Belongs to the heat shock protein 70 family
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccBSI | CCGCTC | 1 cut(s) | 1167 |
| AccII | CGCG | 2 cut(s) | 1142, 1706 |
| AciI | CCGC | 5 cut(s) | 552, 1142, 1167, 1628, 1706 |
| AclWI | GGATC | 3 cut(s) | 954, 1048, 1800 |
| AcoI | YGGCCR | 1 cut(s) | 1143 |
| AcsI | RAATTY | 6 cut(s) | 245, 669, 926, 1050, 1473, 1613 |
| AcuI | CTGAAG | 1 cut(s) | 243 |
| AfaI | GTAC | 5 cut(s) | 297, 634, 750, 1062, 1595 |
| AfiI | CCNNNNNNNGG | 5 cut(s) | 35, 156, 708, 709, 1842 |
| AflII | CTTAAG | 1 cut(s) | 392 |
| AgsI | TTSAA | 5 cut(s) | 292, 381, 662, 852, 1187 |
| AjnI | CCWGG | 5 cut(s) | 186, 307, 942, 1490, 1841 |
| AluBI | AGCT | 8 cut(s) | 32, 280, 937, 1099, 1154, 1370, 1421, 1702 |
| AluI | AGCT | 8 cut(s) | 32, 280, 937, 1099, 1154, 1370, 1421, 1702 |
| Alw21I | GWGCWC | 2 cut(s) | 939, 1704 |
| Alw26I | GTCTC | 3 cut(s) | 832, 1489, 1530 |
| AlwI | GGATC | 3 cut(s) | 954, 1048, 1800 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 554 |
| Ama87I | CYCGRG | 1 cut(s) | 1697 |
| AoxI | GGCC | 4 cut(s) | 284, 414, 1143, 1408 |
| ApeKI | GCWGC | 5 cut(s) | 11, 176, 554, 1421, 1510 |
| ApoI | RAATTY | 6 cut(s) | 245, 669, 926, 1050, 1473, 1613 |
| AspLEI | GCGC | 1 cut(s) | 1142 |
| AspS9I | GGNCC | 2 cut(s) | 26, 305 |
| AsuC2I | CCSGG | 1 cut(s) | 157 |
| AsuHPI | GGTGA | 5 cut(s) | 32, 182, 691, 1367, 1859 |
| AvaI | CYCGRG | 1 cut(s) | 1697 |
| AvaII | GGWCC | 2 cut(s) | 26, 305 |
| AxyI | CCTNAGG | 1 cut(s) | 366 |
| BanII | GRGCYC | 2 cut(s) | 939, 1704 |
| Bbv12I | GWGCWC | 2 cut(s) | 939, 1704 |
| BbvI | GCAGC | 4 cut(s) | 163, 541, 1408, 1522 |
| BccI | CCATC | 6 cut(s) | 212, 875, 884, 1567, 1773, 1844 |
| BceAI | ACGGC | 1 cut(s) | 1820 |
| BcgI | CGANNNNNNTGC | 4 cut(s) | 68, 102, 1445, 1479 |
| BciT130I | CCWGG | 5 cut(s) | 188, 309, 944, 1492, 1843 |
| BclI | TGATCA | 1 cut(s) | 109 |
| BcnI | CCSGG | 1 cut(s) | 157 |
| BcoDI | GTCTC | 3 cut(s) | 832, 1489, 1530 |
| BfaI | CTAG | 4 cut(s) | 623, 1047, 1418, 1808 |
| BfmI | CTRYAG | 1 cut(s) | 546 |
| BfrI | CTTAAG | 1 cut(s) | 392 |
| BisI | GCNGC | 7 cut(s) | 12, 177, 552, 555, 1143, 1422, 1511 |
| BlsI | GCNGC | 7 cut(s) | 13, 178, 553, 556, 1144, 1423, 1512 |
| Bme1390I | CCNGG | 6 cut(s) | 157, 188, 309, 944, 1492, 1843 |
