Belongs to the heat shock protein 70 family
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccB1I | GGYRCC | 1 cut(s) | 1270 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 1154 |
| AccBSI | CCGCTC | 2 cut(s) | 14, 28 |
| AccII | CGCG | 1 cut(s) | 687 |
| AciI | CCGC | 3 cut(s) | 14, 28, 687 |
| AclWI | GGATC | 6 cut(s) | 42, 152, 656, 669, 794, 1365 |
| AcoI | YGGCCR | 1 cut(s) | 1045 |
| AcsI | RAATTY | 2 cut(s) | 393, 751 |
| AcuI | CTGAAG | 6 cut(s) | 27, 162, 339, 911, 1028, 1295 |
| AfaI | GTAC | 3 cut(s) | 811, 981, 1326 |
| AfiI | CCNNNNNNNGG | 7 cut(s) | 167, 311, 947, 1148, 1154, 1352, 1391 |
| AgsI | TTSAA | 6 cut(s) | 463, 494, 540, 620, 735, 756 |
| AjnI | CCWGG | 6 cut(s) | 631, 791, 940, 1147, 1318, 1384 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 312, 344 |
| AluBI | AGCT | 4 cut(s) | 104, 373, 652, 766 |
| AluI | AGCT | 4 cut(s) | 104, 373, 652, 766 |
| Alw21I | GWGCWC | 2 cut(s) | 645, 1006 |
| AlwI | GGATC | 6 cut(s) | 42, 152, 656, 669, 794, 1365 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 1193 |
| AoxI | GGCC | 6 cut(s) | 334, 1045, 1121, 1244, 1343, 1436 |
| ApeKI | GCWGC | 5 cut(s) | 827, 830, 1222, 1315, 1404 |
| ApoI | RAATTY | 2 cut(s) | 393, 751 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 1354, 1386 |
| AseI | ATTAAT | 1 cut(s) | 410 |
| AspLEI | GCGC | 2 cut(s) | 909, 1193 |
| AspS9I | GGNCC | 5 cut(s) | 591, 629, 1121, 1244, 1370 |
| AsuC2I | CCSGG | 1 cut(s) | 169 |
| AsuHPI | GGTGA | 4 cut(s) | 233, 443, 850, 926 |
| AsuII | TTCGAA | 1 cut(s) | 607 |
| AsuNHI | GCTAGC | 1 cut(s) | 173 |
| AvaII | GGWCC | 3 cut(s) | 591, 629, 1370 |
| BalI | TGGCCA | 1 cut(s) | 1047 |
| BamHI | GGATCC | 1 cut(s) | 661 |
| BanI | GGYRCC | 1 cut(s) | 1270 |
| Bbv12I | GWGCWC | 2 cut(s) | 645, 1006 |
| BbvI | GCAGC | 5 cut(s) | 814, 817, 1234, 1327, 1416 |
| BccI | CCATC | 9 cut(s) | 58, 92, 101, 1037, 1056, 1091, 1261, 1261, 1283 |
| BceAI | ACGGC | 2 cut(s) | 501, 1195 |
| BciT130I | CCWGG | 6 cut(s) | 633, 793, 942, 1149, 1320, 1386 |
| BclI | TGATCA | 1 cut(s) | 817 |
| BcnI | CCSGG | 1 cut(s) | 169 |
| BfaI | CTAG | 4 cut(s) | 38, 174, 876, 1055 |
| BfmI | CTRYAG | 1 cut(s) | 1185 |
| BfoI | RGCGCY | 2 cut(s) | 910, 1194 |
| BisI | GCNGC | 5 cut(s) | 828, 831, 1223, 1316, 1405 |
| BlsI | GCNGC | 5 cut(s) | 829, 832, 1224, 1317, 1406 |
