Rmu_sc0004649.1_g000019

GDSL esterase lipase At5g55050-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0004649.1
OG0000448
Physical Location & Seq
Forward (+)
60929 .. 62626
1698 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0004649.1_g000019.1.cds

Sequence Viewer

Length: 789 bp
atggctgttcttagttcagcagcagcagctaagccagtactattgccaccaattttcatactaggtgattcaactgctgatataggaaccaataacttcttgccaggtagcaaggcaagggcagactttccccacaatggcattgactttcctaactcaccaagacccactggaaggttcagcaatggcctcaacagtgctgattttctagcccagcattttggttacaagagaagtccaaggccctttctttctctcagtgctacttctcttcaaaagaagaagttcagaggtattaatttgcttctggagggtctgactttatacactcttggagcaaggaagtttggaatcattagcgtcgccccgattggctgctgcccgtctcaaaggatttttaatgctactggggggtgtttggaggagttgaatgatcatgcaatagccttccatttaaagctggatgccctattgtgcaagctcagcaccgaacacgaaggctttaagtactcccttggaaattcatatgaaatgacaataaatgtcgtacagaaccctcctccattcaattttacacaagtggaagctgcatgttgtggagctgggaagctcggtggtgaatccttctgtacaccagatgctaatctttgtttgaaccgcgaccaatacttgttctgggatctattccatccaacaggggcggcttctaagttggcagctgtgaccctctactatggaggaccacaatttgtatccccaattaacttcgctcaattacccgaggcttaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

262

Amino Acids

28.22

Weight (kDa)

8.1

Isoelectric Point (pI)

28.75

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000448)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g10790 FvH4_1g10790 FvH4_1g10790 FvH4_1g10791 FvH4_1g10800 FvH4_2g15970 FvH4_2g15980 FvH4_2g15990
malus_domestica MD02G1121900.v1.1 MD05G1128700.v1.1 MD10G1131900.v1.1 MD15G1235500.v1.1 MD15G1235600.v1.1
prunus_persica Prupe.7G177500_v2.0.a1 Prupe.7G177600_v2.0.a1 Prupe.8G172800_v2.0.a1
pyrus_communis pycom05g12460 pycom05g12470 pycom10g11300
rosa_chinensis RchiOBHm_Chr2g0097971 RchiOBHm_Chr2g0098021 RchiOBHm_Chr2g0098061 RchiOBHm_Chr6g0257301 RchiOBHm_Chr6g0279511 RchiOBHm_Chr6g0279521
rosa_laevigata RLG00000013139 RLG00000013140 RLG00000013141 RLG00000016747 RLG00000016749 RLG00000016750 RLG00000016751 RLG00000016752
rosa_multiflora Rmu_sc0002648.1_g000005 Rmu_sc0002648.1_g000006 Rmu_sc0004649.1_g000019 Rmu_sc0004649.1_g000021 Rmu_sc0009572.1_g000002 Rmu_sc0013919.1_g000008 Rmu_sc0013919.1_g000009
rosa_roxburghii Rroxscaffold_2G00144370 Rroxscaffold_2G00144380 Rroxscaffold_2G00144390 Rroxscaffold_2G00144410 Rroxscaffold_4G00294600 Rroxscaffold_7G00188840 Rroxscaffold_7G00188850 Rroxscaffold_7G00188860
rosa_rugosa Rorug02G0069400 Rorug02G0069600 Rorug02G0069700 Rorug02G0069800 Rorug06G0098000.1 Rorug06G0125000 Rorug06G0125100 Rorug06G0125200
rosa_samantha Rh2AG116400 Rh2AG116800 Rh2AG116900 Rh2AG117000 Rh2BG119300 Rh2BG119500 Rh2BG119600 Rh2BG119700 Rh2BG119800 Rh2CG120900 Rh2CG121000 Rh2CG121200 Rh2CG121300 Rh2CG121400 Rh2CG121500 Rh2DG120600 Rh2DG121100 Rh2DG121200 Rh2DG121500 Rh6AG091200 Rh6AG234100 Rh6BG238400 Rh6BG238500 Rh6BG238600 Rh6CG078800 Rh6CG240400 Rh6CG240500 Rh6CG240600 Rh6DG074600 Rh6DG232000 Rh6DG232100
rosa_wichuraiana Rw2G009120 Rw2G009130 Rw2G009140 Rw6G020420 Rw6G020430 Rw6G020440

