FvH4_7g22130
HSP70 Family

Heat shock cognate 70 kDa

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0000026
Physical Location & Seq
Reverse (-)
17523724 .. 17526772
3049 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g22130.t1

Sequence Viewer

Length: 1950 bp
ATGACCGGAAAAGGAGAGGGACCGGCGATTGGAATCGACCTCGGCACCACTTATTCTTGCGTCGGAGTGTGGCAGCACGACCGTGTTGAGATCATAGCCAACGATCAGGGCAACCGAACAACGCCGTCTTATGTGGCGTTCACTGACACCGAGCGTTTGATCGGTGATGCTGCAAAGAACCAGGTCGCCATGAACCCTATCAACACCGTCTTTGATGCTAAGCGGTTGATTGGAAGGAGATTTAGCGACTCTTCTGTTCAGGGTGACATGAAGCTATGGCCTTTCAAGCTCACTGCTGGCCCTAGTGACAAGCCGATGATTACAGTAAGTTACAAAGCGGAGGACAAACAGTTTGCTGCGGAAGAGATTTCATCTATGGTTCTTATTAAGATGAGGGAGATTGCAGAGGCTTATCTCGGCTCATCAATCAAGAATGCAGTTGTGACTGTGCCTGCTTACTTCAATGATTCTCAGAGACAGGCTACAAAGGATGCTGGTGTCATTGCTGGCCTTAATGTGATGCGTATTATCAATGAGCCTACAGCTGCTGCCATTGCATACGGTCTTGACAAGAAGGCAACTAGTGTGGGAGAGAAGAATGTGTTGATTTTCGATCTTGGTGGAGGTACTTTTGATGTCTCTCTACTTACCATTGAAGAAGGTATCTTTGAGGTGAAGTCTACTGCTGGAGATACCCATTTGGGTGGAGAGGATTTTGATAATAGGATGGTGAACCATTTTGTTCAAGAGTTTAAGAGGAAGAACAAGAAGGACATCAGTGGAAACCCAAGAGCTCTTAGGAGGTTGAGGACATCTTGTGAAAGAGCAAAGAGGACTCTCTCATCCACTGCACAGACTACAATTGAGATTGACTCCCTGTTTGAGGGTATCGATTTTTATTCAACCATTACGCGTGCTAGGTTTGAAGAGCTTAACATGGATTTGTTTAGGAAGTGTATGGAACCAGTTGAGAAGTGTTTGAGGGATGCGAAGATGGACAAGAGCACAGTCCATGATGTTGTACTTGTGGGTGGTTCTACCAGGATTCCTAAAGTACAGCAATTGTTGCAAGACTTCTTCAACGGAAAGGAACTGTGCAAAAGCATCAACCCAGACGAAGCTGTTGCTTATGGAGCTGCTGTGCAGGCTGCTATTCTTAGCGGTGAGGGTAATGAGAAGGTGCAGGACCTATTGCTTCTTGATGTGACTCCTCTGTCCTTGGGTTTGGAAACAGCTGGAGGTGTGATGACTGTGTTGATTCCGAGGAATACAACCATTCCAACCAAGAAAGAGCAAGTGTTCTCTACTTATTCAGATAATCAGCCTGGTGTCTTGATCCAGGTTTATGAGGGTGAGCGGACAAGGACTCGTGACAACAATTTGCTCGGCAAGTTTGAGCTCTCTGGCATTCCTCCAGCTCCTAGGGGAGTCCCTCAGATTACTGTTTGCTTTGACATTGACGCCAATGGCATTCTCAATGTGTCTGCTGAGGACAAGACCACAGGGCAGAAGAACAAAATTACTATTACCAATGACAAGGGAAGGCTGTCCAAAGAAGAGATTGAGAAGATGGTCCAAGAGGCTGAGAAGTACAAAGCAGAAGACGAGGATCACAAGAAGAAGGTCGAGGCTAAGAATGCTCTTGAGAACTACGCTTACAACATGAGGAACACCATCAAGGATGACAAGATTGCATCAAAGATTGACCCATCAGACAAAAAGAAGATTGAAGATGCAATTGAGGAGGCCATTCACTGGCTCGATAATAATCAGCTAGCAGAGGCTGATGAATTCGAGGACAAGATGAAGGAGCTTGAGAGTCTTTGCAACCCTATCATTGCCAAGATGTACCAGGGTGGTGCTGCTCCTGACATGAGTGGTGGAGCGGATGCTGATGTTCCTCCTTCTGGTGGAAGTGGTGCTGGTCCGAAGATTGAAGAAGTCGACTAA

Protein Analysis

650

Amino Acids

71.22

Weight (kDa)

5.13

Isoelectric Point (pI)

34.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HSP70 PF00012 9 - 618 6.6e-262 Hsp70 protein
MreB_Mbl PF06723 142 - 380 1.8e-13 MreB/Mbl protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000026)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G56410 AT3G09440 AT3G09440 AT3G09440 AT3G09440 AT3G12580 AT5G02490 AT5G02500 AT5G02500
