Heat shock cognate 70 kDa
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 440 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 29 |
| AccBSI | CCGCTC | 1 cut(s) | 1158 |
| AciI | CCGC | 5 cut(s) | 171, 223, 1158, 1281, 1487 |
| AclWI | GGATC | 4 cut(s) | 242, 424, 945, 1039 |
| AcsI | RAATTY | 4 cut(s) | 660, 917, 1041, 1470 |
| AfaI | GTAC | 6 cut(s) | 625, 1020, 1053, 1140, 1346, 1390 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 29, 258 |
| AgsI | TTSAA | 4 cut(s) | 286, 863, 881, 1078 |
| AjnI | CCWGG | 1 cut(s) | 908 |
| AloI | GAACNNNNNNTCC | 2 cut(s) | 582, 614 |
| AluBI | AGCT | 9 cut(s) | 98, 545, 677, 928, 1090, 1136, 1145, 1399, 1498 |
| AluI | AGCT | 9 cut(s) | 98, 545, 677, 928, 1090, 1136, 1145, 1399, 1498 |
| Alw21I | GWGCWC | 1 cut(s) | 930 |
| Alw26I | GTCTC | 2 cut(s) | 190, 1486 |
| AlwI | GGATC | 4 cut(s) | 242, 424, 945, 1039 |
| AoxI | GGCC | 2 cut(s) | 278, 405 |
| ApeKI | GCWGC | 6 cut(s) | 5, 353, 542, 545, 1133, 1145 |
| ApoI | RAATTY | 4 cut(s) | 660, 917, 1041, 1470 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 835 |
| AsuHPI | GGTGA | 6 cut(s) | 176, 682, 716, 739, 1172, 1364 |
| BanII | GRGCYC | 1 cut(s) | 930 |
| Bbv12I | GWGCWC | 1 cut(s) | 930 |
| BbvCI | CCTCAGC | 1 cut(s) | 357 |
| BbvI | GCAGC | 5 cut(s) | 340, 532, 554, 1132, 1145 |
| BccI | CCATC | 2 cut(s) | 1283, 1567 |
| BceAI | ACGGC | 1 cut(s) | 555 |
| BciT130I | CCWGG | 1 cut(s) | 910 |
| BcoDI | GTCTC | 2 cut(s) | 190, 1486 |
| BfaI | CTAG | 1 cut(s) | 1038 |
| BfmI | CTRYAG | 2 cut(s) | 681, 1537 |
| BisI | GCNGC | 7 cut(s) | 6, 171, 354, 543, 546, 1134, 1146 |
| BlsI | GCNGC | 7 cut(s) | 7, 172, 355, 544, 547, 1135, 1147 |
| Bme1390I | CCNGG | 1 cut(s) | 910 |
| BmiI | GGNNCC | 1 cut(s) | 960 |
| BmrFI | CCNGG | 1 cut(s) | 910 |
| BmsI | GCATC | 6 cut(s) | 157, 205, 478, 973, 1110, 1416 |
| Bpu10I | CCTNAGC | 1 cut(s) | 357 |
| BpuEI | CTTGAG | 1 cut(s) | 629 |
| BsaAI | YACGTR | 1 cut(s) | 521 |
| BsaBI | GATNNNNATC | 2 cut(s) | 693, 1523 |
| BsaJI | CCNNGG | 2 cut(s) | 106, 909 |
| BsaXI | ACNNNNNCTCC | 4 cut(s) | 332, 362, 881, 911 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 29, 258 |
| Bse118I | RCCGGY | 2 cut(s) | 503, 961 |
| Bse3DI | GCAATG | 1 cut(s) | 549 |
| Bse8I | GATNNNNATC | 2 cut(s) | 693, 1523 |
| BseBI | CCWGG | 1 cut(s) | 910 |
| BseDI | CCNNGG | 2 cut(s) | 106, 909 |
| BseGI | GGATG | 5 cut(s) | 1207, 1255, 1275, 1407, 1578 |
| BseJI | GATNNNNATC | 2 cut(s) | 693, 1523 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 29, 258 |
