Belongs to the heat shock protein 70 family
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 1659 |
| AasI | GACNNNNNNGTC | 2 cut(s) | 841, 1213 |
| Acc36I | ACCTGC | 1 cut(s) | 1285 |
| AccB7I | CCANNNNNTGG | 3 cut(s) | 1018, 1225, 1755 |
| AccI | GTMKAC | 1 cut(s) | 680 |
| AccII | CGCG | 3 cut(s) | 26, 170, 188 |
| AciI | CCGC | 5 cut(s) | 24, 170, 223, 549, 1137 |
| AclWI | GGATC | 1 cut(s) | 948 |
| AcoI | YGGCCR | 1 cut(s) | 3 |
| AcyI | GRCGYC | 2 cut(s) | 122, 249 |
| AfaI | GTAC | 4 cut(s) | 628, 1006, 1431, 1592 |
| AfiI | CCNNNNNNNGG | 8 cut(s) | 29, 918, 1018, 1225, 1755, 1874, 1913, 1916 |
| AjnI | CCWGG | 4 cut(s) | 180, 301, 1218, 1262 |
| AleI | CACNNNNGTG | 2 cut(s) | 81, 702 |
| Alw21I | GWGCWC | 2 cut(s) | 796, 1614 |
| Alw26I | GTCTC | 4 cut(s) | 258, 469, 643, 1486 |
| AlwI | GGATC | 1 cut(s) | 948 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 1225 |
| AoxI | GGCC | 6 cut(s) | 3, 278, 298, 480, 508, 1504 |
| ApeKI | GCWGC | 7 cut(s) | 57, 73, 356, 545, 1148, 1709, 1862 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 863, 895 |
| AseI | ATTAAT | 1 cut(s) | 321 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 1902 |
| AspS9I | GGNCC | 5 cut(s) | 20, 299, 1021, 1186, 1931 |
| AsuC2I | CCSGG | 2 cut(s) | 1112, 1326 |
| AvaII | GGWCC | 4 cut(s) | 20, 1021, 1186, 1931 |
| BanII | GRGCYC | 2 cut(s) | 796, 1614 |
| BauI | CACGAG | 1 cut(s) | 1368 |
| Bbv12I | GWGCWC | 2 cut(s) | 796, 1614 |
| BbvCI | CCTCAGC | 2 cut(s) | 1488, 1785 |
| BbvI | GCAGC | 7 cut(s) | 44, 85, 343, 532, 1135, 1696, 1849 |
| BccI | CCATC | 5 cut(s) | 721, 988, 1564, 1757, 1841 |
| BceAI | ACGGC | 2 cut(s) | 109, 1124 |
| BciT130I | CCWGG | 4 cut(s) | 182, 303, 1220, 1264 |
| BcnI | CCSGG | 2 cut(s) | 1112, 1326 |
| BcoDI | GTCTC | 4 cut(s) | 258, 469, 643, 1486 |
| BfaI | CTAG | 3 cut(s) | 383, 918, 1041 |
| BfmI | CTRYAG | 3 cut(s) | 357, 1500, 1710 |
| BfuAI | ACCTGC | 1 cut(s) | 1285 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 1145 |
| BmcAI | AGTACT | 1 cut(s) | 1006 |
| Bme1390I | CCNGG | 6 cut(s) | 182, 303, 1112, 1220, 1264, 1326 |
| Bme18I | GGWCC | 4 cut(s) | 20, 1021, 1186, 1931 |
| BmgT120I | GGNCC | 5 cut(s) | 20, 299, 1021, 1186, 1931 |
| BmiI | GGNNCC | 6 cut(s) | 21, 22, 46, 963, 1135, 1933 |
| BmrFI | CCNGG | 6 cut(s) | 182, 303, 1112, 1220, 1264, 1326 |
| BmsI | GCATC | 6 cut(s) | 205, 381, 481, 976, 1450, 1723 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 857, 889 |
| BpmI | CTGGAG | 3 cut(s) | 708, 1257, 1398 |
