Heat shock cognate 70 kDa
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AarI | CACCTGC | 1 cut(s) | 1285 |
| Acc36I | ACCTGC | 2 cut(s) | 287, 1285 |
| AccB1I | GGYRCC | 1 cut(s) | 44 |
| AccII | CGCG | 1 cut(s) | 1371 |
| AciI | CCGC | 7 cut(s) | 171, 223, 394, 1139, 1369, 1666, 1881 |
| AclWI | GGATC | 1 cut(s) | 1252 |
| AcoI | YGGCCR | 1 cut(s) | 3 |
| AcsI | RAATTY | 1 cut(s) | 1391 |
| AcyI | GRCGYC | 1 cut(s) | 168 |
| AfaI | GTAC | 3 cut(s) | 1431, 1575, 1850 |
| AfiI | CCNNNNNNNGG | 3 cut(s) | 29, 1907, 1910 |
| AjnI | CCWGG | 4 cut(s) | 105, 180, 1324, 1851 |
| AleI | CACNNNNGTG | 1 cut(s) | 81 |
| Alw21I | GWGCWC | 3 cut(s) | 796, 1401, 1614 |
| Alw26I | GTCTC | 4 cut(s) | 569, 643, 1223, 1486 |
| AlwI | GGATC | 1 cut(s) | 1252 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 302 |
| AoxI | GGCC | 3 cut(s) | 3, 278, 408 |
| ApoI | RAATTY | 1 cut(s) | 1391 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 1896 |
| AspS9I | GGNCC | 5 cut(s) | 20, 279, 299, 1186, 1925 |
| AsuC2I | CCSGG | 1 cut(s) | 1112 |
| AvaII | GGWCC | 4 cut(s) | 20, 299, 1186, 1925 |
| BanI | GGYRCC | 1 cut(s) | 44 |
| BanII | GRGCYC | 3 cut(s) | 540, 796, 1401 |
| BbsI | GAAGAC | 3 cut(s) | 202, 1549, 1899 |
| Bbv12I | GWGCWC | 3 cut(s) | 796, 1401, 1614 |
| BbvCI | CCTCAGC | 2 cut(s) | 360, 1488 |
| BccI | CCATC | 5 cut(s) | 721, 988, 1532, 1564, 1757 |
| BceAI | ACGGC | 1 cut(s) | 109 |
| BcgI | CGANNNNNNTGC | 4 cut(s) | 62, 96, 1442, 1476 |
| BciT130I | CCWGG | 4 cut(s) | 107, 182, 1326, 1853 |
| BcnI | CCSGG | 1 cut(s) | 1112 |
| BcoDI | GTCTC | 4 cut(s) | 569, 643, 1223, 1486 |
| BfaI | CTAG | 2 cut(s) | 1041, 1199 |
| BfmI | CTRYAG | 1 cut(s) | 546 |
| BfuAI | ACCTGC | 2 cut(s) | 287, 1285 |
| BglII | AGATCT | 1 cut(s) | 366 |
| Bme1390I | CCNGG | 5 cut(s) | 107, 182, 1112, 1326, 1853 |
| Bme18I | GGWCC | 4 cut(s) | 20, 299, 1186, 1925 |
| BmgT120I | GGNCC | 5 cut(s) | 20, 279, 299, 1186, 1925 |
| BmiI | GGNNCC | 4 cut(s) | 21, 46, 963, 1927 |
| BmrFI | CCNGG | 5 cut(s) | 107, 182, 1112, 1326, 1853 |
| BpiI | GAAGAC | 3 cut(s) | 202, 1549, 1899 |
| BplI | GAGNNNNNCTC | 4 cut(s) | 353, 385, 857, 889 |
| BpmI | CTGGAG | 4 cut(s) | 516, 1257, 1398, 1664 |
| Bpu10I | CCTNAGC | 2 cut(s) | 360, 1488 |
| BpuEI | CTTGAG | 1 cut(s) | 1000 |
| BpuMI | CCSGG | 1 cut(s) | 1112 |
| BsaBI | GATNNNNATC | 1 cut(s) | 1523 |