| Bme18I | GGWCC | 2 cut(s) | 26, 305 |
| BmeT110I | CYCGRG | 1 cut(s) | 1697 |
| BmgT120I | GGNCC | 2 cut(s) | 26, 305 |
| BmiI | GGNNCC | 2 cut(s) | 27, 1171 |
| BmrFI | CCNGG | 6 cut(s) | 157, 188, 309, 944, 1492, 1843 |
| BmsI | GCATC | 8 cut(s) | 163, 211, 244, 387, 487, 982, 1119, 1453 |
| BpmI | CTGGAG | 1 cut(s) | 714 |
| BpuMI | CCSGG | 1 cut(s) | 157 |
| Bsa29I | ATCGAT | 2 cut(s) | 618, 876 |
| BsaBI | GATNNNNATC | 1 cut(s) | 1526 |
| BsaJI | CCNNGG | 7 cut(s) | 46, 308, 421, 706, 1222, 1491, 1842 |
| Bsc4I | CCNNNNNNNGG | 5 cut(s) | 35, 156, 708, 709, 1842 |
| Bse118I | RCCGGY | 1 cut(s) | 512 |
| Bse21I | CCTNAGG | 1 cut(s) | 366 |
| Bse3DI | GCAATG | 1 cut(s) | 99 |
| Bse8I | GATNNNNATC | 1 cut(s) | 1526 |
| BseBI | CCWGG | 5 cut(s) | 188, 309, 944, 1492, 1843 |
| BseCI | ATCGAT | 2 cut(s) | 618, 876 |
| BseDI | CCNNGG | 7 cut(s) | 46, 308, 421, 706, 1222, 1491, 1842 |
| BseGI | GGATG | 5 cut(s) | 502, 997, 1210, 1260, 1612 |
| BseJI | GATNNNNATC | 1 cut(s) | 1526 |
| BseLI | CCNNNNNNNGG | 5 cut(s) | 35, 156, 708, 709, 1842 |
| BseMI | GCAATG | 1 cut(s) | 99 |
| BseMII | CTCAG | 3 cut(s) | 357, 1419, 1764 |
| BseRI | GAGGAG | 3 cut(s) | 383, 947, 1203 |
| BseXI | GCAGC | 4 cut(s) | 163, 541, 1408, 1522 |
| Bsh1236I | CGCG | 2 cut(s) | 1142, 1706 |
| BshFI | GGCC | 4 cut(s) | 286, 416, 1145, 1410 |
| BshVI | ATCGAT | 2 cut(s) | 618, 876 |
| BsiHKAI | GWGCWC | 2 cut(s) | 939, 1704 |
| BsiHKCI | CYCGRG | 1 cut(s) | 1697 |
| BsiSI | CCGG | 2 cut(s) | 156, 513 |
| BslFI | GGGAC | 2 cut(s) | 631, 1508 |
| BslI | CCNNNNNNNGG | 5 cut(s) | 35, 156, 708, 709, 1842 |
| BsmAI | GTCTC | 3 cut(s) | 832, 1489, 1530 |
| BsmFI | GGGAC | 2 cut(s) | 631, 1508 |
| BsmI | GAATGC | 1 cut(s) | 1732 |
| BsnI | GGCC | 4 cut(s) | 286, 416, 1145, 1410 |
| BsoBI | CYCGRG | 1 cut(s) | 1697 |
| Bsp1286I | GDGCHC | 2 cut(s) | 939, 1704 |
| Bsp1407I | TGTACA | 1 cut(s) | 748 |
| BspACI | CCGC | 5 cut(s) | 552, 1142, 1167, 1628, 1706 |
| BspANI | GGCC | 4 cut(s) | 286, 416, 1145, 1410 |
| BspCNI | CTCAG | 3 cut(s) | 358, 1418, 1765 |
| BspDI | ATCGAT | 2 cut(s) | 618, 876 |
| BspFNI | CGCG | 2 cut(s) | 1142, 1706 |
| BspLI | GGNNCC | 2 cut(s) | 27, 1171 |
| BspPI | GGATC | 3 cut(s) | 954, 1048, 1800 |
| BspTI | CTTAAG | 1 cut(s) | 392 |
