Belongs to the heat shock protein 70 family
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 29 |
| AciI | CCGC | 2 cut(s) | 183, 279 |
| AclWI | GGATC | 1 cut(s) | 387 |
| AcoI | YGGCCR | 1 cut(s) | 177 |
| AcsI | RAATTY | 4 cut(s) | 419, 439, 543, 986 |
| AcuI | CTGAAG | 1 cut(s) | 1104 |
| AcyI | GRCGYC | 1 cut(s) | 162 |
| AfaI | GTAC | 2 cut(s) | 127, 893 |
| AflIII | ACRYGT | 1 cut(s) | 782 |
| AjuI | GAANNNNNNNTTGG | 4 cut(s) | 251, 283, 860, 892 |
| AloI | GAACNNNNNNTCC | 2 cut(s) | 624, 656 |
| AluBI | AGCT | 7 cut(s) | 77, 293, 562, 638, 647, 994, 1013 |
| AluI | AGCT | 7 cut(s) | 77, 293, 562, 638, 647, 994, 1013 |
| Alw21I | GWGCWC | 1 cut(s) | 295 |
| Alw26I | GTCTC | 3 cut(s) | 183, 328, 1081 |
| AlwI | GGATC | 1 cut(s) | 387 |
| Ama87I | CYCGRG | 2 cut(s) | 286, 411 |
| AoxI | GGCC | 3 cut(s) | 7, 61, 177 |
| ApeKI | GCWGC | 4 cut(s) | 44, 47, 635, 647 |
| ApoI | RAATTY | 4 cut(s) | 419, 439, 543, 986 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 443 |
| AsuHPI | GGTGA | 7 cut(s) | 167, 188, 218, 241, 754, 959, 995 |
| AvaI | CYCGRG | 2 cut(s) | 286, 411 |
| BalI | TGGCCA | 1 cut(s) | 179 |
| BanII | GRGCYC | 1 cut(s) | 295 |
| BbsI | GAAGAC | 1 cut(s) | 216 |
| Bbv12I | GWGCWC | 1 cut(s) | 295 |
| BbvI | GCAGC | 4 cut(s) | 31, 59, 634, 647 |
| BccI | CCATC | 3 cut(s) | 152, 169, 835 |
| BceAI | ACGGC | 2 cut(s) | 76, 1078 |
| BclI | TGATCA | 2 cut(s) | 805, 930 |
| BcoDI | GTCTC | 3 cut(s) | 183, 328, 1081 |
| BfaI | CTAG | 1 cut(s) | 540 |
| BfmI | CTRYAG | 3 cut(s) | 45, 129, 648 |
| BisI | GCNGC | 4 cut(s) | 45, 48, 636, 648 |
| BlsI | GCNGC | 4 cut(s) | 46, 49, 637, 649 |
| BmeT110I | CYCGRG | 2 cut(s) | 286, 411 |
| BmiI | GGNNCC | 1 cut(s) | 462 |
| BmsI | GCATC | 4 cut(s) | 475, 612, 1045, 1074 |
| BpiI | GAAGAC | 1 cut(s) | 216 |
| BpmI | CTGGAG | 2 cut(s) | 207, 993 |
| Bsa29I | ATCGAT | 1 cut(s) | 369 |
| BsaBI | GATNNNNATC | 1 cut(s) | 804 |
| BsaHI | GRCGYC | 1 cut(s) | 162 |
| BsaJI | CCNNGG | 1 cut(s) | 199 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 624, 654 |
| Bse1I | ACTGG | 2 cut(s) | 150, 1102 |
| Bse3DI | GCAATG | 1 cut(s) | 51 |
| Bse8I | GATNNNNATC | 1 cut(s) | 804 |
| BseCI | ATCGAT | 1 cut(s) | 369 |
| BseDI | CCNNGG | 1 cut(s) | 199 |
| BseGI | GGATG | 4 cut(s) | 517, 577, 763, 1065 |
| BseJI | GATNNNNATC | 1 cut(s) | 804 |
| BseMI | GCAATG | 1 cut(s) | 51 |
| BseNI | ACTGG | 2 cut(s) | 150, 1102 |
| BseRI | GAGGAG | 1 cut(s) | 346 |
| BseXI | GCAGC | 4 cut(s) | 31, 59, 634, 647 |
| BseYI | CCCAGC | 1 cut(s) | 500 |
| BshFI | GGCC | 3 cut(s) | 9, 63, 179 |
| BshVI | ATCGAT | 1 cut(s) | 369 |
| BsiHKAI | GWGCWC | 1 cut(s) | 295 |
| BsiHKCI | CYCGRG | 2 cut(s) | 286, 411 |
