Belongs to the heat shock protein 70 family
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccB1I | GGYRCC | 1 cut(s) | 758 |
| AccI | GTMKAC | 1 cut(s) | 864 |
| AccIII | TCCGGA | 1 cut(s) | 736 |
| AciI | CCGC | 2 cut(s) | 19, 762 |
| AclWI | GGATC | 1 cut(s) | 667 |
| AcsI | RAATTY | 3 cut(s) | 545, 669, 1072 |
| AcuI | CTGAAG | 1 cut(s) | 459 |
| AfaI | GTAC | 2 cut(s) | 253, 997 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 924 |
| AgsI | TTSAA | 7 cut(s) | 88, 296, 379, 491, 607, 706, 899 |
| AjiI | CACGTC | 1 cut(s) | 400 |
| AluBI | AGCT | 3 cut(s) | 773, 805, 1054 |
| AluI | AGCT | 3 cut(s) | 773, 805, 1054 |
| Alw21I | GWGCWC | 1 cut(s) | 421 |
| Alw26I | GTCTC | 3 cut(s) | 309, 454, 869 |
| AlwI | GGATC | 1 cut(s) | 667 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 482 |
| Aor13HI | TCCGGA | 1 cut(s) | 736 |
| AoxI | GGCC | 3 cut(s) | 33, 133, 303 |
| ApeKI | GCWGC | 6 cut(s) | 123, 170, 173, 682, 773, 1066 |
| ApoI | RAATTY | 3 cut(s) | 545, 669, 1072 |
| AsuHPI | GGTGA | 3 cut(s) | 293, 314, 1001 |
| BanI | GGYRCC | 1 cut(s) | 758 |
| BbsI | GAAGAC | 1 cut(s) | 342 |
| Bbv12I | GWGCWC | 1 cut(s) | 421 |
| BbvCI | CCTCAGC | 1 cut(s) | 30 |
| BbvI | GCAGC | 6 cut(s) | 135, 157, 185, 694, 760, 1053 |
| BccI | CCATC | 2 cut(s) | 278, 926 |
| BceAI | ACGGC | 1 cut(s) | 324 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 149, 183 |
| BcoDI | GTCTC | 3 cut(s) | 309, 454, 869 |
| BfaI | CTAG | 5 cut(s) | 8, 414, 584, 666, 845 |
| BfmI | CTRYAG | 2 cut(s) | 171, 774 |
| BisI | GCNGC | 7 cut(s) | 124, 171, 174, 683, 762, 774, 1067 |
| BlsI | GCNGC | 7 cut(s) | 125, 172, 175, 684, 763, 775, 1068 |
| BmgBI | CACGTC | 1 cut(s) | 400 |
| BmiI | GGNNCC | 1 cut(s) | 760 |
| BmsI | GCATC | 2 cut(s) | 601, 1086 |
| BpiI | GAAGAC | 1 cut(s) | 342 |
| BpmI | CTGGAG | 2 cut(s) | 333, 1034 |
| Bpu10I | CCTNAGC | 1 cut(s) | 30 |
| BpuEI | CTTGAG | 2 cut(s) | 802, 1057 |
| BsaBI | GATNNNNATC | 1 cut(s) | 149 |
| BsaJI | CCNNGG | 3 cut(s) | 306, 325, 1057 |
| BsaWI | WCCGGW | 1 cut(s) | 736 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 924 |
| Bse1I | ACTGG | 1 cut(s) | 590 |
| Bse3DI | GCAATG | 1 cut(s) | 126 |
| Bse8I | GATNNNNATC | 1 cut(s) | 149 |
| BseAI | TCCGGA | 1 cut(s) | 736 |
| BseDI | CCNNGG | 3 cut(s) | 306, 325, 1057 |
| BseGI | GGATG | 4 cut(s) | 616, 703, 745, 832 |
| BseJI | GATNNNNATC | 1 cut(s) | 149 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 924 |
| BseMI | GCAATG | 1 cut(s) | 126 |
