Belongs to the heat shock protein 70 family
Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AatII | GACGTC | 1 cut(s) | 417 |
| AccB1I | GGYRCC | 1 cut(s) | 773 |
| AccI | GTMKAC | 1 cut(s) | 491 |
| AciI | CCGC | 2 cut(s) | 777, 1092 |
| AclWI | GGATC | 1 cut(s) | 160 |
| AcoI | YGGCCR | 2 cut(s) | 11, 1171 |
| AcsI | RAATTY | 2 cut(s) | 560, 942 |
| AcuI | CTGAAG | 2 cut(s) | 114, 159 |
| AcyI | GRCGYC | 1 cut(s) | 414 |
| AdeI | CACNNNGTG | 1 cut(s) | 773 |
| AfaI | GTAC | 1 cut(s) | 813 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 211, 757 |
| AflII | CTTAAG | 1 cut(s) | 1061 |
| AflIII | ACRYGT | 1 cut(s) | 1161 |
| AgeI | ACCGGT | 1 cut(s) | 668 |
| AgsI | TTSAA | 7 cut(s) | 19, 196, 394, 506, 566, 799, 827 |
| AjnI | CCWGG | 1 cut(s) | 814 |
| AjuI | GAANNNNNNNTTGG | 4 cut(s) | 132, 164, 392, 424 |
| AluBI | AGCT | 8 cut(s) | 7, 143, 239, 733, 1002, 1066, 1085, 1154 |
| AluI | AGCT | 8 cut(s) | 7, 143, 239, 733, 1002, 1066, 1085, 1154 |
| AlwI | GGATC | 1 cut(s) | 160 |
| Ama87I | CYCGRG | 1 cut(s) | 937 |
| AoxI | GGCC | 4 cut(s) | 11, 241, 295, 1171 |
| ApeKI | GCWGC | 4 cut(s) | 89, 278, 281, 788 |
| ApoI | RAATTY | 2 cut(s) | 560, 942 |
| AsiGI | ACCGGT | 1 cut(s) | 668 |
| Asp700I | GAANNNNTTC | 2 cut(s) | 478, 716 |
| AspS9I | GGNCC | 1 cut(s) | 32 |
| AsuHPI | GGTGA | 4 cut(s) | 262, 359, 841, 1031 |
| AvaI | CYCGRG | 1 cut(s) | 937 |
| AvaII | GGWCC | 1 cut(s) | 32 |
| AxyI | CCTNAGG | 1 cut(s) | 950 |
| BalI | TGGCCA | 2 cut(s) | 13, 1173 |
| BanI | GGYRCC | 1 cut(s) | 773 |
| BbsI | GAAGAC | 3 cut(s) | 357, 481, 669 |
| BbvCI | CCTCAGC | 1 cut(s) | 1155 |
| BbvI | GCAGC | 4 cut(s) | 76, 265, 293, 775 |
| BccI | CCATC | 2 cut(s) | 887, 956 |
| BceAI | ACGGC | 1 cut(s) | 892 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 257, 291 |
| BciT130I | CCWGG | 1 cut(s) | 816 |
| BciVI | GTATCC | 1 cut(s) | 1148 |
| BfaI | CTAG | 1 cut(s) | 36 |
| BfmI | CTRYAG | 3 cut(s) | 279, 762, 789 |
| BfrI | CTTAAG | 1 cut(s) | 1061 |
| BfuI | GTATCC | 1 cut(s) | 1148 |
| BisI | GCNGC | 5 cut(s) | 90, 279, 282, 777, 789 |
| BlsI | GCNGC | 5 cut(s) | 91, 280, 283, 778, 790 |
| Bme1390I | CCNGG | 1 cut(s) | 816 |
| Bme18I | GGWCC | 1 cut(s) | 32 |