| Bme1390I | CCNGG | 7 cut(s) | 169, 633, 793, 942, 1149, 1320, 1386 |
| Bme18I | GGWCC | 3 cut(s) | 591, 629, 1370 |
| BmgT120I | GGNCC | 5 cut(s) | 591, 629, 1121, 1244, 1370 |
| BmiI | GGNNCC | 2 cut(s) | 663, 1272 |
| BmrFI | CCNGG | 7 cut(s) | 169, 633, 793, 942, 1149, 1320, 1386 |
| BmsI | GCATC | 2 cut(s) | 337, 1320 |
| BmtI | GCTAGC | 1 cut(s) | 177 |
| BpmI | CTGGAG | 3 cut(s) | 177, 654, 788 |
| Bpu10I | CCTNAGC | 1 cut(s) | 834 |
| Bpu14I | TTCGAA | 1 cut(s) | 607 |
| BpuEI | CTTGAG | 1 cut(s) | 419 |
| BpuMI | CCSGG | 1 cut(s) | 169 |
| BsaBI | GATNNNNATC | 1 cut(s) | 1047 |
| BsaJI | CCNNGG | 9 cut(s) | 168, 594, 792, 910, 1147, 1148, 1319, 1340, 1385 |
| BsaXI | ACNNNNNCTCC | 3 cut(s) | 33, 1381, 1411 |
| Bsc4I | CCNNNNNNNGG | 7 cut(s) | 167, 311, 947, 1148, 1154, 1352, 1391 |
| Bse118I | RCCGGY | 1 cut(s) | 1434 |
| Bse1I | ACTGG | 1 cut(s) | 433 |
| Bse8I | GATNNNNATC | 1 cut(s) | 1047 |
| BseBI | CCWGG | 6 cut(s) | 633, 793, 942, 1149, 1320, 1386 |
| BseDI | CCNNGG | 9 cut(s) | 168, 594, 792, 910, 1147, 1148, 1319, 1340, 1385 |
| BseGI | GGATG | 3 cut(s) | 73, 604, 1048 |
| BseJI | GATNNNNATC | 1 cut(s) | 1047 |
| BseLI | CCNNNNNNNGG | 7 cut(s) | 167, 311, 947, 1148, 1154, 1352, 1391 |
| BseMII | CTCAG | 5 cut(s) | 274, 366, 848, 858, 1091 |
| BseNI | ACTGG | 1 cut(s) | 433 |
| BseRI | GAGGAG | 3 cut(s) | 284, 1329, 1407 |
| BseXI | GCAGC | 5 cut(s) | 814, 817, 1234, 1327, 1416 |
| BseYI | CCCAGC | 2 cut(s) | 1118, 1210 |
| Bsh1236I | CGCG | 1 cut(s) | 687 |
| BshFI | GGCC | 6 cut(s) | 336, 1047, 1123, 1246, 1345, 1438 |
| BshNI | GGYRCC | 1 cut(s) | 1270 |
| BsiHKAI | GWGCWC | 2 cut(s) | 645, 1006 |
| BsiSI | CCGG | 2 cut(s) | 168, 1435 |
| BslI | CCNNNNNNNGG | 7 cut(s) | 167, 311, 947, 1148, 1154, 1352, 1391 |
| BsmI | GAATGC | 3 cut(s) | 187, 449, 1266 |
| BsnI | GGCC | 6 cut(s) | 336, 1047, 1123, 1246, 1345, 1438 |
| Bsp119I | TTCGAA | 1 cut(s) | 607 |
| Bsp1286I | GDGCHC | 2 cut(s) | 645, 1006 |
| Bsp1407I | TGTACA | 1 cut(s) | 979 |
| Bsp143I | GATC | 6 cut(s) | 34, 157, 661, 799, 817, 1357 |
| Bsp19I | CCATGG | 1 cut(s) | 910 |
| BspACI | CCGC | 3 cut(s) | 14, 28, 687 |
| BspANI | GGCC | 6 cut(s) | 336, 1047, 1123, 1246, 1345, 1438 |
| BspCNI | CTCAG | 5 cut(s) | 273, 365, 847, 859, 1090 |
| BspFNI | CGCG | 1 cut(s) | 687 |
| BspLI | GGNNCC | 2 cut(s) | 663, 1272 |
| BspMAI | CTGCAG | 1 cut(s) | 1189 |
