Heat shock cognate 70 kDa
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AciI | CCGC | 2 cut(s) | 162, 262 |
| AcoI | YGGCCR | 1 cut(s) | 46 |
| AcsI | RAATTY | 2 cut(s) | 517, 688 |
| AcuI | CTGAAG | 2 cut(s) | 149, 213 |
| AfaI | GTAC | 2 cut(s) | 181, 505 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 199 |
| AgsI | TTSAA | 7 cut(s) | 54, 64, 337, 439, 634, 694, 717 |
| AjiI | CACGTC | 1 cut(s) | 543 |
| AjnI | CCWGG | 2 cut(s) | 245, 556 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 520, 552 |
| AleI | CACNNNNGTG | 1 cut(s) | 470 |
| AloI | GAACNNNNNNTCC | 2 cut(s) | 409, 441 |
| AluBI | AGCT | 2 cut(s) | 30, 313 |
| AluI | AGCT | 2 cut(s) | 30, 313 |
| Alw26I | GTCTC | 3 cut(s) | 411, 452, 597 |
| AoxI | GGCC | 3 cut(s) | 46, 276, 330 |
| ApeKI | GCWGC | 4 cut(s) | 124, 240, 313, 316 |
| ApoI | RAATTY | 2 cut(s) | 517, 688 |
| AseI | ATTAAT | 1 cut(s) | 299 |
| AspA2I | CCTAGG | 1 cut(s) | 468 |
| AspLEI | GCGC | 1 cut(s) | 245 |
| AsuC2I | CCSGG | 1 cut(s) | 429 |
| AsuHPI | GGTGA | 6 cut(s) | 82, 204, 277, 297, 457, 487 |
| AvrII | CCTAGG | 1 cut(s) | 468 |
| BalI | TGGCCA | 1 cut(s) | 48 |
| BanII | GRGCYC | 1 cut(s) | 564 |
| BauI | CACGAG | 2 cut(s) | 360, 680 |
| BbvI | GCAGC | 4 cut(s) | 111, 252, 300, 328 |
| BccI | CCATC | 1 cut(s) | 366 |
| BciT130I | CCWGG | 2 cut(s) | 247, 558 |
| BcnI | CCSGG | 1 cut(s) | 429 |
| BcoDI | GTCTC | 3 cut(s) | 411, 452, 597 |
| BfaI | CTAG | 1 cut(s) | 469 |
| BfmI | CTRYAG | 1 cut(s) | 314 |
| BfoI | RGCGCY | 1 cut(s) | 246 |
| BisI | GCNGC | 4 cut(s) | 125, 241, 314, 317 |
| BlnI | CCTAGG | 1 cut(s) | 468 |
| BlpI | GCTNAGC | 1 cut(s) | 173 |
| BlsI | GCNGC | 4 cut(s) | 126, 242, 315, 318 |
| BmcAI | AGTACT | 1 cut(s) | 505 |
| Bme1390I | CCNGG | 3 cut(s) | 247, 429, 558 |
| BmgBI | CACGTC | 1 cut(s) | 543 |
| BmiI | GGNNCC | 1 cut(s) | 731 |
| BmrFI | CCNGG | 3 cut(s) | 247, 429, 558 |
| BmsI | GCATC | 2 cut(s) | 149, 278 |
| BpmI | CTGGAG | 1 cut(s) | 540 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 173 |
| BpuMI | CCSGG | 1 cut(s) | 429 |
| BsaBI | GATNNNNATC | 1 cut(s) | 665 |
| BsaJI | CCNNGG | 4 cut(s) | 143, 428, 468, 557 |
| BsaXI | ACNNNNNCTCC | 5 cut(s) | 27, 103, 133, 172, 202 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 199 |
| Bse3DI | GCAATG | 1 cut(s) | 167 |
| Bse8I | GATNNNNATC | 1 cut(s) | 665 |
| BseBI | CCWGG | 2 cut(s) | 247, 558 |
| BseDI | CCNNGG | 4 cut(s) | 143, 428, 468, 557 |
| BseJI | GATNNNNATC | 1 cut(s) | 665 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 199 |
