Rh2CG121500

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2C
OG0000448
Physical Location & Seq
Forward (+)
10782574 .. 10800507
17934 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2CG121500.1

Sequence Viewer

Length: 1119 bp
ATGAGGTATTCACTTGGAAATGCCTATGAAATGACCATGAGTATCATTCAAGACCCATTAGCCTTTGATGATCAGGTGGCTCCTAAACCTGCAGCAATTTTCATATTTGGAGACTCGACTGTGGATGTTGGCACCAACCAATGGTTGAACAATAGTTATTCGAGAGCTGATTTTTCTCCCAATGGAGTTGATTTTCCTTATTCTGTTCCGACGGGGAGGTTTAGTAATGGCCTTAACAGCGCTGACCAAATTGTAAGACTATTTGGGTACAAGAGAAGTCCGCAACCATTCTTGTATTCTCTGAACCATCAATCCACTTTCAAAAGAGATATACTGCAGGGAGTTAATTTTGCATCCGGAGGATCCGGCATATTCAACCAAACAGGAAAAAAGGACTATGGAGAAGTTGTGCCACTGGGAGATCAGGTCAAACAATTTGCGACGGTTCGTGGAAATATCACAGAACTAATTGGTCTTGGGGCAACTGAGATAATGTTTTCCAAGTCTTTGTTCGTTATTAGTATAGGAAGCAATGACCTGTTCGAGTTAGTGAAATGGCATCCAAATATCACTTCCTTGGCTAAGGATGAGTACTTGGAAACTCTTAAACTCAACTACAGTAACCATCTGAAGGACTTATACAATCTGGGAGCAAGAAAATTCGGGATTATAAGTGTTCCTCCAATTGGATGCTGTCCTGCTGCACGTGTTGGACCAAAATCGAATGACTCCAGTGTTTGTGTAGAGGAACTAAACAATCTTGCCTTAACATTTCTTGCAAAAACTGAGGATCTCCTCCAAAAATTGAGCTCAGAGTTGAAAGGACTCATGTACTCGCTTGGAAATGCTTATGAAATGACTATGAGTATACTTGAAGACCCATTGGCGTTTGGCTTCAAGGACACTCACTCAGCCTGCTGCTGGTCAGGAAAGTTAAATGGAGTGTTTCCTTGCTTCTTCTTACTAGGTCCCAACCTTTGTCCGAATCGGCGGGAGGTTTTGTTCTGGGATTTGTATCATCCTACGGAATATGCTTCCGAGCTAGCAGCACTAACCCTTTATGGTGGAGGAACAAGATACGTCACACCTATAAACTTCAGTCAGTTGGGGTGGCTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

372

Amino Acids

41.17

Weight (kDa)

5.61

Isoelectric Point (pI)

34.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 33 - 350 5.4e-27 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000448)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g10790 FvH4_1g10790 FvH4_1g10790 FvH4_1g10791 FvH4_1g10800 FvH4_2g15970 FvH4_2g15980 FvH4_2g15990
malus_domestica MD02G1121900.v1.1 MD05G1128700.v1.1 MD10G1131900.v1.1 MD15G1235500.v1.1 MD15G1235600.v1.1
prunus_persica Prupe.7G177500_v2.0.a1 Prupe.7G177600_v2.0.a1 Prupe.8G172800_v2.0.a1
pyrus_communis pycom05g12460 pycom05g12470 pycom10g11300
rosa_chinensis RchiOBHm_Chr2g0097971 RchiOBHm_Chr2g0098021 RchiOBHm_Chr2g0098061 RchiOBHm_Chr6g0257301 RchiOBHm_Chr6g0279511 RchiOBHm_Chr6g0279521
rosa_laevigata RLG00000013139 RLG00000013140 RLG00000013141 RLG00000016747 RLG00000016749 RLG00000016750 RLG00000016751 RLG00000016752
rosa_multiflora Rmu_sc0002648.1_g000005 Rmu_sc0002648.1_g000006 Rmu_sc0004649.1_g000019 Rmu_sc0004649.1_g000021 Rmu_sc0009572.1_g000002 Rmu_sc0013919.1_g000008 Rmu_sc0013919.1_g000009
rosa_roxburghii Rroxscaffold_2G00144370 Rroxscaffold_2G00144380 Rroxscaffold_2G00144390 Rroxscaffold_2G00144410 Rroxscaffold_4G00294600 Rroxscaffold_7G00188840 Rroxscaffold_7G00188850 Rroxscaffold_7G00188860
rosa_rugosa Rorug02G0069400 Rorug02G0069600 Rorug02G0069700 Rorug02G0069800 Rorug06G0098000.1 Rorug06G0125000 Rorug06G0125100 Rorug06G0125200
rosa_samantha Rh2AG116400 Rh2AG116800 Rh2AG116900 Rh2AG117000 Rh2BG119300 Rh2BG119500 Rh2BG119600 Rh2BG119700 Rh2BG119800 Rh2CG120900 Rh2CG121000 Rh2CG121200 Rh2CG121300 Rh2CG121400 Rh2CG121500 Rh2DG120600 Rh2DG121100 Rh2DG121200 Rh2DG121500 Rh6AG091200 Rh6AG234100 Rh6BG238400 Rh6BG238500 Rh6BG238600 Rh6CG078800 Rh6CG240400 Rh6CG240500 Rh6CG240600 Rh6DG074600 Rh6DG232000 Rh6DG232100
rosa_wichuraiana Rw2G009120 Rw2G009130 Rw2G009140 Rw6G020420 Rw6G020430 Rw6G020440