| BseMI | GCAATG | 1 cut(s) | 549 |
| BseMII | CTCAG | 3 cut(s) | 348, 794, 1416 |
| BseRI | GAGGAG | 1 cut(s) | 938 |
| BseXI | GCAGC | 5 cut(s) | 340, 532, 554, 1132, 1145 |
| BsgI | GTGCAG | 1 cut(s) | 1152 |
| Bsh1285I | CGRYCG | 1 cut(s) | 966 |
| BshFI | GGCC | 2 cut(s) | 280, 407 |
| BsiEI | CGRYCG | 1 cut(s) | 966 |
| BsiHKAI | GWGCWC | 1 cut(s) | 930 |
| BsiSI | CCGG | 3 cut(s) | 504, 962, 1433 |
| BslFI | GGGAC | 1 cut(s) | 317 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 29, 258 |
| BsmAI | GTCTC | 2 cut(s) | 190, 1486 |
| BsmFI | GGGAC | 1 cut(s) | 317 |
| BsnI | GGCC | 2 cut(s) | 280, 407 |
| Bsp1286I | GDGCHC | 1 cut(s) | 930 |
| Bsp1407I | TGTACA | 1 cut(s) | 1138 |
| Bsp143I | GATC | 8 cut(s) | 37, 90, 247, 416, 610, 937, 1031, 1518 |
| BspACI | CCGC | 5 cut(s) | 171, 223, 1158, 1281, 1487 |
| BspANI | GGCC | 2 cut(s) | 280, 407 |
| BspCNI | CTCAG | 3 cut(s) | 349, 795, 1415 |
| BspHI | TCATGA | 1 cut(s) | 1009 |
| BspLI | GGNNCC | 1 cut(s) | 960 |
| BspMAI | CTGCAG | 1 cut(s) | 685 |
| BspPI | GGATC | 4 cut(s) | 242, 424, 945, 1039 |
| BsrBI | CCGCTC | 1 cut(s) | 1158 |
| BsrDI | GCAATG | 1 cut(s) | 549 |
| BsrFI | RCCGGY | 2 cut(s) | 503, 961 |
| BsrGI | TGTACA | 1 cut(s) | 1138 |
| BssAI | RCCGGY | 2 cut(s) | 503, 961 |
| BssECI | CCNNGG | 2 cut(s) | 106, 909 |
| BssMI | GATC | 8 cut(s) | 37, 90, 247, 416, 610, 937, 1031, 1518 |
| BssT1I | CCWWGG | 1 cut(s) | 106 |
| Bst2UI | CCWGG | 1 cut(s) | 910 |
| Bst4CI | ACNGT | 5 cut(s) | 80, 126, 208, 424, 445 |
| Bst6I | CTCTTC | 1 cut(s) | 645 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 1130 |
| BstAUI | TGTACA | 1 cut(s) | 1138 |
| BstBAI | YACGTR | 1 cut(s) | 521 |
| BstC8I | GCNNGC | 1 cut(s) | 450 |
| BstDEI | CTNAG | 6 cut(s) | 174, 357, 794, 803, 1402, 1554 |
| BstEII | GGTNACC | 1 cut(s) | 727 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 256 |
| BstF5I | GGATG | 5 cut(s) | 1207, 1255, 1275, 1407, 1578 |
| BstKTI | GATC | 8 cut(s) | 40, 93, 250, 419, 613, 940, 1034, 1521 |
| BstMAI | GTCTC | 2 cut(s) | 190, 1486 |
| BstMBI | GATC | 8 cut(s) | 37, 90, 247, 416, 610, 937, 1031, 1518 |
| BstMCI | CGRYCG | 1 cut(s) | 966 |
| BstMWI | GCNNNNNNNGC | 4 cut(s) | 551, 1130, 1142, 1280 |
| BstNI | CCWGG | 1 cut(s) | 910 |
| BstPI | GGTNACC | 1 cut(s) | 727 |
| BstSCI | CCNGG | 1 cut(s) | 908 |
| BstSFI | CTRYAG | 2 cut(s) | 681, 1537 |
| BstSNI | TACGTA | 1 cut(s) | 521 |
| BstV1I | GCAGC | 5 cut(s) | 340, 532, 554, 1132, 1145 |