| BsaHI | GRCGYC | 1 cut(s) | 168 |
| BsaI | GGTCTC | 1 cut(s) | 569 |
| BsaJI | CCNNGG | 7 cut(s) | 40, 106, 1218, 1262, 1421, 1551, 1852 |
| BsaXI | ACNNNNNCTCC | 4 cut(s) | 356, 386, 1283, 1313 |
| Bsc4I | CCNNNNNNNGG | 3 cut(s) | 29, 1907, 1910 |
| Bse1I | ACTGG | 4 cut(s) | 281, 582, 965, 1507 |
| Bse3DI | GCAATG | 4 cut(s) | 291, 501, 1177, 1836 |
| Bse8I | GATNNNNATC | 1 cut(s) | 1523 |
| BseBI | CCWGG | 4 cut(s) | 107, 182, 1326, 1853 |
| BseDI | CCNNGG | 7 cut(s) | 40, 106, 1218, 1262, 1421, 1551, 1852 |
| BseGI | GGATG | 7 cut(s) | 496, 732, 842, 1120, 1470, 1738, 1894 |
| BseJI | GATNNNNATC | 1 cut(s) | 1523 |
| BseLI | CCNNNNNNNGG | 3 cut(s) | 29, 1907, 1910 |
| BseMI | GCAATG | 4 cut(s) | 291, 501, 1177, 1836 |
| BseMII | CTCAG | 5 cut(s) | 141, 351, 866, 1479, 1575 |
| BseNI | ACTGG | 4 cut(s) | 281, 582, 965, 1507 |
| BseRI | GAGGAG | 5 cut(s) | 377, 720, 1200, 1622, 1802 |
| BsgI | GTGCAG | 1 cut(s) | 1202 |
| Bsh1236I | CGCG | 1 cut(s) | 1371 |
| Bsh1285I | CGRYCG | 2 cut(s) | 30, 82 |
| BshFI | GGCC | 3 cut(s) | 5, 280, 410 |
| BshNI | GGYRCC | 1 cut(s) | 44 |
| BsiEI | CGRYCG | 2 cut(s) | 30, 82 |
| BsiHKAI | GWGCWC | 3 cut(s) | 796, 1401, 1614 |
| BsiSI | CCGG | 2 cut(s) | 6, 1112 |
| BslFI | GGGAC | 2 cut(s) | 33, 1911 |
| BslI | CCNNNNNNNGG | 3 cut(s) | 29, 1907, 1910 |
| BsmAI | GTCTC | 4 cut(s) | 569, 643, 1223, 1486 |
| BsmFI | GGGAC | 2 cut(s) | 33, 1911 |
| BsmI | GAATGC | 2 cut(s) | 1407, 1642 |
| BsnI | GGCC | 3 cut(s) | 5, 280, 410 |
| Bso31I | GGTCTC | 1 cut(s) | 569 |
| Bsp1286I | GDGCHC | 4 cut(s) | 540, 796, 1401, 1614 |
| Bsp143I | GATC | 8 cut(s) | 27, 79, 90, 159, 366, 613, 1257, 1833 |
| BspACI | CCGC | 7 cut(s) | 171, 223, 394, 1139, 1369, 1666, 1881 |
| BspANI | GGCC | 3 cut(s) | 5, 280, 410 |
| BspCNI | CTCAG | 5 cut(s) | 142, 352, 865, 1480, 1576 |
| BspFNI | CGCG | 1 cut(s) | 1371 |
| BspLI | GGNNCC | 4 cut(s) | 21, 46, 963, 1927 |
| BspMAI | CTGCAG | 1 cut(s) | 550 |
| BspMI | ACCTGC | 2 cut(s) | 287, 1285 |
| BspPI | GGATC | 1 cut(s) | 1252 |
| BspQI | GCTCTTC | 1 cut(s) | 921 |
| BspT107I | GGYRCC | 1 cut(s) | 44 |
| BspTNI | GGTCTC | 1 cut(s) | 569 |
| BsrDI | GCAATG | 4 cut(s) | 291, 501, 1177, 1836 |
| BsrI | ACTGG | 4 cut(s) | 281, 582, 965, 1507 |
| BssECI | CCNNGG | 7 cut(s) | 40, 106, 1218, 1262, 1421, 1551, 1852 |
| BssMI | GATC | 8 cut(s) | 27, 79, 90, 159, 366, 613, 1257, 1833 |