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 671
Acc36I ACCTGC 1 cut(s) 97
AccB1I GGYRCC 1 cut(s) 131
AccB7I CCANNNNNTGG 1 cut(s) 141
AccI GTMKAC 1 cut(s) 868
AccIII TCCGGA 1 cut(s) 356
AciI CCGC 2 cut(s) 281, 991
AclWI GGATC 3 cut(s) 357, 370, 798
AcsI RAATTY 1 cut(s) 659
AcuI CTGAAG 2 cut(s) 650, 1081
AcvI CACGTG 1 cut(s) 707
AfaI GTAC 3 cut(s) 269, 593, 833
AfeI AGCGCT 1 cut(s) 241
AfiI CCNNNNNNNGG 4 cut(s) 141, 631, 686, 921
AflIII ACRYGT 1 cut(s) 706
AgsI TTSAA 7 cut(s) 50, 148, 322, 376, 820, 875, 898
AjuI GAANNNNNNNTTGG 4 cut(s) 494, 526, 556, 588
AloI GAACNNNNNNTCC 4 cut(s) 296, 328, 986, 1018
AluBI AGCT 3 cut(s) 167, 810, 1042
AluI AGCT 3 cut(s) 167, 810, 1042
Alw21I GWGCWC 1 cut(s) 812
Alw26I GTCTC 1 cut(s) 105
AlwI GGATC 3 cut(s) 357, 370, 798
Aor13HI TCCGGA 1 cut(s) 356
Aor51HI AGCGCT 1 cut(s) 241
AoxI GGCC 1 cut(s) 229
ApeKI GCWGC 4 cut(s) 92, 701, 918, 1046
ApoI RAATTY 1 cut(s) 659
AspLEI GCGC 1 cut(s) 242
AspS9I GGNCC 2 cut(s) 713, 968
AsuNHI GCTAGC 1 cut(s) 1042
AvaII GGWCC 2 cut(s) 713, 968
BamHI GGATCC 1 cut(s) 362
BanI GGYRCC 1 cut(s) 131
BanII GRGCYC 1 cut(s) 812
BarI GAAGNNNNNNTAC 2 cut(s) 623, 655
BbrPI CACGTG 1 cut(s) 707
BbsI GAAGAC 1 cut(s) 882
Bbv12I GWGCWC 1 cut(s) 812
BbvI GCAGC 4 cut(s) 104, 688, 905, 1058
BccI CCATC 2 cut(s) 315, 633
BclI TGATCA 1 cut(s) 70
BcoDI GTCTC 1 cut(s) 105
BfaI CTAG 2 cut(s) 965, 1043
BfmI CTRYAG 3 cut(s) 90, 335, 616
BfoI RGCGCY 1 cut(s) 243
BfuAI ACCTGC 1 cut(s) 97
BisI GCNGC 4 cut(s) 93, 702, 919, 1047
BlsI GCNGC 4 cut(s) 94, 703, 920, 1048
BmcAI AGTACT 1 cut(s) 593
Bme18I GGWCC 2 cut(s) 713, 968
BmgT120I GGNCC 2 cut(s) 713, 968
BmiI GGNNCC 4 cut(s) 81, 133, 364, 970
BmrI ACTGGG 1 cut(s) 425
BmsI GCATC 3 cut(s) 362, 568, 680
BmtI GCTAGC 1 cut(s) 1046
BmuI ACTGGG 1 cut(s) 425
BpiI GAAGAC 1 cut(s) 882
BpmI CTGGAG 1 cut(s) 715
Bpu10I CCTNAGC 1 cut(s) 582
BsaAI YACGTR 1 cut(s) 707
BsaBI GATNNNNATC 1 cut(s) 1014
BsaJI CCNNGG 1 cut(s) 576
BsaWI WCCGGW 1 cut(s) 356
BsaXI ACNNNNNCTCC 8 cut(s) 208, 238, 393, 411, 423, 441, 986, 1016
Bsc4I CCNNNNNNNGG 4 cut(s) 141, 631, 686, 921
Bse1I ACTGG 2 cut(s) 420, 732
Bse3DI GCAATG 1 cut(s) 538
Bse8I GATNNNNATC 1 cut(s) 1014
BseAI TCCGGA 1 cut(s) 356
