Rh2DG121200

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0000448
Physical Location & Seq
Forward (+)
10091859 .. 10095027
3169 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG121200.1

Sequence Viewer

Length: 1110 bp
ATGGCCAAGTCTGCAATGTTTTCTCTTTGGCTCAATATTATTGTGTATCTCGGCATACTTGGATTGCAAGTCTCACACTCTCAGCCATCTGTGCCAGCACTTTACATATTTGGTGACTCCACAGCTGATGTTGGAACCAACAATTATTTGCCCCGTTCTAGGGCGCGGGCCGATTTCCCTTATAACGGGATTGATTTTCCTCAATCTAGACCAACTGGGAGATTTAGCAACGGTTTCAACACTATTGATTATCTAGCCCAGTATCTGAAATTCTATGAAAGCCCGCCGCCTTTTCTCTCTTTTTCGGACAGCAATATTGCGAAATTTCTCATCAAGACGAAGGGAATCAATTTTGCTTCAGGAGGGTCAGGACTTCTGTATTCTACAGGACAAAAGAAGTATAGAAGGGTGATTTCTTTTGGAGAACAAGTCCAACAATTTGCAACTGTTCGCAGCCATATTTCAGAATTATTGGGTGCGAGTGCAGTCGCTAATATCTCCAAGTCTCTCTTCATCATCAGCGTCGGAAGCAATGACATATTCGAACATTTTGATTATAACTACACTAGTAACCCTATTGTTATTGGGCAAAACTACATCGCCACTCTCATCTCCACCTACGAAACTCATCTAAGGAATTTATATGAGCTAGGAGCCAGAAGATTTGGGATTATTAGTGTTGGGGCAATTGGGTGCTGTCCGTGTCAACGTAATTCGAGTAGATCTGGTGATTGTTTCGAGGCCATGAATGTGGATGCTCAGTTGTTCTACACTGCACTCCTACGTCTCTTGCAAAAGTTGAGTTCAGAGTGTGAGGGGTTGATGTATGCACTTGGAGATTCATACAAAATGACAAAATATATTATCGACAACCCCGATCAGTCTGTTTTTACCGAGATGAAAAAAGCTTGCTGTGGAAAAGGACAGTTGAAAGCAAAGCACGCATGTAAACAGGATTCGGATCTGTGTGAAAATCGCACAACCCACTTGTTTTGGGACCGGTACCATCCAACACAGACCGCTTCTAATCTCGCAGCCTTTGGTCTTTATAATGGTGACAAATCATCCGTGGTGCCCATGAGTTTTGCTGAATTGGCCCTTCGGCCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

369

Amino Acids

41.01

Weight (kDa)

8.72

Isoelectric Point (pI)

44.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 35 - 346 4.8e-28 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000448)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g10790 FvH4_1g10790 FvH4_1g10790 FvH4_1g10791 FvH4_1g10800 FvH4_2g15970 FvH4_2g15980 FvH4_2g15990
malus_domestica MD02G1121900.v1.1 MD05G1128700.v1.1 MD10G1131900.v1.1 MD15G1235500.v1.1 MD15G1235600.v1.1
prunus_persica Prupe.7G177500_v2.0.a1 Prupe.7G177600_v2.0.a1 Prupe.8G172800_v2.0.a1
pyrus_communis pycom05g12460 pycom05g12470 pycom10g11300
rosa_chinensis RchiOBHm_Chr2g0097971 RchiOBHm_Chr2g0098021 RchiOBHm_Chr2g0098061 RchiOBHm_Chr6g0257301 RchiOBHm_Chr6g0279511 RchiOBHm_Chr6g0279521
rosa_laevigata RLG00000013139 RLG00000013140 RLG00000013141 RLG00000016747 RLG00000016749 RLG00000016750 RLG00000016751 RLG00000016752
rosa_multiflora Rmu_sc0002648.1_g000005 Rmu_sc0002648.1_g000006 Rmu_sc0004649.1_g000019 Rmu_sc0004649.1_g000021 Rmu_sc0009572.1_g000002 Rmu_sc0013919.1_g000008 Rmu_sc0013919.1_g000009
rosa_roxburghii Rroxscaffold_2G00144370 Rroxscaffold_2G00144380 Rroxscaffold_2G00144390 Rroxscaffold_2G00144410 Rroxscaffold_4G00294600 Rroxscaffold_7G00188840 Rroxscaffold_7G00188850 Rroxscaffold_7G00188860
rosa_rugosa Rorug02G0069400 Rorug02G0069600 Rorug02G0069700 Rorug02G0069800 Rorug06G0098000.1 Rorug06G0125000 Rorug06G0125100 Rorug06G0125200
rosa_samantha Rh2AG116400 Rh2AG116800 Rh2AG116900 Rh2AG117000 Rh2BG119300 Rh2BG119500 Rh2BG119600 Rh2BG119700 Rh2BG119800 Rh2CG120900 Rh2CG121000 Rh2CG121200 Rh2CG121300 Rh2CG121400 Rh2CG121500 Rh2DG120600 Rh2DG121100 Rh2DG121200 Rh2DG121500 Rh6AG091200 Rh6AG234100 Rh6BG238400 Rh6BG238500 Rh6BG238600 Rh6CG078800 Rh6CG240400 Rh6CG240500 Rh6CG240600 Rh6DG074600 Rh6DG232000 Rh6DG232100
rosa_wichuraiana Rw2G009120 Rw2G009130 Rw2G009140 Rw6G020420 Rw6G020430 Rw6G020440