| BstX2I | RGATCY | 2 cut(s) | 937, 1031 |
| BstYI | RGATCY | 2 cut(s) | 937, 1031 |
| BsuRI | GGCC | 2 cut(s) | 280, 407 |
| BtsCI | GGATG | 5 cut(s) | 1207, 1255, 1275, 1407, 1578 |
| BtsI | GCAGTG | 1 cut(s) | 1269 |
| BtsIMutI | CAGTG | 2 cut(s) | 204, 1269 |
| Cac8I | GCNNGC | 1 cut(s) | 450 |
| CciI | TCATGA | 1 cut(s) | 1009 |
| Cfr10I | RCCGGY | 2 cut(s) | 503, 961 |
| Csp6I | GTAC | 6 cut(s) | 624, 1019, 1052, 1139, 1345, 1389 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 274, 309 |
| CviAII | CATG | 6 cut(s) | 57, 268, 373, 934, 955, 1010 |
| CviQI | GTAC | 6 cut(s) | 624, 1019, 1052, 1139, 1345, 1389 |
| DdeI | CTNAG | 6 cut(s) | 174, 357, 794, 803, 1402, 1554 |
| DpnI | GATC | 8 cut(s) | 39, 92, 249, 418, 612, 939, 1033, 1520 |
| DpnII | GATC | 8 cut(s) | 37, 90, 247, 416, 610, 937, 1031, 1518 |
| DraI | TTTAAA | 1 cut(s) | 385 |
| Eam1104I | CTCTTC | 1 cut(s) | 645 |
| EarI | CTCTTC | 1 cut(s) | 645 |
| Ecl136II | GAGCTC | 1 cut(s) | 928 |
| Eco105I | TACGTA | 1 cut(s) | 521 |
| Eco130I | CCWWGG | 1 cut(s) | 106 |
| Eco147I | AGGCCT | 1 cut(s) | 407 |
| Eco24I | GRGCYC | 1 cut(s) | 930 |
| Eco32I | GATATC | 1 cut(s) | 1438 |
| Eco53kI | GAGCTC | 1 cut(s) | 928 |
| Eco91I | GGTNACC | 1 cut(s) | 727 |
| EcoICRI | GAGCTC | 1 cut(s) | 928 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 256 |
| EcoO65I | GGTNACC | 1 cut(s) | 727 |
| EcoRI | GAATTC | 1 cut(s) | 1041 |
| EcoRII | CCWGG | 1 cut(s) | 908 |
| EcoRV | GATATC | 1 cut(s) | 1438 |
| EcoT14I | CCWWGG | 1 cut(s) | 106 |
| EcoT22I | ATGCAT | 1 cut(s) | 956 |
| EcoT38I | GRGCYC | 1 cut(s) | 930 |
| ErhI | CCWWGG | 1 cut(s) | 106 |
| FaeI | CATG | 6 cut(s) | 60, 271, 376, 937, 958, 1013 |
| FaqI | GGGAC | 1 cut(s) | 317 |
| FatI | CATG | 6 cut(s) | 56, 267, 372, 933, 954, 1009 |
| FauI | CCCGC | 1 cut(s) | 1288 |
| FauNDI | CATATG | 1 cut(s) | 1126 |
| Fnu4HI | GCNGC | 7 cut(s) | 6, 171, 354, 543, 546, 1134, 1146 |
| FokI | GGATG | 5 cut(s) | 1214, 1262, 1262, 1394, 1585 |
| FriOI | GRGCYC | 1 cut(s) | 930 |
| Fsp4HI | GCNGC | 7 cut(s) | 6, 171, 354, 543, 546, 1134, 1146 |
| FspBI | CTAG | 1 cut(s) | 1038 |
| GluI | GCNGC | 7 cut(s) | 6, 171, 354, 543, 546, 1134, 1146 |
| HaeIII | GGCC | 2 cut(s) | 280, 407 |
| HapII | CCGG | 3 cut(s) | 504, 962, 1433 |
| Hin1II | CATG | 6 cut(s) | 60, 271, 376, 937, 958, 1013 |
| HincII | GTYRAC | 1 cut(s) | 325 |
| HindII | GTYRAC | 1 cut(s) | 325 |
| HinfI | GANTC | 3 cut(s) | 178, 376, 869 |