| BseMII | CTCAG | 1 cut(s) | 21 |
| BseNI | ACTGG | 1 cut(s) | 590 |
| BseRI | GAGGAG | 2 cut(s) | 345, 472 |
| BseXI | GCAGC | 6 cut(s) | 135, 157, 185, 694, 760, 1053 |
| BseYI | CCCAGC | 1 cut(s) | 203 |
| BsgI | GTGCAG | 1 cut(s) | 701 |
| BshFI | GGCC | 3 cut(s) | 35, 135, 305 |
| BshNI | GGYRCC | 1 cut(s) | 758 |
| BsiHKAI | GWGCWC | 1 cut(s) | 421 |
| BsiSI | CCGG | 1 cut(s) | 737 |
| BslFI | GGGAC | 1 cut(s) | 1075 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 924 |
| BsmAI | GTCTC | 3 cut(s) | 309, 454, 869 |
| BsmFI | GGGAC | 1 cut(s) | 1075 |
| BsmI | GAATGC | 1 cut(s) | 64 |
| BsnI | GGCC | 3 cut(s) | 35, 135, 305 |
| Bsp1286I | GDGCHC | 1 cut(s) | 421 |
| Bsp13I | TCCGGA | 1 cut(s) | 736 |
| Bsp143I | GATC | 2 cut(s) | 659, 817 |
| BspACI | CCGC | 2 cut(s) | 19, 762 |
| BspANI | GGCC | 3 cut(s) | 35, 135, 305 |
| BspCNI | CTCAG | 1 cut(s) | 22 |
| BspEI | TCCGGA | 1 cut(s) | 736 |
| BspLI | GGNNCC | 1 cut(s) | 760 |
| BspMAI | CTGCAG | 2 cut(s) | 175, 778 |
| BspPI | GGATC | 1 cut(s) | 667 |
| BspT107I | GGYRCC | 1 cut(s) | 758 |
| BsrDI | GCAATG | 1 cut(s) | 126 |
| BsrI | ACTGG | 1 cut(s) | 590 |
| BssECI | CCNNGG | 3 cut(s) | 306, 325, 1057 |
| BssMI | GATC | 2 cut(s) | 659, 817 |
| BssT1I | CCWWGG | 2 cut(s) | 325, 1057 |
| Bst4CI | ACNGT | 4 cut(s) | 73, 500, 859, 1105 |
| BstAPI | GCANNNNNTGC | 2 cut(s) | 179, 691 |
| BstC8I | GCNNGC | 3 cut(s) | 121, 133, 771 |
| BstDEI | CTNAG | 2 cut(s) | 30, 422 |
| BstDSI | CCRYGG | 1 cut(s) | 306 |
| BstF5I | GGATG | 4 cut(s) | 616, 703, 745, 832 |
| BstKTI | GATC | 2 cut(s) | 662, 820 |
| BstMAI | GTCTC | 3 cut(s) | 309, 454, 869 |
| BstMBI | GATC | 2 cut(s) | 659, 817 |
| BstMWI | GCNNNNNNNGC | 5 cut(s) | 132, 179, 691, 758, 770 |
| BstNSI | RCATGY | 1 cut(s) | 444 |
| BstSFI | CTRYAG | 2 cut(s) | 171, 774 |
| BstV1I | GCAGC | 6 cut(s) | 135, 157, 185, 694, 760, 1053 |
| BstV2I | GAAGAC | 1 cut(s) | 342 |
| BstX2I | RGATCY | 1 cut(s) | 659 |
| BstYI | RGATCY | 1 cut(s) | 659 |
| BsuRI | GGCC | 3 cut(s) | 35, 135, 305 |
| BtgI | CCRYGG | 1 cut(s) | 306 |
| BtrI | CACGTC | 1 cut(s) | 400 |
| BtsCI | GGATG | 4 cut(s) | 616, 703, 745, 832 |
| BtsI | GCAGTG | 1 cut(s) | 637 |
| BtsIMutI | CAGTG | 3 cut(s) | 69, 480, 637 |
| Cac8I | GCNNGC | 3 cut(s) | 121, 133, 771 |
| CaiI | CAGNNNCTG | 1 cut(s) | 482 |
| CseI | GACGC | 1 cut(s) | 1072 |
| Csp6I | GTAC | 2 cut(s) | 252, 996 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 469, 504 |
| CviAII | CATG | 2 cut(s) | 441, 638 |
