Belongs to the heat shock protein 70 family
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccB7I | CCANNNNNTGG | 1 cut(s) | 663 |
| AccII | CGCG | 1 cut(s) | 529 |
| AciI | CCGC | 4 cut(s) | 5, 229, 443, 630 |
| AclWI | GGATC | 1 cut(s) | 47 |
| AcoI | YGGCCR | 1 cut(s) | 69 |
| AcuI | CTGAAG | 3 cut(s) | 42, 387, 451 |
| AfaI | GTAC | 2 cut(s) | 593, 634 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 663 |
| AgsI | TTSAA | 3 cut(s) | 266, 292, 302 |
| AhlI | ACTAGT | 1 cut(s) | 649 |
| AjnI | CCWGG | 1 cut(s) | 45 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 150, 182 |
| AluBI | AGCT | 3 cut(s) | 20, 461, 503 |
| AluI | AGCT | 3 cut(s) | 20, 461, 503 |
| AlwI | GGATC | 1 cut(s) | 47 |
| AoxI | GGCC | 6 cut(s) | 69, 284, 343, 414, 514, 568 |
| ApeKI | GCWGC | 4 cut(s) | 176, 500, 551, 554 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 202 |
| AspS9I | GGNCC | 2 cut(s) | 305, 343 |
| AsuHPI | GGTGA | 1 cut(s) | 182 |
| AvaII | GGWCC | 1 cut(s) | 305 |
| BauI | CACGAG | 1 cut(s) | 61 |
| BbvCI | CCTCAGC | 1 cut(s) | 411 |
| BbvI | GCAGC | 4 cut(s) | 163, 512, 538, 566 |
| BccI | CCATC | 2 cut(s) | 139, 221 |
| BceAI | ACGGC | 1 cut(s) | 56 |
| BciT130I | CCWGG | 1 cut(s) | 47 |
| BclI | TGATCA | 1 cut(s) | 85 |
| BcuI | ACTAGT | 1 cut(s) | 649 |
| BfaI | CTAG | 1 cut(s) | 650 |
| BfmI | CTRYAG | 1 cut(s) | 552 |
| BisI | GCNGC | 4 cut(s) | 177, 501, 552, 555 |
| BlsI | GCNGC | 4 cut(s) | 178, 502, 553, 556 |
| Bme1390I | CCNGG | 1 cut(s) | 47 |
| Bme18I | GGWCC | 1 cut(s) | 305 |
| BmgT120I | GGNCC | 2 cut(s) | 305, 343 |
| BmrFI | CCNGG | 1 cut(s) | 47 |
| BmsI | GCATC | 4 cut(s) | 142, 163, 211, 387 |
| Bpu10I | CCTNAGC | 1 cut(s) | 411 |
| Bsa29I | ATCGAT | 1 cut(s) | 108 |
| BsaJI | CCNNGG | 2 cut(s) | 46, 421 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 663 |
| Bse118I | RCCGGY | 1 cut(s) | 512 |
| Bse1I | ACTGG | 1 cut(s) | 168 |
| BseBI | CCWGG | 1 cut(s) | 47 |
| BseCI | ATCGAT | 1 cut(s) | 108 |
| BseDI | CCNNGG | 2 cut(s) | 46, 421 |
| BseGI | GGATG | 1 cut(s) | 377 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 663 |
| BseMII | CTCAG | 1 cut(s) | 402 |
| BseNI | ACTGG | 1 cut(s) | 168 |
| BseXI | GCAGC | 4 cut(s) | 163, 512, 538, 566 |
| BseYI | CCCAGC | 1 cut(s) | 457 |
| Bsh1236I | CGCG | 1 cut(s) | 529 |
| BshFI | GGCC | 6 cut(s) | 71, 286, 345, 416, 516, 570 |
| BshVI | ATCGAT | 1 cut(s) | 108 |