| BssNI | GRCGYC | 1 cut(s) | 168 |
| BssT1I | CCWWGG | 4 cut(s) | 1218, 1262, 1421, 1551 |
| Bst2UI | CCWGG | 4 cut(s) | 107, 182, 1326, 1853 |
| Bst4CI | ACNGT | 7 cut(s) | 208, 256, 427, 448, 1252, 1276, 1444 |
| Bst6I | CTCTTC | 4 cut(s) | 336, 651, 824, 921 |
| BstACI | GRCGYC | 1 cut(s) | 168 |
| BstAPI | GCANNNNNTGC | 2 cut(s) | 302, 1063 |
| BstC8I | GCNNGC | 3 cut(s) | 453, 1146, 1879 |
| BstDEI | CTNAG | 8 cut(s) | 150, 174, 360, 797, 852, 1284, 1488, 1584 |
| BstF5I | GGATG | 7 cut(s) | 496, 732, 842, 1120, 1470, 1738, 1894 |
| BstFNI | CGCG | 1 cut(s) | 1371 |
| BstKTI | GATC | 8 cut(s) | 30, 82, 93, 162, 369, 616, 1260, 1836 |
| BstMAI | GTCTC | 4 cut(s) | 569, 643, 1223, 1486 |
| BstMBI | GATC | 8 cut(s) | 27, 79, 90, 159, 366, 613, 1257, 1833 |
| BstMCI | CGRYCG | 2 cut(s) | 30, 82 |
| BstMWI | GCNNNNNNNGC | 5 cut(s) | 302, 554, 1063, 1133, 1145 |
| BstNI | CCWGG | 4 cut(s) | 107, 182, 1326, 1853 |
| BstNSI | RCATGY | 1 cut(s) | 1666 |
| BstSCI | CCNGG | 5 cut(s) | 105, 180, 1110, 1324, 1851 |
| BstSFI | CTRYAG | 1 cut(s) | 546 |
| BstUI | CGCG | 1 cut(s) | 1371 |
| BstV2I | GAAGAC | 3 cut(s) | 202, 1549, 1899 |
| BstX2I | RGATCY | 1 cut(s) | 366 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 322 |
| BstYI | RGATCY | 1 cut(s) | 366 |
| BsuRI | GGCC | 3 cut(s) | 5, 280, 410 |
| BtgZI | GCGATG | 1 cut(s) | 79 |
| BtsCI | GGATG | 7 cut(s) | 496, 732, 842, 1120, 1470, 1738, 1894 |
| BtsI | GCAGTG | 4 cut(s) | 540, 846, 1012, 1921 |
| BtsIMutI | CAGTG | 8 cut(s) | 250, 274, 540, 589, 846, 1012, 1500, 1921 |
| BveI | ACCTGC | 2 cut(s) | 287, 1285 |
| Cac8I | GCNNGC | 3 cut(s) | 453, 1146, 1879 |
| CaiI | CAGNNNCTG | 1 cut(s) | 302 |
| Cfr13I | GGNCC | 5 cut(s) | 20, 279, 299, 1186, 1925 |
| CseI | GACGC | 3 cut(s) | 49, 176, 464 |
| CsiI | ACCWGGT | 2 cut(s) | 180, 1324 |
| Csp6I | GTAC | 3 cut(s) | 1430, 1574, 1849 |
| CviAII | CATG | 8 cut(s) | 190, 316, 937, 958, 1013, 1663, 1873, 1885 |
| CviQI | GTAC | 3 cut(s) | 1430, 1574, 1849 |
| DdeI | CTNAG | 8 cut(s) | 150, 174, 360, 797, 852, 1284, 1488, 1584 |
| DpnI | GATC | 8 cut(s) | 29, 81, 92, 161, 368, 615, 1259, 1835 |
| DpnII | GATC | 8 cut(s) | 27, 79, 90, 159, 366, 613, 1257, 1833 |
| EaeI | YGGCCR | 1 cut(s) | 3 |
| Eam1104I | CTCTTC | 4 cut(s) | 336, 651, 824, 921 |
| EarI | CTCTTC | 4 cut(s) | 336, 651, 824, 921 |
| Ecl136II | GAGCTC | 2 cut(s) | 794, 1399 |