| CviQI | GTAC | 2 cut(s) | 252, 996 |
| DdeI | CTNAG | 2 cut(s) | 30, 422 |
| DpnI | GATC | 2 cut(s) | 661, 819 |
| DpnII | GATC | 2 cut(s) | 659, 817 |
| Eco130I | CCWWGG | 2 cut(s) | 325, 1057 |
| Eco147I | AGGCCT | 1 cut(s) | 35 |
| Eco57I | CTGAAG | 1 cut(s) | 459 |
| EcoRI | GAATTC | 1 cut(s) | 669 |
| EcoT14I | CCWWGG | 2 cut(s) | 325, 1057 |
| ErhI | CCWWGG | 2 cut(s) | 325, 1057 |
| FaeI | CATG | 2 cut(s) | 444, 641 |
| FaqI | GGGAC | 1 cut(s) | 1075 |
| FatI | CATG | 2 cut(s) | 440, 637 |
| FauI | CCCGC | 1 cut(s) | 12 |
| FblI | GTMKAC | 1 cut(s) | 864 |
| Fnu4HI | GCNGC | 7 cut(s) | 124, 171, 174, 683, 762, 774, 1067 |
| FokI | GGATG | 4 cut(s) | 623, 710, 752, 839 |
| Fsp4HI | GCNGC | 7 cut(s) | 124, 171, 174, 683, 762, 774, 1067 |
| FspBI | CTAG | 5 cut(s) | 8, 414, 584, 666, 845 |
| GluI | GCNGC | 7 cut(s) | 124, 171, 174, 683, 762, 774, 1067 |
| GsaI | CCCAGC | 1 cut(s) | 207 |
| GsuI | CTGGAG | 2 cut(s) | 333, 1034 |
| HaeIII | GGCC | 3 cut(s) | 35, 135, 305 |
| HapII | CCGG | 1 cut(s) | 737 |
| HgaI | GACGC | 1 cut(s) | 1072 |
| Hin1II | CATG | 2 cut(s) | 444, 641 |
| HincII | GTYRAC | 1 cut(s) | 970 |
| HindII | GTYRAC | 1 cut(s) | 970 |
| HinfI | GANTC | 2 cut(s) | 92, 895 |
| HpaII | CCGG | 1 cut(s) | 737 |
| HphI | GGTGA | 3 cut(s) | 293, 314, 1001 |
| Hpy166II | GTNNAC | 3 cut(s) | 397, 865, 970 |
| Hpy188I | TCNGA | 1 cut(s) | 855 |
| Hpy188III | TCNNGA | 7 cut(s) | 379, 414, 584, 666, 737, 781, 922 |
| Hpy8I | GTNNAC | 3 cut(s) | 397, 865, 970 |
| HpyAV | CCTTC | 3 cut(s) | 193, 295, 601 |
| HpyCH4III | ACNGT | 4 cut(s) | 73, 500, 859, 1105 |
| HpyCH4IV | ACGT | 2 cut(s) | 100, 399 |
| HpyCH4V | TGCA | 7 cut(s) | 62, 173, 682, 694, 723, 769, 776 |
| HpyF10VI | GCNNNNNNNGC | 5 cut(s) | 132, 179, 691, 758, 770 |
| HpyF3I | CTNAG | 2 cut(s) | 30, 422 |
| HpySE526I | ACGT | 2 cut(s) | 100, 399 |
| Hsp92II | CATG | 2 cut(s) | 444, 641 |
| Kpn2I | TCCGGA | 1 cut(s) | 736 |
| Kzo9I | GATC | 2 cut(s) | 659, 817 |
| LmnI | GCTCC | 1 cut(s) | 1059 |
| Lsp1109I | GCAGC | 6 cut(s) | 135, 157, 185, 694, 760, 1053 |
| LweI | GCATC | 2 cut(s) | 601, 1086 |
| MaeI | CTAG | 5 cut(s) | 8, 414, 584, 666, 845 |
| MaeII | ACGT | 2 cut(s) | 100, 399 |
| MaeIII | GTNAC | 2 cut(s) | 829, 840 |
| MalI | GATC | 2 cut(s) | 661, 819 |
| MboI | GATC | 2 cut(s) | 659, 817 |
| MboII | GAAGA | 4 cut(s) | 67, 347, 394, 654 |
| MfeI | CAATTG | 2 cut(s) | 177, 747 |
| MflI | RGATCY | 1 cut(s) | 659 |
| MhlI | GDGCHC | 1 cut(s) | 421 |