| BmeT110I | CYCGRG | 1 cut(s) | 937 |
| BmgT120I | GGNCC | 1 cut(s) | 32 |
| BmiI | GGNNCC | 3 cut(s) | 603, 775, 906 |
| BmrFI | CCNGG | 1 cut(s) | 816 |
| BmsI | GCATC | 5 cut(s) | 114, 243, 529, 616, 1117 |
| BpiI | GAAGAC | 3 cut(s) | 357, 481, 669 |
| BpmI | CTGGAG | 2 cut(s) | 348, 1065 |
| Bpu10I | CCTNAGC | 1 cut(s) | 1155 |
| BpuEI | CTTGAG | 1 cut(s) | 943 |
| BsaAI | YACGTR | 1 cut(s) | 489 |
| BsaBI | GATNNNNATC | 1 cut(s) | 1190 |
| BsaHI | GRCGYC | 1 cut(s) | 414 |
| BsaJI | CCNNGG | 4 cut(s) | 148, 340, 690, 815 |
| BsaWI | WCCGGW | 1 cut(s) | 668 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 211, 757 |
| Bse118I | RCCGGY | 1 cut(s) | 668 |
| Bse1I | ACTGG | 3 cut(s) | 1174, 1212, 1229 |
| Bse21I | CCTNAGG | 1 cut(s) | 950 |
| Bse3DI | GCAATG | 2 cut(s) | 285, 1074 |
| Bse8I | GATNNNNATC | 1 cut(s) | 1190 |
| BseBI | CCWGG | 1 cut(s) | 816 |
| BseDI | CCNNGG | 4 cut(s) | 148, 340, 690, 815 |
| BseGI | GGATG | 3 cut(s) | 631, 658, 850 |
| BseJI | GATNNNNATC | 1 cut(s) | 1190 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 211, 757 |
| BseMI | GCAATG | 2 cut(s) | 285, 1074 |
| BseMII | CTCAG | 3 cut(s) | 941, 1133, 1146 |
| BseNI | ACTGG | 3 cut(s) | 1174, 1212, 1229 |
| BseXI | GCAGC | 4 cut(s) | 76, 265, 293, 775 |
| BseYI | CCCAGC | 1 cut(s) | 1088 |
| BshFI | GGCC | 4 cut(s) | 13, 243, 297, 1173 |
| BshNI | GGYRCC | 1 cut(s) | 773 |
| BshTI | ACCGGT | 1 cut(s) | 668 |
| BsiHKCI | CYCGRG | 1 cut(s) | 937 |
| BsiSI | CCGG | 1 cut(s) | 669 |
| BslFI | GGGAC | 1 cut(s) | 167 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 211, 757 |
| BsmFI | GGGAC | 1 cut(s) | 167 |
| BsnI | GGCC | 4 cut(s) | 13, 243, 297, 1173 |
| BsoBI | CYCGRG | 1 cut(s) | 937 |
| Bsp143I | GATC | 2 cut(s) | 152, 1191 |
| BspACI | CCGC | 2 cut(s) | 777, 1092 |
| BspANI | GGCC | 4 cut(s) | 13, 243, 297, 1173 |
| BspCNI | CTCAG | 3 cut(s) | 942, 1134, 1147 |
| BspHI | TCATGA | 1 cut(s) | 912 |
| BspLI | GGNNCC | 3 cut(s) | 603, 775, 906 |
| BspMAI | CTGCAG | 2 cut(s) | 283, 793 |
| BspPI | GGATC | 1 cut(s) | 160 |
| BspT107I | GGYRCC | 1 cut(s) | 773 |
| BspTI | CTTAAG | 1 cut(s) | 1061 |
| BsrDI | GCAATG | 2 cut(s) | 285, 1074 |