fragaria_vesca FvH4_2g01420 FvH4_2g01790 FvH4_2g01870 FvH4_2g01900 FvH4_2g04440 FvH4_2g04830 FvH4_2g04842 FvH4_2g07600 FvH4_4g22920 FvH4_5g00700 FvH4_5g00710 FvH4_5g00890 FvH4_5g34480 FvH4_5g34480 FvH4_6g14090 FvH4_6g14090 FvH4_6g14090 FvH4_6g14580 FvH4_6g14580 FvH4_6g14580 FvH4_6g14580 FvH4_6g41130 FvH4_7g09000 FvH4_7g09000 FvH4_7g17120 FvH4_7g17140 FvH4_7g17150 FvH4_7g17151 FvH4_7g17152 FvH4_7g17153 FvH4_7g22130 FvH4_7g22170 FvH4_7g22240 FvH4_7g22240 FvH4_7g22240 FvH4_7g22250 FvH4_7g22260 FvH4_7g22281 FvH4_7g22282 FvH4_7g22283 FvH4_7g22284 FvH4_7g22285 FvH4_7g22285 FvH4_7g22320 FvH4_7g22331 FvH4_7g22332 FvH4_7g22333 FvH4_7g22333 FvH4_7g22360 FvH4_7g22361 FvH4_7g22362 FvH4_7g22363 FvH4_7g22370 FvH4_7g22371 FvH4_7g22380 FvH4_7g22380 FvH4_7g22380
malus_domestica MD00G1207500.v1.1 MD01G1126500.v1.1 MD01G1126900.v1.1 MD01G1127200.v1.1 MD01G1127300.v1.1 MD01G1127400.v1.1 MD01G1127500.v1.1 MD01G1127600.v1.1 MD01G1127700.v1.1 MD01G1128000.v1.1 MD01G1128300.v1.1 MD01G1128500.v1.1 MD01G1128800.v1.1 MD01G1129200.v1.1 MD01G1129500.v1.1 MD01G1129700.v1.1 MD01G1129800.v1.1 MD01G1130000.v1.1 MD01G1130200.v1.1 MD01G1130300.v1.1 MD01G1130400.v1.1 MD01G1133100.v1.1 MD01G1133300.v1.1 MD01G1133400.v1.1 MD04G1125600.v1.1 MD07G1196500.v1.1 MD07G1196700.v1.1 MD07G1196800.v1.1 MD07G1197700.v1.1 MD11G1111400.v1.1 MD12G1025600.v1.1 MD12G1025700.v1.1 MD12G1025800.v1.1 MD12G1025900.v1.1 MD12G1026000.v1.1 MD14G1024500.v1.1 MD14G1024700.v1.1 MD17G1263000.v1.1
prunus_persica Prupe.2G233500_v2.0.a1 Prupe.2G234200_v2.0.a1 Prupe.2G234200_v2.0.a1 Prupe.2G234300_v2.0.a1 Prupe.2G234400_v2.0.a1 Prupe.6G079800_v2.0.a1 Prupe.6G252500_v2.0.a1 Prupe.7G106500_v2.0.a1 Prupe.7G106700_v2.0.a1 Prupe.7G107400_v2.0.a1 Prupe.7G107400_v2.0.a1 Prupe.7G107400_v2.0.a1 Prupe.7G107700_v2.0.a1 Prupe.7G107700_v2.0.a1 Prupe.7G107800_v2.0.a1 Prupe.7G108100_v2.0.a1 Prupe.7G108500_v2.0.a1
pyrus_communis pycom01g15230 pycom01g15270 pycom01g15310 pycom01g15320 pycom01g15340 pycom01g15410 pycom01g15420 pycom02g00470 pycom07g18460 pycom07g18470 pycom11g09200 pycom12g02530 pycom14g02400 pycom14g02410
rosa_chinensis RchiOBHm_Chr1g0344521 RchiOBHm_Chr1g0360541 RchiOBHm_Chr1g0360551 RchiOBHm_Chr1g0360561 RchiOBHm_Chr1g0365061 RchiOBHm_Chr1g0366521 RchiOBHm_Chr1g0366531 RchiOBHm_Chr1g0366641 RchiOBHm_Chr1g0366651 RchiOBHm_Chr1g0366671 RchiOBHm_Chr1g0366681 RchiOBHm_Chr1g0366691 RchiOBHm_Chr1g0366701 RchiOBHm_Chr1g0366711 RchiOBHm_Chr1g0366721 RchiOBHm_Chr1g0366731 RchiOBHm_Chr1g0366741 RchiOBHm_Chr1g0366751 RchiOBHm_Chr1g0366761 RchiOBHm_Chr1g0366801 RchiOBHm_Chr1g0366811 RchiOBHm_Chr1g0366841 RchiOBHm_Chr1g0366851 RchiOBHm_Chr1g0366861 RchiOBHm_Chr1g0366921 RchiOBHm_Chr1g0366931 RchiOBHm_Chr1g0367001 RchiOBHm_Chr2g0129341 RchiOBHm_Chr3g0466971 RchiOBHm_Chr3g0467631 RchiOBHm_Chr3g0467651 RchiOBHm_Chr4g0396291 RchiOBHm_Chr4g0419751 RchiOBHm_Chr4g0419771 RchiOBHm_Chr6g0244031 RchiOBHm_Chr6g0244041 RchiOBHm_Chr6g0244641 RchiOBHm_Chr6g0244811 RchiOBHm_Chr6g0244881 RchiOBHm_Chr6g0245051 RchiOBHm_Chr6g0245081 RchiOBHm_Chr6g0251971 RchiOBHm_Chr6g0252041 RchiOBHm_Chr6g0252111 RchiOBHm_Chr6g0252141 RchiOBHm_Chr6g0252161 RchiOBHm_Chr6g0252171 RchiOBHm_Chr6g0252381 RchiOBHm_Chr6g0252391 RchiOBHm_Chr7g0200651 RchiOBHm_Chr7g0200731 RchiOBHm_Chr7g0200831 RchiOBHm_Chr7g0200941 RchiOBHm_Chr7g0201001 RchiOBHm_Chr7g0201141 RchiOBHm_Chr7g0201161 RchiOBHm_Chr7g0201431 RchiOBHm_Chr7g0201501 RchiOBHm_Chr7g0201511 RchiOBHm_Chr7g0235101 RchiOBHm_Chr7g0242841 RchiOBHm_Chr7g0242851