| BsiSI | CCGG | 1 cut(s) | 513 |
| BslFI | GGGAC | 2 cut(s) | 64, 440 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 663 |
| BsmFI | GGGAC | 2 cut(s) | 64, 440 |
| BsmI | GAATGC | 1 cut(s) | 445 |
| BsnI | GGCC | 6 cut(s) | 71, 286, 345, 416, 516, 570 |
| Bsp143I | GATC | 4 cut(s) | 39, 85, 105, 109 |
| BspACI | CCGC | 4 cut(s) | 5, 229, 443, 630 |
| BspANI | GGCC | 6 cut(s) | 71, 286, 345, 416, 516, 570 |
| BspCNI | CTCAG | 1 cut(s) | 403 |
| BspDI | ATCGAT | 1 cut(s) | 108 |
| BspFNI | CGCG | 1 cut(s) | 529 |
| BspHI | TCATGA | 1 cut(s) | 310 |
| BspMAI | CTGCAG | 1 cut(s) | 556 |
| BspPI | GGATC | 1 cut(s) | 47 |
| BsrFI | RCCGGY | 1 cut(s) | 512 |
| BsrI | ACTGG | 1 cut(s) | 168 |
| BssAI | RCCGGY | 1 cut(s) | 512 |
| BssECI | CCNNGG | 2 cut(s) | 46, 421 |
| BssMI | GATC | 4 cut(s) | 39, 85, 105, 109 |
| BssSI | CACGAG | 1 cut(s) | 61 |
| Bst2BI | CACGAG | 1 cut(s) | 61 |
| Bst2UI | CCWGG | 1 cut(s) | 47 |
| Bst4CI | ACNGT | 3 cut(s) | 433, 454, 676 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 560 |
| BstC8I | GCNNGC | 2 cut(s) | 514, 586 |
| BstDEI | CTNAG | 1 cut(s) | 411 |
| BstF5I | GGATG | 1 cut(s) | 377 |
| BstFNI | CGCG | 1 cut(s) | 529 |
| BstKTI | GATC | 4 cut(s) | 42, 88, 108, 112 |
| BstMBI | GATC | 4 cut(s) | 39, 85, 105, 109 |
| BstMWI | GCNNNNNNNGC | 3 cut(s) | 509, 560, 594 |
| BstNI | CCWGG | 1 cut(s) | 47 |
| BstNSI | RCATGY | 1 cut(s) | 261 |
| BstSCI | CCNGG | 1 cut(s) | 45 |
| BstSFI | CTRYAG | 1 cut(s) | 552 |
| BstUI | CGCG | 1 cut(s) | 529 |
| BstV1I | GCAGC | 4 cut(s) | 163, 512, 538, 566 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 388 |
| Bsu15I | ATCGAT | 1 cut(s) | 108 |
| BsuRI | GGCC | 6 cut(s) | 71, 286, 345, 416, 516, 570 |
| BsuTUI | ATCGAT | 1 cut(s) | 108 |
| BtsCI | GGATG | 1 cut(s) | 377 |
| BtsIMutI | CAGTG | 3 cut(s) | 147, 475, 514 |
| Cac8I | GCNNGC | 2 cut(s) | 514, 586 |
| CciI | TCATGA | 1 cut(s) | 310 |
| Cfr10I | RCCGGY | 1 cut(s) | 512 |
| Cfr13I | GGNCC | 2 cut(s) | 305, 343 |
| ClaI | ATCGAT | 1 cut(s) | 108 |
| CseI | GACGC | 2 cut(s) | 263, 470 |
| Csp6I | GTAC | 2 cut(s) | 592, 633 |
| CviAII | CATG | 5 cut(s) | 29, 89, 258, 311, 382 |
| CviQI | GTAC | 2 cut(s) | 592, 633 |
| DdeI | CTNAG | 1 cut(s) | 411 |
| DpnI | GATC | 4 cut(s) | 41, 87, 107, 111 |
| DpnII | GATC | 4 cut(s) | 39, 85, 105, 109 |
| EaeI | YGGCCR | 1 cut(s) | 69 |
| Eco147I | AGGCCT | 1 cut(s) | 416 |
