FvH4_1g10791

GDSL esterase lipase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0000448
Physical Location & Seq
Forward (+)
5896373 .. 5898105
1733 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g10791.t1

Sequence Viewer

Length: 1098 bp
ATGGCCAAGTCCGCAATATTTTCTCTTTGGCTTAATATCATTGTGTATCTCGGAATACTTGGATCGCACGTCTCACACTCTCGGCCAGTTGTGCCAGCACTTTACATATTTGGTGATTCTACAGCCGATGTTGGAACCAATGATTATTTGCCCAATTCTAGAGCGAGGGCCGATTTCCCTTTTAACGGCATCGATTTCCCTCAATCCAAACCAACTGGAAGATTTAGCAACGGTTACAACACTATCGATCTTCTAGCCCAGCAACTGCACTTCCATCAAAGCCCCCCGCCTTATCTCTCATTTCTGGACAGCAATATATCGCAATTTCTCATTAGGAATGTTCCAACTAAGGGAATCAACTTCGCTTCGGGAGGGTCAGGAATTCTGCGTTCTACAGGACATAAGTGGGTAAGGGTGATTTCTTTTGGAGAACAGGTCCAACAATTTTCAAGTGTTTGCAGCAAAATTTCAGAACTACTGGGTCCGAATGCCATCGCTAATATCTCCAAGTCTCTCTTTATCATCAGTGTTGGAAGCAATGACATCTTCGAACATTTTGATGCTAAACGCACTGGGCGAAAATACATCCCCACACTTATCTCCACCTACGAGACTCATCTAAGGAAGTTATATAAGCTAGGAGCCAGAAAATTTGGGATAATTAGTGTTGGGGCAATCGGGTGCTGTCCGATCCAACGTAAATCGAATAAATCTGGGGACTGTAAGGAGGCCATGAATGTGGATGCTCAGTTGTTCTATTCTGCACTCCGAGGCCTCTTGCAAAAGTTGAGTTCTGAGTGCAAAGGGTTGAAGTATGCACTTGCGGATGCATACAAACTGACCAGACTTATTATTGACCACCCCGAGAGACCTGTTTTTAAAGAGATGAAAAGAGCTTGCTGTGGAAACGGAACGTTAAACGCAGACGAGCCGTGTAAAAAGGATTCAGTTGTGTGTGGAGATCGCAACACCTACTTGTTTTGGGACCAATACCATCCAACCCAGCAGGCTTCTTATCTCGCAGCCTATAGTCTTTACCATGGCTCCCCACCTTACGTGGAGCCGATGAGTTTTGGAAACTTGGCCCTTCTGCCGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

366

Amino Acids

40.44

Weight (kDa)

9.21

Isoelectric Point (pI)

