Belongs to the heat shock protein 70 family
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 1719 |
| AatII | GACGTC | 1 cut(s) | 672 |
| AciI | CCGC | 1 cut(s) | 82 |
| AclWI | GGATC | 5 cut(s) | 50, 436, 885, 953, 966 |
| AcoI | YGGCCR | 1 cut(s) | 1213 |
| AcsI | RAATTY | 4 cut(s) | 917, 937, 1041, 1362 |
| AcuI | CTGAAG | 2 cut(s) | 435, 1596 |
| AcyI | GRCGYC | 1 cut(s) | 669 |
| AfaI | GTAC | 3 cut(s) | 625, 966, 1321 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 1815 |
| AflIII | ACRYGT | 1 cut(s) | 433 |
| AjnI | CCWGG | 1 cut(s) | 189 |
| AjuI | GAANNNNNNNTTGG | 4 cut(s) | 408, 440, 749, 781 |
| AleI | CACNNNNGTG | 1 cut(s) | 699 |
| AloI | GAACNNNNNNTCC | 2 cut(s) | 1122, 1154 |
| Alw21I | GWGCWC | 3 cut(s) | 793, 1488, 1746 |
| Alw26I | GTCTC | 5 cut(s) | 401, 681, 826, 1573, 1829 |
| AlwI | GGATC | 5 cut(s) | 50, 436, 885, 953, 966 |
| Ama87I | CYCGRG | 1 cut(s) | 786 |
| AoxI | GGCC | 5 cut(s) | 192, 571, 585, 675, 1213 |
| ApeKI | GCWGC | 5 cut(s) | 179, 554, 557, 1133, 1145 |
| ApoI | RAATTY | 4 cut(s) | 917, 937, 1041, 1362 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 647, 679 |
| AseI | ATTAAT | 1 cut(s) | 236 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 941 |
| AspA2I | CCTAGG | 1 cut(s) | 649 |
| AsuC2I | CCSGG | 1 cut(s) | 1636 |
| AsuHPI | GGTGA | 9 cut(s) | 185, 665, 686, 716, 739, 1451, 1487, 1709, 1745 |
| AvaI | CYCGRG | 1 cut(s) | 786 |
| AvrII | CCTAGG | 1 cut(s) | 649 |
| BamHI | GGATCC | 1 cut(s) | 958 |
| BanII | GRGCYC | 2 cut(s) | 793, 1488 |
| BbsI | GAAGAC | 3 cut(s) | 356, 1584, 1806 |
| Bbv12I | GWGCWC | 3 cut(s) | 793, 1488, 1746 |
| BbvI | GCAGC | 5 cut(s) | 191, 541, 569, 1132, 1145 |
| BccI | CCATC | 3 cut(s) | 142, 203, 1376 |
| BceAI | ACGGC | 1 cut(s) | 1570 |
| BciT130I | CCWGG | 1 cut(s) | 191 |
| BclI | TGATCA | 1 cut(s) | 1300 |
| BcnI | CCSGG | 1 cut(s) | 1636 |
| BcoDI | GTCTC | 5 cut(s) | 401, 681, 826, 1573, 1829 |
| BfaI | CTAG | 2 cut(s) | 650, 1038 |
| BfmI | CTRYAG | 2 cut(s) | 555, 1146 |
| BisI | GCNGC | 5 cut(s) | 180, 555, 558, 1134, 1146 |
| BlnI | CCTAGG | 1 cut(s) | 649 |
| BlsI | GCNGC | 5 cut(s) | 181, 556, 559, 1135, 1147 |
| Bme1390I | CCNGG | 2 cut(s) | 191, 1636 |
| BmeT110I | CYCGRG | 1 cut(s) | 786 |
| BmiI | GGNNCC | 1 cut(s) | 960 |
| BmrFI | CCNGG | 2 cut(s) | 191, 1636 |
| BmsI | GCATC | 8 cut(s) | 166, 214, 247, 390, 519, 973, 1183, 1537 |
| BpiI | GAAGAC | 3 cut(s) | 356, 1584, 1806 |
| BpmI | CTGGAG | 4 cut(s) | 351, 705, 1485, 1743 |
| Bpu10I | CCTNAGC | 1 cut(s) | 1521 |
| BpuMI | CCSGG | 1 cut(s) | 1636 |
| BsaBI | GATNNNNATC | 1 cut(s) | 1823 |