| Bpu10I | CCTNAGC | 2 cut(s) | 1488, 1785 |
| BpuEI | CTTGAG | 1 cut(s) | 1598 |
| BpuMI | CCSGG | 2 cut(s) | 1112, 1326 |
| Bsa29I | ATCGAT | 2 cut(s) | 36, 1458 |
| BsaBI | GATNNNNATC | 3 cut(s) | 32, 1523, 1767 |
| BsaHI | GRCGYC | 2 cut(s) | 122, 249 |
| BsaJI | CCNNGG | 7 cut(s) | 301, 1218, 1219, 1262, 1421, 1641, 1779 |
| BsaXI | ACNNNNNCTCC | 4 cut(s) | 229, 259, 1125, 1155 |
| Bsc4I | CCNNNNNNNGG | 8 cut(s) | 29, 918, 1018, 1225, 1755, 1874, 1913, 1916 |
| Bse118I | RCCGGY | 2 cut(s) | 296, 964 |
| Bse1I | ACTGG | 1 cut(s) | 582 |
| Bse3DI | GCAATG | 3 cut(s) | 501, 552, 1836 |
| Bse8I | GATNNNNATC | 3 cut(s) | 32, 1523, 1767 |
| BseBI | CCWGG | 4 cut(s) | 182, 303, 1220, 1264 |
| BseCI | ATCGAT | 2 cut(s) | 36, 1458 |
| BseDI | CCNNGG | 7 cut(s) | 301, 1218, 1219, 1262, 1421, 1641, 1779 |
| BseGI | GGATG | 5 cut(s) | 496, 732, 1120, 1768, 1900 |
| BseJI | GATNNNNATC | 3 cut(s) | 32, 1523, 1767 |
| BseLI | CCNNNNNNNGG | 8 cut(s) | 29, 918, 1018, 1225, 1755, 1874, 1913, 1916 |
| BseMI | GCAATG | 3 cut(s) | 501, 552, 1836 |
| BseMII | CTCAG | 8 cut(s) | 141, 485, 866, 971, 1448, 1479, 1575, 1776 |
| BseNI | ACTGG | 1 cut(s) | 582 |
| BseRI | GAGGAG | 3 cut(s) | 1200, 1571, 1622 |
| BseXI | GCAGC | 7 cut(s) | 44, 85, 343, 532, 1135, 1696, 1849 |
| BseYI | CCCAGC | 2 cut(s) | 494, 1384 |
| BsgI | GTGCAG | 2 cut(s) | 1163, 1202 |
| Bsh1236I | CGCG | 3 cut(s) | 26, 170, 188 |
| Bsh1285I | CGRYCG | 2 cut(s) | 30, 82 |
| BshFI | GGCC | 6 cut(s) | 5, 280, 300, 482, 510, 1506 |
| BshVI | ATCGAT | 2 cut(s) | 36, 1458 |
| BsiEI | CGRYCG | 2 cut(s) | 30, 82 |
| BsiHKAI | GWGCWC | 2 cut(s) | 796, 1614 |
| BsiSI | CCGG | 5 cut(s) | 6, 297, 965, 1112, 1326 |
| BslFI | GGGAC | 3 cut(s) | 33, 1201, 1917 |
| BslI | CCNNNNNNNGG | 8 cut(s) | 29, 918, 1018, 1225, 1755, 1874, 1913, 1916 |
| BsmAI | GTCTC | 4 cut(s) | 258, 469, 643, 1486 |
| BsmFI | GGGAC | 3 cut(s) | 33, 1201, 1917 |
| BsmI | GAATGC | 4 cut(s) | 439, 1407, 1470, 1642 |
| BsnI | GGCC | 6 cut(s) | 5, 280, 300, 482, 510, 1506 |
| Bsp1286I | GDGCHC | 2 cut(s) | 796, 1614 |
| Bsp68I | TCGCGA | 1 cut(s) | 188 |
| BspACI | CCGC | 5 cut(s) | 24, 170, 223, 549, 1137 |
| BspANI | GGCC | 6 cut(s) | 5, 280, 300, 482, 510, 1506 |
| BspCNI | CTCAG | 8 cut(s) | 142, 484, 865, 972, 1447, 1480, 1576, 1777 |
| BspDI | ATCGAT | 2 cut(s) | 36, 1458 |
| BspFNI | CGCG | 3 cut(s) | 26, 170, 188 |
| BspLI | GGNNCC | 6 cut(s) | 21, 22, 46, 963, 1135, 1933 |
| BspMAI | CTGCAG | 2 cut(s) | 361, 1714 |
| BspMI | ACCTGC | 1 cut(s) | 1285 |