| Eco130I | CCWWGG | 4 cut(s) | 1218, 1262, 1421, 1551 |
| Eco147I | AGGCCT | 1 cut(s) | 410 |
| Eco24I | GRGCYC | 3 cut(s) | 540, 796, 1401 |
| Eco31I | GGTCTC | 1 cut(s) | 569 |
| Eco32I | GATATC | 1 cut(s) | 1561 |
| Eco47I | GGWCC | 4 cut(s) | 20, 299, 1186, 1925 |
| Eco53kI | GAGCTC | 2 cut(s) | 794, 1399 |
| EcoICRI | GAGCTC | 2 cut(s) | 794, 1399 |
| EcoO109I | RGGNCCY | 2 cut(s) | 299, 1186 |
| EcoRII | CCWGG | 4 cut(s) | 105, 180, 1324, 1851 |
| EcoRV | GATATC | 1 cut(s) | 1561 |
| EcoT14I | CCWWGG | 4 cut(s) | 1218, 1262, 1421, 1551 |
| EcoT38I | GRGCYC | 3 cut(s) | 540, 796, 1401 |
| ErhI | CCWWGG | 4 cut(s) | 1218, 1262, 1421, 1551 |
| FaeI | CATG | 8 cut(s) | 193, 319, 940, 961, 1016, 1666, 1876, 1888 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 587, 619 |
| FaqI | GGGAC | 2 cut(s) | 33, 1911 |
| FatI | CATG | 8 cut(s) | 189, 315, 936, 957, 1012, 1662, 1872, 1884 |
| FauI | CCCGC | 2 cut(s) | 387, 1376 |
| FokI | GGATG | 7 cut(s) | 503, 739, 829, 1127, 1457, 1745, 1901 |
| FriOI | GRGCYC | 3 cut(s) | 540, 796, 1401 |
| FspBI | CTAG | 2 cut(s) | 1041, 1199 |
| GsuI | CTGGAG | 4 cut(s) | 516, 1257, 1398, 1664 |
| HaeIII | GGCC | 3 cut(s) | 5, 280, 410 |
| HapII | CCGG | 2 cut(s) | 6, 1112 |
| HgaI | GACGC | 3 cut(s) | 49, 176, 464 |
| Hin1I | GRCGYC | 1 cut(s) | 168 |
| Hin1II | CATG | 8 cut(s) | 193, 319, 940, 961, 1016, 1666, 1876, 1888 |
| HincII | GTYRAC | 1 cut(s) | 202 |
| HindII | GTYRAC | 1 cut(s) | 202 |
| HindIII | AAGCTT | 1 cut(s) | 1700 |
| HinfI | GANTC | 8 cut(s) | 467, 498, 733, 835, 872, 1045, 1336, 1628 |
| HpaII | CCGG | 2 cut(s) | 6, 1112 |
| Hpy166II | GTNNAC | 2 cut(s) | 202, 1430 |
| Hpy188I | TCNGA | 5 cut(s) | 65, 855, 1281, 1315, 1600 |
| Hpy8I | GTNNAC | 2 cut(s) | 202, 1430 |
| Hpy99I | CGWCG | 1 cut(s) | 65 |
| HpyAV | CCTTC | 9 cut(s) | 231, 292, 481, 568, 763, 1171, 1282, 1615, 1801 |
| HpyCH4III | ACNGT | 7 cut(s) | 208, 256, 427, 448, 1252, 1276, 1444 |
| HpyCH4IV | ACGT | 1 cut(s) | 1896 |
| HpyF10VI | GCNNNNNNNGC | 5 cut(s) | 302, 554, 1063, 1133, 1145 |
| HpyF3I | CTNAG | 8 cut(s) | 150, 174, 360, 797, 852, 1284, 1488, 1584 |
| HpySE526I | ACGT | 1 cut(s) | 1896 |
| Hsp92I | GRCGYC | 1 cut(s) | 168 |
| Hsp92II | CATG | 8 cut(s) | 193, 319, 940, 961, 1016, 1666, 1876, 1888 |
| Kzo9I | GATC | 8 cut(s) | 27, 79, 90, 159, 366, 613, 1257, 1833 |
