FvH4_2g15980

GDSL esterase lipase At5g55050-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0000448
Physical Location & Seq
Reverse (-)
13967785 .. 13969433
1649 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g15980.t1

Sequence Viewer

Length: 1143 bp
ATGGAGAACAAATGGATTCTTCCTCTAGCCCTATCCTTGTTTATAGCTGTTCTTAGCTTGGCAGCAGCAGCTAAGCCAGTACTGCCACCAGTCTTCATACTTGGTGATTCAACTGCAGATGTTGGTACTAATAATTTCTTGCCGGGCAGTATGGCAAGAGCTGACTTTCCCCACAATGGCATTGACTTTCCTCGCACTCCGAAACCCACCGGAAGGTTCAGCAATGGCCTTAACAGTGCTGATTTTCTTGCCCAGCATTTTGGTTACAAGAGAAGTCCAAGCCCCTTTCTTTCTTTCAAAAGTACTTCTCTTCAAAAGAAGAAGTTTAATGGTATCAACTTTGCTTCTGGAGGGTCTGGTCTTCTTGACCTAACTGGACAGACAATGCTGACGTTGATGAAGTTTGGAACTGGAAATGTTCCATTTAGTGCCTCATTCAAAGACCAGAAGAATGTGATCTCATTAACAGAACAGATACAACAATTTTCATCTGTCAAAAACAATCTTACGGCCTTGATGGGTGCAGTGGCAACTGAGAAGTTTCTTAAAGAGTCTTTGATCTTCATCAGCATCGGCAGCAATGACCTTTTCGCATACTACCATTCAAAGAGTTCCATTCCGAAGGAAAAGTTCTTGTGCTCTTTAGAACTCGCTTATGAGAACCACTTGAAGACTCTATACAATCTTGGAGCAAGGAAGTTTGGCATCATCAGCGTTGCTCCAATTGGGTGCTGTCCATCTCAGAGGATTTTCAATGCTACTGGGGGATGTTTGGAGGAGTTGAATGAGTATGCAGTAACCTTCCACTCGAAGCTGGACGCCCTCTTGTGCAAGCTCAGCACCAAATACGAAGGCTTTAAGTATTCCCTTGGAAATTCATATGAAATGACAATGAATGTCATACAGAACCCTCTTCCATTCAATTTTACACAAGTGGAAGCTGCATGTTGTGGAGCTGGGAAGCTCAATGCTGAATCCTACTGCACACCAAATGCTAATCTTTGTCCAAACCGTGACCAATACTTGTTCTGGGATCTATTTCATCCATCACAGGCGGCTTCGAAGTTGGCAGCTGTGACCCTCTACTTTGGAGGACCAGAATATGTATCCCCAATTAACTTCGCTCAGTTAGCCGAGGCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

381

Amino Acids

41.5

Weight (kDa)

8.6

Isoelectric Point (pI)

