Heat shock cognate 70 kDa
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccB1I | GGYRCC | 1 cut(s) | 677 |
| AccBSI | CCGCTC | 1 cut(s) | 641 |
| AciI | CCGC | 2 cut(s) | 641, 689 |
| AclWI | GGATC | 1 cut(s) | 322 |
| AcuI | CTGAAG | 1 cut(s) | 705 |
| AcyI | GRCGYC | 2 cut(s) | 654, 678 |
| AfaI | GTAC | 1 cut(s) | 280 |
| AfiI | CCNNNNNNNGG | 3 cut(s) | 583, 663, 664 |
| AflIII | ACRYGT | 1 cut(s) | 564 |
| AgsI | TTSAA | 7 cut(s) | 124, 188, 230, 307, 620, 746, 755 |
| AjnI | CCWGG | 2 cut(s) | 484, 503 |
| AluBI | AGCT | 4 cut(s) | 136, 182, 191, 697 |
| AluI | AGCT | 4 cut(s) | 136, 182, 191, 697 |
| Alw26I | GTCTC | 1 cut(s) | 556 |
| AlwI | GGATC | 1 cut(s) | 322 |
| Ama87I | CYCGRG | 1 cut(s) | 663 |
| AoxI | GGCC | 1 cut(s) | 574 |
| ApeKI | GCWGC | 5 cut(s) | 179, 191, 717, 720, 809 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 119 |
| AspLEI | GCGC | 1 cut(s) | 680 |
| AspS9I | GGNCC | 1 cut(s) | 488 |
| AsuC2I | CCSGG | 2 cut(s) | 664, 665 |
| AsuHPI | GGTGA | 1 cut(s) | 434 |
| AvaI | CYCGRG | 1 cut(s) | 663 |
| AvaII | GGWCC | 1 cut(s) | 488 |
| BanI | GGYRCC | 1 cut(s) | 677 |
| BanII | GRGCYC | 1 cut(s) | 735 |
| BbvCI | CCTCAGC | 1 cut(s) | 558 |
| BbvI | GCAGC | 5 cut(s) | 178, 191, 704, 707, 796 |
| BccI | CCATC | 1 cut(s) | 359 |
| BciT130I | CCWGG | 2 cut(s) | 486, 505 |
| BciVI | GTATCC | 1 cut(s) | 551 |
| BcnI | CCSGG | 2 cut(s) | 664, 665 |
| BcoDI | GTCTC | 1 cut(s) | 556 |
| BfaI | CTAG | 1 cut(s) | 84 |
| BfmI | CTRYAG | 2 cut(s) | 110, 192 |
| BfoI | RGCGCY | 1 cut(s) | 681 |
| BfuI | GTATCC | 1 cut(s) | 551 |
| BglII | AGATCT | 1 cut(s) | 670 |
| BisI | GCNGC | 6 cut(s) | 180, 192, 690, 718, 721, 810 |
| BlsI | GCNGC | 6 cut(s) | 181, 193, 691, 719, 722, 811 |
| BmcAI | AGTACT | 1 cut(s) | 280 |
| Bme1390I | CCNGG | 4 cut(s) | 486, 505, 664, 665 |
| Bme18I | GGWCC | 1 cut(s) | 488 |
| BmeT110I | CYCGRG | 1 cut(s) | 663 |
| BmgT120I | GGNCC | 1 cut(s) | 488 |
| BmiI | GGNNCC | 2 cut(s) | 6, 679 |
| BmrFI | CCNGG | 4 cut(s) | 486, 505, 664, 665 |
| BmsI | GCATC | 2 cut(s) | 19, 520 |
| BpmI | CTGGAG | 1 cut(s) | 468 |
| Bpu10I | CCTNAGC | 1 cut(s) | 558 |
| BpuEI | CTTGAG | 1 cut(s) | 725 |
| BpuMI | CCSGG | 2 cut(s) | 664, 665 |
| BsaBI | GATNNNNATC | 1 cut(s) | 593 |
| BsaHI | GRCGYC | 2 cut(s) | 654, 678 |
