Belongs to the heat shock protein 70 family
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 2 cut(s) | 1242, 1623 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 35 |
| AccBSI | CCGCTC | 1 cut(s) | 854 |
| AciI | CCGC | 7 cut(s) | 546, 552, 594, 852, 1124, 1491, 1736 |
| AclI | AACGTT | 1 cut(s) | 603 |
| AclWI | GGATC | 3 cut(s) | 430, 951, 1045 |
| AcsI | RAATTY | 4 cut(s) | 245, 353, 666, 923 |
| AcuI | CTGAAG | 1 cut(s) | 384 |
| AfaI | GTAC | 5 cut(s) | 631, 747, 1146, 1396, 1601 |
| AfeI | AGCGCT | 1 cut(s) | 230 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 35, 264 |
| AflIII | ACRYGT | 1 cut(s) | 1602 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 1373, 1405 |
| AleI | CACNNNNGTG | 1 cut(s) | 87 |
| AloI | GAACNNNNNNTCC | 2 cut(s) | 588, 620 |
| AluBI | AGCT | 8 cut(s) | 104, 683, 689, 1096, 1151, 1405, 1810, 1819 |
| AluI | AGCT | 8 cut(s) | 104, 683, 689, 1096, 1151, 1405, 1810, 1819 |
| Alw26I | GTCTC | 1 cut(s) | 1492 |
| AlwI | GGATC | 3 cut(s) | 430, 951, 1045 |
| Aor51HI | AGCGCT | 1 cut(s) | 230 |
| AoxI | GGCC | 2 cut(s) | 284, 483 |
| ApeKI | GCWGC | 4 cut(s) | 176, 359, 548, 1151 |
| ApoI | RAATTY | 4 cut(s) | 245, 353, 666, 923 |
| Asp700I | GAANNNNTTC | 2 cut(s) | 740, 841 |
| AspLEI | GCGC | 1 cut(s) | 231 |
| AspS9I | GGNCC | 1 cut(s) | 26 |
| AsuHPI | GGTGA | 5 cut(s) | 182, 688, 722, 745, 1525 |
| AvaII | GGWCC | 1 cut(s) | 26 |
| BauI | CACGAG | 1 cut(s) | 61 |
| BbsI | GAAGAC | 1 cut(s) | 1719 |
| BbvI | GCAGC | 4 cut(s) | 188, 346, 535, 1138 |
| BccI | CCATC | 3 cut(s) | 1289, 1573, 1669 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 68, 102 |
| BciVI | GTATCC | 2 cut(s) | 202, 1346 |
| BclI | TGATCA | 1 cut(s) | 109 |
| BcoDI | GTCTC | 1 cut(s) | 1492 |
| BfaI | CTAG | 1 cut(s) | 1044 |
| BfmI | CTRYAG | 1 cut(s) | 684 |
| BfoI | RGCGCY | 1 cut(s) | 232 |
| BfuI | GTATCC | 2 cut(s) | 202, 1346 |
| BglII | AGATCT | 1 cut(s) | 1075 |
| BisI | GCNGC | 5 cut(s) | 177, 360, 549, 552, 1152 |
| BlsI | GCNGC | 5 cut(s) | 178, 361, 550, 553, 1153 |
| Bme18I | GGWCC | 1 cut(s) | 26 |
| BmgT120I | GGNCC | 1 cut(s) | 26 |
| BmiI | GGNNCC | 2 cut(s) | 27, 966 |
| BmsI | GCATC | 9 cut(s) | 163, 211, 244, 384, 484, 979, 1116, 1456, 1723 |
| BpiI | GAAGAC | 1 cut(s) | 1719 |
| BpmI | CTGGAG | 1 cut(s) | 711 |
| BpuEI | CTTGAG | 1 cut(s) | 635 |
| BsaBI | GATNNNNATC | 3 cut(s) | 38, 699, 1529 |
| BsaJI | CCNNGG | 2 cut(s) | 46, 1494 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 35, 264 |