| BsmAI | GTCTC | 3 cut(s) | 183, 328, 1081 |
| BsnI | GGCC | 3 cut(s) | 9, 63, 179 |
| BsoBI | CYCGRG | 2 cut(s) | 286, 411 |
| Bsp1286I | GDGCHC | 1 cut(s) | 295 |
| Bsp143I | GATC | 4 cut(s) | 379, 805, 817, 930 |
| BspACI | CCGC | 2 cut(s) | 183, 279 |
| BspANI | GGCC | 3 cut(s) | 9, 63, 179 |
| BspDI | ATCGAT | 1 cut(s) | 369 |
| BspHI | TCATGA | 1 cut(s) | 814 |
| BspLI | GGNNCC | 1 cut(s) | 462 |
| BspMAI | CTGCAG | 2 cut(s) | 49, 652 |
| BspPI | GGATC | 1 cut(s) | 387 |
| BspQI | GCTCTTC | 1 cut(s) | 541 |
| BsrDI | GCAATG | 1 cut(s) | 51 |
| BsrI | ACTGG | 2 cut(s) | 150, 1102 |
| BssECI | CCNNGG | 1 cut(s) | 199 |
| BssMI | GATC | 4 cut(s) | 379, 805, 817, 930 |
| BssNI | GRCGYC | 1 cut(s) | 162 |
| BssT1I | CCWWGG | 1 cut(s) | 199 |
| Bst4CI | ACNGT | 3 cut(s) | 100, 239, 339 |
| Bst6I | CTCTTC | 1 cut(s) | 541 |
| BstACI | GRCGYC | 1 cut(s) | 162 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 53 |
| BstC8I | GCNNGC | 2 cut(s) | 7, 490 |
| BstDEI | CTNAG | 1 cut(s) | 1029 |
| BstF5I | GGATG | 4 cut(s) | 517, 577, 763, 1065 |
| BstKTI | GATC | 4 cut(s) | 382, 808, 820, 933 |
| BstMAI | GTCTC | 3 cut(s) | 183, 328, 1081 |
| BstMBI | GATC | 4 cut(s) | 379, 805, 817, 930 |
| BstMWI | GCNNNNNNNGC | 3 cut(s) | 53, 632, 644 |
| BstNSI | RCATGY | 2 cut(s) | 318, 786 |
| BstSFI | CTRYAG | 3 cut(s) | 45, 129, 648 |
| BstV1I | GCAGC | 4 cut(s) | 31, 59, 634, 647 |
| BstV2I | GAAGAC | 1 cut(s) | 216 |
| Bsu15I | ATCGAT | 1 cut(s) | 369 |
| BsuRI | GGCC | 3 cut(s) | 9, 63, 179 |
| BsuTUI | ATCGAT | 1 cut(s) | 369 |
| BtsCI | GGATG | 4 cut(s) | 517, 577, 763, 1065 |
| BtsIMutI | CAGTG | 2 cut(s) | 940, 1095 |
| Cac8I | GCNNGC | 2 cut(s) | 7, 490 |
| CciI | TCATGA | 1 cut(s) | 814 |
| ClaI | ATCGAT | 1 cut(s) | 369 |
| CseI | GACGC | 2 cut(s) | 151, 506 |
| Csp6I | GTAC | 2 cut(s) | 126, 892 |
| CviAII | CATG | 5 cut(s) | 315, 436, 493, 783, 815 |
| CviQI | GTAC | 2 cut(s) | 126, 892 |
| DdeI | CTNAG | 1 cut(s) | 1029 |
| DpnI | GATC | 4 cut(s) | 381, 807, 819, 932 |
| DpnII | GATC | 4 cut(s) | 379, 805, 817, 930 |
| EaeI | YGGCCR | 1 cut(s) | 177 |
| Eam1104I | CTCTTC | 1 cut(s) | 541 |
| EarI | CTCTTC | 1 cut(s) | 541 |
| Ecl136II | GAGCTC | 1 cut(s) | 293 |
| Eco130I | CCWWGG | 1 cut(s) | 199 |
| Eco24I | GRGCYC | 1 cut(s) | 295 |
| Eco53kI | GAGCTC | 1 cut(s) | 293 |
| Eco57I | CTGAAG | 1 cut(s) | 1104 |
| Eco88I | CYCGRG | 2 cut(s) | 286, 411 |
| EcoICRI | GAGCTC | 1 cut(s) | 293 |
| EcoRI | GAATTC | 1 cut(s) | 543 |
| EcoT14I | CCWWGG | 1 cut(s) | 199 |
| EcoT22I | ATGCAT | 1 cut(s) | 316 |
| EcoT38I | GRGCYC | 1 cut(s) | 295 |
| ErhI | CCWWGG | 1 cut(s) | 199 |
| FaeI | CATG | 5 cut(s) | 318, 439, 496, 786, 818 |