| BseMI | GCAATG | 1 cut(s) | 167 |
| BseMII | CTCAG | 1 cut(s) | 164 |
| BseRI | GAGGAG | 1 cut(s) | 129 |
| BseXI | GCAGC | 4 cut(s) | 111, 252, 300, 328 |
| BsgI | GTGCAG | 1 cut(s) | 224 |
| Bsh1285I | CGRYCG | 1 cut(s) | 455 |
| BshFI | GGCC | 3 cut(s) | 48, 278, 332 |
| BsiEI | CGRYCG | 1 cut(s) | 455 |
| BsiSI | CCGG | 1 cut(s) | 428 |
| BslFI | GGGAC | 2 cut(s) | 178, 202 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 199 |
| BsmAI | GTCTC | 3 cut(s) | 411, 452, 597 |
| BsmFI | GGGAC | 2 cut(s) | 178, 202 |
| BsnI | GGCC | 3 cut(s) | 48, 278, 332 |
| Bsp1286I | GDGCHC | 1 cut(s) | 564 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 173 |
| Bsp19I | CCATGG | 1 cut(s) | 143 |
| BspACI | CCGC | 2 cut(s) | 162, 262 |
| BspANI | GGCC | 3 cut(s) | 48, 278, 332 |
| BspCNI | CTCAG | 1 cut(s) | 165 |
| BspHI | TCATGA | 1 cut(s) | 99 |
| BspLI | GGNNCC | 1 cut(s) | 731 |
| BspMAI | CTGCAG | 1 cut(s) | 318 |
| BsrDI | GCAATG | 1 cut(s) | 167 |
| BssECI | CCNNGG | 4 cut(s) | 143, 428, 468, 557 |
| BssSI | CACGAG | 2 cut(s) | 360, 680 |
| BssT1I | CCWWGG | 2 cut(s) | 143, 468 |
| Bst2BI | CACGAG | 2 cut(s) | 360, 680 |
| Bst2UI | CCWGG | 2 cut(s) | 247, 558 |
| Bst4CI | ACNGT | 5 cut(s) | 119, 216, 453, 508, 643 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 322 |
| BstC8I | GCNNGC | 2 cut(s) | 276, 348 |
| BstDEI | CTNAG | 1 cut(s) | 173 |
| BstDSI | CCRYGG | 1 cut(s) | 143 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 197 |
| BstH2I | RGCGCY | 1 cut(s) | 246 |
| BstHHI | GCGC | 1 cut(s) | 245 |
| BstMAI | GTCTC | 3 cut(s) | 411, 452, 597 |
| BstMCI | CGRYCG | 1 cut(s) | 455 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 322, 356 |
| BstNI | CCWGG | 2 cut(s) | 247, 558 |
| BstSCI | CCNGG | 3 cut(s) | 245, 427, 556 |
| BstSFI | CTRYAG | 1 cut(s) | 314 |
| BstV1I | GCAGC | 4 cut(s) | 111, 252, 300, 328 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 150 |
| BsuRI | GGCC | 3 cut(s) | 48, 278, 332 |
| BtgI | CCRYGG | 1 cut(s) | 143 |
| BtrI | CACGTC | 1 cut(s) | 543 |
| BtsI | GCAGTG | 1 cut(s) | 409 |
| BtsIMutI | CAGTG | 3 cut(s) | 18, 237, 409 |
| Cac8I | GCNNGC | 2 cut(s) | 276, 348 |
| CciI | TCATGA | 1 cut(s) | 99 |
| CfoI | GCGC | 1 cut(s) | 245 |
| Csp6I | GTAC | 2 cut(s) | 180, 504 |
| CviAII | CATG | 3 cut(s) | 100, 144, 705 |
| CviQI | GTAC | 2 cut(s) | 180, 504 |
| DdeI | CTNAG | 1 cut(s) | 173 |
| EaeI | YGGCCR | 1 cut(s) | 46 |
| Eco130I | CCWWGG | 2 cut(s) | 143, 468 |
| Eco147I | AGGCCT | 1 cut(s) | 278 |