BseDI CCNNGG 1 cut(s) 576
BseGI GGATG 6 cut(s) 130, 353, 559, 592, 695, 1018
BseJI GATNNNNATC 1 cut(s) 1014
BseLI CCNNNNNNNGG 4 cut(s) 141, 631, 686, 921
BseMI GCAATG 1 cut(s) 538
BseMII CTCAG 4 cut(s) 477, 777, 825, 924
BseNI ACTGG 2 cut(s) 420, 732
BseRI GAGGAG 1 cut(s) 785
BseXI GCAGC 4 cut(s) 104, 688, 905, 1058
BsgI GTGCAG 1 cut(s) 687
BshFI GGCC 1 cut(s) 231
BshNI GGYRCC 1 cut(s) 131
BsiHKAI GWGCWC 1 cut(s) 812
BsiSI CCGG 2 cut(s) 357, 366
BslFI GGGAC 1 cut(s) 954
BslI CCNNNNNNNGG 4 cut(s) 141, 631, 686, 921
BsmAI GTCTC 1 cut(s) 105
BsmFI GGGAC 1 cut(s) 954
BsnI GGCC 1 cut(s) 231
Bsp1286I GDGCHC 1 cut(s) 812
Bsp13I TCCGGA 1 cut(s) 356
Bsp143I GATC 4 cut(s) 70, 362, 421, 790
BspACI CCGC 2 cut(s) 281, 991
BspANI GGCC 1 cut(s) 231
BspCNI CTCAG 4 cut(s) 478, 778, 824, 923
BspEI TCCGGA 1 cut(s) 356
BspLI GGNNCC 4 cut(s) 81, 133, 364, 970
BspMAI CTGCAG 2 cut(s) 94, 339
BspMI ACCTGC 1 cut(s) 97
BspOI GCTAGC 1 cut(s) 1046
BspPI GGATC 3 cut(s) 357, 370, 798
BspT107I GGYRCC 1 cut(s) 131
BsrDI GCAATG 1 cut(s) 538
BsrI ACTGG 2 cut(s) 420, 732
BssECI CCNNGG 1 cut(s) 576
BssMI GATC 4 cut(s) 70, 362, 421, 790
BssNAI GTATAC 1 cut(s) 869
BssT1I CCWWGG 1 cut(s) 576
Bst1107I GTATAC 1 cut(s) 869
Bst4CI ACNGT 3 cut(s) 121, 445, 620
BstBAI YACGTR 1 cut(s) 707
BstC8I GCNNGC 2 cut(s) 916, 1044
BstDEI CTNAG 5 cut(s) 486, 582, 786, 811, 910
BstF5I GGATG 6 cut(s) 130, 353, 559, 592, 695, 1018
BstH2I RGCGCY 1 cut(s) 243
BstHHI GCGC 1 cut(s) 242
BstKTI GATC 4 cut(s) 73, 365, 424, 793
BstMAI GTCTC 1 cut(s) 105
BstMBI GATC 4 cut(s) 70, 362, 421, 790
BstMWI GCNNNNNNNGC 1 cut(s) 237
BstSFI CTRYAG 3 cut(s) 90, 335, 616
BstV1I GCAGC 4 cut(s) 104, 688, 905, 1058
BstV2I GAAGAC 1 cut(s) 882
BstX2I RGATCY 2 cut(s) 362, 790
BstYI RGATCY 2 cut(s) 362, 790
BstZ17I GTATAC 1 cut(s) 869
BsuRI GGCC 1 cut(s) 231
BtsCI GGATG 6 cut(s) 130, 353, 559, 592, 695, 1018
BtsIMutI CAGTG 2 cut(s) 413, 739
BveI ACCTGC 1 cut(s) 97
Cac8I GCNNGC 2 cut(s) 916, 1044
CfoI GCGC 1 cut(s) 242
Cfr13I GGNCC 2 cut(s) 713, 968
Csp6I GTAC 3 cut(s) 268, 592, 832
CviAII CATG 2 cut(s) 37, 829
CviQI GTAC 3 cut(s) 268, 592, 832
DdeI CTNAG 5 cut(s) 486, 582, 786, 811, 910
DpnI GATC 4 cut(s) 72, 364, 423, 792
DpnII GATC 4 cut(s) 70, 362, 421, 790