| BsaJI | CCNNGG | 3 cut(s) | 93, 663, 712 |
| Bsc4I | CCNNNNNNNGG | 3 cut(s) | 583, 663, 664 |
| Bse8I | GATNNNNATC | 1 cut(s) | 593 |
| BseBI | CCWGG | 2 cut(s) | 486, 505 |
| BseDI | CCNNGG | 3 cut(s) | 93, 663, 712 |
| BseGI | GGATG | 2 cut(s) | 34, 649 |
| BseJI | GATNNNNATC | 1 cut(s) | 593 |
| BseLI | CCNNNNNNNGG | 3 cut(s) | 583, 663, 664 |
| BseMII | CTCAG | 4 cut(s) | 411, 536, 549, 712 |
| BseRI | GAGGAG | 3 cut(s) | 797, 800, 803 |
| BseXI | GCAGC | 5 cut(s) | 178, 191, 704, 707, 796 |
| BsgI | GTGCAG | 1 cut(s) | 198 |
| BshFI | GGCC | 1 cut(s) | 576 |
| BshNI | GGYRCC | 1 cut(s) | 677 |
| BsiHKCI | CYCGRG | 1 cut(s) | 663 |
| BsiSI | CCGG | 1 cut(s) | 664 |
| BslI | CCNNNNNNNGG | 3 cut(s) | 583, 663, 664 |
| BsmAI | GTCTC | 1 cut(s) | 556 |
| BsmI | GAATGC | 2 cut(s) | 477, 637 |
| BsnI | GGCC | 1 cut(s) | 576 |
| BsoBI | CYCGRG | 1 cut(s) | 663 |
| Bsp1286I | GDGCHC | 1 cut(s) | 735 |
| Bsp143I | GATC | 2 cut(s) | 327, 670 |
| Bsp19I | CCATGG | 1 cut(s) | 712 |
| BspACI | CCGC | 2 cut(s) | 641, 689 |
| BspANI | GGCC | 1 cut(s) | 576 |
| BspCNI | CTCAG | 4 cut(s) | 410, 537, 550, 711 |
| BspHI | TCATGA | 2 cut(s) | 315, 382 |
| BspLI | GGNNCC | 2 cut(s) | 6, 679 |
| BspMAI | CTGCAG | 1 cut(s) | 196 |
| BspPI | GGATC | 1 cut(s) | 322 |
| BspQI | GCTCTTC | 1 cut(s) | 85 |
| BspT107I | GGYRCC | 1 cut(s) | 677 |
| BsrBI | CCGCTC | 1 cut(s) | 641 |
| BssECI | CCNNGG | 3 cut(s) | 93, 663, 712 |
| BssMI | GATC | 2 cut(s) | 327, 670 |
| BssNI | GRCGYC | 2 cut(s) | 654, 678 |
| BssT1I | CCWWGG | 2 cut(s) | 93, 712 |
| Bst2UI | CCWGG | 2 cut(s) | 486, 505 |
| Bst4CI | ACNGT | 1 cut(s) | 602 |
| Bst6I | CTCTTC | 3 cut(s) | 85, 384, 762 |
| BstACI | GRCGYC | 2 cut(s) | 654, 678 |
| BstAPI | GCANNNNNTGC | 2 cut(s) | 106, 176 |
| BstC8I | GCNNGC | 1 cut(s) | 725 |
| BstDEI | CTNAG | 5 cut(s) | 397, 545, 558, 698, 813 |
| BstDSI | CCRYGG | 1 cut(s) | 712 |
| BstF5I | GGATG | 2 cut(s) | 34, 649 |
| BstH2I | RGCGCY | 1 cut(s) | 681 |
| BstHHI | GCGC | 1 cut(s) | 680 |
| BstKTI | GATC | 2 cut(s) | 330, 673 |
| BstMAI | GTCTC | 1 cut(s) | 556 |
| BstMBI | GATC | 2 cut(s) | 327, 670 |
| BstMWI | GCNNNNNNNGC | 4 cut(s) | 106, 176, 188, 686 |
| BstNI | CCWGG | 2 cut(s) | 486, 505 |
| BstNSI | RCATGY | 1 cut(s) | 568 |
| BstSCI | CCNGG | 4 cut(s) | 484, 503, 662, 663 |