| Eco24I | GRGCYC | 1 cut(s) | 564 |
| Eco57I | CTGAAG | 2 cut(s) | 149, 213 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 197 |
| EcoRII | CCWGG | 2 cut(s) | 245, 556 |
| EcoT14I | CCWWGG | 2 cut(s) | 143, 468 |
| EcoT38I | GRGCYC | 1 cut(s) | 564 |
| ErhI | CCWWGG | 2 cut(s) | 143, 468 |
| FaeI | CATG | 3 cut(s) | 103, 147, 708 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 567, 599 |
| FaqI | GGGAC | 2 cut(s) | 178, 202 |
| FatI | CATG | 3 cut(s) | 99, 143, 704 |
| FauI | CCCGC | 2 cut(s) | 155, 255 |
| Fnu4HI | GCNGC | 4 cut(s) | 125, 241, 314, 317 |
| FriOI | GRGCYC | 1 cut(s) | 564 |
| Fsp4HI | GCNGC | 4 cut(s) | 125, 241, 314, 317 |
| FspBI | CTAG | 1 cut(s) | 469 |
| GlaI | GCGC | 1 cut(s) | 244 |
| GluI | GCNGC | 4 cut(s) | 125, 241, 314, 317 |
| GsuI | CTGGAG | 1 cut(s) | 540 |
| HaeII | RGCGCY | 1 cut(s) | 246 |
| HaeIII | GGCC | 3 cut(s) | 48, 278, 332 |
| HapII | CCGG | 1 cut(s) | 428 |
| HhaI | GCGC | 1 cut(s) | 245 |
| Hin1II | CATG | 3 cut(s) | 103, 147, 708 |
| Hin6I | GCGC | 1 cut(s) | 243 |
| HinP1I | GCGC | 1 cut(s) | 243 |
| HinfI | GANTC | 4 cut(s) | 8, 187, 235, 605 |
| HpaII | CCGG | 1 cut(s) | 428 |
| HphI | GGTGA | 6 cut(s) | 82, 204, 277, 297, 457, 487 |
| Hpy188I | TCNGA | 1 cut(s) | 457 |
| Hpy188III | TCNNGA | 2 cut(s) | 100, 109 |
| HpyAV | CCTTC | 3 cut(s) | 58, 193, 585 |
| HpyCH4III | ACNGT | 5 cut(s) | 119, 216, 453, 508, 643 |
| HpyCH4IV | ACGT | 1 cut(s) | 542 |
| HpyCH4V | TGCA | 3 cut(s) | 205, 274, 316 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 322, 356 |
| HpyF3I | CTNAG | 1 cut(s) | 173 |
| HpySE526I | ACGT | 1 cut(s) | 542 |
| Hsp92II | CATG | 3 cut(s) | 103, 147, 708 |
| HspAI | GCGC | 1 cut(s) | 243 |
| LmnI | GCTCC | 1 cut(s) | 729 |
| Lsp1109I | GCAGC | 4 cut(s) | 111, 252, 300, 328 |
| LweI | GCATC | 2 cut(s) | 149, 278 |
| MaeI | CTAG | 1 cut(s) | 469 |
| MaeII | ACGT | 1 cut(s) | 542 |
| MaeIII | GTNAC | 3 cut(s) | 13, 93, 550 |
| MboII | GAAGA | 5 cut(s) | 142, 490, 537, 611, 705 |
| MfeI | CAATTG | 1 cut(s) | 320 |
| MhlI | GDGCHC | 1 cut(s) | 564 |
| MlsI | TGGCCA | 1 cut(s) | 48 |
| MluCI | AATT | 6 cut(s) | 269, 296, 320, 517, 534, 688 |
| MluNI | TGGCCA | 1 cut(s) | 48 |
| MlyI | GAGTC | 2 cut(s) | 181, 599 |
| MmeI | TCCRAC | 1 cut(s) | 435 |
| MnlI | CCTC | 4 cut(s) | 96, 150, 335, 507 |
| Mox20I | TGGCCA | 1 cut(s) | 48 |
| MscI | TGGCCA | 1 cut(s) | 48 |
| MseI | TTAA | 4 cut(s) | 299, 500, 521, 574 |
| MslI | CAYNNNNRTG | 1 cut(s) | 470 |
| Msp20I | TGGCCA | 1 cut(s) | 48 |
| MspA1I | CMGCKG | 1 cut(s) | 313 |