26.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 31 - 358 1.8e-28 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000448)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g10790 FvH4_1g10790 FvH4_1g10790 FvH4_1g10791 FvH4_1g10800 FvH4_2g15970 FvH4_2g15980 FvH4_2g15990
malus_domestica MD02G1121900.v1.1 MD05G1128700.v1.1 MD10G1131900.v1.1 MD15G1235500.v1.1 MD15G1235600.v1.1
prunus_persica Prupe.7G177500_v2.0.a1 Prupe.7G177600_v2.0.a1 Prupe.8G172800_v2.0.a1
pyrus_communis pycom05g12460 pycom05g12470 pycom10g11300
rosa_chinensis RchiOBHm_Chr2g0097971 RchiOBHm_Chr2g0098021 RchiOBHm_Chr2g0098061 RchiOBHm_Chr6g0257301 RchiOBHm_Chr6g0279511 RchiOBHm_Chr6g0279521
rosa_laevigata RLG00000013139 RLG00000013140 RLG00000013141 RLG00000016747 RLG00000016749 RLG00000016750 RLG00000016751 RLG00000016752
rosa_multiflora Rmu_sc0002648.1_g000005 Rmu_sc0002648.1_g000006 Rmu_sc0004649.1_g000019 Rmu_sc0004649.1_g000021 Rmu_sc0009572.1_g000002 Rmu_sc0013919.1_g000008 Rmu_sc0013919.1_g000009
rosa_roxburghii Rroxscaffold_2G00144370 Rroxscaffold_2G00144380 Rroxscaffold_2G00144390 Rroxscaffold_2G00144410 Rroxscaffold_4G00294600 Rroxscaffold_7G00188840 Rroxscaffold_7G00188850 Rroxscaffold_7G00188860
rosa_rugosa Rorug02G0069400 Rorug02G0069600 Rorug02G0069700 Rorug02G0069800 Rorug06G0098000.1 Rorug06G0125000 Rorug06G0125100 Rorug06G0125200
rosa_samantha Rh2AG116400 Rh2AG116800 Rh2AG116900 Rh2AG117000 Rh2BG119300 Rh2BG119500 Rh2BG119600 Rh2BG119700 Rh2BG119800 Rh2CG120900 Rh2CG121000 Rh2CG121200 Rh2CG121300 Rh2CG121400 Rh2CG121500 Rh2DG120600 Rh2DG121100 Rh2DG121200 Rh2DG121500 Rh6AG091200 Rh6AG234100 Rh6BG238400 Rh6BG238500 Rh6BG238600 Rh6CG078800 Rh6CG240400 Rh6CG240500 Rh6CG240600 Rh6DG074600 Rh6DG232000 Rh6DG232100
rosa_wichuraiana Rw2G009120 Rw2G009130 Rw2G009140 Rw6G020420 Rw6G020430 Rw6G020440