| BstSFI | CTRYAG | 2 cut(s) | 110, 192 |
| BstV1I | GCAGC | 5 cut(s) | 178, 191, 704, 707, 796 |
| BstX2I | RGATCY | 1 cut(s) | 670 |
| BstYI | RGATCY | 1 cut(s) | 670 |
| BsuI | GTATCC | 1 cut(s) | 551 |
| BsuRI | GGCC | 1 cut(s) | 576 |
| BtgI | CCRYGG | 1 cut(s) | 712 |
| BtsCI | GGATG | 2 cut(s) | 34, 649 |
| BtsIMutI | CAGTG | 1 cut(s) | 681 |
| Cac8I | GCNNGC | 1 cut(s) | 725 |
| CciI | TCATGA | 2 cut(s) | 315, 382 |
| CfoI | GCGC | 1 cut(s) | 680 |
| Cfr13I | GGNCC | 1 cut(s) | 488 |
| Cfr9I | CCCGGG | 1 cut(s) | 663 |
| CseI | GACGC | 1 cut(s) | 662 |
| Csp6I | GTAC | 1 cut(s) | 279 |
| CviAII | CATG | 8 cut(s) | 56, 218, 244, 316, 383, 473, 565, 713 |
| CviQI | GTAC | 1 cut(s) | 279 |
| DdeI | CTNAG | 5 cut(s) | 397, 545, 558, 698, 813 |
| DinI | GGCGCC | 1 cut(s) | 679 |
| DpnI | GATC | 2 cut(s) | 329, 672 |
| DpnII | GATC | 2 cut(s) | 327, 670 |
| Eam1104I | CTCTTC | 3 cut(s) | 85, 384, 762 |
| EarI | CTCTTC | 3 cut(s) | 85, 384, 762 |
| Eco130I | CCWWGG | 2 cut(s) | 93, 712 |
| Eco24I | GRGCYC | 1 cut(s) | 735 |
| Eco32I | GATATC | 1 cut(s) | 250 |
| Eco47I | GGWCC | 1 cut(s) | 488 |
| Eco57I | CTGAAG | 1 cut(s) | 705 |
| Eco88I | CYCGRG | 1 cut(s) | 663 |
| EcoO109I | RGGNCCY | 1 cut(s) | 488 |
| EcoRII | CCWGG | 2 cut(s) | 484, 503 |
| EcoRV | GATATC | 1 cut(s) | 250 |
| EcoT14I | CCWWGG | 2 cut(s) | 93, 712 |
| EcoT38I | GRGCYC | 1 cut(s) | 735 |
| EgeI | GGCGCC | 1 cut(s) | 679 |
| EheI | GGCGCC | 1 cut(s) | 679 |
| ErhI | CCWWGG | 2 cut(s) | 93, 712 |
| FaeI | CATG | 8 cut(s) | 59, 221, 247, 319, 386, 476, 568, 716 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 325, 357 |
| FatI | CATG | 8 cut(s) | 55, 217, 243, 315, 382, 472, 564, 712 |
| FauNDI | CATATG | 1 cut(s) | 172 |
| Fnu4HI | GCNGC | 6 cut(s) | 180, 192, 690, 718, 721, 810 |
| FokI | GGATG | 2 cut(s) | 41, 656 |
| FriOI | GRGCYC | 1 cut(s) | 735 |
| Fsp4HI | GCNGC | 6 cut(s) | 180, 192, 690, 718, 721, 810 |
| FspBI | CTAG | 1 cut(s) | 84 |
| GlaI | GCGC | 1 cut(s) | 679 |
| GluI | GCNGC | 6 cut(s) | 180, 192, 690, 718, 721, 810 |
| GsuI | CTGGAG | 1 cut(s) | 468 |
| HaeII | RGCGCY | 1 cut(s) | 681 |
| HaeIII | GGCC | 1 cut(s) | 576 |
| HapII | CCGG | 1 cut(s) | 664 |
| HgaI | GACGC | 1 cut(s) | 662 |
| HhaI | GCGC | 1 cut(s) | 680 |
| Hin1I | GRCGYC | 2 cut(s) | 654, 678 |