| BsrFI | RCCGGY | 1 cut(s) | 668 |
| BsrI | ACTGG | 3 cut(s) | 1174, 1212, 1229 |
| BssAI | RCCGGY | 1 cut(s) | 668 |
| BssECI | CCNNGG | 4 cut(s) | 148, 340, 690, 815 |
| BssMI | GATC | 2 cut(s) | 152, 1191 |
| BssNAI | GTATAC | 1 cut(s) | 492 |
| BssNI | GRCGYC | 1 cut(s) | 414 |
| BssT1I | CCWWGG | 3 cut(s) | 148, 340, 690 |
| Bst1107I | GTATAC | 1 cut(s) | 492 |
| Bst2UI | CCWGG | 1 cut(s) | 816 |
| Bst4CI | ACNGT | 4 cut(s) | 160, 181, 976, 1199 |
| Bst6I | CTCTTC | 1 cut(s) | 950 |
| BstACI | GRCGYC | 1 cut(s) | 414 |
| BstAFI | CTTAAG | 1 cut(s) | 1061 |
| BstAPI | GCANNNNNTGC | 2 cut(s) | 287, 773 |
| BstBAI | YACGTR | 1 cut(s) | 489 |
| BstC8I | GCNNGC | 2 cut(s) | 229, 241 |
| BstDEI | CTNAG | 4 cut(s) | 437, 950, 1142, 1155 |
| BstEII | GGTNACC | 1 cut(s) | 58 |
| BstF5I | GGATG | 3 cut(s) | 631, 658, 850 |
| BstKTI | GATC | 2 cut(s) | 155, 1194 |
| BstMBI | GATC | 2 cut(s) | 152, 1191 |
| BstMWI | GCNNNNNNNGC | 4 cut(s) | 287, 317, 773, 785 |
| BstNI | CCWGG | 1 cut(s) | 816 |
| BstNSI | RCATGY | 2 cut(s) | 227, 1165 |
| BstPI | GGTNACC | 1 cut(s) | 58 |
| BstSCI | CCNGG | 1 cut(s) | 814 |
| BstSFI | CTRYAG | 3 cut(s) | 279, 762, 789 |
| BstSNI | TACGTA | 1 cut(s) | 489 |
| BstV1I | GCAGC | 4 cut(s) | 76, 265, 293, 775 |
| BstV2I | GAAGAC | 3 cut(s) | 357, 481, 669 |
| BstZ17I | GTATAC | 1 cut(s) | 492 |
| Bsu36I | CCTNAGG | 1 cut(s) | 950 |
| BsuI | GTATCC | 1 cut(s) | 1148 |
| BsuRI | GGCC | 4 cut(s) | 13, 243, 297, 1173 |
| BtsCI | GGATG | 3 cut(s) | 631, 658, 850 |
| BtsI | GCAGTG | 3 cut(s) | 318, 321, 652 |
| BtsIMutI | CAGTG | 8 cut(s) | 177, 318, 321, 424, 652, 1167, 1204, 1219 |
| Cac8I | GCNNGC | 2 cut(s) | 229, 241 |
| CciI | TCATGA | 1 cut(s) | 912 |
| Cfr10I | RCCGGY | 1 cut(s) | 668 |
| Cfr13I | GGNCC | 1 cut(s) | 32 |
| CseI | GACGC | 1 cut(s) | 370 |
| Csp6I | GTAC | 1 cut(s) | 812 |
| CspAI | ACCGGT | 1 cut(s) | 668 |
| CviAII | CATG | 6 cut(s) | 224, 404, 721, 770, 913, 1162 |
| CviQI | GTAC | 1 cut(s) | 812 |
| DdeI | CTNAG | 4 cut(s) | 437, 950, 1142, 1155 |
| DpnI | GATC | 2 cut(s) | 154, 1193 |
| DpnII | GATC | 2 cut(s) | 152, 1191 |
| DraIII | CACNNNGTG | 1 cut(s) | 773 |
| EaeI | YGGCCR | 2 cut(s) | 11, 1171 |
| Eam1104I | CTCTTC | 1 cut(s) | 950 |