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 183, 558, 1050
Acc65I GGTACC 1 cut(s) 1002
AccB1I GGYRCC 2 cut(s) 1002, 1072
AccII CGCG 1 cut(s) 166
AciI CCGC 4 cut(s) 166, 284, 287, 1020
AclWI GGATC 1 cut(s) 969
AcoI YGGCCR 2 cut(s) 3, 1103
AcsI RAATTY 3 cut(s) 269, 323, 637
AcuI CTGAAG 1 cut(s) 342
AfaI GTAC 1 cut(s) 1004
AfiI CCNNNNNNNGG 3 cut(s) 159, 160, 185
AgeI ACCGGT 1 cut(s) 999
AgsI TTSAA 2 cut(s) 238, 931
AhlI ACTAGT 1 cut(s) 566
AjuI GAANNNNNNNTTGG 2 cut(s) 494, 526
AluBI AGCT 3 cut(s) 125, 649, 908
AluI AGCT 3 cut(s) 125, 649, 908
Alw26I GTCTC 3 cut(s) 76, 510, 791
AlwI GGATC 1 cut(s) 969
AlwNI CAGNNNCTG 1 cut(s) 265
AoxI GGCC 5 cut(s) 3, 168, 741, 1095, 1103
ApeKI GCWGC 2 cut(s) 453, 1034
ApoI RAATTY 3 cut(s) 269, 323, 637
AsiGI ACCGGT 1 cut(s) 999
Asp718I GGTACC 1 cut(s) 1002
AspLEI GCGC 1 cut(s) 166
AspS9I GGNCC 3 cut(s) 168, 997, 1096
AsuHPI GGTGA 4 cut(s) 125, 421, 740, 1067
AsuII TTCGAA 1 cut(s) 543
AvaII GGWCC 1 cut(s) 997
BaeGI GKGCMC 1 cut(s) 1077
BalI TGGCCA 1 cut(s) 5
BanI GGYRCC 2 cut(s) 1002, 1072
BbvI GCAGC 2 cut(s) 465, 1046
BccI CCATC 2 cut(s) 94, 1014
BcoDI GTCTC 3 cut(s) 76, 510, 791
BcuI ACTAGT 1 cut(s) 566
BfaI CTAG 5 cut(s) 159, 207, 254, 567, 650
BfmI CTRYAG 1 cut(s) 384
BglII AGATCT 1 cut(s) 722
BisI GCNGC 3 cut(s) 287, 454, 1035
BlsI GCNGC 3 cut(s) 288, 455, 1036
Bme18I GGWCC 1 cut(s) 997
BmgT120I GGNCC 3 cut(s) 168, 997, 1096
BmiI GGNNCC 5 cut(s) 136, 655, 998, 1004, 1074
BmrI ACTGGG 2 cut(s) 225, 253
BmsI GCATC 1 cut(s) 745
BmuI ACTGGG 2 cut(s) 225, 253
Bpu14I TTCGAA 1 cut(s) 543
BsaBI GATNNNNATC 1 cut(s) 960
BsaJI CCNNGG 1 cut(s) 1068
BsaWI WCCGGW 1 cut(s) 999
BsaXI ACNNNNNCTCC 2 cut(s) 414, 444
Bsc4I CCNNNNNNNGG 3 cut(s) 159, 160, 185
Bse118I RCCGGY 1 cut(s) 999
Bse1I ACTGG 2 cut(s) 220, 259
Bse3DI GCAATG 2 cut(s) 21, 538
Bse8I GATNNNNATC 1 cut(s) 960
BseDI CCNNGG 1 cut(s) 1068
BseGI GGATG 3 cut(s) 760, 1006, 1064
BseJI GATNNNNATC 1 cut(s) 960
BseLI CCNNNNNNNGG 3 cut(s) 159, 160, 185
BseMI GCAATG 2 cut(s) 21, 538
BseMII CTCAG 2 cut(s) 95, 773
BseNI ACTGG 2 cut(s) 220, 259
BseSI GKGCMC 1 cut(s) 1077
BseXI GCAGC 2 cut(s) 465, 1046