| Hin1II | CATG | 8 cut(s) | 59, 221, 247, 319, 386, 476, 568, 716 |
| Hin6I | GCGC | 1 cut(s) | 678 |
| HinP1I | GCGC | 1 cut(s) | 678 |
| HinfI | GANTC | 3 cut(s) | 87, 589, 647 |
| HpaII | CCGG | 1 cut(s) | 664 |
| HphI | GGTGA | 1 cut(s) | 434 |
| Hpy166II | GTNNAC | 1 cut(s) | 570 |
| Hpy188I | TCNGA | 1 cut(s) | 652 |
| Hpy188III | TCNNGA | 9 cut(s) | 84, 155, 199, 230, 316, 355, 383, 544, 704 |
| Hpy8I | GTNNAC | 1 cut(s) | 570 |
| HpyAV | CCTTC | 4 cut(s) | 124, 577, 614, 772 |
| HpyCH4III | ACNGT | 1 cut(s) | 602 |
| HpyCH4IV | ACGT | 1 cut(s) | 42 |
| HpyCH4V | TGCA | 6 cut(s) | 100, 141, 179, 194, 217, 533 |
| HpyF10VI | GCNNNNNNNGC | 4 cut(s) | 106, 176, 188, 686 |
| HpyF3I | CTNAG | 5 cut(s) | 397, 545, 558, 698, 813 |
| HpySE526I | ACGT | 1 cut(s) | 42 |
| Hsp92I | GRCGYC | 2 cut(s) | 654, 678 |
| Hsp92II | CATG | 8 cut(s) | 59, 221, 247, 319, 386, 476, 568, 716 |
| HspAI | GCGC | 1 cut(s) | 678 |
| KasI | GGCGCC | 1 cut(s) | 677 |
| Kzo9I | GATC | 2 cut(s) | 327, 670 |
| LguI | GCTCTTC | 1 cut(s) | 85 |
| LmnI | GCTCC | 2 cut(s) | 4, 133 |
| Lsp1109I | GCAGC | 5 cut(s) | 178, 191, 704, 707, 796 |
| LweI | GCATC | 2 cut(s) | 19, 520 |
| MaeI | CTAG | 1 cut(s) | 84 |
| MaeII | ACGT | 1 cut(s) | 42 |
| MalI | GATC | 2 cut(s) | 329, 672 |
| MbiI | CCGCTC | 1 cut(s) | 641 |
| MboI | GATC | 2 cut(s) | 327, 670 |
| MboII | GAAGA | 8 cut(s) | 72, 370, 371, 773, 776, 779, 807, 810 |
| MflI | RGATCY | 1 cut(s) | 670 |
| MhlI | GDGCHC | 1 cut(s) | 735 |
| MluCI | AATT | 1 cut(s) | 311 |
| Mly113I | GGCGCC | 1 cut(s) | 678 |
| MroXI | GAANNNNTTC | 1 cut(s) | 119 |
| MslI | CAYNNNNRTG | 2 cut(s) | 242, 320 |
| MspA1I | CMGCKG | 1 cut(s) | 182 |
| MspI | CCGG | 1 cut(s) | 664 |
| MspR9I | CCNGG | 4 cut(s) | 486, 505, 664, 665 |
| Mva1269I | GAATGC | 2 cut(s) | 477, 637 |
| MvaI | CCWGG | 2 cut(s) | 486, 505 |
| MwoI | GCNNNNNNNGC | 4 cut(s) | 106, 176, 188, 686 |
| NarI | GGCGCC | 1 cut(s) | 678 |
| NciI | CCSGG | 2 cut(s) | 664, 665 |
| NcoI | CCATGG | 1 cut(s) | 712 |
| NdeI | CATATG | 1 cut(s) | 172 |
| NdeII | GATC | 2 cut(s) | 327, 670 |
| NlaIII | CATG | 8 cut(s) | 59, 221, 247, 319, 386, 476, 568, 716 |
| NlaIV | GGNNCC | 2 cut(s) | 6, 679 |
| NmeAIII | GCCGAG | 1 cut(s) | 654 |
| NspI | RCATGY | 1 cut(s) | 568 |
| PagI | TCATGA | 2 cut(s) | 315, 382 |