37.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 35 - 340 1.1e-27 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000448)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g10790 FvH4_1g10790 FvH4_1g10790 FvH4_1g10791 FvH4_1g10800 FvH4_2g15970 FvH4_2g15980 FvH4_2g15990
malus_domestica MD02G1121900.v1.1 MD05G1128700.v1.1 MD10G1131900.v1.1 MD15G1235500.v1.1 MD15G1235600.v1.1
prunus_persica Prupe.7G177500_v2.0.a1 Prupe.7G177600_v2.0.a1 Prupe.8G172800_v2.0.a1
pyrus_communis pycom05g12460 pycom05g12470 pycom10g11300
rosa_chinensis RchiOBHm_Chr2g0097971 RchiOBHm_Chr2g0098021 RchiOBHm_Chr2g0098061 RchiOBHm_Chr6g0257301 RchiOBHm_Chr6g0279511 RchiOBHm_Chr6g0279521
rosa_laevigata RLG00000013139 RLG00000013140 RLG00000013141 RLG00000016747 RLG00000016749 RLG00000016750 RLG00000016751 RLG00000016752
rosa_multiflora Rmu_sc0002648.1_g000005 Rmu_sc0002648.1_g000006 Rmu_sc0004649.1_g000019 Rmu_sc0004649.1_g000021 Rmu_sc0009572.1_g000002 Rmu_sc0013919.1_g000008 Rmu_sc0013919.1_g000009
rosa_roxburghii Rroxscaffold_2G00144370 Rroxscaffold_2G00144380 Rroxscaffold_2G00144390 Rroxscaffold_2G00144410 Rroxscaffold_4G00294600 Rroxscaffold_7G00188840 Rroxscaffold_7G00188850 Rroxscaffold_7G00188860
rosa_rugosa Rorug02G0069400 Rorug02G0069600 Rorug02G0069700 Rorug02G0069800 Rorug06G0098000.1 Rorug06G0125000 Rorug06G0125100 Rorug06G0125200
rosa_samantha Rh2AG116400 Rh2AG116800 Rh2AG116900 Rh2AG117000 Rh2BG119300 Rh2BG119500 Rh2BG119600 Rh2BG119700 Rh2BG119800 Rh2CG120900 Rh2CG121000 Rh2CG121200 Rh2CG121300 Rh2CG121400 Rh2CG121500 Rh2DG120600 Rh2DG121100 Rh2DG121200 Rh2DG121500 Rh6AG091200 Rh6AG234100 Rh6BG238400 Rh6BG238500 Rh6BG238600 Rh6CG078800 Rh6CG240400 Rh6CG240500 Rh6CG240600 Rh6DG074600 Rh6DG232000 Rh6DG232100
rosa_wichuraiana Rw2G009120 Rw2G009130 Rw2G009140 Rw6G020420 Rw6G020430 Rw6G020440

