RchiOBHm_Chr6g0279521

GDSL esterase lipase At5g55050-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0000448
Physical Location & Seq
Reverse (-)
42638460 .. 42639347
888 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ25066

Sequence Viewer

Length: 306 bp
ATGGCCAAAATGGGTGCAGCAGCAACTGAGAAGTTTCTTTCGAAGTCTTTGATCTTCACCAGCAGTGGAAGCAATGACCTTTTCGGATATTATCATTCAAACAGTTCAATTCCCAAAGAAGAATTCTTGTCATCTTTAGGACTTGCTTATGAGAACCACTTGAAGCTGACATATTTATGTTATAGCACACTGTCTAATTTTACACAAGTGGAAGATGCATGTTGTGGAGCTGGGAAGCTCGGTGGTGAATCCTTCTGTACACCAGATGATAATCTTTGTTCGAACCGCGACCAATACTTGTTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

101

Amino Acids

11.07

Weight (kDa)

4.92

Isoelectric Point (pI)

28.13

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000448)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g10790 FvH4_1g10790 FvH4_1g10790 FvH4_1g10791 FvH4_1g10800 FvH4_2g15970 FvH4_2g15980 FvH4_2g15990
malus_domestica MD02G1121900.v1.1 MD05G1128700.v1.1 MD10G1131900.v1.1 MD15G1235500.v1.1 MD15G1235600.v1.1
prunus_persica Prupe.7G177500_v2.0.a1 Prupe.7G177600_v2.0.a1 Prupe.8G172800_v2.0.a1
pyrus_communis pycom05g12460 pycom05g12470 pycom10g11300
rosa_chinensis RchiOBHm_Chr2g0097971 RchiOBHm_Chr2g0098021 RchiOBHm_Chr2g0098061 RchiOBHm_Chr6g0257301 RchiOBHm_Chr6g0279511 RchiOBHm_Chr6g0279521
rosa_laevigata RLG00000013139 RLG00000013140 RLG00000013141 RLG00000016747 RLG00000016749 RLG00000016750 RLG00000016751 RLG00000016752
rosa_multiflora Rmu_sc0002648.1_g000005 Rmu_sc0002648.1_g000006 Rmu_sc0004649.1_g000019 Rmu_sc0004649.1_g000021 Rmu_sc0009572.1_g000002 Rmu_sc0013919.1_g000008 Rmu_sc0013919.1_g000009
rosa_roxburghii Rroxscaffold_2G00144370 Rroxscaffold_2G00144380 Rroxscaffold_2G00144390 Rroxscaffold_2G00144410 Rroxscaffold_4G00294600 Rroxscaffold_7G00188840 Rroxscaffold_7G00188850 Rroxscaffold_7G00188860
rosa_rugosa Rorug02G0069400 Rorug02G0069600 Rorug02G0069700 Rorug02G0069800 Rorug06G0098000.1 Rorug06G0125000 Rorug06G0125100 Rorug06G0125200
rosa_samantha Rh2AG116400 Rh2AG116800 Rh2AG116900 Rh2AG117000 Rh2BG119300 Rh2BG119500 Rh2BG119600 Rh2BG119700 Rh2BG119800 Rh2CG120900 Rh2CG121000 Rh2CG121200 Rh2CG121300 Rh2CG121400 Rh2CG121500 Rh2DG120600 Rh2DG121100 Rh2DG121200 Rh2DG121500 Rh6AG091200 Rh6AG234100 Rh6BG238400 Rh6BG238500 Rh6BG238600 Rh6CG078800 Rh6CG240400 Rh6CG240500 Rh6CG240600 Rh6DG074600 Rh6DG232000 Rh6DG232100
rosa_wichuraiana Rw2G009120 Rw2G009130 Rw2G009140 Rw6G020420 Rw6G020430 Rw6G020440