| LguI | GCTCTTC | 1 cut(s) | 921 |
| LmnI | GCTCC | 7 cut(s) | 961, 1200, 1291, 1423, 1609, 1810, 1870 |
| MabI | ACCWGGT | 2 cut(s) | 180, 1324 |
| MaeI | CTAG | 2 cut(s) | 1041, 1199 |
| MaeII | ACGT | 1 cut(s) | 1896 |
| MaeIII | GTNAC | 4 cut(s) | 164, 245, 442, 1204 |
| MalI | GATC | 8 cut(s) | 29, 81, 92, 161, 368, 615, 1259, 1835 |
| MboI | GATC | 8 cut(s) | 27, 79, 90, 159, 366, 613, 1257, 1833 |
| MfeI | CAATTG | 3 cut(s) | 651, 1061, 1737 |
| MflI | RGATCY | 1 cut(s) | 366 |
| MhlI | GDGCHC | 4 cut(s) | 540, 796, 1401, 1614 |
| MluCI | AATT | 8 cut(s) | 350, 651, 1061, 1391, 1648, 1674, 1737, 1749 |
| MlyI | GAGTC | 5 cut(s) | 461, 507, 829, 866, 1637 |
| MmeI | TCCRAC | 4 cut(s) | 43, 568, 1304, 1578 |
| MroXI | GAANNNNTTC | 1 cut(s) | 1896 |
| MseI | TTAA | 3 cut(s) | 513, 1677, 1948 |
| MslI | CAYNNNNRTG | 3 cut(s) | 81, 320, 389 |
| MspI | CCGG | 2 cut(s) | 6, 1112 |
| MspR9I | CCNGG | 5 cut(s) | 107, 182, 1112, 1326, 1853 |
| MunI | CAATTG | 3 cut(s) | 651, 1061, 1737 |
| Mva1269I | GAATGC | 2 cut(s) | 1407, 1642 |
| MvaI | CCWGG | 4 cut(s) | 107, 182, 1326, 1853 |
| MvnI | CGCG | 1 cut(s) | 1371 |
| MwoI | GCNNNNNNNGC | 5 cut(s) | 302, 554, 1063, 1133, 1145 |
| NciI | CCSGG | 1 cut(s) | 1112 |
| NdeII | GATC | 8 cut(s) | 27, 79, 90, 159, 366, 613, 1257, 1833 |
| NlaIII | CATG | 8 cut(s) | 193, 319, 940, 961, 1016, 1666, 1876, 1888 |
| NlaIV | GGNNCC | 4 cut(s) | 21, 46, 963, 1927 |
| NmeAIII | GCCGAG | 2 cut(s) | 21, 396 |
| NmuCI | GTSAC | 3 cut(s) | 164, 245, 1204 |
| NspI | RCATGY | 1 cut(s) | 1666 |
| OliI | CACNNNNGTG | 1 cut(s) | 81 |
| PaqCI | CACCTGC | 1 cut(s) | 1285 |
| PceI | AGGCCT | 1 cut(s) | 410 |
| PciSI | GCTCTTC | 1 cut(s) | 921 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 84 |
| PctI | GAATGC | 2 cut(s) | 1407, 1642 |
| PdmI | GAANNNNTTC | 1 cut(s) | 1896 |
| PfeI | GAWTC | 3 cut(s) | 733, 1045, 1336 |
| Ple19I | CGATCG | 2 cut(s) | 30, 82 |
| PleI | GAGTC | 5 cut(s) | 461, 506, 829, 866, 1636 |
| PpsI | GAGTC | 5 cut(s) | 461, 506, 829, 866, 1636 |
| PpuMI | RGGWCCY | 2 cut(s) | 299, 1186 |
| Psp124BI | GAGCTC | 2 cut(s) | 796, 1401 |
| Psp5II | RGGWCCY | 2 cut(s) | 299, 1186 |
| Psp6I | CCWGG | 4 cut(s) | 105, 180, 1324, 1851 |
| PspGI | CCWGG | 4 cut(s) | 105, 180, 1324, 1851 |
| PspN4I | GGNNCC | 4 cut(s) | 21, 46, 963, 1927 |
| PspPI | GGNCC | 5 cut(s) | 20, 279, 299, 1186, 1925 |