| Eco47I | GGWCC | 1 cut(s) | 305 |
| Eco57I | CTGAAG | 3 cut(s) | 42, 387, 451 |
| EcoO109I | RGGNCCY | 1 cut(s) | 305 |
| EcoRII | CCWGG | 1 cut(s) | 45 |
| FaeI | CATG | 5 cut(s) | 32, 92, 261, 314, 385 |
| FaqI | GGGAC | 2 cut(s) | 64, 440 |
| FatI | CATG | 5 cut(s) | 28, 88, 257, 310, 381 |
| FbaI | TGATCA | 1 cut(s) | 85 |
| Fnu4HI | GCNGC | 4 cut(s) | 177, 501, 552, 555 |
| FokI | GGATG | 1 cut(s) | 364 |
| Fsp4HI | GCNGC | 4 cut(s) | 177, 501, 552, 555 |
| FspBI | CTAG | 1 cut(s) | 650 |
| GluI | GCNGC | 4 cut(s) | 177, 501, 552, 555 |
| GsaI | CCCAGC | 1 cut(s) | 461 |
| HaeIII | GGCC | 6 cut(s) | 71, 286, 345, 416, 516, 570 |
| HapII | CCGG | 1 cut(s) | 513 |
| HgaI | GACGC | 2 cut(s) | 263, 470 |
| Hin1II | CATG | 5 cut(s) | 32, 92, 261, 314, 385 |
| HinfI | GANTC | 1 cut(s) | 473 |
| HpaII | CCGG | 1 cut(s) | 513 |
| HphI | GGTGA | 1 cut(s) | 182 |
| Hpy166II | GTNNAC | 1 cut(s) | 679 |
| Hpy188I | TCNGA | 1 cut(s) | 197 |
| Hpy188III | TCNNGA | 3 cut(s) | 37, 266, 311 |
| Hpy8I | GTNNAC | 1 cut(s) | 679 |
| HpyAV | CCTTC | 6 cut(s) | 17, 154, 213, 296, 427, 574 |
| HpyCH4III | ACNGT | 3 cut(s) | 433, 454, 676 |
| HpyCH4IV | ACGT | 1 cut(s) | 138 |
| HpyCH4V | TGCA | 5 cut(s) | 32, 179, 224, 500, 554 |
| HpyF10VI | GCNNNNNNNGC | 3 cut(s) | 509, 560, 594 |
| HpyF3I | CTNAG | 1 cut(s) | 411 |
| HpySE526I | ACGT | 1 cut(s) | 138 |
| Hsp92II | CATG | 5 cut(s) | 32, 92, 261, 314, 385 |
| KroI | GCCGGC | 1 cut(s) | 512 |
| KroNI | GCCGGC | 1 cut(s) | 514 |
| Ksp22I | TGATCA | 1 cut(s) | 85 |
| Kzo9I | GATC | 4 cut(s) | 39, 85, 105, 109 |
| Lsp1109I | GCAGC | 4 cut(s) | 163, 512, 538, 566 |
| LweI | GCATC | 4 cut(s) | 142, 163, 211, 387 |
| MaeI | CTAG | 1 cut(s) | 650 |
| MaeII | ACGT | 1 cut(s) | 138 |
| MaeIII | GTNAC | 6 cut(s) | 116, 251, 331, 470, 645, 652 |
| MalI | GATC | 4 cut(s) | 41, 87, 107, 111 |
| MboI | GATC | 4 cut(s) | 39, 85, 105, 109 |
| MboII | GAAGA | 2 cut(s) | 255, 380 |
| MluCI | AATT | 1 cut(s) | 234 |
| MlyI | GAGTC | 1 cut(s) | 467 |
| MmeI | TCCRAC | 1 cut(s) | 231 |
| MnlI | CCTC | 3 cut(s) | 318, 406, 431 |
| MroNI | GCCGGC | 1 cut(s) | 512 |
| MroXI | GAANNNNTTC | 1 cut(s) | 202 |
| MseI | TTAA | 4 cut(s) | 183, 233, 276, 435 |
| MspI | CCGG | 1 cut(s) | 513 |
| MspR9I | CCNGG | 1 cut(s) | 47 |
| Mva1269I | GAATGC | 1 cut(s) | 445 |
| MvaI | CCWGG | 1 cut(s) | 47 |
| MvnI | CGCG | 1 cut(s) | 529 |