| BspPI | GGATC | 1 cut(s) | 948 |
| BspQI | GCTCTTC | 1 cut(s) | 921 |
| BsrDI | GCAATG | 3 cut(s) | 501, 552, 1836 |
| BsrFI | RCCGGY | 2 cut(s) | 296, 964 |
| BsrI | ACTGG | 1 cut(s) | 582 |
| BssAI | RCCGGY | 2 cut(s) | 296, 964 |
| BssECI | CCNNGG | 7 cut(s) | 301, 1218, 1219, 1262, 1421, 1641, 1779 |
| BssNI | GRCGYC | 2 cut(s) | 122, 249 |
| BssSI | CACGAG | 1 cut(s) | 1368 |
| BssT1I | CCWWGG | 2 cut(s) | 1421, 1641 |
| Bst2BI | CACGAG | 1 cut(s) | 1368 |
| Bst2UI | CCWGG | 4 cut(s) | 182, 303, 1220, 1264 |
| Bst4CI | ACNGT | 8 cut(s) | 83, 208, 351, 448, 1009, 1095, 1252, 1444 |
| Bst6I | CTCTTC | 2 cut(s) | 336, 921 |
| BstACI | GRCGYC | 2 cut(s) | 122, 249 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 1066 |
| BstC8I | GCNNGC | 6 cut(s) | 298, 453, 508, 1146, 1746, 1776 |
| BstF5I | GGATG | 5 cut(s) | 496, 732, 1120, 1768, 1900 |
| BstFNI | CGCG | 3 cut(s) | 26, 170, 188 |
| BstMAI | GTCTC | 4 cut(s) | 258, 469, 643, 1486 |
| BstMCI | CGRYCG | 2 cut(s) | 30, 82 |
| BstMWI | GCNNNNNNNGC | 4 cut(s) | 554, 1066, 1133, 1145 |
| BstNI | CCWGG | 4 cut(s) | 182, 303, 1220, 1264 |
| BstSCI | CCNGG | 6 cut(s) | 180, 301, 1110, 1218, 1262, 1324 |
| BstSFI | CTRYAG | 3 cut(s) | 357, 1500, 1710 |
| BstUI | CGCG | 3 cut(s) | 26, 170, 188 |
| BstV1I | GCAGC | 7 cut(s) | 44, 85, 343, 532, 1135, 1696, 1849 |
| BstX2I | RGATCY | 1 cut(s) | 940 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 704 |
| BstYI | RGATCY | 1 cut(s) | 940 |
| Bsu15I | ATCGAT | 2 cut(s) | 36, 1458 |
| BsuRI | GGCC | 6 cut(s) | 5, 280, 300, 482, 510, 1506 |
| BsuTUI | ATCGAT | 2 cut(s) | 36, 1458 |
| BtgZI | GCGATG | 2 cut(s) | 79, 203 |
| BtsCI | GGATG | 5 cut(s) | 496, 732, 1120, 1768, 1900 |
| BtsI | GCAGTG | 2 cut(s) | 846, 1927 |
| BtsIMutI | CAGTG | 6 cut(s) | 204, 589, 784, 846, 1440, 1927 |
| BtuMI | TCGCGA | 1 cut(s) | 188 |
| BveI | ACCTGC | 1 cut(s) | 1285 |
| Cac8I | GCNNGC | 6 cut(s) | 298, 453, 508, 1146, 1746, 1776 |
| CaiI | CAGNNNCTG | 1 cut(s) | 1225 |
| Cfr10I | RCCGGY | 2 cut(s) | 296, 964 |
| Cfr13I | GGNCC | 5 cut(s) | 20, 299, 1021, 1186, 1931 |
| ClaI | ATCGAT | 2 cut(s) | 36, 1458 |
| CseI | GACGC | 5 cut(s) | 49, 130, 176, 257, 1715 |
| CsiI | ACCWGGT | 1 cut(s) | 180 |
| Csp6I | GTAC | 4 cut(s) | 627, 1005, 1430, 1591 |
| CviAII | CATG | 7 cut(s) | 268, 316, 937, 1013, 1663, 1873, 1891 |
| CviQI | GTAC | 4 cut(s) | 627, 1005, 1430, 1591 |
| DrdI | GACNNNNNNGTC | 2 cut(s) | 841, 1213 |
| DseDI | GACNNNNNNGTC | 2 cut(s) | 841, 1213 |
| EaeI | YGGCCR | 1 cut(s) | 3 |