rosa_laevigata RLG00000000872 RLG00000000875 RLG00000000876 RLG00000001183 RLG00000003666 RLG00000003668 RLG00000003676 RLG00000003684 RLG00000003689 RLG00000003699 RLG00000003703 RLG00000003713 RLG00000003715 RLG00000003719 RLG00000007799 RLG00000014347 RLG00000015325 RLG00000015388 RLG00000015389 RLG00000015406 RLG00000015411 RLG00000015415 RLG00000024483 RLG00000024538 RLG00000027307 RLG00000027310 RLG00000027311 RLG00000027314 RLG00000027317 RLG00000027318 RLG00000027321 RLG00000027322 RLG00000027324 RLG00000027325 RLG00000027326 RLG00000027327 RLG00000027328 RLG00000027330 RLG00000027331 RLG00000027339 RLG00000027340 RLG00000027798 RLG00000028894
rosa_multiflora Rmu_co8077254.1_g000001 Rmu_co8228455.1_g000001 Rmu_co8251579.1_g000001 Rmu_co8383367.1_g000001 Rmu_sc0000016.1_g000010 Rmu_sc0000318.1_g000001 Rmu_sc0000318.1_g000011 Rmu_sc0000318.1_g000012 Rmu_sc0000318.1_g000018 Rmu_sc0000318.1_g000025 Rmu_sc0000318.1_g000027 Rmu_sc0000318.1_g000028 Rmu_sc0000318.1_g000029 Rmu_sc0000318.1_g000030 Rmu_sc0000318.1_g000036 Rmu_sc0000318.1_g000038 Rmu_sc0000318.1_g000039 Rmu_sc0000318.1_g000047 Rmu_sc0000318.1_g000049 Rmu_sc0000318.1_g000051 Rmu_sc0000708.1_g000013 Rmu_sc0000708.1_g000024 Rmu_sc0000785.1_g000007 Rmu_sc0001171.1_g000001 Rmu_sc0001193.1_g000010 Rmu_sc0001469.1_g000007 Rmu_sc0002046.1_g000003 Rmu_sc0002046.1_g000004 Rmu_sc0002269.1_g000006 Rmu_sc0002269.1_g000017 Rmu_sc0002269.1_g000018 Rmu_sc0002269.1_g000019 Rmu_sc0002269.1_g000023 Rmu_sc0002269.1_g000024 Rmu_sc0002522.1_g000041 Rmu_sc0002522.1_g000050 Rmu_sc0002522.1_g000053 Rmu_sc0002522.1_g000063 Rmu_sc0002952.1_g000018 Rmu_sc0003180.1_g000001 Rmu_sc0003180.1_g000003 Rmu_sc0003467.1_g000006 Rmu_sc0003467.1_g000012 Rmu_sc0003467.1_g000019 Rmu_sc0004165.1_g000049 Rmu_sc0004165.1_g000052 Rmu_sc0004248.1_g000011 Rmu_sc0004248.1_g000013 Rmu_sc0005230.1_g000018 Rmu_sc0006159.1_g000001 Rmu_sc0006159.1_g000003 Rmu_sc0006159.1_g000007 Rmu_sc0006159.1_g000008 Rmu_sc0006159.1_g000010 Rmu_sc0006159.1_g000011 Rmu_sc0006159.1_g000012 Rmu_sc0006159.1_g000014 Rmu_sc0006159.1_g000020 Rmu_sc0006159.1_g000022 Rmu_sc0006553.1_g000002 Rmu_sc0006553.1_g000006 Rmu_sc0006711.1_g000004 Rmu_sc0006711.1_g000005 Rmu_sc0007085.1_g000004 Rmu_sc0007349.1_g000004 Rmu_sc0011632.1_g000007 Rmu_sc0011632.1_g000008 Rmu_sc0011632.1_g000009 Rmu_sc0011883.1_g000003 Rmu_sc0011883.1_g000004 Rmu_sc0013714.1_g000004 Rmu_sc0016540.1_g000005 Rmu_sc0016540.1_g000006 Rmu_sc0016868.1_g000006 Rmu_sc0017656.1_g000003 Rmu_sc0018109.1_g000001 Rmu_sc0023871.1_g000001 Rmu_sc0030050.1_g000002 Rmu_sc0033254.1_g000001 Rmu_sc0033687.1_g000002 Rmu_sc0033687.1_g000003 Rmu_sc0035767.1_g000001 Rmu_sc0036983.1_g000002 Rmu_ssc0000087.1_g000012 Rmu_ssc0000087.1_g000017 Rmu_ssc0000087.1_g000020 Rmu_ssc0000087.1_g000054 Rmu_ssc0000087.1_g000061 Rmu_ssc0000087.1_g000069 Rmu_ssc0000112.1_g000012 Rmu_ssc0000250.1_g000030