Ecl136II GAGCTC 1 cut(s) 810
Eco130I CCWWGG 1 cut(s) 576
Eco24I GRGCYC 1 cut(s) 812
Eco47I GGWCC 2 cut(s) 713, 968
Eco47III AGCGCT 1 cut(s) 241
Eco53kI GAGCTC 1 cut(s) 810
Eco57I CTGAAG 2 cut(s) 650, 1081
Eco72I CACGTG 1 cut(s) 707
EcoICRI GAGCTC 1 cut(s) 810
EcoO109I RGGNCCY 1 cut(s) 968
EcoT14I CCWWGG 1 cut(s) 576
EcoT38I GRGCYC 1 cut(s) 812
ErhI CCWWGG 1 cut(s) 576
FaeI CATG 2 cut(s) 40, 832
FaqI GGGAC 1 cut(s) 954
FatI CATG 2 cut(s) 36, 828
FauI CCCGC 1 cut(s) 984
FbaI TGATCA 1 cut(s) 70
FblI GTMKAC 1 cut(s) 868
Fnu4HI GCNGC 4 cut(s) 93, 702, 919, 1047
FokI GGATG 6 cut(s) 137, 340, 546, 599, 702, 1005
FriOI GRGCYC 1 cut(s) 812
Fsp4HI GCNGC 4 cut(s) 93, 702, 919, 1047
FspBI CTAG 2 cut(s) 965, 1043
GlaI GCGC 1 cut(s) 241
GluI GCNGC 4 cut(s) 93, 702, 919, 1047
GsuI CTGGAG 1 cut(s) 715
HaeII RGCGCY 1 cut(s) 243
HaeIII GGCC 1 cut(s) 231
HapII CCGG 2 cut(s) 357, 366
HhaI GCGC 1 cut(s) 242
Hin1II CATG 2 cut(s) 40, 832
Hin6I GCGC 1 cut(s) 240
HinP1I GCGC 1 cut(s) 240
HinfI GANTC 4 cut(s) 113, 728, 825, 985
HpaII CCGG 2 cut(s) 357, 366
Hpy166II GTNNAC 1 cut(s) 869
Hpy188I TCNGA 6 cut(s) 210, 303, 630, 814, 984, 1039
Hpy188III TCNNGA 5 cut(s) 50, 162, 357, 664, 927
Hpy8I GTNNAC 1 cut(s) 869
Hpy99I CGWCG 2 cut(s) 214, 445
HpyAV CCTTC 1 cut(s) 625
HpyCH4III ACNGT 3 cut(s) 121, 445, 620
HpyCH4IV ACGT 2 cut(s) 706, 1080
HpyCH4V TGCA 5 cut(s) 92, 337, 353, 704, 779
HpyF10VI GCNNNNNNNGC 1 cut(s) 237
HpyF3I CTNAG 5 cut(s) 486, 582, 786, 811, 910
HpySE526I ACGT 2 cut(s) 706, 1080
Hsp92II CATG 2 cut(s) 40, 832
HspAI GCGC 1 cut(s) 240
Kpn2I TCCGGA 1 cut(s) 356
Ksp22I TGATCA 1 cut(s) 70
Kzo9I GATC 4 cut(s) 70, 362, 421, 790
LmnI GCTCC 2 cut(s) 85, 650
Lsp1109I GCAGC 4 cut(s) 104, 688, 905, 1058
LweI GCATC 3 cut(s) 362, 568, 680
MaeI CTAG 2 cut(s) 965, 1043
MaeII ACGT 2 cut(s) 706, 1080
MaeIII GTNAC 2 cut(s) 620, 1081
MalI GATC 4 cut(s) 72, 364, 423, 792
MboI GATC 4 cut(s) 70, 362, 421, 790
MboII GAAGA 2 cut(s) 887, 949
MfeI CAATTG 1 cut(s) 684
MflI RGATCY 2 cut(s) 362, 790
MhlI GDGCHC 1 cut(s) 812
MluCI AATT 8 cut(s) 96, 249, 346, 434, 468, 659, 684, 803
MlyI GAGTC 3 cut(s) 107, 722, 819
MmeI TCCRAC 2 cut(s) 233, 691
MnlI CCTC 8 cut(s) 210, 353, 690, 739, 781, 806, 988, 1061