| BsaHI | GRCGYC | 1 cut(s) | 669 |
| BsaJI | CCNNGG | 5 cut(s) | 261, 424, 649, 697, 1728 |
| BsaWI | WCCGGW | 1 cut(s) | 1380 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 1122, 1152 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 1815 |
| Bse1I | ACTGG | 2 cut(s) | 171, 1594 |
| Bse8I | GATNNNNATC | 1 cut(s) | 1823 |
| BseBI | CCWGG | 1 cut(s) | 191 |
| BseDI | CCNNGG | 5 cut(s) | 261, 424, 649, 697, 1728 |
| BseGI | GGATG | 5 cut(s) | 380, 1015, 1075, 1264, 1338 |
| BseJI | GATNNNNATC | 1 cut(s) | 1823 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 1815 |
| BseMII | CTCAG | 2 cut(s) | 1653, 1824 |
| BseNI | ACTGG | 2 cut(s) | 171, 1594 |
| BseRI | GAGGAG | 1 cut(s) | 844 |
| BseXI | GCAGC | 5 cut(s) | 191, 541, 569, 1132, 1145 |
| BshFI | GGCC | 5 cut(s) | 194, 573, 587, 677, 1215 |
| BsiHKAI | GWGCWC | 3 cut(s) | 793, 1488, 1746 |
| BsiHKCI | CYCGRG | 1 cut(s) | 786 |
| BsiSI | CCGG | 3 cut(s) | 1216, 1381, 1635 |
| BslFI | GGGAC | 1 cut(s) | 443 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 1815 |
| BsmAI | GTCTC | 5 cut(s) | 401, 681, 826, 1573, 1829 |
| BsmFI | GGGAC | 1 cut(s) | 443 |
| BsmI | GAATGC | 3 cut(s) | 448, 505, 1557 |
| BsnI | GGCC | 5 cut(s) | 194, 573, 587, 677, 1215 |
| BsoBI | CYCGRG | 1 cut(s) | 786 |
| Bsp1286I | GDGCHC | 3 cut(s) | 793, 1488, 1746 |
| Bsp1407I | TGTACA | 1 cut(s) | 964 |
| Bsp143I | GATC | 7 cut(s) | 42, 112, 428, 877, 958, 1300, 1327 |
| Bsp19I | CCATGG | 1 cut(s) | 1728 |
| BspACI | CCGC | 1 cut(s) | 82 |
| BspANI | GGCC | 5 cut(s) | 194, 573, 587, 677, 1215 |
| BspCNI | CTCAG | 2 cut(s) | 1654, 1825 |
| BspHI | TCATGA | 1 cut(s) | 1324 |
| BspLI | GGNNCC | 1 cut(s) | 960 |
| BspMAI | CTGCAG | 2 cut(s) | 559, 1150 |
| BspPI | GGATC | 5 cut(s) | 50, 436, 885, 953, 966 |
| BspQI | GCTCTTC | 1 cut(s) | 1039 |
| BsrGI | TGTACA | 1 cut(s) | 964 |
| BsrI | ACTGG | 2 cut(s) | 171, 1594 |
| BssECI | CCNNGG | 5 cut(s) | 261, 424, 649, 697, 1728 |
| BssMI | GATC | 7 cut(s) | 42, 112, 428, 877, 958, 1300, 1327 |
| BssNI | GRCGYC | 1 cut(s) | 669 |
| BssT1I | CCWWGG | 4 cut(s) | 424, 649, 697, 1728 |
| Bst2UI | CCWGG | 1 cut(s) | 191 |
| Bst4CI | ACNGT | 5 cut(s) | 360, 457, 659, 872, 1004 |
| Bst6I | CTCTTC | 2 cut(s) | 18, 1039 |
| BstACI | GRCGYC | 1 cut(s) | 669 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 563 |
| BstAUI | TGTACA | 1 cut(s) | 964 |
| BstC8I | GCNNGC | 1 cut(s) | 988 |
| BstDEI | CTNAG | 5 cut(s) | 1521, 1620, 1662, 1779, 1833 |
| BstDSI | CCRYGG | 1 cut(s) | 1728 |
| BstF5I | GGATG | 5 cut(s) | 380, 1015, 1075, 1264, 1338 |
