RLG00000016750

GDSL esterase lipase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0000448
Physical Location & Seq
Forward (+)
9695148 .. 9696956
1809 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000016750

Sequence Viewer

Length: 969 bp
ATGGCCAATAACAATGGGTGGACACCCTTGTTTTCTGTTTGGCTCTTTGTGTTTGTATATCTGGCCATGCTAGGTTTCCATGCATCCTCTTCACAGCAAATTGTGCCTGCAATTTACATATTTGGAGATTCTACAGGAGATGTTGAAACCAATGATTACTTGCCTGATTGTACTGCAAGGCCTGATATCCTCTATAATGGGATTGACTACCCTCACTCTAAACCAACAGGAAGATTTAGCAACGGTTACAACGTCGTAGATTACATCGCCCAGTTTCAGGGTTACAAGAAGAGCGTGCCACCTTTTCTCTCTCTTTTAGACGAGAAGAAGCAATTTCCTAAGAGGAAGGTTCCAAATAAGGGAGTTAACTTTGCGTCAGGAGGATCAGGACTTCCGGATGATACTGGTAAACTACAGTGGTGCGTACAACAGGGAAATGTCATTTCCTTGGGGGAACAAGTGCAGCAATTCCAAATTTTACGGAACAAAATCTCAGAATTATTGGGTGCTGGTGGGTTGGCTAATATTTCTCAGTCTCTCTTTGTCATCAGCGTTGGAAGCAACGACATCTTTGAATTATTCACTAACTTATTTATGCTCGGAGCAAGGAAATTTGGGATTATCAGCGTCTCACAAATTGGGTGCTGCCCTGCCCAGCGAAAGCTCAGTGAAAGAGGCGGGTGTTTGGAGGCCATGAATGCTTATGCACACTTGTTCTATACGTCACTCCAGTCCTCTTGCAGAAGGTTGGTGGAGGTGAATAAGGCTAGTTGTGGTAAAGGGAAGTTGAATGCAGAATTTCCATGTGAACCAGATTCGAATTTGAGTGAAAATCGCCGTAGCTACTTGTTCTGGGATCAATACCATTCAACAGAGGCTGCGTCTGGAATCCTAGCAATATATCTGTTTGGTGGTGCTCCAGTTTTGGTGACACCCTTGAGTTTCGGTCAATTGGCCCTCCTTGCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

323

Amino Acids

35.35

Weight (kDa)

7.53

Isoelectric Point (pI)

40.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 38 - 193 5.6e-09 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000448)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g10790 FvH4_1g10790 FvH4_1g10790 FvH4_1g10791 FvH4_1g10800 FvH4_2g15970 FvH4_2g15980 FvH4_2g15990
malus_domestica MD02G1121900.v1.1 MD05G1128700.v1.1 MD10G1131900.v1.1 MD15G1235500.v1.1 MD15G1235600.v1.1
prunus_persica Prupe.7G177500_v2.0.a1 Prupe.7G177600_v2.0.a1 Prupe.8G172800_v2.0.a1
pyrus_communis pycom05g12460 pycom05g12470 pycom10g11300
rosa_chinensis RchiOBHm_Chr2g0097971 RchiOBHm_Chr2g0098021 RchiOBHm_Chr2g0098061 RchiOBHm_Chr6g0257301 RchiOBHm_Chr6g0279511 RchiOBHm_Chr6g0279521
rosa_laevigata RLG00000013139 RLG00000013140 RLG00000013141 RLG00000016747 RLG00000016749 RLG00000016750 RLG00000016751 RLG00000016752
rosa_multiflora Rmu_sc0002648.1_g000005 Rmu_sc0002648.1_g000006 Rmu_sc0004649.1_g000019 Rmu_sc0004649.1_g000021 Rmu_sc0009572.1_g000002 Rmu_sc0013919.1_g000008 Rmu_sc0013919.1_g000009
rosa_roxburghii Rroxscaffold_2G00144370 Rroxscaffold_2G00144380 Rroxscaffold_2G00144390 Rroxscaffold_2G00144410 Rroxscaffold_4G00294600 Rroxscaffold_7G00188840 Rroxscaffold_7G00188850 Rroxscaffold_7G00188860
rosa_rugosa Rorug02G0069400 Rorug02G0069600 Rorug02G0069700 Rorug02G0069800 Rorug06G0098000.1 Rorug06G0125000 Rorug06G0125100 Rorug06G0125200
rosa_samantha Rh2AG116400 Rh2AG116800 Rh2AG116900 Rh2AG117000 Rh2BG119300 Rh2BG119500 Rh2BG119600 Rh2BG119700 Rh2BG119800 Rh2CG120900 Rh2CG121000 Rh2CG121200 Rh2CG121300 Rh2CG121400 Rh2CG121500 Rh2DG120600 Rh2DG121100 Rh2DG121200 Rh2DG121500 Rh6AG091200 Rh6AG234100 Rh6BG238400 Rh6BG238500 Rh6BG238600 Rh6CG078800 Rh6CG240400 Rh6CG240500 Rh6CG240600 Rh6DG074600 Rh6DG232000 Rh6DG232100
rosa_wichuraiana Rw2G009120 Rw2G009130 Rw2G009140 Rw6G020420 Rw6G020430 Rw6G020440