| Bse118I | RCCGGY | 1 cut(s) | 497 |
| Bse1I | ACTGG | 1 cut(s) | 1339 |
| Bse3DI | GCAATG | 1 cut(s) | 555 |
| Bse8I | GATNNNNATC | 3 cut(s) | 38, 699, 1529 |
| BseDI | CCNNGG | 2 cut(s) | 46, 1494 |
| BseGI | GGATG | 6 cut(s) | 499, 519, 1213, 1281, 1413, 1738 |
| BseJI | GATNNNNATC | 3 cut(s) | 38, 699, 1529 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 35, 264 |
| BseMI | GCAATG | 1 cut(s) | 555 |
| BseNI | ACTGG | 1 cut(s) | 1339 |
| BseXI | GCAGC | 4 cut(s) | 188, 346, 535, 1138 |
| BsgI | GTGCAG | 2 cut(s) | 1308, 1852 |
| Bsh1285I | CGRYCG | 2 cut(s) | 88, 1143 |
| BshFI | GGCC | 2 cut(s) | 286, 485 |
| BsiEI | CGRYCG | 2 cut(s) | 88, 1143 |
| BsiSI | CCGG | 1 cut(s) | 498 |
| BslFI | GGGAC | 2 cut(s) | 1250, 1821 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 35, 264 |
| BsmAI | GTCTC | 1 cut(s) | 1492 |
| BsmFI | GGGAC | 2 cut(s) | 1250, 1821 |
| BsnI | GGCC | 2 cut(s) | 286, 485 |
| Bsp143I | GATC | 9 cut(s) | 109, 234, 422, 616, 943, 1037, 1075, 1301, 1524 |
| BspACI | CCGC | 7 cut(s) | 546, 552, 594, 852, 1124, 1491, 1736 |
| BspANI | GGCC | 2 cut(s) | 286, 485 |
| BspHI | TCATGA | 1 cut(s) | 1015 |
| BspLI | GGNNCC | 2 cut(s) | 27, 966 |
| BspPI | GGATC | 3 cut(s) | 430, 951, 1045 |
| BsrBI | CCGCTC | 1 cut(s) | 854 |
| BsrDI | GCAATG | 1 cut(s) | 555 |
| BsrFI | RCCGGY | 1 cut(s) | 497 |
| BsrI | ACTGG | 1 cut(s) | 1339 |
| BssAI | RCCGGY | 1 cut(s) | 497 |
| BssECI | CCNNGG | 2 cut(s) | 46, 1494 |
| BssMI | GATC | 9 cut(s) | 109, 234, 422, 616, 943, 1037, 1075, 1301, 1524 |
| BssSI | CACGAG | 1 cut(s) | 61 |
| Bst2BI | CACGAG | 1 cut(s) | 61 |
| Bst4CI | ACNGT | 9 cut(s) | 86, 132, 214, 430, 451, 589, 1144, 1315, 1684 |
| Bst6I | CTCTTC | 2 cut(s) | 360, 654 |
| BstAPI | GCANNNNNTGC | 2 cut(s) | 1136, 1557 |
| BstDEI | CTNAG | 1 cut(s) | 800 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 262 |
| BstF5I | GGATG | 6 cut(s) | 499, 519, 1213, 1281, 1413, 1738 |
| BstH2I | RGCGCY | 1 cut(s) | 232 |
| BstHHI | GCGC | 1 cut(s) | 231 |
| BstKTI | GATC | 9 cut(s) | 112, 237, 425, 619, 946, 1040, 1078, 1304, 1527 |
| BstMAI | GTCTC | 1 cut(s) | 1492 |
| BstMBI | GATC | 9 cut(s) | 109, 234, 422, 616, 943, 1037, 1075, 1301, 1524 |
| BstMCI | CGRYCG | 2 cut(s) | 88, 1143 |
| BstMWI | GCNNNNNNNGC | 5 cut(s) | 557, 1136, 1557, 1646, 1816 |
| BstNSI | RCATGY | 1 cut(s) | 1606 |
| BstSFI | CTRYAG | 1 cut(s) | 684 |