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 1055
AclWI GGATC 1 cut(s) 1041
AcsI RAATTY 1 cut(s) 874
AcyI GRCGYC 1 cut(s) 819
AfaI GTAC 3 cut(s) 81, 127, 304
AfiI CCNNNNNNNGG 2 cut(s) 176, 213
AgsI TTSAA 9 cut(s) 111, 298, 314, 439, 606, 670, 754, 784, 922
Alw21I GWGCWC 1 cut(s) 641
AlwI GGATC 1 cut(s) 1041
AoxI GGCC 3 cut(s) 226, 510, 1137
ApeKI GCWGC 6 cut(s) 62, 65, 68, 576, 941, 1070
ApoI RAATTY 1 cut(s) 874
AspS9I GGNCC 1 cut(s) 1094
AsuC2I CCSGG 1 cut(s) 144
AsuHPI GGTGA 1 cut(s) 116
AsuII TTCGAA 1 cut(s) 1061
AvaII GGWCC 1 cut(s) 1094
BarI GAAGNNNNNNTAC 2 cut(s) 662, 694
BbsI GAAGAC 3 cut(s) 85, 353, 677
Bbv12I GWGCWC 1 cut(s) 641
BbvI GCAGC 6 cut(s) 74, 77, 80, 588, 928, 1082
BccI CCATC 3 cut(s) 511, 745, 1054
BceAI ACGGC 1 cut(s) 525
BciVI GTATCC 1 cut(s) 1117
BcnI CCSGG 1 cut(s) 144
BfaI CTAG 2 cut(s) 26, 1141
BfmI CTRYAG 1 cut(s) 114
BfuI GTATCC 1 cut(s) 1117
BisI GCNGC 7 cut(s) 63, 66, 69, 577, 942, 1056, 1071
BlpI GCTNAGC 2 cut(s) 72, 836
BlsI GCNGC 7 cut(s) 64, 67, 70, 578, 943, 1057, 1072
BmcAI AGTACT 2 cut(s) 81, 304
Bme1390I CCNGG 1 cut(s) 144
Bme18I GGWCC 1 cut(s) 1094
BmgT120I GGNCC 1 cut(s) 1094
BmrFI CCNGG 1 cut(s) 144
BmrI ACTGGG 1 cut(s) 771
BmsI GCATC 2 cut(s) 579, 714
BmuI ACTGGG 1 cut(s) 771
BpiI GAAGAC 3 cut(s) 85, 353, 677
BpmI CTGGAG 1 cut(s) 369
Bpu1102I GCTNAGC 2 cut(s) 72, 836
Bpu14I TTCGAA 1 cut(s) 1061
BpuMI CCSGG 1 cut(s) 144
BsaBI GATNNNNATC 1 cut(s) 563
BsaHI GRCGYC 1 cut(s) 819
BsaJI CCNNGG 2 cut(s) 868, 1134
BsaWI WCCGGW 1 cut(s) 209
Bsc4I CCNNNNNNNGG 2 cut(s) 176, 213
Bse1I ACTGG 5 cut(s) 77, 89, 379, 415, 766
Bse3DI GCAATG 2 cut(s) 229, 586
Bse8I GATNNNNATC 1 cut(s) 563
BseDI CCNNGG 2 cut(s) 868, 1134
BseGI GGATG 2 cut(s) 773, 1042
BseJI GATNNNNATC 1 cut(s) 563
BseLI CCNNNNNNNGG 2 cut(s) 176, 213
BseMI GCAATG 2 cut(s) 229, 586
BseMII CTCAG 4 cut(s) 525, 755, 850, 1139
BseNI ACTGG 5 cut(s) 77, 89, 379, 415, 766
BseRI GAGGAG 1 cut(s) 791
BseXI GCAGC 6 cut(s) 74, 77, 80, 588, 928, 1082
BseYI CCCAGC 2 cut(s) 252, 956
BsgI GTGCAG 2 cut(s) 543, 967
BshFI GGCC 3 cut(s) 228, 512, 1139
BsiHKAI GWGCWC 1 cut(s) 641
BsiSI CCGG 2 cut(s) 143, 210
BslI CCNNNNNNNGG 2 cut(s) 176, 213
BsnI GGCC 3 cut(s) 228, 512, 1139
Bsp119I TTCGAA 1 cut(s) 1061
Bsp1286I GDGCHC 1 cut(s) 641
Bsp143I GATC 3 cut(s) 456, 558, 1033
Bsp1720I GCTNAGC 2 cut(s) 72, 836
BspACI CCGC 1 cut(s) 1055
BspANI GGCC 3 cut(s) 228, 512, 1139
BspCNI CTCAG 4 cut(s) 526, 754, 849, 1138
BspMAI CTGCAG 1 cut(s) 118
BspPI GGATC 1 cut(s) 1041
BspT104I TTCGAA 1 cut(s) 1061
BsrDI GCAATG 2 cut(s) 229, 586