| BsrBI | CCGCTC | 1 cut(s) | 1167 |
| BsrDI | GCAATG | 1 cut(s) | 99 |
| BsrFI | RCCGGY | 1 cut(s) | 512 |
| BsrGI | TGTACA | 1 cut(s) | 748 |
| BssAI | RCCGGY | 1 cut(s) | 512 |
| BssECI | CCNNGG | 7 cut(s) | 46, 308, 421, 706, 1222, 1491, 1842 |
| BssT1I | CCWWGG | 3 cut(s) | 46, 706, 1222 |
| Bst2UI | CCWGG | 5 cut(s) | 188, 309, 944, 1492, 1843 |
| Bst4CI | ACNGT | 7 cut(s) | 132, 454, 971, 1091, 1312, 1678, 1749 |
| Bst6I | CTCTTC | 1 cut(s) | 657 |
| BstAFI | CTTAAG | 1 cut(s) | 392 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 11 |
| BstAUI | TGTACA | 1 cut(s) | 748 |
| BstC8I | GCNNGC | 2 cut(s) | 514, 1704 |
| BstDEI | CTNAG | 4 cut(s) | 366, 1341, 1405, 1773 |
| BstF5I | GGATG | 5 cut(s) | 502, 997, 1210, 1260, 1612 |
| BstFNI | CGCG | 2 cut(s) | 1142, 1706 |
| BstHHI | GCGC | 1 cut(s) | 1142 |
| BstMAI | GTCTC | 3 cut(s) | 832, 1489, 1530 |
| BstMWI | GCNNNNNNNGC | 5 cut(s) | 11, 560, 1139, 1151, 1640 |
| BstNI | CCWGG | 5 cut(s) | 188, 309, 944, 1492, 1843 |
| BstSCI | CCNGG | 6 cut(s) | 155, 186, 307, 942, 1490, 1841 |
| BstSFI | CTRYAG | 1 cut(s) | 546 |
| BstUI | CGCG | 2 cut(s) | 1142, 1706 |
| BstV1I | GCAGC | 4 cut(s) | 163, 541, 1408, 1522 |
| BstX2I | RGATCY | 2 cut(s) | 946, 1040 |
| BstYI | RGATCY | 2 cut(s) | 946, 1040 |
| Bsu15I | ATCGAT | 2 cut(s) | 618, 876 |
| Bsu36I | CCTNAGG | 1 cut(s) | 366 |
| BsuRI | GGCC | 4 cut(s) | 286, 416, 1145, 1410 |
| BsuTUI | ATCGAT | 2 cut(s) | 618, 876 |
| BtsCI | GGATG | 5 cut(s) | 502, 997, 1210, 1260, 1612 |
| BtsI | GCAGTG | 1 cut(s) | 1861 |
| BtsIMutI | CAGTG | 2 cut(s) | 256, 1861 |
| Cac8I | GCNNGC | 2 cut(s) | 514, 1704 |
| CaiI | CAGNNNCTG | 1 cut(s) | 554 |
| CfoI | GCGC | 1 cut(s) | 1142 |
| Cfr10I | RCCGGY | 1 cut(s) | 512 |
| Cfr13I | GGNCC | 2 cut(s) | 26, 305 |
| ClaI | ATCGAT | 2 cut(s) | 618, 876 |
| Csp6I | GTAC | 5 cut(s) | 296, 633, 749, 1061, 1594 |
| CviAII | CATG | 6 cut(s) | 63, 83, 135, 274, 517, 1666 |
| CviQI | GTAC | 5 cut(s) | 296, 633, 749, 1061, 1594 |
| DdeI | CTNAG | 4 cut(s) | 366, 1341, 1405, 1773 |
| EaeI | YGGCCR | 1 cut(s) | 1143 |
| Eam1104I | CTCTTC | 1 cut(s) | 657 |
| EarI | CTCTTC | 1 cut(s) | 657 |
| Ecl136II | GAGCTC | 2 cut(s) | 937, 1702 |
| Eco130I | CCWWGG | 3 cut(s) | 46, 706, 1222 |
| Eco147I | AGGCCT | 2 cut(s) | 416, 1410 |