| HpaI | GTTAAC | 1 cut(s) | 325 |
| HpaII | CCGG | 3 cut(s) | 504, 962, 1433 |
| HphI | GGTGA | 6 cut(s) | 176, 682, 716, 739, 1172, 1364 |
| Hpy166II | GTNNAC | 3 cut(s) | 325, 1319, 1345 |
| Hpy188I | TCNGA | 2 cut(s) | 44, 416 |
| Hpy188III | TCNNGA | 6 cut(s) | 646, 1010, 1109, 1184, 1528, 1567 |
| Hpy8I | GTNNAC | 3 cut(s) | 325, 1319, 1345 |
| HpyAV | CCTTC | 6 cut(s) | 650, 792, 875, 1157, 1171, 1286 |
| HpyCH4III | ACNGT | 5 cut(s) | 80, 126, 208, 424, 445 |
| HpyCH4IV | ACGT | 2 cut(s) | 84, 520 |
| HpyCH4V | TGCA | 9 cut(s) | 8, 218, 683, 954, 1095, 1124, 1133, 1148, 1418 |
| HpyF10VI | GCNNNNNNNGC | 4 cut(s) | 551, 1130, 1142, 1280 |
| HpyF3I | CTNAG | 6 cut(s) | 174, 357, 794, 803, 1402, 1554 |
| HpySE526I | ACGT | 2 cut(s) | 84, 520 |
| Hsp92II | CATG | 6 cut(s) | 60, 271, 376, 937, 958, 1013 |
| KspAI | GTTAAC | 1 cut(s) | 325 |
| Kzo9I | GATC | 8 cut(s) | 37, 90, 247, 416, 610, 937, 1031, 1518 |
| LmnI | GCTCC | 3 cut(s) | 925, 958, 1087 |
| Lsp1109I | GCAGC | 5 cut(s) | 340, 532, 554, 1132, 1145 |
| LweI | GCATC | 6 cut(s) | 157, 205, 478, 973, 1110, 1416 |
| MaeI | CTAG | 1 cut(s) | 1038 |
| MaeII | ACGT | 2 cut(s) | 84, 520 |
| MaeIII | GTNAC | 5 cut(s) | 80, 263, 727, 776, 1204 |
| MalI | GATC | 8 cut(s) | 39, 92, 249, 418, 612, 939, 1033, 1520 |
| MbiI | CCGCTC | 1 cut(s) | 1158 |
| MboI | GATC | 8 cut(s) | 37, 90, 247, 416, 610, 937, 1031, 1518 |
| MboII | GAAGA | 5 cut(s) | 355, 360, 632, 719, 831 |
| MfeI | CAATTG | 1 cut(s) | 579 |
| MflI | RGATCY | 2 cut(s) | 937, 1031 |
| MhlI | GDGCHC | 1 cut(s) | 930 |
| MmeI | TCCRAC | 1 cut(s) | 43 |
| Mph1103I | ATGCAT | 1 cut(s) | 956 |
| MroXI | GAANNNNTTC | 1 cut(s) | 835 |
| MseI | TTAA | 7 cut(s) | 324, 384, 459, 564, 750, 1395, 1421 |
| MspA1I | CMGCKG | 2 cut(s) | 545, 1136 |
| MspI | CCGG | 3 cut(s) | 504, 962, 1433 |
| MspR9I | CCNGG | 1 cut(s) | 910 |
| MunI | CAATTG | 1 cut(s) | 579 |
| MvaI | CCWGG | 1 cut(s) | 910 |
| MwoI | GCNNNNNNNGC | 4 cut(s) | 551, 1130, 1142, 1280 |
| NdeI | CATATG | 1 cut(s) | 1126 |
| NdeII | GATC | 8 cut(s) | 37, 90, 247, 416, 610, 937, 1031, 1518 |
| NlaIII | CATG | 6 cut(s) | 60, 271, 376, 937, 958, 1013 |
| NlaIV | GGNNCC | 1 cut(s) | 960 |
| NmuCI | GTSAC | 4 cut(s) | 80, 263, 727, 1204 |
| NsiI | ATGCAT | 1 cut(s) | 956 |
| PagI | TCATGA | 1 cut(s) | 1009 |
| PceI | AGGCCT | 1 cut(s) | 407 |
| PdmI | GAANNNNTTC | 1 cut(s) | 835 |
| PfeI | GAWTC | 3 cut(s) | 178, 376, 869 |
| PflFI | GACNNNGTC | 1 cut(s) | 124 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 29 |