| Eam1104I | CTCTTC | 2 cut(s) | 336, 921 |
| EarI | CTCTTC | 2 cut(s) | 336, 921 |
| Ecl136II | GAGCTC | 2 cut(s) | 794, 1612 |
| Eco130I | CCWWGG | 2 cut(s) | 1421, 1641 |
| Eco24I | GRGCYC | 2 cut(s) | 796, 1614 |
| Eco47I | GGWCC | 4 cut(s) | 20, 1021, 1186, 1931 |
| Eco53kI | GAGCTC | 2 cut(s) | 794, 1612 |
| EcoICRI | GAGCTC | 2 cut(s) | 794, 1612 |
| EcoO109I | RGGNCCY | 2 cut(s) | 20, 1186 |
| EcoRII | CCWGG | 4 cut(s) | 180, 301, 1218, 1262 |
| EcoT14I | CCWWGG | 2 cut(s) | 1421, 1641 |
| EcoT38I | GRGCYC | 2 cut(s) | 796, 1614 |
| ErhI | CCWWGG | 2 cut(s) | 1421, 1641 |
| FaeI | CATG | 7 cut(s) | 271, 319, 940, 1016, 1666, 1876, 1894 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 1625, 1657 |
| FaqI | GGGAC | 3 cut(s) | 33, 1201, 1917 |
| FatI | CATG | 7 cut(s) | 267, 315, 936, 1012, 1662, 1872, 1890 |
| FauI | CCCGC | 1 cut(s) | 31 |
| FauNDI | CATATG | 1 cut(s) | 559 |
| FblI | GTMKAC | 1 cut(s) | 680 |
| FokI | GGATG | 5 cut(s) | 503, 739, 1127, 1775, 1907 |
| FriOI | GRGCYC | 2 cut(s) | 796, 1614 |
| FspBI | CTAG | 3 cut(s) | 383, 918, 1041 |
| GsaI | CCCAGC | 2 cut(s) | 498, 1388 |
| GsuI | CTGGAG | 3 cut(s) | 708, 1257, 1398 |
| HaeIII | GGCC | 6 cut(s) | 5, 280, 300, 482, 510, 1506 |
| HapII | CCGG | 5 cut(s) | 6, 297, 965, 1112, 1326 |
| HgaI | GACGC | 5 cut(s) | 49, 130, 176, 257, 1715 |
| Hin1I | GRCGYC | 2 cut(s) | 122, 249 |
| Hin1II | CATG | 7 cut(s) | 271, 319, 940, 1016, 1666, 1876, 1894 |
| HindIII | AAGCTT | 1 cut(s) | 1700 |
| HpaII | CCGG | 5 cut(s) | 6, 297, 965, 1112, 1326 |
| Hpy166II | GTNNAC | 3 cut(s) | 141, 681, 846 |
| Hpy8I | GTNNAC | 3 cut(s) | 141, 681, 846 |
| Hpy99I | CGWCG | 2 cut(s) | 65, 124 |
| HpyAV | CCTTC | 7 cut(s) | 568, 653, 1171, 1536, 1615, 1801, 1811 |
| HpyCH4III | ACNGT | 8 cut(s) | 83, 208, 351, 448, 1009, 1095, 1252, 1444 |
| HpyF10VI | GCNNNNNNNGC | 4 cut(s) | 554, 1066, 1133, 1145 |
| Hsp92I | GRCGYC | 2 cut(s) | 122, 249 |
| Hsp92II | CATG | 7 cut(s) | 271, 319, 940, 1016, 1666, 1876, 1894 |
| KflI | GGGWCCC | 1 cut(s) | 20 |
| KroI | GCCGGC | 1 cut(s) | 296 |
| KroNI | GCCGGC | 1 cut(s) | 298 |
| LguI | GCTCTTC | 1 cut(s) | 921 |
| LmnI | GCTCC | 6 cut(s) | 961, 1133, 1291, 1609, 1810, 1870 |
| Lsp1109I | GCAGC | 7 cut(s) | 44, 85, 343, 532, 1135, 1696, 1849 |
| LweI | GCATC | 6 cut(s) | 205, 381, 481, 976, 1450, 1723 |
| MabI | ACCWGGT | 1 cut(s) | 180 |
| MaeI | CTAG | 3 cut(s) | 383, 918, 1041 |
| MaeIII | GTNAC | 6 cut(s) | 164, 245, 305, 325, 442, 1204 |
| MfeI | CAATTG | 2 cut(s) | 651, 1737 |