rosa_roxburghii Rroxscaffold_3G00222340 Rroxscaffold_3G00222360 Rroxscaffold_3G00226300 Rroxscaffold_3G00255860 Rroxscaffold_4G00289710 Rroxscaffold_4G00289750 Rroxscaffold_4G00289800 Rroxscaffold_4G00289850 Rroxscaffold_4G00290110 Rroxscaffold_4G00291510 Rroxscaffold_4G00291960 Rroxscaffold_4G00309680 Rroxscaffold_6G00413530 Rroxscaffold_7G00215460 Rroxscaffold_7G00216130 Rroxscaffold_7G00216140
rosa_rugosa Rorug01G0174500 Rorug01G0279900 Rorug01G0319300 Rorug01G0330500 Rorug01G0330600 Rorug01G0331100 Rorug01G0331200 Rorug01G0331200 Rorug01G0331200 Rorug01G0331200 Rorug01G0331300 Rorug01G0331600 Rorug01G0331700 Rorug01G0332000 Rorug01G0332100 Rorug01G0332200 Rorug01G0332300 Rorug01G0332300 Rorug01G0332300 Rorug01G0332400 Rorug01G0332400 Rorug01G0332500 Rorug01G0332600 Rorug01G0332600 Rorug01G0333000 Rorug01G0333100 Rorug01G0333500 Rorug01G0334000 Rorug01G0334000 Rorug01G0334100 Rorug01G0334100 Rorug03G0000100 Rorug03G0084400 Rorug03G0088700 Rorug03G0088800 Rorug03G0088900 Rorug04G0160400 Rorug05G0441300 Rorug05G0506700 Rorug05G0506800.1 Rorug05G0511000 Rorug05G0511700 Rorug05G0511800 Rorug05G0511900 Rorug05G0512300 Rorug07G0061300 Rorug07G0062200.1 Rorug07G0064400 Rorug07G0064500 Rorug07G0107700 Rorug07G0287500 Rorug07G0317500 Rorug07G0317600
rosa_samantha Rh1BG157600 Rh1BG300200 Rh1CG176600 Rh1CG274600 Rh1CG315200 Rh1CG315300 Rh1CG315900 Rh1CG316300 Rh1CG316400 Rh1CG316600 Rh1CG316800 Rh1CG317000 Rh1CG317100 Rh1CG317200 Rh1CG317300 Rh1DG189000 Rh2DG363900 Rh3AG132500 Rh3AG137000 Rh3AG137100 Rh3CG153600 Rh3DG154600 Rh6BG014600 Rh6BG015100 Rh6BG016900 Rh6BG017100 Rh6BG020800 Rh6BG021900 Rh6BG119900 Rh7AG186900 Rh7AG187100 Rh7AG188200 Rh7AG188600 Rh7AG191000 Rh7AG191100 Rh7AG442500 Rh7AG472400 Rh7AG472800 Rh7BG415100 Rh7DG196000 Rh7DG196200 Rh7DG432200
rosa_wichuraiana Rw0G002960 Rw0G008590 Rw0G008610 Rw0G021810 Rw0G023900 Rw0G023910 Rw0G023920 Rw0G023930 Rw0G023940 Rw0G023950 Rw0G023960 Rw0G023970 Rw0G023980 Rw0G023990 Rw0G024000 Rw0G024010 Rw1G015730 Rw1G029130 Rw1G029980 Rw1G029990 Rw1G030100 Rw1G030110 Rw1G030120 Rw1G030130 Rw1G030140 Rw1G030150 Rw1G030160 Rw1G030170 Rw1G030180 Rw1G030210 Rw1G030230 Rw1G030240 Rw3G012520 Rw3G012540 Rw3G012980 Rw3G012990 Rw3G013000 Rw6G001750 Rw6G002200 Rw6G002270 Rw6G002280 Rw6G010820 Rw6G021710 Rw7G016340 Rw7G016370 Rw7G016420 Rw7G016550 Rw7G016600 Rw7G016750 Rw7G036790 Rw7G039110

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1213
AccB1I GGYRCC 1 cut(s) 44
AccB7I CCANNNNNTGG 1 cut(s) 1755
AccBSI CCGCTC 2 cut(s) 1357, 1886
AccI GTMKAC 2 cut(s) 680, 1944
AccII CGCG 1 cut(s) 913
AciI CCGC 6 cut(s) 223, 338, 359, 1161, 1357, 1886
AclWI GGATC 2 cut(s) 1330, 1617
AcsI RAATTY 1 cut(s) 1790
AcyI GRCGYC 1 cut(s) 1461
AfaI GTAC 5 cut(s) 628, 1023, 1056, 1592, 1850
AfiI CCNNNNNNNGG 5 cut(s) 29, 883, 1755, 1907, 1910
AflIII ACRYGT 1 cut(s) 911