BsrI ACTGG 5 cut(s) 77, 89, 379, 415, 766
BssECI CCNNGG 2 cut(s) 868, 1134
BssMI GATC 3 cut(s) 456, 558, 1033
BssNI GRCGYC 1 cut(s) 819
BssT1I CCWWGG 1 cut(s) 868
Bst4CI ACNGT 2 cut(s) 236, 1013
Bst6I CTCTTC 2 cut(s) 315, 918
BstACI GRCGYC 1 cut(s) 819
BstBI TTCGAA 1 cut(s) 1061
BstC8I GCNNGC 1 cut(s) 833
BstDEI CTNAG 6 cut(s) 53, 72, 534, 741, 836, 1125
BstF5I GGATG 2 cut(s) 773, 1042
BstKTI GATC 3 cut(s) 459, 561, 1036
BstMBI GATC 3 cut(s) 456, 558, 1033
BstMWI GCNNNNNNNGC 6 cut(s) 68, 82, 576, 711, 837, 1130
BstNSI RCATGY 1 cut(s) 948
BstSCI CCNGG 1 cut(s) 142
BstSFI CTRYAG 1 cut(s) 114
BstV1I GCAGC 6 cut(s) 74, 77, 80, 588, 928, 1082
BstV2I GAAGAC 3 cut(s) 85, 353, 677
BstX2I RGATCY 1 cut(s) 1033
BstXI CCANNNNNNTGG 1 cut(s) 260
BstYI RGATCY 1 cut(s) 1033
BsuI GTATCC 1 cut(s) 1117
BsuRI GGCC 3 cut(s) 228, 512, 1139
BtsCI GGATG 2 cut(s) 773, 1042
BtsI GCAGTG 1 cut(s) 531
BtsIMutI CAGTG 2 cut(s) 241, 531
Cac8I GCNNGC 1 cut(s) 833
Cfr13I GGNCC 1 cut(s) 1094
CseI GACGC 1 cut(s) 827
Csp6I GTAC 3 cut(s) 80, 126, 303
CviAII CATG 1 cut(s) 945
CviQI GTAC 3 cut(s) 80, 126, 303
DdeI CTNAG 6 cut(s) 53, 72, 534, 741, 836, 1125
DpnI GATC 3 cut(s) 458, 560, 1035
DpnII GATC 3 cut(s) 456, 558, 1033
Eam1104I CTCTTC 2 cut(s) 315, 918
EarI CTCTTC 2 cut(s) 315, 918
Eco130I CCWWGG 1 cut(s) 868
Eco147I AGGCCT 1 cut(s) 1139
Eco47I GGWCC 1 cut(s) 1094
EcoT14I CCWWGG 1 cut(s) 868
ErhI CCWWGG 1 cut(s) 868
FaeI CATG 1 cut(s) 948
FatI CATG 1 cut(s) 944
FauNDI CATATG 1 cut(s) 880
Fnu4HI GCNGC 7 cut(s) 63, 66, 69, 577, 942, 1056, 1071
FokI GGATG 2 cut(s) 780, 1029
Fsp4HI GCNGC 7 cut(s) 63, 66, 69, 577, 942, 1056, 1071
FspBI CTAG 2 cut(s) 26, 1141
GluI GCNGC 7 cut(s) 63, 66, 69, 577, 942, 1056, 1071
GsaI CCCAGC 2 cut(s) 256, 960
GsuI CTGGAG 1 cut(s) 369
HaeIII GGCC 3 cut(s) 228, 512, 1139
HapII CCGG 2 cut(s) 143, 210
HgaI GACGC 1 cut(s) 827
Hin1I GRCGYC 1 cut(s) 819
Hin1II CATG 1 cut(s) 948
HinfI GANTC 5 cut(s) 16, 107, 551, 673, 974
HpaII CCGG 2 cut(s) 143, 210
HphI GGTGA 1 cut(s) 116
Hpy188I TCNGA 3 cut(s) 201, 621, 744
Hpy188III TCNNGA 2 cut(s) 348, 365
HpyAV CCTTC 4 cut(s) 207, 616, 811, 845
HpyCH4III ACNGT 2 cut(s) 236, 1013
HpyCH4IV ACGT 1 cut(s) 392
HpyCH4V TGCA 6 cut(s) 116, 524, 794, 831, 944, 984
HpyF10VI GCNNNNNNNGC 6 cut(s) 68, 82, 576, 711, 837, 1130
HpyF3I CTNAG 6 cut(s) 53, 72, 534, 741, 836, 1125
HpySE526I ACGT 1 cut(s) 392
Hsp92I GRCGYC 1 cut(s) 819
Hsp92II CATG 1 cut(s) 948
Kzo9I GATC 3 cut(s) 456, 558, 1033
LmnI GCTCC 3 cut(s) 689, 724, 953
Lsp1109I GCAGC 6 cut(s) 74, 77, 80, 588, 928, 1082