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 3 cut(s) 12, 287, 824
AclI AACGTT 1 cut(s) 914
AclWI GGATC 2 cut(s) 70, 685
AcoI YGGCCR 2 cut(s) 3, 83
AcsI RAATTY 3 cut(s) 381, 465, 650
AfiI CCNNNNNNNGG 2 cut(s) 185, 350
AgsI TTSAA 2 cut(s) 450, 811
AjiI CACGTC 1 cut(s) 70
AluBI AGCT 2 cut(s) 637, 896
AluI AGCT 2 cut(s) 637, 896
Alw26I GTCTC 4 cut(s) 76, 516, 605, 862
AlwI GGATC 2 cut(s) 70, 685
AlwNI CAGNNNCTG 1 cut(s) 265
Ama87I CYCGRG 1 cut(s) 863
AoxI GGCC 6 cut(s) 3, 83, 168, 729, 772, 1083
ApeKI GCWGC 2 cut(s) 459, 1022
ApoI RAATTY 3 cut(s) 381, 465, 650
AspS9I GGNCC 5 cut(s) 168, 436, 482, 985, 1084
AsuHPI GGTGA 2 cut(s) 125, 427
AsuII TTCGAA 1 cut(s) 549
AvaI CYCGRG 1 cut(s) 863
AvaII GGWCC 3 cut(s) 436, 482, 985
BalI TGGCCA 1 cut(s) 5
BbvI GCAGC 2 cut(s) 471, 1034
BccI CCATC 3 cut(s) 282, 500, 1002
BceAI ACGGC 3 cut(s) 202, 916, 1078
BcoDI GTCTC 4 cut(s) 76, 516, 605, 862
BfaI CTAG 3 cut(s) 159, 254, 638
BfmI CTRYAG 3 cut(s) 120, 393, 1027
BisI GCNGC 2 cut(s) 460, 1023
BlsI GCNGC 2 cut(s) 461, 1024
Bme18I GGWCC 3 cut(s) 436, 482, 985
BmeT110I CYCGRG 1 cut(s) 863
BmgBI CACGTC 1 cut(s) 70
BmgT120I GGNCC 5 cut(s) 168, 436, 482, 985, 1084
BmiI GGNNCC 6 cut(s) 136, 483, 643, 986, 1045, 1062
BmrI ACTGGG 2 cut(s) 488, 582
BmsI GCATC 4 cut(s) 198, 550, 733, 817
BmuI ACTGGG 2 cut(s) 488, 582
Bpu14I TTCGAA 1 cut(s) 549
Bsa29I ATCGAT 2 cut(s) 192, 246
BsaAI YACGTR 1 cut(s) 1057
BsaI GGTCTC 1 cut(s) 862
BsaJI CCNNGG 2 cut(s) 769, 1039
BsaXI ACNNNNNCTCC 4 cut(s) 420, 450, 1028, 1058
Bsc4I CCNNNNNNNGG 2 cut(s) 185, 350
Bse1I ACTGG 4 cut(s) 86, 220, 483, 577
Bse3DI GCAATG 1 cut(s) 544
BseCI ATCGAT 2 cut(s) 192, 246
BseDI CCNNGG 2 cut(s) 769, 1039
BseGI GGATG 4 cut(s) 585, 748, 832, 994
BseLI CCNNNNNNNGG 2 cut(s) 185, 350
BseMI GCAATG 1 cut(s) 544
BseMII CTCAG 2 cut(s) 761, 786
BseNI ACTGG 4 cut(s) 86, 220, 483, 577
BseXI GCAGC 2 cut(s) 471, 1034
BseYI CCCAGC 2 cut(s) 258, 1002
BsgI GTGCAG 2 cut(s) 251, 747
BshFI GGCC 6 cut(s) 5, 85, 170, 731, 774, 1085
BshVI ATCGAT 2 cut(s) 192, 246
BsiHKCI CYCGRG 1 cut(s) 863
BslFI GGGAC 2 cut(s) 731, 998
BslI CCNNNNNNNGG 2 cut(s) 185, 350
BsmAI GTCTC 4 cut(s) 76, 516, 605, 862
BsmBI CGTCTC 1 cut(s) 76
BsmFI GGGAC 2 cut(s) 731, 998
BsmI GAATGC 1 cut(s) 493
BsnI GGCC 6 cut(s) 5, 85, 170, 731, 774, 1085
Bso31I GGTCTC 1 cut(s) 862
BsoBI CYCGRG 1 cut(s) 863
Bsp119I TTCGAA 1 cut(s) 549
Bsp143I GATC 4 cut(s) 62, 247, 690, 961
Bsp19I CCATGG 1 cut(s) 1039
BspACI CCGC 3 cut(s) 12, 287, 824
BspANI GGCC 6 cut(s) 5, 85, 170, 731, 774, 1085
BspCNI CTCAG 2 cut(s) 760, 787
BspDI ATCGAT 2 cut(s) 192, 246
BspLI GGNNCC 6 cut(s) 136, 483, 643, 986, 1045, 1062