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 288
AciI CCGC 1 cut(s) 286
AcoI YGGCCR 1 cut(s) 3
AcsI RAATTY 1 cut(s) 122
AfaI GTAC 1 cut(s) 259
AgsI TTSAA 3 cut(s) 99, 108, 163
AjuI GAANNNNNNNTTGG 2 cut(s) 107, 139
AluBI AGCT 3 cut(s) 166, 230, 238
AluI AGCT 3 cut(s) 166, 230, 238
AlwNI CAGNNNCTG 1 cut(s) 26
AoxI GGCC 1 cut(s) 3
ApeKI GCWGC 2 cut(s) 17, 20
ApoI RAATTY 1 cut(s) 122
AsuHPI GGTGA 2 cut(s) 49, 257
AsuII TTCGAA 2 cut(s) 41, 281
BalI TGGCCA 1 cut(s) 5
BbvI GCAGC 2 cut(s) 29, 32
BfaI CTAG 1 cut(s) 304
BisI GCNGC 2 cut(s) 18, 21
BlsI GCNGC 2 cut(s) 19, 22
BmsI GCATC 1 cut(s) 205
Bpu14I TTCGAA 2 cut(s) 41, 281
BsaBI GATNNNNATC 1 cut(s) 270
Bse3DI GCAATG 1 cut(s) 79
Bse8I GATNNNNATC 1 cut(s) 270
BseJI GATNNNNATC 1 cut(s) 270
BseMI GCAATG 1 cut(s) 79
BseMII CTCAG 1 cut(s) 18
BseXI GCAGC 2 cut(s) 29, 32
BseYI CCCAGC 1 cut(s) 230
BsgI GTGCAG 1 cut(s) 36
Bsh1236I CGCG 1 cut(s) 288
BshFI GGCC 1 cut(s) 5
BsnI GGCC 1 cut(s) 5
Bsp119I TTCGAA 2 cut(s) 41, 281
Bsp1407I TGTACA 1 cut(s) 257
Bsp143I GATC 1 cut(s) 51
BspACI CCGC 1 cut(s) 286
BspANI GGCC 1 cut(s) 5
BspCNI CTCAG 1 cut(s) 19
BspFNI CGCG 1 cut(s) 288
BspT104I TTCGAA 2 cut(s) 41, 281
BsrDI GCAATG 1 cut(s) 79
BsrGI TGTACA 1 cut(s) 257
BssMI GATC 1 cut(s) 51
Bst4CI ACNGT 2 cut(s) 104, 192
BstAUI TGTACA 1 cut(s) 257
BstBI TTCGAA 2 cut(s) 41, 281
BstDEI CTNAG 1 cut(s) 27
BstFNI CGCG 1 cut(s) 288
BstKTI GATC 1 cut(s) 54
BstMBI GATC 1 cut(s) 51
BstMWI GCNNNNNNNGC 1 cut(s) 69
BstNSI RCATGY 1 cut(s) 222
BstUI CGCG 1 cut(s) 288
BstV1I GCAGC 2 cut(s) 29, 32
BsuRI GGCC 1 cut(s) 5
BtsI GCAGTG 1 cut(s) 70
BtsIMutI CAGTG 2 cut(s) 70, 188
CaiI CAGNNNCTG 1 cut(s) 26
Csp6I GTAC 1 cut(s) 258
CviAII CATG 1 cut(s) 219
CviJI RGCY 4 cut(s) 5, 166, 230, 238
CviKI_1 RGCY 4 cut(s) 5, 166, 230, 238
CviQI GTAC 1 cut(s) 258
DdeI CTNAG 1 cut(s) 27
DpnI GATC 1 cut(s) 53
DpnII GATC 1 cut(s) 51
EaeI YGGCCR 1 cut(s) 3
EcoRI GAATTC 1 cut(s) 122
EcoT22I ATGCAT 1 cut(s) 220
FaeI CATG 1 cut(s) 222
FaiI YATR 5 cut(s) 150, 172, 178, 183, 220
FatI CATG 1 cut(s) 218
Fnu4HI GCNGC 2 cut(s) 18, 21
Fsp4HI GCNGC 2 cut(s) 18, 21
FspBI CTAG 1 cut(s) 304
GluI GCNGC 2 cut(s) 18, 21
GsaI CCCAGC 1 cut(s) 234
HaeIII GGCC 1 cut(s) 5
Hin1II CATG 1 cut(s) 222
HinfI GANTC 1 cut(s) 248
HphI GGTGA 2 cut(s) 49, 257
Hpy166II GTNNAC 1 cut(s) 260
Hpy188I TCNGA 1 cut(s) 86
Hpy8I GTNNAC 1 cut(s) 260
HpyAV CCTTC 1 cut(s) 262
HpyCH4III ACNGT 2 cut(s) 104, 192
HpyCH4V TGCA 2 cut(s) 17, 218
HpyF10VI GCNNNNNNNGC 1 cut(s) 69
HpyF3I CTNAG 1 cut(s) 27
Hsp92II CATG 1 cut(s) 222
Kzo9I GATC 1 cut(s) 51
LmnI GCTCC 1 cut(s) 227
LpnPI CCDG 3 cut(s) 73, 216, 276
Lsp1109I GCAGC 2 cut(s) 29, 32
LweI GCATC 1 cut(s) 205
MaeI CTAG 1 cut(s) 304
MalI GATC 1 cut(s) 53
MboI GATC 1 cut(s) 51
MboII GAAGA 3 cut(s) 46, 131, 224
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 3 cut(s) 108, 122, 196
MluNI TGGCCA 1 cut(s) 5
Mox20I TGGCCA 1 cut(s) 5
Mph1103I ATGCAT 1 cut(s) 220
MscI TGGCCA 1 cut(s) 5
MslI CAYNNNNRTG 1 cut(s) 175
Msp20I TGGCCA 1 cut(s) 5
MvnI CGCG 1 cut(s) 288
MwoI GCNNNNNNNGC 1 cut(s) 69
NdeII GATC 1 cut(s) 51
NlaIII CATG 1 cut(s) 222
NsiI ATGCAT 1 cut(s) 220
NspI RCATGY 1 cut(s) 222
NspV TTCGAA 2 cut(s) 41, 281
PfeI GAWTC 1 cut(s) 248
PkrI GCNGC 2 cut(s) 19, 22
PspFI CCCAGC 1 cut(s) 230
PstNI CAGNNNCTG 1 cut(s) 26
RsaI GTAC 1 cut(s) 259
RsaNI GTAC 1 cut(s) 258
RseI CAYNNNNRTG 1 cut(s) 175
SatI GCNGC 2 cut(s) 18, 21
Sau3AI GATC 1 cut(s) 51
SetI ASST 4 cut(s) 81, 168, 232, 240
SfaNI GCATC 1 cut(s) 205
SfuI TTCGAA 2 cut(s) 41, 281
SmiMI CAYNNNNRTG 1 cut(s) 175
Sse9I AATT 3 cut(s) 108, 122, 196
SsiI CCGC 1 cut(s) 286
SspMI CTAG 1 cut(s) 304
TaaI ACNGT 2 cut(s) 104, 192
TaqI TCGA 2 cut(s) 41, 281
TasI AATT 3 cut(s) 108, 122, 196
TatI WGTACW 1 cut(s) 257
TfiI GAWTC 1 cut(s) 248
TscAI CASTG 2 cut(s) 70, 195
TseI GCWGC 2 cut(s) 17, 20
TspRI CASTG 2 cut(s) 70, 195
XapI RAATTY 1 cut(s) 122
XceI RCATGY 1 cut(s) 222
XspI CTAG 1 cut(s) 304
Zsp2I ATGCAT 1 cut(s) 220
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.