| BstKTI | GATC | 7 cut(s) | 45, 115, 431, 880, 961, 1303, 1330 |
| BstMAI | GTCTC | 5 cut(s) | 401, 681, 826, 1573, 1829 |
| BstMBI | GATC | 7 cut(s) | 42, 112, 428, 877, 958, 1300, 1327 |
| BstMWI | GCNNNNNNNGC | 4 cut(s) | 563, 987, 1130, 1142 |
| BstNI | CCWGG | 1 cut(s) | 191 |
| BstNSI | RCATGY | 2 cut(s) | 437, 1775 |
| BstSCI | CCNGG | 2 cut(s) | 189, 1634 |
| BstSFI | CTRYAG | 2 cut(s) | 555, 1146 |
| BstV1I | GCAGC | 5 cut(s) | 191, 541, 569, 1132, 1145 |
| BstV2I | GAAGAC | 3 cut(s) | 356, 1584, 1806 |
| BstX2I | RGATCY | 1 cut(s) | 958 |
| BstYI | RGATCY | 1 cut(s) | 958 |
| BsuRI | GGCC | 5 cut(s) | 194, 573, 587, 677, 1215 |
| BtgI | CCRYGG | 1 cut(s) | 1728 |
| BtgZI | GCGATG | 2 cut(s) | 270, 996 |
| BtsCI | GGATG | 5 cut(s) | 380, 1015, 1075, 1264, 1338 |
| BtsIMutI | CAGTG | 5 cut(s) | 150, 453, 1009, 1587, 1830 |
| Cac8I | GCNNGC | 1 cut(s) | 988 |
| CciI | TCATGA | 1 cut(s) | 1324 |
| Csp6I | GTAC | 3 cut(s) | 624, 965, 1320 |
| CviAII | CATG | 9 cut(s) | 272, 385, 434, 934, 991, 1325, 1729, 1772, 1846 |
| CviQI | GTAC | 3 cut(s) | 624, 965, 1320 |
| DdeI | CTNAG | 5 cut(s) | 1521, 1620, 1662, 1779, 1833 |
| DpnI | GATC | 7 cut(s) | 44, 114, 430, 879, 960, 1302, 1329 |
| DpnII | GATC | 7 cut(s) | 42, 112, 428, 877, 958, 1300, 1327 |
| EaeI | YGGCCR | 1 cut(s) | 1213 |
| Eam1104I | CTCTTC | 2 cut(s) | 18, 1039 |
| EarI | CTCTTC | 2 cut(s) | 18, 1039 |
| EciI | GGCGGA | 1 cut(s) | 97 |
| Ecl136II | GAGCTC | 2 cut(s) | 791, 1486 |
| Eco130I | CCWWGG | 4 cut(s) | 424, 649, 697, 1728 |
| Eco24I | GRGCYC | 2 cut(s) | 793, 1488 |
| Eco53kI | GAGCTC | 2 cut(s) | 791, 1486 |
| Eco57I | CTGAAG | 2 cut(s) | 435, 1596 |
| Eco88I | CYCGRG | 1 cut(s) | 786 |
| EcoICRI | GAGCTC | 2 cut(s) | 791, 1486 |
| EcoRI | GAATTC | 2 cut(s) | 1041, 1362 |
| EcoRII | CCWGG | 1 cut(s) | 189 |
| EcoT14I | CCWWGG | 4 cut(s) | 424, 649, 697, 1728 |
| EcoT38I | GRGCYC | 2 cut(s) | 793, 1488 |
| ErhI | CCWWGG | 4 cut(s) | 424, 649, 697, 1728 |
| FaeI | CATG | 9 cut(s) | 275, 388, 437, 937, 994, 1328, 1732, 1775, 1849 |
| FalI | AAGNNNNNCTT | 4 cut(s) | 408, 440, 796, 828 |
| FaqI | GGGAC | 1 cut(s) | 443 |
| FatI | CATG | 9 cut(s) | 271, 384, 433, 933, 990, 1324, 1728, 1771, 1845 |
| FauNDI | CATATG | 2 cut(s) | 1126, 1642 |
| FbaI | TGATCA | 1 cut(s) | 1300 |
| Fnu4HI | GCNGC | 5 cut(s) | 180, 555, 558, 1134, 1146 |
| FokI | GGATG | 5 cut(s) | 367, 1022, 1082, 1271, 1345 |
| FriOI | GRGCYC | 2 cut(s) | 793, 1488 |
| Fsp4HI | GCNGC | 5 cut(s) | 180, 555, 558, 1134, 1146 |
| FspBI | CTAG | 2 cut(s) | 650, 1038 |
| GluI | GCNGC | 5 cut(s) | 180, 555, 558, 1134, 1146 |
