, 548, 551, 1139, 1151 |
| Tsp45I | GTSAC | 3 cut(s) | 266, 642, 1210 |
| TspDTI | ATGAA | 3 cut(s) | 351, 552, 834 |
| TspGWI | ACGGA | 2 cut(s) | 117, 1166 |
| TspRI | CASTG | 7 cut(s) | 151, 253, 359, 433, 691, 787, 1567 |
| VpaK11BI | GGWCC | 2 cut(s) | 120, 302 |
| XapI | RAATTY | 5 cut(s) | 242, 752, 923, 1047, 1336 |
| XbaI | TCTAGA | 1 cut(s) | 1043 |
| XceI | RCATGY | 2 cut(s) | 822, 1528 |
| XmnI | GAANNNNTTC | 1 cut(s) | 1079 |
| XspI | CTAG | 2 cut(s) | 1044, 1415 |
| ZrmI | AGTACT | 1 cut(s) | 50 |
| Zsp2I | ATGCAT | 3 cut(s) | 256, 336, 820 |