| BspOI | GCTAGC | 1 cut(s) | 177 |
| BspPI | GGATC | 6 cut(s) | 42, 152, 656, 669, 794, 1365 |
| BspT104I | TTCGAA | 1 cut(s) | 607 |
| BspT107I | GGYRCC | 1 cut(s) | 1270 |
| BsrBI | CCGCTC | 2 cut(s) | 14, 28 |
| BsrFI | RCCGGY | 1 cut(s) | 1434 |
| BsrGI | TGTACA | 1 cut(s) | 979 |
| BsrI | ACTGG | 1 cut(s) | 433 |
| BssAI | RCCGGY | 1 cut(s) | 1434 |
| BssECI | CCNNGG | 9 cut(s) | 168, 594, 792, 910, 1147, 1148, 1319, 1340, 1385 |
| BssMI | GATC | 6 cut(s) | 34, 157, 661, 799, 817, 1357 |
| BssT1I | CCWWGG | 1 cut(s) | 910 |
| Bst2UI | CCWGG | 6 cut(s) | 633, 793, 942, 1149, 1320, 1386 |
| Bst4CI | ACNGT | 3 cut(s) | 218, 564, 787 |
| Bst6I | CTCTTC | 5 cut(s) | 54, 234, 354, 615, 1345 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 1270 |
| BstAUI | TGTACA | 1 cut(s) | 979 |
| BstBI | TTCGAA | 1 cut(s) | 607 |
| BstC8I | GCNNGC | 4 cut(s) | 175, 285, 297, 1189 |
| BstDEI | CTNAG | 5 cut(s) | 260, 352, 834, 867, 1077 |
| BstDSI | CCRYGG | 1 cut(s) | 910 |
| BstENI | CCTNNNNNAGG | 2 cut(s) | 945, 1350 |
| BstF5I | GGATG | 3 cut(s) | 73, 604, 1048 |
| BstFNI | CGCG | 1 cut(s) | 687 |
| BstH2I | RGCGCY | 2 cut(s) | 910, 1194 |
| BstHHI | GCGC | 2 cut(s) | 909, 1193 |
| BstKTI | GATC | 6 cut(s) | 37, 160, 664, 802, 820, 1360 |
| BstMBI | GATC | 6 cut(s) | 34, 157, 661, 799, 817, 1357 |
| BstMWI | GCNNNNNNNGC | 9 cut(s) | 11, 146, 485, 649, 1010, 1188, 1270, 1413, 1422 |
| BstNI | CCWGG | 6 cut(s) | 633, 793, 942, 1149, 1320, 1386 |
| BstNSI | RCATGY | 2 cut(s) | 140, 287 |
| BstSCI | CCNGG | 7 cut(s) | 167, 631, 791, 940, 1147, 1318, 1384 |
| BstSFI | CTRYAG | 1 cut(s) | 1185 |
| BstUI | CGCG | 1 cut(s) | 687 |
| BstV1I | GCAGC | 5 cut(s) | 814, 817, 1234, 1327, 1416 |
| BstX2I | RGATCY | 3 cut(s) | 34, 157, 661 |
| BstXI | CCANNNNNNTGG | 2 cut(s) | 588, 911 |
| BstYI | RGATCY | 3 cut(s) | 34, 157, 661 |
| BsuRI | GGCC | 6 cut(s) | 336, 1047, 1123, 1246, 1345, 1438 |
| BtgI | CCRYGG | 1 cut(s) | 910 |
| BtgZI | GCGATG | 1 cut(s) | 1422 |
| BtsCI | GGATG | 3 cut(s) | 73, 604, 1048 |
| BtsI | GCAGTG | 2 cut(s) | 121, 385 |
| BtsIMutI | CAGTG | 3 cut(s) | 121, 385, 879 |
| Cac8I | GCNNGC | 4 cut(s) | 175, 285, 297, 1189 |
| CaiI | CAGNNNCTG | 1 cut(s) | 1193 |
| CfoI | GCGC | 2 cut(s) | 909, 1193 |
| Cfr10I | RCCGGY | 1 cut(s) | 1434 |
| Cfr13I | GGNCC | 5 cut(s) | 591, 629, 1121, 1244, 1370 |
| Csp6I | GTAC | 3 cut(s) | 810, 980, 1325 |