| BstV1I | GCAGC | 4 cut(s) | 188, 346, 535, 1138 |
| BstV2I | GAAGAC | 1 cut(s) | 1719 |
| BstX2I | RGATCY | 3 cut(s) | 943, 1037, 1075 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 1675 |
| BstYI | RGATCY | 3 cut(s) | 943, 1037, 1075 |
| BsuI | GTATCC | 2 cut(s) | 202, 1346 |
| BsuRI | GGCC | 2 cut(s) | 286, 485 |
| BtgZI | GCGATG | 1 cut(s) | 267 |
| BtsCI | GGATG | 6 cut(s) | 499, 519, 1213, 1281, 1413, 1738 |
| BtsIMutI | CAGTG | 1 cut(s) | 210 |
| CciI | TCATGA | 1 cut(s) | 1015 |
| CfoI | GCGC | 1 cut(s) | 231 |
| Cfr10I | RCCGGY | 1 cut(s) | 497 |
| Cfr13I | GGNCC | 1 cut(s) | 26 |
| Csp6I | GTAC | 5 cut(s) | 630, 746, 1145, 1395, 1600 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 280, 315 |
| CviAII | CATG | 8 cut(s) | 274, 319, 379, 767, 940, 1016, 1332, 1603 |
| CviQI | GTAC | 5 cut(s) | 630, 746, 1145, 1395, 1600 |
| DdeI | CTNAG | 1 cut(s) | 800 |
| DpnI | GATC | 9 cut(s) | 111, 236, 424, 618, 945, 1039, 1077, 1303, 1526 |
| DpnII | GATC | 9 cut(s) | 109, 234, 422, 616, 943, 1037, 1075, 1301, 1524 |
| Eam1104I | CTCTTC | 2 cut(s) | 360, 654 |
| EarI | CTCTTC | 2 cut(s) | 360, 654 |
| EciI | GGCGGA | 2 cut(s) | 609, 1480 |
| Eco32I | GATATC | 4 cut(s) | 372, 778, 934, 1444 |
| Eco47I | GGWCC | 1 cut(s) | 26 |
| Eco47III | AGCGCT | 1 cut(s) | 230 |
| Eco57I | CTGAAG | 1 cut(s) | 384 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 262 |
| EcoRV | GATATC | 4 cut(s) | 372, 778, 934, 1444 |
| FaeI | CATG | 8 cut(s) | 277, 322, 382, 770, 943, 1019, 1335, 1606 |
| FaqI | GGGAC | 2 cut(s) | 1250, 1821 |
| FatI | CATG | 8 cut(s) | 273, 318, 378, 766, 939, 1015, 1331, 1602 |
| FauNDI | CATATG | 2 cut(s) | 1132, 1251 |
| FbaI | TGATCA | 1 cut(s) | 109 |
| Fnu4HI | GCNGC | 5 cut(s) | 177, 360, 549, 552, 1152 |
| FokI | GGATG | 6 cut(s) | 506, 526, 1220, 1268, 1400, 1745 |
| Fsp4HI | GCNGC | 5 cut(s) | 177, 360, 549, 552, 1152 |
| FspBI | CTAG | 1 cut(s) | 1044 |
| GlaI | GCGC | 1 cut(s) | 230 |
| GluI | GCNGC | 5 cut(s) | 177, 360, 549, 552, 1152 |
| GsuI | CTGGAG | 1 cut(s) | 711 |
| HaeII | RGCGCY | 1 cut(s) | 232 |
| HaeIII | GGCC | 2 cut(s) | 286, 485 |
| HapII | CCGG | 1 cut(s) | 498 |
| HhaI | GCGC | 1 cut(s) | 231 |
| Hin1II | CATG | 8 cut(s) | 277, 322, 382, 770, 943, 1019, 1335, 1606 |
| Hin6I | GCGC | 1 cut(s) | 229 |
| HinP1I | GCGC | 1 cut(s) | 229 |
| HincII | GTYRAC | 1 cut(s) | 331 |
| HindII | GTYRAC | 1 cut(s) | 331 |
| HinfI | GANTC | 8 cut(s) | 184, 382, 736, 875, 1047, 1476, 1725, 1774 |
