Rroxscaffold_2G00144370

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0000448
Physical Location & Seq
Reverse (-)
82271898 .. 82274072
2175 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00144370.1

Sequence Viewer

Length: 1125 bp
ATGGCAAAGGCAGCTGATCTGTTGCTGTGTGTCTCGTCATTTTCTTTGTCAGTTATCTTTCATACTTCTCTGGTCGTACTCGTAGCCGTTACTAATGCAGATGATCAGGTGGCTCCTAAACCTGCAGCAATTTTCATATTTGGAGACTCGACTGTGGATGTTGGCACCAACCAATGGTTGAACAATAGTCATTCGAGAGCTGATTTCTCTCCCAATGGAGTTGATTTTCCTTATTCTGTTCCGACGGGGAGGTTTAGTAATGGCCTTAACAGCGCTGACCAAATTGTAAGACTATTTGGGTACAAGAGAAGTCCGCAACCATTCTTGTATTCTCTGAACCATCAATCCACTTTCAAAAGAGATATACTGCAGGGAGTTAATTTTGCATCAGGAGGATCCGGCATATTCAACCAAACAGGAAAAAAGGACTATGGAGAAGTTGTGCCACTGGGAGATCAGGTCAAACAATTTGCGACGGTTCGTGGAAATATCACAGAACTAATTGGTCTTGGGGCAACTGAGATAATGTTTTCCAAGTCTTTGTTCGTTATTAGTATAGGAAGCAATGACCTGTTCGAGTTAGTGAATTGGTATCCAAATATCACTTCCTTGGTTAAGGATGAGTACTTGGAAACTGACTTATACAATCTGGGAGCTAGAAAATTCGGGATTATAAGTGTTCCTCCAATTGGATGCTGTCCTGCTGCACGTGTTGGACCAAAATCGAATGACTCCAGTGTTTGTGTAGAGGAACTAAACAATCTTGCCTTAACATTTCTTGCAAAAACTGAGGATCTCCTCCAAAAATTGAGCTTAGAGTTGAAAGGACTCATGTACTCGCTTGGAAATGCTTATGAAATGACTATGAGTATGCTTGAAGACCCATTAGCATTTGGCTTCAAGGACACTCAATCAGCCTGCTGTGGGTCAGGAAAGTTAAATGGTGTGTTTCCTTGCTTCTTCTTACTCGGTCCCAACCTTTGTCCGAATCGGCGGGAGGTTTTGTTCTGGGATTTGTATCATCCTACGGAATATGCTTCCGAGCTAGCAGCACTAACCCTTTATGGTGGAGGAACAAGATACGTCACACCTATAAACTTTAGTCAGTTGGTGGCTATAAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

374

Amino Acids

40.8

Weight (kDa)

5.32

Isoelectric Point (pI)