MroI TCCGGA 1 cut(s) 356
MseI TTAA 5 cut(s) 234, 345, 606, 767, 935
MspI CCGG 2 cut(s) 357, 366
MunI CAATTG 1 cut(s) 684
MwoI GCNNNNNNNGC 1 cut(s) 237
NdeII GATC 4 cut(s) 70, 362, 421, 790
NheI GCTAGC 1 cut(s) 1042
NlaIII CATG 2 cut(s) 40, 832
NlaIV GGNNCC 4 cut(s) 81, 133, 364, 970
NmuCI GTSAC 1 cut(s) 1081
PfeI GAWTC 1 cut(s) 985
PflMI CCANNNNNTGG 1 cut(s) 141
PkrI GCNGC 4 cut(s) 94, 703, 920, 1048
PleI GAGTC 3 cut(s) 107, 722, 819
PmaCI CACGTG 1 cut(s) 707
PmlI CACGTG 1 cut(s) 707
PpsI GAGTC 3 cut(s) 107, 722, 819
Ppu21I YACGTR 1 cut(s) 707
PpuMI RGGWCCY 1 cut(s) 968
PsiI TTATAA 1 cut(s) 671
Psp124BI GAGCTC 1 cut(s) 812
Psp5II RGGWCCY 1 cut(s) 968
PspCI CACGTG 1 cut(s) 707
PspN4I GGNNCC 4 cut(s) 81, 133, 364, 970
PspPI GGNCC 2 cut(s) 713, 968
PspPPI RGGWCCY 1 cut(s) 968
PstI CTGCAG 2 cut(s) 94, 339
PsuI RGATCY 2 cut(s) 362, 790
RsaI GTAC 3 cut(s) 269, 593, 833
RsaNI GTAC 3 cut(s) 268, 592, 832
SacI GAGCTC 1 cut(s) 812
SaqAI TTAA 5 cut(s) 234, 345, 606, 767, 935
SatI GCNGC 4 cut(s) 93, 702, 919, 1047
Sau3AI GATC 4 cut(s) 70, 362, 421, 790
Sau96I GGNCC 2 cut(s) 713, 968
ScaI AGTACT 1 cut(s) 593
SchI GAGTC 3 cut(s) 107, 722, 819
SduI GDGCHC 1 cut(s) 812
SfaNI GCATC 3 cut(s) 362, 568, 680
SfcI CTRYAG 3 cut(s) 90, 335, 616
SinI GGWCC 2 cut(s) 713, 968
Sse9I AATT 8 cut(s) 96, 249, 346, 434, 468, 659, 684, 803
SsiI CCGC 2 cut(s) 281, 991
SspMI CTAG 2 cut(s) 965, 1043
SstI GAGCTC 1 cut(s) 812
StyI CCWWGG 1 cut(s) 576
TaaI ACNGT 3 cut(s) 121, 445, 620
TaiI ACGT 2 cut(s) 709, 1083
TaqI TCGA 4 cut(s) 116, 161, 543, 722
TasI AATT 8 cut(s) 96, 249, 346, 434, 468, 659, 684, 803
TatI WGTACW 2 cut(s) 591, 831
TfiI GAWTC 1 cut(s) 985
Tru1I TTAA 5 cut(s) 234, 345, 606, 767, 935
Tru9I TTAA 5 cut(s) 234, 345, 606, 767, 935
TscAI CASTG 2 cut(s) 420, 739
TseFI GTSAC 1 cut(s) 1081
TseI GCWGC 4 cut(s) 92, 701, 918, 1046
Tsp45I GTSAC 1 cut(s) 1081
TspDTI ATGAA 3 cut(s) 42, 91, 867
TspGWI ACGGA 1 cut(s) 1040
TspRI CASTG 2 cut(s) 420, 739
Van91I CCANNNNNTGG 1 cut(s) 141
VpaK11BI GGWCC 2 cut(s) 713, 968
XapI RAATTY 1 cut(s) 659
XmiI GTMKAC 1 cut(s) 868
XspI CTAG 2 cut(s) 965, 1043
ZrmI AGTACT 1 cut(s) 593
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.