| CviAII | CATG | 5 cut(s) | 55, 137, 284, 911, 1136 |
| CviQI | GTAC | 3 cut(s) | 810, 980, 1325 |
| DdeI | CTNAG | 5 cut(s) | 260, 352, 834, 867, 1077 |
| DpnI | GATC | 6 cut(s) | 36, 159, 663, 801, 819, 1359 |
| DpnII | GATC | 6 cut(s) | 34, 157, 661, 799, 817, 1357 |
| EaeI | YGGCCR | 1 cut(s) | 1045 |
| Eam1104I | CTCTTC | 5 cut(s) | 54, 234, 354, 615, 1345 |
| EarI | CTCTTC | 5 cut(s) | 54, 234, 354, 615, 1345 |
| Eco130I | CCWWGG | 1 cut(s) | 910 |
| Eco47I | GGWCC | 3 cut(s) | 591, 629, 1370 |
| Eco57I | CTGAAG | 6 cut(s) | 27, 162, 339, 911, 1028, 1295 |
| EcoNI | CCTNNNNNAGG | 2 cut(s) | 945, 1350 |
| EcoRII | CCWGG | 6 cut(s) | 631, 791, 940, 1147, 1318, 1384 |
| EcoT14I | CCWWGG | 1 cut(s) | 910 |
| EcoT22I | ATGCAT | 1 cut(s) | 451 |
| ErhI | CCWWGG | 1 cut(s) | 910 |
| FaeI | CATG | 5 cut(s) | 58, 140, 287, 914, 1139 |
| FatI | CATG | 5 cut(s) | 54, 136, 283, 910, 1135 |
| FauI | CCCGC | 1 cut(s) | 21 |
| FauNDI | CATATG | 1 cut(s) | 202 |
| FbaI | TGATCA | 1 cut(s) | 817 |
| Fnu4HI | GCNGC | 5 cut(s) | 828, 831, 1223, 1316, 1405 |
| FokI | GGATG | 3 cut(s) | 80, 611, 1035 |
| Fsp4HI | GCNGC | 5 cut(s) | 828, 831, 1223, 1316, 1405 |
| FspBI | CTAG | 4 cut(s) | 38, 174, 876, 1055 |
| GlaI | GCGC | 2 cut(s) | 908, 1192 |
| GluI | GCNGC | 5 cut(s) | 828, 831, 1223, 1316, 1405 |
| GsaI | CCCAGC | 2 cut(s) | 1122, 1214 |
| GsuI | CTGGAG | 3 cut(s) | 177, 654, 788 |
| HaeII | RGCGCY | 2 cut(s) | 910, 1194 |
| HaeIII | GGCC | 6 cut(s) | 336, 1047, 1123, 1246, 1345, 1438 |
| HapII | CCGG | 2 cut(s) | 168, 1435 |
| HhaI | GCGC | 2 cut(s) | 909, 1193 |
| Hin1II | CATG | 5 cut(s) | 58, 140, 287, 914, 1139 |
| Hin6I | GCGC | 2 cut(s) | 907, 1191 |
| HinP1I | GCGC | 2 cut(s) | 907, 1191 |
| HinfI | GANTC | 1 cut(s) | 459 |
| HpaII | CCGG | 2 cut(s) | 168, 1435 |
| HphI | GGTGA | 4 cut(s) | 233, 443, 850, 926 |
| Hpy166II | GTNNAC | 2 cut(s) | 982, 1379 |
| Hpy188I | TCNGA | 8 cut(s) | 319, 355, 547, 817, 1008, 1080, 1096, 1374 |
| Hpy188III | TCNNGA | 8 cut(s) | 38, 194, 266, 398, 589, 735, 866, 1257 |
| Hpy8I | GTNNAC | 2 cut(s) | 982, 1379 |
| HpyAV | CCTTC | 7 cut(s) | 258, 311, 347, 835, 886, 1003, 1406 |
| HpyCH4III | ACNGT | 3 cut(s) | 218, 564, 787 |
| HpyCH4V | TGCA | 6 cut(s) | 378, 415, 449, 479, 1187, 1264 |
| HpyF10VI | GCNNNNNNNGC | 9 cut(s) | 11, 146, 485, 649, 1010, 1188, 1270, 1413, 1422 |