| Eco24I | GRGCYC | 2 cut(s) | 939, 1704 |
| Eco32I | GATATC | 1 cut(s) | 375 |
| Eco47I | GGWCC | 2 cut(s) | 26, 305 |
| Eco53kI | GAGCTC | 2 cut(s) | 937, 1702 |
| Eco57I | CTGAAG | 1 cut(s) | 243 |
| Eco81I | CCTNAGG | 1 cut(s) | 366 |
| Eco88I | CYCGRG | 1 cut(s) | 1697 |
| EcoICRI | GAGCTC | 2 cut(s) | 937, 1702 |
| EcoRI | GAATTC | 2 cut(s) | 1050, 1613 |
| EcoRII | CCWGG | 5 cut(s) | 186, 307, 942, 1490, 1841 |
| EcoRV | GATATC | 1 cut(s) | 375 |
| EcoT14I | CCWWGG | 3 cut(s) | 46, 706, 1222 |
| EcoT38I | GRGCYC | 2 cut(s) | 939, 1704 |
| ErhI | CCWWGG | 3 cut(s) | 46, 706, 1222 |
| FaeI | CATG | 6 cut(s) | 66, 86, 138, 277, 520, 1669 |
| FaqI | GGGAC | 2 cut(s) | 631, 1508 |
| FatI | CATG | 6 cut(s) | 62, 82, 134, 273, 516, 1665 |
| FauNDI | CATATG | 2 cut(s) | 565, 1135 |
| FbaI | TGATCA | 1 cut(s) | 109 |
| Fnu4HI | GCNGC | 7 cut(s) | 12, 177, 552, 555, 1143, 1422, 1511 |
| FokI | GGATG | 5 cut(s) | 509, 1004, 1217, 1247, 1619 |
| FriOI | GRGCYC | 2 cut(s) | 939, 1704 |
| Fsp4HI | GCNGC | 7 cut(s) | 12, 177, 552, 555, 1143, 1422, 1511 |
| FspBI | CTAG | 4 cut(s) | 623, 1047, 1418, 1808 |
| GlaI | GCGC | 1 cut(s) | 1141 |
| GluI | GCNGC | 7 cut(s) | 12, 177, 552, 555, 1143, 1422, 1511 |
| GsuI | CTGGAG | 1 cut(s) | 714 |
| HaeIII | GGCC | 4 cut(s) | 286, 416, 1145, 1410 |
| HapII | CCGG | 2 cut(s) | 156, 513 |
| HhaI | GCGC | 1 cut(s) | 1142 |
| Hin1II | CATG | 6 cut(s) | 66, 86, 138, 277, 520, 1669 |
| Hin6I | GCGC | 1 cut(s) | 1140 |
| HinP1I | GCGC | 1 cut(s) | 1140 |
| HincII | GTYRAC | 1 cut(s) | 739 |
| HindII | GTYRAC | 1 cut(s) | 739 |
| HinfI | GANTC | 2 cut(s) | 473, 878 |
| HpaII | CCGG | 2 cut(s) | 156, 513 |
| HphI | GGTGA | 5 cut(s) | 32, 182, 691, 1367, 1859 |
| Hpy166II | GTNNAC | 1 cut(s) | 739 |
| Hpy188I | TCNGA | 3 cut(s) | 425, 1326, 1797 |
| Hpy188III | TCNNGA | 6 cut(s) | 365, 871, 1118, 1187, 1456, 1697 |
| Hpy8I | GTNNAC | 1 cut(s) | 739 |
| Hpy99I | CGWCG | 1 cut(s) | 303 |
| HpyAV | CCTTC | 2 cut(s) | 801, 1174 |
| HpyCH4III | ACNGT | 7 cut(s) | 132, 454, 971, 1091, 1312, 1678, 1749 |
| HpyCH4IV | ACGT | 1 cut(s) | 298 |
| HpyF10VI | GCNNNNNNNGC | 5 cut(s) | 11, 560, 1139, 1151, 1640 |
| HpyF3I | CTNAG | 4 cut(s) | 366, 1341, 1405, 1773 |
| HpySE526I | ACGT | 1 cut(s) | 298 |