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 394
AciI CCGC 1 cut(s) 678
AclWI GGATC 2 cut(s) 391, 864
AcoI YGGCCR 2 cut(s) 3, 63
AcsI RAATTY 4 cut(s) 474, 611, 797, 820
AfaI GTAC 2 cut(s) 172, 426
AfiI CCNNNNNNNGG 2 cut(s) 277, 359
AgsI TTSAA 4 cut(s) 146, 575, 790, 870
AluBI AGCT 2 cut(s) 664, 843
AluI AGCT 2 cut(s) 664, 843
Alw21I GWGCWC 1 cut(s) 919
Alw26I GTCTC 2 cut(s) 540, 634
AlwI GGATC 2 cut(s) 391, 864
AlwNI CAGNNNCTG 1 cut(s) 878
Aor13HI TCCGGA 1 cut(s) 394
AoxI GGCC 5 cut(s) 3, 63, 179, 690, 954
ApeKI GCWGC 3 cut(s) 463, 645, 878
ApoI RAATTY 4 cut(s) 474, 611, 797, 820
AspS9I GGNCC 1 cut(s) 955
AsuHPI GGTGA 2 cut(s) 769, 940
AsuII TTCGAA 1 cut(s) 818
BalI TGGCCA 2 cut(s) 5, 65
Bbv12I GWGCWC 1 cut(s) 919
BbvI GCAGC 3 cut(s) 475, 632, 865
BceAI ACGGC 1 cut(s) 822
BcoDI GTCTC 2 cut(s) 540, 634
BfaI CTAG 3 cut(s) 71, 768, 893
BfmI CTRYAG 2 cut(s) 132, 413
BisI GCNGC 3 cut(s) 464, 646, 879
BlsI GCNGC 3 cut(s) 465, 647, 880
BmgT120I GGNCC 1 cut(s) 955
BmiI GGNNCC 1 cut(s) 351
BmrI ACTGGG 1 cut(s) 265
BmsI GCATC 1 cut(s) 92
BmuI ACTGGG 1 cut(s) 265
BpmI CTGGAG 2 cut(s) 713, 903
Bpu14I TTCGAA 1 cut(s) 818
BpuEI CTTGAG 1 cut(s) 958
BsaJI CCNNGG 1 cut(s) 447
BsaWI WCCGGW 1 cut(s) 394
Bsc4I CCNNNNNNNGG 2 cut(s) 277, 359
Bse1I ACTGG 4 cut(s) 271, 409, 730, 920
BseAI TCCGGA 1 cut(s) 394
BseDI CCNNGG 1 cut(s) 447
BseGI GGATG 2 cut(s) 83, 403
BseLI CCNNNNNNNGG 2 cut(s) 277, 359
BseMII CTCAG 3 cut(s) 507, 545, 679
BseNI ACTGG 4 cut(s) 271, 409, 730, 920
BseXI GCAGC 3 cut(s) 475, 632, 865
BseYI CCCAGC 1 cut(s) 654
BsgI GTGCAG 1 cut(s) 482
BshFI GGCC 5 cut(s) 5, 65, 181, 692, 956
BsiHKAI GWGCWC 1 cut(s) 919
BsiSI CCGG 1 cut(s) 395
BslI CCNNNNNNNGG 2 cut(s) 277, 359
BsmAI GTCTC 2 cut(s) 540, 634
BsmBI CGTCTC 1 cut(s) 634
BsmI GAATGC 2 cut(s) 703, 796
BsnI GGCC 5 cut(s) 5, 65, 181, 692, 956
Bsp119I TTCGAA 1 cut(s) 818
Bsp1286I GDGCHC 1 cut(s) 919
Bsp13I TCCGGA 1 cut(s) 394
Bsp143I GATC 2 cut(s) 383, 856
BspACI CCGC 1 cut(s) 678
BspANI GGCC 5 cut(s) 5, 65, 181, 692, 956
BspCNI CTCAG 3 cut(s) 506, 544, 678
BspEI TCCGGA 1 cut(s) 394
BspLI GGNNCC 1 cut(s) 351
BspPI GGATC 2 cut(s) 391, 864
BspQI GCTCTTC 1 cut(s) 284
BspT104I TTCGAA 1 cut(s) 818
BsrI ACTGG 4 cut(s) 271, 409, 730, 920
BssECI CCNNGG 1 cut(s) 447
BssMI GATC 2 cut(s) 383, 856
BssT1I CCWWGG 1 cut(s) 447
Bst4CI ACNGT 2 cut(s) 245, 417