| HpyF3I | CTNAG | 5 cut(s) | 260, 352, 834, 867, 1077 |
| Hsp92II | CATG | 5 cut(s) | 58, 140, 287, 914, 1139 |
| HspAI | GCGC | 2 cut(s) | 907, 1191 |
| Ksp22I | TGATCA | 1 cut(s) | 817 |
| Kzo9I | GATC | 6 cut(s) | 34, 157, 661, 799, 817, 1357 |
| LmnI | GCTCC | 4 cut(s) | 11, 101, 370, 1144 |
| Lsp1109I | GCAGC | 5 cut(s) | 814, 817, 1234, 1327, 1416 |
| LweI | GCATC | 2 cut(s) | 337, 1320 |
| MaeI | CTAG | 4 cut(s) | 38, 174, 876, 1055 |
| MaeIII | GTNAC | 2 cut(s) | 787, 1204 |
| MalI | GATC | 6 cut(s) | 36, 159, 663, 801, 819, 1359 |
| MbiI | CCGCTC | 2 cut(s) | 14, 28 |
| MboI | GATC | 6 cut(s) | 34, 157, 661, 799, 817, 1357 |
| MflI | RGATCY | 3 cut(s) | 34, 157, 661 |
| MhlI | GDGCHC | 2 cut(s) | 645, 1006 |
| MlsI | TGGCCA | 1 cut(s) | 1047 |
| MluCI | AATT | 6 cut(s) | 49, 393, 497, 540, 751, 1038 |
| MluNI | TGGCCA | 1 cut(s) | 1047 |
| Mox20I | TGGCCA | 1 cut(s) | 1047 |
| Mph1103I | ATGCAT | 1 cut(s) | 451 |
| MscI | TGGCCA | 1 cut(s) | 1047 |
| MseI | TTAA | 5 cut(s) | 410, 516, 530, 762, 1017 |
| Msp20I | TGGCCA | 1 cut(s) | 1047 |
| MspI | CCGG | 2 cut(s) | 168, 1435 |
| MspR9I | CCNGG | 7 cut(s) | 169, 633, 793, 942, 1149, 1320, 1386 |
| Mva1269I | GAATGC | 3 cut(s) | 187, 449, 1266 |
| MvaI | CCWGG | 6 cut(s) | 633, 793, 942, 1149, 1320, 1386 |
| MvnI | CGCG | 1 cut(s) | 687 |
| MwoI | GCNNNNNNNGC | 9 cut(s) | 11, 146, 485, 649, 1010, 1188, 1270, 1413, 1422 |
| NciI | CCSGG | 1 cut(s) | 169 |
| NcoI | CCATGG | 1 cut(s) | 910 |
| NdeI | CATATG | 1 cut(s) | 202 |
| NdeII | GATC | 6 cut(s) | 34, 157, 661, 799, 817, 1357 |
| NheI | GCTAGC | 1 cut(s) | 173 |
| NlaIII | CATG | 5 cut(s) | 58, 140, 287, 914, 1139 |
| NlaIV | GGNNCC | 2 cut(s) | 663, 1272 |
| NmeAIII | GCCGAG | 1 cut(s) | 1321 |
| NmuCI | GTSAC | 1 cut(s) | 787 |
| NsiI | ATGCAT | 1 cut(s) | 451 |
| NspI | RCATGY | 2 cut(s) | 140, 287 |
| NspV | TTCGAA | 1 cut(s) | 607 |
| PaeI | GCATGC | 1 cut(s) | 287 |
| PasI | CCCWGGG | 1 cut(s) | 1148 |
| PctI | GAATGC | 3 cut(s) | 187, 449, 1266 |
| PfeI | GAWTC | 1 cut(s) | 459 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 1154 |
| PfoI | TCCNGGA | 1 cut(s) | 631 |
| PkrI | GCNGC | 5 cut(s) | 829, 832, 1224, 1317, 1406 |
| PshBI | ATTAAT | 1 cut(s) | 410 |
| Psp6I | CCWGG | 6 cut(s) | 631, 791, 940, 1147, 1318, 1384 |
| PspFI | CCCAGC | 2 cut(s) | 1118, 1210 |