| Hsp92II | CATG | 6 cut(s) | 66, 86, 138, 277, 520, 1669 |
| HspAI | GCGC | 1 cut(s) | 1140 |
| KroI | GCCGGC | 1 cut(s) | 512 |
| KroNI | GCCGGC | 1 cut(s) | 514 |
| Ksp22I | TGATCA | 1 cut(s) | 109 |
| LmnI | GCTCC | 2 cut(s) | 934, 1096 |
| Lsp1109I | GCAGC | 4 cut(s) | 163, 541, 1408, 1522 |
| LweI | GCATC | 8 cut(s) | 163, 211, 244, 387, 487, 982, 1119, 1453 |
| MaeI | CTAG | 4 cut(s) | 623, 1047, 1418, 1808 |
| MaeII | ACGT | 1 cut(s) | 298 |
| MaeIII | GTNAC | 5 cut(s) | 269, 448, 470, 1207, 1778 |
| MbiI | CCGCTC | 1 cut(s) | 1167 |
| MboII | GAAGA | 9 cut(s) | 359, 369, 380, 613, 674, 728, 840, 1582, 1726 |
| MfeI | CAATTG | 1 cut(s) | 558 |
| MflI | RGATCY | 2 cut(s) | 946, 1040 |
| MhlI | GDGCHC | 2 cut(s) | 939, 1704 |
| MlyI | GAGTC | 1 cut(s) | 467 |
| MmeI | TCCRAC | 1 cut(s) | 1438 |
| MroNI | GCCGGC | 1 cut(s) | 512 |
| MseI | TTAA | 4 cut(s) | 264, 393, 1086, 1734 |
| MslI | CAYNNNNRTG | 2 cut(s) | 87, 133 |
| MspA1I | CMGCKG | 1 cut(s) | 554 |
| MspCI | CTTAAG | 1 cut(s) | 392 |
| MspI | CCGG | 2 cut(s) | 156, 513 |
| MspR9I | CCNGG | 6 cut(s) | 157, 188, 309, 944, 1492, 1843 |
| MunI | CAATTG | 1 cut(s) | 558 |
| Mva1269I | GAATGC | 1 cut(s) | 1732 |
| MvaI | CCWGG | 5 cut(s) | 188, 309, 944, 1492, 1843 |
| MvnI | CGCG | 2 cut(s) | 1142, 1706 |
| MwoI | GCNNNNNNNGC | 5 cut(s) | 11, 560, 1139, 1151, 1640 |
| NaeI | GCCGGC | 1 cut(s) | 514 |
| NciI | CCSGG | 1 cut(s) | 157 |
| NdeI | CATATG | 2 cut(s) | 565, 1135 |
| NgoMIV | GCCGGC | 1 cut(s) | 512 |
| NlaIII | CATG | 6 cut(s) | 66, 86, 138, 277, 520, 1669 |
| NlaIV | GGNNCC | 2 cut(s) | 27, 1171 |
| NmuCI | GTSAC | 4 cut(s) | 269, 470, 1207, 1778 |
| PaeR7I | CTCGAG | 1 cut(s) | 1697 |
| PceI | AGGCCT | 2 cut(s) | 416, 1410 |
| PctI | GAATGC | 1 cut(s) | 1732 |
| PdiI | GCCGGC | 1 cut(s) | 514 |
| PfeI | GAWTC | 1 cut(s) | 878 |
| PflFI | GACNNNGTC | 1 cut(s) | 130 |
| PkrI | GCNGC | 7 cut(s) | 13, 178, 553, 556, 1144, 1423, 1512 |
| PleI | GAGTC | 1 cut(s) | 467 |
| PpsI | GAGTC | 1 cut(s) | 467 |
| Psp124BI | GAGCTC | 2 cut(s) | 939, 1704 |
| Psp6I | CCWGG | 5 cut(s) | 186, 307, 942, 1490, 1841 |
| PspGI | CCWGG | 5 cut(s) | 186, 307, 942, 1490, 1841 |
| PspN4I | GGNNCC | 2 cut(s) | 27, 1171 |
| PspPI | GGNCC | 2 cut(s) | 26, 305 |
| PstNI | CAGNNNCTG | 1 cut(s) | 554 |