BsgI GTGCAG 2 cut(s) 504, 759
Bsh1236I CGCG 1 cut(s) 166
BshFI GGCC 5 cut(s) 5, 170, 743, 1097, 1105
BshNI GGYRCC 2 cut(s) 1002, 1072
BshTI ACCGGT 1 cut(s) 999
BsiSI CCGG 1 cut(s) 1000
BslFI GGGAC 1 cut(s) 1010
BslI CCNNNNNNNGG 3 cut(s) 159, 160, 185
BsmAI GTCTC 3 cut(s) 76, 510, 791
BsmBI CGTCTC 1 cut(s) 791
BsmFI GGGAC 1 cut(s) 1010
BsnI GGCC 5 cut(s) 5, 170, 743, 1097, 1105
Bsp119I TTCGAA 1 cut(s) 543
Bsp1286I GDGCHC 1 cut(s) 1077
Bsp143I GATC 3 cut(s) 722, 877, 961
BspACI CCGC 4 cut(s) 166, 284, 287, 1020
BspANI GGCC 5 cut(s) 5, 170, 743, 1097, 1105
BspCNI CTCAG 2 cut(s) 94, 772
BspFNI CGCG 1 cut(s) 166
BspLI GGNNCC 5 cut(s) 136, 655, 998, 1004, 1074
BspPI GGATC 1 cut(s) 969
BspT104I TTCGAA 1 cut(s) 543
BspT107I GGYRCC 2 cut(s) 1002, 1072
BsrDI GCAATG 2 cut(s) 21, 538
BsrFI RCCGGY 1 cut(s) 999
BsrI ACTGG 2 cut(s) 220, 259
BssAI RCCGGY 1 cut(s) 999
BssECI CCNNGG 1 cut(s) 1068
BssMI GATC 3 cut(s) 722, 877, 961
Bst4CI ACNGT 3 cut(s) 233, 448, 927
Bst6I CTCTTC 1 cut(s) 515
BstBI TTCGAA 1 cut(s) 543
BstC8I GCNNGC 5 cut(s) 96, 168, 284, 910, 942
BstDEI CTNAG 3 cut(s) 81, 632, 759
BstDSI CCRYGG 1 cut(s) 1068
BstF5I GGATG 3 cut(s) 760, 1006, 1064
BstFNI CGCG 1 cut(s) 166
BstHHI GCGC 1 cut(s) 166
BstKTI GATC 3 cut(s) 725, 880, 964
BstMAI GTCTC 3 cut(s) 76, 510, 791
BstMBI GATC 3 cut(s) 722, 877, 961
BstMWI GCNNNNNNNGC 5 cut(s) 11, 91, 528, 941, 1094
BstNSI RCATGY 1 cut(s) 948
BstSFI CTRYAG 1 cut(s) 384
BstSLI GKGCMC 1 cut(s) 1077
BstUI CGCG 1 cut(s) 166
BstV1I GCAGC 2 cut(s) 465, 1046
BstX2I RGATCY 2 cut(s) 722, 961
BstXI CCANNNNNNTGG 1 cut(s) 751
BstYI RGATCY 2 cut(s) 722, 961
BsuRI GGCC 5 cut(s) 5, 170, 743, 1097, 1105
BtgI CCRYGG 1 cut(s) 1068
BtgZI GCGATG 1 cut(s) 583
BtsCI GGATG 3 cut(s) 760, 1006, 1064
BtsI GCAGTG 1 cut(s) 771
BtsIMutI CAGTG 1 cut(s) 771
Cac8I GCNNGC 5 cut(s) 96, 168, 284, 910, 942
CaiI CAGNNNCTG 1 cut(s) 265
CfoI GCGC 1 cut(s) 166
Cfr10I RCCGGY 1 cut(s) 999
Cfr13I GGNCC 3 cut(s) 168, 997, 1096
CseI GACGC 1 cut(s) 511
Csp6I GTAC 1 cut(s) 1003
CspAI ACCGGT 1 cut(s) 999
CspCI CAANNNNNGTGG 2 cut(s) 974, 1009
CviAII CATG 4 cut(s) 745, 945, 1078, 1107