30.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 44 - 351 1.3e-24 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000448)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g10790 FvH4_1g10790 FvH4_1g10790 FvH4_1g10791 FvH4_1g10800 FvH4_2g15970 FvH4_2g15980 FvH4_2g15990
malus_domestica MD02G1121900.v1.1 MD05G1128700.v1.1 MD10G1131900.v1.1 MD15G1235500.v1.1 MD15G1235600.v1.1
prunus_persica Prupe.7G177500_v2.0.a1 Prupe.7G177600_v2.0.a1 Prupe.8G172800_v2.0.a1
pyrus_communis pycom05g12460 pycom05g12470 pycom10g11300
rosa_chinensis RchiOBHm_Chr2g0097971 RchiOBHm_Chr2g0098021 RchiOBHm_Chr2g0098061 RchiOBHm_Chr6g0257301 RchiOBHm_Chr6g0279511 RchiOBHm_Chr6g0279521
rosa_laevigata RLG00000013139 RLG00000013140 RLG00000013141 RLG00000016747 RLG00000016749 RLG00000016750 RLG00000016751 RLG00000016752
rosa_multiflora Rmu_sc0002648.1_g000005 Rmu_sc0002648.1_g000006 Rmu_sc0004649.1_g000019 Rmu_sc0004649.1_g000021 Rmu_sc0009572.1_g000002 Rmu_sc0013919.1_g000008 Rmu_sc0013919.1_g000009
rosa_roxburghii Rroxscaffold_2G00144370 Rroxscaffold_2G00144380 Rroxscaffold_2G00144390 Rroxscaffold_2G00144410 Rroxscaffold_4G00294600 Rroxscaffold_7G00188840 Rroxscaffold_7G00188850 Rroxscaffold_7G00188860
rosa_rugosa Rorug02G0069400 Rorug02G0069600 Rorug02G0069700 Rorug02G0069800 Rorug06G0098000.1 Rorug06G0125000 Rorug06G0125100 Rorug06G0125200
rosa_samantha Rh2AG116400 Rh2AG116800 Rh2AG116900 Rh2AG117000 Rh2BG119300 Rh2BG119500 Rh2BG119600 Rh2BG119700 Rh2BG119800 Rh2CG120900 Rh2CG121000 Rh2CG121200 Rh2CG121300 Rh2CG121400 Rh2CG121500 Rh2DG120600 Rh2DG121100 Rh2DG121200 Rh2DG121500 Rh6AG091200 Rh6AG234100 Rh6BG238400 Rh6BG238500 Rh6BG238600 Rh6CG078800 Rh6CG240400 Rh6CG240500 Rh6CG240600 Rh6DG074600 Rh6DG232000 Rh6DG232100
rosa_wichuraiana Rw2G009120 Rw2G009130 Rw2G009140 Rw6G020420 Rw6G020430 Rw6G020440