AgsI TTSAA 9 cut(s) 286, 463, 656, 746, 903, 926, 1081, 1730, 1937
AhlI ACTAGT 1 cut(s) 581
AjnI CCWGG 5 cut(s) 180, 1040, 1324, 1338, 1851
AleI CACNNNNGTG 1 cut(s) 81
Alw21I GWGCWC 3 cut(s) 796, 1007, 1401
Alw26I GTCTC 2 cut(s) 469, 643
AlwI GGATC 2 cut(s) 1330, 1617
AlwNI CAGNNNCTG 2 cut(s) 548, 1784
AoxI GGCC 4 cut(s) 278, 298, 508, 1746
ApeKI GCWGC 8 cut(s) 73, 170, 356, 545, 548, 1136, 1148, 1862
ApoI RAATTY 1 cut(s) 1790
ArsI GACNNNNNNTTYG 4 cut(s) 335, 367, 863, 895
AspA2I CCTAGG 1 cut(s) 1421
AspS9I GGNCC 5 cut(s) 20, 299, 1186, 1573, 1925
AsuHPI GGTGA 6 cut(s) 176, 275, 685, 742, 1175, 1364
AsuNHI GCTAGC 1 cut(s) 1774
AvaII GGWCC 4 cut(s) 20, 1186, 1573, 1925
AvrII CCTAGG 1 cut(s) 1421
BanI GGYRCC 1 cut(s) 44
BanII GRGCYC 2 cut(s) 796, 1401
BauI CACGAG 1 cut(s) 1368
BbsI GAAGAC 1 cut(s) 1608
Bbv12I GWGCWC 3 cut(s) 796, 1007, 1401
BbvCI CCTCAGC 1 cut(s) 1488
BbvI GCAGC 8 cut(s) 85, 157, 343, 532, 535, 1123, 1135, 1849
BccI CCATC 5 cut(s) 721, 988, 1564, 1682, 1717
BceAI ACGGC 1 cut(s) 109
BcgI CGANNNNNNTGC 2 cut(s) 1106, 1140
BciT130I CCWGG 5 cut(s) 182, 1042, 1326, 1340, 1853
BcoDI GTCTC 2 cut(s) 469, 643
BcuI ACTAGT 1 cut(s) 581
BfaI CTAG 5 cut(s) 303, 582, 918, 1422, 1775
BfmI CTRYAG 1 cut(s) 540
BisI GCNGC 8 cut(s) 74, 171, 357, 546, 549, 1137, 1149, 1863
BlnI CCTAGG 1 cut(s) 1421
BlpI GCTNAGC 1 cut(s) 219
BlsI GCNGC 8 cut(s) 75, 172, 358, 547, 550, 1138, 1150, 1864
Bme1390I CCNGG 5 cut(s) 182, 1042, 1326, 1340, 1853
Bme18I GGWCC 4 cut(s) 20, 1186, 1573, 1925
BmgT120I GGNCC 5 cut(s) 20, 299, 1186, 1573, 1925
BmiI GGNNCC 3 cut(s) 21, 46, 963
BmrFI CCNGG 5 cut(s) 182, 1042, 1326, 1340, 1853
BmsI GCATC 9 cut(s) 157, 205, 481, 510, 976, 1113, 1703, 1723, 1879
BmtI GCTAGC 1 cut(s) 1778
BpiI GAAGAC 1 cut(s) 1608
BplI GAGNNNNNCTC 2 cut(s) 857, 889
BpmI CTGGAG 3 cut(s) 708, 1257, 1398
Bpu10I CCTNAGC 1 cut(s) 1488
Bpu1102I GCTNAGC 1 cut(s) 219
BpuEI CTTGAG 2 cut(s) 1664, 1835
Bsa29I ATCGAT 1 cut(s) 891
BsaBI GATNNNNATC 2 cut(s) 32, 1767
BsaHI GRCGYC 1 cut(s) 1461
BsaJI CCNNGG 5 cut(s) 40, 1218, 1262, 1421, 1852
BsaWI WCCGGW 1 cut(s) 5
BsaXI ACNNNNNCTCC 2 cut(s) 1125, 1155
Bsc4I CCNNNNNNNGG 5 cut(s) 29, 883, 1755, 1907, 1910
Bse118I RCCGGY 1 cut(s) 22
Bse1I ACTGG 2 cut(s) 965, 1760
Bse3DI GCAATG 3 cut(s) 501, 552, 1836
Bse8I GATNNNNATC 2 cut(s) 32, 1767
BseBI CCWGG 5 cut(s) 182, 1042, 1326, 1340, 1853
BseCI ATCGAT 1 cut(s) 891
BseDI CCNNGG 5 cut(s) 40, 1218, 1262, 1421, 1852
BseGI GGATG 6 cut(s) 496, 732, 842, 991, 1687, 1894
BseJI GATNNNNATC 2 cut(s) 32, 1767
BseLI CCNNNNNNNGG 5 cut(s) 29, 883, 1755, 1907, 1910
BseMI GCAATG 3 cut(s) 501, 552, 1836
BseMII CTCAG 4 cut(s) 485, 1448, 1479, 1575
BseNI ACTGG 2 cut(s) 965, 1760
BseRI GAGGAG 2 cut(s) 1200, 1757
BseXI GCAGC 8 cut(s) 85, 157, 343, 532, 535, 1123, 1135, 1849
BsgI GTGCAG 3 cut(s) 834, 1163, 1202
Bsh1236I CGCG 1 cut(s) 913
Bsh1285I CGRYCG 1 cut(s) 82
BshFI GGCC 4 cut(s) 280, 300, 510, 1748
BshNI GGYRCC 1 cut(s) 44
BshVI ATCGAT 1 cut(s) 891
BsiEI CGRYCG 1 cut(s) 82