| MflI | RGATCY | 1 cut(s) | 940 |
| MhlI | GDGCHC | 2 cut(s) | 796, 1614 |
| MluCI | AATT | 5 cut(s) | 228, 426, 651, 1275, 1737 |
| MlyI | GAGTC | 5 cut(s) | 461, 829, 866, 1201, 1360 |
| MmeI | TCCRAC | 2 cut(s) | 43, 1304 |
| MroNI | GCCGGC | 1 cut(s) | 296 |
| MroXI | GAANNNNTTC | 1 cut(s) | 1902 |
| MseI | TTAA | 6 cut(s) | 321, 429, 513, 933, 1107, 1954 |
| MslI | CAYNNNNRTG | 3 cut(s) | 81, 702, 1877 |
| MspA1I | CMGCKG | 1 cut(s) | 57 |
| MspI | CCGG | 5 cut(s) | 6, 297, 965, 1112, 1326 |
| MspR9I | CCNGG | 6 cut(s) | 182, 303, 1112, 1220, 1264, 1326 |
| MunI | CAATTG | 2 cut(s) | 651, 1737 |
| Mva1269I | GAATGC | 4 cut(s) | 439, 1407, 1470, 1642 |
| MvaI | CCWGG | 4 cut(s) | 182, 303, 1220, 1264 |
| MvnI | CGCG | 3 cut(s) | 26, 170, 188 |
| MwoI | GCNNNNNNNGC | 4 cut(s) | 554, 1066, 1133, 1145 |
| NaeI | GCCGGC | 1 cut(s) | 298 |
| NciI | CCSGG | 2 cut(s) | 1112, 1326 |
| NdeI | CATATG | 1 cut(s) | 559 |
| NgoMIV | GCCGGC | 1 cut(s) | 296 |
| NlaIII | CATG | 7 cut(s) | 271, 319, 940, 1016, 1666, 1876, 1894 |
| NlaIV | GGNNCC | 6 cut(s) | 21, 22, 46, 963, 1135, 1933 |
| NmeAIII | GCCGAG | 1 cut(s) | 1804 |
| NmuCI | GTSAC | 4 cut(s) | 164, 245, 305, 1204 |
| NruI | TCGCGA | 1 cut(s) | 188 |
| OliI | CACNNNNGTG | 2 cut(s) | 81, 702 |
| PasI | CCCWGGG | 1 cut(s) | 1219 |
| PciSI | GCTCTTC | 1 cut(s) | 921 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 84 |
| PctI | GAATGC | 4 cut(s) | 439, 1407, 1470, 1642 |
| PdiI | GCCGGC | 1 cut(s) | 298 |
| PdmI | GAANNNNTTC | 1 cut(s) | 1902 |
| PfeI | GAWTC | 7 cut(s) | 33, 733, 1045, 1258, 1336, 1628, 1768 |
| PflFI | GACNNNGTC | 2 cut(s) | 83, 124 |
| PflMI | CCANNNNNTGG | 3 cut(s) | 1018, 1225, 1755 |
| Ple19I | CGATCG | 1 cut(s) | 30 |
| PleI | GAGTC | 5 cut(s) | 461, 829, 866, 1201, 1360 |
| PpsI | GAGTC | 5 cut(s) | 461, 829, 866, 1201, 1360 |
| PpuMI | RGGWCCY | 2 cut(s) | 20, 1186 |
| PshBI | ATTAAT | 1 cut(s) | 321 |
| PsiI | TTATAA | 1 cut(s) | 1659 |
| Psp124BI | GAGCTC | 2 cut(s) | 796, 1614 |
| Psp5II | RGGWCCY | 2 cut(s) | 20, 1186 |
| Psp6I | CCWGG | 4 cut(s) | 180, 301, 1218, 1262 |
| PspFI | CCCAGC | 2 cut(s) | 494, 1384 |
| PspGI | CCWGG | 4 cut(s) | 180, 301, 1218, 1262 |
| PspN4I | GGNNCC | 6 cut(s) | 21, 22, 46, 963, 1135, 1933 |
| PspPI | GGNCC | 5 cut(s) | 20, 299, 1021, 1186, 1931 |
| PspPPI | RGGWCCY | 2 cut(s) | 20, 1186 |
| PstI | CTGCAG | 2 cut(s) | 361, 1714 |
| PstNI | CAGNNNCTG | 1 cut(s) | 1225 |
| PsuI | RGATCY | 1 cut(s) | 940 |
| PsyI | GACNNNGTC | 2 cut(s) | 83, 124 |