| GsuI | CTGGAG | 4 cut(s) | 351, 705, 1485, 1743 |
| HaeIII | GGCC | 5 cut(s) | 194, 573, 587, 677, 1215 |
| HapII | CCGG | 3 cut(s) | 1216, 1381, 1635 |
| Hin1I | GRCGYC | 1 cut(s) | 669 |
| Hin1II | CATG | 9 cut(s) | 275, 388, 437, 937, 994, 1328, 1732, 1775, 1849 |
| HindIII | AAGCTT | 2 cut(s) | 417, 1523 |
| HinfI | GANTC | 4 cut(s) | 328, 388, 1219, 1345 |
| HpaII | CCGG | 3 cut(s) | 1216, 1381, 1635 |
| HphI | GGTGA | 9 cut(s) | 185, 665, 686, 716, 739, 1451, 1487, 1709, 1745 |
| Hpy166II | GTNNAC | 3 cut(s) | 1000, 1587, 1809 |
| Hpy188I | TCNGA | 4 cut(s) | 200, 333, 1047, 1332 |
| Hpy188III | TCNNGA | 9 cut(s) | 368, 751, 786, 1038, 1109, 1190, 1241, 1325, 1372 |
| Hpy8I | GTNNAC | 3 cut(s) | 1000, 1587, 1809 |
| HpyAV | CCTTC | 7 cut(s) | 157, 299, 577, 1042, 1078, 1430, 1688 |
| HpyCH4III | ACNGT | 5 cut(s) | 360, 457, 659, 872, 1004 |
| HpyCH4IV | ACGT | 5 cut(s) | 141, 484, 669, 771, 1411 |
| HpyF10VI | GCNNNNNNNGC | 4 cut(s) | 563, 987, 1130, 1142 |
| HpyF3I | CTNAG | 5 cut(s) | 1521, 1620, 1662, 1779, 1833 |
| HpySE526I | ACGT | 5 cut(s) | 141, 484, 669, 771, 1411 |
| Hsp92I | GRCGYC | 1 cut(s) | 669 |
| Hsp92II | CATG | 9 cut(s) | 275, 388, 437, 937, 994, 1328, 1732, 1775, 1849 |
| Ksp22I | TGATCA | 1 cut(s) | 1300 |
| Kzo9I | GATC | 7 cut(s) | 42, 112, 428, 877, 958, 1300, 1327 |
| LguI | GCTCTTC | 1 cut(s) | 1039 |
| LmnI | GCTCC | 4 cut(s) | 370, 1130, 1510, 1768 |
| Lsp1109I | GCAGC | 5 cut(s) | 191, 541, 569, 1132, 1145 |
| LweI | GCATC | 8 cut(s) | 166, 214, 247, 390, 519, 973, 1183, 1537 |
| MaeI | CTAG | 2 cut(s) | 650, 1038 |
| MaeII | ACGT | 5 cut(s) | 141, 484, 669, 771, 1411 |
| MaeIII | GTNAC | 4 cut(s) | 636, 653, 1261, 1681 |
| MalI | GATC | 7 cut(s) | 44, 114, 430, 879, 960, 1302, 1329 |
| MboI | GATC | 7 cut(s) | 42, 112, 428, 877, 958, 1300, 1327 |
| MfeI | CAATTG | 2 cut(s) | 561, 852 |
| MflI | RGATCY | 1 cut(s) | 958 |
| MhlI | GDGCHC | 3 cut(s) | 793, 1488, 1746 |
| MroXI | GAANNNNTTC | 1 cut(s) | 941 |
| MslI | CAYNNNNRTG | 3 cut(s) | 699, 995, 1373 |
| MspA1I | CMGCKG | 3 cut(s) | 554, 683, 1136 |
| MspI | CCGG | 3 cut(s) | 1216, 1381, 1635 |
| MspR9I | CCNGG | 2 cut(s) | 191, 1636 |
| MunI | CAATTG | 2 cut(s) | 561, 852 |
| Mva1269I | GAATGC | 3 cut(s) | 448, 505, 1557 |
| MvaI | CCWGG | 1 cut(s) | 191 |
| MwoI | GCNNNNNNNGC | 4 cut(s) | 563, 987, 1130, 1142 |
| NciI | CCSGG | 1 cut(s) | 1636 |
| NcoI | CCATGG | 1 cut(s) | 1728 |
| NdeI | CATATG | 2 cut(s) | 1126, 1642 |
| NdeII | GATC | 7 cut(s) | 42, 112, 428, 877, 958, 1300, 1327 |
| NlaIII | CATG | 9 cut(s) | 275, 388, 437, 937, 994, 1328, 1732, 1775, 1849 |