BsiHKAI GWGCWC 3 cut(s) 796, 1007, 1401
BsiSI CCGG 2 cut(s) 6, 23
BslFI GGGAC 2 cut(s) 33, 1415
BslI CCNNNNNNNGG 5 cut(s) 29, 883, 1755, 1907, 1910
BsmAI GTCTC 2 cut(s) 469, 643
BsmFI GGGAC 2 cut(s) 33, 1415
BsmI GAATGC 4 cut(s) 439, 1407, 1470, 1642
BsnI GGCC 4 cut(s) 280, 300, 510, 1748
Bsp1286I GDGCHC 3 cut(s) 796, 1007, 1401
Bsp143I GATC 6 cut(s) 90, 103, 159, 613, 1335, 1609
Bsp1720I GCTNAGC 1 cut(s) 219
BspACI CCGC 6 cut(s) 223, 338, 359, 1161, 1357, 1886
BspANI GGCC 4 cut(s) 280, 300, 510, 1748
BspCNI CTCAG 4 cut(s) 484, 1447, 1480, 1576
BspDI ATCGAT 1 cut(s) 891
BspFNI CGCG 1 cut(s) 913
BspLI GGNNCC 3 cut(s) 21, 46, 963
BspOI GCTAGC 1 cut(s) 1778
BspPI GGATC 2 cut(s) 1330, 1617
BspQI GCTCTTC 1 cut(s) 921
BspT107I GGYRCC 1 cut(s) 44
BsrBI CCGCTC 2 cut(s) 1357, 1886
BsrDI GCAATG 3 cut(s) 501, 552, 1836
BsrFI RCCGGY 1 cut(s) 22
BsrI ACTGG 2 cut(s) 965, 1760
BssAI RCCGGY 1 cut(s) 22
BssECI CCNNGG 5 cut(s) 40, 1218, 1262, 1421, 1852
BssMI GATC 6 cut(s) 90, 103, 159, 613, 1335, 1609
BssNI GRCGYC 1 cut(s) 1461
BssSI CACGAG 1 cut(s) 1368
BssT1I CCWWGG 2 cut(s) 1218, 1421
Bst2BI CACGAG 1 cut(s) 1368
Bst2UI CCWGG 5 cut(s) 182, 1042, 1326, 1340, 1853
Bst6I CTCTTC 4 cut(s) 256, 357, 921, 1551
BstACI GRCGYC 1 cut(s) 1461
BstAPI GCANNNNNTGC 1 cut(s) 1066
BstC8I GCNNGC 6 cut(s) 298, 453, 508, 915, 1146, 1776
BstDEI CTNAG 8 cut(s) 219, 471, 797, 1157, 1434, 1488, 1584, 1632
BstENI CCTNNNNNAGG 1 cut(s) 881
BstF5I GGATG 6 cut(s) 496, 732, 842, 991, 1687, 1894
BstFNI CGCG 1 cut(s) 913
BstKTI GATC 6 cut(s) 93, 106, 162, 616, 1338, 1612
BstMAI GTCTC 2 cut(s) 469, 643
BstMBI GATC 6 cut(s) 90, 103, 159, 613, 1335, 1609
BstMCI CGRYCG 1 cut(s) 82
BstMWI GCNNNNNNNGC 6 cut(s) 286, 554, 1066, 1133, 1145, 1637
BstNI CCWGG 5 cut(s) 182, 1042, 1326, 1340, 1853
BstSCI CCNGG 5 cut(s) 180, 1040, 1324, 1338, 1851
BstSFI CTRYAG 1 cut(s) 540
BstUI CGCG 1 cut(s) 913
BstV1I GCAGC 8 cut(s) 85, 157, 343, 532, 535, 1123, 1135, 1849
BstV2I GAAGAC 1 cut(s) 1608
BstXI CCANNNNNNTGG 1 cut(s) 704
Bsu15I ATCGAT 1 cut(s) 891
BsuRI GGCC 4 cut(s) 280, 300, 510, 1748
BsuTUI ATCGAT 1 cut(s) 891
BtsCI GGATG 6 cut(s) 496, 732, 842, 991, 1687, 1894
BtsI GCAGTG 2 cut(s) 291, 846
BtsIMutI CAGTG 5 cut(s) 141, 291, 784, 846, 1753
Cac8I GCNNGC 6 cut(s) 298, 453, 508, 915, 1146, 1776
CaiI CAGNNNCTG 2 cut(s) 548, 1784
Cfr10I RCCGGY 1 cut(s) 22
Cfr13I GGNCC 5 cut(s) 20, 299, 1186, 1573, 1925
ClaI ATCGAT 1 cut(s) 891
CseI GACGC 2 cut(s) 49, 1469
CsiI ACCWGGT 1 cut(s) 180
Csp6I GTAC 5 cut(s) 627, 1022, 1055, 1591, 1849
CspCI CAANNNNNGTGG 2 cut(s) 567, 602
CviAII CATG 6 cut(s) 190, 268, 937, 1013, 1663, 1873
CviQI GTAC 5 cut(s) 627, 1022, 1055, 1591, 1849
DdeI CTNAG 8 cut(s) 219, 471, 797, 1157, 1434, 1488, 1584, 1632
DpnI GATC 6 cut(s) 92, 105, 161, 615, 1337, 1611
DpnII GATC 6 cut(s) 90, 103, 159, 613, 1335, 1609
DrdI GACNNNNNNGTC 1 cut(s) 1213
DseDI GACNNNNNNGTC 1 cut(s) 1213
Eam1104I CTCTTC 4 cut(s) 256, 357, 921, 1551
EarI CTCTTC 4 cut(s) 256, 357, 921, 1551