| MspI | CCGG | 1 cut(s) | 428 |
| MspR9I | CCNGG | 3 cut(s) | 247, 429, 558 |
| MunI | CAATTG | 1 cut(s) | 320 |
| MvaI | CCWGG | 2 cut(s) | 247, 558 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 322, 356 |
| NciI | CCSGG | 1 cut(s) | 429 |
| NcoI | CCATGG | 1 cut(s) | 143 |
| NlaIII | CATG | 3 cut(s) | 103, 147, 708 |
| NlaIV | GGNNCC | 1 cut(s) | 731 |
| NmuCI | GTSAC | 1 cut(s) | 13 |
| OliI | CACNNNNGTG | 1 cut(s) | 470 |
| PagI | TCATGA | 1 cut(s) | 99 |
| PceI | AGGCCT | 1 cut(s) | 278 |
| PfeI | GAWTC | 2 cut(s) | 8, 235 |
| PkrI | GCNGC | 4 cut(s) | 126, 242, 315, 318 |
| PleI | GAGTC | 2 cut(s) | 181, 599 |
| PpsI | GAGTC | 2 cut(s) | 181, 599 |
| PshBI | ATTAAT | 1 cut(s) | 299 |
| Psp6I | CCWGG | 2 cut(s) | 245, 556 |
| PspGI | CCWGG | 2 cut(s) | 245, 556 |
| PspN4I | GGNNCC | 1 cut(s) | 731 |
| PstI | CTGCAG | 1 cut(s) | 318 |
| PvuII | CAGCTG | 1 cut(s) | 313 |
| RsaI | GTAC | 2 cut(s) | 181, 505 |
| RsaNI | GTAC | 2 cut(s) | 180, 504 |
| RseI | CAYNNNNRTG | 1 cut(s) | 470 |
| SaqAI | TTAA | 4 cut(s) | 299, 500, 521, 574 |
| SatI | GCNGC | 4 cut(s) | 125, 241, 314, 317 |
| ScaI | AGTACT | 1 cut(s) | 505 |
| SchI | GAGTC | 2 cut(s) | 181, 599 |
| ScrFI | CCNGG | 3 cut(s) | 247, 429, 558 |
| SduI | GDGCHC | 1 cut(s) | 564 |
| SfaNI | GCATC | 2 cut(s) | 149, 278 |
| SfcI | CTRYAG | 1 cut(s) | 314 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 470 |
| Sse9I | AATT | 6 cut(s) | 269, 296, 320, 517, 534, 688 |
| SseBI | AGGCCT | 1 cut(s) | 278 |
| SsiI | CCGC | 2 cut(s) | 162, 262 |
| SspMI | CTAG | 1 cut(s) | 469 |
| StuI | AGGCCT | 1 cut(s) | 278 |
| StyD4I | CCNGG | 3 cut(s) | 245, 427, 556 |
| StyI | CCWWGG | 2 cut(s) | 143, 468 |
| TaaI | ACNGT | 5 cut(s) | 119, 216, 453, 508, 643 |
| TaiI | ACGT | 1 cut(s) | 545 |
| TaqI | TCGA | 1 cut(s) | 638 |
| TasI | AATT | 6 cut(s) | 269, 296, 320, 517, 534, 688 |
| TatI | WGTACW | 1 cut(s) | 503 |
| TfiI | GAWTC | 2 cut(s) | 8, 235 |
| Tru1I | TTAA | 4 cut(s) | 299, 500, 521, 574 |
| Tru9I | TTAA | 4 cut(s) | 299, 500, 521, 574 |
| TscAI | CASTG | 3 cut(s) | 18, 237, 416 |
| TseFI | GTSAC | 1 cut(s) | 13 |
| TseI | GCWGC | 4 cut(s) | 124, 240, 313, 316 |
| Tsp45I | GTSAC | 1 cut(s) | 13 |
| TspDTI | ATGAA | 2 cut(s) | 52, 317 |
| TspRI | CASTG | 3 cut(s) | 18, 237, 416 |
| VspI | ATTAAT | 1 cut(s) | 299 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 197 |
| XapI | RAATTY | 2 cut(s) | 517, 688 |
| XmaJI | CCTAGG | 1 cut(s) | 468 |
| XspI | CTAG | 1 cut(s) | 469 |
| ZrmI | AGTACT | 1 cut(s) | 505 |