Rw2G009130

GDSL esterase lipase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0000448
Physical Location & Seq
Forward (+)
9187770 .. 9191025
3256 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G009130.1

Sequence Viewer

Length: 579 bp
ATGGCCAATAACAATGGGTGGACACCCTTGTTTTCTGTTTGGTTTTTTGTGTTTGTATATCTGGCCATGCTAGGTTTCCATGTATCCTCTGCACAGAAAATTGTGCCTGCAATTTACATATTTGGAGATTCTTCAGGAGATGTTGGAACCAATAATTACTTGCCTGATTGTACCGCAAGGGCTGATATCCTCTATAATGGGATTGACTACTCTCACTCTAAACCAACAGGAAGATTTAGCAACGTACGGTTACAACCCCAGTTTCTGGGTTACAAGAAGAGCCCACCACCTTTTCTCTCTCTTTTAGACGAGAAGAAGCAATTTCCTAACAGGAAGGGAAATGTCATTTCCTTGGGGGAACAAGTGCAGCAATTCCAAACTTTACGGAACAAAATCTCAGAATTACTGGGTGCTGGTGGATTGGCTAATATTTCTCAGTCTCTCTCTGTCATCAACGTTGGAAGCAACGACATATTTGAATTATTTGCTGCCAATAACCTGACTAAAAGAACCGAGCAAGACTACTTGAGCATACTCATCTCCTCTTACGAAACTCATTCAAGGGTTGGACACTGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

192

Amino Acids

21.36

Weight (kDa)

8.52

Isoelectric Point (pI)

38.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 38 - 183 4.6e-06 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000448)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g10790 FvH4_1g10790 FvH4_1g10790 FvH4_1g10791 FvH4_1g10800 FvH4_2g15970 FvH4_2g15980 FvH4_2g15990
malus_domestica MD02G1121900.v1.1 MD05G1128700.v1.1 MD10G1131900.v1.1 MD15G1235500.v1.1 MD15G1235600.v1.1
prunus_persica Prupe.7G177500_v2.0.a1 Prupe.7G177600_v2.0.a1 Prupe.8G172800_v2.0.a1
pyrus_communis pycom05g12460 pycom05g12470 pycom10g11300
rosa_chinensis RchiOBHm_Chr2g0097971 RchiOBHm_Chr2g0098021 RchiOBHm_Chr2g0098061 RchiOBHm_Chr6g0257301 RchiOBHm_Chr6g0279511 RchiOBHm_Chr6g0279521
rosa_laevigata RLG00000013139 RLG00000013140 RLG00000013141 RLG00000016747 RLG00000016749 RLG00000016750 RLG00000016751 RLG00000016752
rosa_multiflora Rmu_sc0002648.1_g000005 Rmu_sc0002648.1_g000006 Rmu_sc0004649.1_g000019 Rmu_sc0004649.1_g000021 Rmu_sc0009572.1_g000002 Rmu_sc0013919.1_g000008 Rmu_sc0013919.1_g000009
rosa_roxburghii Rroxscaffold_2G00144370 Rroxscaffold_2G00144380 Rroxscaffold_2G00144390 Rroxscaffold_2G00144410 Rroxscaffold_4G00294600 Rroxscaffold_7G00188840 Rroxscaffold_7G00188850 Rroxscaffold_7G00188860
rosa_rugosa Rorug02G0069400 Rorug02G0069600 Rorug02G0069700 Rorug02G0069800 Rorug06G0098000.1 Rorug06G0125000 Rorug06G0125100 Rorug06G0125200
rosa_samantha Rh2AG116400 Rh2AG116800 Rh2AG116900 Rh2AG117000 Rh2BG119300 Rh2BG119500 Rh2BG119600 Rh2BG119700 Rh2BG119800 Rh2CG120900 Rh2CG121000 Rh2CG121200 Rh2CG121300 Rh2CG121400 Rh2CG121500 Rh2DG120600 Rh2DG121100 Rh2DG121200 Rh2DG121500 Rh6AG091200 Rh6AG234100 Rh6BG238400 Rh6BG238500 Rh6BG238600 Rh6CG078800 Rh6CG240400 Rh6CG240500 Rh6CG240600 Rh6DG074600 Rh6DG232000 Rh6DG232100
rosa_wichuraiana Rw2G009120 Rw2G009130 Rw2G009140 Rw6G020420 Rw6G020430 Rw6G020440