Ecl136II GAGCTC 2 cut(s) 794, 1399
Eco130I CCWWGG 2 cut(s) 1218, 1421
Eco24I GRGCYC 2 cut(s) 796, 1401
Eco47I GGWCC 4 cut(s) 20, 1186, 1573, 1925
Eco53kI GAGCTC 2 cut(s) 794, 1399
EcoICRI GAGCTC 2 cut(s) 794, 1399
EcoNI CCTNNNNNAGG 1 cut(s) 881
EcoO109I RGGNCCY 1 cut(s) 1186
EcoRI GAATTC 1 cut(s) 1790
EcoRII CCWGG 5 cut(s) 180, 1040, 1324, 1338, 1851
EcoT14I CCWWGG 2 cut(s) 1218, 1421
EcoT38I GRGCYC 2 cut(s) 796, 1401
ErhI CCWWGG 2 cut(s) 1218, 1421
FaeI CATG 6 cut(s) 193, 271, 940, 1016, 1666, 1876
FaqI GGGAC 2 cut(s) 33, 1415
FatI CATG 6 cut(s) 189, 267, 936, 1012, 1662, 1872
FblI GTMKAC 2 cut(s) 680, 1944
Fnu4HI GCNGC 8 cut(s) 74, 171, 357, 546, 549, 1137, 1149, 1863
FokI GGATG 6 cut(s) 503, 739, 829, 998, 1694, 1901
FriOI GRGCYC 2 cut(s) 796, 1401
Fsp4HI GCNGC 8 cut(s) 74, 171, 357, 546, 549, 1137, 1149, 1863
FspBI CTAG 5 cut(s) 303, 582, 918, 1422, 1775
GluI GCNGC 8 cut(s) 74, 171, 357, 546, 549, 1137, 1149, 1863
GsuI CTGGAG 3 cut(s) 708, 1257, 1398
HaeIII GGCC 4 cut(s) 280, 300, 510, 1748
HapII CCGG 2 cut(s) 6, 23
HgaI GACGC 2 cut(s) 49, 1469
Hin1I GRCGYC 1 cut(s) 1461
Hin1II CATG 6 cut(s) 193, 271, 940, 1016, 1666, 1876
HincII GTYRAC 1 cut(s) 1945
HindII GTYRAC 1 cut(s) 1945
HpaII CCGG 2 cut(s) 6, 23
HphI GGTGA 6 cut(s) 176, 275, 685, 742, 1175, 1364
Hpy166II GTNNAC 4 cut(s) 141, 681, 733, 1945
Hpy188I TCNGA 7 cut(s) 65, 474, 1263, 1315, 1437, 1714, 1929
Hpy188III TCNNGA 8 cut(s) 430, 566, 746, 1199, 1333, 1370, 1643, 1868
Hpy8I GTNNAC 4 cut(s) 141, 681, 733, 1945
Hpy99I CGWCG 1 cut(s) 65
HpyAV CCTTC 9 cut(s) 228, 568, 653, 763, 1171, 1536, 1615, 1801, 1914
HpyF10VI GCNNNNNNNGC 6 cut(s) 286, 554, 1066, 1133, 1145, 1637
HpyF3I CTNAG 8 cut(s) 219, 471, 797, 1157, 1434, 1488, 1584, 1632
Hsp92I GRCGYC 1 cut(s) 1461
Hsp92II CATG 6 cut(s) 193, 271, 940, 1016, 1666, 1876
Kzo9I GATC 6 cut(s) 90, 103, 159, 613, 1335, 1609
LguI GCTCTTC 1 cut(s) 921
LmnI GCTCC 5 cut(s) 1133, 1423, 1810, 1870, 1883
Lsp1109I GCAGC 8 cut(s) 85, 157, 343, 532, 535, 1123, 1135, 1849
LweI GCATC 9 cut(s) 157, 205, 481, 510, 976, 1113, 1703, 1723, 1879
MabI ACCWGGT 1 cut(s) 180
MaeI CTAG 5 cut(s) 303, 582, 918, 1422, 1775
MaeIII GTNAC 6 cut(s) 263, 305, 329, 442, 1204, 1370
MalI GATC 6 cut(s) 92, 105, 161, 615, 1337, 1611
MbiI CCGCTC 2 cut(s) 1357, 1886
MboI GATC 6 cut(s) 90, 103, 159, 613, 1335, 1609
MfeI CAATTG 3 cut(s) 861, 1061, 1737
MhlI GDGCHC 3 cut(s) 796, 1007, 1401
MluCI AATT 6 cut(s) 861, 1061, 1378, 1518, 1737, 1790
MluI ACGCGT 1 cut(s) 911
MlyI GAGTC 7 cut(s) 242, 829, 866, 1201, 1360, 1437, 1828
MmeI TCCRAC 2 cut(s) 43, 1304
MseI TTAA 4 cut(s) 387, 513, 753, 933
MslI CAYNNNNRTG 2 cut(s) 81, 702
MspA1I CMGCKG 2 cut(s) 545, 1235
MspI CCGG 2 cut(s) 6, 23
MspR9I CCNGG 5 cut(s) 182, 1042, 1326, 1340, 1853
MunI CAATTG 3 cut(s) 861, 1061, 1737
Mva1269I GAATGC 4 cut(s) 439, 1407, 1470, 1642
MvaI CCWGG 5 cut(s) 182, 1042, 1326, 1340, 1853
MvnI CGCG 1 cut(s) 913
MwoI GCNNNNNNNGC 6 cut(s) 286, 554, 1066, 1133, 1145, 1637