| MluCI | AATT | 9 cut(s) | 43, 177, 374, 545, 669, 747, 1015, 1027, 1072 |
| MroI | TCCGGA | 1 cut(s) | 736 |
| MseI | TTAA | 2 cut(s) | 431, 573 |
| MspA1I | CMGCKG | 1 cut(s) | 764 |
| MspI | CCGG | 1 cut(s) | 737 |
| MunI | CAATTG | 2 cut(s) | 177, 747 |
| Mva1269I | GAATGC | 1 cut(s) | 64 |
| MwoI | GCNNNNNNNGC | 5 cut(s) | 132, 179, 691, 758, 770 |
| NdeII | GATC | 2 cut(s) | 659, 817 |
| NlaIII | CATG | 2 cut(s) | 444, 641 |
| NlaIV | GGNNCC | 1 cut(s) | 760 |
| NmuCI | GTSAC | 2 cut(s) | 829, 840 |
| NspI | RCATGY | 1 cut(s) | 444 |
| PceI | AGGCCT | 1 cut(s) | 35 |
| PctI | GAATGC | 1 cut(s) | 64 |
| PfeI | GAWTC | 2 cut(s) | 92, 895 |
| PkrI | GCNGC | 7 cut(s) | 125, 172, 175, 684, 763, 775, 1068 |
| PspFI | CCCAGC | 1 cut(s) | 203 |
| PspN4I | GGNNCC | 1 cut(s) | 760 |
| PstI | CTGCAG | 2 cut(s) | 175, 778 |
| PstNI | CAGNNNCTG | 1 cut(s) | 482 |
| PsuI | RGATCY | 1 cut(s) | 659 |
| RsaI | GTAC | 2 cut(s) | 253, 997 |
| RsaNI | GTAC | 2 cut(s) | 252, 996 |
| SaqAI | TTAA | 2 cut(s) | 431, 573 |
| SatI | GCNGC | 7 cut(s) | 124, 171, 174, 683, 762, 774, 1067 |
| Sau3AI | GATC | 2 cut(s) | 659, 817 |
| SduI | GDGCHC | 1 cut(s) | 421 |
| SfaNI | GCATC | 2 cut(s) | 601, 1086 |
| SfcI | CTRYAG | 2 cut(s) | 171, 774 |
| SmlI | CTYRAG | 2 cut(s) | 781, 1036 |
| SmoI | CTYRAG | 2 cut(s) | 781, 1036 |
| Sse9I | AATT | 9 cut(s) | 43, 177, 374, 545, 669, 747, 1015, 1027, 1072 |
| SseBI | AGGCCT | 1 cut(s) | 35 |
| SsiI | CCGC | 2 cut(s) | 19, 762 |
| SspMI | CTAG | 5 cut(s) | 8, 414, 584, 666, 845 |
| StuI | AGGCCT | 1 cut(s) | 35 |
| StyI | CCWWGG | 2 cut(s) | 325, 1057 |
| TaaI | ACNGT | 4 cut(s) | 73, 500, 859, 1105 |
| TaiI | ACGT | 2 cut(s) | 103, 402 |
| TaqI | TCGA | 3 cut(s) | 495, 816, 1076 |
| TasI | AATT | 9 cut(s) | 43, 177, 374, 545, 669, 747, 1015, 1027, 1072 |
| TatI | WGTACW | 1 cut(s) | 995 |
| TauI | GCSGC | 1 cut(s) | 764 |
| TfiI | GAWTC | 2 cut(s) | 92, 895 |
| Tru1I | TTAA | 2 cut(s) | 431, 573 |
| Tru9I | TTAA | 2 cut(s) | 431, 573 |
| TscAI | CASTG | 3 cut(s) | 76, 487, 637 |
| TseFI | GTSAC | 2 cut(s) | 829, 840 |
| TseI | GCWGC | 6 cut(s) | 123, 170, 173, 682, 773, 1066 |
| Tsp45I | GTSAC | 2 cut(s) | 829, 840 |
| TspDTI | ATGAA | 3 cut(s) | 68, 456, 871 |
| TspGWI | ACGGA | 1 cut(s) | 955 |
| TspRI | CASTG | 3 cut(s) | 76, 487, 637 |
| XapI | RAATTY | 3 cut(s) | 545, 669, 1072 |
| XbaI | TCTAGA | 3 cut(s) | 413, 583, 665 |
| XceI | RCATGY | 1 cut(s) | 444 |
| XmiI | GTMKAC | 1 cut(s) | 864 |
| XspI | CTAG | 5 cut(s) | 8, 414, 584, 666, 845 |