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 660
AciI CCGC 2 cut(s) 658, 701
AclWI GGATC 1 cut(s) 687
AcsI RAATTY 1 cut(s) 520
AfaI GTAC 4 cut(s) 39, 509, 549, 631
AfiI CCNNNNNNNGG 2 cut(s) 137, 174
AgsI TTSAA 5 cut(s) 72, 275, 430, 568, 655
AjnI CCWGG 1 cut(s) 103
AluBI AGCT 7 cut(s) 29, 460, 481, 587, 602, 610, 719
AluI AGCT 7 cut(s) 29, 460, 481, 587, 602, 610, 719
Alw26I GTCTC 1 cut(s) 390
AlwI GGATC 1 cut(s) 687
Ama87I CYCGRG 1 cut(s) 779
AoxI GGCC 2 cut(s) 187, 242
ApeKI GCWGC 7 cut(s) 20, 23, 26, 375, 378, 587, 716
ApoI RAATTY 1 cut(s) 520
AseI ATTAAT 1 cut(s) 297
Asp700I GAANNNNTTC 1 cut(s) 284
AspS9I GGNCC 2 cut(s) 243, 740
AsuHPI GGTGA 3 cut(s) 77, 150, 629
AvaI CYCGRG 1 cut(s) 779
AvaII GGWCC 1 cut(s) 740
BbvI GCAGC 7 cut(s) 32, 35, 38, 362, 365, 574, 728
BccI CCATC 1 cut(s) 696
BciT130I CCWGG 1 cut(s) 105
BciVI GTATCC 1 cut(s) 763
BclI TGATCA 1 cut(s) 433
BcoDI GTCTC 1 cut(s) 390
BfaI CTAG 2 cut(s) 62, 209
BfuI GTATCC 1 cut(s) 763
BisI GCNGC 8 cut(s) 21, 24, 27, 376, 379, 588, 702, 717
BlpI GCTNAGC 2 cut(s) 30, 482
BlsI GCNGC 8 cut(s) 22, 25, 28, 377, 380, 589, 703, 718
BmcAI AGTACT 2 cut(s) 39, 509
Bme1390I CCNGG 1 cut(s) 105
Bme18I GGWCC 1 cut(s) 740
BmeT110I CYCGRG 1 cut(s) 779
BmgT120I GGNCC 2 cut(s) 243, 740
BmiI GGNNCC 1 cut(s) 88
BmrFI CCNGG 1 cut(s) 105
BmrI ACTGGG 1 cut(s) 417
BmsI GCATC 2 cut(s) 454, 628
BmuI ACTGGG 1 cut(s) 417
BpmI CTGGAG 1 cut(s) 329
Bpu1102I GCTNAGC 2 cut(s) 30, 482
BsaBI GATNNNNATC 1 cut(s) 642
BsaJI CCNNGG 3 cut(s) 239, 514, 780
Bsc4I CCNNNNNNNGG 2 cut(s) 137, 174
Bse1I ACTGG 3 cut(s) 35, 175, 412
Bse3DI GCAATG 1 cut(s) 190
Bse8I GATNNNNATC 1 cut(s) 642
BseBI CCWGG 1 cut(s) 105
BseDI CCNNGG 3 cut(s) 239, 514, 780
BseGI GGATG 2 cut(s) 469, 688
BseJI GATNNNNATC 1 cut(s) 642
BseLI CCNNNNNNNGG 2 cut(s) 137, 174
BseMI GCAATG 1 cut(s) 190
BseMII CTCAG 2 cut(s) 271, 496
BseNI ACTGG 3 cut(s) 35, 175, 412
BseRI GAGGAG 2 cut(s) 437, 549
BseXI GCAGC 7 cut(s) 32, 35, 38, 362, 365, 574, 728
BseYI CCCAGC 2 cut(s) 213, 602
Bsh1236I CGCG 1 cut(s) 660
BshFI GGCC 2 cut(s) 189, 244
BsiHKCI CYCGRG 1 cut(s) 779
BslI CCNNNNNNNGG 2 cut(s) 137, 174
BsmAI GTCTC 1 cut(s) 390
BsmBI CGTCTC 1 cut(s) 390
BsnI GGCC 2 cut(s) 189, 244
BsoBI CYCGRG 1 cut(s) 779
Bsp1407I TGTACA 1 cut(s) 629
Bsp143I GATC 2 cut(s) 433, 679
Bsp1720I GCTNAGC 2 cut(s) 30, 482
BspACI CCGC 2 cut(s) 658, 701
BspANI GGCC 2 cut(s) 189, 244
BspCNI CTCAG 2 cut(s) 270, 495
BspFNI CGCG 1 cut(s) 660
BspLI GGNNCC 1 cut(s) 88