NdeII GATC 6 cut(s) 90, 103, 159, 613, 1335, 1609
NheI GCTAGC 1 cut(s) 1774
NlaIII CATG 6 cut(s) 193, 271, 940, 1016, 1666, 1876
NlaIV GGNNCC 3 cut(s) 21, 46, 963
NmeAIII GCCGAG 3 cut(s) 21, 396, 1365
NmuCI GTSAC 5 cut(s) 263, 305, 442, 1204, 1370
OliI CACNNNNGTG 1 cut(s) 81
PciSI GCTCTTC 1 cut(s) 921
PctI GAATGC 4 cut(s) 439, 1407, 1470, 1642
PfeI GAWTC 4 cut(s) 33, 467, 1045, 1258
PflMI CCANNNNNTGG 1 cut(s) 1755
PkrI GCNGC 8 cut(s) 75, 172, 358, 547, 550, 1138, 1150, 1864
PleI GAGTC 7 cut(s) 242, 829, 866, 1201, 1360, 1436, 1827
PpsI GAGTC 7 cut(s) 242, 829, 866, 1201, 1360, 1436, 1827
PpuMI RGGWCCY 1 cut(s) 1186
Psp124BI GAGCTC 2 cut(s) 796, 1401
Psp5II RGGWCCY 1 cut(s) 1186
Psp6I CCWGG 5 cut(s) 180, 1040, 1324, 1338, 1851
PspGI CCWGG 5 cut(s) 180, 1040, 1324, 1338, 1851
PspN4I GGNNCC 3 cut(s) 21, 46, 963
PspPI GGNCC 5 cut(s) 20, 299, 1186, 1573, 1925
PspPPI RGGWCCY 1 cut(s) 1186
PsrI GAACNNNNNNTAC 2 cut(s) 1640, 1672
PstNI CAGNNNCTG 2 cut(s) 548, 1784
PvuII CAGCTG 2 cut(s) 545, 1235
RsaI GTAC 5 cut(s) 628, 1023, 1056, 1592, 1850
RsaNI GTAC 5 cut(s) 627, 1022, 1055, 1591, 1849
RseI CAYNNNNRTG 2 cut(s) 81, 702
SacI GAGCTC 2 cut(s) 796, 1401
SalI GTCGAC 1 cut(s) 1943
SapI GCTCTTC 1 cut(s) 921
SaqAI TTAA 4 cut(s) 387, 513, 753, 933
SatI GCNGC 8 cut(s) 74, 171, 357, 546, 549, 1137, 1149, 1863
Sau3AI GATC 6 cut(s) 90, 103, 159, 613, 1335, 1609
Sau96I GGNCC 5 cut(s) 20, 299, 1186, 1573, 1925
SchI GAGTC 7 cut(s) 242, 829, 866, 1201, 1360, 1437, 1828
ScrFI CCNGG 5 cut(s) 182, 1042, 1326, 1340, 1853
SduI GDGCHC 3 cut(s) 796, 1007, 1401
SexAI ACCWGGT 1 cut(s) 180
SfaNI GCATC 9 cut(s) 157, 205, 481, 510, 976, 1113, 1703, 1723, 1879
SfcI CTRYAG 1 cut(s) 540
SinI GGWCC 4 cut(s) 20, 1186, 1573, 1925
SmiMI CAYNNNNRTG 2 cut(s) 81, 702
SmlI CTYRAG 2 cut(s) 1643, 1814
SmoI CTYRAG 2 cut(s) 1643, 1814
SpeI ACTAGT 1 cut(s) 581
Sse9I AATT 6 cut(s) 861, 1061, 1378, 1518, 1737, 1790
SsiI CCGC 6 cut(s) 223, 338, 359, 1161, 1357, 1886
SspMI CTAG 5 cut(s) 303, 582, 918, 1422, 1775
SstI GAGCTC 2 cut(s) 796, 1401
StyD4I CCNGG 5 cut(s) 180, 1040, 1324, 1338, 1851
StyI CCWWGG 2 cut(s) 1218, 1421
TaqI TCGA 7 cut(s) 36, 612, 891, 1626, 1761, 1794, 1944
TasI AATT 6 cut(s) 861, 1061, 1378, 1518, 1737, 1790
TatI WGTACW 3 cut(s) 1021, 1054, 1590
TfiI GAWTC 4 cut(s) 33, 467, 1045, 1258
Tru1I TTAA 4 cut(s) 387, 513, 753, 933
Tru9I TTAA 4 cut(s) 387, 513, 753, 933
TscAI CASTG 5 cut(s) 148, 298, 784, 853, 1760
TseFI GTSAC 5 cut(s) 263, 305, 442, 1204, 1370
TseI GCWGC 8 cut(s) 73, 170, 356, 545, 548, 1136, 1148, 1862
Tsp45I GTSAC 5 cut(s) 263, 305, 442, 1204, 1370
TspDTI ATGAA 5 cut(s) 206, 284, 360, 1803, 1820
TspGWI ACGGA 1 cut(s) 1098
TspRI CASTG 5 cut(s) 148, 298, 784, 853, 1760
Van91I CCANNNNNTGG 1 cut(s) 1755
VpaK11BI GGWCC 4 cut(s) 20, 1186, 1573, 1925
XagI CCTNNNNNAGG 1 cut(s) 881
XapI RAATTY 1 cut(s) 1790
XmaJI CCTAGG 1 cut(s) 1421
XmiI GTMKAC 2 cut(s) 680, 1944
XspI CTAG 5 cut(s) 303, 582, 918, 1422, 1775
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.