Bst6I CTCTTC 2 cut(s) 94, 284
BstAPI GCANNNNNTGC 1 cut(s) 103
BstBI TTCGAA 1 cut(s) 818
BstC8I GCNNGC 2 cut(s) 108, 296
BstDEI CTNAG 4 cut(s) 339, 493, 531, 665
BstF5I GGATG 2 cut(s) 83, 403
BstKTI GATC 2 cut(s) 386, 859
BstMAI GTCTC 2 cut(s) 540, 634
BstMBI GATC 2 cut(s) 383, 856
BstMWI GCNNNNNNNGC 4 cut(s) 103, 558, 698, 962
BstSFI CTRYAG 2 cut(s) 132, 413
BstV1I GCAGC 3 cut(s) 475, 632, 865
BsuRI GGCC 5 cut(s) 5, 65, 181, 692, 956
BtgZI GCGATG 1 cut(s) 250
BtsCI GGATG 2 cut(s) 83, 403
BtsIMutI CAGTG 2 cut(s) 422, 673
Cac8I GCNNGC 2 cut(s) 108, 296
CaiI CAGNNNCTG 1 cut(s) 878
Cfr13I GGNCC 1 cut(s) 955
CseI GACGC 3 cut(s) 363, 616, 870
Csp6I GTAC 2 cut(s) 171, 425
CviAII CATG 4 cut(s) 67, 80, 694, 804
CviQI GTAC 2 cut(s) 171, 425
DdeI CTNAG 4 cut(s) 339, 493, 531, 665
DpnI GATC 2 cut(s) 385, 858
DpnII GATC 2 cut(s) 383, 856
EaeI YGGCCR 2 cut(s) 3, 63
Eam1104I CTCTTC 2 cut(s) 94, 284
EarI CTCTTC 2 cut(s) 94, 284
Eco130I CCWWGG 1 cut(s) 447
Eco147I AGGCCT 1 cut(s) 181
Eco32I GATATC 1 cut(s) 187
EcoRV GATATC 1 cut(s) 187
EcoT14I CCWWGG 1 cut(s) 447
EcoT22I ATGCAT 1 cut(s) 85
ErhI CCWWGG 1 cut(s) 447
Esp3I CGTCTC 1 cut(s) 634
FaeI CATG 4 cut(s) 70, 83, 697, 807
FatI CATG 4 cut(s) 66, 79, 693, 803
FauI CCCGC 1 cut(s) 671
Fnu4HI GCNGC 3 cut(s) 464, 646, 879
FokI GGATG 2 cut(s) 70, 410
Fsp4HI GCNGC 3 cut(s) 464, 646, 879
FspBI CTAG 3 cut(s) 71, 768, 893
GluI GCNGC 3 cut(s) 464, 646, 879
GsaI CCCAGC 1 cut(s) 658
GsuI CTGGAG 2 cut(s) 713, 903
HaeIII GGCC 5 cut(s) 5, 65, 181, 692, 956
HapII CCGG 1 cut(s) 395
HgaI GACGC 3 cut(s) 363, 616, 870
Hin1II CATG 4 cut(s) 70, 83, 697, 807
HincII GTYRAC 1 cut(s) 367
HindII GTYRAC 1 cut(s) 367
HinfI GANTC 3 cut(s) 128, 815, 888
HpaI GTTAAC 1 cut(s) 367
HpaII CCGG 1 cut(s) 395
HphI GGTGA 2 cut(s) 769, 940
Hpy166II GTNNAC 4 cut(s) 21, 367, 410, 809
Hpy188I TCNGA 2 cut(s) 496, 602
Hpy188III TCNNGA 4 cut(s) 378, 387, 395, 885
Hpy8I GTNNAC 4 cut(s) 21, 367, 410, 809
Hpy99I CGWCG 1 cut(s) 257
HpyAV CCTTC 2 cut(s) 340, 738
HpyCH4III ACNGT 2 cut(s) 245, 417
HpyCH4IV ACGT 2 cut(s) 252, 722
HpyCH4V TGCA 8 cut(s) 83, 110, 176, 463, 707, 741, 794, 965
HpyF10VI GCNNNNNNNGC 4 cut(s) 103, 558, 698, 962
HpyF3I CTNAG 4 cut(s) 339, 493, 531, 665
HpySE526I ACGT 2 cut(s) 252, 722
Hsp92II CATG 4 cut(s) 70, 83, 697, 807
Kpn2I TCCGGA 1 cut(s) 394
KspAI GTTAAC 1 cut(s) 367
Kzo9I GATC 2 cut(s) 383, 856