| HpaI | GTTAAC | 1 cut(s) | 331 |
| HpaII | CCGG | 1 cut(s) | 498 |
| HphI | GGTGA | 5 cut(s) | 182, 688, 722, 745, 1525 |
| Hpy166II | GTNNAC | 2 cut(s) | 331, 973 |
| Hpy188I | TCNGA | 3 cut(s) | 50, 422, 1609 |
| Hpy188III | TCNNGA | 8 cut(s) | 569, 652, 757, 1016, 1115, 1190, 1439, 1480 |
| Hpy8I | GTNNAC | 2 cut(s) | 331, 973 |
| Hpy99I | CGWCG | 2 cut(s) | 641, 1319 |
| HpyAV | CCTTC | 1 cut(s) | 656 |
| HpyCH4III | ACNGT | 9 cut(s) | 86, 132, 214, 430, 451, 589, 1144, 1315, 1684 |
| HpyCH4IV | ACGT | 4 cut(s) | 138, 478, 603, 639 |
| HpyF10VI | GCNNNNNNNGC | 5 cut(s) | 557, 1136, 1557, 1646, 1816 |
| HpyF3I | CTNAG | 1 cut(s) | 800 |
| HpySE526I | ACGT | 4 cut(s) | 138, 478, 603, 639 |
| Hsp92II | CATG | 8 cut(s) | 277, 322, 382, 770, 943, 1019, 1335, 1606 |
| HspAI | GCGC | 1 cut(s) | 229 |
| Ksp22I | TGATCA | 1 cut(s) | 109 |
| KspAI | GTTAAC | 1 cut(s) | 331 |
| Kzo9I | GATC | 9 cut(s) | 109, 234, 422, 616, 943, 1037, 1075, 1301, 1524 |
| LmnI | GCTCC | 2 cut(s) | 964, 1093 |
| Lsp1109I | GCAGC | 4 cut(s) | 188, 346, 535, 1138 |
| LweI | GCATC | 9 cut(s) | 163, 211, 244, 384, 484, 979, 1116, 1456, 1723 |
| MaeI | CTAG | 1 cut(s) | 1044 |
| MaeII | ACGT | 4 cut(s) | 138, 478, 603, 639 |
| MaeIII | GTNAC | 5 cut(s) | 269, 445, 1210, 1306, 1318 |
| MalI | GATC | 9 cut(s) | 111, 236, 424, 618, 945, 1039, 1077, 1303, 1526 |
| MbiI | CCGCTC | 1 cut(s) | 854 |
| MboI | GATC | 9 cut(s) | 109, 234, 422, 616, 943, 1037, 1075, 1301, 1524 |
| MflI | RGATCY | 3 cut(s) | 943, 1037, 1075 |
| MluCI | AATT | 8 cut(s) | 165, 245, 353, 504, 666, 923, 1155, 1569 |
| MmeI | TCCRAC | 2 cut(s) | 53, 1185 |
| MroXI | GAANNNNTTC | 2 cut(s) | 740, 841 |
| MseI | TTAA | 4 cut(s) | 164, 330, 390, 1083 |
| MslI | CAYNNNNRTG | 2 cut(s) | 87, 1330 |
| MspA1I | CMGCKG | 2 cut(s) | 548, 689 |
| MspI | CCGG | 1 cut(s) | 498 |
| MwoI | GCNNNNNNNGC | 5 cut(s) | 557, 1136, 1557, 1646, 1816 |
| NdeI | CATATG | 2 cut(s) | 1132, 1251 |
| NdeII | GATC | 9 cut(s) | 109, 234, 422, 616, 943, 1037, 1075, 1301, 1524 |
| NlaIII | CATG | 8 cut(s) | 277, 322, 382, 770, 943, 1019, 1335, 1606 |
| NlaIV | GGNNCC | 2 cut(s) | 27, 966 |
| NmeAIII | GCCGAG | 1 cut(s) | 1519 |
| NmuCI | GTSAC | 3 cut(s) | 269, 1210, 1306 |
| NspI | RCATGY | 1 cut(s) | 1606 |
| OliI | CACNNNNGTG | 1 cut(s) | 87 |
| PagI | TCATGA | 1 cut(s) | 1015 |
| PciI | ACATGT | 1 cut(s) | 1602 |
| PdmI | GAANNNNTTC | 2 cut(s) | 740, 841 |