BspPI GGATC 1 cut(s) 687
BsrDI GCAATG 1 cut(s) 190
BsrGI TGTACA 1 cut(s) 629
BsrI ACTGG 3 cut(s) 35, 175, 412
BssECI CCNNGG 3 cut(s) 239, 514, 780
BssMI GATC 2 cut(s) 433, 679
BssT1I CCWWGG 2 cut(s) 239, 514
Bst2UI CCWGG 1 cut(s) 105
Bst4CI ACNGT 1 cut(s) 197
Bst6I CTCTTC 1 cut(s) 276
BstAUI TGTACA 1 cut(s) 629
BstC8I GCNNGC 1 cut(s) 479
BstDEI CTNAG 5 cut(s) 11, 30, 257, 482, 708
BstF5I GGATG 2 cut(s) 469, 688
BstFNI CGCG 1 cut(s) 660
BstKTI GATC 2 cut(s) 436, 682
BstMAI GTCTC 1 cut(s) 390
BstMBI GATC 2 cut(s) 433, 679
BstMWI GCNNNNNNNGC 2 cut(s) 26, 483
BstNI CCWGG 1 cut(s) 105
BstNSI RCATGY 1 cut(s) 594
BstSCI CCNGG 1 cut(s) 103
BstUI CGCG 1 cut(s) 660
BstV1I GCAGC 7 cut(s) 32, 35, 38, 362, 365, 574, 728
BstX2I RGATCY 1 cut(s) 679
BstXI CCANNNNNNTGG 1 cut(s) 221
BstYI RGATCY 1 cut(s) 679
BsuI GTATCC 1 cut(s) 763
BsuRI GGCC 2 cut(s) 189, 244
BtsCI GGATG 2 cut(s) 469, 688
BtsIMutI CAGTG 3 cut(s) 168, 202, 265
Cac8I GCNNGC 1 cut(s) 479
Cfr13I GGNCC 2 cut(s) 243, 740
CseI GACGC 1 cut(s) 349
Csp6I GTAC 4 cut(s) 38, 508, 548, 630
CviAII CATG 2 cut(s) 437, 591
CviQI GTAC 4 cut(s) 38, 508, 548, 630
DdeI CTNAG 5 cut(s) 11, 30, 257, 482, 708
DpnI GATC 2 cut(s) 435, 681
DpnII GATC 2 cut(s) 433, 679
DraI TTTAAA 1 cut(s) 456
Eam1104I CTCTTC 1 cut(s) 276
EarI CTCTTC 1 cut(s) 276
Eco130I CCWWGG 2 cut(s) 239, 514
Eco47I GGWCC 1 cut(s) 740
Eco88I CYCGRG 1 cut(s) 779
EcoO109I RGGNCCY 1 cut(s) 243
EcoRII CCWGG 1 cut(s) 103
EcoT14I CCWWGG 2 cut(s) 239, 514
ErhI CCWWGG 2 cut(s) 239, 514
Esp3I CGTCTC 1 cut(s) 390
FaeI CATG 2 cut(s) 440, 594
FaiI YATR 8 cut(s) 59, 83, 325, 438, 526, 528, 592, 735
FatI CATG 2 cut(s) 436, 590
FauNDI CATATG 1 cut(s) 526
FbaI TGATCA 1 cut(s) 433
Fnu4HI GCNGC 8 cut(s) 21, 24, 27, 376, 379, 588, 702, 717
FokI GGATG 2 cut(s) 476, 675
Fsp4HI GCNGC 8 cut(s) 21, 24, 27, 376, 379, 588, 702, 717
FspBI CTAG 2 cut(s) 62, 209
GluI GCNGC 8 cut(s) 21, 24, 27, 376, 379, 588, 702, 717
GsaI CCCAGC 2 cut(s) 217, 606
GsuI CTGGAG 1 cut(s) 329
HaeIII GGCC 2 cut(s) 189, 244
HgaI GACGC 1 cut(s) 349
Hin1II CATG 2 cut(s) 440, 594
HinfI GANTC 3 cut(s) 68, 351, 620
HphI GGTGA 3 cut(s) 77, 150, 629
Hpy166II GTNNAC 1 cut(s) 632
Hpy188I TCNGA 2 cut(s) 290, 318
Hpy188III TCNNGA 1 cut(s) 308
Hpy8I GTNNAC 1 cut(s) 632
Hpy99I CGWCG 1 cut(s) 365
HpyAV CCTTC 4 cut(s) 168, 457, 491, 634
HpyCH4III ACNGT 1 cut(s) 197
HpyCH4V TGCA 3 cut(s) 440, 477, 590
HpyF10VI GCNNNNNNNGC 2 cut(s) 26, 483
HpyF3I CTNAG 5 cut(s) 11, 30, 257, 482, 708