CviQI GTAC 1 cut(s) 1003
DdeI CTNAG 3 cut(s) 81, 632, 759
DpnI GATC 3 cut(s) 724, 879, 963
DpnII GATC 3 cut(s) 722, 877, 961
EaeI YGGCCR 2 cut(s) 3, 1103
Eam1104I CTCTTC 1 cut(s) 515
EarI CTCTTC 1 cut(s) 515
Eco47I GGWCC 1 cut(s) 997
Eco57I CTGAAG 1 cut(s) 342
Esp3I CGTCTC 1 cut(s) 791
FaeI CATG 4 cut(s) 748, 948, 1081, 1110
FalI AAGNNNNNCTT 2 cut(s) 494, 526
FaqI GGGAC 1 cut(s) 1010
FatI CATG 4 cut(s) 744, 944, 1077, 1106
FauI CCCGC 2 cut(s) 159, 291
Fnu4HI GCNGC 3 cut(s) 287, 454, 1035
FokI GGATG 3 cut(s) 767, 993, 1051
Fsp4HI GCNGC 3 cut(s) 287, 454, 1035
FspBI CTAG 5 cut(s) 159, 207, 254, 567, 650
GlaI GCGC 1 cut(s) 165
GluI GCNGC 3 cut(s) 287, 454, 1035
HaeIII GGCC 5 cut(s) 5, 170, 743, 1097, 1105
HapII CCGG 1 cut(s) 1000
HgaI GACGC 1 cut(s) 511
HhaI GCGC 1 cut(s) 166
Hin1II CATG 4 cut(s) 748, 948, 1081, 1110
Hin6I GCGC 1 cut(s) 164
HinP1I GCGC 1 cut(s) 164
HincII GTYRAC 1 cut(s) 707
HindII GTYRAC 1 cut(s) 707
HindIII AAGCTT 1 cut(s) 906
HinfI GANTC 4 cut(s) 116, 345, 839, 956
HpaII CCGG 1 cut(s) 1000
HphI GGTGA 4 cut(s) 125, 421, 740, 1067
Hpy166II GTNNAC 2 cut(s) 707, 950
Hpy188I TCNGA 6 cut(s) 267, 307, 466, 527, 808, 961
Hpy188III TCNNGA 4 cut(s) 207, 334, 360, 369
Hpy8I GTNNAC 2 cut(s) 707, 950
Hpy99I CGWCG 1 cut(s) 527
HpyAV CCTTC 3 cut(s) 334, 399, 1109
HpyCH4III ACNGT 3 cut(s) 233, 448, 927
HpyCH4IV ACGT 2 cut(s) 709, 784
HpyCH4V TGCA 7 cut(s) 14, 67, 443, 485, 776, 793, 830
HpyF10VI GCNNNNNNNGC 5 cut(s) 11, 91, 528, 941, 1094
HpyF3I CTNAG 3 cut(s) 81, 632, 759
HpySE526I ACGT 2 cut(s) 709, 784
Hsp92II CATG 4 cut(s) 748, 948, 1081, 1110
HspAI GCGC 1 cut(s) 164
KpnI GGTACC 1 cut(s) 1006
Kzo9I GATC 3 cut(s) 722, 877, 961
LmnI GCTCC 1 cut(s) 653
Lsp1109I GCAGC 2 cut(s) 465, 1046
LweI GCATC 1 cut(s) 745
MaeI CTAG 5 cut(s) 159, 207, 254, 567, 650
MaeII ACGT 2 cut(s) 709, 784
MaeIII GTNAC 3 cut(s) 113, 569, 1055
MalI GATC 3 cut(s) 724, 879, 963
MboI GATC 3 cut(s) 722, 877, 961
MboII GAAGA 2 cut(s) 502, 672
MfeI CAATTG 1 cut(s) 687
MflI RGATCY 2 cut(s) 722, 961
MhlI GDGCHC 1 cut(s) 1077
MlsI TGGCCA 1 cut(s) 5
MluNI TGGCCA 1 cut(s) 5