| EarI | CTCTTC | 1 cut(s) | 950 |
| Eco105I | TACGTA | 1 cut(s) | 489 |
| Eco130I | CCWWGG | 3 cut(s) | 148, 340, 690 |
| Eco47I | GGWCC | 1 cut(s) | 32 |
| Eco57I | CTGAAG | 2 cut(s) | 114, 159 |
| Eco81I | CCTNAGG | 1 cut(s) | 950 |
| Eco88I | CYCGRG | 1 cut(s) | 937 |
| Eco91I | GGTNACC | 1 cut(s) | 58 |
| EcoO109I | RGGNCCY | 1 cut(s) | 32 |
| EcoO65I | GGTNACC | 1 cut(s) | 58 |
| EcoRI | GAATTC | 1 cut(s) | 942 |
| EcoRII | CCWGG | 1 cut(s) | 814 |
| EcoT14I | CCWWGG | 3 cut(s) | 148, 340, 690 |
| ErhI | CCWWGG | 3 cut(s) | 148, 340, 690 |
| FaeI | CATG | 6 cut(s) | 227, 407, 724, 773, 916, 1165 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 132, 164 |
| FaqI | GGGAC | 1 cut(s) | 167 |
| FatI | CATG | 6 cut(s) | 223, 403, 720, 769, 912, 1161 |
| FblI | GTMKAC | 1 cut(s) | 491 |
| Fnu4HI | GCNGC | 5 cut(s) | 90, 279, 282, 777, 789 |
| FokI | GGATG | 3 cut(s) | 638, 665, 857 |
| Fsp4HI | GCNGC | 5 cut(s) | 90, 279, 282, 777, 789 |
| FspBI | CTAG | 1 cut(s) | 36 |
| GluI | GCNGC | 5 cut(s) | 90, 279, 282, 777, 789 |
| GsaI | CCCAGC | 1 cut(s) | 1092 |
| GsuI | CTGGAG | 2 cut(s) | 348, 1065 |
| HaeIII | GGCC | 4 cut(s) | 13, 243, 297, 1173 |
| HapII | CCGG | 1 cut(s) | 669 |
| HgaI | GACGC | 1 cut(s) | 370 |
| Hin1I | GRCGYC | 1 cut(s) | 414 |
| Hin1II | CATG | 6 cut(s) | 227, 407, 724, 773, 916, 1165 |
| HincII | GTYRAC | 1 cut(s) | 373 |
| HindII | GTYRAC | 1 cut(s) | 373 |
| HindIII | AAGCTT | 2 cut(s) | 141, 1064 |
| HinfI | GANTC | 3 cut(s) | 684, 925, 1104 |
| HpaI | GTTAAC | 1 cut(s) | 373 |
| HpaII | CCGG | 1 cut(s) | 669 |
| HphI | GGTGA | 4 cut(s) | 262, 359, 841, 1031 |
| Hpy166II | GTNNAC | 4 cut(s) | 373, 486, 492, 1167 |
| Hpy188I | TCNGA | 1 cut(s) | 51 |
| Hpy188III | TCNNGA | 7 cut(s) | 394, 681, 827, 913, 922, 939, 1141 |
| Hpy8I | GTNNAC | 4 cut(s) | 373, 486, 492, 1167 |
| Hpy99I | CGWCG | 1 cut(s) | 386 |
| HpyAV | CCTTC | 2 cut(s) | 721, 1211 |
| HpyCH4III | ACNGT | 4 cut(s) | 160, 181, 976, 1199 |
| HpyCH4IV | ACGT | 3 cut(s) | 208, 414, 488 |
| HpyCH4V | TGCA | 6 cut(s) | 119, 281, 520, 697, 738, 791 |
| HpyF10VI | GCNNNNNNNGC | 4 cut(s) | 287, 317, 773, 785 |
| HpyF3I | CTNAG | 4 cut(s) | 437, 950, 1142, 1155 |