| NlaIV | GGNNCC | 1 cut(s) | 960 |
| NmuCI | GTSAC | 3 cut(s) | 636, 653, 1261 |
| NspI | RCATGY | 2 cut(s) | 437, 1775 |
| OliI | CACNNNNGTG | 1 cut(s) | 699 |
| PagI | TCATGA | 1 cut(s) | 1324 |
| PciI | ACATGT | 1 cut(s) | 433 |
| PciSI | GCTCTTC | 1 cut(s) | 1039 |
| PctI | GAATGC | 3 cut(s) | 448, 505, 1557 |
| PdmI | GAANNNNTTC | 1 cut(s) | 941 |
| PfeI | GAWTC | 4 cut(s) | 328, 388, 1219, 1345 |
| PkrI | GCNGC | 5 cut(s) | 181, 556, 559, 1135, 1147 |
| PscI | ACATGT | 1 cut(s) | 433 |
| PshBI | ATTAAT | 1 cut(s) | 236 |
| PsiI | TTATAA | 1 cut(s) | 1719 |
| Psp124BI | GAGCTC | 2 cut(s) | 793, 1488 |
| Psp6I | CCWGG | 1 cut(s) | 189 |
| PspGI | CCWGG | 1 cut(s) | 189 |
| PspN4I | GGNNCC | 1 cut(s) | 960 |
| PstI | CTGCAG | 2 cut(s) | 559, 1150 |
| PsuI | RGATCY | 1 cut(s) | 958 |
| PvuII | CAGCTG | 3 cut(s) | 554, 683, 1136 |
| RsaI | GTAC | 3 cut(s) | 625, 966, 1321 |
| RsaNI | GTAC | 3 cut(s) | 624, 965, 1320 |
| RseI | CAYNNNNRTG | 3 cut(s) | 699, 995, 1373 |
| SacI | GAGCTC | 2 cut(s) | 793, 1488 |
| SapI | GCTCTTC | 1 cut(s) | 1039 |
| SatI | GCNGC | 5 cut(s) | 180, 555, 558, 1134, 1146 |
| Sau3AI | GATC | 7 cut(s) | 42, 112, 428, 877, 958, 1300, 1327 |
| ScrFI | CCNGG | 2 cut(s) | 191, 1636 |
| SduI | GDGCHC | 3 cut(s) | 793, 1488, 1746 |
| SfaNI | GCATC | 8 cut(s) | 166, 214, 247, 390, 519, 973, 1183, 1537 |
| SfcI | CTRYAG | 2 cut(s) | 555, 1146 |
| SmiMI | CAYNNNNRTG | 3 cut(s) | 699, 995, 1373 |
| SsiI | CCGC | 1 cut(s) | 82 |
| SspMI | CTAG | 2 cut(s) | 650, 1038 |
| SstI | GAGCTC | 2 cut(s) | 793, 1488 |
| StyD4I | CCNGG | 2 cut(s) | 189, 1634 |
| StyI | CCWWGG | 4 cut(s) | 424, 649, 697, 1728 |
| TaaI | ACNGT | 5 cut(s) | 360, 457, 659, 872, 1004 |
| TaiI | ACGT | 5 cut(s) | 144, 487, 672, 774, 1414 |
| TaqI | TCGA | 1 cut(s) | 45 |
| TatI | WGTACW | 2 cut(s) | 964, 1319 |
| TfiI | GAWTC | 4 cut(s) | 328, 388, 1219, 1345 |
| TscAI | CASTG | 5 cut(s) | 157, 460, 1009, 1594, 1837 |
| TseFI | GTSAC | 3 cut(s) | 636, 653, 1261 |
| TseI | GCWGC | 5 cut(s) | 179, 554, 557, 1133, 1145 |
| Tsp45I | GTSAC | 3 cut(s) | 636, 653, 1261 |
| TspDTI | ATGAA | 6 cut(s) | 828, 950, 1391, 1449, 1659, 1707 |
| TspGWI | ACGGA | 1 cut(s) | 1327 |
| TspRI | CASTG | 5 cut(s) | 157, 460, 1009, 1594, 1837 |
| VspI | ATTAAT | 1 cut(s) | 236 |
| XapI | RAATTY | 4 cut(s) | 917, 937, 1041, 1362 |
| XbaI | TCTAGA | 1 cut(s) | 1037 |
| XceI | RCATGY | 2 cut(s) | 437, 1775 |
| XmaJI | CCTAGG | 1 cut(s) | 649 |
| XmnI | GAANNNNTTC | 1 cut(s) | 941 |
| XspI | CTAG | 2 cut(s) | 650, 1038 |
| ZraI | GACGTC | 1 cut(s) | 670 |