| MwoI | GCNNNNNNNGC | 3 cut(s) | 509, 560, 594 |
| NaeI | GCCGGC | 1 cut(s) | 514 |
| NdeII | GATC | 4 cut(s) | 39, 85, 105, 109 |
| NgoMIV | GCCGGC | 1 cut(s) | 512 |
| NlaIII | CATG | 5 cut(s) | 32, 92, 261, 314, 385 |
| NmeAIII | GCCGAG | 1 cut(s) | 402 |
| NmuCI | GTSAC | 4 cut(s) | 251, 331, 470, 645 |
| NspI | RCATGY | 1 cut(s) | 261 |
| PagI | TCATGA | 1 cut(s) | 310 |
| PceI | AGGCCT | 1 cut(s) | 416 |
| PctI | GAATGC | 1 cut(s) | 445 |
| PdiI | GCCGGC | 1 cut(s) | 514 |
| PdmI | GAANNNNTTC | 1 cut(s) | 202 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 663 |
| PkrI | GCNGC | 4 cut(s) | 178, 502, 553, 556 |
| PleI | GAGTC | 1 cut(s) | 467 |
| PpsI | GAGTC | 1 cut(s) | 467 |
| PpuMI | RGGWCCY | 1 cut(s) | 305 |
| Psp5II | RGGWCCY | 1 cut(s) | 305 |
| Psp6I | CCWGG | 1 cut(s) | 45 |
| PspFI | CCCAGC | 1 cut(s) | 457 |
| PspGI | CCWGG | 1 cut(s) | 45 |
| PspPI | GGNCC | 2 cut(s) | 305, 343 |
| PspPPI | RGGWCCY | 1 cut(s) | 305 |
| PstI | CTGCAG | 1 cut(s) | 556 |
| RsaI | GTAC | 2 cut(s) | 593, 634 |
| RsaNI | GTAC | 2 cut(s) | 592, 633 |
| SaqAI | TTAA | 4 cut(s) | 183, 233, 276, 435 |
| SatI | GCNGC | 4 cut(s) | 177, 501, 552, 555 |
| Sau3AI | GATC | 4 cut(s) | 39, 85, 105, 109 |
| Sau96I | GGNCC | 2 cut(s) | 305, 343 |
| SchI | GAGTC | 1 cut(s) | 467 |
| ScrFI | CCNGG | 1 cut(s) | 47 |
| SfaNI | GCATC | 4 cut(s) | 142, 163, 211, 387 |
| SfcI | CTRYAG | 1 cut(s) | 552 |
| SinI | GGWCC | 1 cut(s) | 305 |
| SpeI | ACTAGT | 1 cut(s) | 649 |
| Sse9I | AATT | 1 cut(s) | 234 |
| SseBI | AGGCCT | 1 cut(s) | 416 |
| SsiI | CCGC | 4 cut(s) | 5, 229, 443, 630 |
| SspMI | CTAG | 1 cut(s) | 650 |
| StuI | AGGCCT | 1 cut(s) | 416 |
| StyD4I | CCNGG | 1 cut(s) | 45 |
| TaaI | ACNGT | 3 cut(s) | 433, 454, 676 |
| TaiI | ACGT | 1 cut(s) | 141 |
| TaqI | TCGA | 2 cut(s) | 42, 108 |
| TasI | AATT | 1 cut(s) | 234 |
| Tru1I | TTAA | 4 cut(s) | 183, 233, 276, 435 |
| Tru9I | TTAA | 4 cut(s) | 183, 233, 276, 435 |
| TscAI | CASTG | 3 cut(s) | 154, 475, 514 |
| TseFI | GTSAC | 4 cut(s) | 251, 331, 470, 645 |
| TseI | GCWGC | 4 cut(s) | 176, 500, 551, 554 |
| Tsp45I | GTSAC | 4 cut(s) | 251, 331, 470, 645 |
| TspDTI | ATGAA | 1 cut(s) | 555 |
| TspRI | CASTG | 3 cut(s) | 154, 475, 514 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 663 |
| VpaK11BI | GGWCC | 1 cut(s) | 305 |
| XceI | RCATGY | 1 cut(s) | 261 |
| XmnI | GAANNNNTTC | 1 cut(s) | 202 |
| XspI | CTAG | 1 cut(s) | 650 |