| FatI | CATG | 5 cut(s) | 314, 435, 492, 782, 814 |
| FauI | CCCGC | 1 cut(s) | 272 |
| FauNDI | CATATG | 1 cut(s) | 628 |
| FbaI | TGATCA | 2 cut(s) | 805, 930 |
| Fnu4HI | GCNGC | 4 cut(s) | 45, 48, 636, 648 |
| FokI | GGATG | 4 cut(s) | 524, 584, 770, 1052 |
| FriOI | GRGCYC | 1 cut(s) | 295 |
| Fsp4HI | GCNGC | 4 cut(s) | 45, 48, 636, 648 |
| FspBI | CTAG | 1 cut(s) | 540 |
| GluI | GCNGC | 4 cut(s) | 45, 48, 636, 648 |
| GsaI | CCCAGC | 1 cut(s) | 504 |
| GsuI | CTGGAG | 2 cut(s) | 207, 993 |
| HaeIII | GGCC | 3 cut(s) | 9, 63, 179 |
| HgaI | GACGC | 2 cut(s) | 151, 506 |
| Hin1I | GRCGYC | 1 cut(s) | 162 |
| Hin1II | CATG | 5 cut(s) | 318, 439, 496, 786, 818 |
| HincII | GTYRAC | 1 cut(s) | 358 |
| HindII | GTYRAC | 1 cut(s) | 358 |
| HinfI | GANTC | 2 cut(s) | 19, 853 |
| HphI | GGTGA | 7 cut(s) | 167, 188, 218, 241, 754, 959, 995 |
| Hpy166II | GTNNAC | 3 cut(s) | 232, 358, 1095 |
| Hpy188I | TCNGA | 5 cut(s) | 85, 549, 757, 829, 910 |
| Hpy188III | TCNNGA | 7 cut(s) | 253, 288, 540, 611, 815, 880, 1143 |
| Hpy8I | GTNNAC | 3 cut(s) | 232, 358, 1095 |
| Hpy99I | CGWCG | 2 cut(s) | 825, 1064 |
| HpyAV | CCTTC | 5 cut(s) | 316, 544, 580, 886, 938 |
| HpyCH4III | ACNGT | 3 cut(s) | 100, 239, 339 |
| HpyCH4IV | ACGT | 1 cut(s) | 273 |
| HpyCH4V | TGCA | 9 cut(s) | 47, 314, 401, 492, 556, 568, 626, 650, 698 |
| HpyF10VI | GCNNNNNNNGC | 3 cut(s) | 53, 632, 644 |
| HpyF3I | CTNAG | 1 cut(s) | 1029 |
| HpySE526I | ACGT | 1 cut(s) | 273 |
| Hsp92I | GRCGYC | 1 cut(s) | 162 |
| Hsp92II | CATG | 5 cut(s) | 318, 439, 496, 786, 818 |
| Ksp22I | TGATCA | 2 cut(s) | 805, 930 |
| Kzo9I | GATC | 4 cut(s) | 379, 805, 817, 930 |
| LguI | GCTCTTC | 1 cut(s) | 541 |
| LmnI | GCTCC | 3 cut(s) | 460, 632, 1018 |
| Lsp1109I | GCAGC | 4 cut(s) | 31, 59, 634, 647 |
| LweI | GCATC | 4 cut(s) | 475, 612, 1045, 1074 |
| MaeI | CTAG | 1 cut(s) | 540 |
| MaeII | ACGT | 1 cut(s) | 273 |
| MaeIII | GTNAC | 1 cut(s) | 760 |
| MalI | GATC | 4 cut(s) | 381, 807, 819, 932 |
| MboI | GATC | 4 cut(s) | 379, 805, 817, 930 |
| MboII | GAAGA | 3 cut(s) | 221, 436, 528 |
| MfeI | CAATTG | 1 cut(s) | 428 |
| MhlI | GDGCHC | 1 cut(s) | 295 |
| MlsI | TGGCCA | 1 cut(s) | 179 |
| MluCI | AATT | 7 cut(s) | 248, 419, 428, 439, 543, 590, 986 |
| MluNI | TGGCCA | 1 cut(s) | 179 |
| MnlI | CCTC | 7 cut(s) | 74, 197, 249, 294, 324, 610, 1017 |
| Mox20I | TGGCCA | 1 cut(s) | 179 |
| Mph1103I | ATGCAT | 1 cut(s) | 316 |
| MroXI | GAANNNNTTC | 1 cut(s) | 443 |
| MscI | TGGCCA | 1 cut(s) | 179 |
| MseI | TTAA | 6 cut(s) | 656, 704, 738, 977, 990, 1071 |
| MslI | CAYNNNNRTG | 1 cut(s) | 813 |
| Msp20I | TGGCCA | 1 cut(s) | 179 |
| MspA1I | CMGCKG | 2 cut(s) | 185, 638 |
| MunI | CAATTG | 1 cut(s) | 428 |