| PspGI | CCWGG | 6 cut(s) | 631, 791, 940, 1147, 1318, 1384 |
| PspN4I | GGNNCC | 2 cut(s) | 663, 1272 |
| PspPI | GGNCC | 5 cut(s) | 591, 629, 1121, 1244, 1370 |
| PstI | CTGCAG | 1 cut(s) | 1189 |
| PstNI | CAGNNNCTG | 1 cut(s) | 1193 |
| PsuI | RGATCY | 3 cut(s) | 34, 157, 661 |
| RsaI | GTAC | 3 cut(s) | 811, 981, 1326 |
| RsaNI | GTAC | 3 cut(s) | 810, 980, 1325 |
| SaqAI | TTAA | 5 cut(s) | 410, 516, 530, 762, 1017 |
| SatI | GCNGC | 5 cut(s) | 828, 831, 1223, 1316, 1405 |
| Sau3AI | GATC | 6 cut(s) | 34, 157, 661, 799, 817, 1357 |
| Sau96I | GGNCC | 5 cut(s) | 591, 629, 1121, 1244, 1370 |
| ScrFI | CCNGG | 7 cut(s) | 169, 633, 793, 942, 1149, 1320, 1386 |
| SduI | GDGCHC | 2 cut(s) | 645, 1006 |
| SfaNI | GCATC | 2 cut(s) | 337, 1320 |
| SfcI | CTRYAG | 1 cut(s) | 1185 |
| SfuI | TTCGAA | 1 cut(s) | 607 |
| SinI | GGWCC | 3 cut(s) | 591, 629, 1370 |
| SmlI | CTYRAG | 1 cut(s) | 398 |
| SmoI | CTYRAG | 1 cut(s) | 398 |
| SphI | GCATGC | 1 cut(s) | 287 |
| Sse9I | AATT | 6 cut(s) | 49, 393, 497, 540, 751, 1038 |
| SsiI | CCGC | 3 cut(s) | 14, 28, 687 |
| SspI | AATATT | 1 cut(s) | 900 |
| SspMI | CTAG | 4 cut(s) | 38, 174, 876, 1055 |
| StyD4I | CCNGG | 7 cut(s) | 167, 631, 791, 940, 1147, 1318, 1384 |
| StyI | CCWWGG | 1 cut(s) | 910 |
| TaaI | ACNGT | 3 cut(s) | 218, 564, 787 |
| TaqI | TCGA | 4 cut(s) | 607, 1294, 1348, 1360 |
| TaqII | GACCGA | 1 cut(s) | 608 |
| TasI | AATT | 6 cut(s) | 49, 393, 497, 540, 751, 1038 |
| TatI | WGTACW | 2 cut(s) | 809, 979 |
| TfiI | GAWTC | 1 cut(s) | 459 |
| Tru1I | TTAA | 5 cut(s) | 410, 516, 530, 762, 1017 |
| Tru9I | TTAA | 5 cut(s) | 410, 516, 530, 762, 1017 |
| TscAI | CASTG | 3 cut(s) | 121, 385, 886 |
| TseFI | GTSAC | 1 cut(s) | 787 |
| TseI | GCWGC | 5 cut(s) | 827, 830, 1222, 1315, 1404 |
| Tsp45I | GTSAC | 1 cut(s) | 787 |
| TspDTI | ATGAA | 8 cut(s) | 108, 135, 252, 316, 442, 556, 1050, 1425 |
| TspRI | CASTG | 3 cut(s) | 121, 385, 886 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 1154 |
| VpaK11BI | GGWCC | 3 cut(s) | 591, 629, 1370 |
| VspI | ATTAAT | 1 cut(s) | 410 |
| XagI | CCTNNNNNAGG | 2 cut(s) | 945, 1350 |
| XapI | RAATTY | 2 cut(s) | 393, 751 |
| XbaI | TCTAGA | 1 cut(s) | 37 |
| XceI | RCATGY | 2 cut(s) | 140, 287 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 789 |
| XspI | CTAG | 4 cut(s) | 38, 174, 876, 1055 |
| Zsp2I | ATGCAT | 1 cut(s) | 451 |