| PciI | ACATGT | 1 cut(s) | 564 |
| PciSI | GCTCTTC | 1 cut(s) | 85 |
| PctI | GAATGC | 2 cut(s) | 477, 637 |
| PdmI | GAANNNNTTC | 1 cut(s) | 119 |
| PfeI | GAWTC | 3 cut(s) | 87, 589, 647 |
| PkrI | GCNGC | 6 cut(s) | 181, 193, 691, 719, 722, 811 |
| PluTI | GGCGCC | 1 cut(s) | 681 |
| PpuMI | RGGWCCY | 1 cut(s) | 488 |
| PscI | ACATGT | 1 cut(s) | 564 |
| Psp5II | RGGWCCY | 1 cut(s) | 488 |
| Psp6I | CCWGG | 2 cut(s) | 484, 503 |
| PspGI | CCWGG | 2 cut(s) | 484, 503 |
| PspN4I | GGNNCC | 2 cut(s) | 6, 679 |
| PspPI | GGNCC | 1 cut(s) | 488 |
| PspPPI | RGGWCCY | 1 cut(s) | 488 |
| PstI | CTGCAG | 1 cut(s) | 196 |
| PsuI | RGATCY | 1 cut(s) | 670 |
| PvuII | CAGCTG | 1 cut(s) | 182 |
| RsaI | GTAC | 1 cut(s) | 280 |
| RsaNI | GTAC | 1 cut(s) | 279 |
| RseI | CAYNNNNRTG | 2 cut(s) | 242, 320 |
| SapI | GCTCTTC | 1 cut(s) | 85 |
| SatI | GCNGC | 6 cut(s) | 180, 192, 690, 718, 721, 810 |
| Sau3AI | GATC | 2 cut(s) | 327, 670 |
| Sau96I | GGNCC | 1 cut(s) | 488 |
| ScaI | AGTACT | 1 cut(s) | 280 |
| ScrFI | CCNGG | 4 cut(s) | 486, 505, 664, 665 |
| SduI | GDGCHC | 1 cut(s) | 735 |
| SfaNI | GCATC | 2 cut(s) | 19, 520 |
| SfcI | CTRYAG | 2 cut(s) | 110, 192 |
| SfoI | GGCGCC | 1 cut(s) | 679 |
| SinI | GGWCC | 1 cut(s) | 488 |
| SmaI | CCCGGG | 1 cut(s) | 665 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 242, 320 |
| SmlI | CTYRAG | 1 cut(s) | 704 |
| SmoI | CTYRAG | 1 cut(s) | 704 |
| Sse9I | AATT | 1 cut(s) | 311 |
| SsiI | CCGC | 2 cut(s) | 641, 689 |
| SspDI | GGCGCC | 1 cut(s) | 677 |
| SspMI | CTAG | 1 cut(s) | 84 |
| StyD4I | CCNGG | 4 cut(s) | 484, 503, 662, 663 |
| StyI | CCWWGG | 2 cut(s) | 93, 712 |
| TaaI | ACNGT | 1 cut(s) | 602 |
| TaiI | ACGT | 1 cut(s) | 45 |
| TasI | AATT | 1 cut(s) | 311 |
| TatI | WGTACW | 1 cut(s) | 278 |
| TauI | GCSGC | 1 cut(s) | 692 |
| TfiI | GAWTC | 3 cut(s) | 87, 589, 647 |
| TscAI | CASTG | 1 cut(s) | 688 |
| TseI | GCWGC | 5 cut(s) | 179, 191, 717, 720, 809 |
| TspDTI | ATGAA | 5 cut(s) | 304, 332, 371, 461, 660 |
| TspMI | CCCGGG | 1 cut(s) | 663 |
| TspRI | CASTG | 1 cut(s) | 688 |
| VpaK11BI | GGWCC | 1 cut(s) | 488 |
| XbaI | TCTAGA | 1 cut(s) | 83 |
| XceI | RCATGY | 1 cut(s) | 568 |
| XmaI | CCCGGG | 1 cut(s) | 663 |
| XmnI | GAANNNNTTC | 1 cut(s) | 119 |
| XspI | CTAG | 1 cut(s) | 84 |
| ZrmI | AGTACT | 1 cut(s) | 280 |