| HpySE526I | ACGT | 3 cut(s) | 208, 414, 488 |
| Hsp92I | GRCGYC | 1 cut(s) | 414 |
| Hsp92II | CATG | 6 cut(s) | 227, 407, 724, 773, 916, 1165 |
| KspAI | GTTAAC | 1 cut(s) | 373 |
| Kzo9I | GATC | 2 cut(s) | 152, 1191 |
| LmnI | GCTCC | 5 cut(s) | 601, 730, 904, 1084, 1090 |
| Lsp1109I | GCAGC | 4 cut(s) | 76, 265, 293, 775 |
| LweI | GCATC | 5 cut(s) | 114, 243, 529, 616, 1117 |
| MaeI | CTAG | 1 cut(s) | 36 |
| MaeII | ACGT | 3 cut(s) | 208, 414, 488 |
| MaeIII | GTNAC | 6 cut(s) | 58, 541, 847, 858, 967, 1199 |
| MalI | GATC | 2 cut(s) | 154, 1193 |
| MboI | GATC | 2 cut(s) | 152, 1191 |
| MboII | GAAGA | 7 cut(s) | 107, 362, 486, 577, 669, 967, 1218 |
| MlsI | TGGCCA | 2 cut(s) | 13, 1173 |
| MluCI | AATT | 8 cut(s) | 24, 132, 389, 507, 560, 609, 942, 1221 |
| MluNI | TGGCCA | 2 cut(s) | 13, 1173 |
| MlyI | GAGTC | 1 cut(s) | 934 |
| Mox20I | TGGCCA | 2 cut(s) | 13, 1173 |
| MroXI | GAANNNNTTC | 2 cut(s) | 478, 716 |
| MscI | TGGCCA | 2 cut(s) | 13, 1173 |
| MseI | TTAA | 6 cut(s) | 264, 372, 446, 591, 612, 1062 |
| Msp20I | TGGCCA | 2 cut(s) | 13, 1173 |
| MspA1I | CMGCKG | 3 cut(s) | 779, 1092, 1154 |
| MspCI | CTTAAG | 1 cut(s) | 1061 |
| MspI | CCGG | 1 cut(s) | 669 |
| MspR9I | CCNGG | 1 cut(s) | 816 |
| MvaI | CCWGG | 1 cut(s) | 816 |
| MwoI | GCNNNNNNNGC | 4 cut(s) | 287, 317, 773, 785 |
| NdeII | GATC | 2 cut(s) | 152, 1191 |
| NlaIII | CATG | 6 cut(s) | 227, 407, 724, 773, 916, 1165 |
| NlaIV | GGNNCC | 3 cut(s) | 603, 775, 906 |
| NmuCI | GTSAC | 3 cut(s) | 847, 858, 1199 |
| NspI | RCATGY | 2 cut(s) | 227, 1165 |
| PaeR7I | CTCGAG | 1 cut(s) | 937 |
| PagI | TCATGA | 1 cut(s) | 912 |
| PciI | ACATGT | 1 cut(s) | 1161 |
| PdmI | GAANNNNTTC | 2 cut(s) | 478, 716 |
| PfeI | GAWTC | 2 cut(s) | 684, 1104 |
| PinAI | ACCGGT | 1 cut(s) | 668 |
| PkrI | GCNGC | 5 cut(s) | 91, 280, 283, 778, 790 |
| PleI | GAGTC | 1 cut(s) | 933 |
| PpsI | GAGTC | 1 cut(s) | 933 |
| Ppu21I | YACGTR | 1 cut(s) | 489 |
| PpuMI | RGGWCCY | 1 cut(s) | 32 |
| PscI | ACATGT | 1 cut(s) | 1161 |
| Psp5II | RGGWCCY | 1 cut(s) | 32 |
| Psp6I | CCWGG | 1 cut(s) | 814 |
| PspEI | GGTNACC | 1 cut(s) | 58 |
| PspFI | CCCAGC | 1 cut(s) | 1088 |
| PspGI | CCWGG | 1 cut(s) | 814 |