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 674
Acc36I ACCTGC 1 cut(s) 130
AccB1I GGYRCC 1 cut(s) 164
AccB7I CCANNNNNTGG 1 cut(s) 174
AciI CCGC 2 cut(s) 314, 994
AclWI GGATC 3 cut(s) 390, 403, 801
AcsI RAATTY 1 cut(s) 662
AcvI CACGTG 1 cut(s) 710
AfaI GTAC 4 cut(s) 78, 302, 626, 836
AfeI AGCGCT 1 cut(s) 274
AfiI CCNNNNNNNGG 3 cut(s) 174, 689, 924
AflIII ACRYGT 1 cut(s) 709
AgsI TTSAA 6 cut(s) 181, 355, 409, 823, 878, 901
AjuI GAANNNNNNNTTGG 4 cut(s) 527, 559, 589, 621
AloI GAACNNNNNNTCC 4 cut(s) 329, 361, 989, 1021
AluBI AGCT 5 cut(s) 14, 200, 656, 813, 1045
AluI AGCT 5 cut(s) 14, 200, 656, 813, 1045
Alw26I GTCTC 2 cut(s) 37, 138
AlwI GGATC 3 cut(s) 390, 403, 801
Aor51HI AGCGCT 1 cut(s) 274
AoxI GGCC 1 cut(s) 262
ApeKI GCWGC 4 cut(s) 11, 125, 704, 1049
ApoI RAATTY 1 cut(s) 662
AspLEI GCGC 1 cut(s) 275
AspS9I GGNCC 2 cut(s) 716, 971
AsuNHI GCTAGC 1 cut(s) 1045
AvaII GGWCC 2 cut(s) 716, 971
BamHI GGATCC 1 cut(s) 395
BanI GGYRCC 1 cut(s) 164
BbrPI CACGTG 1 cut(s) 710
BbsI GAAGAC 1 cut(s) 885
BbvI GCAGC 4 cut(s) 23, 137, 691, 1061
BccI CCATC 1 cut(s) 348
BceAI ACGGC 1 cut(s) 71
BciVI GTATCC 1 cut(s) 603
BclI TGATCA 1 cut(s) 103
BcoDI GTCTC 2 cut(s) 37, 138
BfaI CTAG 2 cut(s) 657, 1046
BfmI CTRYAG 2 cut(s) 123, 368
BfoI RGCGCY 1 cut(s) 276
BfuAI ACCTGC 1 cut(s) 130
BfuI GTATCC 1 cut(s) 603
BisI GCNGC 4 cut(s) 12, 126, 705, 1050
BlsI GCNGC 4 cut(s) 13, 127, 706, 1051
BmcAI AGTACT 1 cut(s) 626
Bme18I GGWCC 2 cut(s) 716, 971
BmgT120I GGNCC 2 cut(s) 716, 971
BmiI GGNNCC 4 cut(s) 114, 166, 397, 973
BmrI ACTGGG 1 cut(s) 458
BmsI GCATC 2 cut(s) 395, 683
BmtI GCTAGC 1 cut(s) 1049
BmuI ACTGGG 1 cut(s) 458
BpiI GAAGAC 1 cut(s) 885
BpmI CTGGAG 1 cut(s) 718
BsaAI YACGTR 1 cut(s) 710
BsaBI GATNNNNATC 1 cut(s) 1017
BsaJI CCNNGG 1 cut(s) 609
BsaXI ACNNNNNCTCC 8 cut(s) 241, 271, 426, 444, 456, 474, 989, 1019
Bsc4I CCNNNNNNNGG 3 cut(s) 174, 689, 924
Bse1I ACTGG 2 cut(s) 453, 735
Bse3DI GCAATG 1 cut(s) 571
Bse8I GATNNNNATC 1 cut(s) 1017
BseDI CCNNGG 1 cut(s) 609
BseGI GGATG 4 cut(s) 163, 625, 698, 1021
BseJI GATNNNNATC 1 cut(s) 1017
BseLI CCNNNNNNNGG 3 cut(s) 174, 689, 924
BseMI GCAATG 1 cut(s) 571
BseMII CTCAG 2 cut(s) 510, 780
BseNI ACTGG 2 cut(s) 453, 735
BseRI GAGGAG 1 cut(s) 788
BseXI GCAGC 4 cut(s) 23, 137, 691, 1061
BsgI GTGCAG 1 cut(s) 690
BshFI GGCC 1 cut(s) 264
BshNI GGYRCC 1 cut(s) 164
BsiSI CCGG 1 cut(s) 399
BslFI GGGAC 1 cut(s) 957
BslI CCNNNNNNNGG 3 cut(s) 174, 689, 924
BsmAI GTCTC 2 cut(s) 37, 138
BsmFI GGGAC 1 cut(s) 957
BsnI GGCC 1 cut(s) 264
Bsp143I GATC 5 cut(s) 16, 103, 395, 454, 793
BspACI CCGC 2 cut(s) 314, 994
BspANI GGCC 1 cut(s) 264