LweI GCATC 2 cut(s) 579, 714
MaeI CTAG 2 cut(s) 26, 1141
MaeII ACGT 1 cut(s) 392
MaeIII GTNAC 4 cut(s) 263, 796, 1013, 1075
MalI GATC 3 cut(s) 458, 560, 1035
MboI GATC 3 cut(s) 456, 558, 1033
MboII GAAGA 9 cut(s) 11, 85, 302, 331, 353, 460, 553, 682, 905
MfeI CAATTG 1 cut(s) 723
MflI RGATCY 1 cut(s) 1033
MhlI GDGCHC 1 cut(s) 641
MluCI AATT 6 cut(s) 133, 482, 723, 874, 922, 1113
MlyI GAGTC 2 cut(s) 560, 667
MseI TTAA 6 cut(s) 231, 327, 464, 546, 858, 1116
MspA1I CMGCKG 1 cut(s) 1073
MspI CCGG 2 cut(s) 143, 210
MspR9I CCNGG 1 cut(s) 144
MunI CAATTG 1 cut(s) 723
MwoI GCNNNNNNNGC 6 cut(s) 68, 82, 576, 711, 837, 1130
NciI CCSGG 1 cut(s) 144
NdeI CATATG 1 cut(s) 880
NdeII GATC 3 cut(s) 456, 558, 1033
NlaIII CATG 1 cut(s) 948
NmuCI GTSAC 2 cut(s) 1013, 1075
NspI RCATGY 1 cut(s) 948
NspV TTCGAA 1 cut(s) 1061
PceI AGGCCT 1 cut(s) 1139
PfeI GAWTC 3 cut(s) 16, 107, 974
PkrI GCNGC 7 cut(s) 64, 67, 70, 578, 943, 1057, 1072
PleI GAGTC 2 cut(s) 559, 667
PpsI GAGTC 2 cut(s) 559, 667
PspFI CCCAGC 2 cut(s) 252, 956
PspPI GGNCC 1 cut(s) 1094
PstI CTGCAG 1 cut(s) 118
PsuI RGATCY 1 cut(s) 1033
PvuII CAGCTG 1 cut(s) 1073
RsaI GTAC 3 cut(s) 81, 127, 304
RsaNI GTAC 3 cut(s) 80, 126, 303
SaqAI TTAA 6 cut(s) 231, 327, 464, 546, 858, 1116
SatI GCNGC 7 cut(s) 63, 66, 69, 577, 942, 1056, 1071
Sau3AI GATC 3 cut(s) 456, 558, 1033
Sau96I GGNCC 1 cut(s) 1094
ScaI AGTACT 2 cut(s) 81, 304
SchI GAGTC 2 cut(s) 560, 667
ScrFI CCNGG 1 cut(s) 144
SduI GDGCHC 1 cut(s) 641
SfaNI GCATC 2 cut(s) 579, 714
SfcI CTRYAG 1 cut(s) 114
SfuI TTCGAA 1 cut(s) 1061
SinI GGWCC 1 cut(s) 1094
Sse9I AATT 6 cut(s) 133, 482, 723, 874, 922, 1113
SseBI AGGCCT 1 cut(s) 1139
SsiI CCGC 1 cut(s) 1055
SspMI CTAG 2 cut(s) 26, 1141
StuI AGGCCT 1 cut(s) 1139
StyD4I CCNGG 1 cut(s) 142
StyI CCWWGG 1 cut(s) 868
TaaI ACNGT 2 cut(s) 236, 1013
TaiI ACGT 1 cut(s) 395
TaqI TCGA 2 cut(s) 809, 1061
TasI AATT 6 cut(s) 133, 482, 723, 874, 922, 1113
TatI WGTACW 2 cut(s) 79, 302
TauI GCSGC 1 cut(s) 1058
TfiI GAWTC 3 cut(s) 16, 107, 974
Tru1I TTAA 6 cut(s) 231, 327, 464, 546, 858, 1116
Tru9I TTAA 6 cut(s) 231, 327, 464, 546, 858, 1116
TscAI CASTG 2 cut(s) 241, 531
TseFI GTSAC 2 cut(s) 1013, 1075
TseI GCWGC 6 cut(s) 62, 65, 68, 576, 941, 1070
Tsp45I GTSAC 2 cut(s) 1013, 1075
TspDTI ATGAA 8 cut(s) 85, 413, 477, 553, 867, 897, 908, 1031
TspRI CASTG 2 cut(s) 241, 531
VpaK11BI GGWCC 1 cut(s) 1094
XapI RAATTY 1 cut(s) 874
XceI RCATGY 1 cut(s) 948
XspI CTAG 2 cut(s) 26, 1141
ZrmI AGTACT 2 cut(s) 81, 304
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.