| PfeI | GAWTC | 8 cut(s) | 184, 382, 736, 875, 1047, 1476, 1725, 1774 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 35 |
| PkrI | GCNGC | 5 cut(s) | 178, 361, 550, 553, 1153 |
| PscI | ACATGT | 1 cut(s) | 1602 |
| PsiI | TTATAA | 2 cut(s) | 1242, 1623 |
| Psp1406I | AACGTT | 1 cut(s) | 603 |
| PspN4I | GGNNCC | 2 cut(s) | 27, 966 |
| PspPI | GGNCC | 1 cut(s) | 26 |
| PsuI | RGATCY | 3 cut(s) | 943, 1037, 1075 |
| PvuII | CAGCTG | 1 cut(s) | 689 |
| RsaI | GTAC | 5 cut(s) | 631, 747, 1146, 1396, 1601 |
| RsaNI | GTAC | 5 cut(s) | 630, 746, 1145, 1395, 1600 |
| RseI | CAYNNNNRTG | 2 cut(s) | 87, 1330 |
| SaqAI | TTAA | 4 cut(s) | 164, 330, 390, 1083 |
| SatI | GCNGC | 5 cut(s) | 177, 360, 549, 552, 1152 |
| Sau3AI | GATC | 9 cut(s) | 109, 234, 422, 616, 943, 1037, 1075, 1301, 1524 |
| Sau96I | GGNCC | 1 cut(s) | 26 |
| SfaNI | GCATC | 9 cut(s) | 163, 211, 244, 384, 484, 979, 1116, 1456, 1723 |
| SfcI | CTRYAG | 1 cut(s) | 684 |
| SinI | GGWCC | 1 cut(s) | 26 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 87, 1330 |
| SmlI | CTYRAG | 1 cut(s) | 650 |
| SmoI | CTYRAG | 1 cut(s) | 650 |
| Sse9I | AATT | 8 cut(s) | 165, 245, 353, 504, 666, 923, 1155, 1569 |
| SsiI | CCGC | 7 cut(s) | 546, 552, 594, 852, 1124, 1491, 1736 |
| SspI | AATATT | 1 cut(s) | 520 |
| SspMI | CTAG | 1 cut(s) | 1044 |
| TaaI | ACNGT | 9 cut(s) | 86, 132, 214, 430, 451, 589, 1144, 1315, 1684 |
| TaiI | ACGT | 4 cut(s) | 141, 481, 606, 642 |
| TaqI | TCGA | 5 cut(s) | 42, 425, 636, 1584, 1821 |
| TasI | AATT | 8 cut(s) | 165, 245, 353, 504, 666, 923, 1155, 1569 |
| TatI | WGTACW | 1 cut(s) | 1599 |
| TauI | GCSGC | 1 cut(s) | 554 |
| TfiI | GAWTC | 8 cut(s) | 184, 382, 736, 875, 1047, 1476, 1725, 1774 |
| Tru1I | TTAA | 4 cut(s) | 164, 330, 390, 1083 |
| Tru9I | TTAA | 4 cut(s) | 164, 330, 390, 1083 |
| TscAI | CASTG | 1 cut(s) | 217 |
| TseFI | GTSAC | 3 cut(s) | 269, 1210, 1306 |
| TseI | GCWGC | 4 cut(s) | 176, 359, 548, 1151 |
| Tsp45I | GTSAC | 3 cut(s) | 269, 1210, 1306 |
| TspDTI | ATGAA | 6 cut(s) | 290, 530, 783, 1004, 1134, 1676 |
| TspGWI | ACGGA | 3 cut(s) | 515, 1406, 1649 |
| TspRI | CASTG | 1 cut(s) | 217 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 35 |
| VpaK11BI | GGWCC | 1 cut(s) | 26 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 262 |
| XapI | RAATTY | 4 cut(s) | 245, 353, 666, 923 |
| XceI | RCATGY | 1 cut(s) | 1606 |
| XmnI | GAANNNNTTC | 2 cut(s) | 740, 841 |
| XspI | CTAG | 1 cut(s) | 1044 |