| PvuI | CGATCG | 1 cut(s) | 30 |
| PvuII | CAGCTG | 1 cut(s) | 57 |
| RruI | TCGCGA | 1 cut(s) | 188 |
| RsaI | GTAC | 4 cut(s) | 628, 1006, 1431, 1592 |
| RsaNI | GTAC | 4 cut(s) | 627, 1005, 1430, 1591 |
| RseI | CAYNNNNRTG | 3 cut(s) | 81, 702, 1877 |
| SacI | GAGCTC | 2 cut(s) | 796, 1614 |
| SapI | GCTCTTC | 1 cut(s) | 921 |
| SaqAI | TTAA | 6 cut(s) | 321, 429, 513, 933, 1107, 1954 |
| Sau96I | GGNCC | 5 cut(s) | 20, 299, 1021, 1186, 1931 |
| ScaI | AGTACT | 1 cut(s) | 1006 |
| SchI | GAGTC | 5 cut(s) | 461, 829, 866, 1201, 1360 |
| ScrFI | CCNGG | 6 cut(s) | 182, 303, 1112, 1220, 1264, 1326 |
| SduI | GDGCHC | 2 cut(s) | 796, 1614 |
| SexAI | ACCWGGT | 1 cut(s) | 180 |
| SfaNI | GCATC | 6 cut(s) | 205, 381, 481, 976, 1450, 1723 |
| SfcI | CTRYAG | 3 cut(s) | 357, 1500, 1710 |
| SinI | GGWCC | 4 cut(s) | 20, 1021, 1186, 1931 |
| SmiMI | CAYNNNNRTG | 3 cut(s) | 81, 702, 1877 |
| SmlI | CTYRAG | 1 cut(s) | 1613 |
| SmoI | CTYRAG | 1 cut(s) | 1613 |
| Sse9I | AATT | 5 cut(s) | 228, 426, 651, 1275, 1737 |
| SsiI | CCGC | 5 cut(s) | 24, 170, 223, 549, 1137 |
| SspMI | CTAG | 3 cut(s) | 383, 918, 1041 |
| SstI | GAGCTC | 2 cut(s) | 796, 1614 |
| StyD4I | CCNGG | 6 cut(s) | 180, 301, 1110, 1218, 1262, 1324 |
| StyI | CCWWGG | 2 cut(s) | 1421, 1641 |
| TaaI | ACNGT | 8 cut(s) | 83, 208, 351, 448, 1009, 1095, 1252, 1444 |
| TaqI | TCGA | 8 cut(s) | 36, 87, 213, 612, 891, 1458, 1563, 1941 |
| TasI | AATT | 5 cut(s) | 228, 426, 651, 1275, 1737 |
| TatI | WGTACW | 2 cut(s) | 1004, 1590 |
| TauI | GCSGC | 4 cut(s) | 173, 226, 551, 1139 |
| TfiI | GAWTC | 7 cut(s) | 33, 733, 1045, 1258, 1336, 1628, 1768 |
| Tru1I | TTAA | 6 cut(s) | 321, 429, 513, 933, 1107, 1954 |
| Tru9I | TTAA | 6 cut(s) | 321, 429, 513, 933, 1107, 1954 |
| TscAI | CASTG | 6 cut(s) | 211, 589, 784, 853, 1447, 1927 |
| TseFI | GTSAC | 4 cut(s) | 164, 245, 305, 1204 |
| TseI | GCWGC | 7 cut(s) | 57, 73, 356, 545, 1148, 1709, 1862 |
| Tsp45I | GTSAC | 4 cut(s) | 164, 245, 305, 1204 |
| TspDTI | ATGAA | 4 cut(s) | 206, 284, 1131, 1911 |
| TspRI | CASTG | 6 cut(s) | 211, 589, 784, 853, 1447, 1927 |
| Tth111I | GACNNNGTC | 2 cut(s) | 83, 124 |
| Van91I | CCANNNNNTGG | 3 cut(s) | 1018, 1225, 1755 |
| VpaK11BI | GGWCC | 4 cut(s) | 20, 1021, 1186, 1931 |
| VspI | ATTAAT | 1 cut(s) | 321 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 1756 |
| XmiI | GTMKAC | 1 cut(s) | 680 |
| XmnI | GAANNNNTTC | 1 cut(s) | 1902 |
| XspI | CTAG | 3 cut(s) | 383, 918, 1041 |
| ZrmI | AGTACT | 1 cut(s) | 1006 |