BspCNI CTCAG 2 cut(s) 511, 781
BspLI GGNNCC 4 cut(s) 114, 166, 397, 973
BspMAI CTGCAG 2 cut(s) 127, 372
BspMI ACCTGC 1 cut(s) 130
BspOI GCTAGC 1 cut(s) 1049
BspPI GGATC 3 cut(s) 390, 403, 801
BspT107I GGYRCC 1 cut(s) 164
BsrDI GCAATG 1 cut(s) 571
BsrI ACTGG 2 cut(s) 453, 735
BssECI CCNNGG 1 cut(s) 609
BssMI GATC 5 cut(s) 16, 103, 395, 454, 793
BssT1I CCWWGG 1 cut(s) 609
Bst4CI ACNGT 2 cut(s) 154, 478
BstBAI YACGTR 1 cut(s) 710
BstC8I GCNNGC 2 cut(s) 919, 1047
BstDEI CTNAG 3 cut(s) 519, 789, 814
BstF5I GGATG 4 cut(s) 163, 625, 698, 1021
BstH2I RGCGCY 1 cut(s) 276
BstHHI GCGC 1 cut(s) 275
BstKTI GATC 5 cut(s) 19, 106, 398, 457, 796
BstMAI GTCTC 2 cut(s) 37, 138
BstMBI GATC 5 cut(s) 16, 103, 395, 454, 793
BstMWI GCNNNNNNNGC 2 cut(s) 11, 270
BstSFI CTRYAG 2 cut(s) 123, 368
BstV1I GCAGC 4 cut(s) 23, 137, 691, 1061
BstV2I GAAGAC 1 cut(s) 885
BstX2I RGATCY 2 cut(s) 395, 793
BstYI RGATCY 2 cut(s) 395, 793
BsuI GTATCC 1 cut(s) 603
BsuRI GGCC 1 cut(s) 264
BtsCI GGATG 4 cut(s) 163, 625, 698, 1021
BtsIMutI CAGTG 2 cut(s) 446, 742
BveI ACCTGC 1 cut(s) 130
Cac8I GCNNGC 2 cut(s) 919, 1047
CfoI GCGC 1 cut(s) 275
Cfr13I GGNCC 2 cut(s) 716, 971
Csp6I GTAC 4 cut(s) 77, 301, 625, 835
CviAII CATG 1 cut(s) 832
CviQI GTAC 4 cut(s) 77, 301, 625, 835
DdeI CTNAG 3 cut(s) 519, 789, 814
DpnI GATC 5 cut(s) 18, 105, 397, 456, 795
DpnII GATC 5 cut(s) 16, 103, 395, 454, 793
Eco130I CCWWGG 1 cut(s) 609
Eco47I GGWCC 2 cut(s) 716, 971
Eco47III AGCGCT 1 cut(s) 274
Eco72I CACGTG 1 cut(s) 710
EcoT14I CCWWGG 1 cut(s) 609
ErhI CCWWGG 1 cut(s) 609
FaeI CATG 1 cut(s) 835
FaqI GGGAC 1 cut(s) 957
FatI CATG 1 cut(s) 831
FauI CCCGC 1 cut(s) 987
FbaI TGATCA 1 cut(s) 103
Fnu4HI GCNGC 4 cut(s) 12, 126, 705, 1050
FokI GGATG 4 cut(s) 170, 632, 705, 1008
Fsp4HI GCNGC 4 cut(s) 12, 126, 705, 1050
FspBI CTAG 2 cut(s) 657, 1046
GlaI GCGC 1 cut(s) 274
GluI GCNGC 4 cut(s) 12, 126, 705, 1050
GsuI CTGGAG 1 cut(s) 718
HaeII RGCGCY 1 cut(s) 276
HaeIII GGCC 1 cut(s) 264
HapII CCGG 1 cut(s) 399
HhaI GCGC 1 cut(s) 275
Hin1II CATG 1 cut(s) 835
Hin6I GCGC 1 cut(s) 273
HinP1I GCGC 1 cut(s) 273
HinfI GANTC 4 cut(s) 146, 731, 828, 988
HpaII CCGG 1 cut(s) 399
Hpy188I TCNGA 4 cut(s) 243, 336, 987, 1042
Hpy188III TCNNGA 4 cut(s) 195, 390, 667, 930
Hpy99I CGWCG 2 cut(s) 247, 478
HpyCH4III ACNGT 2 cut(s) 154, 478
HpyCH4IV ACGT 2 cut(s) 709, 1083
HpyCH4V TGCA 6 cut(s) 98, 125, 370, 386, 707, 782
HpyF10VI GCNNNNNNNGC 2 cut(s) 11, 270
HpyF3I CTNAG 3 cut(s) 519, 789, 814
HpySE526I ACGT 2 cut(s) 709, 1083
Hsp92II CATG 1 cut(s) 835
HspAI GCGC 1 cut(s) 273
Ksp22I TGATCA 1 cut(s) 103
Kzo9I GATC 5 cut(s) 16, 103, 395, 454, 793