BspPI GGATC 2 cut(s) 70, 685
BspT104I TTCGAA 1 cut(s) 549
BspTNI GGTCTC 1 cut(s) 862
BsrDI GCAATG 1 cut(s) 544
BsrI ACTGG 4 cut(s) 86, 220, 483, 577
BssECI CCNNGG 2 cut(s) 769, 1039
BssMI GATC 4 cut(s) 62, 247, 690, 961
BssT1I CCWWGG 1 cut(s) 1039
Bst4CI ACNGT 2 cut(s) 233, 722
BstBAI YACGTR 1 cut(s) 1057
BstBI TTCGAA 1 cut(s) 549
BstC8I GCNNGC 3 cut(s) 96, 898, 1008
BstDEI CTNAG 4 cut(s) 348, 620, 747, 795
BstDSI CCRYGG 1 cut(s) 1039
BstF5I GGATG 4 cut(s) 585, 748, 832, 994
BstKTI GATC 4 cut(s) 65, 250, 693, 964
BstMAI GTCTC 4 cut(s) 76, 516, 605, 862
BstMBI GATC 4 cut(s) 62, 247, 690, 961
BstMWI GCNNNNNNNGC 2 cut(s) 11, 91
BstSFI CTRYAG 3 cut(s) 120, 393, 1027
BstV1I GCAGC 2 cut(s) 471, 1034
BstXI CCANNNNNNTGG 1 cut(s) 739
Bsu15I ATCGAT 2 cut(s) 192, 246
BsuRI GGCC 6 cut(s) 5, 85, 170, 731, 774, 1085
BsuTUI ATCGAT 2 cut(s) 192, 246
BtgI CCRYGG 1 cut(s) 1039
BtgZI GCGATG 1 cut(s) 478
BtrI CACGTC 1 cut(s) 70
BtsCI GGATG 4 cut(s) 585, 748, 832, 994
BtsIMutI CAGTG 2 cut(s) 532, 570
Cac8I GCNNGC 3 cut(s) 96, 898, 1008
CaiI CAGNNNCTG 1 cut(s) 265
Cfr13I GGNCC 5 cut(s) 168, 436, 482, 985, 1084
ClaI ATCGAT 2 cut(s) 192, 246
CviAII CATG 2 cut(s) 733, 1040
DdeI CTNAG 4 cut(s) 348, 620, 747, 795
DpnI GATC 4 cut(s) 64, 249, 692, 963
DpnII GATC 4 cut(s) 62, 247, 690, 961
DraI TTTAAA 1 cut(s) 880
EaeI YGGCCR 2 cut(s) 3, 83
Eco130I CCWWGG 1 cut(s) 1039
Eco147I AGGCCT 1 cut(s) 774
Eco31I GGTCTC 1 cut(s) 862
Eco47I GGWCC 3 cut(s) 436, 482, 985
Eco88I CYCGRG 1 cut(s) 863
EcoRI GAATTC 1 cut(s) 381
EcoT14I CCWWGG 1 cut(s) 1039
EcoT22I ATGCAT 1 cut(s) 832
ErhI CCWWGG 1 cut(s) 1039
Esp3I CGTCTC 1 cut(s) 76
FaeI CATG 2 cut(s) 736, 1043
FalI AAGNNNNNCTT 2 cut(s) 500, 532
FaqI GGGAC 2 cut(s) 731, 998
FatI CATG 2 cut(s) 732, 1039
FauI CCCGC 1 cut(s) 294
Fnu4HI GCNGC 2 cut(s) 460, 1023
FokI GGATG 4 cut(s) 572, 755, 839, 981
Fsp4HI GCNGC 2 cut(s) 460, 1023
FspBI CTAG 3 cut(s) 159, 254, 638
GluI GCNGC 2 cut(s) 460, 1023
GsaI CCCAGC 2 cut(s) 262, 1006
HaeIII GGCC 6 cut(s) 5, 85, 170, 731, 774, 1085
Hin1II CATG 2 cut(s) 736, 1043
HinfI GANTC 4 cut(s) 116, 354, 613, 944
HphI GGTGA 2 cut(s) 125, 427
Hpy188I TCNGA 6 cut(s) 53, 472, 486, 690, 770, 796
Hpy188III TCNNGA 4 cut(s) 159, 305, 369, 378
HpyAV CCTTC 1 cut(s) 1097
HpyCH4III ACNGT 2 cut(s) 233, 722
HpyCH4IV ACGT 4 cut(s) 69, 697, 914, 1056
HpyCH4V TGCA 7 cut(s) 268, 459, 764, 781, 801, 818, 830
HpyF10VI GCNNNNNNNGC 2 cut(s) 11, 91
HpyF3I CTNAG 4 cut(s) 348, 620, 747, 795
HpySE526I ACGT 4 cut(s) 69, 697, 914, 1056
Hsp92II CATG 2 cut(s) 736, 1043
Kzo9I GATC 4 cut(s) 62, 247, 690, 961
LmnI GCTCC 3 cut(s) 641, 1049, 1060