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 265
AciI CCGC 1 cut(s) 174
AclI AACGTT 1 cut(s) 456
AcoI YGGCCR 2 cut(s) 3, 63
AcuI CTGAAG 1 cut(s) 117
AfaI GTAC 2 cut(s) 172, 246
AfiI CCNNNNNNNGG 1 cut(s) 265
AgsI TTSAA 2 cut(s) 479, 561
Alw26I GTCTC 1 cut(s) 444
AlwNI CAGNNNCTG 1 cut(s) 265
AoxI GGCC 2 cut(s) 3, 63
ApeKI GCWGC 2 cut(s) 367, 488
BalI TGGCCA 2 cut(s) 5, 65
BanII GRGCYC 1 cut(s) 284
BbvI GCAGC 2 cut(s) 379, 475
BciVI GTATCC 1 cut(s) 94
BcoDI GTCTC 1 cut(s) 444
BfaI CTAG 2 cut(s) 71, 577
BfuI GTATCC 1 cut(s) 94
BisI GCNGC 2 cut(s) 368, 489
BlsI GCNGC 2 cut(s) 369, 490
BmiI GGNNCC 1 cut(s) 148
BmrI ACTGGG 2 cut(s) 253, 416
BmuI ACTGGG 2 cut(s) 253, 416
BpuEI CTTGAG 1 cut(s) 547
BsaJI CCNNGG 1 cut(s) 351
Bsc4I CCNNNNNNNGG 1 cut(s) 265
Bse1I ACTGG 2 cut(s) 259, 411
BseDI CCNNGG 1 cut(s) 351
BseLI CCNNNNNNNGG 1 cut(s) 265
BseMII CTCAG 2 cut(s) 411, 449
BseNI ACTGG 2 cut(s) 259, 411
BseRI GAGGAG 1 cut(s) 532
BseXI GCAGC 2 cut(s) 379, 475
BsgI GTGCAG 2 cut(s) 75, 386
BshFI GGCC 2 cut(s) 5, 65
BsiWI CGTACG 1 cut(s) 244
BslI CCNNNNNNNGG 1 cut(s) 265
BsmAI GTCTC 1 cut(s) 444
BsnI GGCC 2 cut(s) 5, 65
Bsp1286I GDGCHC 1 cut(s) 284
BspACI CCGC 1 cut(s) 174
BspANI GGCC 2 cut(s) 5, 65
BspCNI CTCAG 2 cut(s) 410, 448
BspLI GGNNCC 1 cut(s) 148
BspQI GCTCTTC 1 cut(s) 272
BsrI ACTGG 2 cut(s) 259, 411
BssECI CCNNGG 1 cut(s) 351
BssT1I CCWWGG 1 cut(s) 351
Bst4CI ACNGT 1 cut(s) 249
Bst6I CTCTTC 1 cut(s) 272
BstC8I GCNNGC 1 cut(s) 108
BstDEI CTNAG 2 cut(s) 397, 435
BstMAI GTCTC 1 cut(s) 444
BstV1I GCAGC 2 cut(s) 379, 475
BsuI GTATCC 1 cut(s) 94
BsuRI GGCC 2 cut(s) 5, 65
BtsI GCAGTG 1 cut(s) 571
BtsIMutI CAGTG 1 cut(s) 571
Cac8I GCNNGC 1 cut(s) 108
CaiI CAGNNNCTG 1 cut(s) 265
Csp6I GTAC 2 cut(s) 171, 245
CviAII CATG 2 cut(s) 67, 80
CviJI RGCY 5 cut(s) 5, 65, 182, 282, 425
CviKI_1 RGCY 5 cut(s) 5, 65, 182, 282, 425
CviQI GTAC 2 cut(s) 171, 245
DdeI CTNAG 2 cut(s) 397, 435
EaeI YGGCCR 2 cut(s) 3, 63
Eam1104I CTCTTC 1 cut(s) 272
EarI CTCTTC 1 cut(s) 272
Eco130I CCWWGG 1 cut(s) 351
Eco24I GRGCYC 1 cut(s) 284
Eco32I GATATC 1 cut(s) 187
Eco57I CTGAAG 1 cut(s) 117
EcoRV GATATC 1 cut(s) 187
EcoT14I CCWWGG 1 cut(s) 351
EcoT38I GRGCYC 1 cut(s) 284