LmnI GCTCC 2 cut(s) 118, 653
Lsp1109I GCAGC 4 cut(s) 23, 137, 691, 1061
LweI GCATC 2 cut(s) 395, 683
MaeI CTAG 2 cut(s) 657, 1046
MaeII ACGT 2 cut(s) 709, 1083
MaeIII GTNAC 2 cut(s) 88, 1084
MalI GATC 5 cut(s) 18, 105, 397, 456, 795
MboI GATC 5 cut(s) 16, 103, 395, 454, 793
MboII GAAGA 2 cut(s) 890, 952
MfeI CAATTG 1 cut(s) 687
MflI RGATCY 2 cut(s) 395, 793
MluCI AATT 9 cut(s) 129, 282, 379, 467, 501, 586, 662, 687, 806
MlyI GAGTC 3 cut(s) 140, 725, 822
MmeI TCCRAC 2 cut(s) 266, 694
MnlI CCTC 8 cut(s) 243, 386, 693, 742, 784, 809, 991, 1064
MseI TTAA 5 cut(s) 267, 378, 615, 770, 938
MspA1I CMGCKG 1 cut(s) 14
MspI CCGG 1 cut(s) 399
MunI CAATTG 1 cut(s) 687
MwoI GCNNNNNNNGC 2 cut(s) 11, 270
NdeII GATC 5 cut(s) 16, 103, 395, 454, 793
NheI GCTAGC 1 cut(s) 1045
NlaIII CATG 1 cut(s) 835
NlaIV GGNNCC 4 cut(s) 114, 166, 397, 973
NmuCI GTSAC 1 cut(s) 1084
PfeI GAWTC 1 cut(s) 988
PflMI CCANNNNNTGG 1 cut(s) 174
PkrI GCNGC 4 cut(s) 13, 127, 706, 1051
PleI GAGTC 3 cut(s) 140, 725, 822
PmaCI CACGTG 1 cut(s) 710
PmlI CACGTG 1 cut(s) 710
PpsI GAGTC 3 cut(s) 140, 725, 822
Ppu21I YACGTR 1 cut(s) 710
PsiI TTATAA 1 cut(s) 674
PspCI CACGTG 1 cut(s) 710
PspN4I GGNNCC 4 cut(s) 114, 166, 397, 973
PspPI GGNCC 2 cut(s) 716, 971
PstI CTGCAG 2 cut(s) 127, 372
PsuI RGATCY 2 cut(s) 395, 793
PvuII CAGCTG 1 cut(s) 14
RsaI GTAC 4 cut(s) 78, 302, 626, 836
RsaNI GTAC 4 cut(s) 77, 301, 625, 835
SaqAI TTAA 5 cut(s) 267, 378, 615, 770, 938
SatI GCNGC 4 cut(s) 12, 126, 705, 1050
Sau3AI GATC 5 cut(s) 16, 103, 395, 454, 793
Sau96I GGNCC 2 cut(s) 716, 971
ScaI AGTACT 1 cut(s) 626
SchI GAGTC 3 cut(s) 140, 725, 822
SfaNI GCATC 2 cut(s) 395, 683
SfcI CTRYAG 2 cut(s) 123, 368
SinI GGWCC 2 cut(s) 716, 971
Sse9I AATT 9 cut(s) 129, 282, 379, 467, 501, 586, 662, 687, 806
SsiI CCGC 2 cut(s) 314, 994
SspMI CTAG 2 cut(s) 657, 1046
StyI CCWWGG 1 cut(s) 609
TaaI ACNGT 2 cut(s) 154, 478
TaiI ACGT 2 cut(s) 712, 1086
TaqI TCGA 4 cut(s) 149, 194, 576, 725
TaqII GACCGA 1 cut(s) 959
TasI AATT 9 cut(s) 129, 282, 379, 467, 501, 586, 662, 687, 806
TatI WGTACW 2 cut(s) 624, 834
TfiI GAWTC 1 cut(s) 988
Tru1I TTAA 5 cut(s) 267, 378, 615, 770, 938
Tru9I TTAA 5 cut(s) 267, 378, 615, 770, 938
TscAI CASTG 2 cut(s) 453, 742
TseFI GTSAC 1 cut(s) 1084
TseI GCWGC 4 cut(s) 11, 125, 704, 1049
Tsp45I GTSAC 1 cut(s) 1084
TspDTI ATGAA 3 cut(s) 50, 124, 870
TspGWI ACGGA 1 cut(s) 1043
TspRI CASTG 2 cut(s) 453, 742
Van91I CCANNNNNTGG 1 cut(s) 174
VpaK11BI GGWCC 2 cut(s) 716, 971
XapI RAATTY 1 cut(s) 662
XspI CTAG 2 cut(s) 657, 1046
ZrmI AGTACT 1 cut(s) 626
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.