| PspN4I | GGNNCC | 3 cut(s) | 603, 775, 906 |
| PspPI | GGNCC | 1 cut(s) | 32 |
| PspPPI | RGGWCCY | 1 cut(s) | 32 |
| PstI | CTGCAG | 2 cut(s) | 283, 793 |
| PvuII | CAGCTG | 1 cut(s) | 1154 |
| RsaI | GTAC | 1 cut(s) | 813 |
| RsaNI | GTAC | 1 cut(s) | 812 |
| SaqAI | TTAA | 6 cut(s) | 264, 372, 446, 591, 612, 1062 |
| SatI | GCNGC | 5 cut(s) | 90, 279, 282, 777, 789 |
| Sau3AI | GATC | 2 cut(s) | 152, 1191 |
| Sau96I | GGNCC | 1 cut(s) | 32 |
| SchI | GAGTC | 1 cut(s) | 934 |
| ScrFI | CCNGG | 1 cut(s) | 816 |
| SfaNI | GCATC | 5 cut(s) | 114, 243, 529, 616, 1117 |
| SfcI | CTRYAG | 3 cut(s) | 279, 762, 789 |
| Sfr274I | CTCGAG | 1 cut(s) | 937 |
| SinI | GGWCC | 1 cut(s) | 32 |
| SlaI | CTCGAG | 1 cut(s) | 937 |
| SmlI | CTYRAG | 3 cut(s) | 922, 937, 1061 |
| SmoI | CTYRAG | 3 cut(s) | 922, 937, 1061 |
| SnaBI | TACGTA | 1 cut(s) | 489 |
| Sse9I | AATT | 8 cut(s) | 24, 132, 389, 507, 560, 609, 942, 1221 |
| SsiI | CCGC | 2 cut(s) | 777, 1092 |
| SspI | AATATT | 1 cut(s) | 83 |
| SspMI | CTAG | 1 cut(s) | 36 |
| StyD4I | CCNGG | 1 cut(s) | 814 |
| StyI | CCWWGG | 3 cut(s) | 148, 340, 690 |
| TaaI | ACNGT | 4 cut(s) | 160, 181, 976, 1199 |
| TaiI | ACGT | 3 cut(s) | 211, 417, 491 |
| TaqI | TCGA | 4 cut(s) | 357, 384, 682, 938 |
| TasI | AATT | 8 cut(s) | 24, 132, 389, 507, 560, 609, 942, 1221 |
| TauI | GCSGC | 1 cut(s) | 779 |
| TfiI | GAWTC | 2 cut(s) | 684, 1104 |
| Tru1I | TTAA | 6 cut(s) | 264, 372, 446, 591, 612, 1062 |
| Tru9I | TTAA | 6 cut(s) | 264, 372, 446, 591, 612, 1062 |
| TscAI | CASTG | 8 cut(s) | 184, 318, 328, 424, 652, 1174, 1204, 1219 |
| TseFI | GTSAC | 3 cut(s) | 847, 858, 1199 |
| TseI | GCWGC | 4 cut(s) | 89, 278, 281, 788 |
| Tsp45I | GTSAC | 3 cut(s) | 847, 858, 1199 |
| TspDTI | ATGAA | 4 cut(s) | 17, 709, 901, 929 |
| TspRI | CASTG | 8 cut(s) | 184, 318, 328, 424, 652, 1174, 1204, 1219 |
| Vha464I | CTTAAG | 1 cut(s) | 1061 |
| VpaK11BI | GGWCC | 1 cut(s) | 32 |
| XapI | RAATTY | 2 cut(s) | 560, 942 |
| XceI | RCATGY | 2 cut(s) | 227, 1165 |
| XhoI | CTCGAG | 1 cut(s) | 937 |
| XmiI | GTMKAC | 1 cut(s) | 491 |
| XmnI | GAANNNNTTC | 2 cut(s) | 478, 716 |
| XspI | CTAG | 1 cut(s) | 36 |
| ZraI | GACGTC | 1 cut(s) | 415 |