Lsp1109I GCAGC 2 cut(s) 471, 1034
LweI GCATC 4 cut(s) 198, 550, 733, 817
MaeI CTAG 3 cut(s) 159, 254, 638
MaeII ACGT 4 cut(s) 69, 697, 914, 1056
MaeIII GTNAC 1 cut(s) 233
MalI GATC 4 cut(s) 64, 249, 692, 963
MboI GATC 4 cut(s) 62, 247, 690, 961
MboII GAAGA 3 cut(s) 231, 242, 538
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 7 cut(s) 154, 323, 381, 443, 465, 650, 660
MluNI TGGCCA 1 cut(s) 5
MlyI GAGTC 1 cut(s) 607
MmeI TCCRAC 6 cut(s) 112, 368, 463, 511, 718, 1022
MnlI CCTC 6 cut(s) 159, 210, 365, 721, 764, 785
Mox20I TGGCCA 1 cut(s) 5
Mph1103I ATGCAT 1 cut(s) 832
MscI TGGCCA 1 cut(s) 5
MseI TTAA 4 cut(s) 33, 183, 879, 917
MslI CAYNNNNRTG 2 cut(s) 558, 737
Msp20I TGGCCA 1 cut(s) 5
Mva1269I GAATGC 1 cut(s) 493
MwoI GCNNNNNNNGC 2 cut(s) 11, 91
NcoI CCATGG 1 cut(s) 1039
NdeII GATC 4 cut(s) 62, 247, 690, 961
NlaIII CATG 2 cut(s) 736, 1043
NlaIV GGNNCC 6 cut(s) 136, 483, 643, 986, 1045, 1062
NmeAIII GCCGAG 1 cut(s) 61
NsiI ATGCAT 1 cut(s) 832
NspV TTCGAA 1 cut(s) 549
PceI AGGCCT 1 cut(s) 774
PcsI WCGNNNNNNNCGW 1 cut(s) 574
PctI GAATGC 1 cut(s) 493
PfeI GAWTC 3 cut(s) 116, 354, 944
PkrI GCNGC 2 cut(s) 461, 1024
PleI GAGTC 1 cut(s) 607
PpsI GAGTC 1 cut(s) 607
Ppu21I YACGTR 1 cut(s) 1057
Psp1406I AACGTT 1 cut(s) 914
PspFI CCCAGC 2 cut(s) 258, 1002
PspN4I GGNNCC 6 cut(s) 136, 483, 643, 986, 1045, 1062
PspPI GGNCC 5 cut(s) 168, 436, 482, 985, 1084
PstNI CAGNNNCTG 1 cut(s) 265
RseI CAYNNNNRTG 2 cut(s) 558, 737
SaqAI TTAA 4 cut(s) 33, 183, 879, 917
SatI GCNGC 2 cut(s) 460, 1023
Sau3AI GATC 4 cut(s) 62, 247, 690, 961
Sau96I GGNCC 5 cut(s) 168, 436, 482, 985, 1084
SchI GAGTC 1 cut(s) 607
SfaNI GCATC 4 cut(s) 198, 550, 733, 817
SfcI CTRYAG 3 cut(s) 120, 393, 1027
SfuI TTCGAA 1 cut(s) 549
SinI GGWCC 3 cut(s) 436, 482, 985
SmiMI CAYNNNNRTG 2 cut(s) 558, 737
Sse9I AATT 7 cut(s) 154, 323, 381, 443, 465, 650, 660
SseBI AGGCCT 1 cut(s) 774
SsiI CCGC 3 cut(s) 12, 287, 824
SspI AATATT 1 cut(s) 18
SspMI CTAG 3 cut(s) 159, 254, 638
StuI AGGCCT 1 cut(s) 774
StyI CCWWGG 1 cut(s) 1039
TaaI ACNGT 2 cut(s) 233, 722
TaiI ACGT 4 cut(s) 72, 700, 917, 1059
TaqI TCGA 4 cut(s) 192, 246, 549, 704
TasI AATT 7 cut(s) 154, 323, 381, 443, 465, 650, 660
TfiI GAWTC 3 cut(s) 116, 354, 944
Tru1I TTAA 4 cut(s) 33, 183, 879, 917
Tru9I TTAA 4 cut(s) 33, 183, 879, 917
TscAI CASTG 2 cut(s) 532, 577
TseI GCWGC 2 cut(s) 459, 1022
TspDTI ATGAA 2 cut(s) 749, 902
TspGWI ACGGA 1 cut(s) 924
TspRI CASTG 2 cut(s) 532, 577
VpaK11BI GGWCC 3 cut(s) 436, 482, 985
XapI RAATTY 3 cut(s) 381, 465, 650
XbaI TCTAGA 1 cut(s) 158
XspI CTAG 3 cut(s) 159, 254, 638
Zsp2I ATGCAT 1 cut(s) 832
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.