| PspPPI | RGGWCCY | 2 cut(s) | 299, 1186 |
| PstI | CTGCAG | 1 cut(s) | 550 |
| PstNI | CAGNNNCTG | 1 cut(s) | 302 |
| PsuI | RGATCY | 1 cut(s) | 366 |
| PvuI | CGATCG | 2 cut(s) | 30, 82 |
| RsaI | GTAC | 3 cut(s) | 1431, 1575, 1850 |
| RsaNI | GTAC | 3 cut(s) | 1430, 1574, 1849 |
| RseI | CAYNNNNRTG | 3 cut(s) | 81, 320, 389 |
| SacI | GAGCTC | 2 cut(s) | 796, 1401 |
| SapI | GCTCTTC | 1 cut(s) | 921 |
| SaqAI | TTAA | 3 cut(s) | 513, 1677, 1948 |
| Sau3AI | GATC | 8 cut(s) | 27, 79, 90, 159, 366, 613, 1257, 1833 |
| Sau96I | GGNCC | 5 cut(s) | 20, 279, 299, 1186, 1925 |
| SchI | GAGTC | 5 cut(s) | 461, 507, 829, 866, 1637 |
| ScrFI | CCNGG | 5 cut(s) | 107, 182, 1112, 1326, 1853 |
| SduI | GDGCHC | 4 cut(s) | 540, 796, 1401, 1614 |
| SexAI | ACCWGGT | 2 cut(s) | 180, 1324 |
| SfcI | CTRYAG | 1 cut(s) | 546 |
| SinI | GGWCC | 4 cut(s) | 20, 299, 1186, 1925 |
| SmiMI | CAYNNNNRTG | 3 cut(s) | 81, 320, 389 |
| SmlI | CTYRAG | 1 cut(s) | 979 |
| SmoI | CTYRAG | 1 cut(s) | 979 |
| Sse9I | AATT | 8 cut(s) | 350, 651, 1061, 1391, 1648, 1674, 1737, 1749 |
| SseBI | AGGCCT | 1 cut(s) | 410 |
| SsiI | CCGC | 7 cut(s) | 171, 223, 394, 1139, 1369, 1666, 1881 |
| SspMI | CTAG | 2 cut(s) | 1041, 1199 |
| SstI | GAGCTC | 2 cut(s) | 796, 1401 |
| StuI | AGGCCT | 1 cut(s) | 410 |
| StyD4I | CCNGG | 5 cut(s) | 105, 180, 1110, 1324, 1851 |
| StyI | CCWWGG | 4 cut(s) | 1218, 1262, 1421, 1551 |
| TaaI | ACNGT | 7 cut(s) | 208, 256, 427, 448, 1252, 1276, 1444 |
| TaiI | ACGT | 1 cut(s) | 1899 |
| TaqI | TCGA | 6 cut(s) | 87, 213, 567, 1452, 1563, 1944 |
| TasI | AATT | 8 cut(s) | 350, 651, 1061, 1391, 1648, 1674, 1737, 1749 |
| TauI | GCSGC | 2 cut(s) | 173, 1884 |
| TfiI | GAWTC | 3 cut(s) | 733, 1045, 1336 |
| Tru1I | TTAA | 3 cut(s) | 513, 1677, 1948 |
| Tru9I | TTAA | 3 cut(s) | 513, 1677, 1948 |
| TscAI | CASTG | 8 cut(s) | 250, 281, 547, 589, 853, 1012, 1507, 1921 |
| TseFI | GTSAC | 3 cut(s) | 164, 245, 1204 |
| Tsp45I | GTSAC | 3 cut(s) | 164, 245, 1204 |
| TspDTI | ATGAA | 5 cut(s) | 206, 318, 891, 1131, 1905 |
| TspRI | CASTG | 8 cut(s) | 250, 281, 547, 589, 853, 1012, 1507, 1921 |
| VpaK11BI | GGWCC | 4 cut(s) | 20, 299, 1186, 1925 |
| XapI | RAATTY | 1 cut(s) | 1391 |
| XceI | RCATGY | 1 cut(s) | 1666 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 1639 |
| XmnI | GAANNNNTTC | 1 cut(s) | 1896 |
| XspI | CTAG | 2 cut(s) | 1041, 1199 |