LguI GCTCTTC 1 cut(s) 284
LmnI GCTCC 2 cut(s) 602, 922
Lsp1109I GCAGC 3 cut(s) 475, 632, 865
LweI GCATC 1 cut(s) 92
MaeI CTAG 3 cut(s) 71, 768, 893
MaeII ACGT 2 cut(s) 252, 722
MaeIII GTNAC 4 cut(s) 245, 281, 723, 928
MalI GATC 2 cut(s) 385, 858
MboI GATC 2 cut(s) 383, 856
MboII GAAGA 4 cut(s) 81, 243, 301, 337
MfeI CAATTG 1 cut(s) 950
MhlI GDGCHC 1 cut(s) 919
MlsI TGGCCA 2 cut(s) 5, 65
MluNI TGGCCA 2 cut(s) 5, 65
MmeI TCCRAC 1 cut(s) 535
Mox20I TGGCCA 2 cut(s) 5, 65
Mph1103I ATGCAT 1 cut(s) 85
MroI TCCGGA 1 cut(s) 394
MscI TGGCCA 2 cut(s) 5, 65
MseI TTAA 1 cut(s) 366
Msp20I TGGCCA 2 cut(s) 5, 65
MspI CCGG 1 cut(s) 395
MunI CAATTG 1 cut(s) 950
Mva1269I GAATGC 2 cut(s) 703, 796
MwoI GCNNNNNNNGC 4 cut(s) 103, 558, 698, 962
NdeII GATC 2 cut(s) 383, 856
NlaIII CATG 4 cut(s) 70, 83, 697, 807
NlaIV GGNNCC 1 cut(s) 351
NmuCI GTSAC 2 cut(s) 723, 928
NsiI ATGCAT 1 cut(s) 85
NspV TTCGAA 1 cut(s) 818
PceI AGGCCT 1 cut(s) 181
PciSI GCTCTTC 1 cut(s) 284
PcsI WCGNNNNNNNCGW 1 cut(s) 249
PctI GAATGC 2 cut(s) 703, 796
PfeI GAWTC 3 cut(s) 128, 815, 888
PkrI GCNGC 3 cut(s) 465, 647, 880
PspFI CCCAGC 1 cut(s) 654
PspN4I GGNNCC 1 cut(s) 351
PspPI GGNCC 1 cut(s) 955
PstNI CAGNNNCTG 1 cut(s) 878
RsaI GTAC 2 cut(s) 172, 426
RsaNI GTAC 2 cut(s) 171, 425
SapI GCTCTTC 1 cut(s) 284
SaqAI TTAA 1 cut(s) 366
SatI GCNGC 3 cut(s) 464, 646, 879
Sau3AI GATC 2 cut(s) 383, 856
Sau96I GGNCC 1 cut(s) 955
SduI GDGCHC 1 cut(s) 919
SetI ASST 9 cut(s) 76, 255, 304, 351, 666, 725, 749, 759, 845
SfaNI GCATC 1 cut(s) 92
SfcI CTRYAG 2 cut(s) 132, 413
SfuI TTCGAA 1 cut(s) 818
SmlI CTYRAG 1 cut(s) 937
SmoI CTYRAG 1 cut(s) 937
SseBI AGGCCT 1 cut(s) 181
SsiI CCGC 1 cut(s) 678
SspI AATATT 1 cut(s) 526
SspMI CTAG 3 cut(s) 71, 768, 893
StuI AGGCCT 1 cut(s) 181
StyI CCWWGG 1 cut(s) 447
TaaI ACNGT 2 cut(s) 245, 417
TaiI ACGT 2 cut(s) 255, 725
TaqI TCGA 1 cut(s) 818
TaqII GACCGA 1 cut(s) 935
TatI WGTACW 1 cut(s) 170
TfiI GAWTC 3 cut(s) 128, 815, 888
Tru1I TTAA 1 cut(s) 366
Tru9I TTAA 1 cut(s) 366
TscAI CASTG 2 cut(s) 422, 673
TseFI GTSAC 2 cut(s) 723, 928
TseI GCWGC 3 cut(s) 463, 645, 878
Tsp45I GTSAC 2 cut(s) 723, 928
TspDTI ATGAA 1 cut(s) 710
TspGWI ACGGA 1 cut(s) 496
TspRI CASTG 2 cut(s) 422, 673
XapI RAATTY 4 cut(s) 474, 611, 797, 820
XspI CTAG 3 cut(s) 71, 768, 893
Zsp2I ATGCAT 1 cut(s) 85
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.