MlyI GAGTC 1 cut(s) 110
MmeI TCCRAC 4 cut(s) 112, 457, 505, 1034
MnlI CCTC 4 cut(s) 210, 356, 733, 808
Mox20I TGGCCA 1 cut(s) 5
MscI TGGCCA 1 cut(s) 5
MslI CAYNNNNRTG 1 cut(s) 749
Msp20I TGGCCA 1 cut(s) 5
MspA1I CMGCKG 1 cut(s) 125
MspI CCGG 1 cut(s) 1000
MunI CAATTG 1 cut(s) 687
MvnI CGCG 1 cut(s) 166
MwoI GCNNNNNNNGC 5 cut(s) 11, 91, 528, 941, 1094
NdeII GATC 3 cut(s) 722, 877, 961
NlaIII CATG 4 cut(s) 748, 948, 1081, 1110
NlaIV GGNNCC 5 cut(s) 136, 655, 998, 1004, 1074
NmeAIII GCCGAG 1 cut(s) 30
NmuCI GTSAC 2 cut(s) 113, 1055
NspI RCATGY 1 cut(s) 948
NspV TTCGAA 1 cut(s) 543
PcsI WCGNNNNNNNCGW 1 cut(s) 873
PfeI GAWTC 3 cut(s) 345, 839, 956
PinAI ACCGGT 1 cut(s) 999
PkrI GCNGC 3 cut(s) 288, 455, 1036
PleI GAGTC 1 cut(s) 110
PpsI GAGTC 1 cut(s) 110
PsiI TTATAA 3 cut(s) 183, 558, 1050
PspN4I GGNNCC 5 cut(s) 136, 655, 998, 1004, 1074
PspPI GGNCC 3 cut(s) 168, 997, 1096
PstNI CAGNNNCTG 1 cut(s) 265
PsuI RGATCY 2 cut(s) 722, 961
PvuII CAGCTG 1 cut(s) 125
RsaI GTAC 1 cut(s) 1004
RsaNI GTAC 1 cut(s) 1003
RseI CAYNNNNRTG 1 cut(s) 749
SatI GCNGC 3 cut(s) 287, 454, 1035
Sau3AI GATC 3 cut(s) 722, 877, 961
Sau96I GGNCC 3 cut(s) 168, 997, 1096
SchI GAGTC 1 cut(s) 110
SduI GDGCHC 1 cut(s) 1077
SetI ASST 6 cut(s) 127, 620, 651, 712, 787, 910
SfaNI GCATC 1 cut(s) 745
SfcI CTRYAG 1 cut(s) 384
SfuI TTCGAA 1 cut(s) 543
SinI GGWCC 1 cut(s) 997
SmiMI CAYNNNNRTG 1 cut(s) 749
SpeI ACTAGT 1 cut(s) 566
SsiI CCGC 4 cut(s) 166, 284, 287, 1020
SspI AATATT 2 cut(s) 37, 316
SspMI CTAG 5 cut(s) 159, 207, 254, 567, 650
TaaI ACNGT 3 cut(s) 233, 448, 927
TaiI ACGT 2 cut(s) 712, 787
TaqI TCGA 4 cut(s) 543, 716, 738, 867
TauI GCSGC 1 cut(s) 289
TfiI GAWTC 3 cut(s) 345, 839, 956
TscAI CASTG 1 cut(s) 778
TseFI GTSAC 2 cut(s) 113, 1055
TseI GCWGC 2 cut(s) 453, 1034
Tsp45I GTSAC 2 cut(s) 113, 1055
TspDTI ATGAA 5 cut(s) 291, 502, 761, 831, 914
TspGWI ACGGA 2 cut(s) 690, 1057
TspRI CASTG 1 cut(s) 778
VpaK11BI GGWCC 1 cut(s) 997
XapI RAATTY 3 cut(s) 269, 323, 637
XbaI TCTAGA 1 cut(s) 206
XceI RCATGY 1 cut(s) 948
XspI CTAG 5 cut(s) 159, 207, 254, 567, 650
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.