ErhI CCWWGG 1 cut(s) 351
FaeI CATG 2 cut(s) 70, 83
FaiI YATR 7 cut(s) 58, 68, 81, 119, 195, 473, 533
FatI CATG 2 cut(s) 66, 79
Fnu4HI GCNGC 2 cut(s) 368, 489
FriOI GRGCYC 1 cut(s) 284
Fsp4HI GCNGC 2 cut(s) 368, 489
FspBI CTAG 2 cut(s) 71, 577
GluI GCNGC 2 cut(s) 368, 489
HaeIII GGCC 2 cut(s) 5, 65
Hin1II CATG 2 cut(s) 70, 83
HinfI GANTC 1 cut(s) 128
Hpy166II GTNNAC 1 cut(s) 21
Hpy188I TCNGA 1 cut(s) 400
Hpy188III TCNNGA 1 cut(s) 135
Hpy8I GTNNAC 1 cut(s) 21
HpyAV CCTTC 1 cut(s) 328
HpyCH4III ACNGT 1 cut(s) 249
HpyCH4IV ACGT 2 cut(s) 243, 456
HpyCH4V TGCA 3 cut(s) 92, 110, 367
HpyF3I CTNAG 2 cut(s) 397, 435
HpySE526I ACGT 2 cut(s) 243, 456
Hsp92II CATG 2 cut(s) 70, 83
LguI GCTCTTC 1 cut(s) 272
Lsp1109I GCAGC 2 cut(s) 379, 475
MaeI CTAG 2 cut(s) 71, 577
MaeII ACGT 2 cut(s) 243, 456
MaeIII GTNAC 2 cut(s) 249, 269
MboII GAAGA 4 cut(s) 123, 243, 289, 325
MhlI GDGCHC 1 cut(s) 284
MlsI TGGCCA 2 cut(s) 5, 65
MluCI AATT 7 cut(s) 99, 111, 154, 320, 371, 401, 479
MluNI TGGCCA 2 cut(s) 5, 65
MmeI TCCRAC 3 cut(s) 124, 439, 547
MnlI CCTC 3 cut(s) 97, 200, 553
Mox20I TGGCCA 2 cut(s) 5, 65
MscI TGGCCA 2 cut(s) 5, 65
Msp20I TGGCCA 2 cut(s) 5, 65
NlaIII CATG 2 cut(s) 70, 83
NlaIV GGNNCC 1 cut(s) 148
PciSI GCTCTTC 1 cut(s) 272
PfeI GAWTC 1 cut(s) 128
Pfl23II CGTACG 1 cut(s) 244
PflMI CCANNNNNTGG 1 cut(s) 265
PkrI GCNGC 2 cut(s) 369, 490
Psp1406I AACGTT 1 cut(s) 456
PspLI CGTACG 1 cut(s) 244
PspN4I GGNNCC 1 cut(s) 148
PstNI CAGNNNCTG 1 cut(s) 265
RsaI GTAC 2 cut(s) 172, 246
RsaNI GTAC 2 cut(s) 171, 245
SapI GCTCTTC 1 cut(s) 272
SatI GCNGC 2 cut(s) 368, 489
SduI GDGCHC 1 cut(s) 284
SetI ASST 5 cut(s) 76, 246, 292, 459, 501
SmlI CTYRAG 1 cut(s) 526
SmoI CTYRAG 1 cut(s) 526
Sse9I AATT 7 cut(s) 99, 111, 154, 320, 371, 401, 479
SsiI CCGC 1 cut(s) 174
SspI AATATT 1 cut(s) 430
SspMI CTAG 2 cut(s) 71, 577
StyI CCWWGG 1 cut(s) 351
TaaI ACNGT 1 cut(s) 249
TaiI ACGT 2 cut(s) 246, 459
TasI AATT 7 cut(s) 99, 111, 154, 320, 371, 401, 479
TfiI GAWTC 1 cut(s) 128
TscAI CASTG 1 cut(s) 578
TseI GCWGC 2 cut(s) 367, 488
TspGWI ACGGA 1 cut(s) 400
TspRI CASTG 1 cut(s) 578
Van91I CCANNNNNTGG 1 cut(s) 265
XspI CTAG 2 cut(s) 71, 577
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.