| MwoI | GCNNNNNNNGC | 3 cut(s) | 53, 632, 644 |
| NdeI | CATATG | 1 cut(s) | 628 |
| NdeII | GATC | 4 cut(s) | 379, 805, 817, 930 |
| NlaIII | CATG | 5 cut(s) | 318, 439, 496, 786, 818 |
| NlaIV | GGNNCC | 1 cut(s) | 462 |
| NmeAIII | GCCGAG | 1 cut(s) | 268 |
| NmuCI | GTSAC | 1 cut(s) | 760 |
| NsiI | ATGCAT | 1 cut(s) | 316 |
| NspI | RCATGY | 2 cut(s) | 318, 786 |
| PaeR7I | CTCGAG | 1 cut(s) | 286 |
| PagI | TCATGA | 1 cut(s) | 814 |
| PciI | ACATGT | 1 cut(s) | 782 |
| PciSI | GCTCTTC | 1 cut(s) | 541 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 270 |
| PdmI | GAANNNNTTC | 1 cut(s) | 443 |
| PfeI | GAWTC | 2 cut(s) | 19, 853 |
| PkrI | GCNGC | 4 cut(s) | 46, 49, 637, 649 |
| PscI | ACATGT | 1 cut(s) | 782 |
| PsiI | TTATAA | 1 cut(s) | 29 |
| Psp124BI | GAGCTC | 1 cut(s) | 295 |
| PspFI | CCCAGC | 1 cut(s) | 500 |
| PspN4I | GGNNCC | 1 cut(s) | 462 |
| PstI | CTGCAG | 2 cut(s) | 49, 652 |
| PvuII | CAGCTG | 1 cut(s) | 638 |
| RsaI | GTAC | 2 cut(s) | 127, 893 |
| RsaNI | GTAC | 2 cut(s) | 126, 892 |
| RseI | CAYNNNNRTG | 1 cut(s) | 813 |
| SacI | GAGCTC | 1 cut(s) | 295 |
| SapI | GCTCTTC | 1 cut(s) | 541 |
| SaqAI | TTAA | 6 cut(s) | 656, 704, 738, 977, 990, 1071 |
| SatI | GCNGC | 4 cut(s) | 45, 48, 636, 648 |
| Sau3AI | GATC | 4 cut(s) | 379, 805, 817, 930 |
| SduI | GDGCHC | 1 cut(s) | 295 |
| SfaNI | GCATC | 4 cut(s) | 475, 612, 1045, 1074 |
| SfcI | CTRYAG | 3 cut(s) | 45, 129, 648 |
| Sfr274I | CTCGAG | 1 cut(s) | 286 |
| SlaI | CTCGAG | 1 cut(s) | 286 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 813 |
| SmlI | CTYRAG | 1 cut(s) | 286 |
| SmoI | CTYRAG | 1 cut(s) | 286 |
| Sse9I | AATT | 7 cut(s) | 248, 419, 428, 439, 543, 590, 986 |
| SsiI | CCGC | 2 cut(s) | 183, 279 |
| SspMI | CTAG | 1 cut(s) | 540 |
| SstI | GAGCTC | 1 cut(s) | 295 |
| StyI | CCWWGG | 1 cut(s) | 199 |
| TaaI | ACNGT | 3 cut(s) | 100, 239, 339 |
| TaiI | ACGT | 1 cut(s) | 276 |
| TaqI | TCGA | 4 cut(s) | 66, 287, 363, 369 |
| TasI | AATT | 7 cut(s) | 248, 419, 428, 439, 543, 590, 986 |
| TfiI | GAWTC | 2 cut(s) | 19, 853 |
| Tru1I | TTAA | 6 cut(s) | 656, 704, 738, 977, 990, 1071 |
| Tru9I | TTAA | 6 cut(s) | 656, 704, 738, 977, 990, 1071 |
| TscAI | CASTG | 2 cut(s) | 940, 1102 |
| TseFI | GTSAC | 1 cut(s) | 760 |
| TseI | GCWGC | 4 cut(s) | 44, 47, 635, 647 |
| Tsp45I | GTSAC | 1 cut(s) | 760 |
| TspDTI | ATGAA | 4 cut(s) | 48, 452, 803, 899 |
| TspRI | CASTG | 2 cut(s) | 940, 1102 |
| XapI | RAATTY | 4 cut(s) | 419, 439, 543, 986 |
| XbaI | TCTAGA | 1 cut(s) | 539 |
| XceI | RCATGY | 2 cut(s) | 318, 786 |
| XhoI | CTCGAG | 1 cut(s) | 286 |
| XmnI | GAANNNNTTC | 1 cut(s) | 443 |
| XspI | CTAG | 1 cut(s) | 540 |
| Zsp2I | ATGCAT | 1 cut(s) | 316 |