| PkrI | GCNGC | 7 cut(s) | 7, 172, 355, 544, 547, 1135, 1147 |
| Ppu21I | YACGTR | 1 cut(s) | 521 |
| PsiI | TTATAA | 1 cut(s) | 440 |
| Psp124BI | GAGCTC | 1 cut(s) | 930 |
| Psp6I | CCWGG | 1 cut(s) | 908 |
| PspEI | GGTNACC | 1 cut(s) | 727 |
| PspGI | CCWGG | 1 cut(s) | 908 |
| PspN4I | GGNNCC | 1 cut(s) | 960 |
| PstI | CTGCAG | 1 cut(s) | 685 |
| PsuI | RGATCY | 2 cut(s) | 937, 1031 |
| PsyI | GACNNNGTC | 1 cut(s) | 124 |
| PvuII | CAGCTG | 2 cut(s) | 545, 1136 |
| RsaI | GTAC | 6 cut(s) | 625, 1020, 1053, 1140, 1346, 1390 |
| RsaNI | GTAC | 6 cut(s) | 624, 1019, 1052, 1139, 1345, 1389 |
| SacI | GAGCTC | 1 cut(s) | 930 |
| SaqAI | TTAA | 7 cut(s) | 324, 384, 459, 564, 750, 1395, 1421 |
| SatI | GCNGC | 7 cut(s) | 6, 171, 354, 543, 546, 1134, 1146 |
| Sau3AI | GATC | 8 cut(s) | 37, 90, 247, 416, 610, 937, 1031, 1518 |
| ScrFI | CCNGG | 1 cut(s) | 910 |
| SduI | GDGCHC | 1 cut(s) | 930 |
| SfaNI | GCATC | 6 cut(s) | 157, 205, 478, 973, 1110, 1416 |
| SfcI | CTRYAG | 2 cut(s) | 681, 1537 |
| SmlI | CTYRAG | 1 cut(s) | 644 |
| SmoI | CTYRAG | 1 cut(s) | 644 |
| SnaBI | TACGTA | 1 cut(s) | 521 |
| SseBI | AGGCCT | 1 cut(s) | 407 |
| SsiI | CCGC | 5 cut(s) | 171, 223, 1158, 1281, 1487 |
| SspI | AATATT | 1 cut(s) | 1171 |
| SspMI | CTAG | 1 cut(s) | 1038 |
| SstI | GAGCTC | 1 cut(s) | 930 |
| StuI | AGGCCT | 1 cut(s) | 407 |
| StyD4I | CCNGG | 1 cut(s) | 908 |
| StyI | CCWWGG | 1 cut(s) | 106 |
| TaaI | ACNGT | 5 cut(s) | 80, 126, 208, 424, 445 |
| TaiI | ACGT | 2 cut(s) | 87, 523 |
| TaqI | TCGA | 2 cut(s) | 966, 1566 |
| TatI | WGTACW | 3 cut(s) | 1018, 1051, 1138 |
| TauI | GCSGC | 1 cut(s) | 173 |
| TfiI | GAWTC | 3 cut(s) | 178, 376, 869 |
| Tru1I | TTAA | 7 cut(s) | 324, 384, 459, 564, 750, 1395, 1421 |
| Tru9I | TTAA | 7 cut(s) | 324, 384, 459, 564, 750, 1395, 1421 |
| TscAI | CASTG | 2 cut(s) | 211, 1276 |
| TseFI | GTSAC | 4 cut(s) | 80, 263, 727, 1204 |
| TseI | GCWGC | 6 cut(s) | 5, 353, 542, 545, 1133, 1145 |
| Tsp45I | GTSAC | 4 cut(s) | 80, 263, 727, 1204 |
| TspDTI | ATGAA | 5 cut(s) | 284, 524, 669, 727, 998 |
| TspGWI | ACGGA | 2 cut(s) | 777, 1400 |
| TspRI | CASTG | 2 cut(s) | 211, 1276 |
| Tth111I | GACNNNGTC | 1 cut(s) | 124 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 29 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 256 |
| XapI | RAATTY | 4 cut(s) | 660, 917, 1041, 1470 |
| XmnI | GAANNNNTTC | 1 cut(s) | 835 |
| XspI | CTAG | 1 cut(s) | 1038 |
| Zsp2I | ATGCAT | 1 cut(s) | 956 |