Hsp92II CATG 2 cut(s) 440, 594
Ksp22I TGATCA 1 cut(s) 433
Kzo9I GATC 2 cut(s) 433, 679
LmnI GCTCC 2 cut(s) 335, 599
Lsp1109I GCAGC 7 cut(s) 32, 35, 38, 362, 365, 574, 728
LweI GCATC 2 cut(s) 454, 628
MaeI CTAG 2 cut(s) 62, 209
MaeIII GTNAC 2 cut(s) 224, 721
MalI GATC 2 cut(s) 435, 681
MboI GATC 2 cut(s) 433, 679
MboII GAAGA 2 cut(s) 263, 292
MflI RGATCY 1 cut(s) 679
MluCI AATT 7 cut(s) 51, 298, 520, 568, 746, 759, 773
MmeI TCCRAC 1 cut(s) 716
MnlI CCTC 9 cut(s) 200, 284, 304, 415, 567, 570, 731, 737, 775
MroXI GAANNNNTTC 1 cut(s) 284
MseI TTAA 6 cut(s) 297, 399, 455, 504, 762, 787
MspA1I CMGCKG 1 cut(s) 719
MspR9I CCNGG 1 cut(s) 105
MvaI CCWGG 1 cut(s) 105
MvnI CGCG 1 cut(s) 660
MwoI GCNNNNNNNGC 2 cut(s) 26, 483
NdeI CATATG 1 cut(s) 526
NdeII GATC 2 cut(s) 433, 679
NlaIII CATG 2 cut(s) 440, 594
NlaIV GGNNCC 1 cut(s) 88
NmuCI GTSAC 1 cut(s) 721
NspI RCATGY 1 cut(s) 594
PdmI GAANNNNTTC 1 cut(s) 284
PfeI GAWTC 3 cut(s) 68, 351, 620
PkrI GCNGC 8 cut(s) 22, 25, 28, 377, 380, 589, 703, 718
PshBI ATTAAT 1 cut(s) 297
Psp6I CCWGG 1 cut(s) 103
PspFI CCCAGC 2 cut(s) 213, 602
PspGI CCWGG 1 cut(s) 103
PspN4I GGNNCC 1 cut(s) 88
PspPI GGNCC 2 cut(s) 243, 740
PsuI RGATCY 1 cut(s) 679
PvuII CAGCTG 1 cut(s) 719
RsaI GTAC 4 cut(s) 39, 509, 549, 631
RsaNI GTAC 4 cut(s) 38, 508, 548, 630
SaqAI TTAA 6 cut(s) 297, 399, 455, 504, 762, 787
SatI GCNGC 8 cut(s) 21, 24, 27, 376, 379, 588, 702, 717
Sau3AI GATC 2 cut(s) 433, 679
Sau96I GGNCC 2 cut(s) 243, 740
ScaI AGTACT 2 cut(s) 39, 509
ScrFI CCNGG 1 cut(s) 105
SfaNI GCATC 2 cut(s) 454, 628
SinI GGWCC 1 cut(s) 740
Sse9I AATT 7 cut(s) 51, 298, 520, 568, 746, 759, 773
SsiI CCGC 2 cut(s) 658, 701
SspMI CTAG 2 cut(s) 62, 209
StyD4I CCNGG 1 cut(s) 103
StyI CCWWGG 2 cut(s) 239, 514
TaaI ACNGT 1 cut(s) 197
TasI AATT 7 cut(s) 51, 298, 520, 568, 746, 759, 773
TatI WGTACW 3 cut(s) 37, 507, 629
TauI GCSGC 1 cut(s) 704
TfiI GAWTC 3 cut(s) 68, 351, 620
Tru1I TTAA 6 cut(s) 297, 399, 455, 504, 762, 787
Tru9I TTAA 6 cut(s) 297, 399, 455, 504, 762, 787
TscAI CASTG 3 cut(s) 175, 202, 265
TseFI GTSAC 1 cut(s) 721
TseI GCWGC 7 cut(s) 20, 23, 26, 375, 378, 587, 716
Tsp45I GTSAC 1 cut(s) 721
TspDTI ATGAA 3 cut(s) 46, 513, 543
TspRI CASTG 3 cut(s) 175, 202, 265
VpaK11BI GGWCC 1 cut(s) 740
VspI ATTAAT 1 cut(s) 297
XapI RAATTY 1 cut(s) 520
XceI RCATGY 1 cut(s) 594
XmnI GAANNNNTTC 1 cut(s) 284
XspI CTAG 2 cut(s) 62, 209
ZrmI AGTACT 2 cut(s) 39, 509
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.