| PsuI | RGATCY | 2 cut(s) | 946, 1040 |
| PsyI | GACNNNGTC | 1 cut(s) | 130 |
| RsaI | GTAC | 5 cut(s) | 297, 634, 750, 1062, 1595 |
| RsaNI | GTAC | 5 cut(s) | 296, 633, 749, 1061, 1594 |
| RseI | CAYNNNNRTG | 2 cut(s) | 87, 133 |
| SacI | GAGCTC | 2 cut(s) | 939, 1704 |
| SaqAI | TTAA | 4 cut(s) | 264, 393, 1086, 1734 |
| SatI | GCNGC | 7 cut(s) | 12, 177, 552, 555, 1143, 1422, 1511 |
| Sau96I | GGNCC | 2 cut(s) | 26, 305 |
| SchI | GAGTC | 1 cut(s) | 467 |
| ScrFI | CCNGG | 6 cut(s) | 157, 188, 309, 944, 1492, 1843 |
| SduI | GDGCHC | 2 cut(s) | 939, 1704 |
| SfaNI | GCATC | 8 cut(s) | 163, 211, 244, 387, 487, 982, 1119, 1453 |
| SfcI | CTRYAG | 1 cut(s) | 546 |
| Sfr274I | CTCGAG | 1 cut(s) | 1697 |
| SinI | GGWCC | 2 cut(s) | 26, 305 |
| SlaI | CTCGAG | 1 cut(s) | 1697 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 87, 133 |
| SmlI | CTYRAG | 2 cut(s) | 392, 1697 |
| SmoI | CTYRAG | 2 cut(s) | 392, 1697 |
| SseBI | AGGCCT | 2 cut(s) | 416, 1410 |
| SsiI | CCGC | 5 cut(s) | 552, 1142, 1167, 1628, 1706 |
| SspMI | CTAG | 4 cut(s) | 623, 1047, 1418, 1808 |
| SstI | GAGCTC | 2 cut(s) | 939, 1704 |
| StuI | AGGCCT | 2 cut(s) | 416, 1410 |
| StyD4I | CCNGG | 6 cut(s) | 155, 186, 307, 942, 1490, 1841 |
| StyI | CCWWGG | 3 cut(s) | 46, 706, 1222 |
| TaaI | ACNGT | 7 cut(s) | 132, 454, 971, 1091, 1312, 1678, 1749 |
| TaiI | ACGT | 1 cut(s) | 301 |
| TaqI | TCGA | 9 cut(s) | 42, 618, 639, 723, 870, 876, 975, 1455, 1698 |
| TatI | WGTACW | 2 cut(s) | 748, 1593 |
| TauI | GCSGC | 2 cut(s) | 554, 1145 |
| TfiI | GAWTC | 1 cut(s) | 878 |
| Tru1I | TTAA | 4 cut(s) | 264, 393, 1086, 1734 |
| Tru9I | TTAA | 4 cut(s) | 264, 393, 1086, 1734 |
| TscAI | CASTG | 2 cut(s) | 256, 1861 |
| TseFI | GTSAC | 4 cut(s) | 269, 470, 1207, 1778 |
| TseI | GCWGC | 5 cut(s) | 11, 176, 554, 1421, 1510 |
| Tsp45I | GTSAC | 4 cut(s) | 269, 470, 1207, 1778 |
| TspDTI | ATGAA | 7 cut(s) | 212, 290, 360, 533, 1137, 1606, 1773 |
| TspGWI | ACGGA | 2 cut(s) | 1004, 1485 |
| TspRI | CASTG | 2 cut(s) | 256, 1861 |
| Tth111I | GACNNNGTC | 1 cut(s) | 130 |
| Vha464I | CTTAAG | 1 cut(s) | 392 |
| VpaK11BI | GGWCC | 2 cut(s) | 26, 305 |
| XapI | RAATTY | 6 cut(s) | 245, 669, 926, 1050, 1473, 1613 |
| XhoI | CTCGAG | 1 cut(s) | 1697 |
| XspI | CTAG | 4 cut(s) | 623, 1047, 1418, 1808 |